CN111436423A

CN111436423A - Novel insecticidal pesticide composition

Info

Publication number: CN111436423A
Application number: CN202010112260.3A
Authority: CN
Inventors: 刘永山
Original assignee: Guangxi Yicai Agricultural Development Co ltd
Current assignee: Guangxi Yicai Agricultural Development Co ltd
Priority date: 2020-02-24
Filing date: 2020-02-24
Publication date: 2020-07-24

Abstract

The invention relates to the technical field of pesticides, in particular to a novel insecticidal pesticide composition. A novel insecticidal pesticide composition is characterized by comprising diatomite with a specific structure and a raw pesticide substance as effective components, wherein the mass ratio of the diatomite with the specific structure to the raw pesticide substance is (1: 60) - (300: 1); the technical material is selected from any one of carbaryl, isoprocarb, metolcarb, pirimicarb and methomyl. The invention provides a novel insecticidal pesticide composition, which is prepared by mixing diatomite and a raw material medicament in a special structure, can achieve the effect of preventing and controlling pests under the using amount of a small amount of raw material medicament, and simultaneously, the developed novel diatomite and the developed complex system of the raw material medicament have higher co-toxicity coefficient, can reduce the selective pressure generated by singly and continuously using the medicament to prevent and control the pests, slow down the development of the drug resistance of the pests, reduce the using amount of the pesticides, reduce the environmental pollution, increase the grain safety, and accord with the national policy of reducing the using amount of fertilizers and pesticides.

Description

Novel insecticidal pesticide composition

Technical Field

The invention relates to the technical field of pesticides, in particular to a novel insecticidal pesticide composition.

Background

Carbaryl is a broad-spectrum carbamate pesticide with high efficiency, low toxicity, low residue and long residual effect. Has strong contact poisoning effect on pests, stomach toxicity and slight systemic effect. Is used for preventing and treating cotton bollworm, leaf roller, cotton aphid, desmodiasis, thrips, rice leafhopper, rice leaf roller, rice bract, rice thrips and fruit tree pests. Isoprocarb, o-isopropylphenyl methylcarbamate, acts on insects by inhibiting acetylcholinesterase, causing the insects to paralyze to die. Has special effect on rice planthopper and leafhopper pests. The pesticide has strong knockdown force and quick pesticide effect, can treat thrips and leeches, and is safe to natural enemies of rice planthoppers and spiders. The metolcarb belongs to a quick-acting substituted benzene carbamate pesticide with certain selectivity, has contact poisoning and fumigation effects on pests, also has a certain systemic effect, and has an action mechanism of inhibiting acetylcholinesterase so as to cause paralysis and death of insects. Pirimicarb (Pirimicarb) is a carbamate selective aphicide developed by american phylum of Bulkinomen, has contact, fumigation and systemic effects, has permeability on leaf tissues, and can effectively control aphids resistant to organic phosphorus. The methomyl is a systemic carbamate pesticide, acts on cholinesterase of pests, destroys nervous systems of the pests and causes the pests to die, has the effects of contact poisoning and stomach poisoning, fumigation, strong permeability and certain ovicidal effect, and has better control effect on the pests with resistance to organophosphorus and pyrethroid.

Diatomaceous earth is a white soft sedimentary stone that can be easily ground into powder. Low density, porous, rough feeling, and is a fossil of diatom. The common diatomite has insecticidal action, damages the outer wax-like layer of the exoskeleton of insects by physical adsorption, leads the insects to die by dehydration, and can be used as an insecticide, but the insecticidal effect is not obvious, and the common diatomite is often only useful for certain specific insects, so the common diatomite is only used as an adsorbent in pesticides.

However, in the current market, a single medicament is continuously used for preventing and treating pests for a long time, so that the pests are easy to generate drug resistance, and meanwhile, pesticide is applied in a large amount due to the generation of the drug resistance, so that the residual pesticide amount in the environment is increased, hidden dangers are brought to the ecological environment and the grain safety, and the national policy of reducing the using amount of fertilizer and pesticide is not met.

Disclosure of Invention

In order to solve the technical problems, the invention provides a novel insecticidal pesticide composition, which comprises diatomite with a specific structure and a raw pesticide substance as effective components, wherein the mass ratio of the diatomite with the specific structure to the raw pesticide substance is (1: 60) - (300: 1); the technical material is selected from any one of carbaryl, isoprocarb, metolcarb, pirimicarb and methomyl.

As a preferable technical scheme, the diatomite with the specific structure has a sawtooth-shaped honeycomb structure.

As a preferable technical scheme, the mass ratio of the diatomite with the specific structure to the carbaryl is (1: 60) - (300: 1).

As a preferable technical scheme, the mass ratio of the diatomite with the specific structure to the isoprocarb is (1: 60) - (300: 1).

In a preferable embodiment, the mass ratio of the diatomaceous earth with the specific structure to the metolcarb is (1: 60) - (200: 1).

As a preferable technical scheme, the mass ratio of the diatomite with the specific structure to the pirimicarb is (1: 60) - (200: 1).

As a preferable technical scheme, the mass ratio of the diatomite with the specific structure to the methomyl is (1: 60) - (200: 1).

As a preferable technical scheme, the composition is any one preparation formulation prepared by taking diatomite with a specific structure and original medicine substances as active ingredients and compounding with pesticide auxiliary agents.

As a preferable technical scheme, the pesticide auxiliary agent is selected from one or more of an adsorbent, a dispersing agent, a wetting agent, a tackifier and a synergist.

As a preferable technical scheme, the preparation formulation is any one of missible oil, suspending agent, wettable powder, granules, aqueous solution, poison bait, mother solution and mother powder.

As a preferable technical scheme, the content of the effective components in the composition is 0.001-99.999 wt%.

As a preferable technical scheme, the content of the effective components in the composition is 30-70 wt%.

In a second aspect, the present invention provides a method for preparing a wettable powder containing the pesticidal composition as described above, comprising the steps of: weighing effective components and pesticide adjuvant, and performing high-speed universal pulverization; and adding diatomite with a specific structure, shaking, transferring into a flask, stirring and mixing for 30min by using a stirrer, and fully and uniformly mixing.

In a third aspect, the present invention provides a method for preparing a wettable powder containing the pesticide composition as described above, comprising the steps of: weighing effective components and pesticide adjuvant, and performing high-speed universal pulverization; and adding diatomite with a specific structure into the flask, shaking, transferring the mixture into the flask, stirring and mixing the mixture for 30min by using a stirrer, fully and uniformly mixing the mixture, and then granulating the mixture.

Has the advantages that: the invention provides a novel insecticidal pesticide composition, which is prepared by mixing diatomite and a raw material medicament in a special structure, can achieve the effect of preventing and controlling pests under the using amount of a small amount of raw material medicament, and simultaneously, the developed novel diatomite and the developed complex system of the raw material medicament have higher co-toxicity coefficient, can reduce the selective pressure generated by singly and continuously using the medicament to prevent and control the pests, slow down the development of the drug resistance of the pests, reduce the using amount of the pesticides, reduce the environmental pollution, increase the grain safety, and accord with the national policy of reducing the using amount of fertilizers and pesticides.

Drawings

Fig. 1 is an optical microscope of the diatomite having a specific structure of the saw-toothed honeycomb structure according to the present invention.

FIG. 2 is an electron micrograph of a specific structure diatomaceous earth having a saw-toothed honeycomb structure according to the present invention.

Detailed Description

Unless otherwise indicated, implied from the context, or customary in the art, all parts and percentages herein are by weight and the testing and characterization methods used are synchronized with the filing date of the present application. To the extent that a definition of a particular term disclosed in the prior art is inconsistent with any definitions provided herein, the definition of the term provided herein controls.

The technical features of the technical solutions provided by the present invention are further clearly and completely described below with reference to the specific embodiments, and the scope of protection is not limited thereto.

The words "preferred", "preferably", "more preferred", and the like, in the present invention, refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention. The sources of components not mentioned in the present invention are all commercially available.

In the present invention, the term "composition" means that the components of the composition are packaged in combination or separately and used together when used. In the invention, the diatomite with the specific structure and the original drug substance are mixed and packaged together, and are used together when in use; also comprises the steps of separately packaging the diatomite with a specific structure and the original medicine substances, and mixing the diatomite and the original medicine substances together according to a proportion when in use.

(diatomaceous earth of specific structure)

The diatomite with the specific structure is diatomite with a specific crystal structure, and generally, the diatomite contains main components of silicon dioxide and also contains silicon dioxideSmall amount of Al₂O₃、Fe₂O₃CaO, MgO, etc., and organic matter. The diatomite with a specific structure is diatomite with a specific sawtooth-shaped honeycomb structure silicon dioxide content after being purified.

In a preferred embodiment, the specially structured diatomaceous earth has a saw-tooth honeycomb structure.

In a preferred embodiment, the silica with a specific saw-tooth-shaped honeycomb structure can be diatomite with a specific structure under the trade name of Celite610 from the company of viry stones france. As shown in FIGS. 1 and 2, under an optical microscope and an electron microscope, Celite610 can be seen to have a clear saw-toothed honeycomb structure.

The optical micrograph was measured by an O L YMPUS C × 31 biomicroscope (16 × 40 times, Olympus, Japan) and the micrograph was measured by a conventional method.

On one hand, the diatomite with the specific structure has a larger specific surface area due to the saw-tooth-shaped honeycomb structure, and is favorable for being better adsorbed on a wax layer on the body surface of the insect, so that the insect body is quickly dehydrated. On the other hand, due to the zigzag honeycomb structure, the composition is beneficial to causing damage to a wax layer on the surface of the insect body, tearing the surface of the insect body, rapidly permeating into the insect body and causing fatal damage to the insect, thereby improving the lethality of the composition.

And the composition can act with the original medicine under the participation of the pesticide auxiliary agent through the synergistic effect of the composition and the original medicine substance, the original medicine substance can rapidly permeate under the driving of the diatomite, the control efficiency of the composition on pests is obviously improved, and the composition has high quick-acting performance and lethality. In addition, aiming at the acid-base instability of the carbaryl and the pirimicarb, the special structure of the diatomite can adsorb and protect a great amount of raw pesticide to stably exist under the conditions, the diatomite with the sawtooth structure and the raw pesticide are not easy to be folded into blocks after being compounded, the lasting period of the isoprocarb, the metolcarb and the methomyl is greatly prolonged, and the insecticidal effect is ensured.

(Carbaryl)

The carbaryl (carbaryl) is a broad-spectrum carbamate pesticide with high efficiency, low toxicity, low residue and long residual effect. Has strong contact poisoning effect on pests, stomach toxicity and slight systemic effect. Is used for preventing and treating cotton bollworm, leaf roller, cotton aphid, desmodiasis, thrips, rice leafhopper, rice leaf roller, rice bract, rice thrips and fruit tree pests.

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the carbaryl is (1: 60) - (300: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the carbaryl is (1: 40) - (200: 1).

In a preferred embodiment, the mass ratio of the diatomaceous earth with a specific structure to carbaryl is (1: 40) - (100: 1).

(isoprocarb)

The isoprocarb (Isoprocorb) and the o-isopropylphenyl methyl carbamate have the effect of inhibiting acetylcholinesterase on insects so that the insects are paralyzed to die. Has special effect on rice planthopper and leafhopper pests. The pesticide has strong knockdown force and quick pesticide effect, can treat thrips and leeches, and is safe to natural enemies of rice planthoppers and spiders.

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the isoprocarb is (1: 60) - (300: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the isoprocarb is (1: 40) - (200: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the isoprocarb is (1: 40) - (100: 1).

(Sumeiwei)

The metolcarb belongs to a quick-acting substituted benzene carbamate pesticide with certain selectivity, has contact poisoning and fumigation effects on pests, also has a certain systemic effect, and has an action mechanism of inhibiting acetylcholinesterase so as to cause paralysis and death of insects.

In a preferred embodiment, the mass ratio of the specific-structure diatomaceous earth to metolcarb is (1: 60) to (200: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the metolcarb is (1: 60) - (150: 1).

In a preferred embodiment, the mass ratio of the diatomaceous earth with the specific structure to the metolcarb is (1: 60) - (100: 1).

(pirimicarb)

The Pirimicarb (Pirimicarb) is a carbamate selective aphicide developed by America Pont-Engineers, has contact, fumigation and systemic effects, has permeability on leaf tissues, and can effectively control aphids resistant to organic phosphorus.

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the pirimicarb is (1: 60) - (200: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the pirimicarb is (1: 60) - (150: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the pirimicarb is (1: 60) - (100: 1).

(methomyl)

The Methomyl is a systemic carbamate pesticide, acts on cholinesterase of pests, destroys nervous systems of the pests and causes the pests to die, has the effects of contact poisoning and stomach poisoning, has strong permeability and a certain ovicidal effect, and also has a good control effect on the pests with resistance to organic phosphorus and pyrethrins.

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the methomyl is (1: 60) - (200: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the methomyl is (1: 60) - (150: 1).

In a preferred embodiment, the mass ratio of the diatomite with the specific structure to the methomyl is (1: 60) - (100: 1).

In some embodiments, other pesticidal active ingredients may be added in appropriate amounts in addition to the preferred active ingredients of the present invention (diatomaceous earth of specific structure and carbaryl/isoprocarb/meta-tolyl/pirimicarb/methomyl), and the present invention is not particularly limited to the other active pesticidal ingredients and may be insecticides, bactericides, herbicides, and the like, which are conventionally added in the art.

As the kind of the conventional insecticide, bactericide, herbicide, specifically, the following may be mentioned.

(insecticides)

BT, CVMP, CVP, CYAP, DDVP, DEP, MEP, MIPC, NAC, PHC, acetamiprid, acephate, oxazaphos, imidacloprid, ethofenprox, clothianidin, chlorthion, cyhalothrin, silafluofen, wireworm, plegia, pleocidin, diazinon, thiamethoxam, thiodicarb, tebufenozide, teflubenzuron, tralomethrin, bifenthrin, pyridaphenthion, fenobucarb, ブルウエルア & ロウカルア, prothiofos, permethrin microcapsules, permethrin, monosulfuron, methomyl, monocrotophos, flupropathrin, fipronil, fluazuron, flufenoxuron, lufenuron, flubencarb, tefluthrin, transfluthrin and the like.

(Bactericide)

TPN, azoxystrobin, iprodione, isoprothiolane, iminoctadine, imazalil, clomazole, carboxin, captan, kresoxim-methyl, dicyclopentenyl, cyproconazole, simeconazole, sulfur hydrate, thiuram, thiophanate-methyl, thifluzamide, tetraconazole, tebuconazole, triadimefon, tolclofos-methyl, triflumizole, triforine, tolclofos-methyl, validamycin A, bitertanol, hydroxyisoxazole, clomeprop, fenarimol, ferimzone, flutolanil, procymidone, propamocarb hydrochloride, propiconazole, benomyl, pencycuron, fosetyl-aluminum, polyoxin D zinc salt, foamectin, mike, milbemycin, metalaxyl, mefenoxamine, organo-copper and the like.

(herbicides)

2,4-PA, CAT, MCP, isopropylamine salt, MDBA, SAP, asulam, glufosinate, alachlor, isoxaben, imazaquin, pyrazosulfuron, ethoxysulfuron, oxadiargyl, metamitron, oryzalin, pyraclonil, tolfen-zachlor, xanthomonas campestris, cyanazine, cyclosulfamuron, dithiopyr, cyclosulfamuron, cinosulfuron, cinmethylin, dimethenamid, triaziflam, triclopyr, trifloxysulfuron sodium, epinapropamide, halosulfuron, bifenox, pyributicarb, butafosinate, butafenin, propafen, penoxsulam, florasulam, trifluofen, pendimethalin, 2-methyl-4-chloropropionate calcium salt, metsulfuron, cyclam, fluroxypyr, thiazopyr and the like.

In addition, in the pesticidal composition of the present invention, an additive effect or a remarkable synergistic effect can be obtained by using the active ingredients (diatomaceous earth of a specific structure and carbaryl/isoprocarb/meta-tolyl-methylcarbamate/pirimicarb/methomyl) in combination with other pesticidal adjuvants.

In some embodiments, the composition is any dosage form prepared by compounding diatomite with a specific structure and a raw drug substance as active ingredients and a pesticide auxiliary agent.

In some embodiments, the pesticide adjuvant is selected from one or more of an adsorbent, a dispersant, a wetting agent, a viscosifier, and a synergist.

In a preferred embodiment, the content of the effective component in the composition is 0.001 to 99.999 wt%.

In a preferred embodiment, the content of the effective component in the composition is 30 to 70 wt%.

In a preferred embodiment, the content of the effective component in the composition is 40 to 60 wt%.

For example, as the pesticide adjuvant, specifically, the following dispersants can be mentioned.

(dispersing agent)

The dispersing agent is styrene polymer; has larger molecular weight and is more effective than small molecular dispersant.

In some embodiments, the dispersant is a sulfonate, polycarboxylate, such dispersant may preferably be KY-R01, KY-F02, both available from Jiangsu Keyuan science and technology, Inc.

In a preferred embodiment, the dispersant is a polymer comprising high molecular weight polyethylene, and such dispersant is most preferably paintable DS-197, available from tianjin hephelfel new materials ltd.

(ii) scribble happiness DS-197, the dispersant is believed to be a mixture comprising a polyether adduct of a high molecular weight polyethylene polymer and a surfactant; possibly, the derivative has characteristic groups similar to the original medicine of the invention, forms anchoring groups with the original medicine of the invention, contains a surfactant with coordination and synergism, reduces the interfacial tension between liquid and liquid or between solid and liquid, ensures that the surface of condensed solid particles is easy to wet, reduces the wetting time, and greatly improves the suspension property of the original medicine substances of the invention, particularly the carbaryl, the isoprocarb and the metolcarb.

In addition, by compounding a certain polyacrylate ethanol copolymer, the suspension property of two raw pesticides, namely pirimicarb and methomyl can be remarkably improved, probably because the polyacrylate ethanol copolymer has a suitable chain structure and has stronger affinity with the two raw pesticides, the characteristic groups are similar and complementary, the force for forming steric hindrance with the base groups is stronger, and the suspension property is not remarkably improved by singly adding DS-197. In the experiment, the polyacrylate ethanol copolymer of the invention is preferably the product with the brand numbers CJ-0581 and CJ-0584, and finally the product obtained when the weight ratio of DS-197: CJ-0581/CJ-0584: the suspension property was the best in (1-2).

In the present invention, in addition to the above-mentioned essential components, other agricultural chemical adjuvants such as an extender, a component stabilizer, a physical property improving agent, etc. which are conventionally used may be added in an appropriate amount as required.

As such a pesticide adjuvant, either solid or liquid; examples of the inorganic fine powder include, but are not limited to, fine mineral powders such as white carbon, clays, calcium carbonate, bentonite, talc, diatomaceous earth, calcium stearate, and colloidal silica, organic or inorganic acid salts such as ammonium sulfate, ammonium hydrogen carbonate, ammonium nitrate, ammonium chloride, potassium chloride, sodium sulfate, magnesium sulfate, sodium citrate, sodium carbonate, and sodium hydrogen carbonate, organic acids such as citric acid and succinic acid, sugars such as sucrose, lactose, and xanthan gum, and water-soluble fine powders such as urea.

In some embodiments, the formulation of the pesticidal composition of the present invention is selected from any one of emulsifiable concentrate, suspension, wettable powder, granule, aqueous agent, poison bait, mother liquor, mother powder.

Among them, wettable powder (wetteapowder) can be produced by air pulverization using a jet mill; the water dispersible powder (Waterdispersablegranule) can be produced by adding water to the wettable powder, kneading, extruding, drying, and granulating.

In a third aspect, the present invention provides a method for preparing a water dispersible powder comprising the pesticidal composition as described above, comprising the steps of: weighing effective components and pesticide adjuvant, and performing high-speed universal pulverization; and adding diatomite with a specific structure into the flask, shaking, transferring the mixture into the flask, stirring and mixing the mixture for 30min by using a stirrer, fully and uniformly mixing the mixture, and then granulating the mixture.

In order to avoid destroying the structure of the Celite610, in the preparation process, mechanical pulverization is not used after the Celite610 is added, but stirring and mixing are carried out; the high-speed universal crushing is carried out by adopting a DTF-50 high-speed universal crusher (Shanghai Xinnuo instruments and equipment Co., Ltd.), and the stirring after adding the diatomite with the specific structure is carried out by adopting a RW20IKA digital display stirrer (Germany IKA group).

In the invention, when the original drug substance in the pesticide composition is any one of carbaryl, isoprocarb, metolcarb, pirimicarb and methomyl, the pesticide composition can be prepared into wettable powder after being prepared; in addition, when the original drug substances in the pesticide composition are isoprocarb and pirimicarb, the original drug substances can be prepared into water dispersible powder, and the two dosage forms can be diluted by water and the like and can be applied to plants, water surfaces or soil. In addition, the preparation can also be used in combination with other bactericides, herbicides, insecticides, fertilizers, soil conditioners, and the like.

The fourth aspect of the invention provides application of the pesticide composition preparation in prevention and treatment of frankliniella occidentalis, trialeurodes vaporariorum, liriomyza sativae, prodenia litura, tea lesser leafhopper, tea yellow thrips, tea geometrid, green plant bug, grape aphid, red spider and scale insect.

The present invention is described in detail below with reference to examples, which are provided for the purpose of further illustration only and are not to be construed as limiting the scope of the present invention, and the insubstantial modifications and adaptations thereof by those skilled in the art based on the teachings of the present invention will still fall within the scope of the present invention.

Example 1

Example 1 diatomaceous earth Celite610 with a particular structure replicates the combined virulence of different pests with different bulk drug substances;

diatomaceous earth, Celite610, a specific structure, gareli, france.

Pests: western flower thrips, whitefly, leaf miner, prodenia litura, tea leafhopper, tea yellow thrips, tea geometrid, green plant bug, grape aphid, red spider and scale insect.

Wherein, the diatomite Celite610 with a specific structure is abbreviated as: t;

when the active compound is carbaryl, the thiodicarb is abbreviated as follows: a;

when the original drug substance is isoprocarb, thiodicarb is abbreviated as: a;

when the original drug substance is meta-tolyl-N-methylcarbamate, thiodicarb is abbreviated as: a;

when the active compound is pirimicarb, thiodicarb is abbreviated as follows: a;

when the active compound substance is methomyl, thiodicarb is abbreviated as follows: a;

t: A ═ (200: 1) indicates that the mass ratio of T to A is 200: 1;

t: A ═ (100: 1) indicates that the mass ratio of T to A is 100: 1;

t: A ═ (50: 1) indicates that the mass ratio of T to A is 50: 1;

t: A ═ (1: 1) indicates that the mass ratio of T to A is 1: 1;

t: A ═ (1: 20) indicates that the mass ratio of T to A is 1: 20;

t: A ═ (1: 30) indicates that the mass ratio of T to A is 1: 30;

t: A ═ (1: 40) indicates that the mass ratio of T to A is 1: 40;

t: A (1: 60) means that the mass ratio of T to A is 1: 60.

(1) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the carbaryl on the Frankliniella occidentalis.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf-soaking dish method is adopted. Beating the blades into leaf disks by a puncher with the diameter of 18mm, soaking the leaf disks in a test reagent solution for 10 seconds, putting the leaf disks into a culture dish (with the diameter of 90mm) padded with moisturizing filter paper, putting 5 leaf disks into each dish, naturally drying, then selecting the western flower thrips into the culture dish, sealing the mouth of the dish by a preservative film, pricking some small holes on the film by a needle for ventilation, repeating the treatment for 4 times, finishing the treatment, and placing the dish in an observation room for culture.

And (4) after 72h, checking and recording the death condition of the test insects, wherein the death judgment standard of the test insects is that the test insects are dead as the result of no response when the pointed writing brush is lightly contacted with the test insects.

The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 indicates an antagonistic effect, and between 80 and 120 indicates an additive effect. The co-toxicity coefficient is calculated as follows

Theoretical Toxicity Index (TTI) of the mixture of agent A (toxicity index × A) and agent B (toxicity index × B)

TABLE 1 toxicity test results of Celite610 and carbaryl mixture on Frankliniella occidentalis

(2) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the carbaryl on the whitefly.

According to the method in NY/T1154.14-2008 standard, a leaf soaking method is adopted, agar (1%) liquid is heated and dissolved, the agar is sucked into beakers with the size of 20m L-200 m L, the beakers are condensed at normal temperature for standby use, leaves are soaked in test solution for 10 seconds, the leaves are taken out and naturally dried, the leaves are adhered to the surface of solidified agar liquid, leaf stalks are wrapped by wet cotton balls, 3-4 leaves are placed in each beaker, whitefly adults are inoculated, the number of the heads of each beaker is about 15, then the mouth of each beaker is sealed by a preservative film, a needle is used for pricking a plurality of small holes on the film to ventilate, the treatment is repeated for 4 times, and the beaker is placed in an observation room for culture after the treatment is finished.

And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the tweezers lightly contact the test insects.

The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006.

TABLE 2 toxicity test results of Celite610 and carbaryl mixture against Bemisia alba

(3) Co-toxicity coefficient (CTC) screening: combined virulence assay for Celite610 and carbaryl against liriomyza sativae.

Referring to the method in NY/T1154.13-2008 standard, spraying method is adopted. Transplanting potted cowpea pest-free seedlings into pest-raising cages, inoculating a certain amount of mated adult liriomyza huidonii, taking out bean seedlings with liriomyza huidonii eggs after 6h, placing the bean seedlings in another pest-raising cage, continuously raising the bean seedlings to 2-year larvae (ensuring that each disc contains no less than 80 larvae of 2-year larvae), then carrying out spraying treatment, naturally air drying, and transferring the bean seedlings to an artificial climate box for continuous raising. Each treatment was repeated 4 times and a blank control was run.

And (3) cutting bean seedlings from roots at the later stage of 3-year-old larvae, moisturizing the tail ends by using soaked cotton balls, putting the bean seedlings into plastic basins in an artificial climate box, pupating the bean seedlings, and counting the quantity of pupae in each dish.

TABLE 3 toxicity test results of Celite610 and carbaryl in combination for Liriomyza sativae

(4) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and carbaryl on prodenia litura.

Referring to the method in NY/T1154.14-2008 standard, the leaf-dipping dish method is adopted. Beat the cabbage leaf into the leaf dish with the hole puncher of diameter 18mm, dip the leaf dish in the liquid of testing medicine for 10 seconds after, put into the culture dish (diameter 90mm) that has filled with the filter paper that moisturizes, 6 leaf dishes are put into to every dish, wait to dry the back naturally, select again and starve 4h twill noctuid 3 age larva to the culture dish in, every dish is not less than 10, 4 times are repeated, the processing finishes, covers the ware lid, arranges the indoor culture of observation in.

TABLE 4 toxicity test results of mixing Celite610 and carbaryl against spodoptera litura

(5) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and carbaryl on the tea leafhopper.

According to the method in NY/T1154.11-2008 standard, a dipping method is adopted, 3 fresh tea tree sprout tips are selected, the tea tree sprout tips are dipped in a test liquid medicine for 30 seconds, taken out and naturally dried, wet absorbent cotton is used for wrapping the base parts of the sprout tips for moisturizing, absorbent cotton balls are sealed by a preservative film and placed in a glass test tube (phi 200 × 30mm), the concentration is repeated for 4 times, then a writing brush is used for inoculating 3-year-old nymphs of tea lesser leafhoppers subjected to ether mild anesthesia treatment, 15 +/-2 heads of each tube are used, the mouth of the test tube is sealed by white gauze, the treatment is finished, and the tea tree sprouts are placed in an observation room.

TABLE 5 toxicity test results of Celite610 and carbaryl mixed on tea leafhopper

(6) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and carbaryl on the tea leaf thrips.

According to the standard method NY/T1154.14-2008 of biological detection, a leaf soaking method is adopted, 3 tea tree sprout tips with a certain amount of tea thrips tabaci (no less than 20) are selected, soaked in a test liquid for 10 seconds, taken out and naturally dried, wet absorbent cotton is used for wrapping the base parts of the sprout tips for moisturizing, absorbent cotton balls are sealed by preservative films and placed in a glass test tube (phi 200 × 30mm), the process is repeated for 4 times per concentration, the mouth of the test tube is sealed by the preservative films, the small holes are punched for ventilation, and the test tube is placed in an observation room after being treated.

And after 72h, examining the death condition of the test insects under a dissecting mirror, and recording, wherein the death judgment standard of the test insects is that the test insects die because the test insects are not reacted when the pointed writing brush is lightly contacted with the test insects.

TABLE 6 toxicity test results of mixture of Celite610 and carbaryl on tea leaf thrips tabaci

(7) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and carbaryl on tea geometrid.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. Selecting 3 fresh tea tree sprouts, soaking in the test liquid medicine for 30 seconds, taking out, naturally drying, wrapping the bases of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a can bottle, wherein the concentration is repeated for 4 times. Then inoculating 2-year-old nymphs of tea geometrid with consistent size and liveness and health into a brush pen, wherein 15 +/-2 heads of each pot are sealed by white gauze. After the treatment, the sample is placed in an observation chamber.

Table 7.Celite610 and carbaryl mixed toxicity test results on tea leaf geometrid

(8) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and carbaryl on the green plant bug.

Referring to the method in NY/T1154.11-2008 standard, a leaf spray method was used. The method comprises the steps of disinfecting, cleaning and airing fresh kidney beans by adopting an immersion method, cutting the kidney beans into 2-3 cm segments, immersing the kidney beans in liquid medicines with different concentrations for 30 seconds, airing the kidney beans under natural conditions, and then putting the kidney beans into transparent plastic boxes (6 cm in diameter and 7cm in height) with smooth air, wherein each box has two sections. Healthy and consistent 3-year-old nymphs of lygus lucorum were inoculated, 15 or so per box, repeated 4 times, and a blank control was set. And putting the plastic box into a light incubator for culturing.

And after 48h, the death condition of the test insects in each box is checked, the bodies of the lygus lucorum are lightly touched by the brush tips, and the death judgment standard of the test insects is that no response is calculated when the tweezers lightly touch the test insects.

Table 8. Combined virulence determination of green plant bug by Celite610 and carbaryl combination

(9) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the compounded T and carbaryl on grape aphids.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Cutting leaves with a certain amount of grape aphids, soaking in the liquid medicine to be tested for 10 seconds, taking out, absorbing the liquid medicine around the insect body with absorbent filter paper, placing in a culture dish padded with moisturizing filter paper, moisturizing with a wet cotton ball, and covering the culture dish with wet gauze. Each 30 aphids were treated, 4 replicates and a blank treatment without drug (including solvent and emulsifier used) was used. After the treatment, the cells were transferred to an observation room for cultivation.

And (3) detecting the death condition of the test insects after 48h, and recording, wherein the judgment standard of the death of the test insects is as follows: the aphids were lightly touched with a writing brush, and the body was judged dead.

TABLE 9 toxicity test results of mixture of Celite610 and carbaryl on grape aphid

(10) Co-toxicity coefficient (CTC) screening: combined virulence determination of frankliniella occidentalis by Celite610 formulated with isoprocarb.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf-soaking dish method is adopted.

TABLE 10 toxicity assay results of Celite610 and isoprocarb mixture on Frankliniella occidentalis

(11) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the isoprocarb on the whitefly.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted.

TABLE 11 toxicity test results of Celite610 and isoprocarb mixture against Bemisia alba

(12) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and isoprocarb on the tea leafhoppers.

Referring to the method in NY/T1154.11-2008 standard, the dipping method is adopted.

TABLE 12 toxicity test results of Celite610 and isoprocarb mixture on tea leafhopper

(13) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the isoprocarb on the tea leaf thrips.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf dipping method is adopted.

TABLE 13 toxicity test results of mixture of Celite610 and isoprocarb on tea leaf thrips

(14) Co-toxicity coefficient (CTC) screening: combined virulence assay of green plant bug by Celite610 formulated with isoprocarb.

Referring to the method in NY/T1154.11-2008 standard, a leaf spray method was used.

Table 14 toxicity assay results for apolygus lucorum with Celite610 and isoprocarb mixture

(15) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the isoprocarb on grape aphid.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted.

TABLE 15 toxicity assay results for grape aphid with Celite610 and isoprocarb mixture

(16) Co-toxicity coefficient (CTC) screening: the combined toxicity of the Celite610 and the metolcarb compound on the frankliniella occidentalis is determined.

TABLE 16 toxicity test results of Celite610 and metolcarb mixture on Frankliniella occidentalis

(17) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the metolcarb compound to the trialeurodes vaporariorum.

TABLE 17 toxicity test results of Celite610 and metolcarb mixture against Bemisia alba

(18) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and the metolcarb compound on the tea leafhopper.

TABLE 18 toxicity test results of Celite610 and metolcarb mixture on tea leafhoppers

(19) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the metolcarb compound on the tea leaf thrips.

TABLE 19 toxicity test results of mixture of Celite610 and metolcarb on tea leaf thrips tabaci

(20) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the metolcarb compound to the green plant bug.

TABLE 20 toxicity assay results for green plant bug by Celite610 and metolcarb mixture

(21) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the metolcarb compound on the grape aphid.

TABLE 21 toxicity assay results for grape aphid with Celite610 and metolcarb combination

(22) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the pirimicarb to the trialeurodes vaporariorum.

TABLE 22 toxicity test results of Celite610 and pirimicarb mixture against Bemisia tabaci

(23) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and pirimicarb on the tea leafhopper.

TABLE 23 toxicity test results of mixture of Celite610 and pirimicarb against tea leafhopper

(24) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and the pirimicarb on the green plant bug.

Table 24.Celite610 and pirimicarb mixture toxicity test results for green plant bug

(25) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the pirimicarb on the grape aphid.

TABLE 25 toxicity test results of mixture of Celite610 and pirimicarb against aphid on grape

(26) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and the methomyl compound to citrus red spiders.

According to the method in NY/T1154.13-2008 standard, a leaf spraying method is adopted, smooth citrus leaves with the same size are cleaned and wiped dry, the leaves are attached to wet sponges with the leaf surfaces facing upwards, moist small cotton slivers are used for surrounding the leaves to prevent tested mites from escaping, the wet sponges are placed into a culture dish with the diameter of 9cm, water is added into the culture dish, the water level is slightly lower than the height of the sponges, a plurality of indoor raised mites are inoculated onto the leaves, each leaf is not lower than 30 heads, the culture dish is placed on a bottom plate of a Potter spraying tower to be sprayed, the spraying amount is 1m L, liquid medicine is taken out after being settled for 1min, the liquid medicine is transferred to raising conditions, the raising is carried out for 4 times, and blank control is arranged.

After 72h, the death of the test insects was examined under a dissecting mirror and recorded. The judgment standard of the death of the test insects is as follows: death was counted as no response when the test insects were gently touched by tweezers.

TABLE 26 toxicity assay results for citrus red spiders with Celite610 and Mieduowei mixture

(27) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and methomyl for the citrus icerya.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Dipping the trimmed citrus leaves with citrus arrowhead scales in a test solution for 10 seconds, taking out the citrus leaves, absorbing redundant liquid medicine around the leaves and the insects by using filter paper, putting the citrus leaves and the insects into a culture dish (with the diameter of 90mm) filled with moisturizing filter paper, wrapping petioles by using wet cotton balls for moisturizing, repeating the steps for 4 times per concentration, covering the dish cover after the treatment is finished, and placing the dish cover in an observation room for culturing.

After 72h, examining the death condition of the test insects by using a dissecting mirror, and recording, wherein the death judgment standard of the test insects is fresh and alive, and the test insects are regarded as live insects in yellow or light yellow; the dead insects are considered to be dead insects if the body of the insects is shriveled and the color is dark.

TABLE 27 toxicity assay results for Celite610 and methomyl combinations against Cucumaria citricola (Cekukay)

(28) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the methomyl on the Frankliniella occidentalis.

TABLE 28 toxicity test results of Celite610 and methomyl mixture on Frankliniella occidentalis

(29) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the methomyl compound to the trialeurodes vaporariorum.

TABLE 29 toxicity test results of Celite610 and methomyl mixture against Bemisia alba

(30) Co-toxicity coefficient (CTC) screening: combined virulence assay for Celite610 and methomyl paired liriomyza sativae.

Referring to the method in NY/T1154.13-2008 standard, spraying method is adopted.

TABLE 30 toxicity test results of Celite610 and Mieduowei mixed Papyria

(31) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and methomyl to prodenia litura.

Referring to the method in NY/T1154.14-2008 standard, the leaf-dipping dish method is adopted.

TABLE 31 toxicity test results of Celite610 and methomyl mixture against prodenia litura

(32) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and the methomyl compound on the tea leafhoppers.

TABLE 32 toxicity test results of Celite610 and methomyl mixture on tea leafhoppers

(33) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the methomyl compound on the tea leaf thrips.

TABLE 33 toxicity test results of mixture of Celite610 and methomyl on tea leaf thrips

(34) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and the methomyl compound on tea geometrid.

TABLE 34 toxicity test results of Celite610 and Mieduowei mixture for tea geometrid

(35) Co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and the methomyl compound on the green plant bug.

TABLE 35 toxicity test results of Celite610 and methomyl mixture on green plant bug

(36) Co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and the methomyl on grape aphids.

TABLE 36 toxicity test results of Celite610 and methomyl mixture on grape aphid

Examples 2 to 16

Embodiments 2 to 16 provide a novel pesticidal composition, which comprises, by mass, 25% of diatomaceous earth having a specific structure, 25% of a raw material, 15% of a dispersant, and white carbon black to make up to 100%.

Wherein, the diatomite with the specific structure is Celite610 of the France Yirui stone company; the dispersants are of different kinds; white carbon black was purchased from taida chemical technology ltd;

according to the designed formula, the dispersing agent, the white carbon black and the original drug substance are subjected to high-speed universal grinding, so that the dispersing agent, the white carbon black and the original drug substance can be uniformly mixed with each other, and the fineness is reduced; then adding the Celite610, and in order to avoid destroying the structure of the Celite610, mechanical crushing is not used; then shaking, transferring into a flask after 10min, stirring and mixing for 30min by using a stirrer, and fully and uniformly mixing to obtain the wettable powder.

(1) Determination of the wetting time: the wettable powder is poured into a beaker containing a certain amount of standard hard water from a specified height and the time for complete wetting is measured according to the method of GB/T5451-2001.

(2) Measurement of suspension percentage: the standard hard water is used for preparing a sample to be tested into suspension liquid according to the method of GB/T14825-2006. Under the specified conditions, after standing in a measuring cylinder, measuring the mass fraction of the effective components in the suspension at the bottom of one tenth of the measuring cylinder, and calculating the suspension rate.

TABLE 16 wetting time, suspension percentage of different dispersants

In the above table, the amount refers to the total amount of the dispersant, and when the dispersant is a single substance, the amount is the amount of the substance itself, and when the dispersant is a complex of multiple substances, the amount is the total amount of the complex mixture; a represents that a technical substance is carbaryl, b represents that the technical substance is pirimicarb, c represents that the technical substance is methomyl, d represents that the technical substance is methomyl, and Celite610 is replaced by common silicon dioxide with the particle size of 5-7 mu m; DS-197 shorthand DS; CJ-0581 abbreviation 0581; CJ-0584 abbreviation 0584; GY-D06 was purchased from Guangyi agricultural chemistry, Inc. of Beijing; KY-F01 and SOPA-270 were purchased from Jiangsu Kaiyuan technology Co.

Claims

1. A novel insecticidal pesticide composition is characterized by comprising diatomite with a specific structure and a raw pesticide substance as effective components, wherein the mass ratio of the diatomite with the specific structure to the raw pesticide substance is (1: 60) - (300: 1); the technical material is selected from any one of carbaryl, isoprocarb, metolcarb, pirimicarb and methomyl.

2. A novel pesticidal and pesticidal composition according to claim 1, wherein the diatomaceous earth of specific structure has a zigzag honeycomb structure.

3. A novel pesticidal pesticide composition according to claim 2, wherein the mass ratio of the specific-structure diatomaceous earth to carbaryl is from (1: 60) to (300: 1).

4. A novel pesticidal and pesticidal composition according to claim 2, wherein the mass ratio of the diatomaceous earth of specific structure to isoprocarb is (1: 60) to (300: 1).

5. A novel pesticidal pesticide composition according to claim 2, wherein the mass ratio of the specific-structure diatomaceous earth to the metolcarb is (1: 60) to (200: 1).

6. A novel pesticidal and pesticidal composition according to claim 2, wherein the mass ratio of the specific-structure diatomaceous earth to pirimicarb is (1: 60) to (200: 1).

7. A novel pesticidal pesticide composition according to claim 2, wherein the mass ratio of the specific-structure diatomaceous earth to methomyl is (1: 60) to (200: 1).

8. A novel pesticidal and pesticidal composition according to any one of claims 1 to 7, which is in any form of a formulation comprising a specific-structure diatomaceous earth and a raw material substance as active ingredients, and a pesticidal auxiliary.

9. A novel pesticidal and pesticidal composition according to claim 8, wherein the formulation is any one selected from the group consisting of emulsifiable concentrate, suspension concentrate, wettable powder, dust, granule, aqueous agent, poison bait, mother liquor and mother powder.

10. A novel pesticidal composition according to claim 9, wherein the content of the active ingredient in the composition is 0.001 to 99.999% by weight.