CN111315784A - 新的抗原结合嵌合蛋白及其方法和用途 - Google Patents

新的抗原结合嵌合蛋白及其方法和用途 Download PDF

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Publication number
CN111315784A
CN111315784A CN201880071396.5A CN201880071396A CN111315784A CN 111315784 A CN111315784 A CN 111315784A CN 201880071396 A CN201880071396 A CN 201880071396A CN 111315784 A CN111315784 A CN 111315784A
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protein
antigen
binding
domain
scaffold
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Chinese (zh)
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J·斯泰亚特
E·帕顿
T·乌坎斯基
W·弗兰肯
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Vlaams Instituut voor Biotechnologie VIB
Universite Libre de Bruxelles ULB
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Vlaams Instituut voor Biotechnologie VIB
Universite Libre de Bruxelles ULB
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
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    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C07K16/464—Igs containing CDR-residues from one specie grafted between FR-residues from another
    • C07K16/465—Igs containing CDR-residues from one specie grafted between FR-residues from another with additional modified FR-residues
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/20—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/22—Immunoglobulins specific features characterized by taxonomic origin from camelids, e.g. camel, llama or dromedary
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/00023—Virus like particles [VLP]

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  • Biotechnology (AREA)
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  • Wood Science & Technology (AREA)
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  • Urology & Nephrology (AREA)
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  • Crystallography & Structural Chemistry (AREA)
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  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201880071396.5A 2017-10-31 2018-10-31 新的抗原结合嵌合蛋白及其方法和用途 Pending CN111315784A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP17199472.6 2017-10-31
EP17199472 2017-10-31
EP18199709 2018-10-10
EP18199709.9 2018-10-10
PCT/EP2018/079891 WO2019086548A1 (en) 2017-10-31 2018-10-31 Novel antigen-binding chimeric proteins and methods and uses thereof

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CN111315784A true CN111315784A (zh) 2020-06-19

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US (2) US11873347B2 (https=)
EP (2) EP3704160B1 (https=)
JP (2) JP7760115B2 (https=)
KR (2) KR20200091400A (https=)
CN (1) CN111315784A (https=)
CA (1) CA3076791A1 (https=)
WO (1) WO2019086548A1 (https=)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110982824A (zh) * 2019-10-09 2020-04-10 天津大学 拮抗pd-1的抗体类似物bp基因及蛋白和应用
CN111518180A (zh) * 2020-04-10 2020-08-11 四川大学 一种结核病候选疫苗融合蛋白
CN114540328A (zh) * 2022-02-18 2022-05-27 浙江工业大学 一种温度敏感型α-淀粉酶及其制备方法与应用
CN115819599A (zh) * 2022-11-29 2023-03-21 江苏耀海生物制药有限公司 一种利用重组大肠杆菌分泌表达纳米抗体Cablivi的方法

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JP7627910B2 (ja) * 2018-12-21 2025-02-07 フエー・イー・ベー・フエー・ゼツト・ウエー サイトカイン及び足場タンパク質を含む融合タンパク質
EP3986437A4 (en) * 2019-06-18 2023-06-07 Musc Foundation for Research Development COMPOSITIONS TARGETED AGAINST THE NUCLEOTIDE-FREE STATE OF RAS AND METHODS FOR BLOCKING ONCOGENIC SIGNALING AND TRANSFORMATION
US20220378925A1 (en) * 2019-09-26 2022-12-01 Orionis Biosciences, Inc. Conjugated chimeric proteins
EP3799881A1 (en) 2019-10-04 2021-04-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Single domain antibodies specifically binding globo - series glycans
WO2021078786A1 (en) 2019-10-21 2021-04-29 Vib Vzw Nanodisc-specific antigen-binding chimeric proteins
US20240027467A1 (en) * 2019-12-20 2024-01-25 Vib Vzw Nanobody Exchange Chromatography
EP3845553A1 (en) * 2019-12-30 2021-07-07 Johann Wolfgang Goethe-Universität Carrier matrix comprising dodecin protein
JP7657406B2 (ja) * 2020-02-17 2025-04-07 国立大学法人大阪大学 膜タンパク質の結晶化方法及び膜タンパク質の構造解析方法
US20230382977A1 (en) * 2020-09-24 2023-11-30 The Regents Of The University Of California Multimeric protein complexes as antibody substitutes for neutralization of viral pathogens in prophylactic and therapeutic applications
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US12050189B2 (en) * 2021-09-10 2024-07-30 President And Fellows Of Harvard College Compositions and methods relating to structural determination of small proteins
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US20250084130A1 (en) * 2023-08-25 2025-03-13 Trustees Of Boston University Engineered mcp and pcp proteins and systems and methods thereof
WO2025061726A1 (en) * 2023-09-18 2025-03-27 John Innes Centre Vlp-scaffold based binding proteins
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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110982824A (zh) * 2019-10-09 2020-04-10 天津大学 拮抗pd-1的抗体类似物bp基因及蛋白和应用
CN110982824B (zh) * 2019-10-09 2022-04-15 天津大学 拮抗pd-1的抗体类似物bp基因及蛋白和应用
CN111518180A (zh) * 2020-04-10 2020-08-11 四川大学 一种结核病候选疫苗融合蛋白
CN111518180B (zh) * 2020-04-10 2023-01-03 四川大学 一种结核病候选疫苗融合蛋白
CN114540328A (zh) * 2022-02-18 2022-05-27 浙江工业大学 一种温度敏感型α-淀粉酶及其制备方法与应用
CN115819599A (zh) * 2022-11-29 2023-03-21 江苏耀海生物制药有限公司 一种利用重组大肠杆菌分泌表达纳米抗体Cablivi的方法
CN115819599B (zh) * 2022-11-29 2024-01-16 江苏耀海生物制药有限公司 一种利用重组大肠杆菌分泌表达纳米抗体Cablivi的方法

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JP2023138991A (ja) 2023-10-03
EP3704160B1 (en) 2025-08-20
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