CN107517899A - The sea water pearls breeding method of seedless pattra pearl - Google Patents

The sea water pearls breeding method of seedless pattra pearl Download PDF

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Publication number
CN107517899A
CN107517899A CN201710912022.9A CN201710912022A CN107517899A CN 107517899 A CN107517899 A CN 107517899A CN 201710912022 A CN201710912022 A CN 201710912022A CN 107517899 A CN107517899 A CN 107517899A
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China
Prior art keywords
pearl
acceptor
membrane tissue
shellfish
core position
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CN201710912022.9A
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Chinese (zh)
Inventor
宁定凤
梁秋萍
梁萍萍
俞艳阳
陈鸿霆
梁旺海
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Beihai Wang Hai Jewelry Co Ltd
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Beihai Wang Hai Jewelry Co Ltd
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Priority to CN201710912022.9A priority Critical patent/CN107517899A/en
Publication of CN107517899A publication Critical patent/CN107517899A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • A01K61/56Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels for pearl production
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

Present invention mainly discloses the sea water pearls breeding method of seedless pattra pearl.Key step:Take adult pearl shell to be cleaned for donor, pearl oyster meat China and foreign countries permeable membrane tissue is taken under clean environment, is cleaned with trinosin dilution;The membrane tissue that will pass through inside is cut into particulate and it is formed membrane tissue liquid of passing through inside with outer permeable membrane mucus;It is acceptor to take adult healthy seawater pearl shell, and lower valve on acceptor shellfish is separated into a gap under clean environment, and the membrane tissue liquid that will pass through inside is implanted into acceptor shellfish by permeable membrane outside acceptor shellfish;The time control of above-mentioned steps is in three minutes.The acceptor shellfish for implanting membrane tissue particulate of passing through inside is put into rapidly in clean seawater and temporarily supported, and was put into three hours in sea water culture environment and cultivates naturally.The trinosin dilution is to use 10 milliliters of commodity tribiofosfor injection, 10 milligrams of content, is formulated with 50 milliliters of sterilized waters.The pearl shell cultivated with the inventive method, no operation wound, survival rate is high, and yield is high, best in quality.

Description

The sea water pearls breeding method of seedless pattra pearl
Technical field
The present invention relates to the sea water pearls breeding method of seedless pattra pearl, belongs to sea water pearls Cultivating techniques field.
Background technology
At present, sea water pearls are cultivated based on slotting core cultivation, are placed into by the implantation pearl core in pearl shell of performing the operation suitable Breeding environment in, naturally cultivation allow pearl shell secrete nacre parcel pearl core and form pearl, this method passes through implantation The pearl that pearl core forms, pearl shell injury being easily caused, wound is excessive, influences survival rate, and for pearl layer compared with book, market value is relatively low, Yield is also low.And the pearl formed by being implanted into mantle piece cultivation, cultivation successfully report is yet there are no in marine pearl, Therefore, seek a kind of sea water pearls breeding method of seedless pattra pearl, for cultivating seedless sea water pearls, reduce cultivation into This, it is significant to improve the market competitiveness.
The content of the invention
The purpose of the present invention is the deficiency in the presence of being cultivated for current sea water pearls, there is provided a kind of seedless pattra pearl Sea water pearls breeding method so that the sea water pearls cultivated form more without nucleus pearl in a shellfish, so as to reduce cultivation Cost, improve the yield and quality of no nucleus pearl.
Foregoing invention purpose is achieved by the following technical solution, the sea water pearls breeding method bag of seedless pattra pearl Include following particular technique steps:
(1)It is donor to take an adult pearl shell, is cleaned, by operation pearl shell donor upper and lower shell point under clean environment Open, take the outer permeable membrane position in pearl oyster meat, outer permeable membrane epidermis is removed, membrane tissue of passing through inside is obtained, uses trinosin Dilution is cleaned;
(2)Donor membrane tissue of passing through inside is cut to the membrane tissue particulate of passing through inside that particle diameter is 0.1-0.6 millimeters with tissue granulate equipment, outside Permeable membrane tissue particles form membrane tissue liquid of passing through inside with outer permeable membrane mucus;
(3)It is acceptor to take adult healthy seawater pearl shell, and the upper lower valve of acceptor shellfish is separated into a gap under clean environment, will Membrane tissue liquid pass through inside by permeable membrane outside acceptor shellfish, is implanted into the first core position, the second core position and the 3rd core position of acceptor shellfish, implantation it is suitable Sequence is the second core position, the 3rd core position, the first core position, is only implanted into the second core position in order to improve survival rate, during implantation or is only implanted into Three core positions are only implanted into the second core position and the 3rd core position, and the membrane tissue particulate loading of passing through inside of implantation is outside each core position implantation 1-5 grains Permeable membrane tissue particles;
(4)The time control of above three step is completed in three minutes;
(5)The acceptor shellfish for implanting membrane tissue particulate of passing through inside is put into rapidly in clean seawater and temporarily supported, and is put into sea in three hours Cultivated naturally in water breeding environment;
The trinosin dilution is to use 10 milliliters of commodity tribiofosfor injection, 10 milligrams of content, with 50 Milliliter sterilized water is formulated.
The present invention of above-mentioned measure is taken, extracts the biological tissue in donor pearl shell, is prepared into tissue particles liquid, then will In the related core position of biological tissue particulate implantation acceptor pearl shell, then cultivated under natural environment, using breeding method of the present invention Pearl shell, no operation wound, survival rate is high, survival rate to more than 60%, with multiple seedless treasure in each pearl shell Pearl, the quantity without nucleus pearl is between 2-8 in each pearl shell, and pearl thickness is real, and surface is glittering and translucent, light contemporary literature history.
Embodiment
Embodiment 1
(1)Trinosin dilution is first prepared, takes the trinosin note that 10 milliliters of contents are 10 milligrams Liquid is penetrated, adds in 50 milliliters of sterilized waters and stirs, obtain trinosin dilution;An adult pearl shell is taken again to supply Body, cleaned with physiological saline, the upper and lower shell of pearl shell donor is separated by performing the operation under clean environment, taken in pearl oyster meat Outer permeable membrane position, outer permeable membrane epidermis is removed, obtains outer permeable membrane biological tissue, is rinsed with trinosin dilution dry Only;
(2)Donor membrane tissue of passing through inside is cut to the membrane tissue particulate of passing through inside that particle diameter is 0.1-0.6 millimeters with tissue granulate equipment, outside Permeable membrane tissue particles form membrane tissue liquid of passing through inside with outer permeable membrane mucus;
(3)It is acceptor to take adult healthy seawater pearl shell, and the upper lower valve of acceptor shellfish is separated into a gap under clean environment, is used Suction pipe suction is passed through inside membrane tissue liquid, and the first core of acceptor shellfish is implanted into from the outer permeable membrane of acceptor shellfish by the suction pipe membrane tissue liquid that will pass through inside Position, the second core position and the 3rd core position, the order of implantation are first to be implanted into the second core position to be secondly implanted into first afterwards again in the 3rd core position of implantation Core position, the membrane tissue particulate loading of passing through inside of each core position implantation is 1-6 grains;
(4)The time control of above three step is completed in three minutes;
(5)The acceptor shellfish for implanting membrane tissue particulate of passing through inside is put into rapidly in clean seawater and temporarily supported, and is put into sea in three hours Cultivated naturally in water breeding environment.
Embodiment 2
Except step(3)Outside, remaining step is same as Example 1;
(3)It is acceptor to take adult healthy seawater pearl shell, and the upper lower valve of acceptor shellfish is separated into a gap under clean environment, is used Suction pipe suction is passed through inside membrane tissue liquid, and the second core of acceptor shellfish is implanted into from the outer permeable membrane of acceptor shellfish by the suction pipe membrane tissue liquid that will pass through inside The membrane tissue particulate loading of passing through inside of position implantation is 1-6 grains.
Embodiment 3
Except step(3)Outside, remaining step is same as Example 1;
(3)It is acceptor to take adult healthy seawater pearl shell, and the upper lower valve of acceptor shellfish is separated into a gap under clean environment, is used Suction pipe suction is passed through inside membrane tissue liquid, and the 3rd core of acceptor shellfish is implanted into from the outer permeable membrane of acceptor shellfish by the suction pipe membrane tissue liquid that will pass through inside Position, the membrane tissue particulate loading of passing through inside of implantation is 1-6 grains.
Embodiment 4
Except step(3)Outside, remaining step is same as Example 1;
(3)It is acceptor to take adult healthy seawater pearl shell, and the upper lower valve of acceptor shellfish is separated into a gap under clean environment, is used Suction pipe suction is passed through inside membrane tissue liquid, and the second core of acceptor shellfish is implanted into from the outer permeable membrane of acceptor shellfish by the suction pipe membrane tissue liquid that will pass through inside Position and the 3rd core position, the order of implantation are first to be implanted into the second core position to be secondly implanted into the 3rd core position, the outer permeable membrane of each core position implantation Tissue particles amount is 1-6 grains.
Experimental example 1
Sea water pearls cultivation is carried out by above-described embodiment 1 and routinely cultural method cultivates 3000 Hepu pearl shells, by 12 Cultivate within individual month, detected after harvest, survival rate 60%, pearl minimum number 1 in pearl shell, most 10, pearl is mellow and full, precious Pearl diameter 2-8 millimeters.
Experimental example 2
Sea water pearls cultivation is carried out by above-described embodiment 2 and routinely cultural method cultivates 3000 Hepu pearl shells, by 8 Cultivate within individual month, detected after harvest, survival rate 60%, pearl minimum number 1 in pearl shell, most 6, pearl is mellow and full, pearl Diameter 2-8 millimeters.
Experimental example 3
Sea water pearls cultivation is carried out by above-described embodiment 2 and routinely cultural method cultivates 3000 Hepu pearl shells, by 10 Cultivate within individual month, detected after harvest, survival rate 55%, pearl minimum number 1 in pearl shell, most 6, pearl is mellow and full, pearl Diameter 2-8 millimeters.
Experimental example 4
Sea water pearls cultivation is carried out by above-described embodiment 2 and routinely cultural method cultivates 3000 Hepu pearl shells, by 12 Cultivate within individual month, detected after harvest, survival rate 58%, pearl minimum number 1 in pearl shell, most 8, pearl is mellow and full, pearl Diameter 2-8 millimeters.

Claims (1)

1. the sea water pearls breeding method of seedless pattra pearl, it is characterized in that methods described includes following particular technique steps:
(1)It is donor to take an adult pearl shell, is cleaned, by operation pearl shell donor upper and lower shell point under clean environment Open, take the outer permeable membrane position in pearl oyster meat, outer permeable membrane epidermis is removed, membrane tissue of passing through inside is obtained, uses trinosin Dilution is cleaned;
(2)Donor membrane tissue of passing through inside is cut to the membrane tissue particulate of passing through inside that particle diameter is 0.1-0.6 millimeters with tissue granulate equipment, outside Permeable membrane tissue particles form membrane tissue liquid of passing through inside with outer permeable membrane mucus;
(3)It is acceptor to take adult healthy seawater pearl shell, and the upper lower valve of acceptor shellfish is separated into a gap under clean environment, will Membrane tissue liquid pass through inside by permeable membrane outside acceptor shellfish, is implanted into the first core position, the second core position and the 3rd core position of acceptor shellfish, implantation it is suitable Sequence is the second core position, the 3rd core position, the first core position, is only implanted into the second core position in order to improve survival rate, during implantation or is only implanted into Three core positions are only implanted into the second core position and the 3rd core position, and the membrane tissue particulate loading of passing through inside of implantation is outside each core position implantation 1-5 grains Permeable membrane tissue particles;
(4)The time control of above three step is completed in three minutes;
(5)The acceptor shellfish for implanting membrane tissue particulate of passing through inside is put into rapidly in clean seawater and temporarily supported, and is put into sea in three hours Cultivated naturally in water breeding environment;
The trinosin dilution is to use 10 milliliters of commodity tribiofosfor injection, 10 milligrams of content, with 50 Milliliter sterilized water is formulated.
CN201710912022.9A 2017-09-29 2017-09-29 The sea water pearls breeding method of seedless pattra pearl Pending CN107517899A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108935249A (en) * 2018-08-03 2018-12-07 广东荣辉珍珠养殖有限公司 Pearl breedinmg natural peral method and gained pearl
CN109588349A (en) * 2019-01-15 2019-04-09 北海汇善珠宝有限公司 A kind of golden pearl cultivation method
CN111466324A (en) * 2020-05-05 2020-07-31 北海市源龙珍珠有限公司 Method for cultivating seawater pearls by using pearl cell sap
CN111466325A (en) * 2020-05-05 2020-07-31 北海市源龙珍珠有限公司 Pearl cell liquid for cultivating seawater pearls
CN112586414A (en) * 2020-12-31 2021-04-02 广东尊鼎珍珠有限公司 Cultivation method of seedless seawater pearls

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JPH0746949A (en) * 1992-12-24 1995-02-21 Toan Hai Cultivating method for pearl by implanting core in reproductive bladder of abalone
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CN101491229A (en) * 2009-03-02 2009-07-29 广西中医学院 Vitro culturing method of pearl shell mantle tissue and culture medium
CN101491228A (en) * 2009-03-02 2009-07-29 广西中医学院 Sea no-nucleus pearl incubation method
CN103314912A (en) * 2013-07-03 2013-09-25 浙江海洋学院 Method of producing pearls on the basis of pearl shell mantel cell culture
JP2016174591A (en) * 2015-03-20 2016-10-06 有限会社松本真珠 Novel cultured pearl

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JPH0746949A (en) * 1992-12-24 1995-02-21 Toan Hai Cultivating method for pearl by implanting core in reproductive bladder of abalone
CN101361463A (en) * 2007-08-09 2009-02-11 普利科技有限公司 Pearl culture method
CN101491229A (en) * 2009-03-02 2009-07-29 广西中医学院 Vitro culturing method of pearl shell mantle tissue and culture medium
CN101491228A (en) * 2009-03-02 2009-07-29 广西中医学院 Sea no-nucleus pearl incubation method
CN103314912A (en) * 2013-07-03 2013-09-25 浙江海洋学院 Method of producing pearls on the basis of pearl shell mantel cell culture
JP2016174591A (en) * 2015-03-20 2016-10-06 有限会社松本真珠 Novel cultured pearl

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108935249A (en) * 2018-08-03 2018-12-07 广东荣辉珍珠养殖有限公司 Pearl breedinmg natural peral method and gained pearl
CN108935249B (en) * 2018-08-03 2021-03-19 广东荣辉珍珠养殖有限公司 Method for cultivating natural pearl by pearl shell and pearl obtained thereby
CN109588349A (en) * 2019-01-15 2019-04-09 北海汇善珠宝有限公司 A kind of golden pearl cultivation method
CN111466324A (en) * 2020-05-05 2020-07-31 北海市源龙珍珠有限公司 Method for cultivating seawater pearls by using pearl cell sap
CN111466325A (en) * 2020-05-05 2020-07-31 北海市源龙珍珠有限公司 Pearl cell liquid for cultivating seawater pearls
CN112586414A (en) * 2020-12-31 2021-04-02 广东尊鼎珍珠有限公司 Cultivation method of seedless seawater pearls

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Application publication date: 20171229