CN111450006A - Composition with multi-target acne removing effect and preparation method and application thereof - Google Patents
Composition with multi-target acne removing effect and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a composition with multi-target acne removing efficacy, and a preparation method and application thereof, wherein the composition comprises a radix sophorae flavescentis extract, a folium artemisiae argyi extract and a honeysuckle extract, wherein the radix sophorae flavescentis extract accounts for 25% -50%, the folium artemisiae argyi extract accounts for 25% -50%, and the honeysuckle extract accounts for 10% -25% of the mass ratio of the raw materials. The inhibition of the formula on the propionibacterium acnes reproduction is embodied in a multi-target form, and the formula can ensure the efficacy of inhibiting acne inflammation and the safety. The sophora flavescens, the folium artemisiae argyi and the honeysuckle extracts in the formula can inhibit the reproduction of propionibacterium acnes and can also inhibit propionibacterium acnes from releasing outer membrane vesicles, and the effect of inhibiting acne inflammation is achieved.
Description
Technical Field
The invention belongs to the technical field of cosmetic preparation, and particularly relates to a composition with multi-target acne removing efficacy, and a preparation method and application thereof.
Background
Acne is a chronic inflammatory disease occurring in the sebaceous glands, the occurrence of acne being closely related to the activity of propionibacterium acnes. Recent studies have found that propionibacterium acnes not only causes skin inflammation, but that the outer membrane vesicles released by it are more likely to cause intense inflammation.
Thus, although antibacterial drugs inhibit bacterial growth, the presence of outer membrane vesicles renders inflammation undedicable. Therefore, reducing the release of outer membrane vesicles while effectively inhibiting bacteria, and inhibiting inflammatory response is the right way to alleviate or cure acne.
At present, no report of drugs for inhibiting the release of the outer membrane vesicles of propionibacterium acnes exists, and therefore, a composition with multi-target acne removing efficacy is urgently needed in the field to effectively inhibit the release of the outer membrane vesicles of propionibacterium acnes.
Disclosure of Invention
This section is for the purpose of summarizing some aspects of embodiments of the invention and to briefly introduce some preferred embodiments. In this section, as well as in the abstract and the title of the invention of this application, simplifications or omissions may be made to avoid obscuring the purpose of the section, the abstract and the title, and such simplifications or omissions are not intended to limit the scope of the invention.
The present invention has been made keeping in mind the above and/or other problems occurring in the prior art.
Therefore, the invention aims to overcome the defects in the prior art and provide a composition with multi-target acne removing efficacy.
In order to solve the technical problems, the invention provides the following technical scheme: a composition with multi-target acne removing effect comprises a radix sophorae flavescentis extract, a folium artemisiae argyi extract and a honeysuckle extract, wherein the radix sophorae flavescentis extract accounts for 25% -50%, the folium artemisiae argyi extract accounts for 25% -50%, and the honeysuckle extract accounts for 10% -25% in percentage by mass of the raw materials.
The preparation method of the sophora flavescens extract comprises the steps of drying sophora flavescens to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 1-2 hours by using an 80-100% ethanol solution according to a material-liquid ratio of 1: 8-10 in terms of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 80-100% ethanol, filtering, combining two filtrates, carrying out rotary evaporation and concentration on the filtrates to obtain an extract, and carrying out freeze drying to obtain the sophora flavescens extract.
The preparation method of the folium artemisiae argyi extract comprises the steps of drying folium artemisiae argyi to constant weight, crushing, sieving by a 60-mesh sieve, performing reflux extraction for 1-2 hours by using 60-80% ethanol solution according to the material-liquid ratio of 1: 6-8 in g: m L, stirring continuously, filtering after the first extraction is finished, extracting residues once by using 60-80% ethanol, filtering, combining the two filtrates, performing rotary evaporation and concentration on the filtrates to obtain an extract, and performing freeze drying to obtain the folium artemisiae argyi extract.
The honeysuckle extract is prepared by drying honeysuckle to constant weight, crushing, sieving by a 60-mesh sieve, performing reflux extraction for 2-3 hours by using a 60-80% ethanol solution according to a material-liquid ratio of 1: 5-8 in g: m L, stirring continuously, filtering after the first extraction is finished, extracting residues by using 60-80% ethanol for one time, filtering, combining two filtrates, performing rotary evaporation and concentration on the filtrate to obtain an extract, and performing freeze drying to obtain the honeysuckle extract.
As a preferred embodiment of the composition with multi-target acne removing efficacy of the present invention, wherein: the composition with the multi-target acne removing effect comprises 25% of the sophora flavescens extract, 50% of the folium artemisiae argyi extract and 25% of the honeysuckle extract in percentage by mass of the raw materials.
Still another object of the present invention is to overcome the disadvantages of the prior art and provide a method for preparing a composition with multiple-target acne-removing efficacy.
The preparation method comprises the following steps of preparing a sophora flavescens extract, drying sophora flavescens to constant weight, crushing, sieving by a 60-mesh sieve, performing reflux extraction for 1: 8-10 according to a material-liquid ratio g: m L by using an 80-100% ethanol solution for 1-2 hours, stirring continuously, filtering after the first extraction is finished, extracting residues once by using 80-100% ethanol, filtering, combining the two filtrates, performing rotary evaporation and concentration on the filtrate to obtain an extract, performing freeze drying to obtain a folium artemisiae argyi extract, drying folium artemisiae argyi extract, crushing, sieving by a 60-mesh sieve, performing reflux extraction for 1: 6-8 according to a material-liquid ratio g: m L, stirring for 1-2 hours by using a 60-80% ethanol solution, filtering after the first extraction is finished, extracting residues once by using 60-80% ethanol, filtering, combining the filtrates, performing rotary evaporation and concentration on the filtrate to obtain an extract, drying to obtain a honeysuckle extract, preparing a honeysuckle extract, performing reflux extraction for 60-80% extract, performing continuous evaporation and concentration on the filtrate to obtain a 60-80% extractive solution, drying, performing continuous evaporation and concentration on the folium artemisiae argyi extract, drying, preparing a 60-80% extractive solution, drying, performing reflux extraction for 60-60% extractive for twice, mixing, and concentrating for 60% extractive for 10% extractive for 60% filtrate, and concentrating for 10% extractive for 10, and concentrating for 10% of the extractive for 60-60% of the extractive for 10, and concentrating for 10, obtaining a 60-60% extractive for 10% extractive for twice, and concentrating for 10% of the extractive for.
As a preferred embodiment of the preparation method of the composition with multi-target acne removing efficacy, the invention comprises the following steps: the composition with the multi-target acne removing effect comprises 25% of radix sophorae flavescentis extract, 50% of folium artemisiae argyi extract and 25% of honeysuckle extract in percentage by mass.
The invention also aims to overcome the defects in the prior art and provide application of the composition with the multi-target acne removing effect in cosmetics, wherein the composition with the multi-target acne removing effect comprises 25-50% of radix sophorae flavescentis extract, 25-50% of folium artemisiae argyi extract and 10-25% of honeysuckle extract in percentage by mass, and the safe and effective concentration range in the formula is 20-50 mug/m L.
As a preferable scheme of the application of the composition with the multi-target acne removing effect in cosmetics, the composition with the multi-target acne removing effect comprises 25% of radix sophorae flavescentis extract, 50% of folium artemisiae argyi extract and 25% of honeysuckle extract in percentage by mass of the extracts, and the safe and effective concentration range in the formula is 50 mu g/m L.
The invention has the beneficial effects that:
(1) the invention discloses a composition with multi-target-point acne removing effect and a preparation method and application thereof. The inhibition of the formula on the propionibacterium acnes reproduction is embodied in a multi-target form, and the formula can ensure the efficacy of inhibiting acne inflammation and the safety. The sophora flavescens, the folium artemisiae argyi and the honeysuckle extracts in the formula can inhibit the reproduction of propionibacterium acnes and can also inhibit propionibacterium acnes from releasing outer membrane vesicles, and the effect of inhibiting acne inflammation is achieved.
(2) The extracts of the radix sophorae flavescentis, the folium artemisiae argyi and the honeysuckle in the formula are firstly considered to solve the safety problem of the traditional Chinese medicine extracts, compared with a single extract of the radix sophorae flavescentis, the folium artemisiae argyi and the honeysuckle, the traditional Chinese medicine formula consisting of the radix sophorae flavescentis, the folium artemisiae argyi and the honeysuckle has a better inhibition effect on acne inflammation, the highest inhibition rate of the I L-8 is 79 percent, the inhibition rate is superior to the effect achieved by each single traditional Chinese medicine extract, acne inflammation can be safely inhibited in a multi-x 1 target spot mode, and multiple target spots and synergistic effects are shown.
(3) The preparation process is simple and is suitable for popularization and application.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without inventive exercise. Wherein:
FIG. 1 is a graph comparing cytotoxicity of extracts and combinations thereof in the practice of the invention.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention more comprehensible, specific embodiments thereof are described in detail below with reference to examples of the specification.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, but the present invention may be practiced in other ways than those specifically described and will be readily apparent to those of ordinary skill in the art without departing from the spirit of the present invention, and therefore the present invention is not limited to the specific embodiments disclosed below.
Furthermore, reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
The test method takes the inhibition of propionibacterium acnes, the inhibition of the release of outer membrane vesicles of propionibacterium acnes and the anti-inflammatory effect as indexes, and comprises the following steps:
the invention uses CCK-8 method to determine the non-cytotoxic concentration of the extract:THP-1 cells were seeded in a 96-well plate, and the concentration of THP-1 cells was adjusted to 2.5 × 10 with RPMI1640 medium containing 10% fetal bovine serum6∙ m L pieces-110 μ g of ∙ m L was added-1Or 30 μ g of ∙ m L-1The formula solution is cultured in an incubator with 5 percent CO2 at 37 ℃ for 24 hours, and the cell activity is measured by a CCK-8 kit. The cell activity of the blank group without the traditional Chinese medicine extract is 100 percent. The cell activity is more than or equal to 80 percent, and the concentration is nontoxic.
The absorbance method for detecting the inhibition of Propionibacterium acnes by the extract or formulation comprises adjusting the concentration of the bacterial suspension to about 1 × 10 by using a liquid medium containing the extract7cFu∙ mL-1Culturing in constant temperature incubator at 37 deg.C for 48 hr under anaerobic condition, and using the blank containing no extract as control. And measuring the light absorption value at 600nm by using an ultraviolet spectrophotometer.
Culturing Propionibacterium acnes in liquid culture medium containing the extract at 37 deg.C under 5% CO2 to logarithmic growth phase, adding the extract as experimental group, and adding no extract as blank control. Centrifuging at 5000g and 4 deg.C for 15min to obtain supernatant, ultrafiltering with 100-KDa fiber membrane for 30 times, filtering with 0.22 μm filter, centrifuging the filtrate at 150000g and 4 deg.C for 3h, removing supernatant, washing with PBS for 3 times, suspending in PBS to obtain outer membrane vesicle solution, measuring outer membrane vesicle protein content with BCA kit, and calculating vesicle inhibition rate according to the following formula:
THP-1 cells were seeded in 6-well plates with cell concentration adjusted to 1 × 106∙ m L pieces-1Adding propionibacterium acnes, culturing for 24h, and detecting the content of I L-8 in the cell culture supernatant with E L ISA kit, wherein the extract of Chinese medicinal materials and propionibacterium acnes are added as experimental groups, and the extract of Chinese medicinal materials and acne are not addedThe propionibacterium is used as a blank control group, the propionibacterium acnes is used as a ContRO L group without adding the traditional Chinese medicine extract, and the I L-8 inhibition rate is calculated according to the following formula to serve as an anti-inflammatory index:
example 1
The embodiment provides a traditional Chinese medicine extract with multi-target acne removing efficacy and a preparation method thereof:
(1) the preparation method of the sophora flavescens extract comprises the steps of drying sophora flavescens to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 1h by using 80% ethanol solution according to the material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 80% ethanol, filtering, combining filtrates obtained in two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the sophora flavescens extract;
the preparation method of the folium artemisiae argyi extract comprises the steps of drying folium artemisiae argyi to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 1h by using a 60% ethanol solution according to the material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 60% ethanol, filtering, combining filtrates obtained in the two times, performing rotary evaporation and concentration on the filtrate to obtain an extract, and performing freeze drying to obtain the folium artemisiae argyi extract;
the preparation method of the honeysuckle extract comprises the steps of drying honeysuckle to constant weight, crushing, sieving by a 60-mesh sieve, carrying out reflux extraction for 2 hours by using 60% ethanol solution according to the material-liquid ratio of g: m L as 1: 5, continuously stirring, filtering after the first extraction is finished, extracting residues by using 60% ethanol once, filtering, combining filtrates obtained in the two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the honeysuckle extract;
(2) mixing the sophora flavescens extract, the artemisia leaf extract and the honeysuckle extract prepared in the step (1) according to the mass ratio of 9: 9: 2, adding the mixture into propylene glycol, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 20 mug/m L, which is marked as 1 a;
(3) preparing radix Sophorae Flavescentis Chinese medicinal extract by adding radix Sophorae Flavescentis extract in (1) into propylene glycol, and controlling the concentration of radix Sophorae Flavescentis extract to be 20 μ g/m L, as 1 b;
(4) preparing folium Artemisiae Argyi traditional Chinese medicine extract by adding folium Artemisiae Argyi extract in (1) into propylene glycol, controlling folium Artemisiae Argyi extract concentration to 20 μ g/m L, and recording as 1 c;
(5) preparing a honeysuckle traditional Chinese medicine extract, namely adding the honeysuckle extract in the step (1) into propylene glycol, and controlling the concentration of the honeysuckle extract to be 20 mug/m L, which is recorded as 1 d;
(6) preparing a radix sophorae flavescentis-honeysuckle flower traditional Chinese medicine extract, namely adding the radix sophorae flavescentis extract and the honeysuckle flower extract prepared in the step (1) into propylene glycol according to the mass ratio of 9: 2, controlling the total concentration of the traditional Chinese medicine extract in the formula to be 20 mug/m L, and recording as 1 e;
(7) preparing the folium artemisiae argyi-honeysuckle traditional Chinese medicine extract, namely adding the folium artemisiae argyi extract and the honeysuckle extract prepared in the step (1) into propylene glycol according to the mass ratio of 9: 2, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 20 mug/m L, which is recorded as 1 f.
The results of the measurements are shown in table 1, using the inhibition of Propionibacterium acnes, the inhibition of Propionibacterium acnes outer membrane vesicle release, and the anti-inflammatory effect as indicators.
TABLE 1
The table 1 shows that the sophora flavescens extract, the artemisia leaf extract and the honeysuckle extract have a good effect of inhibiting propionibacterium acnes, the artemisia leaf extract has an effect of inhibiting the release of outer membrane vesicles of propionibacterium acnes, and the honeysuckle extract has an effect of enhancing an anti-inflammatory effect and reducing cytotoxicity, each extract has no cytotoxicity when acting alone, but has a poor effect of reducing I L-8, namely, the final anti-inflammatory effect is poor.
Example 2
The embodiment provides a traditional Chinese medicine extract with multi-target acne removing efficacy and a preparation method thereof:
(1) the preparation method of the sophora flavescens extract comprises the steps of drying sophora flavescens to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 1: 8 by using 80% ethanol solution according to the material-liquid ratio of g: m L for 1 hour, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 80% ethanol, filtering, combining filtrates obtained in two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the sophora flavescens extract;
the preparation method of the folium artemisiae argyi extract comprises the steps of drying folium artemisiae argyi to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 1h by using a 60% ethanol solution according to a material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 60% ethanol, filtering, combining filtrates obtained in the two times, performing rotary evaporation and concentration on the filtrate to obtain an extract, and performing freeze drying to obtain the folium artemisiae argyi extract;
the preparation method of the honeysuckle extract comprises the steps of drying honeysuckle to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 2 hours by using a 60% ethanol solution according to a material-liquid ratio of g: m L as 1: 5, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 60% ethanol, filtering, combining filtrates obtained in the two times, carrying out rotary evaporation and concentration on the filtrate to obtain an extract, and carrying out freeze drying to obtain the honeysuckle extract;
(2) mixing the sophora flavescens extract, the artemisia leaf extract and the honeysuckle extract prepared in the step (1) according to the mass ratio of 9: 9: 2, adding the mixture into propylene glycol, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 40 mu g/m L, which is recorded as 2 a;
(3) preparing radix Sophorae Flavescentis Chinese medicinal extract by adding radix Sophorae Flavescentis extract in (1) into propylene glycol, and controlling concentration of radix Sophorae Flavescentis extract to be 40 μ g/m L, as 2 b;
(4) preparing folium Artemisiae Argyi traditional Chinese medicine extract by adding folium Artemisiae Argyi extract in (1) into propylene glycol, controlling folium Artemisiae Argyi extract concentration to 40 μ g/m L, and recording as 2 c;
(5) preparing a honeysuckle traditional Chinese medicine extract, namely adding the honeysuckle extract in the step (1) into propylene glycol, and controlling the concentration of the honeysuckle extract to be 40 mug/m L, which is recorded as 2 d;
(6) preparing a radix sophorae flavescentis-honeysuckle flower traditional Chinese medicine extract, namely adding the radix sophorae flavescentis extract and the honeysuckle flower extract prepared in the step (1) into propylene glycol according to the mass ratio of 9: 2, controlling the total concentration of the traditional Chinese medicine extract in the formula to be 40 mug/m L, and recording as 2 e;
(7) preparing the folium artemisiae argyi-honeysuckle traditional Chinese medicine extract, namely adding the folium artemisiae argyi extract and the honeysuckle extract prepared in the step (1) into propylene glycol according to the mass ratio of 9: 2, and controlling the total concentration of 40 mug/m L in the traditional Chinese medicine extract in the formula as 1 f.
The results of the measurements are shown in Table 2, using Propionibacterium acnes-inhibiting, Propionibacterium acnes-inhibiting outer membrane vesicle release-inhibiting, and anti-inflammatory effects as indices.
TABLE 2
As can be seen from Table 2, when radix Sophorae Flavescentis and folium Artemisiae Argyi were used alone, the cytotoxicity was too high to be practical. The cytotoxicity of the traditional Chinese medicine extract is reduced after combination, and example 2 has the effects of inhibiting the reproduction of propionibacterium acnes and inhibiting bacteria from releasing outer membrane vesicles, and can play a better role in inhibiting acne inflammation than a single extract under the condition of no cytotoxicity.
Example 3
The embodiment provides a traditional Chinese medicine extract with multi-target acne removing efficacy and a preparation method thereof:
(1) the preparation method of the sophora flavescens extract comprises the steps of drying sophora flavescens to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 1h by using 80% ethanol solution according to the material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 80% ethanol, filtering, combining filtrates obtained in two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the sophora flavescens extract;
the preparation method of the folium artemisiae argyi extract comprises the steps of drying folium artemisiae argyi to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 1h by using a 60% ethanol solution according to the material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 60% ethanol, filtering, combining filtrates obtained in the two times, performing rotary evaporation and concentration on the filtrate to obtain an extract, and performing freeze drying to obtain the folium artemisiae argyi extract;
the preparation method of the honeysuckle extract comprises the steps of drying honeysuckle to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 2 hours by using a 60% ethanol solution according to a material-liquid ratio of g: m L and keeping stirring, filtering after the first extraction is finished, extracting residues once by using 60% ethanol, filtering, combining filtrates obtained in two times, carrying out rotary evaporation and concentration on the filtrate to obtain an extract, and carrying out freeze drying to obtain the honeysuckle extract.
(2) Adding the sophora flavescens extract, the artemisia leaf extract and the honeysuckle extract prepared in the step (1) into propylene glycol according to the mass ratio of 7: 7: 6, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 40 mu g/m L, which is recorded as 3 a.
(3) Preparing a sophora flavescens traditional Chinese medicine extract, namely adding the sophora flavescens extract prepared in the step (1) into propylene glycol, and controlling the concentration of the sophora flavescens extract to be 40 mu g/m L, which is recorded as 3 b;
(4) preparing folium Artemisiae Argyi traditional Chinese medicine extract by adding folium Artemisiae Argyi extract in (1) into propylene glycol, controlling folium Artemisiae Argyi extract concentration to 40 μ g/m L, and recording as 3 c;
(5) preparing a honeysuckle traditional Chinese medicine extract, namely adding the honeysuckle extract in the step (1) into propylene glycol, and controlling the concentration of the honeysuckle extract to be 40 mug/m L, which is recorded as 4 d;
(6) preparing a radix sophorae flavescentis-honeysuckle flower traditional Chinese medicine extract, namely adding the radix sophorae flavescentis extract and the honeysuckle flower extract prepared in the step (1) into propylene glycol according to the mass ratio of 7: 6, controlling the total concentration of the traditional Chinese medicine extract in the formula to be 40 mug/m L, and recording as 4 e;
(7) preparing folium artemisiae argyi-honeysuckle traditional Chinese medicine extract, namely adding the folium artemisiae argyi extract and the honeysuckle extract prepared in the step (1) into propylene glycol according to the mass ratio of 7: 6, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 40 mug/m L, which is recorded as 4 f.
The results of the measurements are shown in Table 3, using Propionibacterium acnes-inhibiting, Propionibacterium acnes-inhibiting outer membrane vesicle release-inhibiting, and anti-inflammatory effects as indices.
TABLE 3
As can be seen from Table 3, when radix Sophorae Flavescentis and folium Artemisiae Argyi were used alone, the cytotoxicity was too high to be practical. The traditional Chinese medicine extract has reduced cytotoxicity after combination, has the effects of inhibiting the reproduction of propionibacterium acnes and inhibiting bacteria from releasing outer membrane vesicles, and can play a better role in inhibiting acne inflammation than a single extract under the condition of no cytotoxicity. Fig. 1 is a comparison graph of cytotoxicity of extracts and combinations thereof, and it can be seen from fig. 1 that cytotoxicity is generated at a lower concentration when a certain herb extract is used alone, and irritation may be generated to the skin, which makes practical application difficult. The extract combination can improve the application concentration of the extract, and can achieve better effect on relieving acne inflammation. The traditional Chinese medicine extract has no additive effect on cytotoxicity, and the formula not only reduces the cytotoxicity, but also has the effects of inhibiting the reproduction of propionibacterium acnes and inhibiting bacteria from releasing outer membrane vesicles, and can effectively inhibit acne inflammation.
Example 4
The embodiment provides a traditional Chinese medicine extract with multi-target acne removing efficacy and a preparation method thereof:
(1) the preparation method of the sophora flavescens extract comprises the steps of drying sophora flavescens to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 1h by using 80% ethanol solution according to the material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 80% ethanol, filtering, combining filtrates obtained in two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the sophora flavescens extract;
the preparation method of the folium artemisiae argyi extract comprises the steps of drying folium artemisiae argyi to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 1h by using a 60% ethanol solution according to the material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 60% ethanol, filtering, combining filtrates obtained in the two times, performing rotary evaporation and concentration on the filtrate to obtain an extract, and performing freeze drying to obtain the folium artemisiae argyi extract;
the preparation method of the honeysuckle extract comprises the steps of drying honeysuckle to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 2 hours by using a 60% ethanol solution according to a material-liquid ratio of g: m L and keeping stirring, filtering after the first extraction is finished, extracting residues once by using 60% ethanol, filtering, combining filtrates obtained in two times, carrying out rotary evaporation and concentration on the filtrate to obtain an extract, and carrying out freeze drying to obtain the honeysuckle extract.
(2) Adding the sophora flavescens extract, the artemisia leaf extract and the honeysuckle extract prepared in the step (1) into propylene glycol according to the mass ratio of 7: 7: 6, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 80 mug/m L, which is recorded as 4 a.
(3) Preparing radix Sophorae Flavescentis Chinese medicinal extract by adding radix Sophorae Flavescentis extract in (1) into propylene glycol, and controlling the concentration of radix Sophorae Flavescentis extract to 80 μ g/m L, as 4 b.
(4) Preparing folium Artemisiae Argyi traditional Chinese medicine extract by adding folium Artemisiae Argyi extract in (1) into propylene glycol, controlling folium Artemisiae Argyi extract concentration to 80 μ g/m L, and recording as 4 c.
(5) And (2) preparing a honeysuckle traditional Chinese medicine extract, namely adding the honeysuckle extract in the step (1) into propylene glycol, and controlling the concentration of the honeysuckle extract to be 80 mu g/m L, which is recorded as 4 d.
(6) And (2) preparing a radix sophorae flavescentis-honeysuckle flower traditional Chinese medicine extract, namely adding the radix sophorae flavescentis extract and the honeysuckle flower extract prepared in the step (1) into propylene glycol according to the mass ratio of 7: 6, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 80 mug/m L, which is recorded as 4 e.
(7) Preparing folium artemisiae argyi-honeysuckle traditional Chinese medicine extract, namely adding the folium artemisiae argyi extract and the honeysuckle extract prepared in the step (1) into propylene glycol according to the mass ratio of 7: 6, and controlling the total concentration of the traditional Chinese medicine extract in the formula to be 80 mug/m L, which is recorded as 4 f.
The results of the measurements are shown in Table 4, using Propionibacterium acnes-inhibiting, Propionibacterium acnes-inhibiting outer membrane vesicle release-inhibiting, and anti-inflammatory effects as indices.
TABLE 4
It can be seen that the traditional Chinese medicine extract formula has a good antibacterial effect and also plays a certain role in inhibiting the release of vesicles and I L-8, but cell death is caused by excessively high addition amount, and the addition amount may damage human skin, so that practical application is difficult.
Example 5
The embodiment provides a traditional Chinese medicine extract formula and a preparation method thereof:
(1) the preparation method of the angelica sinensis extract comprises the steps of drying angelica sinensis to constant weight, crushing, sieving by a 60-mesh sieve, carrying out reflux extraction for 1h by using 80% ethanol solution according to the material-liquid ratio of g: m L for 1: 6, stirring continuously, filtering after the first extraction is finished, extracting residues by using 80% ethanol for one time, filtering, combining filtrates obtained in the two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the angelica sinensis extract;
the preparation method of the lotus leaf extract comprises the steps of drying lotus leaves to constant weight, crushing, sieving by a 60-mesh sieve, carrying out reflux extraction for 1h by using 80% ethanol solution according to the material-liquid ratio of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues by using 80% ethanol once, filtering, combining filtrates obtained in the two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the lotus leaf extract;
the preparation method of the mulberry leaf extract comprises the steps of drying mulberry leaves to constant weight, crushing, sieving with a 60-mesh sieve, carrying out reflux extraction for 3 hours by using 80% ethanol solution according to the material-liquid ratio of 1: 8 in terms of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 80% ethanol, filtering, combining filtrates obtained in the two times, carrying out rotary evaporation and concentration on the filtrate to obtain extract, and carrying out freeze drying to obtain the mulberry leaf extract.
(2) Adding the angelica sinensis extract, the lotus leaf extract and the mulberry leaf extract prepared in the step (1) into propylene glycol according to the mass ratio of 4: 3: 3, and controlling the total concentration of the Chinese medicinal extract in the formula to be 20 mu g/m L, which is recorded as 5 a.
(2) Preparing Chinese angelica extract, namely adding the Chinese angelica extract prepared in the step (1) into propylene glycol, and controlling the concentration of the Chinese angelica extract to be 20 mu g/m L, which is recorded as 5 b.
(3) And (2) preparing a lotus leaf traditional Chinese medicine extract, namely adding the lotus leaf extract prepared in the step (1) into propylene glycol, and controlling the concentration of the lotus leaf extract to be 20 mu g/m L, which is recorded as 5 c.
(4) And (3) preparing a mulberry leaf traditional Chinese medicine extract, namely adding the mulberry leaf extract prepared in the step (1) into propylene glycol, and controlling the concentration of the mulberry leaf extract to be 20 mu g/m L, wherein the concentration is recorded as 5 d.
The results of the measurements are shown in Table 5, using Propionibacterium acnes-inhibiting, Propionibacterium acnes-inhibiting outer membrane vesicle release-inhibiting, and anti-inflammatory effects as indices.
TABLE 5
From table 5, it can be seen that the formula of the traditional Chinese medicine extract, angelica sinensis has a weak vesicle inhibition effect, lotus leaves have a weak bacteriostatic effect, and folium mori has two effects but has a weak effect. 5a extract has high cytotoxicity and weak anti-acne inflammation effect, and is difficult to have practical application.
Example 6
On the basis of example 3 (the total concentration of the traditional Chinese medicine extract in the formula is controlled to be 40 mu g/m L), the influence of the compounding ratio of the sophora flavescens extract, the artemisia leaf extract and the honeysuckle extract on the inhibition of propionibacterium acnes, the inhibition of the release of outer membrane vesicles of propionibacterium acnes and the anti-inflammatory effect is discussed, and the conditions and the results are shown in table 6.
TABLE 6
As can be seen from table 6, in the same case of experiment 2 in which the total concentration was 40 μ g/m L, the cytotoxicity was the least and the inhibition ratio of I L-8 was the best, i.e., the anti-inflammatory effect was the best, when the radix angelicae sinensis extract or the folium mori extract was added to the formulation, the cytotoxicity was greatly increased and the anti-inflammatory effect was deteriorated, compared to the case where the weight ratio of the radix sophorae flavescentis extract to the folium artemisiae argyi extract to the flos lonicerae extract was 1: 2: 1.
Example 7
The application of the acne-removing traditional Chinese medicine formula is shown in table 7.
TABLE 7
The preparation method of the cosmetic comprises the following steps: mixing and heating the phase A to 75-85 ℃ to be completely dissolved, mixing and heating the phase B to 40-50 ℃, adding the completely dissolved phase B into the phase A at 75-85 ℃ and uniformly stirring, cooling to below 40 ℃ after uniformly mixing the A, B phases, adding the phase C and uniformly stirring, and preparing the cosmetic.
The formula is verified to meet the pear flower index evaluation of cosmetics in cold resistance, heat resistance, pH value, viscosity, centrifugal test and the like; and the stability tests such as 7-cycle hot-cold circulation, appearance, physical and chemical indexes, active ingredients, microstructure and the like are performed, and the sanitation index evaluation such as corrosion resistance challenge, mercury, arsenic and lead content test and the like is simultaneously met.
As can be seen from Table 1, the composition (the traditional Chinese medicine extract with the multi-target acne removing effect prepared in example 3) is used in the formula at a concentration of 20-50 μ g/m L, and the addition amount of the composition accounts for 3% of the weight of the cosmetic, if the concentration of the composition in the formula exceeds 50 μ g/m L, certain toxicity is shown in a cell experiment, and certain safety risk exists, and when the concentration of the composition in the formula is lower than 20 μ g/m L, the acne inflammation inhibition effect is poor.
The invention discloses a composition with multi-target-point acne removing effect and a preparation method and application thereof. The inhibition of the formula on the propionibacterium acnes reproduction is embodied in a multi-target form, and the formula can ensure the efficacy of inhibiting acne inflammation and the safety. The sophora flavescens, the folium artemisiae argyi and the honeysuckle extracts in the formula can inhibit the reproduction of propionibacterium acnes and can also inhibit propionibacterium acnes from releasing outer membrane vesicles, and the effect of inhibiting acne inflammation is achieved.
Meanwhile, the inventor finds that when one traditional Chinese medicine extract is singly used in the sophora flavescens extract, the artemisia leaf extract and the honeysuckle extract, cytotoxicity is generated at a lower concentration, skin irritation is generated, and practical application is difficult. The traditional Chinese medicine extract has no additive effect on cytotoxicity, and the compound of the radix sophorae flavescentis extract, the folium artemisiae argyi extract and the honeysuckle extract can reduce cytotoxicity, has the effects of inhibiting propionibacterium acnes reproduction and inhibiting bacteria from releasing outer membrane vesicles, and can effectively inhibit acne inflammation.
It should be noted that the above-mentioned embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (10)
1. A composition with multi-target acne removing efficacy is characterized in that: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
the traditional Chinese medicine composition comprises 25-50% of radix sophorae flavescentis extract, 25-50% of folium artemisiae argyi extract and 10-25% of honeysuckle extract in percentage by mass of the raw materials.
2. The composition with the multi-target acne removing effect according to claim 1, wherein the preparation method of the sophora flavescens extract comprises the steps of drying sophora flavescens to constant weight, crushing, sieving by a 60-mesh sieve, carrying out reflux extraction for 1-2 hours by using an 80-100% ethanol solution according to a material-liquid ratio of g: m L to obtain a powder, continuously stirring, filtering after the first extraction is finished, extracting residues by using 80-100% ethanol for one time, filtering, combining two filtrates, carrying out rotary evaporation and concentration on the filtrates to obtain an extract, and carrying out freeze drying to obtain the sophora flavescens extract.
3. The composition with the multi-target acne removing effect according to claim 1, wherein the preparation method of the folium artemisiae argyi extract comprises the steps of drying folium artemisiae argyi to constant weight, crushing, sieving by a 60-mesh sieve, performing reflux extraction for 1-2 hours by using 60-80% ethanol solution according to the material-liquid ratio of g: m L to be 1: 6-8, performing continuous stirring, filtering after the first extraction is finished, extracting residues by using 60-80% ethanol for one time, filtering, combining the two filtrates, performing rotary evaporation and concentration on the filtrates to obtain an extract, and performing freeze drying to obtain the folium artemisiae argyi extract.
4. The composition with the multi-target acne removing effect according to claim 1, wherein the honeysuckle extract is prepared by drying honeysuckle to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 2-3 hours by using a 60-80% ethanol solution according to a material-liquid ratio of 1: 5-8 in terms of g: m L, continuously stirring, filtering after the first extraction is finished, extracting residues once by using 60-80% ethanol, filtering, combining two filtrates, performing rotary evaporation and concentration on the filtrates to obtain an extract, and performing freeze drying to obtain the honeysuckle extract.
5. The composition with multi-target acne removing efficacy of claim 1, wherein: the composition with the multi-target acne removing effect comprises 25% of the sophora flavescens extract, 50% of the folium artemisiae argyi extract and 25% of the honeysuckle extract in percentage by mass of the raw materials.
6. A method for preparing the composition with multi-target acne removing efficacy according to any one of claims 1 to 5, wherein the composition comprises the following components in percentage by weight: comprises the steps of (a) preparing a mixture of a plurality of raw materials,
drying radix sophorae flavescentis to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 1: 8-10 in terms of g: m L according to a material-liquid ratio, performing reflux extraction for 1-2 h by using 80-100% ethanol solution, stirring continuously, filtering after the first extraction is finished, extracting residues once by using 80-100% ethanol, filtering, combining filtrates obtained in two times, performing rotary evaporation and concentration on the filtrate to obtain an extract, and performing freeze drying to obtain the radix sophorae flavescentis extract;
the preparation method comprises the steps of drying folium artemisiae argyi to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 1: 6-8 by using 60-80% ethanol solution according to the material-liquid ratio (g: m L), stirring continuously, filtering after the first extraction is finished, extracting residues once by using 60-80% ethanol, filtering, combining the two filtrates, performing rotary evaporation and concentration on the filtrate to obtain extract, and performing freeze drying to obtain the folium artemisiae argyi extract;
the honeysuckle extract is prepared by drying honeysuckle to constant weight, crushing, sieving with a 60-mesh sieve, performing reflux extraction for 2-3 h by using 60-80% ethanol solution according to the material-liquid ratio of m L being 1: 5-8, stirring continuously, filtering after the first extraction, extracting residues once by using 60-80% ethanol, filtering, combining the two filtrates, performing rotary evaporation and concentration on the filtrate to obtain an extract, and performing freeze drying to obtain the honeysuckle extract;
preparation of the composition: according to the mass ratio percentage of each extract, 25-50% of radix sophorae flavescentis extract, 25-50% of folium artemisiae argyi extract and 10-25% of honeysuckle extract are mixed to obtain the composition with the multi-target acne removing effect.
7. The method for preparing the composition with multi-target acne removing efficacy according to claim 6, wherein the method comprises the following steps: the composition with the multi-target acne removing effect comprises 25% of radix sophorae flavescentis extract, 50% of folium artemisiae argyi extract and 25% of honeysuckle extract in percentage by mass.
8. Use of the composition with multi-target acne removing efficacy according to claim 6 in cosmetics.
9. The application of the composition with the multi-target acne removing effect in cosmetics is characterized in that the composition with the multi-target acne removing effect is 25-50% of radix sophorae flavescentis extract, 25-50% of folium artemisiae argyi extract and 10-25% of honeysuckle extract in percentage by mass, and the safe and effective concentration range in the formula is 20-50 μ g/m L.
10. The application of the composition with the multi-target acne removing effect in cosmetics is characterized in that the composition with the multi-target acne removing effect is composed of 25% of radix sophorae flavescentis extract, 50% of folium artemisiae argyi extract and 25% of honeysuckle extract in percentage by mass, and the safe and effective concentration range of the composition in a formula is 50 μ g/m L.
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