CN113759561A

CN113759561A - Light shaping homogenization assembly, laser lighting device and gene sequencing system

Info

Publication number: CN113759561A
Application number: CN202110953561.3A
Authority: CN
Inventors: 梁倩; 陈龙超; 王谷丰
Original assignee: Shenzhen Sailu Medical Technology Co ltd
Current assignee: Shenzhen Sailu Medical Technology Co ltd
Priority date: 2021-08-19
Filing date: 2021-08-19
Publication date: 2021-12-07

Abstract

The invention discloses a light shaping and homogenizing component, which comprises: the device comprises a collimating optical element, a light evening element and a light condensing element with adjustable focal length, wherein incident light is collimated into first light after entering the collimating optical element, the first light is homogenized by the light evening element to form second light, and the second light is condensed by the light condensing element to form target light. The invention also discloses a laser lighting device and a gene sequencing system. The invention at least solves the technical problems of improving the light uniformity and adjusting the spot size in the light shaping process.

Description

Light shaping homogenization assembly, laser lighting device and gene sequencing system

Technical Field

The invention relates to the technical field of optics. More particularly, the invention relates to a light shaping and homogenizing assembly, a laser illumination device and a gene sequencing system.

Background

The gene sequencer needs to use a laser light source to illuminate a sample to be tested, and the laser light source output by a light excitation device (such as a laser) is generally Gaussian or ultra-Gaussian distributed light, namely the light intensity distribution of the center and the edge of the light is not uniform. Traditional gene sequencer usually adopts the mode of cylindrical mirror plastic light to become linear light with laser light source plastic, but, the homogeneity of illumination facula can't be changed to cylindrical mirror plastic mode, to the two-dimensional light of gauss or super high gauss distribution, through the cylindrical mirror to one-dimensional direction plastic compression back, central light intensity can be far away from marginal light intensity, still has the inhomogeneous defect of light.

The traditional gene sequencer also adopts a mode of adjusting the intensity of incident light to improve the integral intensity of the incident light so as to improve the signal-to-noise ratio of a sample at the edge of a visual field. However, the solution causes the light central intensity to exceed a certain limit, and the light absorption tends to be saturated, thereby causing phototoxicity and causing photo-bleaching of the sample to be detected.

Disclosure of Invention

It is an object of the present invention to at least solve the above problems and to provide corresponding advantages.

Another objective of the present invention is to provide a light-shaping homogenizing assembly, a laser illumination device and a gene sequencing system, which at least solve the technical problems of improving light uniformity and adjusting spot size during light shaping. The invention is mainly realized by the technical scheme in the following aspects:

< first aspect of the invention >

A first aspect provides a light shaping and homogenizing assembly, comprising:

a collimating optical element, a dodging element, and a focus adjustable condensing element, wherein,

incident light is collimated into first light after entering the collimating optical element, the first light is homogenized by the light homogenizing element to form second light, and the second light is condensed by the light condensing element to form target light.

In the first aspect of the present invention, the shaping and homogenizing process may be performed on the incident light output from the optical excitation device. Specifically, the incident light is collimated into the two-dimensional first light. And then homogenizing the first light ray, so that the uniformity of the first light ray is greatly improved. And finally, gathering the homogenized light rays into one-dimensional target light rays. Therefore, the technical scheme provided by the invention can eliminate the central hot spot and the discolored edge distribution of the Gaussian or ultrahigh Gaussian distribution light source in the light shaping and homogenizing process, greatly improve the uniformity of light and avoid the phenomenon of generating low signal-to-noise ratio or causing phototoxicity.

Moreover, when the technical scheme is applied to light shaping and homogenizing, one-dimensional target light is converged on an imaging surface of the condensing element through the condensing element, and on the basis, the spot size of the target light can be adjusted by adjusting the focal length of the condensing element, so that the method can adapt to more gene sequencing scenes.

In some embodiments, the collimating optical element is configured as a double cemented lens.

In some embodiments, the double cemented lens is formed by a meniscus lens cemented with a double convex lens.

In some aspects, the collimating optics are also used to correct chromatic aberration.

In some embodiments, the light homogenizing element is configured as a powell prism.

In some embodiments, the dodging element is further configured to adjust the spot size.

In some embodiments, the collimating optical element and the homogenizing element are further configured to adjust the spot size.

In some embodiments, the light-collecting element is configured as an objective lens.

< second aspect of the invention >

A second aspect provides a laser lighting device comprising:

the light shaping and homogenizing assembly of the first aspect;

a laser; and

and the laser coupler is used for coupling a laser light source emitted by the laser into incident light of the light shaping and homogenizing component.

< third aspect of the invention >

A third aspect provides a gene sequencing system comprising:

a gene sequencer; and

the laser illumination device of the second aspect is used for providing an illumination light source for the gene sequencer.

The embodiment of the invention has at least the following beneficial effects:

the invention can carry out shaping homogenization treatment on the incident light output by the light excitation equipment. Specifically, the incident light is collimated into the two-dimensional first light. And then homogenizing the first light ray, so that the uniformity of the first light ray is greatly improved. And finally, gathering the homogenized light rays into one-dimensional target light rays. Therefore, the technical scheme provided by the invention can eliminate the central hot spot and the discolored edge distribution of Gaussian or ultrahigh-Gaussian distributed light in the light shaping and homogenizing process, greatly improve the uniformity of the light and avoid the phenomenon of generating low signal-to-noise ratio or causing phototoxicity.

Moreover, when the technical scheme is applied to light shaping and homogenizing, one-dimensional target light is converged on an imaging surface of the light condensing element, and based on the one-dimensional target light, the spot size of the target light can be adjusted by adjusting the focal length of the light condensing element, so that the method can adapt to more gene sequencing scenes.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the description of the embodiments of the present application will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without inventive exercise.

FIG. 1 is a schematic view of a light shaping and homogenizing assembly of the present invention in some embodiments;

FIG. 2 is a view of an illumination spot imaged at an image plane by the light shaping and homogenizing assembly of the present invention in some embodiments;

FIG. 3 is a graph of the uniformity of the illumination spot for the target light of the present invention;

FIG. 4 is a block diagram of a laser illumination device of the present invention in some embodiments;

FIG. 5 is a block diagram of a gene sequencing system of the invention in some embodiments;

description of reference numerals:

10. a light shaping and homogenizing component;

11. a collimating optical element;

12. a light uniformizing element;

13. a light condensing element;

14. incident light; 141. a first light ray; 142. a second light ray; 143. a target ray; 1431. illuminating the light spot;

20. a laser illumination device;

21. a laser;

22. a laser coupler;

30. a gene sequencing system;

31. a gene sequencer.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more clearly and completely apparent, the technical solutions in the embodiments of the present application will be described below in conjunction with the drawings in the embodiments of the present application, and it is obvious that the described embodiments are some embodiments of the present application, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

In the embodiments of the present application, words such as "exemplary" or "for example" are used to mean serving as an example, instance, or illustration. Any embodiment or design described herein as "exemplary" or "e.g.," is not necessarily to be construed as preferred or advantageous over other embodiments or designs. Rather, use of the word "exemplary" or "such as" is intended to present concepts related in a concrete fashion.

It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements explicitly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.

In addition to the foregoing, it remains emphasized that reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the present application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.

For a better understanding of the technical solutions provided by the present invention, the terms appearing herein are explained accordingly:

two-dimensional: referring to the content on one plane, in this context, the "two-dimensional first light ray" may be understood as that the light spots of the first light ray are all non-linear plane light spots, and may be circular, square or other shapes.

One-dimensional: refers to a line. In this context, "one-dimensional target light" is understood to mean that the spots in the target light are all linear spots.

< light shaping and homogenizing Assembly >

Referring to FIG. 1, there is shown a schematic view of a light shaping and homogenizing assembly 10 according to some embodiments of the present invention. In fig. 1, the light beam shaping and homogenizing assembly 10 includes a collimating optical element 11, a light homogenizing element 12, and a light condensing element 13 with adjustable focal length, wherein an incident light 14 enters the collimating optical element 11 and is collimated into a first light beam 141, the first light beam 141 is shaped and homogenized by the light homogenizing element 12 to form a second light beam 142, and the second light beam 142 is condensed by the light condensing element 13 to form a target light beam 143.

The collimating optical element 11, the dodging element 12 and the condensing element 13 may be arranged in sequence along an incident direction of the incident light 14. The specific arrangement order can be seen with reference to fig. 1.

The incident light 14 may be a light beam containing multiple bands or a light beam containing a single band. Illustratively, the incident light 14 may include two-band light of 532nm and 640nm bands, or may be single-band light of 532 nm. The light of this application is used mainly for the illumination to wait to detect the sample, therefore, can confirm the wave band of light according to the actual demand by the technical staff in the art, and this application does not limit this.

An illumination spot 1431 of the target light ray 143 imaged on the image plane can be shown in fig. 2. Through optical design and simulation software simulation, the energy distribution uniformity of the illumination spot 1431 in the center of the short side along the long side direction in fig. 2 can be referred to as fig. 3.

It should be noted that fig. 2 is an exemplary illumination spot 1431 output by using the optical design and simulation software Zemax, and in fig. 2, the abscissa is the spot length along the long side direction of the illumination spot 1431, and the ordinate is the spot width along the short side direction of the illumination spot 1431.

Fig. 3 shows the result of intensity uniformity in the long side direction at the center of the short side of the illumination spot 1431, with the abscissa being the spot length in the long side direction of the illumination spot 1431 and the ordinate being the spot intensity.

By the technical scheme, the incident light 14 output by the optical excitation device can be shaped and homogenized. Specifically, the incident light 14 is collimated into the two-dimensional first light ray 141. The first light beam 141 is then homogenized, so that the uniformity of the first light beam 141 is greatly improved. Finally, the homogenized light is converged into a one-dimensional target light 143. Therefore, the technical scheme provided by the invention can eliminate the central hot spot and the discolored edge distribution of Gaussian or ultrahigh Gaussian distribution light in the light shaping and homogenizing process, greatly improve the uniformity of the target light along the long edge direction, and avoid the phenomenon of generating low signal-to-noise ratio or causing phototoxicity.

Moreover, when the technical scheme is applied to light shaping and homogenizing, the one-dimensional target light 143 is converged on the imaging surface of the light condensing element 13, and based on the convergence, the spot size of the target light 143 can be adjusted by adjusting the focal length of the light condensing element 13, so that the method can adapt to more gene sequencing scenes.

Further, the light shaping and homogenizing assembly 10 can be applied to an epi-illumination device, can enable an illumination light path and an imaging light path to be in the same direction, and is suitable for non-transparent objects such as metals, minerals and the like.

In some embodiments, the collimating optical element 11 is provided as a double cemented lens. The double cemented lens may be cemented by a meniscus lens and a double convex lens. The design parameters corresponding to the light incident surface, the bonding surface and the light emergent surface of the double cemented lens can be implemented by referring to table 1. After the incident light 14 enters the collimating optical element 11, the collimating optical element 11 collimates the incident light 14 into parallel light, i.e. a first light ray 141.

In some embodiments, the collimating optical element 11 is also used to correct chromatic aberrations. When the incident light 14 is a light beam containing multiple bands, the collimating optical element 11 needs to correct the chromatic aberration of the incident light 14.

In some embodiments, the light homogenizing element 12 is configured as a powell prism. The design parameters of the light incident surface and the light emergent surface of the powell prism can be implemented by referring to table 1. The Powell prism homogenizes the first light 141 to form a second light 142. After the second light 142 exits from the powell prism light-emitting surface, the second light is gradually converged and diffused toward a focus corresponding to the powell prism, and the diffused second light 142 enters the light-condensing element 13. It should be understood that the focal point corresponding to the powell prism is disposed close to the light incident surface of the light condensing element 13, and the specific distance is set by those skilled in the art according to actual requirements.

In some embodiments, the light homogenizing element 12 is also used to adjust the spot size. Specifically, the spot size can be adjusted by changing the radius of curvature and the cone coefficient of the light uniformizing element 12.

In some embodiments, the collimating optical element 11 and the homogenizing element 12 are also used to adjust the spot size. In particular, the spot size can be adjusted by changing the focal length of the collimating optical element 11, the radius of curvature of the light unifying element 12, and the conic coefficient of the light unifying element 12.

In some embodiments, the light-condensing element 13 is provided as an objective lens. The objective lens is used for focusing the second light 142 into target light 143, and irradiating the target light 143 onto a corresponding gene sequencing chip.

In some embodiments, the parameter designs of the collimating optical element 11, the dodging element 12 and the condensing element 13 may be as shown in table 1.

TABLE 1

Numbering	Radius of curvature/mm	Thickness/mm	Refractive index	Abbe number	Coefficient of cone	Focal length
							1	247.2	2	1.8052	25.36	-	-
2	46.4	16	1.67	47.112	-	-
							3	-54.9	30	-	-	-	-
4	82	15	1.5168	64.167	-26	-
							5	Plane surface	219.221	-	-	-	-
6	-	10	-	-	-	10

In table 1, the parameter information corresponding to the number 1 is the parameter information of the light incident surface of the collimating optical element 11; the parameter information corresponding to the number 2 is the parameter information of the gluing surface of the collimating optical element 11; the parameter information corresponding to the number 3 is the parameter information of the light-emitting surface of the collimating optical element 11; the parameter information corresponding to the number 4 is the parameter information of the light incident surface of the light uniformizing element 12; the parameter information corresponding to the number 5 is the parameter information of the light-emitting surface of the dodging element 12; the parameter information corresponding to the number 6 is the parameter information of the light condensing element 13.

In addition to the above listed parameter information, it should be understood by those skilled in the art that the parameter information of the collimating optical element 11, the dodging element 12 and the condensing element 13 may have other matching schemes in terms of optical design, and is not limited to the parameter information shown in table 1.

< laser illumination apparatus >

As shown in fig. 4, a block diagram of a laser illumination device 20 of the present invention is shown in some embodiments. In fig. 4, the laser illumination device 20 includes:

the light shaping and homogenizing assembly 10 of the first aspect;

a laser 21;

and the laser coupler 22 is used for coupling a laser light source emitted by the laser into incident light of the light shaping and homogenizing assembly.

In some embodiments, the laser coupler 22 is also used to homogenize the incident light.

< Gene sequencing System >

FIG. 5 is a block diagram of a gene sequencing system 30 of the present invention in some embodiments. In fig. 5, the gene sequencing system 30 includes:

a gene sequencer 31; and

the laser illumination device 20 of the second aspect, configured to provide a light source for the gene sequencer.

While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details shown and described herein without departing from the general concept defined by the claims and their equivalents.

Claims

1. A light shaping and homogenizing assembly, comprising:

2. The light-shaping homogenizing assembly of claim 1 wherein the collimating optics are configured as a double cemented lens.

3. The light shaping homogenizing assembly of claim 2 wherein the double cemented lens is formed by a meniscus lens cemented with a double convex lens.

4. The light shaping homogenizing assembly of claim 2 wherein the collimating optics are further configured to correct chromatic aberrations.

5. The light shaping homogenizing assembly of claim 1 wherein the light homogenizing element is configured as a Powell prism.

6. The light shaping homogenizing assembly of claim 5 wherein the light homogenizing element is further configured to adjust spot size.

7. The light shaping homogenizing assembly of claim 1 wherein the collimating optics and the homogenizing elements are further configured to adjust spot size.

8. The light shaping homogenizing assembly of claim 1 wherein the light condensing element is configured as an objective lens.

9. Laser lighting device, characterized by, includes:

the light shaping homogenizing assembly of any one of claims 1-8;

a laser; and

10. A gene sequencing system, comprising:

a gene sequencer; and

the laser illumination device of claim 9, configured to provide a light source for the gene sequencer.