CN111308680A - Linear lighting device and gene sequencer - Google Patents
Linear lighting device and gene sequencer Download PDFInfo
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- CN111308680A CN111308680A CN201811515785.0A CN201811515785A CN111308680A CN 111308680 A CN111308680 A CN 111308680A CN 201811515785 A CN201811515785 A CN 201811515785A CN 111308680 A CN111308680 A CN 111308680A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0032—Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- G—PHYSICS
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- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- G—PHYSICS
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- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/09—Beam shaping, e.g. changing the cross-sectional area, not otherwise provided for
- G02B27/0938—Using specific optical elements
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- G02B27/0966—Cylindrical lenses
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Abstract
A linear illumination device and a gene sequencer, the linear illumination device comprising an illumination module and a microscope objective for receiving light transmitted through the illumination module, the illumination module comprising: the optical fiber is used for projecting outlet light spots; the first cylindrical lens receives the light emitted by the optical fiber; the second cylindrical lens receives light rays emitted by the first cylindrical lens and projects the outlet light spots on an image surface through the microscope objective to form illumination light spots, wherein the outlet light spots are zoomed by the first cylindrical lens and the microscope objective in a first direction, and the outlet light spots are zoomed by the second cylindrical lens and the microscope objective in a second direction to form linear illumination light spots. Like this, through the focus that sets up first cylindrical lens, second cylindrical lens and micro objective, can adjust the size and the shape of illumination facula, light path simple structure, the debugging is convenient.
Description
Technical Field
The invention relates to the field of gene sequencing equipment, in particular to a linear lighting device and a gene sequencer.
Background
The statements in this section merely provide background information related to the present disclosure and may not necessarily constitute prior art.
The gene sequencer illuminates the object plane with laser and images to the CCD camera through a microscope. In the prior art, the illumination light spot can be generated by a cylindrical lens for illumination. However, it is difficult to adjust parameters such as the width, length, and shape of the linear light.
Disclosure of Invention
In view of the above, there is a need for a linear illumination device and a gene sequencer that facilitate adjustment of the illumination spot.
A linear illumination device comprising an illumination module and a microscope objective for receiving light transmitted through the illumination module, the illumination module comprising:
the optical fiber is used for projecting outlet light spots;
the first cylindrical lens receives the light emitted by the optical fiber;
the second cylindrical lens receives light rays emitted by the first cylindrical lens and projects the outlet light spots on an image surface through the microscope objective to form illumination light spots, wherein the outlet light spots are zoomed by the first cylindrical lens and the microscope objective in a first direction, and the outlet light spots are zoomed by the second cylindrical lens and the microscope objective in a second direction to form linear illumination light spots.
Preferably, the cross-section of the core of the optical fiber is rectangular, so that the exit spot is rectangular.
Preferably, a length direction of the first cylindrical lens is perpendicular to a length direction of the second cylindrical lens, the first cylindrical lens and the microscope objective lens enlarge the length direction of the exit light spot, and the second cylindrical lens and the microscope objective lens reduce the width direction of the exit light spot to form a linear illumination light spot on the image plane.
Preferably, the first cylindrical lens is one of a single lens, a cemented lens and a lens group; the second cylindrical lens is one of a single lens, a cemented lens and a lens group.
Preferably, the method further comprises the following steps:
the base comprises a through hole which passes through two ends;
a first lens mechanism for connecting the first cylindrical lens to one end of the through hole of the base;
the second lens mechanism is used for connecting the second cylindrical lens to the other end of the through hole of the base;
and the optical fiber mechanism is used for connecting the optical fiber to one side of the first cylindrical lens, which is far away from the second cylindrical lens, so that the light rays emitted from the optical fiber sequentially pass through the first cylindrical lens and the second cylindrical lens.
Preferably, the first lens mechanism includes:
the first fixing piece is connected to the end part of the base along the axial direction of the base;
the first lens bonding piece is sleeved on the peripheral surface of the first cylindrical lens and is movably connected to the first fixing piece along the axial direction of the first fixing piece, and when the first lens bonding piece moves relative to the first fixing piece, the first lens bonding piece is close to or far away from the second cylindrical lens;
and the first locking jackscrew penetrates through the first fixing piece along the radial direction of the first lens bonding piece and abuts against the peripheral surface of the first lens bonding piece so as to lock the first lens bonding piece.
Preferably, the optical fiber mechanism includes:
the optical fiber connector comprises a through hole and is connected to the first fixing piece along the axial direction;
the optical fiber fixing piece is connected with the optical fiber in the middle, movably connected with the optical fiber connecting piece along the axial direction of the optical fiber connecting piece, and at least arranged in the through hole of the optical fiber connecting piece at the end part, and drives the optical fiber to be close to or far away from the first cylindrical lens when the optical fiber fixing piece moves relative to the optical fiber connecting piece;
and the second locking jackscrew penetrates through the peripheral surface of the optical fiber connector along the radial direction of the optical fiber fixing piece and abuts against the peripheral surface of the optical fiber fixing piece so as to lock the optical fiber fixing piece.
Preferably, the second lens mechanism includes:
the second fixing piece is connected to the end part of the base along the axial direction of the base through threads, and when the second fixing piece rotates relative to the base, the second fixing piece is close to or far away from the first cylindrical lens;
the second lens bonding piece is sleeved on the circumferential surface of the second cylindrical lens and is in threaded connection with the second fixing piece, and the second lens bonding piece is opposite to the second fixing piece and drives the second cylindrical lens to be close to or far away from the first cylindrical lens when the second fixing piece rotates.
Preferably, the second lens mechanism further includes:
the connecting seat is arranged on the second fixing piece, and the second lens bonding piece is connected to the connecting seat through threads;
and the adjusting screw penetrates through the second fixing piece along the radial direction of the connecting seat and abuts against the peripheral surface of the connecting seat so as to adjust the position of the second cylindrical lens along the radial direction.
A gene sequencer comprises the linear illumination device, and illumination light spots are imaged on an image plane by light rays emitted by the linear illumination device.
Compared with the prior art, the linear illumination device and the gene sequencer have the advantages that the first cylindrical lens and the microobjective zoom the outlet light spots emitted by the optical fibers in the first direction, the second cylindrical lens and the microobjective zoom the outlet light spots in the second direction to form linear illumination light spots, so that the size and the shape of the illumination light spots can be adjusted by setting the focal lengths of the first cylindrical lens, the second cylindrical lens and the microobjective or the angle between the first cylindrical lens and the second cylindrical lens, the light path structure is simple, and the debugging is convenient.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on these drawings without creative efforts.
Fig. 1 is a schematic structural view of a linear lighting device.
Fig. 2 shows the illumination spots imaged on the image plane by the linear illuminator.
Fig. 3 is a schematic structural diagram of the lighting module.
Fig. 4 is a schematic sectional view at IV-IV in fig. 3.
Fig. 5 is a schematic structural view of the base and the first lens mechanism.
Fig. 6 is a schematic sectional structure view of the base and the first lens mechanism.
Fig. 7 is a schematic structural view of the first fixing member and the optical fiber mechanism.
Fig. 8 is a schematic cross-sectional view of the first fixing member and the optical fiber mechanism.
Fig. 9 is a schematic structural view of the base and the second lens mechanism.
Fig. 10 is a schematic sectional structure view of the base and the second lens mechanism.
Fig. 11 is a partial cross-sectional view of the lighting module illustrating its internal structure, with only some of the component parts being cross-hatched for ease of reading the figure.
Description of the main elements
First cylindrical lens 11
Second cylindrical lens 12
Connecting socket 152
Second lens adhesive member 153
Adjusting screw 154
First fixing member 161
First lens adhesive member 162
First locking jackscrew 163
Optical fiber fixing member 171
The following detailed description will further illustrate the invention in conjunction with the above-described figures.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, a detailed description of the present invention will be given below with reference to the accompanying drawings and detailed description, as shown in fig. 1. In addition, the embodiments and features of the embodiments of the present application may be combined with each other without conflict. In the following description, numerous specific details are set forth to provide a thorough understanding of the present invention, and the described embodiments are merely a subset of the embodiments of the present invention, rather than a complete embodiment. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention.
In various embodiments of the present invention, for convenience in description and not in limitation, the term "coupled" as used in the specification and claims of the present application is not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. "Up", "down", "left", "right", etc. are used only to indicate a relative positional relationship, which changes when the absolute position of the object being described changes, accordingly.
Fig. 1 is a schematic structural view of a linear lighting device. As shown in fig. 1, the line illumination device includes an illumination module 10 and a microscope objective 20. The illumination module 10 is used for emitting light, the microscope objective 20 is used for receiving the light penetrating through the illumination module 10, and the illumination module 10 and the microscope objective 20 cooperate to form an illumination light spot to be imaged on an object plane of a microscope.
As shown in fig. 1, the illumination module 10 includes an optical fiber 13, a first cylindrical lens 11, and a second cylindrical lens 12.
The optical fiber 13 is used to introduce laser light, which is input from an input end of the optical fiber 13 and output an exit spot from an output end. In this embodiment, at least the cross section of the core at the output end of the optical fiber 13 is rectangular, i.e. rectangular or square, so that the exit spot output by the optical fiber 13 is rectangular.
The first cylindrical lens 11 is used for receiving the light emitted by the optical fiber 13 and zooming the exit light spot in a first direction with the microscope objective lens 20. The first cylindrical lens 11 is one of a single lens, a cemented lens, and a lens group.
The second cylindrical lens 12 receives the light emitted through the first cylindrical lens 11 and projects the exit light spot on an image plane through the microscope objective lens 20 to form an illumination light spot, and the second cylindrical lens 12 and the microscope objective lens 20 zoom the exit light spot in a second direction. The second cylindrical lens 12 is one of a single lens, a cemented lens and a lens group, and is preferably a double cemented lens, and can correct chromatic aberration between two wavelengths. After being emitted from the output end of the optical fiber 13, the laser sequentially passes through the first cylindrical lens 11, the second cylindrical lens 12 and the microscope objective lens 20, and then forms an illumination spot on an image plane. Since the first cylindrical lens 11 and the microscope objective lens 20 zoom the first direction of the exit spot, changing the focal length of the first cylindrical lens 11 and the microscope objective lens 20 can change the zoom magnification of the first direction. Also, the second cylindrical lens 12 and the microscope objective lens 20 zoom the second direction of the exit spot, and therefore, changing the focal lengths of the second cylindrical lens 12 and the microscope objective lens 20 can change the zoom magnification of the second direction. Thus, the two directions of the illumination light spots can be respectively adjusted by changing the focal lengths of the first cylindrical lens 11 and the second cylindrical lens 12, and the shape and the size of the illumination light spots can be conveniently adjusted.
In the present embodiment, the shape of the illumination spot may be adjusted by adjusting the angle between the longitudinal direction of the first cylindrical lens 11 and the longitudinal direction of the second cylindrical lens 12, and it is preferable that the longitudinal direction of the first cylindrical lens 11 and the longitudinal direction of the second cylindrical lens 12 are perpendicular to each other. In this way, the first cylindrical lens 11 and the microscope objective lens 20 enlarge the length direction of the exit light spot, and the second cylindrical lens 12 and the microscope objective lens 20 reduce the width direction of the exit light spot to form a linear illumination light spot on the image plane.
The linear lighting device can output rectangular lighting spots matched with the detection surface of the camera by matching with the rectangular light spots output by the optical fibers 13, so that the light energy utilization rate can be improved.
Moreover, the first cylindrical lens 11 and the second cylindrical lens 12 are preferably arranged to be perpendicular to each other, and the first direction and the second direction of the exit light spot are respectively zoomed by different magnifications, so that a rectangular linear illumination light spot with good uniformity is obtained, and the shape of the illumination light spot is matched with the detection surface of the camera, so that the light energy utilization rate can be improved.
Moreover, the size of the illumination spot can also be adjusted by adjusting the first cylindrical lens 11 and the second cylindrical lens 12 by replacing the first cylindrical lens 11 and the second cylindrical lens 12 with different focal lengths. In some preferred embodiments, the first cylindrical lens 11 and the second cylindrical lens 12 having a large difference in focal length may be selected.
Illustratively, the core aperture of the optical fiber 13 may be selected to be 0.2mm ＊ 0.2.2 mm, and the numerical aperture NA may be 0.22, the focal length of the first cylindrical lens 11 is 1.56mm @550nm, the focal length of the second cylindrical lens 12 is 40mm @550nm, and the focal length of the microscope objective lens 20 is 12.5mm @550 nm.
Thus, the imaging magnification from the exit of the optical fiber 13 to the object plane of the microscope objective 20 is:
βx＝12.5/1.56≈8.01；
βy＝12.5/40≈0.31；
where β x is the magnification in the x-direction (i.e., the first direction) and β y is the magnification in the y-direction (i.e., the second direction).
The size of the illumination spot can be calculated according to the following object image magnification relation.
yl/y2＝f1/f2
Wherein y1 is the size of the exit spot and y2 is the size of the illumination spot; f1 is the focal length of the first cylindrical lens 11, and f2 is the focal length of the second cylindrical lens 12.
Therefore, the imaging size (i.e., the size in the first direction) of the first cylindrical lens 11 and the second cylindrical lens 12 is set to y2 being 0.2 ＊ 12.5.5/1.56 being 1.6026mm, and the length y' 2 (i.e., the size in the second direction) of the other side of the illumination spot is calculated to be 0.2 ＊ 12.5.5/40 being 0.0625 mm.
It can be seen that by varying the focal lengths of the first cylindrical lens 11 and the second cylindrical lens 12, rectangular illumination spots of different sizes can also be adjusted.
Fig. 2 shows the illumination spots imaged on the image plane by the linear illuminator. As shown in fig. 2, by adjusting the distance and angle between the optical fiber 13, the first cylindrical lens 11 and the second cylindrical lens 12, a rectangular illumination spot 30 as shown in fig. 2 can be imaged on the image plane 31, wherein the illumination spot 30 has a length of 1.6mm and a width of 62 um.
In some embodiments, the relative positions and angles between the optical fiber 13, the first cylindrical lens 11, and the second cylindrical lens 12 may be adjusted by providing structural members. Fig. 3 is a schematic structural view of the lighting module 10, and fig. 4 is a schematic sectional structural view taken along line IV-IV in fig. 3. As shown in fig. 3 and 4, the lighting module 10 further includes a base 14, a fiber mechanism 17, a first lens mechanism 16, and a second lens mechanism 15.
The base 14 is substantially cylindrical and includes a through hole formed therein to pass through both ends. The first lens mechanism 16 is used to connect the first cylindrical lens 11 to one end of the through hole of the base 14. The second lens mechanism 15 is used to connect the second cylindrical lens 12 to the other end of the through hole of the base 14. The optical fiber mechanism 17 is configured to connect the optical fiber to a side of the first cylindrical lens 11 away from the second cylindrical lens 12, so that the light emitted from the optical fiber 13 passes through the first cylindrical lens 11 and the second cylindrical lens 12 in sequence.
Fig. 5 is a schematic structural view of the base 14 and the first lens mechanism 16, and fig. 6 is a schematic sectional structural view of the base 14 and the first lens mechanism 16. As shown in fig. 5 and 6, the first lens mechanism 16 includes a first fixing member 161, a first lens adhesive member 162, and a first locking thread 163.
The first fixing member 161 is substantially cylindrical and is connected to an end portion of the base 14 in the axial direction of the base 14 such that an inner cavity of the first fixing member 161 is aligned and communicated with the through hole of the base 14. Referring to fig. 4 and 11 in detail, a rotatable ring 141 is provided inside the base 14. The rotating ring 141 has a ring shape. The first fixing member 161 has an external thread at one end for coupling with the internal thread of the rotating ring 141. Thus, the first fixing member 161 can be connected to the base 14 and can rotate relatively. After the relative position between the first fixing member 161 and the base 14 is adjusted, the screw thread connected between the first fixing member 161 and the rotating ring 141 is tightened, so as to fix the position of the first fixing member 161 relative to the base 14.
The first lens bonding member 162 is disposed around the first cylindrical lens 11, such that the first cylindrical lens 11 is located in the middle of the first lens bonding member 162 to fix the first cylindrical lens 11. The first lens bonding member 162 is movably coupled to the first fixing member 161 along the axial direction of the first fixing member 161 so that the first cylindrical lens 11 is aligned with the cavity of the first fixing member 161. When the first lens bonding piece 162 moves relative to the first fixing piece 161, the first lens bonding piece 162 drives the first cylindrical lens 11 to approach or depart from the second cylindrical lens 12, so that the relative distance, angle and position between the first cylindrical lens 11 and the second cylindrical lens 12 can be adjusted.
The first locking filament 163 penetrates the first fixing member 161 along the radial direction of the first lens adhesive piece 162 and abuts against the circumferential surface of the first lens adhesive piece 162 to lock the first lens adhesive piece 162. Therefore, when the first locking jack 163 is released, it is possible to move the first lens adhesive 162, adjust the radial position (the position of the plane perpendicular to the axis) of the first lens adhesive 162 within the first fixing member 161, and then lock the first locking jack 163 to fix the first lens adhesive 162. The first locking thread 163 is provided in plural, preferably two, and two first locking threads 163 are provided in mutually perpendicular directions. In order to provide a balance force equivalent to the two first locking jackscrews 163, two jackscrews are respectively arranged at the opposite ends of the two first locking jackscrews 163, and rubber rings 164 are arranged on the peripheries of the jackscrews to play a role in elastically compressing and providing a pre-tightening force.
Fig. 7 is a schematic structural view of the first fixing member 161 and the optical fiber mechanism 17, and fig. 8 is a schematic sectional structural view of the second fixing member 151 and the optical fiber mechanism 17. As shown in fig. 7 and 8, the optical fiber mechanism 17 includes an optical fiber connector 173, an optical fiber holder 171, and a second locking jack 172.
The fiber connector 173 includes a through hole (not shown) and is axially connected to the first fixture 161, and in this embodiment, the fiber connector 173 and the first fixture 161 are fixedly connected by screws. In this embodiment, the optical fiber connector 173 has a substantially cylindrical shape, and the through hole of the optical fiber connector 173 is aligned with the cavity of the first fixing member 161.
The optical fiber 13 is coupled to the middle of the optical fiber holder 171 to hold the optical fiber 13. The optical fiber holder 171 has a generally cylindrical shape, is movably coupled to the optical fiber connector 173 in an axial direction of the optical fiber connector 173, and has at least an end portion thereof located in the through hole of the optical fiber connector 173 such that the optical fiber holder 171 can move relative to the optical fiber connector 173. Referring to fig. 4, the first lens bonding member 162 is disposed on the optical fiber fixing member 171 and located between the first fixing member 161 and the optical fiber fixing member 171. When the optical fiber fixing member 171 moves relative to the optical fiber connector 173, the optical fiber fixing member 171 drives the optical fiber 13 to approach or separate from the first cylindrical lens 11. Referring to fig. 7 and 11, a handle 1711 is disposed at the end of the optical fiber fixing member 171, and a through hole is disposed on the handle 1711 and is connected to the optical fiber connecting member 173 by a screw and the through hole. The adjusting ring 174 is sleeved on the periphery of the lower end of the optical fiber fixing member 171, the adjusting ring 174 is provided with an internal thread, the periphery of the optical fiber fixing member 171 is provided with an external thread, and the optical fiber fixing member 171 is in threaded connection with the adjusting ring 174. The optical fiber fixing member 171 is adjusted to move the optical fiber 13 closer to or farther from the first cylindrical lens 11 by screwing the adjusting ring 174.
A plurality of second locking threads 172 pass through the circumferential surface of the optical fiber connector 173 in the radial direction of the optical fiber holder 171 and abut against the circumferential surface of the optical fiber holder 171 to lock the optical fiber holder 171. Therefore, when the second locking jack 172 is released, the optical fiber fixing member 171 may be moved, and then the second locking jack 172 may be locked to fix the optical fiber fixing member 171.
Fig. 9 is a schematic structural view of the base 14 and the second lens mechanism 15, and fig. 10 is a schematic sectional structural view of the base 14 and the second lens mechanism 15. As shown in fig. 9 and 10, the second lens mechanism 15 includes a second fixing member 151, a second lens bonding member 153, a connecting seat 152, and an adjusting screw 154.
The second fixing member 151 has a substantially cylindrical shape and a flange at the bottom. Which is captured in the through hole of the base 14. In this embodiment, the second fixing member 151 is connected to an end portion of the base 14 along the axial direction of the base 14 by a screw, and when the second fixing member 151 rotates relative to the base 14, the second fixing member 151 moves closer to or away from the base 14 along the axial direction of the through hole of the base 14. Referring to fig. 11, the second fixing member 151 is provided with an orientation groove 1511, an orientation pin 1411 is fixedly disposed on an inner wall of the rotating ring 141, and the orientation pin 1411 is located in the orientation groove 1511, so that the second fixing member 151 can drive the rotating ring 141 to rotate when rotating, and further drive the first fixing member 161 to rotate, and indirectly drive the first cylindrical lens 11 to rotate. The design is to ensure that the relative angle between the first cylindrical lens 11 and the second cylindrical lens 12 is unchanged and only the relative distance between the first cylindrical lens 11 and the second cylindrical lens 12 is changed when the second fixing member 151 rotates after the relative angle between the first cylindrical lens 11 and the second cylindrical lens 12 is adjusted.
The connection seat 152 is mounted to the second fixing member 151. In this embodiment, the connection holder 152 has a substantially cylindrical shape, and is fitted into an end portion of an inner cavity of the second fixing member 151 to be mounted in the second fixing member 151.
The adjusting screw 154 penetrates through the sidewall of the second fixing member 151 along the radial direction of the connection seat 152 and abuts against the circumferential surface of the connection seat 152. In this embodiment, the number of the adjusting screws 154 may be plural, and the adjusting screws are uniformly distributed in the first fixing member 161 along the circumferential direction. The position of the second cylindrical lens 12, that is, the radial relative position of the second cylindrical lens 12 and the first cylindrical lens 11 can be adjusted in the radial direction by adjusting the screwing length of the screw 154.
The second lens adhesive 153 is disposed around the circumference of the second cylindrical lens 12 to fix the second cylindrical lens 12 to the second lens adhesive 153. The second lens bonding member 153 is connected to the connecting seat 152 through a thread, and when the second lens bonding member 153 rotates relative to the second fixing member 151, the second lens bonding member 153 drives the second cylindrical lens 12 to approach or depart from the first cylindrical lens 11 and adjusts the angles of the first cylindrical lens 11 and the second cylindrical lens 12 in the length direction. In this embodiment, the end of the second lens adhesive 153 has two handles 1531 to rotate the second lens adhesive 153.
In operation, the first cylindrical lens 11 and the second cylindrical lens 12 with appropriate focal lengths may be selected, and the first cylindrical lens 11 is mounted to the first lens adhesive 162 and the second cylindrical lens 12 is mounted to the second lens adhesive 153. Further, the optical fiber 13 is installed into the fiber fixing member 171.
When adjusting the optical path of the linear lighting device, the following method can be adopted:
1. in adjusting the distance between the first cylindrical lens 11 and the second cylindrical lens 12, it is possible to:
A. the first lens adhesive member 162 is moved by loosening the screws of the first locking thread 163 and the knob 1711, rotating the adjustment ring 174 so that the first cylindrical lens 11 is close to or away from the second cylindrical lens 12, and after the adjustment is completed, the screws of the first locking thread 163 and the knob 1711 are locked to fix the first lens adhesive member 162.
B. The second lens bonding element 153 is rotated to make the second lens bonding element 153 rotate relative to the second fixing element 151, so as to drive the second cylindrical lens 12 to approach or depart from the first cylindrical lens 11.
C. The second fixing element 151 is rotated to rotate relative to the base 14, so as to adjust the second cylindrical lens 12 to approach or depart from the first cylindrical lens 11, but the relative angle between the two lenses is not affected.
2. Adjusting the distance between the optical fiber 13 and the first cylindrical lens 11: the second locking screw 172 and the screw on the knob 1711 are loosened, the adjustment ring 174 is rotated, and the optical fiber fixing member 171 is moved so that the optical fiber 13 is brought closer to or away from the first cylindrical lens 11.
3. Adjusting the radial position of the second cylindrical lens 12 with respect to the first cylindrical lens 11: the screwing length of each of the adjustment screws 154 is rotated so that the adjustment screw 154 abuts against the second lens adhesive member 153 and moves the second lens adhesive member 153 in the radial direction until the first cylindrical lens 11 and the second cylindrical lens 12 are adjusted to a predetermined relative position. It is also possible to rotate the screwing length of each first locking jackscrew 163 in a coordinated manner so that the first locking jackscrew 163 abuts against the first lens adhesive 162 and moves the first lens adhesive 162 in the radial direction until the first cylindrical lens 11 and the second cylindrical lens 12 are adjusted to predetermined relative positions.
4. Adjusting the relative angle between the first cylindrical lens 11 and the second cylindrical lens 12: the first lens adhesive member 162 or the second lens adhesive member 153 is rotated to adjust a relative angle between the length directions of the first cylindrical lens 11 and the second cylindrical lens 12 to adjust the shape of the illumination spot 30.
In addition, the present embodiment also provides a gene sequencer, which comprises the linear illumination device, wherein the illumination spot 30 is imaged on an image plane 31 by the light emitted by the linear illumination device. It should be noted that, within the scope of the spirit or the basic features of the present invention, each specific solution applicable to the first embodiment may also be correspondingly applicable to the second embodiment, and for the sake of brevity and avoidance of repetition, the detailed description thereof is omitted here.
In the several embodiments provided in the present invention, it should be understood that the disclosed components and structures may be implemented in other ways. It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned. Furthermore, it is to be understood that the word "comprising" does not exclude other elements or steps, and the singular does not exclude the plural. Several units or means recited in the system claims may also be implemented by one and the same unit or means in software or hardware. The terms first, second, etc. are used to denote names, but not any particular order.
Although the present invention has been described in detail with reference to the preferred embodiments, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (10)
1. A linear illumination device comprising an illumination module and a microscope objective for receiving light transmitted through the illumination module, the illumination module comprising:
the optical fiber is used for projecting outlet light spots;
the first cylindrical lens receives the outlet light spot projected by the optical fiber;
the second cylindrical lens receives light rays emitted by the first cylindrical lens and projects the outlet light spots on an image surface through the microscope objective to form illumination light spots, the first cylindrical lens and the microscope objective zoom the outlet light spots in a first direction, and the second cylindrical lens and the microscope objective zoom the outlet light spots in a second direction to form linear illumination light spots.
2. The line illuminator of claim 1, wherein the core of the optical fiber is rectangular in cross-section such that the exit spot is rectangular.
3. The line illuminator of claim 2, wherein the first cylindrical lens has a length direction perpendicular to a length direction of the second cylindrical lens, the first cylindrical lens and the microscope objective lens magnify the length direction of the exit spot, and the second cylindrical lens and the microscope objective lens demagnify the width direction of the exit spot to form a line illumination spot at the image plane.
4. The line illuminator of claim 1, wherein the first cylindrical lens is one of a single lens, a cemented lens, and a lens assembly; the second cylindrical lens is one of a single lens, a cemented lens and a lens group.
5. The line illuminator of claim 1, further comprising:
the base comprises a through hole which passes through two ends;
a first lens mechanism for connecting the first cylindrical lens to one end of the through hole of the base;
the second lens mechanism is used for connecting the second cylindrical lens to the other end of the through hole of the base;
and the optical fiber mechanism is used for connecting the optical fiber to one side of the first cylindrical lens, which is far away from the second cylindrical lens, so that the light rays emitted from the optical fiber sequentially pass through the first cylindrical lens and the second cylindrical lens.
6. The line illuminator of claim 5, wherein the first lens mechanism comprises:
the first fixing piece is connected to the end part of the base along the axial direction of the base;
the first lens bonding piece is sleeved on the peripheral surface of the first cylindrical lens and is movably connected to the first fixing piece along the axial direction of the first fixing piece, and when the first lens bonding piece moves relative to the first fixing piece, the first lens bonding piece is close to or far away from the second cylindrical lens;
and the first locking jackscrew penetrates through the first fixing piece along the radial direction of the first lens bonding piece and abuts against the peripheral surface of the first lens bonding piece so as to lock the first lens bonding piece.
7. The line illuminator of claim 6, wherein the fiber optic mechanism comprises:
the optical fiber connector comprises a through hole and is connected to the first fixing piece along the axial direction;
the optical fiber fixing piece is connected with the optical fiber in the middle, movably connected with the optical fiber connecting piece along the axial direction of the optical fiber connecting piece, and at least arranged in the through hole of the optical fiber connecting piece at the end part, and drives the optical fiber to be close to or far away from the first cylindrical lens when the optical fiber fixing piece moves relative to the optical fiber connecting piece;
and the second locking jackscrew penetrates through the peripheral surface of the optical fiber connector along the radial direction of the optical fiber fixing piece and abuts against the peripheral surface of the optical fiber fixing piece so as to lock the optical fiber fixing piece.
8. The line illuminator of claim 5, wherein the second lens mechanism comprises:
the second fixing piece is connected to the end part of the base along the axial direction of the base through threads, and when the second fixing piece rotates relative to the base, the second fixing piece is close to or far away from the first cylindrical lens;
the second lens bonding piece is sleeved on the circumferential surface of the second cylindrical lens and is in threaded connection with the second fixing piece, and the second lens bonding piece is opposite to the second fixing piece and drives the second cylindrical lens to be close to or far away from the first cylindrical lens when the second fixing piece rotates.
9. The line illuminator of claim 8, wherein the second lens mechanism further comprises:
the connecting seat is arranged on the second fixing piece, and the second lens bonding piece is connected to the connecting seat through threads;
and the adjusting screw penetrates through the second fixing piece along the radial direction of the connecting seat and abuts against the peripheral surface of the connecting seat so as to adjust the position of the second cylindrical lens along the radial direction.
10. A gene sequencer comprising the line illuminator of any of claims 1-9, wherein the light from the line illuminator forms an illumination spot on an image plane.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811515785.0A CN111308680B (en) | 2018-12-11 | 2018-12-11 | Linear lighting device and gene sequencer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811515785.0A CN111308680B (en) | 2018-12-11 | 2018-12-11 | Linear lighting device and gene sequencer |
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| CN111308680A true CN111308680A (en) | 2020-06-19 |
| CN111308680B CN111308680B (en) | 2022-09-30 |
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| CN113552677A (en) * | 2021-07-28 | 2021-10-26 | 上海索迪龙自动化有限公司 | Optical fiber transmitting port |
| CN113759561A (en) * | 2021-08-19 | 2021-12-07 | 深圳赛陆医疗科技有限公司 | Light shaping homogenization assembly, laser lighting device and gene sequencing system |
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| CN108572439A (en) * | 2017-03-08 | 2018-09-25 | 伊鲁米那股份有限公司 | Laser rays luminaire for high-flux sequence |
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| CN113759561A (en) * | 2021-08-19 | 2021-12-07 | 深圳赛陆医疗科技有限公司 | Light shaping homogenization assembly, laser lighting device and gene sequencing system |
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| CN111308680B (en) | 2022-09-30 |
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