CN111337662A - 一种基于微流控芯片的快速免疫检测方法 - Google Patents

一种基于微流控芯片的快速免疫检测方法 Download PDF

Info

Publication number
CN111337662A
CN111337662A CN202010116126.0A CN202010116126A CN111337662A CN 111337662 A CN111337662 A CN 111337662A CN 202010116126 A CN202010116126 A CN 202010116126A CN 111337662 A CN111337662 A CN 111337662A
Authority
CN
China
Prior art keywords
sample
detection
microfluidic chip
detected
immunocapture
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202010116126.0A
Other languages
English (en)
Chinese (zh)
Inventor
周建华
刘廙人
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sun Yat Sen University
Original Assignee
Sun Yat Sen University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sun Yat Sen University filed Critical Sun Yat Sen University
Priority to CN202010116126.0A priority Critical patent/CN111337662A/zh
Priority to PCT/CN2020/096717 priority patent/WO2021169089A1/fr
Publication of CN111337662A publication Critical patent/CN111337662A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306Solid-phase reaction mechanisms
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983Viruses
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854Immunoglobulins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/11Orthomyxoviridae, e.g. influenza virus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08RNA viruses
    • G01N2333/165Coronaviridae, e.g. avian infectious bronchitis virus
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2469/00Immunoassays for the detection of microorganisms
    • G01N2469/20Detection of antibodies in sample from host which are directed against antigens from microorganisms

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
CN202010116126.0A 2020-02-25 2020-02-25 一种基于微流控芯片的快速免疫检测方法 Pending CN111337662A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202010116126.0A CN111337662A (zh) 2020-02-25 2020-02-25 一种基于微流控芯片的快速免疫检测方法
PCT/CN2020/096717 WO2021169089A1 (fr) 2020-02-25 2020-06-18 Procédé de dosage immunologique rapide basé sur une puce microfluidique

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010116126.0A CN111337662A (zh) 2020-02-25 2020-02-25 一种基于微流控芯片的快速免疫检测方法

Publications (1)

Publication Number Publication Date
CN111337662A true CN111337662A (zh) 2020-06-26

Family

ID=71185498

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010116126.0A Pending CN111337662A (zh) 2020-02-25 2020-02-25 一种基于微流控芯片的快速免疫检测方法

Country Status (2)

Country Link
CN (1) CN111337662A (fr)
WO (1) WO2021169089A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111781379A (zh) * 2020-07-16 2020-10-16 天津中新科炬生物制药股份有限公司 一种用于新型冠状病毒联合检测的微流控芯片模组
CN112964865A (zh) * 2021-01-18 2021-06-15 中山大学 一种通道形貌控制流体混合的超快速免疫检测芯片及其制备方法和应用
WO2023284686A1 (fr) * 2021-07-12 2023-01-19 西湖大学 Procédé et système de détection par biodétection pour des anticorps de liaison
CN116679049A (zh) * 2023-02-27 2023-09-01 科赫生物科技(北京)有限公司 一种免疫检测方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115228517A (zh) * 2022-03-17 2022-10-25 烟台大学 一种基于框架核酸检测病毒的旋转型微流控纸芯片及其制备方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE387630T1 (de) * 2003-12-22 2008-03-15 Micro Typing Systems Inc Verkürzung der zeit bis zum vorliegen von blutbankdiagnostikergebnissen
CN101221167A (zh) * 2008-01-08 2008-07-16 东南大学 一种毛细管微流控芯片
CN103616427B (zh) * 2013-12-02 2016-05-04 中国科学院上海应用物理研究所 一种针对前列腺癌不同类型血清标志物进行同时检测的微流控电化学生物传感系统
CN104931711A (zh) * 2015-06-25 2015-09-23 马鞍山海普微奇生物科技有限公司 一种促进生物试剂与生物分子结合的方法和装置
CN107966558A (zh) * 2017-11-20 2018-04-27 中国科学院苏州生物医学工程技术研究所 一种用于体外诊断的目标靶分子快速捕获方法
CN109557150A (zh) * 2019-01-14 2019-04-02 大连大学 数字微流控芯片及基于其的病原体免疫检测方法
CN109499636B (zh) * 2019-01-14 2024-02-27 大连大学 一种病原体免疫检测数字微流控芯片及其制作方法

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111781379A (zh) * 2020-07-16 2020-10-16 天津中新科炬生物制药股份有限公司 一种用于新型冠状病毒联合检测的微流控芯片模组
CN112964865A (zh) * 2021-01-18 2021-06-15 中山大学 一种通道形貌控制流体混合的超快速免疫检测芯片及其制备方法和应用
WO2023284686A1 (fr) * 2021-07-12 2023-01-19 西湖大学 Procédé et système de détection par biodétection pour des anticorps de liaison
CN116679049A (zh) * 2023-02-27 2023-09-01 科赫生物科技(北京)有限公司 一种免疫检测方法

Also Published As

Publication number Publication date
WO2021169089A1 (fr) 2021-09-02

Similar Documents

Publication Publication Date Title
CN111337662A (zh) 一种基于微流控芯片的快速免疫检测方法
Hosseini et al. Advantages, disadvantages and modifications of conventional ELISA
CN101620227B (zh) 基于结构型导电高分子材料技术的霍乱诊断用多通道芯片
TWI545323B (zh) 一種離心式磁性粒子操縱與檢測裝置及其運作方法
CN109870582B (zh) 一种多靶标磁免疫化学发光微流控芯片检测平台及方法
US8530230B2 (en) Multiplexed assay methods
CN101587123A (zh) 带有一维自组装磁珠链电极的诊断霍乱专用微流控芯片
CN110252434B (zh) 一种用于微流控芯片的液体储存结构及微流控芯片
KR20200142174A (ko) 측면 흐름 분석-기반의 면역 검출용 접합체 및 이를 사용한 면역 검출 방법
JP2007101221A (ja) 超高速で生体分子反応を測定する方法
CN111033237B (zh) 使用剂量响应曲线的递减信号部分来测量包括高浓度分析物在内的分析物的夹心式测定
US20070166703A1 (en) Method of detecting pathogenic microorganism in real-time, using modified flow-type surface plasmon resonance biosensor
Lu et al. Dissolution-enhanced luminescence enhanced digital microfluidics immunoassay for sensitive and automated detection of H5N1
CN113433329A (zh) 基于量子点荧光微球的pct/il-6二联检测试剂盒及其制备方法
CN110496658B (zh) 一种联合诊断纸基微流控芯片及制备方法
CN210752736U (zh) 一种用于微流控芯片的液体储存结构及微流控芯片
Kakuta et al. Development of the microchip-based repeatable immunoassay system for clinical diagnosis
CN113311160A (zh) 快速检测SARS-CoV-2抗原和IgG/IgM抗体的微流控生物芯片
WO2021184494A1 (fr) Procédé de fabrication de capteur, capteur et procédé de détection de capteur
EP1351056B1 (fr) Procédé d'analyse de liaison spécifique et dispositif correspondant
JP7369017B2 (ja) コロイド粒子を用いた被検物質検出増感法
CN111936852B (zh) 区分细菌感染和病毒感染的多重横向流测定物
US20230358747A1 (en) In vitro method for the detection of sars-cov-2 in a sample from the upper respiratory tract using a colorimetric immunosensor and related colorimetric immunosensor
CN111801575B (zh) 区分细菌感染和病毒感染的多重横向流测定物
Li et al. Fully Integrated and High‐Throughput Microfluidic System for Multiplexed Point‐Of‐Care Testing

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination