CN1113223A - Method and equipment for production of multi-effect organic biological fertilizer - Google Patents
Method and equipment for production of multi-effect organic biological fertilizer Download PDFInfo
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- CN1113223A CN1113223A CN 95114866 CN95114866A CN1113223A CN 1113223 A CN1113223 A CN 1113223A CN 95114866 CN95114866 CN 95114866 CN 95114866 A CN95114866 A CN 95114866A CN 1113223 A CN1113223 A CN 1113223A
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Abstract
A multiple effect organic biological fertilizer manufacturing method and the equipment utilizes microorganism mixed culture and a special made box-type multi-layer solid fermentation device to process one raw material, that is, the livestock manure. The processes are: the livestock manure is fermented, deodorized, and slaked; the livestock manure is mixed with inorganic nitrogen, phosphorus and kalium fertilizer and minor elements of zinc and boric fertilizer; the billet granulation is made into fertilizer semi-manufactured products; a plurality of microorganisms are made into live bacteria mixed culture bacterial fertilizer semi-manufactured products after the fermentation; and then the two semi-manufactured products are packed into finished products. The method and equipment for producing multiple effect organic biological fertilizer does not discharge malodorous gas into the environment. The fermentation equipment takes up less space and operates conveniently. The manufacture efficiency is high and the cost is low.
Description
The present invention is the production method and the equipment of relevant a kind of novel multi-effect organic biological fertilizer.Be specifically related to the microorganism mixed culture A of the raw materials pretreatment of this fertilizer, raw materials pretreatment fermentation unit, and the beneficial microorganism mixed culture B in this novel multi-effect organic biological fertilizer of composition.
Organic bio-fertilizer can directly influence plant physiology, promote the crop stalwartness, improve disease resistance, promote the soil microorganisms activity, activating soil, suppress salt fertilizer overrich to the infringement of crop with to the pollution of environment, the required conventional nitrogen of plant growth, phosphorus, potassium nutrition and requisite trace element are provided comprehensively, thereby reach long-acting efficient.Therefore, many in recent years national organic bio-fertilizers quietly rise.Domestic having developed at present to regulate the microniological proudcts of plant physiology, as " 9,20 ", " 5406 ", " planting precious plain ", " yield increasing fungus ", to supply with fertilizer, " pure fertilizer ", " bio-fertilizer " and " compoiste fertilizer ", " the fertility height " formed with cicken feces dried and inorganic nitrogen, phosphorus, potassium, Japanese " the biological light " produced etc. that crop alimentary is taken as the leading factor.
Domestic manure of livestock and poultry processing becomes fertilizer that three kinds of approach are arranged: the one, and the hot blast convection drying, the manure of livestock and poultry of moisture 60-70% is directly used the roasting kiln warm air drying,, oven dry difficulty big because of bright excrement viscosity, thermo-efficiency is low, and institute's air draft stench, serious environment pollution, the 2nd, far infrared is handled, and the freshly-slaughtered poultry excrement processing with moisture 60% becomes particle, because of the cost height, promotes difficulty; The 3rd, the slot type aerobic fermentation utilizes luminous energy and bioenergy, by regular oxygenation, constantly evaporation, fermentation, can drop to water content about 20% to the chicken manure of water content 60% by 40 days aerobic fermentations, and its processing costs height, time are long.
The purpose of this invention is to provide a kind of manure of livestock and poultry that utilizes microorganism mixed culture and special device to handle one of raw material, and utilize the bacterium mixed culture to produce the method for multi-effect organic biological fertilizer, this production method is not to the environmental emission foul gas, it is convenient that fermentation equipment is taken up an area of less, used, the production efficiency height, cost is low.
The production method of this multiple-effect organic bio-fertilizer is:
(1) the microbial strains bacterial strain that will have a recognition feature is CCTCC NO M95018 at the preserving number of China's preservation on the 1st in typical culture collection center April nineteen ninety-five, NO M95017 CCTCC NO AA93002 and NO AA92017, through fermentation culture, make mixed culture A, and and manure of livestock and poultry, the straw powder, the peat composed of rotten mosses mixes, in the box solid fermentation device that the present invention proposes, spontaneous fermentation was handled 5-7 days under normal temperature (15-65 ℃) normal pressure, deodorization, slaking, and with inorganic material NPK fertilizers and trace element zinc, boron fertilizer is mixed thoroughly, through squeezing the base granulation, make the fertilizer work in-process.
(2) the multiple microbial strains bacterial strain that will have a recognition feature is CCTCC NO M95020 at the preserving number of China's preservation on the 1st in typical culture collection center April nineteen ninety-five, NO M95019, NO M95021, CCTCC NO AB95018 and NO AB95001, cultivation and fermentation is mixed and made into mixed culture B bacterial manure work in-process with the viable bacteria of fermenting.
(3) above-mentioned (1), (2) two kinds of work in-process are separately packed, (1): (2) according to required weight than, as 1: 0.05-0.1 attaches together in a finished product packing bag, whenever is bundled into product fertilizer and contains fertilizer work in-process 45-47.5 kilogram, bacterial manure work in-process 2.5-5.0 kilogram.
Before use in the field, after unpacking,, can make base manure or topdress two kinds of work in-process mixings.
The bacterial classification that mixed culture A relates to has:
(1) Nocardia bacteria (Nocardia sp.) bacterial strain, corresponding preserving number CCTCC NO M95018 on yeast extract paste one malt extract nutrient agar, forms substrate mycelium, with the passing of time be fractured into rhabdos, on agar plate, bacterium colony protuberance, baby pink, do not produce aerial mycelium, on fructose, glucose, produce acid, can utilize aesculin, reduction nitrate does not solidify not freezing milk yet, and is good 28 ℃ of following incubation growth.
(2), separate the refined Rockwell yeast of fat (Yallowia llpolytica) bacterial strain, corresponding preserving number CCTCC NO M95017 is in glucose-yeast extract paste-peptone liquid nutrient medium, cultivated 3 days for 25 ℃, cell is avette to microscler, produces bacterium, on the wort agar inclined-plane, cultivate, the bacterium colony oyster white, sticking wet, matt, form pseudohypha, utilize glucose, do not utilize semi-lactosi, sucrose, raffinose, assimilation ammonium sulfate does not utilize nitrate.
(3), the green and brown streptomycete of peacock (Streptomyces malachitofuscus) bacterial strain, corresponding preserving number CCTCC NO AA93002, on No. 1 agar of Gao Shi, 28 ℃ of cultivations, substrate mycelium is orange, the aerial mycelium beige, the fibrillae of spores volution, spore surface is barbed, produce melanochrome, utilize the glucose several kinds of carbon source, do not utilize raffinose.
(4) give birth to black streptomycete (Streptomyces nigrifacienf) bacterial strain, corresponding preserving number CCTCC NO AA92017, on No. 1 nutrient agar of Gao Shi, 28 ℃ of cultivations, substrate mycelium light orange, aerial mycelium beige, fibrillae of spores ripple song, spore surface is smooth, does not produce melanochrome, utilizes several kinds of carbon source such as glucose, fructose, wood sugar, raffinose.
Public offering on A flora (3), (4) bacterial strain market.
The A colony culture medium:
(1) separates the refined Rockwell yeast of fat slant medium, use potato agar, according to a conventional method preparation.
(2) separate the refined Rockwell yeast of fat fermention medium, wheat bran 35%, Semen Maydis powder 10%, sucrose 3%, ammonium sulfate 1.5%, lime carbonate 0.5%, straw powder 50%(weight), be modulated into PH6-7 with 2% liming.
(3) the Nocardia bacteria bacteria culture medium is given birth to the actinomycetic actinomycetes substratum of cultivating week with the B flora of black streptomycete with the green and brown streptomycete of bacteria culture medium peacock of B flora.
The bacterial classification that mixed culture B relates to has:
Aerobacillus polymyxa Donker (Bacillus polymyxa) bacterial strain, corresponding preserving number CCTCC NO M95020, lawn is thin on nutrient agar medium, the expansion growth, cell is shaft-like, and size is 0.7-0.8 * 2-5 μ m, and peritrichous is arranged, and forms gemma, the gemma ellipse is positioned at an end, and sporangiocyst obviously expands.Main positive physiological and biochemical property has: catalase; Anaerobic growth; The VP test; Produce sour aerogenesis from glucose, pectinose, wood sugar and N.F,USP MANNITOL, produce Protosol; Hydrolyzed starch, hydrolyzed casein and gelatin; Nitrate reduction becomes nitrite; Grow at PH5.7; Fixed member nitrogen.Main negative physiological and biochemical property has: utilize Citrate trianion, decompose cruel propylhomoserin, the phenylalanine deamination produces lecithinase, produces indoles; 7%NaCl growth etc.
(2) wax sample bacillus (Bacillus cereus) bacterial strain, corresponding preserving number CCTCC NO M95019, bacterium colony is bordering on circle on nutrient agar medium, and lawn is ceraceous, and cell is shaft-like, and size is 1.0-1.1 * 2.5-5.0 μ m, and peritrichous is arranged.Form gemma, the gemma ellipse is positioned at sporangiocyst central authorities, and sporangiocyst does not expand.Main positive physiology feature has: catalase; Anaerobic growth; The VP test; Produce acid from glucose; Hydrolyzed starch; Hydrolyzed casein and gelatin; Decompose tyrosine; Utilize Citrate trianion; Nitrate reduction becomes nitrite; Produce lecithinase; In PH5.7 and 7%NaCl growth.Main negative physiological and biochemical property has: produce acid from pectinose, wood sugar and N.F,USP MANNITOL; Produce indoles; Phenylalanine deamination and 10%NaCl growth.
(3) genus bacillus (Baclllus sp.) bacterial strain, corresponding preserving number CCTCC NO AB95018 all can grow on nutrient agar medium and nitrogen-free agar, colony colourless, the shaft-like 0.8-0.9 of cell * 2.5-4.5 μ m, form gemma, the gemma ellipse is positioned at central authorities.Anaerobism is not grown, the catalase positive, and hydrolyzed starch and gelatin do not produce acid from glucose, pectinose, wood sugar and N.F,USP MANNITOL.
(4) streptomycete (Streptomgces sp.) bacterial strain, corresponding preserving number is CCTCC NO AA95001, on synthetic agar, the aerial hyphae grey, the brown little purple of base silk produces the brown water colo(u)r.The fibrillae of spores volution.
(5) false single crisp bacterium (Pseudomonas sp.) bacterial strain, corresponding preserving number is CCTCC NO M95021, bacterium colony circle on nutrient agar medium, green entirely, produce fluorochrome, cell is shaft-like, 0.4 * 1.0-1.8 μ m, Gram-negative, have and extremely give birth to feathering, strict aerobic, do not grow at 41 ℃ and 4 ℃, catalase, oxydase and the lecithinase positive.
(3) of B flora and (4) are public offering in the city.
The B colony culture medium:
1, strain inclined plane substratum
(1) bacillus polymyxa, bacillus cereus, genus bacillus, pseudomonas bacterium are used extractum carnis-peptone-yeast extract medium, and preparing burden is peptone 1.0%, extractum carnis 0.3%, and yeast extract paste 0.5%, agar 1.5%, all the other are water, PH7.2-7.1.
(2) the streptomycete actinomycetes are synthesized No. 1 agar with Gao Shi, and preparing burden is saltpetre 0.1%, dipotassium hydrogen phosphate 0.05%, and sal epsom 0.05%, sodium-chlor 0.05%, ferrous sulfate 0.001%, Zulkovsky starch 2%, agar 2%, all the other are water, PH7.2-7.4.
2, solid seed enlarged culturing base
Weight batching: wheat bran 30%, soybean cake powder 25%, Semen Maydis powder 24%, peptone 0.1%, white sugar 0.4%, sal epsom 0.03%, potassium primary phosphate 0.02%, lime carbonate 0.45%, rice bran 20% is mixed thoroughly with 2-3% liming, and amount of water is about 0.8-1.0 times of siccative, about PH10.
3, fermention medium
Weight batching, wheat bran 30%, soybean cake powder 25%, Semen Maydis powder 24.5%, calcium superphosphate 0.5%, system chaff 20% is mixed thoroughly with 2-3% liming, and amount of water is 0.8-1.0 a times of siccative.
Each bacterial strain that mixed culture A and mixed culture B relate to, cultivate into the bacterial classification seed with corresponding substratum routine separately, conventional expansion becomes the solid seed, and solid fermentation becomes to contain the bacterium product, then single culture product balanced mix is got microorganism mixed culture A, mixed culture B.
Mixed culture A and manure of livestock and poultry, straw powder, the peat composed of rotten mosses are mixed the used box multilayer of thing (this equipment example is six layers) solid fermentation apparatus structure thoroughly as scheming:
Fig. 1 is box six layers of solid fermentation device configuration diagram
Fig. 2 is box six layers of solid fermentation device side-view
Fig. 3 is box six layers of solid fermentation general ability portion enlarged view
Box fermentor shown in the figure is that metal structure has six layers, by a framework 1, and a plurality of strip turnover panels 2,3, six traces 4 of the connecting rod identical with the turnover panel number and six pneumatic means 5 form. Every layer has a plurality of turnover panels 2 side by side, each turnover panel 2 is movably arranged on the framework 1 by the shelf 6 of its underpart and the axle 7 at two ends, one of each axle 7 with vertical the fixing of connecting rod 3 one ends, the other end of the connecting rod 3 that every one deck is all and same trace 4 usefulness bearing pins 8 are flexibly connected, each trace 4 is being driven by a pneumatic means 5 and is moving back and forth, thereby drivening rod 3 swings and turnover panel 2 rotate around axle 7, make Turnover panel 2 is set level or is tilted, and during inclination the fermentation materials 9 on the turnover panel is poured on lower one deck fermentation bed. For making air-flow interlayer in fermentor can come smoothly diffraction current, the turnover panel termination of adjacent bed interlocks and stays opening. Arrow represents the air-flow trend among the figure. The top has removable cover, and sleety weather and winter add a cover when temperature is low.
During operation with the mixture material of mixed culture A and manure of livestock and poultry, straw powder, the peat composed of rotten mosses with machinery or manually add this fermentor from top, in normal pressure, bed temperature 15-65 ℃ lower spontaneous fermentation, approximately every day, every layer of turnover panel 2 tumbled once, material general fermentation in winter in fermentor 7 days, 5 days summers, be about 6 days season in spring and autumn, and the material that ferments is discharged from the bottom. Then with inorganic N,P,K fertilizers and trace element zinc, boron fertilizer mix, through the particle that comminutor is made 1-6mm pulverize, cross screen out coarse, cooling packings after 80 ℃ of lower oven dry. Temperature was crossed when hanging down and was passed into warm wind from the fermentor bottom winter, and the four seasons produce continuously.
1000 kilograms of manure of livestock and poultry; 150 kilograms in the straw powder of pulverizing; 150 kilograms of the peats composed of rotten mosses; mix thoroughly with the mixing microorganisms culture A50 kilogram that makes with preceding method; the travelling belt that is equipped with the box solid fermentation device with the present invention's design promotes; and change fermentation container over to; handled 7 days 15-65 ℃ of following spontaneous fermentation; after the discharging; with 300 kilograms in urea; 450 kilograms of general calcium; 150 kilograms of mixs of Repone K; pulverize; cross and screen out thick slag, squeeze embryo through tablets press and cause the 1-6 mm granules, 80 ℃ of oven dry; cooling; generate organic deodorization slaking fertilizer finished product, be distributed into 45 kilograms every bag fertilizer work in-process.
Streptomycete (ray fungi bacterial strain) CCTCC NO AA95001, routine is cultivated into the bacterial classification seed, and conventional expansion becomes the solid seed, and solid fermentation becomes to contain the bacterium product.
The bacterium class bacterial strain spore bacillus CCTCC NO M95020 that sticks to one's teeth more, bacillus cereus CCTCC NO M95019, genus bacillus CCTCC NO AB95018, pseudomonas CCTCC NO M95021, routine is cultivated into the bacterial classification seed, and routine is expanded into the solid seed, and solid fermentation becomes to contain the bacterium product.
Mix into above-mentioned ray fungi thoroughly mixed bacterium, 40 ℃ of dryings of low temperature with the bacterium product balanced mix that contains of bacterium class fermentation, generate the bacterial manure work in-process.These bacterial manure work in-process are divided into every bag of 2.5 kilograms of packings,, get multiple-effect organic-biological fertilizer product with above-mentioned 45 kilograms of fertilizer work in-process sinteticses packing.
Embodiment 2
1000 kilograms of manure of livestock and poultry, 150 kilograms in the straw powder of pulverizing, 150 kilograms of the peats composed of rotten mosses are mixed thoroughly with the mixing microorganisms culture A50 kilogram that makes with preceding method, and 25-65 ℃ of following spontaneous fermentation was handled 5 days in aforementioned fermentation container, after the discharging with 550 kilograms of ammonium chlorides, 135 kilograms of phosphorus ammoniums, 180 kilograms of vitriolate of tartar, 1.5 kilograms of zinc fertilizers, 1.5 kilograms of boron fertilizers, squeeze embryo granulation, 80 ℃ and dry, cool off, are distributed into every bag of 47.5 kilograms of fertilizer work in-process mix, pulverizing.
Make the bacterial manure work in-process by embodiment 1, pack 5 kilograms for every bag, fit in the packing of product, get multiple-effect organic-biological fertilizer product with above-mentioned 47.5 kilograms of fertilizer work in-process.
The multi-effect organic biological fertilizer that embodiment 1,2 produces through the check of technical survey department, meets Hubei Province Q/DLSH
1-93Standard.Useful total viable count 〉=2.0 hundred million/gram, thermotolerance bacterium 〉=1,800,000/gram, organism 〉=45%, humic acids 〉=4%, full nitrogen 〉=6.5%, full phosphorus 〉=4.0%, full potassium 〉=4.5%, free-water＜12.0%, 1-6 mm granules＞90%, PH6.4.
Change manure of livestock and poultry and straw powder, the ratio of the peat composed of rotten mosses, with the ratio of mineral manure, and the bacterial manure ratio, can make the fertilizer that is suitable for Different Crop growth use.
Make fertilizer in enormous quantities by example 1,2 methods, be used for the farm crop test, before the use, fertilizer work in-process and bacterial manure work in-process are fully mixed thoroughly.
On cotton crop, use with other effect of fertilizer and be compared as follows:
This multiple-effect organic bio-fertilizer is executed 90 kilograms of 80 kilograms of average yield per mu gined cottons for every mu
The fertile mu of the compound of ternary compound fertilizer 25% is with 50 kilograms 82.1 kilograms
Fertilizer, cotton seed cake are executed 80 kilograms 85.5 kilograms for every mu
2500 kilograms 75.5 kilograms in pig cattle pen excrement of contrast (habits fertilization) mu
Claims (4)
1, a kind of multi-effect organic biological fertilizer production method is characterized in that:
(1) the multiple microorganism that will have recognition feature is made mixed culture A, and mix with manure of livestock and poultry, straw powder, the peat composed of rotten mosses, spontaneous fermentation was handled 5-7 days under normal temperature in box solid fermentation device (15-65 ℃) normal pressure, deodorization, slaking, and mix thoroughly with inorganic material NPK fertilizers and trace element zinc, boron fertilizer, squeeze the base granulation, make the fertilizer work in-process
(2) will have recognition feature multiple microorganism strains cultivation and fermentation, make mixed culture B bacterial manure work in-process,
(3) above-mentioned (1), (2) two kinds of work in-process are separately packed, (1): (2) are according to required weight than being loaded in the finished product packing bag.
2, method according to claim 1, it is characterized in that said microorganism mixed culture A is meant by Nocardia bacteria (Nocardia sp) bacterial strain CCTCCNOM95018, separate the refined Rockwell yeast of fat (Yallowia lipolytica) bacterial strain CCTCC NO SP M95017, give birth to black streptomycete (Streptomyces nigrifacienfs) bacterial strain CCTCC NO AA92021, the Chinese typical culture collection center preservation before April 1 nineteen ninety-five of the mixed bacterial that the green and brown streptomycete of peacock (Streptomyces malachitofuscus) bacterial strain CCTCC NO AA93002 forms, above-mentioned bacterial strains in Wuhan.
3, method according to claim 1, it is characterized in that said microorganism mixed culture B is meant the M95020 by the spore bacillus that sticks to one's teeth (Bacillus polymyra) bacterial strain CCTCC NO more, bacillus cereus (Bacillus cerens) bacterial strain CCTCC NO M95019, genus bacillus (Bacillus SP.) bacterial strain CCTCC NO AB95018, the Chinese typical culture collection center preservation before April 1 nineteen ninety-five of the mixed bacterial that streptomycete (Sfreptomyces SP.) bacterial strain CCTCC NO AA95001 and pseudomonas (Pseadomanas SP.) bacterial strain CCTCC NO M95021 form, above-mentioned bacterial strains in Wuhan.
4, the box solid fermentation device that is used for the described production method of claim 1, it is characterized in that by a framework (1), a plurality of long strip shape turnover panels (2), the connecting rod (3) identical with the turnover panel number, trace (4) and pneumatics (5) are formed, each turnover panel (2) is movably arranged on upward formation multilayer fermentation bed of framework (1) by the axle (7) at shelf (6) in the middle of its underpart and shelf two ends, every layer has a plurality of turnover panels (2) side by side, one and connecting rod (3) one end vertical fixing of each axle (7), the other end of every layer of all connecting rod (3) and same trace (4) flexibly connect with bearing pin (8), each trace (4) is being driven by a pneumatics (5) and is moving back and forth, be with connecting rod (3) swing, and turnover panel (2) rotates around axle (7), makes turnover panel (2) set level or tilt.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95114866 CN1113223A (en) | 1995-04-14 | 1995-04-14 | Method and equipment for production of multi-effect organic biological fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 95114866 CN1113223A (en) | 1995-04-14 | 1995-04-14 | Method and equipment for production of multi-effect organic biological fertilizer |
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| CN1113223A true CN1113223A (en) | 1995-12-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 95114866 Pending CN1113223A (en) | 1995-04-14 | 1995-04-14 | Method and equipment for production of multi-effect organic biological fertilizer |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010249A (en) * | 2010-10-29 | 2011-04-13 | 西安新天地草业有限公司 | Method for preparing composite biological organic fertilizer |
| CN102267824A (en) * | 2010-06-04 | 2011-12-07 | 南开大学 | Efficient microorganism solid state fermentation reaction apparatus and use method thereof |
| CN102617007A (en) * | 2012-04-13 | 2012-08-01 | 厦门华净环保技术有限公司 | Three-dimensional multi-layer type animal manure treatment device |
| CN101993263B (en) * | 2004-03-22 | 2012-08-22 | 康普-安尼有限公司 | Method for building and operatin aerobic waste treatment system |
| CN101781145B (en) * | 2010-01-29 | 2013-07-31 | 南京工业大学 | Preparation method of liquid fertilizer containing high phosphorus and potassium |
-
1995
- 1995-04-14 CN CN 95114866 patent/CN1113223A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101993263B (en) * | 2004-03-22 | 2012-08-22 | 康普-安尼有限公司 | Method for building and operatin aerobic waste treatment system |
| CN101781145B (en) * | 2010-01-29 | 2013-07-31 | 南京工业大学 | Preparation method of liquid fertilizer containing high phosphorus and potassium |
| CN102267824A (en) * | 2010-06-04 | 2011-12-07 | 南开大学 | Efficient microorganism solid state fermentation reaction apparatus and use method thereof |
| CN102010249A (en) * | 2010-10-29 | 2011-04-13 | 西安新天地草业有限公司 | Method for preparing composite biological organic fertilizer |
| CN102010249B (en) * | 2010-10-29 | 2012-10-31 | 西安新天地草业有限公司 | Method for preparing composite biological organic fertilizer |
| CN102617007A (en) * | 2012-04-13 | 2012-08-01 | 厦门华净环保技术有限公司 | Three-dimensional multi-layer type animal manure treatment device |
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