CN111296423A - Insecticidal composition containing saw-toothed diatomite and chamaejasmine and application thereof - Google Patents
Insecticidal composition containing saw-toothed diatomite and chamaejasmine and application thereof Download PDFInfo
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- CN111296423A CN111296423A CN202010110401.8A CN202010110401A CN111296423A CN 111296423 A CN111296423 A CN 111296423A CN 202010110401 A CN202010110401 A CN 202010110401A CN 111296423 A CN111296423 A CN 111296423A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/08—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing solids as carriers or diluents
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/12—Powders or granules
- A01N25/14—Powders or granules wettable
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/30—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests characterised by the surfactants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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Abstract
The invention relates to the technical field of pesticides, and particularly relates to an insecticidal composition containing saw-toothed diatomite and chamaejasmine and application thereof. The preparation raw materials of the insecticidal composition comprise saw-tooth diatomite and chamaejasmine in a weight ratio of 200: 1-1: 40. the applicant finds that the control effect and efficiency of various plant pests can be improved by compounding the sawtooth-shaped diatomite and the chamaejasmine, and the compound pesticide has a good insecticidal effect on various insects such as thrips occidentalis, whitefly, liriomyza sativae, prodenia litura, tea lesser leafhopper, tea thrips flavivis, tea geometrid, green plant bug, grape aphid and the like; in addition, the applicants have found that by selecting suitable fillers and dispersants, the wetting and dispersing properties of the pesticidal composition can be increased and that the composition has high storage stability when the formulation is a wettable powder or a water dispersible granule.
Description
Technical Field
The invention relates to the technical field of pesticides, and particularly relates to an insecticidal composition containing saw-toothed diatomite and chamaejasmine and application thereof.
Background
Most of the insecticides currently used in China are chemical products, and the use of the chemical pesticides on crops easily causes pesticide residues, thereby forming certain threats to the health of people. Therefore, biological pesticides are urgently needed for the production of green and healthy foods, and new high-efficiency and safe biological pesticide varieties and new formulations are vigorously developed.
The stellera chamaejasme toxin is obtained by extracting and separating stellera chamaejasme, has good activities of food refusal, contact killing activity, growth inhibition and the like on pests, and can effectively prevent and control homoptera, lepidoptera and acarina pests such as aphids, stem borers, cabbage caterpillars, mites and the like. And the chamaejasmine is a natural extract, is safe to plants without phytotoxicity, is a high-efficiency and low-residue biological insecticide with high safety, and is suitable for vegetables, fruits and gardening.
In addition, besides biological insecticides, physical insecticides such as diatomite are widely concerned at present, have the advantages of good insect prevention and killing effect, long protection period, no toxicity to mammals, no environmental pollution and the like, accord with the development direction of green stored grains, and are favored by many national insect control experts. However, the diatomite pesticide is in the beginning stage in China at present, and due to the nature of diatomite, the pesticide is poor in dispersion and wettability. The replacement of chemical pesticides by combining physical and biological pesticides is currently a focus.
Disclosure of Invention
In order to solve the above problems, the first aspect of the present invention provides an insecticidal composition comprising saw-toothed diatomaceous earth and chamaejasmine, wherein the preparation raw materials of the insecticidal composition comprise the saw-toothed diatomaceous earth and the chamaejasmine in a weight ratio of 200: 1-1: 40.
as a preferable technical scheme of the invention, the preparation raw materials of the insecticidal composition further comprise a filler, and the filler is one or more selected from kaolin, white carbon black, magnesium aluminum silicate, anhydrous sodium sulfate, glucose, pottery clay, sericite, fructose and soluble starch.
As a preferable technical scheme, the filler accounts for 5-10 wt% of the total weight of the sawtooth-shaped diatomite and the chamaejasmine.
As a preferable technical scheme, the filler comprises white carbon black, and the specific surface area of the white carbon black is 300-500 m2/g。
As a preferable technical scheme of the invention, the filler further comprises magnesium aluminum silicate, and the weight ratio of the white carbon black to the magnesium aluminum silicate is 1: (0.5 to 1.5).
As a preferable technical scheme of the invention, the preparation raw material of the insecticidal composition further comprises a dispersant, and the dispersant is selected from one or more of alkylphenol polyoxyethylene phosphate, fatty acid polyoxyethylene ether, alkyl naphthalene sulfonate, polycarboxylate, dodecyl sulfate, phosphate ester salt, lignosulfonate, alkylphenol polyoxyethylene ether and alkylphenol formaldehyde resin polyoxyethylene ether.
As a preferable technical scheme, the dispersing agent accounts for 4-8 wt% of the total weight of the sawtooth-shaped diatomite and the chamaejasmine.
In a second aspect, the invention provides a wettable powder, which is prepared from the raw materials of the insecticidal composition containing the saw-toothed diatomite and the chamaejasmine as described above.
The invention provides a water dispersible granule, which is prepared from the raw materials of the water dispersible granule, wherein the raw materials comprise the insecticidal composition containing the saw-toothed diatomite and the chamaejasmine.
The fourth aspect of the invention provides the application of the insecticidal composition containing the saw-toothed diatomite and the chamaejasmine in the prevention and control of plant pests.
Compared with the prior art, the invention has the following beneficial effects: the applicant finds that the control effect and efficiency of various plant pests can be improved by compounding the zigzag diatomite and the chamaejasmine, and particularly the control effect on various insects such as thrips occidentalis, whitefly, liriomyza sativae, prodenia litura, tea lesser leafhopper, tea thrips flavivis, tea geometrid, green plant bug, grape aphid and the like is good; in addition, the applicants have found that by selecting suitable fillers and dispersing agents, the wetting and dispersing properties of the pesticidal composition can be increased, and that when the formulation of the composition is a wettable powder or a water dispersible granule, it has high storage stability.
Drawings
Fig. 1 is an optical microscope image of a serrated diatomaceous earth Celite 610.
FIG. 2 is an electron microscope image of a sawtooth-shaped diatomaceous earth Celite 610.
Detailed Description
The disclosure may be understood more readily by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. In case of conflict, the present specification, including definitions, will control.
The term "prepared from …" as used herein is synonymous with "comprising". The terms "comprises," "comprising," "includes," "including," "has," "having," "contains," "containing," or any other variation thereof, as used herein, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises a list of elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.
The conjunction "consisting of …" excludes any unspecified elements, steps or components. If used in the claims, this phrase will cause the claims to be closed, containing no material other than those described, except for the usual impurities associated therewith. When the phrase "consisting of …" appears in a clause of the subject matter of the claims rather than immediately after the subject matter, it defines only the elements described in the clause; other elements are not excluded from the claims as a whole.
When an amount, concentration, or other value or parameter is expressed as a range, preferred range, or as a range of values, with a range of upper preferable values and lower preferable values, this is to be understood as specifically disclosing all ranges formed from any pair of any upper range limit or preferred value and any lower range limit or preferred value, regardless of whether ranges are separately disclosed. For example, when a range of "1 to 5" is disclosed, the described range should be interpreted to include the ranges "1 to 4", "1 to 3", "1 to 2 and 4 to 5", "1 to 3 and 5", and the like. When a range of values is described herein, unless otherwise stated, the range is intended to include the endpoints thereof and all integers and fractions within the range.
The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. "optional" or "either" means that the subsequently described event or events may or may not occur, and that the description includes instances where the event occurs and instances where it does not.
Approximating language, as used herein in the specification and claims, is intended to modify a quantity, such that the invention is not limited to the specific quantity, but includes equivalents thereof that are acceptable without resulting in a change in the basic function to which it is related. Accordingly, a value modified by "about" or "approximately" is intended to mean that the invention is not limited to the precise value. In some instances, the approximating language may correspond to the precision of an instrument for measuring the value. In the present description and claims, range limitations may be combined and/or interchanged, including all sub-ranges contained therein if not otherwise stated.
In addition, the indefinite articles "a" and "an" preceding an element or component of the invention are not intended to limit the number requirement (i.e., the number of occurrences) of the element or component. Thus, "a" or "an" should be read to include one or at least one, and the singular form of an element or component also includes the plural unless the stated number clearly indicates that the singular form is intended.
The present invention is illustrated by the following specific embodiments, but is not limited to the specific examples given below.
The invention provides a desinsection composite containing saw-tooth diatomite and chamaejasmine, which is prepared from the following raw materials in parts by weight of 200: 1-1: 40.
in the present invention, the term "composition" means that the components of the composition are packaged in combination or separately and used together when used. That is, in the invention, the 'saw-toothed diatomite and chamaejasmine' means that the saw-toothed diatomite and the chamaejasmine can be mixed and packaged together and used together when in use; also comprises a package of the sawtooth-shaped diatomite and the chamaejasmine separately, and the sawtooth-shaped diatomite and the chamaejasmine are mixed together according to a proportion when in use.
The applicant has found that when the crenellated diatomaceous earth and chamaejasmine according to the invention are compounded, the weight ratio is, in particular, 200: 1-1: 40, and the weight ratio of the saw-toothed diatomite to the chamaejasmine is 200: 1. 100, and (2) a step of: 1. 1: 1. 1: 20. 1: 40.
the sawtooth-shaped diatomite has a larger specific surface area and a sawtooth-shaped structure, is one of inert powder, is easier to adsorb on the surface of pests due to the sawtooth-shaped structure of the sawtooth-shaped diatomite and damages wax layers on the epidermis parts of the pests, so that the water loss in the pests is excessive, and the sawtooth-shaped diatomite has the advantages of good insect prevention and killing effect, long protection period, no toxicity to mammals, no environmental pollution and the like. The saw-toothed diatomite of the present invention is available by self-made or commercially-available, and in a preferred embodiment, the saw-toothed diatomite of the present invention is obtained from Celite610 of yirui stone, france, and the saw-toothed structure thereof can be observed by an optical microscope and an electron microscope, as shown in fig. 1 and 2, and the saw-toothed honeycomb structure of the diatomite can be seen.
The stellera chamaejasme toxin is obtained by extracting and separating stellera chamaejasme, has good activities of food refusal, contact killing activity, growth inhibition and the like on pests, and can effectively prevent and control homoptera, lepidoptera and acarina pests such as aphids, stem borers, cabbage caterpillars, mites and the like. And the chamaejasmine is a natural extract, is safe to plants without phytotoxicity, is a high-efficiency and low-residue biological insecticide with high safety, and is suitable for vegetables, fruits and gardening. The chamaejasmine of the present invention may be purchased or self-made, and in a preferred embodiment, is purchased from shanjincheng honest biotechnology limited, shanghai.
The applicant has unexpectedly found that the insecticidal effect of the composition of the present invention can be promoted by using the interaction between the saw-toothed diatomaceous earth and the chamaejasmine, probably because the adsorption to the insect surface can be increased by using the saw-toothed diatomaceous earth, thereby promoting the contact killing efficiency of the chamaejasmine, and the saw-toothed diatomaceous earth can absorb the moisture of the insect and destroy the body surface structure of the insect, thereby improving the insecticidal effect; in addition, the chamaejasmine and the saw-tooth-shaped diatomite can enter the insects together, the growth inhibition effect of the chamaejasmine on the insects is exerted, and the insecticidal effect is further promoted. And as a natural extract, the chamaejasmine is safe and harmless to plants, does not harm the plants together with the saw-tooth-shaped diatomite, and can have good insecticidal effect on various insects such as frankliniella occidentalis, trialeurodes vaporariorum, liriomyza sativae, prodenia litura, tea leafhopper, tea leaf thrips fulvidraco, tea leaf tea geometrid, green plant bug, grape aphid and the like.
In one embodiment, the raw materials for preparing the insecticidal composition according to the invention further comprise a filler.
Filler material
In one embodiment, the filler of the present invention is selected from one or more of kaolin, white carbon, magnesium aluminum silicate, anhydrous sodium sulfate, glucose, china clay, sericite, fructose, and soluble starch.
Preferably, the filler accounts for 5-10 wt% of the total weight of the sawtooth-shaped diatomite and the chamaejasmine; further, the filler of the invention accounts for 8 wt% of the total weight of the sawtooth-shaped diatomite and the chamaejasmine.
More preferably, the filler of the present invention comprises silica.
The white carbon black is white powdery X-ray amorphous silicic acid and silicate, and mainly refers to precipitated silica, fumed silica and superfine silica gel. White carbon black is a porous substance, and the composition of the white carbon black can be represented by SiO2 & nH2O, wherein nH2O exists in the form of surface hydroxyl. High-temperature resistance, non-combustion, tastelessness, odorless and good electrical insulation. The white carbon black can be purchased or manufactured by self, and in a preferred embodiment, the white carbon black is purchased from Wake HDKV15, HDK N20, HDK T30, HDK T40, HDK H15, HDK H20 and HDK H17One or more of HDK H18, HDK H30 and HDK H2000; in a more preferred embodiment, the white carbon black of the present invention is obtained from HDK V15 (specific surface area 150 m)2(g), HDK N20 (specific surface area 200 m)2(g), HDK T30 (specific surface area 300 m)2/g), HDK T40 (specific surface area 400 m)2One or more of the following components/g).
Further preferably, the specific surface area of the white carbon black is 200-400 m2(ii)/g; further, the specific surface area of the white carbon black is 200-300 m2(ii)/g; further, the specific surface area of the white carbon black is 300m2/g。
The specific surface area is the total area of the material per unit mass, and the unit is m2(ii) in terms of/g. The specific surface area of the present invention is measured according to a method well known in the art, and a continuous flow method and a static capacity method are enumerated.
The applicant finds that by adding white carbon black with large specific surface area as a filler, due to the large surface area and the porous structure of the white carbon black, when the composition disclosed by the invention is mixed with water and acts with a plant surface, the hydrophilic structure of polyhydroxy group on the surface of the white carbon black and the large surface area promote wetting and dispersion of the saw-tooth-shaped diatomite and the chamaejasmine on the plant surface, so that the wetting performance of the composition is improved, and the high specific surface area of the white carbon black is beneficial for the saw-tooth-shaped diatomite and the chamaejasmine to adsorb the plant surface, so that the loss of the saw-tooth-shaped diatomite and the chamaejasmine is avoided.
Still more preferably, the filler of the present invention further comprises magnesium aluminum silicate, and the weight ratio of white carbon black to magnesium aluminum silicate is 1: (0.5 to 1.5); further, the weight ratio of the white carbon black to the magnesium aluminum silicate is 1: (0.5 to 1); further, the weight ratio of the white carbon black to the magnesium aluminum silicate is 1: 0.6.
the aluminum magnesium silicate has a unique three-dimensional chain structure and a special needle and rod crystal structure, so that the aluminum magnesium silicate has unusual colloid and adsorption properties. The material is widely applied to various fields by virtue of excellent thickening property, suspension property and colloid shaking and melting property, and forms a 'card palace' type association network structure in an aqueous medium. The magnesium aluminum silicates of the present invention are commercially or self-made, and in a preferred embodiment are purchased from one or more of r.t. vanderbilt's magnesium aluminum silicate HV, magnesium aluminum silicate K, magnesium aluminum silicate F, magnesium aluminum silicate NG, magnesium aluminum silicate T, magnesium aluminum silicate HS, magnesium aluminum silicate PRO (organic cation modified magnesium aluminum silicate); in a more preferred embodiment, the magnesium aluminum silicate of the present invention is magnesium aluminum silicate PRO.
In a preferred embodiment, the magnesium aluminum silicate of the present invention is an organic cation-modified magnesium aluminum silicate.
The organic cation modified magnesium aluminum silicate is chemically modified, and exchangeable sodium ions in the magnesium aluminum silicate are replaced by existing cations, so that the magnesium aluminum silicate has good tolerance to organic solvents and electrolytes.
The applicant has unexpectedly found that the suspension and wetting properties of the composition can be improved by adding magnesium aluminum silicate, especially organic cation modified magnesium aluminum silicate and white carbon black, and when the composition is in the form of water dispersible granule, the hot-pressing stability of the composition can also be improved, probably because magnesium aluminum silicate rapidly absorbs water and swells in water to form a unique three-dimensional chain structure, and organic cation can react with white carbon black and sawtooth-shaped diatomite to participate in the formation of a physical cross-linking network to increase the viscosity of the aqueous solution, thereby promoting the suspension of the composition particles in water, and because the strong water absorption of magnesium aluminum silicate can promote the dehydration of insects together with diatomite to improve the insecticidal effect, but the applicant has unexpectedly found that when the magnesium aluminum silicate is added too much, the wetting property is reduced, so the weight ratio of white carbon black and magnesium aluminum silicate needs to be controlled, thus obtaining the composition with better comprehensive wetting and suspension properties.
In addition, during transportation, the particles of the composition are often subjected to collision with each other or external pressure, while the saw-toothed structure of diatomaceous earth tends to form larger voids, which is detrimental to the stability of the composition particles, applicants have surprisingly found that when magnesium aluminum silicate and silica are used in combination, the hot press stability of the composition particles can be improved, probably because, when silica and magnesium aluminum silicate are added, because the large specific surface area and the hydroxyl of the white carbon black can be mutually adsorbed with the sawtooth-shaped diatomite and the chamaejasmine, the softer structure of the magnesium aluminum silicate and the structure of the organic cation are beneficial to the action of the sawtooth-shaped diatomite, thereby reducing the internal gaps of the prepared composition particles, not easily damaging the saw-tooth structure of the diatomite and increasing the stability of the composition particles, such as the composition in the dosage form of water dispersible granules. And the applicant found that when unmodified magnesium aluminum silicate is used, the adsorbability with diatomaceous earth is poor, and the stability under external force cannot be improved well.
In one embodiment, the raw materials for preparing the insecticidal composition of the present invention further comprise a dispersant.
Dispersing agent
In one embodiment, the dispersant of the present invention is selected from one or more of alkylphenol polyoxyethylene phosphate, fatty acid polyoxyethylene ether, alkyl naphthalene sulfonate, polycarboxylate, dodecyl sulfate, phosphate ester salt, lignosulfonate, alkylphenol polyoxyethylene ether, and alkylphenol formaldehyde resin polyoxyethylene ether.
Preferably, the dispersant accounts for 4-8 wt% of the total weight of the sawtooth-shaped diatomite and the chamaejasmine; further, the dispersant of the present invention accounts for 6 wt% of the total weight of the saw-tooth diatomaceous earth and the chamaejasmine.
More preferably, the dispersant of the present invention comprises lignosulfonate and polycarboxylate in a weight ratio of 1: (0.2 to 0.4); further, the weight ratio of the lignosulfonate to the polycarboxylate is 1: 0.3.
The lignosulfonate is a high molecular compound with the molecular weight of 200-30000. The low molecular lignin sulfonate is mainly prepared by calcium lignosulfonate, sodium lignosulfonate, magnesium lignosulfonate and the like, has different relative molecular masses and different structures, is generally straight chain and is associated together in solution; the high molecular lignosulfonate is mostly branched chain and is a non-uniform anionic polyelectrolyte with polydispersity. Is easily soluble in water, is not influenced by the change of Ph value, and is insoluble in ethanol, acetone and other common organic solvents. The aqueous solution has colloidal properties, and the viscosity of the solution increases with increasing concentration. The lignosulfonate has little effect on reducing the interfacial surface tension of liquid, cannot reduce the surface tension of water or form micelles, and has a dispersion effect mainly depending on adsorption-desorption of a matrix and generation of charges. Exhibit polymeric dielectric behavior in aqueous media. Can be used as a dispersant of pesticide, has better chelating ability on heavy metals, especially iron, copper and stannous ions, and is an effective chelating agent. The lignosulfonate is purchased or made by self, and in a preferred embodiment, the lignosulfonate of the invention is purchased from one or more of CA-SA (molecular weight <20000, degree of sulfonation 0.70), CA (molecular weight 20000, degree of sulfonation 0.70), NA (molecular weight 20000, degree of sulfonation 0.70) of bortresperse, NA (molecular weight 30000, degree of sulfonation 0.50) of Ultrazine, 3A (molecular weight 30000, degree of sulfonation 0.48), VSI D80 (molecular weight 7505, degree of sulfonation 0.82) of Meadwestvaco, VSI DW5 (molecular weight 11015, degree of sulfonation 0.81), VSI EDF350 (molecular weight 5709, degree of sulfonation 3.07) of Meadwestvaco; in a more preferred embodiment, the lignosulfonate of the present invention is available from CA or NA of Borresperse.
Further preferably, the molecular weight of the lignosulfonate of the invention is 20000-25000, and the sulfonation degree is 0.65-0.75; further, the molecular weight of the lignosulfonate of the invention is 20000, and the sulfonation degree is 0.70.
Molecular weight is the sum of the relative atomic masses of the atoms in the formula, as measured according to methods well known in the art.
The sulfonation degree reflects the content of sulfonic acid groups in the polymer, and has important influence on the structure and the performance of the sulfonate polymer. The degree of sulfonation according to the present invention is determined by methods known to those skilled in the art, such as precipitation, elemental analysis, ion exchange resin and conductivity titration, and acid-base titration.
The molecules of the polycarboxylate are of a comb-shaped or dendritic structure artificially formed by molecular design, and a plurality of branched chains (side chains) with certain length and rigidity are connected to the main chain of the molecule. The main chain also has sulfonate or other groups capable of charging the solid particles, and once the main chain is adsorbed on the surface of the solid particles, the branched chains form steric cross with branched chains on the surfaces of other particles, so that the particles are prevented from approaching each other, and the dispersion effect is achieved. This steric hindrance is not weakened by a prolonged period of time, and the dispersing action is more durable. The polycarboxylate of the present invention is purchased or made by oneself, and in a preferred embodiment, the polycarboxylate of the present invention is purchased from one or more of DC01, DC02, DC04, GY-JZS-B (rosin grafted polycarboxylate) of the widely-sourced agrichemical llc of beijing; in a more preferred embodiment, the polycarboxylate of the present invention is GY-JZS-B.
Still more preferably, the polycarboxylate of the present invention is a rosin-grafted polycarboxylate.
The applicant has unexpectedly found that when lignosulfonate and polycarboxylate, such as rosin-grafted polycarboxylate, are added to act together, wetting and suspending properties and stability under stress can be further improved, probably because strongly polar sulfonate in lignosulfonate is favorable for adsorbing on the surface of active ingredients such as saw-toothed diatomite, stellerin and the like, the branched chain structure of high molecular weight lignosulfonate forms a space bit group, and dispersibility of diatomite, stellerin and the like is improved, but because high molecular lignosulfonate cannot reduce surface tension, improvement on wetting effect is not significant, the applicant has found that by adding polycarboxylate, especially rosin-grafted polycarboxylate, interpenetrating network polymeric membrane can be formed with lignosulfonate, and because of macrocyclic conjugated structure and alternate branched chain structure of two dispersants, the thickness and the density of the polymer film are increased, the contact among effective components is reduced, the added rosin grafted carboxylate and macrocyclic and hydroxyl structures on the lignin dispersant are also beneficial to the action with fillers such as white carbon black, magnesium aluminum silicate and the like, and a more stable physical crosslinking system is formed, so that the suspension property is improved, and the polymer film of the interpenetrating network is also beneficial to effectively reducing the surface tension and promoting the wetting when acting on the surface of a plant.
In addition, when the composition is in the form of water dispersible granules, the rosin grafted carboxylate and the lignosulfonate can further reduce the gaps of the saw-tooth-shaped diatomite, and a protective film formed on the solid surfaces of the diatomite, the filler and the like has certain viscoelasticity, so that the damage to the structure of the saw-tooth-shaped diatomite and the structure of the water dispersible granules when the composition is stressed can be reduced. And the applicant finds that when the sulfonation degree is too high, the contact of the polycarboxylate, the diatomite and the chamaejasmine is not facilitated, an interpenetrating network structure is formed, the polycarboxylate is adsorbed on the surface of the lignosulfonate, the dispersibility effect of the lignosulfonate is reduced, and the suspension property and wettability improving effect is reduced.
In one embodiment, the formulation of the insecticidal composition of the present invention is selected from one or more of emulsifiable solution, wettable powder, water dispersible granule, suspending agent, aqueous emulsion, microemulsion, dispersible oil suspending agent, microcapsule suspending agent and soluble powder.
In a second aspect, the present invention provides a wettable powder, which is prepared from the insecticidal composition comprising the above-mentioned saw-toothed diatomite and chamaejasmine.
The preparation method of the wettable powder is a well-known preparation method of the invention. For reference, it can be mentioned that, in a more preferred embodiment, the method for preparing the wettable powder of the present invention comprises the following steps: and mixing and crushing the chamaejasmine, the filler and the dispersing agent, and adding the sawtooth-shaped diatomite for mixing to obtain the wettable powder.
The invention provides a water dispersible granule, which is prepared from the raw materials of the water dispersible granule, wherein the raw materials comprise the insecticidal composition containing the sawtooth-shaped diatomite and the chamaejasmine.
The preparation raw materials of the water dispersible granule can also comprise conventional auxiliary materials or carriers in pesticide preparations, including but not limited to any one or more of thickening agent, antifreezing agent, stabilizing agent, preservative, disintegrating agent, binder or defoaming agent; for example, the thickener may be selected from any one or more of xanthan gum, carboxymethyl cellulose, sodium polyacrylate, magnesium aluminium silicate, gaseous silica or attapulgite; the antifreezing agent can be selected from any one or a mixture of more than one of ethylene glycol, propylene glycol, glycerol-diethyl ether diglycol or methyl propylene diglycol according to any proportion; the stabilizer can be selected from any one or more of 2, 6-di-tert-butyl-4-methylphenol (BHT), citric acid or Butyl Hydroxy Anisole (BHA); the preservative can be sodium benzoate; the disintegrating agent can be selected from one or more of urea, ammonium sulfate, sodium carboxymethyl starch, sodium carbonate, bentonite, montmorillonite or sodium alginate; the binder can be selected from one or more of gelatin, Arabic gum, polyethylene glycol, anhydrous glucose, polyvinyl alcohol, xanthan gum, sodium tripolyphosphate, phenolic resin or polyvinylpyrrolidone; the defoaming agent can be defoaming agent such as organosilicon.
In a well-known embodiment, the raw materials for preparing the water dispersible granule also comprise a disintegrating agent and a binder; furthermore, the disintegrating agent accounts for 0.1-3 wt% of the total weight of the saw-tooth-shaped diatomite and the chamaejasmine, and the binder accounts for 0.1-3 wt% of the total weight of the saw-tooth-shaped diatomite and the chamaejasmine.
The preparation method of the water dispersible granule is a method well known in the field, and for reference, the preparation method of the water dispersible granule comprises the following steps: mixing and crushing the preparation raw materials of the water dispersible granules except the saw-tooth-shaped diatomite, adding the saw-tooth-shaped diatomite, kneading, granulating and drying to obtain the water dispersible granule.
In a fourth aspect, the present invention provides the use of an insecticidal composition comprising diatomaceous earth zigzag and chamaejasmine as described above for controlling plant pests.
The applicant finds that the effect of preventing plant pests can be obviously improved by compounding the sawtooth-shaped diatomite and the chamaejasmine, and the compound pesticide has a high-efficiency insecticidal effect on Frankliniella occidentalis, whiteflies, liriomyza sativae, prodenia litura, tea lesser leafhoppers, tea yellowhorn thistles, tea geometrids, green plant bugs, grape aphids and the like.
Examples
The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.
Example 1
The embodiment provides an insecticidal composition containing sawtooth-shaped diatomite and chamaejasmine, and the preparation raw materials of the insecticidal composition comprise the sawtooth-shaped diatomite, a filler and a dispersing agent, wherein the filler accounts for 8 wt% of the total weight of the sawtooth-shaped diatomite, the filler comprises white carbon black and magnesium aluminum silicate, and the weight ratio of the white carbon black to the magnesium aluminum silicate is 1: 0.6, wherein the dispersant accounts for 6 wt% of the total weight of the sawtooth-shaped diatomite, and comprises lignosulfonate and polycarboxylate, wherein the weight ratio is 1: 0.3.
the diatomaceous earth in saw-tooth form was purchased from Celite610 of the garnets, france.
The white carbon black is HDK T30 of watt gram.
The magnesium aluminum silicate is R.T.Vanderbilt magnesium aluminum silicate PRO.
The lignosulfonate is the NA of Borresperse.
The polycarboxylate is rosin grafted polycarboxylate, and is GY-JZS-B of Beijing broad-source agricultural chemistry Limited liability company.
This example also provides a wettable powder prepared from the insecticidal composition comprising the serrated diatomaceous earth and chamaejasmine as described above.
The embodiment also provides a preparation method of the wettable powder, which comprises the following steps: and mixing and crushing the filler and the dispersing agent, and adding the sawtooth-shaped diatomite for mixing to obtain the wettable powder.
The embodiment also provides a water dispersible granule, and the preparation raw materials of the water dispersible granule comprise the sawtooth-shaped diatomite and chamaejasmine insecticidal composition, a disintegrating agent and a binder; the disintegrating agent accounts for 2 wt% of the total weight of the sawtooth-shaped diatomite, and the binder accounts for 2 wt% of the total weight of the sawtooth-shaped diatomite; the disintegrating agent is ammonium sulfate, and the binder is Arabic gum, which is purchased from Hebei Pengyu biotechnology Limited.
The embodiment also provides a preparation method of the water dispersible granule, which comprises the following steps: mixing and crushing the preparation raw materials of the water dispersible granule except the saw-tooth-shaped diatomite, adding the saw-tooth-shaped diatomite, kneading, granulating and drying to obtain the water dispersible granule.
Example 2
The embodiment provides an insecticidal composition containing sawtooth-shaped diatomite and chamaejasmine, and the preparation raw materials of the insecticidal composition comprise chamaejasmine, a filler and a dispersing agent, wherein the filler accounts for 8 wt% of the total weight of the chamaejasmine, the filler comprises white carbon black and magnesium aluminum silicate, and the weight ratio is 1: 0.6, the dispersant accounts for 6 wt% of the total weight of the chamaejasmine, and comprises lignosulfonate and polycarboxylate, wherein the weight ratio is 1: 0.3.
the chamaejasmine was purchased from Shanghai Qincheng Biotech, Inc.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, wherein the preparation raw materials of the wettable powder comprise the saw-tooth-shaped diatomite and the chamaejasmine insecticidal composition.
The embodiment also provides a preparation method of the wettable powder, which comprises the following steps: and mixing and crushing the chamaejasmine, the filling material and the dispersing agent to obtain the wettable powder.
The embodiment also provides a water dispersible granule, and the preparation raw materials of the water dispersible granule comprise the sawtooth-shaped diatomite and chamaejasmine insecticidal composition, a disintegrating agent and a binder; the disintegrant accounts for 2 wt% of the total weight of the chamaejasmine, and the binder accounts for 2 wt% of the total weight of the chamaejasmine; the disintegrating agent is ammonium sulfate, and the binder is Arabic gum.
The embodiment also provides a preparation method of the water dispersible granule, which comprises the following steps: the preparation raw materials of the water dispersible granule are mixed, crushed, kneaded, granulated and dried to obtain the water dispersible granule.
Example 3
The embodiment provides an insecticidal composition containing saw-toothed diatomite and chamaejasmine, which is prepared from the following raw materials in parts by weight of 200: 1, the filler accounts for 8 wt% of the total weight of the sawtooth-shaped diatomite and the chamaejasmine, the filler comprises white carbon black and magnesium aluminum silicate, and the weight ratio is 1: 0.6, the dispersant accounts for 6 wt% of the total weight of the saw-tooth diatomite and the chamaejasmine, and the dispersant comprises lignosulfonate and polycarboxylate, wherein the weight ratio is 1: 0.3.
this example also provides a wettable powder prepared from the insecticidal composition comprising the serrated diatomaceous earth and chamaejasmine as described above.
The embodiment also provides a preparation method of the wettable powder, which comprises the following steps: and mixing and crushing the chamaejasmine, the filler and the dispersing agent, and adding the sawtooth-shaped diatomite for mixing to obtain the wettable powder.
The embodiment also provides a water dispersible granule, and the preparation raw materials of the water dispersible granule comprise the sawtooth-shaped diatomite and chamaejasmine insecticidal composition, a disintegrating agent and a binder; the disintegrating agent accounts for 2 wt% of the total weight of the saw-tooth-shaped diatomite and the chamaejasmine, and the binder accounts for 2 wt% of the total weight of the saw-tooth-shaped diatomite and the chamaejasmine; the disintegrating agent is ammonium sulfate, and the binder is Arabic gum.
The embodiment also provides a preparation method of the water dispersible granule, which comprises the following steps: mixing and crushing the preparation raw materials of the water dispersible granule except the saw-tooth-shaped diatomite, adding the saw-tooth-shaped diatomite, kneading, granulating and drying to obtain the water dispersible granule.
Example 4
The embodiment of the insecticidal composition containing the saw-tooth-shaped diatomite and the chamaejasmine is the same as that in example 3, except that the weight ratio of the saw-tooth-shaped diatomite to the chamaejasmine is 100: 1.
the embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 5
The embodiment of the insecticidal composition containing the saw-tooth-shaped diatomite and the chamaejasmine is the same as that in example 3, except that the weight ratio of the saw-tooth-shaped diatomite to the chamaejasmine is 1: 1.
the embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 6
The embodiment of the insecticidal composition containing the saw-tooth-shaped diatomite and the chamaejasmine is the same as that in example 3, except that the weight ratio of the saw-tooth-shaped diatomite to the chamaejasmine is 1: 20.
the embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 7
The embodiment of the insecticidal composition containing the saw-tooth-shaped diatomite and the chamaejasmine is the same as that in example 3, except that the weight ratio of the saw-tooth-shaped diatomite to the chamaejasmine is 1: 40.
the embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 8
This example provides an insecticidal composition comprising crenellated diatomaceous earth and chamaejasmine, which is similar to example 4, except that the filler does not comprise magnesium aluminum silicate.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 9
The embodiment of the insecticidal composition containing the saw-toothed diatomite and the chamaejasmine is the same as that in example 4, except that the white carbon black is Wake HDK T40.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 10
The embodiment of the insecticidal composition containing the saw-toothed diatomite and the chamaejasmine is the same as that in example 4, except that the white carbon black is the Wake HDK V15.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 11
This example provides an insecticidal composition comprising crenellated diatomaceous earth and chamaejasmine, which is similar to example 4, except that the magnesium aluminum silicate is r.t. vanderbilt's magnesium aluminum silicate HS.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 12
The embodiment of the insecticidal composition containing the saw-toothed diatomite and the chamaejasmine is the same as that in example 4, except that the weight ratio of the white carbon black to the magnesium aluminum silicate is 1: 1.5.
the embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 13
This example provides an insecticidal composition comprising crenellated diatomaceous earth and chamaejasmine, which is similar to example 4, except that the dispersant does not include a polycarboxylate.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 14
This example provides an insecticidal composition comprising crenellated diatomaceous earth and chamaejasmine, which is similar to example 4, except that the sodium lignosulfonate is VSI DW5 from Meadwestvaco.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 15
This example provides an insecticidal composition comprising diatomaceous earth in saw tooth form and chamaejasmine, which is similar to example 4, except that the sodium lignosulfonate is VSI EDF350 from Meadwestvaco.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 16
This example provides an insecticidal composition comprising crenellated diatomaceous earth and chamaejasmine, which is similar to example 4, except that the lignosulfonate is NA of Ultrazine.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Example 17
This example provides an insecticidal composition comprising crenellated diatomaceous earth and chamaejasmine, which is similar to example 4, except that the polycarboxylate is DC04 from the company mikyo, guangyo chemical, llc.
The embodiment also provides a wettable powder and a preparation method of the wettable powder, and the specific implementation mode is the same as that of the embodiment 3.
The embodiment also provides a water dispersible granule and a preparation method thereof, and the specific implementation mode is the same as that of the embodiment 3.
Evaluation of Performance
1. And (3) combined virulence determination: the insecticidal compositions provided in the examples and comparative examples were subjected to combined virulence determination on frankliniella occidentalis, powdery mildew, liriomyza sativae, prodenia litura, tea lesser leafhopper, tea thrips, tea geometrid, green plant bug and grape aphid, and the specific test methods and results are as follows:
(1) combined toxicity determination of crenellated diatomite and chamaejasmine compounded to frankliniella occidentalis
According to the standard method NY/T1154.14-2008 of biological measurement, a leaf-soaking dish method is adopted. Beating the leaves into leaf disks by using a hole puncher with the diameter of 18mm, soaking the leaf disks in a test reagent solution for 10 seconds, putting the leaf disks into a culture dish (with the diameter of 90mm) padded with moisturizing filter paper, putting 5 leaf disks into each dish, naturally drying, then selecting the Frankliniella occidentalis adults into the culture dish, sealing the mouth of the dish by using a preservative film, pricking small holes on the film by using a needle for ventilation, repeating the treatment for 4 times, finishing the treatment, and placing the treated leaves into an observation room for culture.
And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that the test insects are dead according to the condition that the test insects are slightly contacted with the pointed writing brush and do not react.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 is an antagonistic effect, and between 80 and 120 is an additive effect. The co-toxicity coefficient is calculated according to the formula
The Theoretical Toxicity Index (TTI) of the mixture is the toxicity index of the agent A x the percent (%) of the agent A + the toxicity index of the agent B x the percent (%) of the agent B
The results are shown in Table 1.
TABLE 1 toxicity test results of saw-toothed diatomaceous earth and chamaejasmine
(2) Combined toxicity determination method for trialeurodes vaporariorum by compounding sawtooth-shaped diatomite and stellera chamaejasme
Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. After dissolving agar (1%) by heating, 20mL to 200mL of the solution was taken out of a beaker and condensed at room temperature for use. Soaking the leaves in a test solution for 10 seconds, taking out, naturally drying, sticking the leaves on the surface of the solidified agar solution, wrapping petioles with wet cotton balls, putting 3-4 leaves in each beaker, inoculating adult trialeurodes vaporariorum, covering 15 heads of each beaker with a preservative film, pricking small holes on the film with needles for ventilation, repeating the treatment for 4 times, and placing the beaker in an observation room for cultivation after the treatment.
And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the test insects are lightly contacted with tweezers.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 2.
TABLE 2 toxicity test results of mixing of saw-toothed diatomaceous earth and chamaejasmine against Bemisia alba
(3) Combined toxicity determination of sawtooth-shaped diatomite and stellera chamaejasme compound on liriomyza sativae
Referring to the method in NY/T1154.13-2008 standard, spraying method is adopted. Transplanting potted cowpea pest-free seedlings into a pest cage, inoculating a certain amount of mated adult Nanmei liriomyza, taking out bean seedlings with Nanmei liriomyza eggs after 6h, placing the bean seedlings in another pest cage, continuously breeding to 2-instar larvae (ensuring that each plate contains no less than 80 larvae at 2 instar), then carrying out spraying treatment, naturally drying, and transferring to an artificial climate box for continuous breeding. Each treatment was repeated 4 times and a blank control was run.
And (3) cutting bean seedlings from roots at the later stage of 3-year-old larvae, moisturizing the tail ends by using soaked cotton balls, putting the bean seedlings into plastic basins in an artificial climate box, pupating the bean seedlings, and counting the quantity of pupae in each dish.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 3.
TABLE 3 toxicity test results of the mixture of saw-toothed diatomaceous earth and chamaejasmine to Liriomyza sativae
(4) Combined toxicity determination of zigzag diatomite and chamaejasmine compounded paired spodoptera litura
Referring to the method in NY/T1154.14-2008 standard, the leaf-dipping dish method is adopted. The cabbage leaves are punched into leaf disks by a hole puncher with the diameter of 18mm, the leaf disks are soaked in a test reagent solution for 10 seconds and then placed into culture dishes (with the diameter of 90mm) filled with moisturizing filter paper, 6 leaf disks are placed into each dish, after the leaf disks are naturally dried, 3-year-old larvae of the spodoptera litura hungry for 4 hours are selected to be placed into the culture dishes, each dish is not lower than 10, the process is repeated for 4 times, the dishes are covered with a dish cover, and the culture is placed in an observation room.
And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the test insects are lightly contacted with tweezers.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 4.
TABLE 4 toxicity test results of Twill when saw-toothed diatomaceous earth and chamaejasmine are mixed
(5) Combined toxicity determination of sawtooth-shaped diatomite and chamaejasmine compounded tea lesser leafhopper
Referring to the method in NY/T1154.11-2008 standard, the dipping method is adopted. Selecting 3 fresh tea tree sprouts, soaking in a test solution for 30 seconds, taking out, naturally drying, wrapping the base parts of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) with the concentration repeated for 4 times. Then, 3-year-old nymphs of tea lesser leafhoppers subjected to ether mild anesthesia treatment are inoculated into a brush pen, 15 +/-2 heads of each tube are used, and the mouth of each tube is sealed by white gauze. After the treatment, the sample is placed in an observation chamber.
And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the test insects are lightly contacted with tweezers.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 5.
TABLE 5 toxicity test results of tea leafhopper by mixing of saw-toothed diatomaceous earth and chamaejasmine
(6) Combined toxicity determination of tea yellow thrips by compounding sawtooth-shaped diatomite and chamaejasmine
According to the standard method NY/T1154.14-2008 of biological measurement, a leaf dipping method is adopted. Selecting 3 fresh tea tree sprout shoots with a certain amount of tea thrips (no less than 20) and soaking in the test liquid medicine for 10 seconds, taking out and airing, wrapping the base parts of the sprout shoots with wet absorbent cotton to preserve moisture, sealing the absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) for 4 times of repetition per concentration. The mouth of the test tube is sealed by a preservative film, and the small holes are punched for ventilation. After the treatment, the sample is placed in an observation chamber.
And after 72h, examining the death condition of the test insects under a dissecting mirror, and recording, wherein the death judgment standard of the test insects is that the test insects die as the result of no response when the test insects are lightly contacted by a pointed hair pen.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 6.
TABLE 6 toxicity test results of mixing of saw-toothed diatomaceous earth and chamaejasmine to tea leaf yellow thrips
(7) Combined toxicity determination of sawtooth-shaped diatomite and chamaejasmine compounded tea geometrid
Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. Selecting 3 fresh tea tree sprouts, soaking in a test solution for 30 seconds, taking out, naturally drying, wrapping the bases of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a can bottle, wherein the concentration is repeated for 4 times. Then, live and healthy 2-year nymphs of tea geometrid with consistent size are inoculated by a brush pen, 15 +/-2 heads of each pot are sealed by white gauze. After the treatment, the sample is placed in an observation chamber.
And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the test insects are lightly contacted with tweezers.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 7.
TABLE 7 toxicity test results of mixing of saw-toothed diatomaceous earth and chamaejasmine to tea geometrid
(8) Combined toxicity determination of crenellated diatomite and chamaejasmine compounded apolygus lucorum
Referring to the method in NY/T1154.11-2008 standard, a leaf spray method was used. The method comprises the steps of disinfecting, cleaning and airing fresh kidney beans by adopting an immersion method, cutting the kidney beans into 2-3 cm segments, immersing the kidney beans in liquid medicines with different concentrations for 30 seconds, airing the kidney beans under natural conditions, and then putting the kidney beans into transparent plastic boxes (6 cm in diameter and 7cm in height) with smooth air, wherein each box has two sections. Healthy and consistent 3-year-old nymphs of lygus lucorum were inoculated, each box was repeated for 4 times, and a blank control was set. And putting the plastic box into a light incubator for culturing.
And after 48h, the death condition of the test insects in each box is checked, the bodies of the lygus lucorum are lightly touched by the tip of the writing brush, and the death judgment standard of the test insects is that the death is counted by that no response is caused when the tweezers lightly touch the test insects.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 8.
Table 8 toxicity test results of Apolygus lucorum mixed by diatomite in shape and chamaejasmine
(9) Combined toxicity determination of crenellated diatomite and stellera chamaejasme compound grape aphid
The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Cutting leaves with a certain amount of grape aphids, soaking in the liquid medicine to be tested for 10 seconds, taking out, absorbing the liquid medicine around the insect body with absorbent filter paper, placing in a culture dish padded with moisturizing filter paper, moisturizing with a wet cotton ball, and covering the culture dish with wet gauze. Each 30 aphids were treated, 4 replicates and a blank treatment without drug (including solvent and emulsifier used) was used. After the treatment, the cells were transferred to an observation room for cultivation.
And (3) detecting the death condition of the test insects after 48h, and recording, wherein the judgment standard of the death of the test insects is as follows: the aphids were lightly touched with a writing brush, and the body was judged dead.
The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. The specific calculation method is shown in (1), and the results are shown in Table 9.
TABLE 9 toxicity test results of blending of saw-toothed diatomaceous earth and chamaejasmine on grape aphid
2. Suspension percentage: the suspension rate of the wettable powder provided by the embodiment is determined by the GB/T14825-2006 method, and the wettable powder is graded according to the suspension rate, wherein the suspension rate is greater than or equal to 85% in grade 1, greater than or equal to 80% in grade 2, less than 85% in grade 3, greater than or equal to 70% in grade 3, less than 80% in grade 4, greater than or equal to 60% in grade 4, less than 70% in grade 5, and the results are shown in Table 10.
3. Wettability: the wetting time of the wettable powder provided by the embodiment is measured according to the GB/T5451-2001 method, and is rated as 1-5 grade according to the wetting time, wherein the wetting time of 5 grade is more than 120s, the wetting time of 4 grade is more than 100s and less than or equal to 120s, and the wetting time of 3 grade is more than 80s and less than or equal to 100 s; the wetting times for the 2-stage were greater than 60s and less than or equal to 80s, and the wetting times for the 1-stage were less than or equal to 60s, the results are shown in Table 10.
4. And (3) hot-pressing stability: the water dispersible granules provided in the example were extruded under a pressure of 60g/cm3, and then placed in an oven at 50 ℃, and the state of the water dispersible granules after 24 hours was observed and rated, wherein grade 1 was no caking and caking, grade 2 was slightly caking and no caking, grade 3 was an age increase and no caking, grade 4 was significant caking and slightly caking, grade 5 was significant caking, and the results are shown in table 10.
Table 10 characterization test of properties
| Examples | Suspension rate | Wettability | Thermal stabilityCharacterization of nature |
| 4 | Level 1 | Level 1 | Level 1 |
| 8 | 4 stage | Stage 2 | 4 stage |
| 9 | Grade 3 | Level 1 | |
| 10 | Stage 2 | Grade 3 | |
| 11 | Stage 2 | Stage 2 | Grade 3 |
| 12 | Stage 2 | Grade 3 | Grade 3 |
| 13 | Stage 2 | 4 stage | |
| 14 | Grade 3 | Stage 2 | |
| 15 | 4 stage | Stage 2 | Grade 3 |
| 16 | Stage 2 | Grade 3 | Stage 2 |
| 17 | Level 1 | Grade 3 | Grade 3 |
The test results in tables 1-10 show that the insecticidal composition provided by the invention is prepared by compounding the sawtooth-shaped diatomite and the chamaejasmine in a weight ratio of 200: 1-1: 40 can have synergistic insecticidal effect, and has good control effect on various insects such as Frankliniella occidentalis, whitefly, leaf miner, prodenia litura, tea lesser leafhopper, tea yellow thrips, tea geometrid, green plant bug, grape aphid, etc.; in addition, the applicant finds that by selecting proper fillers and dispersants, the damage to the zigzag structure of the diatomite in the preparation and transportation process can be reduced while good wetting and suspension properties are ensured, and the storage stability is improved.
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Where numerical ranges are used in the claims, also included are sub-ranges within them, and variations in these ranges are also to be construed as being covered by the appended claims where possible.
Claims (10)
1. The insecticidal composition containing the saw-toothed diatomite and the chamaejasmine is characterized in that the preparation raw materials of the insecticidal composition comprise the saw-toothed diatomite and the chamaejasmine in a weight ratio of 200: 1-1: 40.
2. the insecticidal composition containing serrated diatomaceous earth and chamaejasmine according to claim 1, wherein the raw materials for preparing the insecticidal composition further comprise a filler, and the filler is one or more selected from kaolin, white carbon black, magnesium aluminum silicate, anhydrous sodium sulfate, glucose, pottery clay, sericite, fructose and soluble starch.
3. The insecticidal composition containing saw-toothed diatomite and chamaejasmine according to claim 2, wherein the filler accounts for 5-10 wt% of the total weight of the saw-toothed diatomite and the chamaejasmine.
4. The insecticidal composition containing saw-toothed diatomite and chamaejasmine according to claim 2, wherein the filler comprises white carbon black, and the white carbon black has a specific surface area of 300-500 m2/g。
5. The insecticidal composition comprising diatomaceous earth and chamaejasmine as claimed in claim 4, wherein the filler further comprises magnesium aluminum silicate, and the white carbon and magnesium aluminum silicate are present in a weight ratio of 1: (0.5 to 1.5).
6. The insecticidal composition containing the serrated diatomaceous earth and the chamaejasmine according to any one of claims 1 to 5, wherein the preparation raw materials of the insecticidal composition further comprise a dispersing agent, and the dispersing agent is one or more selected from alkylphenol polyoxyethylene phosphate, fatty acid polyoxyethylene ether, alkyl naphthalene sulfonate, polycarboxylate, dodecyl sulfate, phosphate ester salt, lignosulfonate, alkylphenol polyoxyethylene ether and alkylphenol formaldehyde resin polyoxyethylene ether.
7. The insecticidal composition containing saw-toothed diatomite and chamaejasmine according to claim 6, wherein the dispersant accounts for 4-8 wt% of the total weight of the saw-toothed diatomite and the chamaejasmine.
8. A wettable powder, which is characterized in that the preparation raw materials of the wettable powder comprise the insecticidal composition containing the serrated diatomite and the chamaejasmine as claimed in any one of claims 1 to 7.
9. A water dispersible granule, which is characterized in that the preparation raw material of the water dispersible granule comprises the insecticidal composition containing the serrated diatomite and the chamaejasmine as claimed in any one of claims 1-7.
10. Use of the insecticidal composition according to any one of claims 1 to 9, containing the saw-toothed diatomaceous earth and the chamaejasmine for controlling plant pests.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010110401.8A CN111296423A (en) | 2020-02-24 | 2020-02-24 | Insecticidal composition containing saw-toothed diatomite and chamaejasmine and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010110401.8A CN111296423A (en) | 2020-02-24 | 2020-02-24 | Insecticidal composition containing saw-toothed diatomite and chamaejasmine and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111296423A true CN111296423A (en) | 2020-06-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010110401.8A Withdrawn CN111296423A (en) | 2020-02-24 | 2020-02-24 | Insecticidal composition containing saw-toothed diatomite and chamaejasmine and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111296423A (en) |
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2020
- 2020-02-24 CN CN202010110401.8A patent/CN111296423A/en not_active Withdrawn
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Application publication date: 20200619 |