CN111321189A

CN111321189A - Cordyceps militaris polypeptide and preparation method thereof

Info

Publication number: CN111321189A
Application number: CN202010196831.6A
Authority: CN
Inventors: 赵一鸣
Original assignee: Chengdu Liuran Medical Technology Co ltd
Current assignee: Chengdu Liuran Medical Technology Co ltd
Priority date: 2020-03-19
Filing date: 2020-03-19
Publication date: 2020-06-23
Anticipated expiration: 2040-03-19
Also published as: CN111321189B

Abstract

The invention belongs to the technical field of polypeptide preparation, and particularly relates to cordyceps militaris polypeptide and a preparation method thereof, wherein the preparation method comprises the steps of crushing cordyceps militaris by a crusher, putting the crushed cordyceps militaris into a stirring barrel, adding distilled water, timing for 90-120 minutes from boiling, and naturally cooling to room temperature to obtain cordyceps militaris solution; adding alkaline protease to realize enzymolysis of Cordyceps militaris solution, controlling pH of Cordyceps militaris solution at 10.0-11.0, controlling enzymolysis temperature at 50-55 deg.C, adding alkaline protease 5.12% of Cordyceps militaris solution, and controlling enzymolysis time at 11-12 hr to obtain enzymolysis solution; and then carrying out decoloration treatment to obtain a decolored solution, and sequentially filtering, concentrating and spray-drying the decolored solution by a concentrating device to finally obtain the cordyceps militaris polypeptide.

Description

Cordyceps militaris polypeptide and preparation method thereof

Technical Field

The invention belongs to the technical field of polypeptide preparation, and particularly relates to cordyceps militaris polypeptide and a preparation method thereof.

Background

Cordyceps militaris is a medicinal and edible fungus which is in the same genus as Cordyceps, has the same main medicinal components as Cordyceps militaris, and has the effects of regulating immunity, inhibiting tumor, delaying aging, etc. Cordyceps militaris polypeptide separated from Cordyceps militaris stroma has antibacterial, anticancer and immunity enhancing effects. The main active ingredients of the cordyceps militaris mainly comprise cordyceps polysaccharide, adenosine, mannitol, SOD enzyme and some trace elements. More reports are currently made about cordycepin and cordyceps polysaccharide, and the research on preparing polypeptides from proteins and the development of health care products are less.

The application number of 2017104697812 provides a cordyceps militaris polypeptide and a preparation method thereof, wherein the preparation method comprises the following steps: s1, drying and crushing the cordyceps militaris, adding the cordyceps militaris into distilled water, boiling for 1-2h, and cooling to room temperature to obtain cordyceps militaris water solution; s2, adjusting the pH value of the cordyceps militaris aqueous solution to 5-9, and then adding complex enzyme to carry out enzymolysis on protein and cellulose in the cordyceps militaris aqueous solution to obtain an enzymolysis solution; s3, filtering and decolorizing the enzymolysis liquid to obtain decolorized liquid, and sequentially performing membrane filtration, concentration and spray drying on the decolorized liquid to obtain cordyceps militaris polypeptide; the preparation method can not effectively extract the polypeptide in the cordyceps militaris, and can further improve the extraction rate of the polypeptide substance in the cordyceps militaris.

In view of this, in order to overcome the above technical problems, the present company has designed and developed a cordyceps militaris polypeptide and a preparation method thereof, and a special preparation method is adopted to solve the above technical problems.

Disclosure of Invention

In order to make up for the defects of the prior art and solve the problems described in the background art, the invention provides cordyceps militaris polypeptide and a preparation method thereof.

The technical scheme adopted by the invention for solving the technical problems is as follows: the preparation method of the cordyceps militaris polypeptide comprises the following steps:

s1: firstly, exposing the cordyceps militaris to the sun, controlling the moisture of the cordyceps militaris to be 7-8 dry, then cleaning the soil adhered to the cordyceps militaris, stir-frying the cordyceps militaris in a frying pan, controlling the moisture of the cordyceps militaris to be more than 9 dry, then crushing the cordyceps militaris by a crusher, and filtering the cordyceps militaris by a 80-mesh screen;

s2: putting the powder cordyceps militaris filtered in the S1 into a stirring barrel, adding distilled water, timing for 90-120 minutes from boiling, and naturally cooling to room temperature to obtain cordyceps militaris solution;

s3: adding alkaline protease into the cordyceps militaris solution in the S2 to realize enzymolysis of the cordyceps militaris solution, controlling the pH of the cordyceps militaris solution to be 10.0-11.0, controlling the enzymolysis temperature to be 50-55 ℃, controlling the adding amount of the alkaline protease to be 5.12% of the cordyceps militaris solution, and controlling the enzymolysis time to be 11-12 hours, thereby finally obtaining an enzymolysis solution;

s4: and (3) filtering and decolorizing the enzymolysis liquid in the S3 by using 20-mesh active carbon, then treating for 10-15 minutes by using a centrifuge with 10000rpm to obtain decolorized liquid, and sequentially filtering, concentrating and spray drying the decolorized liquid by using a concentrating device to finally obtain the cordyceps militaris polypeptide.

Preferably, before the powdered cordyceps militaris in the S2 is dissolved in distilled water, degreasing treatment is carried out by using degreasing liquid, and the volume percentage concentration of the degreasing liquid is 90-99% of ethanol water solution; when the cordyceps militaris is degreased, the pH value of enzymolysis of a cordyceps militaris solution is controlled to be 10.0-11.0 and is alkaline, so that an aqueous solution with relatively weak acidity is used for degreasing, the degreasing solution generally comprises three types of ethanol aqueous solution, ethyl acetate and petroleum ether, the acidity of the ethanol aqueous solution is less than that of the ethyl acetate, the petroleum ether has toxicity and is not suitable for degreasing, so that the ethanol aqueous solution is selected as the degreasing solution of the cordyceps militaris, and when the decoloring solution is dried by a concentrating device, the ethanol in the decoloring solution can be evaporated, and the preparation of cordyceps militaris polypeptide cannot be influenced.

Preferably, when the enzymolysis liquid in the S4 is subjected to filtering and decoloring treatment by activated carbon, the filtering and decoloring treatment temperature is kept at 75-80 ℃, the enzymolysis liquid is dissolved in an acetic acid solution, the volume percentage concentration of the acetic acid is 20-30%, and the decoloring time is controlled within 45-65 minutes; the activated carbon has strong adsorption capacity in an aqueous solution with pH of 3-6, an acetic acid aqueous solution is used for filtering and decoloring, and when the decolored solution is dried by a concentration device, the acetic acid in the decolored solution can be evaporated, so that the preparation of the cordyceps militaris polypeptide is not influenced.

Preferably, the concentrator device comprises a housing and an end cap; the end cover is connected to the upper end of the shell through a bolt, a U-shaped frame is arranged at the upper end of the end cover, a motor is arranged in the center of the upper end of the U-shaped frame, and an output shaft of the motor is connected with a filtering unit arranged in the shell; the filtering unit comprises grinding balls, a sieve plate and a drainage plate; the grinding ball is hollow hemispherical, a plurality of first-size through holes are formed in the spherical surface of the grinding ball, the upper end surface of the grinding ball is connected with an output shaft of a motor, feed ports are symmetrically formed in the upper end surface of the grinding ball, the outer ring of the grinding ball is hinged to a U-shaped frame through a support, and a sieve plate is arranged below the grinding ball; the sieve plate is arranged in a shape corresponding to the grinding balls, the upper end of the sieve plate is fixedly connected to the end cover, a plurality of second through holes are formed in the spherical surface of the sieve plate, the diameter of each second through hole is smaller than that of each first through hole, and a drainage plate is arranged below the sieve plate; the outer ring of the drainage plate is fixedly connected to the inner side wall of the shell, a third through hole is formed in the center of the drainage plate, and a drying unit is arranged below the third through hole; firstly, pouring a decoloring liquid into a grinding ball from a feeding port, then starting a motor to rotate, driving the grinding ball to rotate by the motor, then leading the decoloring liquid to be acted by centrifugal force in the grinding ball, throwing the decoloring liquid out of a first through hole and entering a space between the grinding ball and a sieve plate, simultaneously grinding the unbroken cordyceps militaris particles in the decoloring liquid on the surface of the grinding ball to grind the cordyceps militaris between the grinding ball and the sieve plate again, then filtering the decoloring liquid through the sieve plate and flowing on a drainage plate, and then leading the decoloring liquid to flow into a drying unit through a third through hole;

the drying unit comprises a first pipeline, an evaporation plate and an evaporation barrel; the first pipelines are symmetrically erected in the shell, one end of each first pipeline is connected with external dry steam, and the other end of each first pipeline is vertically arranged and communicated with the hollow part in the evaporation plate; the middle of the evaporation plate is high, the outer ring of the evaporation plate is low, and the center of the upper end of the evaporation plate is communicated with the evaporation barrel; a liquid outlet is reserved at the joint of the lower end of the evaporation barrel and the evaporation plate, an evaporation tube for evaporating destaining solution is arranged in the evaporation barrel, and the evaporation tube is electrically connected with an external power supply; the lower end of the shell is funnel-shaped, and a discharge pipe is arranged in the center of the lower end of the shell; through the cooperation among the motor, the filtering unit and the drying unit, the filtering, the concentration and the drying of the decolored liquid are realized; before in the decoloration liquid flows into the evaporating bucket, the evaporating pipe in the evaporating bucket is led into the current and is generated heat, after decoloration liquid flows into the evaporating bucket, moisture boiling in the decoloration liquid is evaporated, then decoloration liquid trickles on the evaporating plate through the liquid outlet, because it has dry hot-air to let in the evaporating plate, make the evaporating plate surface heat the evaporation once more to the decoloration, make the moisture in the decoloration liquid evaporated once more, cordyceps militaris polypeptide has been prepared this moment, then cordyceps militaris polypeptide falls into the casing bottom and takes out from the discharging pipe from the evaporating plate.

Preferably, the evaporation tube is communicated with the hollow part in the evaporation plate, and a plurality of air outlet holes are formed in the outer ring of the evaporation tube; the drying and evaporation of the decolored liquid by the evaporation barrel are enhanced through the air outlet; after the decolored liquid flows into the evaporation barrel, the evaporation tube is introduced with current to generate heat and evaporate the decolored liquid, and simultaneously, the air outlet hole on the evaporation tube sprays dry hot gas body liquid to evaporate and dry the decolored liquid; after gas is sprayed out from the gas outlet, the gas enables the decolored liquid to boil in the evaporation barrel and generate bubbles, so that the decolored liquid can be fully contacted with the gas, and the moisture in the decolored liquid can be effectively evaporated.

Preferably, the evaporating plate is provided with a plurality of circles of bulges; the bulges are arranged on the evaporation plate in a terrace shape; the flowing speed of the destaining solution on the evaporation plate is slowed down through the bulges, and the evaporation time of the destaining solution is prolonged; after the destaining solution flows on the evaporation plate from the liquid outlet, the destaining solution is hindered by the bulge, the flowing speed of the destaining solution on the evaporation plate is reduced, the retention time of the destaining solution on the evaporation plate is prolonged, the evaporation plate can be contacted with the destaining solution for a longer time, and therefore the evaporation effect of water in the destaining solution is improved.

Preferably, the inner part of each circle of the bulges is hollow, and each circle of the bulges is provided with a plurality of gas injection pipes; the air injection direction of the air injection pipe is arranged along the inclined surface of the evaporation plate; the drying and air-spraying evaporation of the decolored liquid by the evaporation plate is further improved through the air-spraying pipe; decoloration liquid trickles when the evaporating plate, decoloration liquid receives the bellied hindrance of many rings, simultaneously gas in the evaporating plate is through protruding intraductal blowout of air jet from the jet-propelled, and make decoloration liquid further obtain the evaporation at the evaporating plate behind the jet-propelled pipe blowout gas, simultaneously gas blows and raises decoloration liquid, make the contact thickness attenuation of decoloration liquid also evaporating plate, be of value to the evaporation of decoloration liquid, and the jet-propelled direction of jet-propelled roller, make partial decoloration liquid obtain the hindrance once more, thereby prolong decoloration liquid dwell time on the evaporating plate once more, then moisture in the decoloration liquid obtains effective evaporation.

Preferably, the side wall of the grinding ball is provided with annular teeth, the annular teeth are positioned between the bracket and the end cover, and the annular teeth are meshed with a first gear arranged on the end cover; the first gear is connected with the fan blades through a first rod, and the first rod is hinged in an exhaust hole formed in the end cover; a plurality of fourth through holes are formed in the drainage plate close to the inner side wall of the shell; through the matching of the annular teeth, the first gear rod, the fan blades and the fourth through hole, the water vapor in the shell is discharged; when the grinding balls rotate, the number gear is driven to rotate through the annular teeth, and then the number-one gear copper drives the fan blades to rotate through the number-one rod; after the fan blades rotate, the fan blades extract the evaporated moisture in the shell through the fourth passage; the water evaporated from the decolorized solution is separated in time, and the phenomenon that the purification rate of cordyceps militaris polypeptide is reduced due to the fact that the evaporated water is condensed into water beads and drops on the bottom of the shell is avoided.

A Cordyceps militaris polypeptide is prepared by the above preparation method.

The invention has the technical effects and advantages that:

1. according to the cordyceps militaris polypeptide and the preparation method thereof, alkaline protease is added into cordyceps militaris solution to realize enzymolysis of the cordyceps militaris solution, the activity of the alkaline protease for hydrolyzing protein peptide bonds is strongest when the pH value of the alkaline protease is 9.0-11.0, and the pH value of the cordyceps militaris solution is controlled to be 10.0-11.0, so that the activity of the alkaline protease can be ensured, the inactivation of the alkaline protease can be avoided, the enzymolysis of the cordyceps militaris solution is improved, and the extraction rate of the cordyceps militaris polypeptide is improved.

2. According to the cordyceps militaris polypeptide and the preparation method thereof, cordyceps militaris polypeptide particles in a decoloration liquid are ground again between the grinding balls and the sieve plate through the matching between the grinding balls and the sieve plate; through the cooperation between evaporation barrel, evaporating pipe and the evaporating plate for in the decoloration liquid evaporating barrel through electric heating and jet-propelled heating flow on the evaporating plate again wait to heat evaporation moisture, thereby the moisture in the decoloration liquid obtains effectual evaporation, improves the extraction rate of cordyceps militaris polypeptide in turn.

Drawings

The invention is further described with reference to the following figures and embodiments.

FIG. 1 is a flow chart of a preparation method of the present invention;

FIG. 2 is a perspective view of the concentration device of the present invention;

FIG. 3 is a cross-sectional view of a thickening apparatus according to the present invention;

FIG. 4 is an enlarged view of a portion of FIG. 3 at A;

FIG. 5 is a partial enlarged view at B in FIG. 3;

in the figure: the device comprises a shell 1, a discharge pipe 11, an end cover 2, a motor 21, a first gear 22, a first rod 221, fan blades 222, an exhaust hole 23, a filtering unit 3, a grinding ball 31, a first through hole 311, a feeding port 312, an annular tooth 313, a sieve plate 32, a second through hole 321, a flow guide plate 33, a third through hole 331, a fourth through hole 332, a first pipeline 41 of a drying unit 44, an evaporation plate 42, a protrusion 421, an air injection pipe 422, an evaporation barrel 43, a liquid outlet 431, an evaporation pipe 432 and an air outlet 433.

Detailed Description

In order to make the technical means, the creation features, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the following embodiments.

Example 1

Adding pepsin into 10KG cordyceps militaris solution, wherein the amount of the pepsin is 5.12% of the amount of the cordyceps militaris solution, the enzymolysis time is controlled to be 11 hours, the pH of the cordyceps militaris solution is controlled to be 7.0-9.0, and the enzymolysis temperature is controlled to be 50-55 ℃, decoloring the obtained enzymolysis solution, filtering, concentrating and spray drying the decolored solution in sequence by a concentrating device to obtain cordyceps militaris polypeptide, calculating the extraction rate of the cordyceps militaris polypeptide, and recording the extraction rate in a table I;

example 2

Adding papain into 10KG cordyceps militaris solution, wherein the amount of the papain is 5.12 percent of the amount of the cordyceps militaris solution, the enzymolysis time is controlled to be 11 hours, the pH of the cordyceps militaris solution is controlled to be 7.0-9.0, and the enzymolysis temperature is controlled to be 50-55 ℃, so that obtained enzymolysis solution is decolorized, and then the decolorized solution is sequentially filtered, concentrated and spray-dried by a concentration device, so that cordyceps militaris polypeptide is finally obtained, the extraction rate of the cordyceps militaris polypeptide is calculated, and the extraction rate is recorded in a table I;

example 3

Adding helicase into 10KG cordyceps militaris solution, wherein the amount of the helicase is 5.12% of the amount of the cordyceps militaris solution, the enzymolysis time is controlled to be 11 hours, the pH of the cordyceps militaris solution is controlled to be 7.0-9.0, and the enzymolysis temperature is controlled to be 50-55 ℃, so that obtained enzymolysis liquid is decolorized, and then the decolorized liquid is sequentially filtered, concentrated and spray-dried by a concentration device, so that cordyceps militaris polypeptide is finally obtained, the extraction rate of the cordyceps militaris polypeptide is calculated, and the extraction rate is recorded in a table I;

example 4

Adding alkaline protease into 10KG cordyceps militaris solution, wherein the amount of the alkaline protease is 5.12% of the amount of the cordyceps militaris solution, the enzymolysis time is controlled to be 11 hours, the pH of the cordyceps militaris solution is controlled to be 7.0-9.0, and the enzymolysis temperature is controlled to be 50-55 ℃, decoloring the obtained enzymolysis solution, filtering, concentrating and spray drying the decolored solution in sequence by a concentrating device to obtain cordyceps militaris polypeptide, calculating the extraction rate of the cordyceps militaris polypeptide, and recording the extraction rate in a table I;

table one

Is in the total percentage

Time of enzymolysis

PH

Temperature of enzymolysis

Extraction ratio (%)

Example 1

Pepsin

5.12％

11 hours

7.0-9.0

50-55℃

57.23

Example 2

Papain

5.12％

11 hours

7.0-9.0

50-55℃

48.46

Example 3

Snail enzyme

5.12％

11 hours

7.0-9.0

50-55℃

67.25

Example 4

Alkaline protease

5.12％

11 hours

7.0-9.0

50-55℃

85.23

From the examples 1 to 4, it is concluded that the extraction rate of the cordyceps militaris polypeptide in the example 4 is the highest by using the controlled variable method under the condition of ensuring that the total percentage of the added enzyme, the enzymolysis time, the pH and the enzymolysis temperature are the same.

As shown in fig. 1 to 5, the concentrator of the present invention includes a housing 1 and an end cap 2; the end cover 2 is connected to the upper end of the shell 1 through a bolt, a U-shaped frame is arranged at the upper end of the end cover 2, a motor 21 is arranged at the center of the upper end of the U-shaped frame, and an output shaft of the motor 21 is connected with the filtering unit 3 arranged in the shell 1; the filtering unit 3 comprises grinding balls 31, a sieve plate 32 and a drainage plate 33; the grinding ball 31 is a hollow hemispherical shape, the spherical surface of the grinding ball 31 is provided with a plurality of one-size through holes 311, the upper end surface of the grinding ball 31 is connected with an output shaft of the motor 21, the upper end surface of the grinding ball 31 is symmetrically provided with feeding ports 312, the outer ring of the grinding ball 31 is hinged on a U-shaped frame through a support, and a sieve plate 32 is arranged below the grinding ball 31; the sieve plate 32 is arranged in a shape corresponding to the grinding balls 31, the upper end of the sieve plate 32 is fixedly connected to the end cover 2, a plurality of second through holes 321 are formed in the spherical surface of the sieve plate 32, the diameter of each second through hole 321 is smaller than that of each first through hole 311, and a drainage plate 33 is arranged below the sieve plate 32; the outer ring of the drainage plate 33 is fixedly connected to the inner side wall of the shell 1, a third through hole 331 is formed in the center of the drainage plate 33, and a drying unit 44 is arranged below the third through hole 331; firstly, a decoloring liquid is poured into the grinding ball 31 from the feeding port 312, then the motor 21 is started to rotate, the motor 21 drives the grinding ball 31 to rotate, then the decoloring liquid is under the action of centrifugal force in the grinding ball 31, the decoloring liquid is thrown out from the first through hole 311 and enters between the grinding ball 31 and the sieve plate 32, meanwhile, the surface of the grinding ball 31 is used for grinding cordyceps militaris particles which are not crushed in the decoloring liquid, so that the cordyceps militaris is ground between the grinding ball 31 and the sieve plate 32 again, then the decoloring liquid is filtered by the sieve plate 32 and flows on the drainage plate 33, and then the decoloring liquid flows into the drying unit 44 through the third through hole 331;

the drying unit 44 includes a first pipe 41, an evaporation plate 42 and an evaporation barrel 43; the first pipelines 41 are symmetrically erected in the shell 1, one end of each first pipeline 41 is connected with outside dry steam, and the other end of each first pipeline 41 is vertically arranged and communicated with the hollow part in the evaporation plate 42; the middle of the evaporation plate 42 is high, the outer ring is low, and the center of the upper end of the evaporation plate 42 is communicated with the evaporation barrel 43; a liquid outlet 431 is reserved at the joint of the lower end of the evaporation barrel 43 and the evaporation plate 42, an evaporation tube 432 for evaporating destaining solution is arranged in the evaporation barrel 43, and the evaporation tube 432 is electrically connected with an external power supply; the lower end of the shell 1 is funnel-shaped, and a discharge pipe 11 is arranged at the center of the lower end of the shell 1; through the cooperation among the motor 21, the filtering unit 3 and the drying unit 4, the filtering, the concentrating and the drying of the decolored liquid are realized; before the decolored liquid flows into the evaporation barrel 43, the evaporation tube 432 in the evaporation barrel 43 is charged with current to generate heat, after the decolored liquid flows into the evaporation barrel 43, the water in the decolored liquid is boiled and evaporated, then the decolored liquid flows on the evaporation plate 42 through the liquid outlet 431, because dry hot air is introduced into the evaporation plate 42, the surface of the evaporation plate 42 is heated and evaporated for decoloring again, the water in the decolored liquid is evaporated again, the cordyceps militaris polypeptide is prepared at the moment, and then the cordyceps militaris polypeptide falls into the bottom of the shell 1 from the evaporation plate 42 and is taken out from the discharge pipe 11.

As a specific embodiment of the present invention, the evaporation tube 432 is communicated with the hollow inside of the evaporation plate 42, and a plurality of air outlet holes 433 are formed on the outer ring of the evaporation tube 432; the drying and evaporation of the decolored liquid by the evaporation barrel 43 are enhanced through the air outlet 433; after the destaining solution flows into the evaporation barrel 43, the evaporation tube 432 is electrified to generate heat to evaporate the destaining solution, and simultaneously, the air outlet 433 on the evaporation tube 432 sprays dry hot gas liquid to evaporate and dry the destaining solution; after gas is sprayed out of the gas outlet 433, the gas enables the destaining solution to boil in the evaporation barrel 43 and generate bubbles, so that the destaining solution can be fully contacted with the gas, and the moisture in the destaining solution can be effectively evaporated.

As a specific embodiment of the present invention, the evaporating plate 42 is provided with a plurality of circles of protrusions 421; the protrusions 421 are arranged on the evaporation plate 42 in a terrace shape; the flowing speed of the destaining solution on the evaporation plate 42 is slowed down through the bulges 421, and the evaporation time of the destaining solution is prolonged; after the destaining solution flows on the evaporation plate 42 from the liquid outlet 431, the destaining solution is hindered by the protrusion 421, the flowing speed of the destaining solution on the evaporation plate 42 is reduced, the retention time of the destaining solution on the evaporation plate 42 is prolonged, the evaporation plate 42 can be contacted with the destaining solution for a longer time, and thus the evaporation effect of water in the destaining solution is improved.

As a specific embodiment of the present invention, the inside of each circle of the protrusions 421 is hollow, and a plurality of gas nozzles 422 are arranged on each circle of the protrusions 421; the air injection direction of the air injection pipe 422 is arranged along the inclined surface of the evaporation plate 42; the drying and air-spraying evaporation of the decolored liquid by the evaporation plate 42 is further improved through the air-spraying pipe 422; decoloration liquid trickles when evaporating plate 42, decoloration liquid receives the hindrance of many rings of protruding 421, simultaneously gas in the evaporating plate 42 is through protruding 421 from spouting in the jet-propelled pipe 422, and jet-propelled pipe 422 spouts makes decoloration liquid further obtain the evaporation at evaporating plate 42 after gaseous, simultaneously gas blows and rises decoloration liquid, make decoloration liquid also evaporating plate 42's contact thickness attenuation, be of value to the evaporation of decoloration liquid, and the jet-propelled direction of jet-propelled roller, make partial decoloration liquid obtain hindrance once more, thereby lengthen decoloration liquid dwell time on evaporating plate 42 once more, then moisture in the decoloration liquid obtains effective evaporation.

As a specific embodiment of the present invention, the side wall of the grinding ball 31 is provided with an annular tooth 313, and the annular tooth 313 is located between the bracket and the end cap 2, and the annular tooth 313 is engaged with the first gear 22 provided on the end cap 2; the first gear 22 is connected with the fan blade 222 through a first rod 221, and the first rod 221 is hinged in an exhaust hole 23 formed in the end cover 2; a plurality of fourth through holes 332 are formed in the position, close to the inner side wall of the shell 1, of the drainage plate 33; through the matching among the annular teeth 313, the first gear 22, the first rod 221 and the fan blades 222 and the fourth through holes 332, the water vapor in the shell 1 is discharged; when the grinding ball 31 rotates, the ring-shaped teeth 313 drive the first gear to rotate, and then the first gear 22 copper drives the fan blades 222 to rotate through the first rod 221; after the fan blades 222 rotate, the fan blades 222 extract the evaporated moisture in the housing 1 through the fourth passage; the water evaporated from the decolorized solution is separated in time, and the phenomenon that the purification rate of cordyceps militaris polypeptide is reduced due to the fact that the evaporated water is condensed into water beads and drops on the bottom of the shell 1 is avoided.

A Cordyceps militaris polypeptide is prepared by the above preparation method.

When the grinding ball mill works, firstly, a decoloration liquid is poured into the grinding ball 31 from the feeding port 312, then the motor 21 is started to rotate, the motor 21 drives the grinding ball 31 to rotate, then the decoloration liquid is under the action of centrifugal force in the grinding ball 31, the decoloration liquid is thrown out from the first through hole 311 and enters between the grinding ball 31 and the sieve plate 32, meanwhile, the surface of the grinding ball 31 is used for grinding the cordyceps militaris particles which are not ground in the decoloration liquid, so that the cordyceps militaris is ground between the grinding ball 31 and the sieve plate 32 again, then the decoloration liquid is filtered by the sieve plate 32 and flows on the drainage plate 33, and then the decoloration liquid flows into the drying unit 44 through the third through hole 331; before the decolored liquid flows into the evaporation barrel 43, current is introduced into an evaporation tube 432 in the evaporation barrel 43 to generate heat, when the decolored liquid flows into the evaporation barrel 43, moisture in the decolored liquid is boiled and evaporated, then the decolored liquid flows on the evaporation plate 42 through a liquid outlet 431, dry hot air is introduced into the evaporation plate 42, the surface of the evaporation plate 42 is heated and evaporated again for decoloration, so that the moisture in the decolored liquid is evaporated again, cordyceps militaris polypeptide is prepared at the moment, and then the cordyceps militaris polypeptide falls into the bottom of the shell 1 from the evaporation plate 42 and is taken out from the discharge pipe 11; after the destaining solution flows into the evaporation barrel 43, the evaporation tube 432 is electrified to generate heat to evaporate the destaining solution, and simultaneously, the air outlet 433 on the evaporation tube 432 sprays dry hot gas liquid to evaporate and dry the destaining solution; after the gas is sprayed out of the gas outlet 433, the gas enables the destaining solution to boil in the evaporation barrel 43 and generate bubbles, so that the destaining solution can be fully contacted with the gas, and the moisture in the destaining solution can be effectively evaporated; after the destaining solution flows on the evaporation plate 42 from the liquid outlet 431, the destaining solution is hindered by the protrusion 421, the flowing speed of the destaining solution on the evaporation plate 42 is reduced, the retention time of the destaining solution on the evaporation plate 42 is prolonged, so that the evaporation plate 42 can be contacted with the destaining solution for a longer time, and the evaporation effect of water in the destaining solution is improved; when the decolored liquid flows on the evaporation plate 42, the decolored liquid is hindered by the plurality of circles of bulges 421, meanwhile, gas in the evaporation plate 42 is sprayed out from the gas spraying pipe 422 through the bulges 421, the decolored liquid is further evaporated on the evaporation plate 42 after the gas spraying pipe 422 sprays the gas, and simultaneously the gas is blown to raise the decolored liquid, so that the contact thickness of the decolored liquid and the evaporation plate 42 is reduced, the evaporation of the decolored liquid is facilitated, the gas spraying direction of the gas spraying roller is also used for enabling part of the decolored liquid to be hindered again, the retention time of the decolored liquid on the evaporation plate 42 is prolonged again, and then, the moisture in the decolored liquid is effectively evaporated; when the grinding ball 31 rotates, the ring-shaped teeth 313 drive the first gear to rotate, and then the first gear 22 copper drives the fan blades 222 to rotate through the first rod 221; after the fan blades 222 rotate, the fan blades 222 extract the evaporated moisture in the housing 1 through the fourth passage; the water evaporated from the decolorized solution is separated in time, and the phenomenon that the purification rate of cordyceps militaris polypeptide is reduced due to the fact that the evaporated water is condensed into water beads and drops on the bottom of the shell 1 is avoided.

The foregoing illustrates and describes the principles, essential features and advantages of the invention in operation. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are given by way of illustration of the principles of the present invention, and that various changes and modifications may be made without departing from the spirit and scope of the invention as defined by the appended claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A preparation method of cordyceps militaris polypeptide is characterized by comprising the following steps: the preparation method comprises the following steps:

s3: adding alkaline protease into the cordyceps militaris solution in the S2 to realize enzymolysis of the cordyceps militaris solution, controlling the pH of the cordyceps militaris solution to be 6.0-10.0, controlling the enzymolysis temperature to be 50-55 ℃, controlling the adding amount of the alkaline protease to be 5.12% of the cordyceps militaris solution, and controlling the enzymolysis time to be 11-12 hours, thereby finally obtaining an enzymolysis solution;

2. The method for preparing cordyceps militaris polypeptide according to claim 1, wherein the method comprises the following steps: and (3) degreasing the powdery cordyceps militaris in the S2 by using degreasing liquid before dissolving the powdery cordyceps militaris in distilled water, wherein the degreasing liquid is an ethanol water solution with the volume percentage concentration of 90-99%.

3. The method for preparing cordyceps militaris polypeptide according to claim 1, wherein the method comprises the following steps: when the enzymolysis liquid in the S4 is filtered and decolored by activated carbon, the temperature of the filtering and decoloring treatment is kept at 75-80 ℃, the enzymolysis liquid is dissolved in an acetic acid solution, the volume percentage concentration of the acetic acid is 20-30%, and the decoloring time is controlled within 45-65 minutes.

4. The method for preparing cordyceps militaris polypeptide according to claim 1, wherein the method comprises the following steps: the concentrating device comprises a shell (1) and an end cover (2); the end cover (2) is connected to the upper end of the shell (1) through a bolt, a U-shaped frame is arranged at the upper end of the end cover (2), a motor (21) is arranged at the center of the upper end of the U-shaped frame, and an output shaft of the motor (21) is connected with a filtering unit (3) arranged in the shell (1); the filtering unit (3) comprises grinding balls (31), a sieve plate (32) and a drainage plate (33); the grinding ball (31) is hollow and hemispherical, a plurality of one-size through holes (311) are formed in the spherical surface of the grinding ball (31), the upper end face of the grinding ball (31) is connected with an output shaft of the motor (21), feed ports (312) are symmetrically formed in the upper end face of the grinding ball (31), the outer ring of the grinding ball (31) is hinged to the U-shaped frame through a support, and a sieve plate (32) is arranged below the grinding ball (31); the sieve plate (32) is arranged in a shape corresponding to the grinding balls (31), the upper end of the sieve plate (32) is fixedly connected to the end cover (2), a plurality of second through holes (321) are formed in the spherical surface of the sieve plate (32), the diameter of each second through hole (321) is smaller than that of each first through hole (311), and a drainage plate (33) is arranged below the sieve plate (32); the outer ring of the drainage plate (33) is fixedly connected to the inner side wall of the shell (1), a third through hole (331) is formed in the center of the drainage plate (33), and a drying unit (4) is arranged below the third through hole (331);

the drying unit (4) comprises a first pipeline (41), an evaporation plate (42) and an evaporation barrel (43); the first pipelines (41) are symmetrically erected in the shell (1), one end of each first pipeline (41) is connected with outside dry steam, and the other end of each first pipeline (41) is vertically arranged and communicated with a hollow part in the evaporation plate (42); the middle of the evaporation plate (42) is high, the outer ring is low, and the center of the upper end of the evaporation plate (42) is communicated with an evaporation barrel (43); a liquid outlet (431) is reserved at the joint of the lower end of the evaporation barrel (43) and the evaporation plate (42), an evaporation tube (432) for evaporating destaining solution is arranged in the evaporation barrel (43), and the evaporation tube (432) is electrically connected with an external power supply; the lower end of the shell (1) is funnel-shaped, and a discharge pipe (11) is arranged in the center of the lower end of the shell (1); through the cooperation among motor (21), filtering unit and drying unit (4), realize filtering, concentrating and drying to the decoloration liquid.

5. The method for preparing cordyceps militaris polypeptide according to claim 4, wherein the method comprises the following steps: the evaporation tube (432) is communicated with the hollow part in the evaporation plate (42), and a plurality of air outlet holes (433) are formed in the outer ring of the evaporation tube (432); the drying and evaporation of the decolored liquid by the evaporation barrel (43) are enhanced through the air outlet hole (433).

6. The method for preparing cordyceps militaris polypeptide according to claim 4, wherein the method comprises the following steps: a plurality of circles of bulges (421) are arranged on the evaporation plate (42); the protrusions (421) are distributed on the evaporation plate (42) in a terrace shape; the flow speed of the destaining solution on the evaporation plate (42) is slowed down through the bulges (421), and the evaporation time of the destaining solution is prolonged.

7. The method for preparing cordyceps militaris polypeptide according to claim 6, wherein the method comprises the following steps: the inner part of each circle of the bulges (421) is hollow, and a plurality of gas injection pipes (422) are arranged on each circle of the bulges (421); the air injection direction of the air injection pipe (422) is arranged along the inclined surface of the evaporation plate (42); the drying air-jet evaporation of the decoloration liquid by the evaporation plate (42) is further improved through the air-jet pipe (422).

8. The method for preparing cordyceps militaris polypeptide according to claim 4, wherein the method comprises the following steps: the side wall of the grinding ball (31) is provided with annular teeth (313), the annular teeth (313) are positioned between the support and the end cover (2), and the annular teeth (313) are meshed with a first gear (22) arranged on the end cover (2); the first gear (22) is connected with the fan blades (222) through a first rod (221), and the first rod (221) is hinged in an exhaust hole (23) formed in the end cover (2); a plurality of fourth through holes (332) are formed in the position, close to the inner side wall of the shell (1), of the drainage plate (33); through the cooperation of the annular teeth (313), the first gear (22), the first rod (221) and the fan blades (222) and the fourth through hole (332), the water vapor in the shell (1) is discharged.

9. A cordyceps militaris polypeptide is characterized in that: is prepared by the preparation method of any one of claims 1 to 8.