CN111466324A - Method for cultivating seawater pearls by using pearl cell sap - Google Patents
Method for cultivating seawater pearls by using pearl cell sap Download PDFInfo
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- CN111466324A CN111466324A CN202010369448.6A CN202010369448A CN111466324A CN 111466324 A CN111466324 A CN 111466324A CN 202010369448 A CN202010369448 A CN 202010369448A CN 111466324 A CN111466324 A CN 111466324A
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- 239000011049 pearl Substances 0.000 title claims abstract description 182
- 239000013535 sea water Substances 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000490567 Pinctada Species 0.000 claims abstract description 72
- 235000013372 meat Nutrition 0.000 claims abstract description 35
- 210000001124 body fluid Anatomy 0.000 claims abstract description 21
- 239000010839 body fluid Substances 0.000 claims abstract description 21
- 235000015097 nutrients Nutrition 0.000 claims abstract description 18
- 229930003779 Vitamin B12 Natural products 0.000 claims abstract description 15
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 claims abstract description 15
- 239000011715 vitamin B12 Substances 0.000 claims abstract description 15
- 235000019163 vitamin B12 Nutrition 0.000 claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 13
- 235000015170 shellfish Nutrition 0.000 claims abstract description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 11
- 239000008103 glucose Substances 0.000 claims abstract description 11
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 230000001788 irregular Effects 0.000 claims abstract description 4
- 239000000843 powder Substances 0.000 claims description 27
- 238000003756 stirring Methods 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 238000002156 mixing Methods 0.000 claims description 12
- 239000002994 raw material Substances 0.000 claims description 11
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 claims description 8
- 239000008213 purified water Substances 0.000 claims description 8
- 210000004872 soft tissue Anatomy 0.000 claims description 7
- 238000005507 spraying Methods 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 241000237502 Ostreidae Species 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 6
- 235000020636 oyster Nutrition 0.000 claims description 6
- 238000012136 culture method Methods 0.000 claims description 5
- 235000005956 Cosmos caudatus Nutrition 0.000 claims description 4
- 244000293323 Cosmos caudatus Species 0.000 claims description 4
- 239000000706 filtrate Substances 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 239000011573 trace mineral Substances 0.000 claims description 4
- 235000013619 trace mineral Nutrition 0.000 claims description 4
- 229930003231 vitamin Natural products 0.000 claims description 4
- 235000013343 vitamin Nutrition 0.000 claims description 4
- 239000011782 vitamin Substances 0.000 claims description 4
- 229940088594 vitamin Drugs 0.000 claims description 4
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 4
- 210000002149 gonad Anatomy 0.000 claims description 3
- 238000000926 separation method Methods 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 2
- 239000013078 crystal Substances 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 4
- 102000011782 Keratins Human genes 0.000 abstract description 2
- 108010076876 Keratins Proteins 0.000 abstract description 2
- 230000028327 secretion Effects 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 4
- 239000010984 cultured pearl Substances 0.000 description 3
- 239000013505 freshwater Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000005498 polishing Methods 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000004537 pulping Methods 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000005034 decoration Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011050 natural pearl Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/54—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
- A01K61/56—Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
- A01K61/57—Pearl seeds
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for cultivating seawater pearls by using pearl cell sap, which comprises the following steps: preparing shellfish meat extract, preparing pearl oyster body fluid extract, preparing glucose solution, preparing vitamin B12 nutrient solution, preparing pearl cell fluid, inoculating pearl oyster and fusing for 7 technical steps. The pearl cell liquid of the invention is a pearl complete nutrient solution, after the nutrient substance which is inoculated and fused enters the body of the pearl oyster, the nutrient solution can not generate rejection phenomenon, can be well fused and absorbed with the pearl oyster, stimulates the pearl oyster to secrete nacre secretion in the growth process, forms shell keratin crystal, and conglomerates to form pearl crystal. The method of the invention is used for cultivating and culturing pearl shells for 1 to 3 years to form irregular pearls without pearl nuclei with the grain diameter of 0.1 to 0.4 cm.
Description
Technical Field
The invention relates to a method for cultivating seawater pearls by using pearl cell sap, belonging to the technical field of pearl cultivation.
Background
The pearl of the invention refers to seawater pearl. Pearls have two functions since ancient times, namely the decoration function of jewelry products and the treatment function of medicaments loaded in Chinese pharmacopoeia. As jewelry products, pearls are required to be mellow and gorgeous and have large particle size and thick layer, and the naturally grown pearls are difficult to meet the requirements because the pearls need to be mellow in appearance, namely, the pearls are required to be mellow in nucleus, namely, the pearls are inserted into pearl shells to grow the mellow pearls. Therefore, at present, the pearl nucleus is inserted into the pearl cultured artificially and then the pearl grows under the natural growth condition, so that the pearl shell secretes a pearl layer to wrap the pearl nucleus to form the pearl, and the longer the growth time is, the thicker the pearl layer is. However, the pearl cultured by inserting the nucleus can not meet the requirements of the pearl item in the Chinese pharmacopoeia, because in the artificially cultured pearl, the pearl nucleus occupies most of the volume of the whole pearl, the pearl layer only occupies a small part of the volume of the whole pearl, in the pearl powder prepared by the artificially cultured pearl, the impurity content is too large, the impurity comes from the pearl nucleus, the pearl nucleus is processed by the shell of the fresh water shellfish, the main component is calcium carbonate, the nacre is crystallized by the component secreted by the pearl shellfish, the processing of the pearl powder at present can not reach the industrial level of separating the pearl nucleus from the pearl nucleus, and the cost is huge if the pearl nucleus is separated actually. Therefore, at present, the commercially available pearl powder is not actually pearl powder prepared from real pearls, most of the pearl powder is processed by using a part of the innermost layer of pearl shells, namely pearl layer powder, which is actually pearl shell powder, but is only the innermost layer of pearl shells. The pearl powder and the pearl powder with the real meaning have different effects, because the content difference of the two components is huge, most of the pearl powder sold in the market is fresh water pearl powder, and the fresh water pearl and the seawater pearl are from different shellfish because of different production environments, so the cultured pearls have different components and different effects. How to cultivate pearls suitable for medicinal use is an important subject in the pearl cultivation industry, and the invention is provided for solving the technical problem.
Disclosure of Invention
The invention aims to provide a method for cultivating seawater pearls by using pearl cell sap.
The purpose of the invention is realized by the following technical measures: a method for cultivating seawater pearls by using pearl cell sap comprises the following specific technical steps:
(1) Preparing a pearl shell extracting solution:
Taking pearl shell, removing rough shell of pearl shell, cleaning, crushing into fine powder of 200-300 meshes, mixing pearl shell fine powder and clean seawater in a proportion of 1: 4-6, adding clean seawater, soaking for more than 10 hours while stirring at irregular time to dissolve and release trace element components which can be dissolved out from the pearl shell powder, filtering to obtain filtrate, heating and concentrating to 2/5 of the original liquid amount to obtain pearl shell extract for later use;
(2) Preparing a shellfish meat extracting solution:
Washing pearl oyster meat, wherein the weight ratio of the pearl oyster meat to clean seawater is 1: 4-6, adding clean seawater into the pearl oyster meat, grinding the pearl oyster meat into oyster meat pulp in a pulp grinder, and centrifuging the oyster meat pulp in a centrifuge to obtain a centrifugate, namely oyster meat extract for later use;
(3) Preparing a pearl shell body fluid extracting solution:
Taking body fluid of pearl oyster, and mixing the body fluid with the following components in a ratio of 1: 5-7, adding clean seawater, and uniformly stirring in a stirrer to obtain a pearl shell body fluid extract for later use;
(4) Preparing glucose liquid:
Mixing glucose powder and purified water in a proportion of 1: 3-4.5, adding water into glucose, and uniformly stirring to obtain glucose liquid for later use;
(5) Preparing a vitamin B12 nutrient solution:
Mixing a vitamin B12 raw material and purified water in a proportion of 1: 3-4.5, adding water into the vitamin B12 raw material, and uniformly stirring to obtain a vitamin 12 nutrient solution for later use;
(6) Preparing pearl cell liquid:
The raw material formula is as follows:
1-3 parts of pearl shell extract and 2-5 parts of shellfish meat extract
1-3 parts of pearl shell body fluid extract and 2-6 parts of glucose solution
1-3 parts of vitamin B12 nutrient solution,
Mixing the above materials, and stirring to obtain Margarita cell sap;
(7) Inoculating and fusing pearl oyster: separating the upper shell and the lower shell of the pearl oyster by 1-5 mm by using a conventional nucleus inserting step, sucking the pearl cell sap in a separated conchiole slit by using a syringe, dripping the pearl cell sap into the outer permeable membrane or the soft tissue of the conchiole or the soft tissue of the gonad of the pearl oyster or spraying the pearl cell sap into a trace sprayer, wherein the amount of the pearl cell sap is 0.1-0.3 ml, completing all operations within 3 minutes from the separation of the pearl oyster shell to the dripping or spraying of the pearl cell sap, quickly placing the pearl oyster inoculated with the pearl cell sap into a container of seawater with the same salinity and the same temperature as the seawater of a culture field for pre-culturing for 5-10 days, and transferring the pearl oyster inoculated with the pearl cell sap into the culture field for culturing for 1-3 years by using a conventional culture method after the pre-culturing.
The pearl cell liquid is a pearl full-ingredient nutrient liquid which is taken from pearl shell, therefore, after the pearl cell liquid as an inoculated and fused nutrient substance enters the body of the pearl shell, the pearl cell liquid can not generate rejection phenomenon, after the pearl cell liquid is inoculated on the outer permeable membrane of the pearl shell or the soft tissue of the horn of the shell or the soft tissue of the gonad, the pearl cell liquid can be well fused and absorbed with the pearl shell, the pearl shell is stimulated to secrete nacre secretion in the growth process, the keratin crystal is formed, the pearl crystal is formed by coalescence, and the obtained pearl crystal is similar to natural pearl because of no nucleus. The pearl without pearl nucleus in irregular shape with the grain diameter of 0.1-0.4 cm is formed in the pearl shells cultivated for 1-3 years by the method, and the pearl without pearl nucleus can not be completely round, can be used as jewelry and most of the pearl is used as medicinal or Chinese medicinal raw materials. The method for cultivating the seedless pearls is simple and convenient, easy to implement, obvious in effect, low in cultivation cost and obvious in economic effect. In order to verify the technical effect of the invention, a comparison test is carried out in 2016, the cultivation and culture test of the invention is carried out in a cultivation area of one mu of water surface, a comparison cultivation and culture test of a traditional cultivation mode is carried out in the same cultivation area of the other mu of water surface, the cultivation and culture test of the invention is carried out according to the method, the comparison cultivation and culture test is carried out according to the traditional nucleus insertion cultivation and culture method, the cultivation process is the same, the cultivation areas are the same, the cultivation time is 1-3 years, the sampling comparison is carried out in the same time periods of one year, two years and three years respectively, the comparison indexes are the inoculation pearl rate, the pearl forming rate and the survival rate, 200 pearl oysters are respectively sampled randomly, in the first year, among 200 pearl oysters in the test group of the invention, 173 years are live pearl oysters, 27 dead pearl oysters, 171 pearl oysters among the 173 live pearl oysters contain pearls, the average value of the pearl particle size is 1 mm, the survival rate is 86.5%, the pearl forming rate is 98.8%, the pearl inoculation rate is 98.8%, the second year test and the third year test are carried out according to the test method, the differences of the survival rate, the pearl forming rate and the pearl inoculation rate are not large, and the average value of the pearl particle size is increased to 2 mm and 4 mm. 139 of 200 pearl shells in the first year control group are live pearl shells and 61 dead pearl shells, and 112 of the 139 live pearl shells contain pearls, because the pearl grain diameters have no contrast meaning, the pearl grain diameter is not measured, the survival rate is 69.5%, the pearl forming rate is 80.6%, and the pearl inoculating rate is 80.6%. The second year test and the third year test are carried out according to the test method, the survival rate, the pearl forming rate and the pearl inoculation rate are not greatly different, and the changed index is that the average value of the pearl particle size is increased. The comparison result shows that the pearl cell sap used by the method of the invention is the same-polarity substance as the pearl oyster, although the pearl cell sap is the same-polarity foreign substance, the pearl oyster has no rejection phenomenon, and the pearl oyster can have higher pearl forming rate, because the pearl oyster is not seriously injured in the process of dropping or spraying the pearl cell sap into or into the pearl cell, and meanwhile, the pearl cell sap is beneficial to the recovery of the injured pearl, and has high recovery coefficient, so that the pearl cell sap has higher survival rate, pearl forming rate and pearl inoculation rate.
Detailed Description
Example 1
(1) Preparing a pearl shell extracting solution:
Taking 1 kg of pearl oyster, mechanically polishing the rough shell of the pearl oyster to remove, cleaning and drying the rough shell, crushing the pearl oyster into fine powder of 200 meshes, adding 4 kg of clean seawater into the fine powder of the pearl oyster to soak the pearl oyster for 10 hours, wherein the seawater is sandy filtered seawater, stirring for 2-3 times every hour in the soaking process to dissolve and release trace element components which can be dissolved out in the pearl oyster shell powder, filtering to obtain filtrate, and heating and concentrating to obtain 0.8 kg of pearl oyster extracting solution for later use;
(2) Preparing a shellfish meat extracting solution:
Cleaning 1 kg of pearl shell meat, adding 4 kg of clean seawater into the pearl shell meat, grinding the pearl shell meat into shell meat pulp in a pulping machine, and centrifuging the shell meat pulp in a centrifugal machine to obtain 4 kg of centrifugate, namely shell meat extracting solution for later use;
(3) Preparing a pearl shell body fluid extracting solution:
Taking 1 kg of body fluid of the pearl oyster, adding 5 kg of clean seawater into the body fluid of the pearl oyster, and uniformly stirring in a stirrer to obtain a body fluid extract of the pearl oyster for later use;
(4) Preparing glucose liquid:
Taking 1 kg of glucose powder, adding 3 kg of purified water, and uniformly stirring to obtain glucose liquid for later use;
(5) Preparing a vitamin B12 nutrient solution:
Taking 1 kg of vitamin B12 raw material, adding 3 kg of purified water, and uniformly stirring to obtain vitamin 12 nutrient solution for later use;
(6) Preparing pearl cell liquid:
The raw material formula is as follows:
1 kilogram of pearl shell extracting solution and 2 kilograms of shellfish meat extracting solution
Pearl oyster body fluid extract 1 kg and glucose solution 2 kg
1 kilogram of vitamin B12 nutrient solution,
Mixing the above materials, and stirring to obtain Margarita cell sap;
(7) Inoculating and fusing pearl oyster: taking pearl oyster and operating by the conventional nucleus inserting step, separating the upper shell and the lower shell of the pearl oyster by 1-5 mm, sucking the pearl cell sap by a syringe in a separated shell slit, dripping 0.1 ml of pearl cell sap into an outer permeable membrane of the pearl oyster, finishing all operations within 3 minutes from the separation of the pearl oyster to the dripping or spraying of the pearl cell sap, quickly placing the pearl oyster inoculated with the pearl cell sap into a container of seawater with the same salinity and the same temperature as the seawater of a culture field for pre-culture for 5 days, and transferring the pearl oyster inoculated with the pearl cell sap into the culture field for culture for 1-3 years by the conventional culture method after the pre-culture.
Example 2
(1) Preparing a pearl shell extracting solution:
Taking 1 kg of pearl oyster, mechanically polishing the rough shell of the pearl oyster to remove, cleaning and drying the rough shell, crushing the pearl oyster into fine powder of 300 meshes, adding 6 kg of clean seawater into the fine powder of the pearl oyster to soak the pearl oyster for 15 hours, wherein the seawater is sandy filtered seawater, stirring for 4-5 times every hour in the soaking process to dissolve and release trace element components which can be dissolved out in the pearl oyster shell powder, filtering to obtain filtrate, and heating and concentrating to obtain 1.8 kg of pearl oyster extracting solution for later use;
(2) Preparing a shellfish meat extracting solution:
Cleaning 1 kg of pearl shell meat, adding 6 kg of clean seawater into the pearl shell meat, grinding the pearl shell meat into shell meat pulp in a pulping machine, and centrifuging the shell meat pulp in a centrifuge to obtain 6 kg of centrifugate, namely shell meat extracting solution for later use;
(3) Preparing a pearl shell body fluid extracting solution:
Taking 1 kg of body fluid of the pearl oyster, adding 7 kg of clean seawater into the body fluid of the pearl oyster, and uniformly stirring in a stirrer to obtain a body fluid extract of the pearl oyster for later use;
(4) Preparing glucose liquid:
Taking 1 kg of glucose powder, adding 4.5 kg of purified water, and uniformly stirring to obtain glucose liquid for later use;
(5) Preparing a vitamin B12 nutrient solution:
1 kg of vitamin B12 raw material is taken, 4.5 kg of purified water is added, and the mixture is stirred uniformly to obtain vitamin 12 nutrient solution for later use;
(6) Preparing pearl cell liquid:
The raw material formula is as follows:
1 kilogram of pearl shell extracting solution and 2 kilograms of shellfish meat extracting solution
Pearl oyster body fluid extract 1 kg and glucose solution 2 kg
1 kilogram of vitamin B12 nutrient solution,
Mixing the above materials, and stirring to obtain Margarita cell sap;
(7) Inoculating and fusing pearl oyster: taking pearl oyster and operating by a conventional nucleus inserting step, separating the upper shell and the lower shell of the pearl oyster by 1-5 mm, sucking the pearl cell sap by a trace sprayer in a slit of the separated shells, spraying 0.3 ml of pearl cell sap into genital gland soft tissues of the pearl oyster, beginning from the division of the pearl shells until the pearl cell sap is dripped or sprayed, finishing all operations within 3 minutes, quickly placing the pearl oyster inoculated with the pearl cell sap into a container of seawater with the same salinity and the same temperature as the seawater of a culture field for pre-culturing for 10 days, and transferring the pearl oyster inoculated with the pearl cell sap into the culture field for culturing for 1-3 years by a conventional culture method after the pre-culturing.
Claims (1)
1. A method for cultivating seawater pearls by using pearl cell sap is characterized by comprising the following specific technical steps:
(1) Preparing a pearl shell extracting solution:
Taking pearl shell, removing rough shell of pearl shell, cleaning, crushing into fine powder of 200-300 meshes, mixing pearl shell fine powder and clean seawater in a proportion of 1: 4-6, adding clean seawater, soaking for more than 10 hours while stirring at irregular time to dissolve and release trace element components which can be dissolved out from the pearl shell powder, filtering to obtain filtrate, heating and concentrating to 2/5 of the original liquid amount to obtain pearl shell extract for later use;
(2) Preparing a shellfish meat extracting solution:
Washing pearl oyster meat, wherein the weight ratio of the pearl oyster meat to clean seawater is 1: 4-6, adding clean seawater into the pearl oyster meat, grinding the pearl oyster meat into oyster meat pulp in a pulp grinder, and centrifuging the oyster meat pulp in a centrifuge to obtain a centrifugate, namely oyster meat extract for later use;
(3) Preparing a pearl shell body fluid extracting solution:
Taking body fluid of pearl oyster, and mixing the body fluid with the following components in a ratio of 1: 5-7, adding clean seawater, and uniformly stirring in a stirrer to obtain a pearl shell body fluid extract for later use;
(4) Preparing glucose liquid:
Mixing glucose powder and purified water in a proportion of 1: 3-4.5, adding water into glucose, and uniformly stirring to obtain glucose liquid for later use;
(5) Preparing a vitamin B12 nutrient solution:
Mixing a vitamin B12 raw material and purified water in a proportion of 1: 3-4.5, adding water into the vitamin B12 raw material, and uniformly stirring to obtain a vitamin 12 nutrient solution for later use;
(6) Preparing pearl cell liquid:
The raw material formula is as follows:
1-3 parts of pearl shell extract and 2-5 parts of shellfish meat extract
1-3 parts of pearl shell body fluid extract and 2-6 parts of glucose solution
1-3 parts of vitamin B12 nutrient solution,
Mixing the above materials, and stirring to obtain Margarita cell sap;
(7) Inoculating and fusing pearl oyster: separating the upper shell and the lower shell of the pearl oyster by 1-5 mm by using a conventional nucleus inserting step, sucking the pearl cell sap in a separated conchiole slit by using a syringe, dripping the pearl cell sap into the outer permeable membrane or the soft tissue of the conchiole or the soft tissue of the gonad of the pearl oyster or spraying the pearl cell sap into a trace sprayer, wherein the amount of the pearl cell sap is 0.1-0.3 ml, completing all operations within 3 minutes from the separation of the pearl oyster shell to the dripping or spraying of the pearl cell sap, quickly placing the pearl oyster inoculated with the pearl cell sap into a container of seawater with the same salinity and the same temperature as the seawater of a culture field for pre-culturing for 5-10 days, and transferring the pearl oyster inoculated with the pearl cell sap into the culture field for culturing for 1-3 years by using a conventional culture method after the pre-culturing.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207770A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Method for cultivating pearls by using various calcium amino acids |
| CN113207771A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Multiple amino acid calcium culture solution for culturing pearls |
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| CN101491228A (en) * | 2009-03-02 | 2009-07-29 | 广西中医学院 | Sea no-nucleus pearl incubation method |
| CN102144586A (en) * | 2011-03-26 | 2011-08-10 | 北海市源龙珍珠有限公司 | Method for cultivating emerald pearl |
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| CN113207771A (en) * | 2021-05-08 | 2021-08-06 | 北海市源龙珍珠有限公司 | Multiple amino acid calcium culture solution for culturing pearls |
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