CN111387362A - Antibacterial growth-assisting animal feed prepared based on rice bran and preparation method thereof - Google Patents

Antibacterial growth-assisting animal feed prepared based on rice bran and preparation method thereof Download PDF

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Publication number
CN111387362A
CN111387362A CN202010385158.0A CN202010385158A CN111387362A CN 111387362 A CN111387362 A CN 111387362A CN 202010385158 A CN202010385158 A CN 202010385158A CN 111387362 A CN111387362 A CN 111387362A
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China
Prior art keywords
parts
rice bran
animal feed
antibacterial growth
growth
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CN202010385158.0A
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Chinese (zh)
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渠志灿
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Individual
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Priority to CN202010385158.0A priority Critical patent/CN111387362A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

Abstract

The invention discloses an antibacterial growth-assisting animal feed prepared based on rice bran and a preparation method thereof, and particularly relates to the field of animal feeds, wherein the antibacterial growth-assisting animal feed comprises the following components in parts by weight: 20-40 parts of rice bran, 10-15 parts of radix tetrastigme, 5-10 parts of fig, 8-15 parts of sansevieria trifasciata, 5-8 parts of ginkgo leaves, 3-6 parts of comfrey, 2-5 parts of sweet clover, 15-25 parts of carrot and 15-25 parts of corn. The antibacterial growth-assisting animal feed provided by the invention has the advantages of promoting the growth of animals and reducing the morbidity.

Description

Antibacterial growth-assisting animal feed prepared based on rice bran and preparation method thereof
Technical Field
The invention relates to the field of animal feed, in particular to an antibacterial growth-assisting animal feed prepared based on rice bran and a preparation method thereof.
Background
With the development of science and technology and the improvement of living standard, the breeding of fish, chicken and pig has gradually changed from the family type captive breeding to the large-scale breeding base. In the face of large-scale cultivation, animal feed undoubtedly becomes an important link,
at present, antibiotics are commonly added into pig feed in China, so that the pig feed is used for improving the survival rate of piglets and has the effect of promoting the growth of the piglets. However, the addition of antibiotics to feeds raises food safety problems such as drug resistance. Although the Ministry of agriculture in China has banned the application of various powerful antibiotics in the feed, once the season with many diseases comes, because bacteriostatic agents more effective than the antibiotics are difficult to find in the market at present, even if feed manufacturers do not add the antibiotics, the workers of the breeding plants can add the antibiotics into the feed to ensure the survival rate of piglets. Therefore, the development of efficient and safe feed mildew preventive and antibacterial growth promoter is the trend of the feed additive industry.
Disclosure of Invention
The invention provides an antibacterial growth-assisting animal feed prepared based on rice bran and a preparation method thereof.
In order to achieve the purpose, the invention provides the following technical scheme:
an antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 20-40 parts of rice bran, 10-15 parts of radix tetrastigme, 5-10 parts of fig, 8-15 parts of sansevieria trifasciata, 5-8 parts of ginkgo leaves, 3-6 parts of comfrey, 2-5 parts of sweet clover, 15-25 parts of carrot and 15-25 parts of corn.
Preferably, the antibacterial growth-promoting animal feed further comprises any one of broussonetia papyrifera, lotus leaves, pennisetum sinese and alfalfa, or a mixture of the two.
Preferably, the antibacterial growth-promoting animal feed further comprises at least one of distillers grains and bagasse.
Preferably, the antibacterial growth-promoting animal feed comprises the following components in parts by weight: 20-40 parts of rice bran, 10-15 parts of radix tetrastigme, 5-10 parts of fig, 8-15 parts of sansevieria trifasciata, 5-8 parts of ginkgo leaves, 3-6 parts of comfrey, 2-5 parts of sweet clover, 3-5 parts of broussonetia papyrifera fruits, 1-3 parts of lotus leaves, 10-20 parts of pennisetum hydridum, 10-20 parts of alfalfa, 15-25 parts of carrots, 15-25 parts of corn and 3-12 parts of vinasse.
Preferably, the antibacterial growth-promoting animal feed comprises the following components in parts by weight: 30 parts of rice bran, 12 parts of radix tetrastigme, 7 parts of fig, 10 parts of sansevieria trifasciata, 6 parts of ginkgo leaves, 4 parts of comfrey, 3 parts of sweet clover, 4 parts of broussonetia papyrifera, 2 parts of lotus leaves, 15 parts of pennisetum hydridum, 15 parts of alfalfa, 20 parts of carrots, 20 parts of corns and 7 parts of vinasse.
The invention also provides a preparation method of the antibacterial growth-assisting animal feed, which comprises the following steps:
s01, washing and drying Tetrastigma hemsleyanum Diels et Gilg, figs, sansevieria trifasciata, folium ginkgo, comfrey, sweet clover, broussonetia papyrifera fruits, lotus leaves, Jujuncao, alfalfa, carrots and corns in preset parts by weight, uniformly mixing, grinding, and sieving with a 30-50-mesh sieve to prepare the first mixed particle.
S02, taking rice bran in a preset weight part, cleaning, adding vinasse and purified water, stirring uniformly, heating at the temperature of 30-40 ℃ for 40-60 min, naturally drying, grinding, and sieving with a 30-50-mesh sieve to obtain second mixed particles.
S03, uniformly mixing the prepared first mixed particles and the second mixed particles to obtain the antibacterial growth-assisting animal feed.
Preferably, in step S01, the first mixed particle has a particle size of 40 mesh.
Preferably, in step S02, the heating temperature is 36 ℃ and the heating time is 50 min.
Preferably, in step S02, the second mixed particle has a particle size of 40 mesh.
Preferably, in step S03, the antibacterial growth-promoting animal feed has a water content of 1% or less.
The invention has the beneficial effects that:
according to the invention, by adding various plant-derived forage, coarse food grains and rice bran into the antibacterial growth-assisting animal feed, nutrients required by daily development of piglets can be supplemented, the infection rate of the piglets in the growth link can be reduced, and the immunity and anti-inflammatory capability of the piglets are improved. Meanwhile, after the pork is eaten by a human body, the possibility that antibiotics enter the human body through a food chain can not occur, and the safety of food sources is guaranteed.
Detailed Description
Example 1
An antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 20 parts of rice bran, 10 parts of radix tetrastigme, 5 parts of fig, 8 parts of sansevieria trifasciata, 5 parts of ginkgo leaves, 3 parts of comfrey, 2 parts of sweet clover, 3 parts of broussonetia papyrifera, 1 part of lotus leaves, 10 parts of pennisetum hydridum, 10 parts of alfalfa, 15 parts of carrots, 15 parts of corns and 3 parts of vinasse.
The preparation method of the antibacterial growth-assisting animal feed comprises the following steps:
s01, washing and drying Tetrastigma hemsleyanum Diels et Gilg, Ficus carica, sansevieria trifasciata, folium Ginkgo, comfrey, sweet clover, Broussonetia papyrifera fruit, lotus leaf, Jujuncao, alfalfa, carrot and corn in preset parts by weight, uniformly mixing, grinding, and sieving with a 40-mesh sieve to prepare the first mixed particle.
S02, taking rice bran in a preset weight part, cleaning, adding vinasse and purified water, stirring uniformly, heating at the temperature of 36 ℃ for 50min, naturally drying, grinding, and sieving with a 40-mesh sieve to prepare second mixed particles.
S03, uniformly mixing the prepared first mixed particles and the second mixed particles to obtain the antibacterial growth-assisting animal feed.
After the piglets eat the antibacterial growth-promoting animal feed for 6 months, the average weight is 120kg, the morbidity is 0, and the number of the bred piglets is 20.
Example 2
An antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 30 parts of rice bran, 12 parts of radix tetrastigme, 7 parts of fig, 10 parts of sansevieria trifasciata, 6 parts of ginkgo leaves, 4 parts of comfrey, 3 parts of sweet clover, 4 parts of broussonetia papyrifera, 2 parts of lotus leaves, 15 parts of pennisetum hydridum, 15 parts of alfalfa, 20 parts of carrots, 20 parts of corns and 7 parts of vinasse.
The preparation method of the antibacterial growth-promoting animal feed provided by the embodiment is the same as that of the animal feed provided by the embodiment 1.
After the piglets eat the antibacterial growth-promoting animal feed for 6 months, the average weight is 123kg, the morbidity is 0, and the number of the bred piglets is 20.
Example 3
An antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 35 parts of rice bran, 13 parts of radix tetrastigme, 8 parts of fig, 12 parts of sansevieria trifasciata, 7 parts of ginkgo leaves, 5 parts of comfrey, 4 parts of sweet clover, 4 parts of broussonetia papyrifera, 2 parts of lotus leaves, 17 parts of pennisetum hydridum, 18 parts of alfalfa, 23 parts of carrots, 23 parts of corns and 8 parts of vinasse.
The preparation method of the antibacterial growth-promoting animal feed provided by the embodiment is the same as that of the animal feed provided by the embodiment 1.
After the piglets eat the antibacterial growth-promoting animal feed for 6 months, the average weight is 118kg, the disease incidence number is 0, and the number of the bred piglets is 20.
Example 4
An antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 40 parts of rice bran, 15 parts of radix tetrastigme, 10 parts of fig, 15 parts of sansevieria trifasciata, 8 parts of ginkgo leaves, 6 parts of comfrey, 5 parts of sweet clover, 5 parts of broussonetia papyrifera, 3 parts of lotus leaves, 20 parts of pennisetum hydridum, 20 parts of alfalfa, 25 parts of carrots, 25 parts of corns and 12 parts of vinasse.
The preparation method of the antibacterial growth-promoting animal feed provided by the embodiment is the same as that of the animal feed provided by the embodiment 1.
After the piglets eat the antibacterial growth-promoting animal feed for 6 months, the average weight is 119kg, the disease number is 1, and the number of the bred piglets is 20.
Comparative example 1
An antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 12 parts of radix tetrastigme, 7 parts of fig, 10 parts of sansevieria trifasciata, 6 parts of ginkgo leaves, 4 parts of comfrey, 3 parts of sweet clover, 4 parts of broussonetia papyrifera fruits, 2 parts of lotus leaves, 15 parts of pennisetum hydridum, 15 parts of alfalfa, 20 parts of carrots, 20 parts of corns and 7 parts of vinasse.
The preparation method of the antibacterial growth-promoting animal feed provided by the comparative example is the same as that of example 1.
After the piglets eat the antibacterial growth-promoting animal feed for 6 months, the average weight is 110kg, the disease number is 2, and the number of the bred piglets is 20.
Comparative example 2
An antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 30 parts of rice bran, 10 parts of sansevieria trifasciata, 6 parts of ginkgo leaves, 4 parts of comfrey, 3 parts of sweet clover, 4 parts of broussonetia papyrifera fruits, 2 parts of lotus leaves, 15 parts of pennisetum sinese, 15 parts of alfalfa, 20 parts of carrots, 20 parts of corn and 7 parts of vinasse.
The preparation method of the antibacterial growth-promoting animal feed provided by the comparative example is the same as that of example 1.
After the piglets eat the antibacterial growth-promoting animal feed for 6 months, the average weight is 110kg, the disease number is 6, and the number of the bred piglets is 20.
Comparative example 3
An antibacterial growth-assisting animal feed prepared based on rice bran comprises the following components in parts by weight: 30 parts of rice bran, 12 parts of radix tetrastigme, 7 parts of fig, 10 parts of sansevieria trifasciata, 6 parts of ginkgo leaves, 4 parts of comfrey, 3 parts of sweet clover, 2 parts of lotus leaves, 15 parts of pennisetum hydridum, 15 parts of alfalfa, 20 parts of carrots and 20 parts of corns.
The preparation method of the antibacterial growth-promoting animal feed provided by the comparative example is the same as that of example 1.
After the piglets eat the antibacterial growth-promoting animal feed for 6 months, the average weight is 112kg, the disease number is 3, and the number of the bred piglets is 20.

Claims (10)

1. An antibacterial growth-assisting animal feed prepared based on rice bran is characterized by comprising the following components in parts by weight: 20-40 parts of rice bran, 10-15 parts of radix tetrastigme, 5-10 parts of fig, 8-15 parts of sansevieria trifasciata, 5-8 parts of ginkgo leaves, 3-6 parts of comfrey, 2-5 parts of sweet clover, 15-25 parts of carrot and 15-25 parts of corn.
2. The antibacterial growth-promoting animal feed prepared from rice bran according to claim 1, further comprising any one of broussonetia papyrifera, lotus leaf, pennisetum sinese and alfalfa, or a mixture thereof.
3. The antibacterial growth-promoting animal feed prepared from rice bran according to claim 1, further comprising at least one of distillers grains and bagasse.
4. The antibacterial growth-promoting animal feed prepared based on rice bran according to claim 1 is characterized by comprising the following components in parts by weight: 20-40 parts of rice bran, 10-15 parts of radix tetrastigme, 5-10 parts of fig, 8-15 parts of sansevieria trifasciata, 5-8 parts of ginkgo leaves, 3-6 parts of comfrey, 2-5 parts of sweet clover, 3-5 parts of broussonetia papyrifera fruits, 1-3 parts of lotus leaves, 10-20 parts of pennisetum hydridum, 10-20 parts of alfalfa, 15-25 parts of carrots, 15-25 parts of corn and 3-12 parts of vinasse.
5. The antibacterial growth-promoting animal feed prepared based on rice bran according to claim 1 is characterized by comprising the following components in parts by weight: 30 parts of rice bran, 12 parts of radix tetrastigme, 7 parts of fig, 10 parts of sansevieria trifasciata, 6 parts of ginkgo leaves, 4 parts of comfrey, 3 parts of sweet clover, 4 parts of broussonetia papyrifera, 2 parts of lotus leaves, 15 parts of pennisetum hydridum, 15 parts of alfalfa, 20 parts of carrots, 20 parts of corns and 7 parts of vinasse.
6. The preparation method of the antibacterial growth-assisting animal feed is characterized by comprising the following steps:
s01, taking tetrastigma hemsleyanum Diels et Gilg, figs, sansevieria trifasciata, ginkgo leaves, comfrey, sweet clover, broussonetia papyrifera fruits, lotus leaves, Jujuncao, alfalfa, carrots and corns in preset parts by weight, cleaning and drying the materials, uniformly mixing the materials, grinding the materials, and sieving the ground materials by a sieve of 30-50 meshes to prepare first mixed particles;
s02, taking rice bran in a preset weight part, cleaning, adding vinasse and purified water, stirring uniformly, heating at the temperature of 30-40 ℃ for 40-60 min, naturally drying, grinding, and sieving with a 30-50-mesh sieve to obtain second mixed particles;
s03, uniformly mixing the prepared first mixed particles and the second mixed particles to obtain the antibacterial growth-assisting animal feed.
7. The antibacterial growth promoting animal feed prepared from rice bran according to claim 6, wherein the first mixed particle has a particle size of 40 mesh in step S01.
8. The antibacterial growth-promoting animal feed prepared from rice bran according to claim 6, wherein the heating temperature is 36 ℃ and the heating time is 50min in step S02.
9. The antibacterial growth promoting animal feed prepared from rice bran according to claim 6, wherein the second mixed particle has a particle size of 40 mesh in step S02.
10. The feed for the antibacterial growth-promoting animal prepared from rice bran according to claim 6, wherein the water content of the feed for the antibacterial growth-promoting animal is less than or equal to 1% in step S03.
CN202010385158.0A 2020-05-09 2020-05-09 Antibacterial growth-assisting animal feed prepared based on rice bran and preparation method thereof Pending CN111387362A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104686848A (en) * 2015-03-13 2015-06-10 麻阳弘胜生态循环农业科技有限公司 Radix tetrastigme zinc and selenium enriched pig feed and production method thereof
CN106798117A (en) * 2016-11-28 2017-06-06 河池市金城江区科学技术情报研究所 A kind of pig starter feed of strengthen immunity and preparation method thereof
CN106858093A (en) * 2016-12-25 2017-06-20 吴变美 A kind of pig feed for promoting digestion
WO2018049657A1 (en) * 2016-09-19 2018-03-22 陈云 Healthy nutritious feed for animals
CN110710605A (en) * 2019-10-29 2020-01-21 广西壮族自治区百朋种畜场 Broussonetia papyrifera leaf fermented feed for improving quality of goat meat and preparation method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104686848A (en) * 2015-03-13 2015-06-10 麻阳弘胜生态循环农业科技有限公司 Radix tetrastigme zinc and selenium enriched pig feed and production method thereof
WO2018049657A1 (en) * 2016-09-19 2018-03-22 陈云 Healthy nutritious feed for animals
CN106798117A (en) * 2016-11-28 2017-06-06 河池市金城江区科学技术情报研究所 A kind of pig starter feed of strengthen immunity and preparation method thereof
CN106858093A (en) * 2016-12-25 2017-06-20 吴变美 A kind of pig feed for promoting digestion
CN110710605A (en) * 2019-10-29 2020-01-21 广西壮族自治区百朋种畜场 Broussonetia papyrifera leaf fermented feed for improving quality of goat meat and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
樊智芬: "紫花苜蓿饲料的制备及其在生猪养殖中的应用", 《当代畜牧》 *

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