CN111388513A

CN111388513A - Animal external antiparasitic composition and preparation method thereof

Info

Publication number: CN111388513A
Application number: CN202010289060.5A
Authority: CN
Inventors: 皮灿辉; 肖小平; 汪猜
Original assignee: Wuxi Paiwei Pharmaceutical Technology Co ltd
Current assignee: Wuxi Paiwei Pharmaceutical Technology Co ltd
Priority date: 2020-04-14
Filing date: 2020-04-14
Publication date: 2020-07-10

Abstract

The invention provides an animal external antiparasitic composition and a preparation method thereof, wherein the animal external antiparasitic composition comprises the following components in percentage by weight: 1-15% of fipronil, 0.5-5% of pyrethrin, 2-10% of cedar oil, 1-5% of insect growth regulator, 1-15% of crystallization inhibitor, 0.01-3% of antioxidant, 1-25% of main solvent, and the balance of cosolvent to 100%, and also comprises 0.2-10% of dispersant, 1-5% of transdermal penetration enhancer, 2-10% of cosolvent and other auxiliary agents. Fipronil and pyrethrin are matched and complemented, so that the insecticidal effect and the effect taking speed are increased; by adding the insect growth regulator, the invention has high-efficiency killing effect on adults, simultaneously increases the killing effect on ova or pupas and enlarges the killing range; the addition of cedar oil improves or covers the unpleasant smell of the medicine, increases the experience feeling, increases the parasite expelling effect and promotes the recovery of animal dermatitis, and the addition of antioxidant and other auxiliary agents increases the parasite expelling and killing effect and the stability of the medicine property of the invention.

Description

Animal external antiparasitic composition and preparation method thereof

Technical Field

The invention relates to the technical field of parasite medicines, and particularly relates to an animal external antiparasitic composition and a preparation method thereof.

Background

At present, the market of pets develops rapidly, and a plurality of families feed pets such as cats, dogs and the like, but the pets such as the cats, the dogs and the like are extremely easy to be invaded and infected by parasites, such as fleas, mites, ticks, lice and the like, the parasites not only can cause uneasiness and diseases of pets, but also can be intermediate hosts of pathogens, and various diseases which are commonly suffered by people and animals can be brought to people through pathogen infection and transmission media. There are numerous insecticides for treating parasites in the field, which vary in their effectiveness against particular parasites and their cost, such as fipronil, also known as fipronil, a pyrazole compound with insecticidal, acaricidal, nematicidal and arthropodicidal activity, which was discovered in 1987 by researchers of the company rona-planck, france, and which are used in pets, mainly in both drop and spray formulations, and which are stored in the sebaceous glands of the animals after application, acting systemically by spreading of the oil on the skin surface. The medicine can not enter the body and blood circulation of the cat and dog, and the liver and kidney functions of the cat and dog can not be damaged. The product is convenient to use, but has high price and slow effect, the medicine has little effect on worm eggs, and the phenomenon of relapse is easy to occur in a plurality of days after the medicine is used. In addition, the skin problem generally occurs in the parasitic diseases of pets, and the existing pesticide only has the insecticidal efficacy and cannot improve the skin problem, so that the animal external antiparasitic composition which has great effect on adults, larvae and ova, good insecticidal effect, mild skin and can avoid the ants and the insects is needed to be designed.

Disclosure of Invention

The invention aims to provide an animal external antiparasitic composition which has great effect on adults, larvae and ova, has good deinsectization effect and mild skin and can avoid the ants and the insects, and a preparation method thereof.

In order to achieve the purpose, the invention provides an anti-parasite composition for external use of animals, which comprises the following components in percentage by weight: 1-15% of fipronil, 0.5-5% of pyrethrin, 2-15% of cedar oil, 1-5% of insect growth agent, 1-15% of crystallization inhibitor, 0.01-3% of antioxidant, 1-25% of main solvent and the balance of cosolvent to 100%.

More preferably, the antioxidant is one or more of BHA, BHT, butylated hydroxytoluene, di-tert-butyl-p-cresol, clove oil and rosemary oil.

Further preferably, the animal external antiparasitic composition further comprises one or more of a dispersing agent, a transdermal penetration enhancer, a cosolvent and a flavoring agent.

Further preferably, the external antiparasitic composition for animals further comprises 0.2-10% of a dispersant, 1-5% of a transdermal penetration enhancer and 2-10% of a cosolvent.

Further preferably, the cosolvent is one or more selected from ethanol, isopropanol, methanol, fatty oil, propylene glycol, ethyl lactate, N-methyl pyrrolidone and dimethyl sulfoxide.

More preferably, the main solvent is one or more selected from the group consisting of ethyl acetate, acetone, acetonitrile, diethylene glycol dimethyl ether, dipropylene glycol n-butyl ether, ethylene glycol monoethyl ether, ethylene glycol monomethyl ether, dipropylene glycol monoethyl ether, diethylene glycol monobutyl ether, benzyl alcohol, glycerol, polyethylene glycol, and cyclohexanol.

Further preferably, the crystallization inhibitor is one or more selected from the group consisting of cationic, anionic, nonionic and amphoteric surfactants.

The invention also provides a preparation method of the animal external antiparasitic composition, which comprises the following steps:

(1) the following components are provided according to weight percentage: 1-15% of fipronil, 0.5-5% of pyrethrin, 2-15% of cedar oil, 1-5% of an insect growth agent, 1-15% of a crystallization inhibitor, 1-25% of a main solvent, 0.01-3% of an antioxidant, a proper amount of a cosolvent, and 3.3-30% of other assistants, wherein the other assistants are selected from one or more of a dispersing agent, a transdermal penetration enhancer and a cosolvent;

(2) adding a main solvent accounting for 1-25% of the production amount into a liquid preparation tank;

(3) adding the prepared insect growth agent accounting for 1-5% of the production amount and the antioxidant accounting for 0.005-0.2% of the production amount into a liquid preparation tank, uniformly stirring, continuously stirring, sequentially adding the crystallization inhibitor accounting for 1-15% of the production amount and the cedar oil accounting for 2-15% of the production amount, and fully stirring until the mixture is completely dissolved;

(4) continuously stirring, sequentially adding fipronil accounting for 1-15% of the production amount and pyrethrin accounting for 0.5-5% of the production amount, heating to 55-65 ℃, and stirring until the mixture is completely dissolved;

(5) adding all other auxiliary agents, stirring until the auxiliary agents are completely dissolved, adding a proper amount of cosolvent until the production capacity is reached, and stirring uniformly again.

The invention has the beneficial effects that: the animal external antiparasitic composition takes fenoxanil as a main active ingredient, and is matched and complemented with pyrethrin, so that the insecticidal speed is increased, the effect is quick and the insecticidal effect is good; in addition, by adding the insect growth regulator and controlling the weight percentage content, the invention has high-efficiency killing effect on adults, simultaneously increases the killing effect on ova or pupas and enlarges the killing range of the invention; by adding a certain weight percentage of cedar oil, the unpleasant smell of the medicine is improved or covered, the experience feeling is increased, the insect expelling effect is increased, and the recovery of animal dermatitis is promoted. In addition, by adding the antioxidant and other auxiliary agents and controlling the weight percentage content of each component, the parasite expelling and killing effect and the drug property stability of the invention are improved.

Detailed Description

The embodiments described below are only a part of the embodiments of the present invention, and not all of them. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention provides an anti-parasite composition for external use on animals, which comprises the following components: fipronil, pyrethrin, cedar oil, insect growth agent, crystallization inhibitor, antioxidant, primary solvent, and co-solvent.

Wherein the mass volume ratio of each component is as follows: fipronil 1-20%, which can be but not limited to 1%, 2%, 4%, 6%, 8%, 10%, 12%, 14%, 16%, 18%, or 20% etc.; 0.5-5% of pyrethrin, which can be but not limited to 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5% or 5% etc.; 2-15% of cedar oil, which can be but is not limited to 2%, 4%, 6%, 8%, 10%, 12%, 14% or 15% and the like; 1-5% of insect growth agent, which can be but is not limited to 1%, 2%, 3%, 4% or 5%; 1-15% of crystallization inhibitor, which can be but is not limited to 1%, 2%, 4%, 6%, 8%, 10%, 12%, 14% or 15% and the like; 0.01-3% of antioxidant, which can be but is not limited to 0.01%, 0.1%, 0.6%, 1.1%, 1.6%, 2.1%, 2.6% or 3%; 1-25% of a main solvent, which may be, but not limited to, 1%, 2%, 4%, 6%, 8%, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 25%, or the like; and the cosolvent to 100%.

Fipronil has strong lipophilicity, can diffuse to sebaceous glands of body surface hair follicles for storage, and thus has a long residual life, and fipronil mainly blocks the neural membrane Cl controlled by gamma-aminobutyric acid receptor^-Channels, induced Cl^-Flow, causing extreme excitation of the nervous system and death of the worm. The fipronil has the advantages of broad spectrum, high efficiency and the like, has no cross resistance with the existing insecticide, has better control effect on pests which have resistance or sensitivity to organophosphorus, organochlorine, carbamate, pyrethroid and other insecticides, but has the defect of slow effect taking, and does not greatly meet the requirements of users on the effect taking speed.

The pyrethrin can disturb the normal physiology of insect nerves, so that the insect nerves die from excitation, spasm to paralysis, has the characteristics of broad spectrum, high efficiency, quick action, short residual period and low toxicity, can complement the fipronil when being used with the fipronil, increases the insecticidal speed, and has quick response and good effect.

The insect growth regulator is selected from one or more of juvenile hormone analogues including pyriproxyfen, fenoxycarb and pyridaben, ecdysone analogues, chitin synthesis inhibitors or other insect growth regulators; the ecdysone analogue comprises food-inhibiting hydrazine and tebufenozide; the chitin synthesis inhibitor comprises cyromazine, buprofezin, diflubenzuron, chlorbenzuron, flufenoxuron, chlorfluazuron, hexaflumuron, triflumuron, teflubenzuron and the like; other insect growth regulators include azadirachtin and azadirachtin extracted from Meliaceae plants. The insect growth agent can inhibit the physiological development of insects, such as continuous molting, new epidermis formation, feeding inhibition and the like, hinder and interfere the normal development of insects, so that larvae which are not hatched or metamorphosis can not eclose into adults and die. The insect growth agent is used in combination with the fipronil and the pyrethrin, so that the effect of repelling and killing adults and larvae can be further improved, meanwhile, the egg killing effect is achieved, and the deinsectization effect is excellent.

The cedar oil has astringent and antibacterial properties, and can help eliminate scab, pus and some chronic diseases such as eczema and psoriasis, and is helpful for recovery of animal dermatitis; the cedar oil is also a good insect repellent, and can effectively repel insects and keep the insects away from animals; in addition, the cedar oil can emit sandalwood-like wood fragrance or light rosin flavor, has a certain soothing effect, can calm the nervous tension box anxiety state caused by parasites by means of the soothing effect of the cedar oil, can improve or cover the unpleasant smell of medicaments, and can increase experience.

The antioxidant is one or more of BHA, BHT, dibutyl hydroxy toluene, di-tert-butyl p-cresol, clove oil and rosemary oil, and can protect the fenoprofen and the pyrethrin from being oxidized when permeating into the insect fat layer and keep the drug effect.

The main solvent is one or more of ethyl acetate, acetone, acetonitrile, diethylene glycol dimethyl ether, dipropylene glycol n-butyl ether, ethylene glycol monoethyl ether, ethylene glycol monomethyl ether, dipropylene glycol monoethyl ether, diethylene glycol monobutyl ether, benzyl alcohol, glycerol, polyethylene glycol and cyclohexanol. The cosolvent is one or more of ethanol, isopropanol, methanol, fatty oil, propylene glycol and ethyl lactate. All provide a good systemic environment by better solubilization of the other components of the antiparasitic composition for external application to the animal.

The crystallization inhibitor is one or more of cationic, anionic, nonionic and amphoteric surfactants, can effectively inhibit crystallization, and prolong the retention time of the drug on the skin of animals, thereby better playing a role in repelling and killing. The cationic surfactant is one of dicetyl dimethyl olfactive and octylamine hydrochloride; the anionic surfactant is one of alkali metal stearate, sodium abietate, alkyl sulfate, sodium dodecyl benzene sulfonate and dioctyl sodium sulfosuccinate; the nonionic surfactant is one of polyoxyethylene sorbitan ester, isopropyl myristate, polyoxyethylated alkyl ether, polyethylene glycol stearate, polyoxyethylene castor oil derivative, polyglycerol ester, polyoxyethylated fatty alcohol, ethylene oxide and propylene oxide copolymer; the amphoteric surfactant may be one of a lauryl-substituted betaine compound, polyvinylpyrrolidone, polyvinyl alcohol, vinyl pyrrolidone vinyl acetate copolymer, and lecithin.

The animal topical antiparasitic composition may further comprise one or more of a dispersant, a transdermal penetration enhancer, a co-solvent, and a flavoring agent. Can comprise 0.2-10% of dispersing agent, 1-5% of transdermal penetration enhancer and 2-10% of cosolvent.

The dispersing agent can reduce the time and energy required by the dispersing process, stabilize the dispersed dispersion, prevent flocculation and sedimentation and shorten the dispersing time, thereby promoting the uniformity of each particle in the system, on one hand, the dispersing agent can avoid the partial unqualified drug effect condition caused by different drug effects due to uneven distribution of drugs among compositions in the same batch, on the other hand, the dispersing agent can be ensured to be uniformly dispersed on the surface of the animal skin, and the drug application effect is ensured. The transdermal penetration enhancer can be one or more of sulfoxide and its analogues, azone, organic acid and esters, alcohols, etc., and can promote transdermal absorption of medicine, so that the medicine can rapidly enter into body to produce effect. The cosolvent can increase the solubility of the drug in the solvent, is beneficial to the preparation of the preparation, and can also increase the stability of the preparation.

(1) the following components are provided according to weight percentage: 1-15% of fipronil, 0.5-5% of pyrethrin, 0.1-5% of cedar oil, 1-5% of an insect growth agent, 1-15% of a crystallization inhibitor, 0.005-0.2% of an antioxidant, 1-25% of a main solvent, a proper amount of a cosolvent and 3.3-30% of other additives, wherein the other additives are selected from one or more of a dispersing agent, a transdermal penetration enhancer, a cosolvent and a flavoring agent;

(3) adding the prepared insect growth agent accounting for 1-5% of the production amount and the antioxidant accounting for 0.01-3% of the production amount into a liquid preparation tank, uniformly stirring, continuously adding the crystallization inhibitor accounting for 1-15% of the production amount and the cedar oil accounting for 2-15% of the production amount through stirring, and fully stirring until the mixture is completely dissolved;

The animal external antiparasitic composition can be spray or drop, is used for repelling insects on the body surface of an animal, can effectively kill adults, larvae and eggs of the parasites, can effectively repel the insects, avoid the approach of foreign bodies, prevent parasite infection, and is helpful for the recovery of animal dermatitis and the soothing of mental stress and anxiety of the animal caused by the parasites.

The following are specific examples:

example 1

(1) Weighing the following raw materials in percentage by weight:

fipronil 12%, pyrethrin 4%, cedar oil 13%, fenoxycarb 3%, polyoxyethylene sorbitan ester 7%, BHA 2%, diethylene glycol monobutyl ether 20%, glycerol 6%, oleic acid 3%, propylene carbonate 5% and propylene glycol to 100%.

(2) All diethylene glycol monobutyl ether in the liquid preparation tank;

(3) adding the prepared fenoxycarb and BHA into a liquid preparation tank, uniformly stirring, continuously stirring, sequentially adding polyoxyethylene sorbitan ester and cedar oil, and fully stirring until the polyoxyethylene sorbitan ester and the cedar oil are completely dissolved;

(4) continuously stirring, sequentially adding fipronil and pyrethrin, heating to 60 deg.C, and stirring to dissolve completely;

(5) adding glycerol, oleic acid and propylene carbonate, stirring until the glycerol, the oleic acid and the propylene carbonate are completely dissolved, adding a proper amount of propylene glycol until the production amount is reached, and stirring uniformly again.

Example 2

(1) Weighing the following raw materials in percentage by weight:

fipronil 12%, pyrethrin 1%, cedar oil 13%, fenoxycarb 3%, polyoxyethylene sorbitan ester 7%, BHA 2%, diethylene glycol monobutyl ether 20%, glycerol 6%, oleic acid 3%, propylene carbonate 5% and propylene glycol to 100%.

(2) All diethylene glycol monobutyl ether in the liquid preparation tank;

Example 3

(1) Weighing the following raw materials in percentage by weight:

fipronil 12%, pyrethrin 4%, cedar oil 4%, fenoxycarb 3%, polyoxyethylene sorbitan ester 7%, BHA 2%, diethylene glycol monobutyl ether 20%, glycerol 6%, oleic acid 3%, propylene carbonate 5% and propylene glycol to 100%.

(2) All diethylene glycol monobutyl ether in the liquid preparation tank;

(5) adding glycerol, oleic acid and propylene carbonate, stirring until the mixture is completely dissolved, adding a proper amount of propylene glycol until the production amount is reached, and stirring uniformly again.

Example 4(1) the raw materials of the components are weighed according to the following weight percentages:

fipronil 12%, pyrethrin 4%, cedar oil 13%, fenoxycarb 1%, polyoxyethylene sorbitan ester 7%, BHA 2%, diethylene glycol monobutyl ether 20%, glycerol 6%, oleic acid 3%, propylene carbonate 5% and propylene glycol to 100%.

(2) All diethylene glycol monobutyl ether in the liquid preparation tank;

Example 5

(1) Weighing the following raw materials in percentage by weight:

fipronil 12%, pyrethrin 4%, cedar oil 13%, fenoxycarb 3%, polyoxyethylene sorbitan ester 7%, BHA 0.05%, diethylene glycol monobutyl ether 20%, glycerol 6%, oleic acid 3%, propylene carbonate 5% and propylene glycol make up to 100%.

(2) All diethylene glycol monobutyl ether in the liquid preparation tank;

Comparative example 1

This comparative example contained no pyrethrin, the remaining components were the same as in example 1, and was prepared in the same manner as in example 1.

Comparative example 2

This comparative example does not contain cedar oil, the remaining components are the same as in example 1, and the preparation method is the same as in example 1.

Comparative example 3

This comparative example does not contain fenoxycarb, the remaining components are the same as in example 1, and the preparation method is the same as in example 1.

Comparative example 4

This comparative example contained no BHA, the remaining components were the same as in example 1, and the preparation method was the same as in example 1.

Effect test

The canine farm selects 100 sick dogs diagnosed by veterinarian to have ectoparasites, and randomly divides the sick dogs into 10 groups, namely a test group 1, a test group 2, a test group 3, a test group 4, a test group 5, a control group 1, a control group 2, a control group 3, a control group 4 and a control group 5, and each group comprises 10 dogs. Test group 1 the insect repellent drop of example 1 was administered to a dog in an lml dose of 0kg body weight per l, and dropped between the bones of the shoulders and feet of the dog, and the treatment was performed 1 time to observe the healing. Test group 2 the insect repellent drops of example 2 were administered to a dog in an lml dose of 0kg body weight per l, and dropped between the bones of the shoulders and feet of the dog, and the treatment was performed 1 time to observe the healing. Test group 3 the insect repellent drop of example 3 was administered to a dog in an lml dose of 0kg body weight per l, and dropped between the bones of the shoulders and feet of the dog, and the treatment was performed 1 time to observe the healing. Test group 4 the insect repellent drops of example 4 were administered to a dog in an lml dose of 0kg body weight per l, and dropped between the bones of the shoulders and feet of the dog, and the treatment was performed 1 time to observe the healing. Test group 5 the insect repellent drop of example 5 was administered to a dog in an lml dose of 0kg body weight per l, and dropped between the bones of the shoulders and feet of the dog, and the treatment was performed 1 time to observe the healing. In control group 1, the anthelmintic drop of comparative example 1 was administered, lml was administered per l of 0kg body weight, and the control group was dropped between the bones of the shoulders and feet of dogs, and the treatment was performed 1 time to observe the healing. In control group 2, the anthelmintic drop of comparative example 2 was administered at an lml dose of 0kg per l of body weight, and dropped between the bones of the shoulders and feet of dogs for 1 application to observe the healing. In control group 3, the insect repellent drop of comparative example 3 was administered in an lml dose of 0kg per l of body weight, and the insect repellent was dropped between the bones of the shoulders and feet of dogs for 1 application to observe the healing. In control group 4, the anthelmintic drop of comparative example 4 was administered to dogs in an lml dose of 0kg per l of body weight, and the control group was dropped between the bones of the shoulders and feet of dogs for 1 application to observe the healing. In the control group 5, monopterus feloni drop was administered, lml was administered per l of 0kg body weight, and the drop was applied between the bones of the shoulders and feet of dogs for 1 time to observe the healing. The test lasted one month.

The results show that after the test group 1 dogs take the medicine, after 12 hours, 10 dogs with the disease have obviously improved symptoms or have reduced live insects, the number of scratching of the dogs is obviously reduced, the depilation stops, the skin color is changed from flushing to pink, and the inflammation symptoms are reduced. After 36 hours, the affected dogs were opened and no parasite excretions nor living organisms were found. After 2 days, the dermatitis of 10 dogs basically disappeared and new hair begins to grow without scratching. After 4 days, the skin color of 10 dogs turned to normal, no damage, no pruritus and inflammation phenomena, no peculiar pesticide smell in the pesticide application process and no live insects in 2 weeks.

After the test group 2 had the medicine, after 18 hours, the disease symptoms of 10 affected dogs were obviously improved, or the number of scratching itch of the affected dogs was obviously reduced, the depilation was stopped, the skin color was changed from flush to pink, and the inflammation symptoms were relieved. At 42 hours, the hair of the sick dog was opened, and neither parasite excreta nor parasite live bodies were found. After 2 days, the dermatitis of 10 dogs basically disappeared and new hair begins to grow without scratching. After 4 days, the skin color of 10 dogs turned to normal, no damage, no pruritus and inflammation phenomena, no peculiar pesticide smell in the pesticide application process and no live insects in 2 weeks.

After the test group 3 had the medicine, after 12 hours, the disease symptoms of 10 affected dogs were obviously improved, or the number of scratching times of the affected dogs was obviously reduced, and after 36 hours, the affected dogs opened the hair, and no parasite excreta or living parasites were found. After 4 days, the skin color turned from flush to pink and the inflammatory symptoms were reduced. After 6 days, the skin color of 10 dogs is basically normal, no damage, no pruritus or inflammation phenomenon exists, the pesticide odor exists in the pesticide applying process, and no live insects appear within 2 weeks.

After the test group 4, the disease symptoms of 10 affected dogs began to improve 20 hours later, the inflammation symptoms were reduced, and after 2 days, the affected dogs were opened, and no parasite excretions were found and sporadic parasite lives were found. After 3 days, no parasite was present, and dermatitis of 10 dogs was substantially disappeared and new hairs began to grow without scratching. After 6 days, the skin color of 10 dogs turns to normal, no damage, no pruritus and inflammation phenomena, no peculiar pesticide smell in the pesticide application process, and the number of worm eggs is reduced within 7 days, so that live worms appear.

After the test group 5 had been administered, the symptoms of the disease began to improve in 10 affected dogs after 20 hours, and after 2 days, the hairs of the affected dogs were opened, the excrements of the parasites were reduced, and the inflammatory symptoms were reduced with sporadic parasite lives. After 3 days, no parasite was present, and dermatitis of 10 dogs was substantially disappeared and new hairs began to grow without scratching. After 6 days, the skin color of 10 dogs turned to normal, no damage, no pruritus and inflammation phenomena, no peculiar pesticide smell in the pesticide application process, and no live insects in 10 days.

After the control group 1, the disease symptoms of 10 affected dogs began to improve after 1 day, and after 2 days, the hairs of the affected dogs were opened, the excretion of parasites was reduced, the living parasites were scattered, and the dermatitis of 10 dogs improved. After 4 days, the skin color of 10 dogs is improved, the pruritus and the inflammation are further reduced, after 4 days, the skin color of 10 dogs is changed to be normal, no damage, no pruritus and inflammation phenomena exist, no peculiar pesticide smell exists in the pesticide application process, and no live insects appear in 2 weeks.

After the control group 2 of the sick dogs are used, after 12 hours, the sick symptoms of 10 sick dogs are obviously improved, or live insects are reduced, the scratching frequency of the sick dogs is obviously reduced, after 36 hours, hairs of the sick dogs are opened, no parasite excrement is found, and no parasite living bodies are found. After 6 days, the skin color turned from flush to pink, and the symptoms of itching and inflammation were reduced. The pesticide has peculiar smell during the pesticide application process, and no live insects appear within 2 weeks.

After the control group 3 had been administered, the symptoms of inflammation began to improve in 10 affected dogs 20 hours later, and the symptoms of inflammation of the skin were reduced, and after 2 days, the hairs of the affected dogs were opened, and the excretion of parasites was significantly reduced, and the symptoms of inflammation were reduced with sporadic living parasites. After 3 days, no parasite was present, and dermatitis of 10 dogs was substantially disappeared and new hairs began to grow without scratching. After 6 days, the skin color of 10 dogs turns to normal, no damage, no pruritus and inflammation phenomena, no peculiar pesticide smell in the pesticide application process, obvious reduction of worm eggs within 7 days and live worms.

After the control group 4 had been administered, the symptoms of the disease began to improve in 10 affected dogs 24 hours later, and after 2 days, the hairs of the affected dogs were opened, the number of excrements of the parasites was slightly reduced, and the number of living parasites was slightly increased, and the inflammation symptoms were slightly reduced. After 3 days, the skin dermatitis of 10 dogs basically disappeared with sporadic parasites, and new hairs began to grow without scratching. After 7 days, the skin color of 10 dogs turned to normal, no damage, no pruritus and inflammation phenomena, no peculiar pesticide smell in the pesticide application process, and no live insects in 10 days.

After the control group 5 dogs were dosed, the symptoms of the disease began to improve in 10 dogs after 1 day, and after 2 days, the hairs of the disease dogs were opened, the excretion of parasites was slightly reduced, and the inflammation was slightly reduced due to a little more parasites. After 4 days, with sporadic parasite presence, the symptoms of itching and inflammation were reduced but continued. After 7 days, the skin color of 10 dogs is improved, the pruritus and the inflammation are further reduced, no live insects or sporadic live insect bodies are further reduced, the peculiar smell of the pesticide is obvious in the pesticide application process, the insect eggs are obviously reduced within 7 days, and more live insects appear.

The addition of the pyrethrin is known from the test group 1, the test group 2, the control group 1 and the control group 5, so that the low-level parasite expelling effect of the external antiparasitic composition for animals is obviously improved and the effect is quicker compared with the single fenpropathrin; according to the test group 1 and the test group 3, the control group 2 and the control group 5, the addition of the cedar oil is beneficial to the recovery of animal dermatitis, and can improve or cover the unpleasant smell of the medicine and increase the experience feeling; as can be seen from the test groups 1 and 4, the control group 3 and the control group 5, the addition of the fenoxycarb, namely the insect growth agent, can prevent live insects from appearing on the animals within a certain period of time, because the addition of the insect growth agent inhibits the normal physiological activity of eggs, so that the eggs cannot be hatched normally; the experimental group 1, the experimental group 5, the control group 4 and the control group 5 show that the drug effect of the preparations of the experimental group 5, the control group 4 and the control group 5 is lower than that of the experimental group 1, and further, the addition of the BHA and the antioxidant is beneficial to the stability of the drug effect, so that the drug effect of the preparation is longer, and the addition of the antioxidant is known.

While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the scope of the invention.

Claims

1. An anti-parasite composition for external use on animals, which is characterized in that the weight ratio of each component of the anti-parasite composition for animals is as follows:

2. the animal topical antiparasitic composition of claim 1, wherein the antioxidant is one or more of BHA, BHT, dibutylhydroxytoluene, di-tert-butyl-p-cresol, clove oil, rosemary oil.

3. The animal topical antiparasitic composition of claim 1, further comprising one or more of a dispersant, a transdermal penetration enhancer, a co-solvent, and a flavoring agent.

4. The animal ectoparasiticidal composition of claim 3 further comprising 0.2% to 10% of a dispersant, 1% to 5% of a transdermal penetration enhancer, and 2% to 10% of a co-solvent.

5. An antiparasitic composition for external application to an animal according to claim 1, wherein the co-solvent is selected from one or more of ethanol, isopropanol, methanol, fatty oils, propylene glycol, ethyl lactate, N-methyl pyrrolidone, dimethyl sulfoxide.

6. The animal external antiparasitic composition of claim 1, wherein the primary solvent is selected from one or more of ethyl acetate, acetone, acetonitrile, diethylene glycol dimethyl ether, dipropylene glycol n-butyl ether, ethylene glycol monoethyl ether, ethylene glycol monomethyl ether, dipropylene glycol monoethyl ether, diethylene glycol monobutyl ether, benzyl alcohol, glycerol, polyethylene glycol, and cyclohexanol.

7. An antiparasitic composition for external application to an animal according to claim 1, wherein the crystallization inhibitor is selected from one or more of the group consisting of cationic, anionic, nonionic and amphoteric surfactants.

8. A method for preparing an antiparasitic composition for external application to animals, according to any one of claims 1 to 7, comprising the following steps:

(1) the following components are provided according to weight percentage: 1-15% of fipronil, 0.5-5% of pyrethrin, 2-15% of cedar oil, 1-5% of an insect growth agent, 1-15% of a crystallization inhibitor, 1-25% of a main solvent, 0.01-3% of an antioxidant, a proper amount of a cosolvent, and 3.2-25% of other assistants, wherein the other assistants are selected from one or more of a dispersing agent, a transdermal penetration enhancer and a cosolvent;

(3) adding the prepared insect growth regulator accounting for 1-5% of the production amount and the antioxidant accounting for 0.005-0.2% of the production amount into a liquid preparation tank, uniformly stirring, continuously stirring, sequentially adding the crystallization inhibitor accounting for 1-15% of the production amount and the cedar oil accounting for 2-15% of the production amount, and fully stirring until the mixture is completely dissolved;