CN111449084A - Preparation for killing and inhibiting bacteria and viruses and use method thereof - Google Patents
Preparation for killing and inhibiting bacteria and viruses and use method thereof Download PDFInfo
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- CN111449084A CN111449084A CN202010385719.7A CN202010385719A CN111449084A CN 111449084 A CN111449084 A CN 111449084A CN 202010385719 A CN202010385719 A CN 202010385719A CN 111449084 A CN111449084 A CN 111449084A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
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Abstract
The invention belongs to the field of bactericides and provides a preparation for killing and inhibiting bacteria and viruses and a using method thereof, calcium hydroxide particles with the particle size of 0.01-10 mu m are dispersed in ethanol to form calcium hydroxide ethanol dispersion liquid, namely a disinfectant, the calcium hydroxide particles are micron-sized to nano-sized, have small particles and good dispersion degree, can permeate into pores in common decoration materials such as ceramic tiles, mortar joints, marble and the like, and are easy to permeate into wood by taking ethanol as a carrier. Ethanol has bactericidal effect, and calcium hydroxide with strong basicity reacts with CO in air2Before reaction to form calcium carbonateThe composition also has the effect of inhibiting the activity of bacteria and viruses, and can effectively inhibit the bacteria and the viruses particularly in a low-temperature and humid environment. Compared with the existing sterilization and disinfection technology, the invention has the advantages of high efficiency, long-acting effect, ecological environmental protection, one-time killing and long-acting inhibiting functions, and has important market value and social value.
Description
Technical Field
The invention belongs to the field of bactericides, and particularly relates to a preparation for killing and inhibiting bacteria and viruses and a using method thereof.
Background
Currently known sterilization methods are mainly classified into two types, one is a physical method such as killing viruses by using ultraviolet rays. The method is efficient and safe, but can harm people and plants due to improper treatment. The second method is a chemical method. Unlike other viruses, coronaviruses exhibit significant environmental resistance. Their survival in different biological fluids such as respiratory secretions or feces has been demonstrated. In addition, some parameters, such as low temperature and high relative humidity, also appear to be beneficial for coronavirus stabilization. When a disinfection strategy is formulated, a higher amount and/or concentration of disinfectant is often required, and the disinfectant has better killing effect and higher toxicity to people and environment. Therefore, an effective disinfection process should include a complete cleaning step, including a one-time kill and longer inhibition, while not being environmentally harmful. There are many chemical agents that can kill coronaviruses at one time, the most common being alcohol. Studies have shown that 70-80% ethanol kills coronavirus in 30 seconds to 2 minutes. Other disinfectants, such as some chlorine-containing disinfectants, have good effects of killing viruses and bacteria, and 0.1% sodium hypochlorite shows a more rapid effect, and can completely kill the coronavirus after acting for 1 minute. However, chlorine released in the process of acting can seriously stimulate the respiratory tract of people and pollute the environment such as soil, underground water and the like. Studies have shown that the activity of coronaviruses and other bacteria, mosses, etc. is sensitive to changes in pH in addition to temperature and humidity.
The most environmentally friendly alkaline material of nature is lime, or precisely the main component of lime calcium hydroxide (Ca (OH)2)。Ca(OH)2The pH value of the aqueous solution can reach 13-14, and the aqueous solution is strong alkaline. In this context, any organism, including viruses, cannot survive. However, Ca (OH) carried by water2The concentration is low, the coarse particles are 10-30 microns, the permeability is poor, and the treated surface is easily whitened, so that the application range of the treated surface is influenced.
Disclosure of Invention
The present invention has been made to solve the above problems, and an object of the present invention is to provide a preparation for killing and inhibiting bacteria and viruses, and a method of use thereof.
The invention provides a preparation for killing and inhibiting bacteria and viruses, which has the characteristics that: ethanol and calcium hydroxide, wherein the particle size of the calcium hydroxide is 0.01-10 mu m, and the calcium hydroxide is dispersed in the ethanol to form calcium hydroxide ethanol dispersion liquid.
The preparation for killing and inhibiting bacteria and viruses provided by the invention can also have the following characteristics: wherein the content of calcium hydroxide in the calcium hydroxide ethanol dispersion liquid is 10g Ca (OH)2/1000ml C2H5OH～70g Ca(OH)2/1000ml C2H5OH。
The invention also provides a use method of the preparation for killing and inhibiting bacteria and viruses, which is characterized by comprising the following steps: step 1, spraying 75% ethanol on the surface of an article to be disinfected for sterilization; step 2, after the surface of the object to be disinfected is dried, spraying a calcium hydroxide ethanol dispersion liquid with a first content; and 3, spraying the calcium hydroxide ethanol dispersion liquid with the second content after the surface of the object to be disinfected is dried.
In the method for using the preparation for killing and inhibiting bacteria and viruses, the preparation can also have the following characteristics: wherein the calcium hydroxide ethanol dispersion liquid comprises ethanol and calcium hydroxide, and the particle size of the calcium hydroxide is 0.01-10 μm.
In the method for using the preparation for killing and inhibiting bacteria and viruses, the preparation can also have the following characteristics: wherein the content of calcium hydroxide in the calcium hydroxide ethanol dispersion liquid is 10g Ca (OH)2/1000ml C2H5OH～70g Ca(OH)2/1000ml C2H5OH。
In the method for using the preparation for killing and inhibiting bacteria and viruses, the preparation can also have the following characteristics: wherein the content of calcium hydroxide in the first content of calcium hydroxide ethanol dispersion liquid is less than or equal to the content of calcium hydroxide in the second content of calcium hydroxide ethanol dispersion liquid.
Action and Effect of the invention
The invention provides a method for using calcium hydroxide ethanol dispersion liquid as disinfectantA method for disinfecting and sterilizing mosaic wall surface by using a disinfectant comprises the steps of dispersing calcium hydroxide particles with the particle size of 0.01-10 mu m in ethanol to form calcium hydroxide ethanol dispersion liquid, namely the disinfectant, spraying 75% ethanol on a to-be-disinfected object for disinfection, and then spraying the disinfectant for disinfection. The calcium hydroxide particles adopted by the method are micron-sized to nano-sized, small in particle size and good in dispersity, can permeate into pores in common decoration materials such as ceramic tiles, mortar joints, marble, granite and the like, and are easy to permeate into wood by taking ethanol as a carrier. Ethanol has bactericidal effect, and calcium hydroxide with strong basicity reacts with CO in air2Before the reaction to generate calcium carbonate, the calcium carbonate also has the effect of inhibiting the activity of bacteria and viruses, and can effectively inhibit the bacteria and the viruses particularly in a low-temperature and humid environment. Experiments prove that the surface treated by the bactericide is still alkaline within more than 7 days, which indicates that the bactericide still has the function of inhibiting the survival of bacteria and viruses.
Therefore, compared with the existing sterilization and disinfection technology, the invention has the advantages of high efficiency, long-acting effect, ecological environmental protection, one-time killing and long-acting inhibition functions, and has important market value and social value.
Drawings
FIG. 1 is a graph showing the actual effects of example 5 of the present invention and comparative example 2 after 30 days.
Detailed Description
In order to make the technical means, creation features, achievement objects and effects of the present invention easy to understand, the following embodiments and drawings are combined to describe the preparation for killing and inhibiting bacteria and viruses and the using method thereof.
The reagents and raw materials used in the examples of the present invention, comparative examples and test examples were purchased from general commercial sources.
The application method for killing and inhibiting bacteria and viruses provided by the invention specifically comprises the following steps:
step 1, spraying 75% ethanol to the articles to be sterilized for sterilization.
And 2, spraying the calcium hydroxide ethanol dispersion liquid with the first content after the surface of the object to be disinfected is dried.
And 3, spraying the calcium hydroxide ethanol dispersion liquid with the second content after the surface of the object to be disinfected is dried.
The calcium hydroxide ethanol dispersion liquid is formed by dispersing calcium hydroxide particles with the particle size of 0.01-10 mu m in ethanol.
The content of calcium hydroxide in the calcium hydroxide ethanol dispersion was 10g of Ca (OH)2/1000ml C2H5OH～70g Ca(OH)2/1000ml C2H5OH。
The content of calcium hydroxide in the first content of calcium hydroxide ethanol dispersion liquid is less than or equal to the content of calcium hydroxide in the second content of calcium hydroxide ethanol dispersion liquid.
The embodiments, the comparative examples and the test examples of the invention use the mosaic wall surface as the article to be disinfected, and also use the granite wall surface as the article to be disinfected. The mosaic wall surface is a mosaic wall surface between sanitations for 4 years, and dirt, mould and the like can be seen by naked eyes. The granite wall surface is an outdoor wall surface full of green moss.
< example 1>
In this embodiment, the calcium hydroxide ethanol dispersion is used as a disinfectant to sterilize and disinfect the mosaic wall surface, and the specific operations are as follows:
step 1, spraying 75% ethanol on the mosaic wall surface for sterilization;
step 2, after the ethanol is volatilized and the surface of the mosaic wall surface is dried, spraying Ca (OH) with the content of 10g of calcium hydroxide2/1000ml C2H5OH in calcium hydroxide ethanol dispersion;
step 3, after the surface of the mosaic wall surface is dried, spraying calcium hydroxide with the content of 10g Ca (OH)2/1000ml C2H5OH in calcium hydroxide ethanol dispersion.
< example 2>
In this embodiment, the calcium hydroxide ethanol dispersion is used as a disinfectant to sterilize and disinfect the mosaic wall surface, and the specific operations are as follows:
step 1, spraying 75% ethanol on the mosaic wall surface for sterilization;
step 2, after the ethanol is volatilized and the surface of the mosaic wall surface is dried, spraying Ca (OH) with the content of 30g of calcium hydroxide2/1000ml C2H5OH in calcium hydroxide ethanol dispersion;
step 3, after the surface of the mosaic wall surface is dried, spraying calcium hydroxide with the content of 30g Ca (OH)2/1000ml C2H5OH in calcium hydroxide ethanol dispersion.
< example 3>
In this embodiment, the calcium hydroxide ethanol dispersion is used as a disinfectant to sterilize and disinfect the mosaic wall surface, and the specific operations are as follows:
step 1, spraying 75% ethanol on the mosaic wall surface for sterilization;
step 2, after the ethanol is volatilized and the surface of the mosaic wall surface is dried, spraying Ca (OH) with the content of 30g of calcium hydroxide2/1000ml C2H5OH in calcium hydroxide ethanol dispersion;
step 3, after the surface of the mosaic wall surface is dried, spraying calcium hydroxide with the content of 50g Ca (OH)2/1000ml C2H5OH in calcium hydroxide ethanol dispersion.
< example 4>
In this embodiment, the calcium hydroxide ethanol dispersion is used as a disinfectant to sterilize and disinfect the mosaic wall surface, and the specific operations are as follows:
step 1, spraying 75% ethanol on the mosaic wall surface for sterilization;
step 2, after the ethanol is volatilized and the surface of the mosaic wall surface is dried, spraying Ca (OH) with the content of 30g of calcium hydroxide2/1000ml C2H5OH in calcium hydroxide ethanol dispersion;
step 3, after the surface of the mosaic wall surface is dried, spraying calcium hydroxide with the content of 70g Ca (OH)2/1000ml C2H5OH in calcium hydroxide ethanol dispersion.
< example 5>
In the embodiment, the moss killing of the granite outer wall surface full of moss by using the calcium hydroxide ethanol dispersion as a disinfectant comprises the following specific operations:
step 1, spraying 75% ethanol on the granite wall surface for sterilization;
step 2, after the ethanol is volatilized and the surface of the granite wall surface is dried, spraying Ca (OH) with the content of 30g of calcium hydroxide2/1000ml C2H5OH in calcium hydroxide ethanol dispersion;
step 3, after the surface of the mosaic wall surface is dried, spraying calcium hydroxide with the content of 30g Ca (OH)2/1000ml C2H5OH in calcium hydroxide ethanol dispersion.
< comparative example 1>
The mosaic wall surface is sprayed by using common commercially available disinfecting alcohol, namely 75% ethanol, as a disinfectant.
And (3) effect inspection: spraying the disinfectant alcohol for 15 minutes, and then spraying phenolphthalein with the mass concentration of 1% onto the mosaic wall surface, wherein the surface has no color change (the pH value is less than or equal to 8.0), which indicates that the 75% disinfectant alcohol has no function of inhibiting bacteria.
< comparative example 2>
The comparative example only uses the calcium hydroxide ethanol dispersion as the disinfectant to kill the moss on the granite outer wall, and the specific operation is as follows:
the content of calcium hydroxide sprayed once was 30g Ca (OH)2/1000ml C2H5OH-calcium hydroxide ethanol dispersion.
< test example >
In the test example, the mosaic walls of examples 1 to 4 and comparative example 1 were in an indoor environment where the temperature was 15 ℃ to 20 ℃ and the relative air humidity was 40% to 75%. The granite walls of example 5 and comparative example 2 were exposed to an outdoor environment at 10 ℃ to 26 ℃ and a relative air humidity of 30% to 100%.
After spraying the disinfectant for 15min, compared with the mosaic wall surfaces before spraying, the surfaces of the mosaic wall surfaces in the embodiments 1 to 3 have no obvious change, and the surfaces of the mosaic wall surfaces in the embodiment 4 have very slight whitening.
After the disinfectant is sprayed on the mosaic wall surface for 15min, the surface of the mosaic wall surface in examples 1 to 4 is obviously discolored (pH is not less than 8.0) by spraying the phenolphthalein with the mass concentration of 1%, which indicates that the mosaic wall surface has the effect of inhibiting viruses. After 7 days, the surfaces of the examples 1 to 4 are obviously discolored after the phenolphthalein with the concentration of 1% is sprayed, but the phenolphthalein is sprayed again after 10 days, and the examples 1 and 2 are not discolored, which shows that the effects of the phenolphthalein can last for 7 days in an indoor environment with the temperature of 15-20 ℃ and the relative air humidity of 40-75%. The surface of the mosaic wall surface in example 2 was significantly discolored (pH ≧ 8.0) after spraying phenolphthalein with a concentration of 1% after 15 minutes, indicating that the mosaic wall surface had a virus-inhibiting effect. After 7 days, the surface is obviously discolored after the phenolphthalein with the concentration of 1% is sprayed, but no discoloration is seen after the phenolphthalein is sprayed for 10 days, which indicates that the effect can last for 7 days in an indoor environment with the temperature of 15-20 ℃ and the relative air humidity of 40-75%.
The surfaces of the mosaic wall surfaces of examples 3 and 4 were significantly discolored (pH ≧ 8.0) after 10 days when phenolphthalein was sprayed at a concentration of 1%, indicating that they had a virus-inhibitory effect. However, phenolphthalein was sprayed after 14 days, and no discoloration was observed in example 3, indicating that example 3 could last for more than 7 days, but less than 14 days, in an indoor environment at a temperature of 15 ℃ to 20 ℃ and a relative air humidity of 40% to 75%.
After 14 days, the surface of the mosaic wall surface in example 4 was sprayed with phenolphthalein at a concentration of 1%, and the surface was still discolored (pH ≧ 8.0), indicating that the mosaic wall surface had a virus-inhibiting effect. The effect can last for more than 14 days in an indoor environment with the temperature of 15-20 ℃ and the relative air humidity of 40-75 percent.
It can be seen from examples 1 to 4 and comparative example 1 that the calcium hydroxide ethanol dispersion has a good bactericidal effect after spraying 75% ethanol first and then spraying twice. And the higher the content of the calcium hydroxide is, the more the strong alkalinity is kept on the surface of the article for a long time, and the bacterial growth is inhibited. For example, in examples 1 and 2, a lower Ca (OH) content of 10g is used2/1000mlC2H5OH and 30g Ca (OH)2/1000ml C2H5The alkaline property of the surface of the object can be maintained for 7 days, which is less than 10 days; while example 3 used a higher content of 50g Ca (OH)2/1000ml C2H5The alkaline property of the surface of the object can be maintained for 10 days and less than 14 days by using OH calcium hydroxide ethanol dispersion liquid. Example 4 uses a higher content of 70g Ca (OH)2/1000ml C2H5OH, the alkaline property of the surface of the article can be maintained for more than 14 days.
FIG. 1 is a graph showing the actual effects of example 5 of the present invention and comparative example 2 after 30 days.
As shown in FIG. 1, the leftmost part of the wall surface before treatment was covered with green moss. The middle part is the result of the treatment of example 5, and the treated wall surface remained clean after 30 days without new moss growth. The rightmost part is the result of the treatment of comparative example 2, and the moss still appears on the treated wall surface 30 days later.
Effects and effects of the embodiments
According to the method for sterilizing and disinfecting the mosaic wall surface by using the calcium hydroxide ethanol dispersion liquid as the disinfectant, calcium hydroxide particles with the particle size of 0.01-10 mu m are dispersed in ethanol to form the calcium hydroxide ethanol dispersion liquid, namely the disinfectant, and then 75% ethanol is sprayed on an article to be disinfected for sterilization, and then the disinfectant is sprayed for disinfection. The calcium hydroxide particles adopted by the method are micron-sized to nano-sized, small in particle size and good in dispersity, can permeate into pores in common decoration materials such as ceramic tiles, mortar joints and marble, and are easy to permeate into wood by taking ethanol as a carrier. Ethanol has bactericidal effect, and calcium hydroxide with strong basicity reacts with CO in air2Before the reaction to generate calcium carbonate, the calcium carbonate also has the effect of inhibiting the activity of bacteria and viruses, and can effectively inhibit the bacteria and the viruses particularly in a low-temperature and humid environment. Experiments prove that the surface treated by the bactericide is still alkaline within more than 7 days, which indicates that the bactericide still has the function of inhibiting bacteriaThe function of virus survival.
In addition, the content of calcium hydroxide is 10g Ca (OH)2/1000ml C2H5OH～70g Ca(OH)2/1000ml C2H5OH, because low concentrations do not have long-term efficacy, and high concentrations can cause whitening of the treated surface and interfere with the use of the article.
In addition, the calcium hydroxide ethanol dispersion liquid with different contents is sprayed twice, and the calcium hydroxide ethanol dispersion liquid with low content is sprayed firstly, so that the calcium hydroxide can be favorably permeated into the article under the action of the ethanol with higher content; and then high-content calcium hydroxide ethanol dispersion liquid is sprayed, and the high-content calcium hydroxide is beneficial to keeping the alkalinity of the surface of the article for a long time and improving the long-acting property of sterilization and disinfection.
Therefore, the embodiment of the invention uses harmful substances such as chlorine-free substances, and has the advantages of high efficiency, long acting, ecological environmental protection, one-time killing and long-acting inhibiting functions, and important market value and social value compared with the existing sterilization and disinfection technology.
The above embodiments are preferred examples of the present invention, and are not intended to limit the scope of the present invention.
Claims (6)
1. A formulation for killing and inhibiting bacteria and viruses, comprising:
ethanol and calcium hydroxide, and the mixture is added into the mixture,
wherein the particle size of the calcium hydroxide is 0.01-10 mu m, and the calcium hydroxide is dispersed in the ethanol to form calcium hydroxide ethanol dispersion liquid.
2. The bactericidal and virucidal agent of claim 1, wherein:
wherein the content of calcium hydroxide in the calcium hydroxide ethanol dispersion liquid is 10g of Ca (OH)2/1000ml C2H5OH～70g Ca(OH)2/1000ml C2H5OH。
3. The use method of the preparation for killing and inhibiting bacteria and viruses is characterized by comprising the following steps:
step 1, spraying 75% ethanol to an article to be disinfected for sterilization;
step 2, after the surface of the object to be disinfected is dried, spraying a calcium hydroxide ethanol dispersion liquid with a first content;
and 3, spraying a second content of calcium hydroxide ethanol dispersion liquid after the surface of the object to be disinfected is dried.
4. The method of using the bacteria and viruses killing and inhibiting agent according to claim 3, wherein:
wherein the calcium hydroxide ethanol dispersion liquid comprises ethanol and calcium hydroxide, and the particle size of the calcium hydroxide is 0.01-10 μm.
5. The method of using the bacteria and viruses killing and inhibiting agent according to claim 4, wherein:
wherein the content of calcium hydroxide in the calcium hydroxide ethanol dispersion liquid is 10g of Ca (OH)2/1000ml C2H5OH～70g Ca(OH)2/1000ml C2H5OH。
6. The method of using the bacteria and viruses killing and inhibiting agent according to claim 5, wherein:
wherein the content of the calcium hydroxide in the first content of calcium hydroxide ethanol dispersion liquid is less than or equal to the content of the calcium hydroxide in the second content of calcium hydroxide ethanol dispersion liquid.
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