CN111264538A

CN111264538A - Efficient insecticidal and acaricidal composition and application thereof

Info

Publication number: CN111264538A
Application number: CN202010114188.8A
Authority: CN
Inventors: 刘永山
Original assignee: Guangxi Yicai Agricultural Development Co Ltd
Current assignee: Guangxi Yicai Agricultural Development Co Ltd
Priority date: 2020-02-25
Filing date: 2020-02-25
Publication date: 2020-06-12

Abstract

The invention relates to the technical field of pesticides, and provides a high-efficiency insecticidal and acaricidal composition which comprises raw materials of diatomite with a saw-tooth honeycomb structure and raw pesticide in a weight ratio of (1: 40) - (400: 1); the technical material is at least one of clofentezine and indoxacarb.

Description

Efficient insecticidal and acaricidal composition and application thereof

Technical Field

The invention relates to the technical field of pesticides, in particular to an insecticidal and acaricidal composition with high efficiency and application thereof.

Background

In recent years, with the dramatic increase in the number of pest mite populations, acarids have been rising as main control targets of pest (mite) damage on crops such as fruit trees, cotton, vegetables, rice, wheat, tea trees, tobacco and the like. When harmful mites occur, the acaricide is the most effective means for preventing and controlling the harmful mites, and currently, pesticides registered in the department of agriculture for preventing and controlling the agricultural harmful mites mainly comprise abamectin, pyridaben, propargite, fenbutatin oxide and the like, and the harmful mites have resistance to the pesticides, so that the development of a safe and pollution-free pesticide capable of effectively preventing and controlling the harmful mites is urgently needed.

Some scientists believe that the clogging of insect stomata and trachea with diatomite particles is the cause of death. Chiu crystal SiO for grain elephant and bean elephant₂No difference in oxygen consumption of adults was observed after the treatment with the bentonite powder, indicating that SiO is present₂The particles did not enter the insect trachea. Later, scientists have theorized that diatomaceous earth insecticides disrupt the insect cuticle. Mews experiments on the adult tenebrio molitor with the diatomite insecticide, and observation through an electron microscope shows that after the treatment of the diatomite insecticide, the wax layer of the adult tenebrio molitor epidermis is damaged, even a small amount of diatomite is embedded on the epidermis, and other uses the diatomite insecticide HomeShield to treat the pupa of the tenebrio molitor, and the weight of the treated pupa is obviously lighter than that of a control pupa after weighing, so that the water loss phenomenon of the treated pupa of the tenebrio molitor is proved.

At present, the pesticide of the compound mixture is selected to become the current first choice and an effective way, and the best synergistic effect is obtained by adjusting the mixture ratio of different pesticides to obtain the pesticide with complementary performance. However, when the active ingredients of the raw medicines are applied, the application effect is reduced because the content of the auxiliary agents in the formula is low.

Disclosure of Invention

In order to solve the technical problems, the invention provides a high-efficiency insecticidal and acaricidal composition, which comprises raw materials of saw-tooth cellular structure diatomite and raw pesticide in a weight ratio of (1: 40) - (400: 1); the technical material is at least one of clofentezine and indoxacarb.

As a preferred technical scheme, the technical material is clofentezine; the weight ratio of the diatomite and the clofentezine in the sawtooth-shaped honeycomb structure is (1: 40) - (100: 1).

As a preferable technical scheme, the application performance of the insecticidal and acaricidal composition is selected from one of missible oil, suspending agent, wettable powder, water dispersible granules and water aqua.

A wettable dispersant containing the insecticidal and acaricidal composition comprises the following raw materials in percentage by weight: 10-50% of the insecticidal and acaricidal composition, 5-10% of an auxiliary agent and a first carrier for complementing the balance; the auxiliary agent comprises at least one of a first wetting agent and a first dispersing agent.

As a preferable technical solution, the weight ratio of the first wetting agent to the first dispersing agent is 1: (0.5-2.5); the first wetting agent is selected from at least one of alkyl naphthalene sodium sulfonate, diisopropyl naphthalene sodium sulfonate, alkyl naphthalene sulfonate, sodium dodecyl benzene sulfonate and sodium dodecyl sulfate; the first dispersant is at least one selected from ammonium lignosulfonate, polycarboxylic acid dispersant, NNO, MF, CNF, EO/PO block polyether and aralkyl phenol polyoxyethylene ether phosphate.

As a preferred technical solution, the first dispersant in the present invention is a combination of ammonium lignosulfonate and polycarboxylic acid dispersant; the weight ratio of the ammonium lignosulphonate to the polycarboxylic acid dispersant is (0.1-0.5): 1.

the water dispersible granule containing the insecticidal and acaricidal composition at least comprises the following raw materials in percentage by weight: 5-40% of the insecticidal and acaricidal composition, 0.5-20% of a disintegrating agent, 5-15% of a binder, 5-15% of other auxiliary agents and a second carrier for balancing; the other auxiliary agent comprises at least one of a second wetting agent and a second dispersing agent.

In a preferred embodiment, the binder in the present invention includes at least one of polyacrylamide, starch, dextrin, gum arabic, soy protein, zein, bone glue, gelatin, sodium silicate, shellac, polyvinyl acetate, polyvinyl alcohol, and sodium polyacrylate.

As a preferred technical solution, the polyacrylamide in the present invention is cationic polyacrylamide.

The fourth aspect of the invention provides an application of the insecticidal and acaricidal composition, which is applied to control at least one of red spiders, frankliniella occidentalis, whiteflies, liriomyza sativae, prodenia litura, tea lesser leafhopper, tea yellow thrips, tea geometrid, green plant bugs and grape aphids.

The foregoing aspects, features and advantages of the invention will be more readily appreciated with reference to the following detailed description.

Drawings

FIG. 1 is an optical microscope photograph of a saw-tooth diatomaceous earth according to the present invention.

FIG. 2 is an electron microscope image of the saw-toothed diatomaceous earth of the present invention.

Detailed Description

The technical features of the technical solutions provided by the present invention will be further clearly and completely described below with reference to the specific embodiments, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

The words "preferred", "preferably", "more preferred", and the like, in the present invention, refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.

The invention provides a high-efficiency insecticidal and acaricidal composition, which comprises the raw materials of diatomite with a zigzag honeycomb structure and raw pesticide in a weight ratio of (1: 40) - (400: 1); the technical material is at least one of clofentezine and indoxacarb.

In the present invention, the term "composition" means that the components of the composition are packaged in combination or separately and used together when used. In the invention, the 'diatomite with a sawtooth-shaped honeycomb structure and raw pesticide' refers to: the diatomite with the sawtooth-shaped honeycomb structure and the original medicine can be mixed and packaged together and used together when in use; further comprising: the diatomite with the sawtooth-shaped honeycomb structure and the original medicine are separately packaged and are mixed together according to the proportion when in use.

Sawtooth-shaped diatomite

Diatomaceous earth is deposited from remains of unicellular aquatic algae plants. Forming a diatomite ore bed under certain geological conditions. The diatomite has the characteristics of porosity, large specific surface area, low density, good adsorbability, acid resistance, heat resistance and the like, and the main component of the diatomite is amorphous SiO₂。

The diatomite with the specific structure is diatomite with the specific crystal structure, and the general diatomite contains a small amount of Al besides the main component of silicon dioxide₂O₃、Fe₂O₃CaO, MgO, etc., and organic matter. The diatomite with a specific structure is diatomite with a specific sawtooth-shaped honeycomb structure silicon dioxide content after being purified.

In some embodiments, the silica having a specific saw-tooth honeycomb structure may be selected from diatomaceous earth having a specific structure of Celite610, available from garnets, france. Under an optical microscope and an electron microscope, the Celite610 has a clear zigzag honeycomb structure, as shown in fig. 1 and fig. 2. On one hand, the diatomite with the specific structure has a larger specific surface area due to the saw-toothed honeycomb structure, and is favorable for being better adsorbed on a waxy layer on the body surface of the insect, so that the insect body is quickly dehydrated. On the other hand, due to the zigzag honeycomb structure, the composition is beneficial to causing damage to a wax layer on the surface of the insect body, tearing the surface of the insect body, rapidly permeating into the insect body and causing fatal damage to the insect, thereby improving the lethality of the composition.

Clofentezine

Clofentezine, common english name, molecular formula: C14H8C12N4, belonging to a contact-type organic nitrogen acaricide with low toxicity. Clofentezine belongs to an embryo development inhibitor, mainly kills mite eggs, but also has a certain effect on young mites and is ineffective on adult mites. The effective components and the preparation thereof are stable to light, air and heat and have low flammability. In Spain, Israel, Chilean and New Zealand for controlling mites on apple and other fruit tree crowns. The pesticide is sprayed before winter eggs hatch in an orchard or a vineyard, and can prevent and control phytophagous spider mites in the whole season. In a four-year field test, the composition can prevent and control panonychus ulmi from apple and peach trees. In a word, the pesticide has special effect on panonychus ulmi (apple red mite), has long lasting period, is mainly used as an ovicide, has certain control effect on juvenile mites, has no influence on predatory mites and beneficial insects, and is applied to apples, ornamental plants, peas, oranges and cottons once before and after the flowering period.

In some embodiments, the prodrug is clofentezine; the weight ratio of the diatomite and the clofentezine with the sawtooth-shaped honeycomb structure is (1: 40) - (100: 1); preferably, the weight ratio of the diatomite with the saw-tooth honeycomb structure to the clofentezine is (1: 1) - (100: 1); more preferably, the weight ratio of the diatomite and the clofentezine in the sawtooth-shaped honeycomb structure is 50: 1.

in the invention, on one hand, the diatomite can be adsorbed to the epidermis of the insect, the wax layer of the epidermis plays a role in preventing water loss in the body, and after the wax layer is damaged by the diatomite, the water in the body of the insect can be excessively lost to cause death of the insect for grain storage insects, and on the other hand, the diatomite has strong killing power on mite eggs and also has strong killing power on young mites and nymphs. Can inhibit the oviposition amount of adult mites and the oviposition hatching rate, has long lasting period which can reach 50 to 60 days generally. But clofentezine has poor effect on adult mites and quick action, can achieve the highest acaricidal activity only 2 weeks after being generally applied, and cannot meet the control requirements on harmful mites when being used alone. However, the inventor unexpectedly finds that the clofentezine is compounded between the specific diatomite with the sawtooth-shaped honeycomb structure to generate an excellent synergistic interaction effect, so that the death of pests can be accelerated, and the insecticidal effect of the clofentezine on adult mites can be improved.

Indoxacarb

Indoxacarb (indoxacarb) is a latest high-efficiency pesticide, can prevent and control various pests on crops such as grains, cotton, fruits, vegetables and the like by blocking sodium ion channels in nerve cells of insects to disable nerve cells, has a contact poisoning stomach poisoning effect, has excellent insecticidal activity on nearly all important agricultural lepidoptera pests such as cotton bollworms, tobacco budworms, plutella xylostella, cabbage caterpillars, beet armyworms, cabbage loopers, codling moths and the like, and also has a certain effect on parts of homoptera and coleoptera pests such as lesser leafhoppers, potato leafhoppers, peach aphids, potato beetles and the like.

In some embodiments, the prodrug is indoxacarb; the weight ratio of the diatomite and the indoxacarb with the sawtooth-shaped honeycomb structure is (1: 40) - (100: 1).

In some embodiments, the weight ratio of the diatomite and the indoxacarb with the zigzag honeycomb structure is 200: 1.

in some embodiments, the weight ratio of the diatomite and the indoxacarb with the zigzag honeycomb structure is 1: 20.

in some embodiments, the weight ratio of the diatomite and the indoxacarb with the zigzag honeycomb structure is 1: 1.

in the invention, the inventor finds that indoxacarb has a unique insecticidal effect, is rapidly converted into DCJW in an insect body, acts on an inactivated voltage-gated sodium ion channel of insect nerve cells by the DCJW, irreversibly blocks nerve impulse transmission in the insect body, destroys the nerve impulse transmission, and causes the pests to have dyskinesia, failure to eat, paralysis and finally death. The indoxacarb and the diatomite with the sawtooth-shaped honeycomb structure are compounded for use, the killing power of the indoxacarb on insects can be further enhanced, and the inventor thinks that the diatomite with the sawtooth-shaped honeycomb structure breaks the body surface of the insects, so that the penetrating power of the indoxacarb on the body surface of the insects is accelerated, and the maladjustment on insect sodium ion channels and the damage effect on a nervous system are improved.

In some embodiments, the insecticidal and acaricidal composition has an application property selected from one of emulsifiable solution, suspending agent, wettable powder, water dispersible granule and water aqua.

The second aspect of the invention provides a wettable dispersant containing the insecticidal and acaricidal composition, which comprises the following raw materials in percentage by weight: 10-80% of the insecticidal and acaricidal composition, 5-10% of an auxiliary agent and a first carrier to make up the balance; the auxiliary agent comprises at least one of a first wetting agent and a first dispersing agent.

In some embodiments, the wettable dispersant containing the insecticidal and acaricidal composition comprises the following raw materials in percentage by weight: 40-60% of the insecticidal and acaricidal composition, 6-8% of an auxiliary agent and a first carrier for complementing the balance; the adjuvant comprises a combination of a first wetting agent and a first dispersant.

In some embodiments, the wettable dispersant containing the insecticidal and acaricidal composition comprises the following raw materials in percentage by weight: 50% of the insecticidal and acaricidal composition, 7% of an auxiliary agent and a first carrier to make up the balance; the adjuvant comprises a combination of a first wetting agent and a first dispersant.

In some embodiments, the weight ratio of the first wetting agent to the first dispersant is 1: (0.5-2.5); preferably, the weight ratio of the first wetting agent to the first dispersing agent is 1: (1-2); more preferably, the weight ratio of the first wetting agent to the first dispersing agent is 1: 1.5.

in the invention, the first wetting agent and the second wetting agent are the same wetting agent; the first dispersant and the second dispersant are the same dispersant; the first carrier and the second carrier are the same carrier.

In some embodiments, the first wetting agent is selected from at least one of sodium dibutylnaphthalene sulfonate, sodium diisopropylnaphthalene sulfonate, alkylnaphthalene sulfonate, sodium dodecylbenzene sulfonate, sodium lauryl sulfate; the first dispersant is at least one selected from ammonium lignosulfonate, polycarboxylic acid dispersant, NNO, MF, CNF, EO/PO block polyether and aralkyl phenol polyoxyethylene ether phosphate.

In some preferred embodiments, the first wetting agent is selected from the group consisting of sodium dibutylnaphthalenesulfonate.

In some preferred embodiments, the first dispersant is a combination of ammonium lignosulfonate, polycarboxylic acid dispersant; the weight ratio of the ammonium lignosulphonate to the polycarboxylic acid dispersant is (0.1-0.5): 1; preferably, the weight ratio of the ammonium lignosulfonate to the polycarboxylic acid dispersant is 0.3: 1.

in some preferred embodiments, the polycarboxylic acid dispersant of the present invention is purchased from the GY-D series of widely available agricultural chemical company, Inc. of Beijing; more preferably, the polycarboxylic acid dispersant is polycarboxylate dispersant GY-D06.

In some embodiments, the first carrier is selected from at least one of kaolin, precipitated calcium carbonate, bentonite, activated clay, diatomaceous earth, white carbon black, attapulgite, clay, montmorillonite, pumice, china clay, quicklime, magnesium lime, phosphorus lime, dolomite, gypsum, sepiolite, mica; preferably, the first carrier is kaolin.

In some embodiments, the kaolin has a mesh size of 1000-; preferably, the kaolin has a mesh number of 1500 meshes.

In the invention, when the wettable dispersant or the water dispersible granule is used, the dosage of the auxiliary agent of the formula is relatively less, and the wettability and the spreading performance of the diluted wettable dispersant or the diluted water dispersible granule to crops and targets are relatively poor, but the inventor finds that when the auxiliary agent is the ammonium lignosulfonate, the polycarboxylic acid dispersant and the sodium dibutylnaphthalenesulfonate in specific dosage, the synergistic effect on the insecticidal effect can be achieved, the wetting and spreading performance of the liquid medicine to the crops and the targets can be enhanced, and the insecticidal efficiency is further improved aiming at the compound of the diatomite with the saw-tooth honeycomb structure and the clofentezine or the compound of the diatomite with the saw-tooth honeycomb structure and the indoxacarb. Particularly, when the polycarboxylate dispersant GY-D06 is selected as the dispersant, the effect is more excellent, and the inventor believes that the effect is probably because, in the invention, the polycarboxylate dispersant GY-D06 has more active groups on the main chain, can generate acting force with the diatomite with a large-size saw-tooth honeycomb structure to stabilize the existence of the diatomite, and the sodium dibutylnaphthalene sulfonate is a small molecular monomer with a double straight-chain alkyl structure, can interact with the diatomite, can increase the steric hindrance of the diatomite to prevent the aggregation, and has good flexibility as the side chain structure of the butyl of the sodium dibutylnaphthalene sulfonate, the method can improve the fluidity of the diatomite in the solvent, further ensure the dispersibility of the system, reduce the dispersibility of the liquid drops when in use, and is beneficial to improving the target wettability to insects.

A preparation method of a wettable dispersant containing the insecticidal and acaricidal composition at least comprises the following steps:

weighing the components, crushing the auxiliary agents to enable the auxiliary agents to be mixed with each other, adding the original medicine, stirring and mixing again, finally adding the sawtooth-shaped diatomite, shaking and mixing in a sealed mode, then transferring into a container, and stirring and fully mixing by using a stirrer.

In the invention, according to the design formula, proper auxiliary agents are weighed and are subjected to high-speed universal grinding, so that the auxiliary agents can be uniformly mixed with each other, and the fineness is reduced. The specific structure diatomaceous earth is then added without mechanical crushing to avoid destroying the structure of the specific structure diatomaceous earth. The applicant speculates that the polycarboxylic acid dispersant and the sodium dibutylnaphthalenesulfonate are selected for compounding, certain acting force is generated between the polycarboxylic acid dispersant and the diatomite during mixing, and the special comb-shaped structure of the polycarboxylic acid dispersant and the dialkyl direct connection with the special proper length of the sodium dibutylnaphthalenesulfonate not only have certain wetting and dispersing effects on the diatomite, but also can reduce certain protection effects on the tooth-shaped structure of the diatomite during the blending stage, so that the tooth-shaped diatomite can fully exert the effects on the body surface of a tearing insect.

The third aspect of the invention provides a water dispersible granule containing the insecticidal and acaricidal composition, which comprises the following raw materials in percentage by weight: 5-60% of the insecticidal and acaricidal composition, 0.5-20% of a disintegrating agent, 5-15% of a binder, 5-15% of other additives and a second carrier for balancing; the other auxiliary agent comprises at least one of a second wetting agent and a second dispersing agent.

In some preferred embodiments, the water dispersible granule containing the insecticidal and acaricidal composition comprises at least the following raw materials in percentage by weight: 45% of the insecticidal and acaricidal composition, 12% of a disintegrating agent, 10% of a binder, 10% of other auxiliary agents and a second carrier, wherein the balance is made up; the other auxiliary agents comprise a combination of a second wetting agent and a second dispersing agent.

In some embodiments, the weight ratio of the second wetting agent to the second dispersant is 1: (0.5-2.5); preferably, the weight ratio of the second wetting agent to the second dispersing agent is 1: (1-2); more preferably, the weight ratio of the second wetting agent to the second dispersing agent is 1: 1.5.

In some embodiments, the disintegrant is selected from at least one of ammonium sulfate, sodium chloride, urea, starch; preferably, the disintegrant is starch.

In some embodiments, the binder comprises at least one of polyacrylamide, starch, dextrin, gum arabic, soy protein, zein, bone glue, gelatin, sodium silicate, shellac, polyvinyl acetate, polyvinyl alcohol, sodium polyacrylate; preferably, the binder is polyacrylamide.

In some embodiments, the polyacrylamide is a cationic polyacrylamide.

The kind of the cationic polyacrylamide in the invention is not specially limited; preferably, at least one of CP20, CP30, CP40 and CP50 is purchased from texas, h.

In the invention, the inventor finds that when the assistant of the water dispersible granule compounded by diatomite and indoxacarb with a sawtooth-shaped honeycomb structure is ammonium lignosulfonate, a polycarboxylic acid dispersant and sodium dibutylnaphthalenesulfonate, cationic polyacrylamide is selected as a binder, so that the unexpected technical effect is achieved, and the insecticidal efficiency of the system can be further improved. The inventor believes that the polycarboxylic acid dispersant is an anionic high molecular surfactant with a comb-shaped structure, and the cationic polyacrylamide is a cationic high molecular structure, so that when the polycarboxylic acid dispersant is dissolved in water, a certain acting force can be formed between two high molecular polymers with opposite charges, but the polycarboxylate has large space resistance, does not form agglomeration with the cationic polyamide, can maintain a relatively balanced dispersion state, can form enough adsorption force in crops during application, and is used for durably supporting the sawtooth-shaped diatomite and the original drug molecules, thereby improving the efficiency of deinsectization.

A preparation method of water dispersible granules containing the insecticidal and acaricidal composition at least comprises the following steps:

weighing the components, crushing the auxiliary agents to enable the auxiliary agents to be mixed with each other, adding the raw materials, stirring and mixing again, adding the sawtooth-shaped diatomite, sealing, shaking and mixing, transferring into a container, stirring and fully mixing by using a stirrer, and then granulating and drying on a granulator to obtain the composite material.

In the invention, according to the design formula, proper auxiliary agents are weighed and are subjected to high-speed universal grinding, so that the auxiliary agents can be uniformly mixed with each other, and the fineness is reduced. The specific structure diatomaceous earth is then added without mechanical crushing to avoid destroying the structure of the specific structure diatomaceous earth.

The fourth aspect of the invention provides an application of the insecticidal and acaricidal composition, which is applied to control at least one of red spiders, frankliniella occidentalis, trialeurodes vaporariorum, liriomyza, prodenia litura, lesser leafhopper, yellow tea thrips, tea geometrid, lygus lucorum and grape aphids.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

The diatomite with a saw-toothed honeycomb structure described in the following examples is diatomite with a specific structure of Celite610, called CE for short, from Yirui Stone, France: a is the weight ratio.

Examples 1 to 1

Co-toxicity coefficient (CTC) screening: the combined toxicity of the CE and clofentezine on citrus red spiders is determined, and the result is shown in table 1-1.

Referring to the method in NY/T1154.13-2008 standard, a leaf spray method was used. Cleaning and wiping the smooth citrus leaves with the same size, sticking the citrus leaves onto wet sponge with the leaf surfaces facing upwards, enclosing the leaves with wet small cotton slivers to prevent test mites from escaping, putting the wet sponge into a culture dish with the diameter of 9cm, adding water into the culture dish, and slightly lowering the water level to be lower than the height of the sponge. Inoculating indoor cultured nymphs to leaves, each leaf having no less than 30 heads. Placing the culture dish on a bottom plate of a Potter spraying tower for spraying, wherein the spraying amount is 1mL, taking out the culture dish after the liquid medicine is settled for 1min, and transferring the culture dish to a breeding condition for breeding. Each treatment was repeated 4 times and a blank control was run.

After 48h, the death of the test insects was examined under a dissecting mirror and recorded. The judgment standard of the death of the test insects is as follows: death was counted as no response when the test insects were gently touched by tweezers.

The co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 indicates an antagonistic effect, and between 80 and 120 indicates an additive effect. The co-toxicity coefficient is calculated as follows

The Theoretical Toxicity Index (TTI) of the mixture is the toxicity index of the agent A x the percent (%) of the agent A + the toxicity index of the agent B x the percent (%) of the agent B

TABLE 1-1 toxicity test results of CE and clofentezine mixed to citrus red spider

Example 2-1

Co-toxicity coefficient (CTC) screening: the combined toxicity test of CE and indoxacarb on Frankliniella occidentalis, the result is shown in Table 2-1.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf-soaking dish method is adopted. Beating the blades into leaf disks by a puncher with the diameter of 18mm, soaking the leaf disks in a test reagent solution for 10 seconds, putting the leaf disks into a culture dish (with the diameter of 90mm) padded with moisturizing filter paper, putting 5 leaf disks into each dish, naturally drying, then selecting the western flower thrips into the culture dish, sealing the mouth of the dish by a preservative film, pricking some small holes on the film by a needle for ventilation, repeating the treatment for 4 times, finishing the treatment, and placing the dish in an observation room for culture.

And (4) after 72h, checking and recording the death condition of the test insects, wherein the death judgment standard of the test insects is that the test insects are dead as the result of no response when the pointed writing brush is lightly contacted with the test insects.

TABLE 2-1 toxicity test results of CE and indoxacarb mixed on Frankliniella occidentalis

Examples 2 to 2

Co-toxicity coefficient (CTC) screening: the combined toxicity of the CE and the indoxacarb on the trialeurodes vaporariorum is determined, and the result is shown in the table 2-2.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. After dissolving agar (1%) by heating, 20mL to 200mL of the solution was taken out of a beaker and condensed at room temperature for use. Soaking the leaves in a test solution for 10 seconds, taking out, naturally drying, sticking the leaves on the surface of the solidified agar solution, wrapping petioles with wet cotton balls, putting 3-4 leaves in each beaker, inoculating adult trialeurodes vaporariorum, covering 15 heads of each beaker with a preservative film, pricking small holes on the film with needles for ventilation, repeating the treatment for 4 times, and placing the beaker in an observation room for cultivation after the treatment.

And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the tweezers lightly contact the test insects.

TABLE 2-2. toxicity test results of CE and indoxacarb mixed on Bemisia alba

Examples 2 to 3

Co-toxicity coefficient (CTC) screening: the results of the combined virulence determination of the CE and indoxacarb against liriomyza sativae are shown in tables 2-3.

Referring to the method in NY/T1154.13-2008 standard, spraying method is adopted. Transplanting potted cowpea pest-free seedlings into pest-raising cages, inoculating a certain amount of mated adult liriomyza huidonii, taking out bean seedlings with liriomyza huidonii eggs after 6h, placing the bean seedlings in another pest-raising cage, continuously raising the bean seedlings to 2-year larvae (ensuring that each disc contains no less than 80 larvae of 2-year larvae), then carrying out spraying treatment, naturally air drying, and transferring the bean seedlings to an artificial climate box for continuous raising. Each treatment was repeated 4 times and a blank control was run.

And (3) cutting bean seedlings from roots at the later stage of 3-year-old larvae, moisturizing the tail ends by using soaked cotton balls, putting the bean seedlings into plastic basins in an artificial climate box, pupating the bean seedlings, and counting the quantity of pupae in each dish.

TABLE 2-3 toxicity test results of the combination of CE and indoxacarb against Liriomyza sativae

Examples 2 to 4

Co-toxicity coefficient (CTC) screening: the combined toxicity of the CE and indoxacarb on prodenia litura is determined, and the results are shown in tables 2-4.

Referring to the method in NY/T1154.14-2008 standard, the leaf-dipping dish method is adopted. Beat the cabbage leaf into the leaf dish with the hole puncher of diameter 18mm, dip the leaf dish in the liquid of testing medicine for 10 seconds after, put into the culture dish (diameter 90mm) that has filled with the filter paper that moisturizes, 6 leaf dishes are put into to every dish, wait to dry the back naturally, select again and starve 4h twill noctuid 3 age larva to the culture dish in, every dish is not less than 10, 4 times are repeated, the processing finishes, covers the ware lid, arranges the indoor culture of observation in.

TABLE 2-4 toxicity test results of CE and indoxacarb mixed to prodenia litura

Examples 2 to 5

Co-toxicity coefficient (CTC) screening: the combined toxicity of the CE and indoxacarb compounded on the tea leafhoppers is determined, and the results are shown in tables 2-5.

Referring to the method in NY/T1154.11-2008 standard, the dipping method is adopted. Selecting 3 fresh tea tree sprouts, soaking in a test liquid for 30 seconds, taking out, naturally drying, wrapping the base parts of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) with 4 times of repetition per concentration. Then, 3-year-old nymphs of tea lesser leafhoppers subjected to ether mild anesthesia treatment are inoculated into a brush pen, 15 +/-2 heads of each tube are used, and the mouth of each tube is sealed by white gauze. After the treatment, the sample is placed in an observation chamber.

TABLE 2-5 toxicity test results of CE and indoxacarb mixed on tea leaf lesser leafhopper

Examples 2 to 6

Co-toxicity coefficient (CTC) screening: the combined toxicity of the CE and indoxacarb compounded on the tea leaf thrips fulva is determined, and the results are shown in tables 2-6.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf dipping method is adopted. Selecting fresh 3 tea tree sprout shoots with a certain amount of tea thrips (no less than 20) and soaking in the test liquid medicine for 10 seconds, taking out and naturally drying, wrapping the base parts of the sprout shoots with wet absorbent cotton to preserve moisture, sealing the absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) for 4 times of repetition at each concentration. The mouth of the test tube is sealed by a preservative film, and the small holes are punched for ventilation. After the treatment, the sample is placed in an observation chamber.

And after 72h, examining the death condition of the test insects under a dissecting mirror, and recording, wherein the death judgment standard of the test insects is that the test insects die because the test insects are not reacted when the pointed writing brush is lightly contacted with the test insects.

TABLE 2-6 toxicity test results of CE and indoxacarb mixed with tea leaf Arthrips tabaci

Examples 2 to 7

Co-toxicity coefficient (CTC) screening: the combined toxicity of the CE and the indoxacarb compounded on tea geometrid is determined, and the results are shown in tables 2 to 7.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. Selecting 3 fresh tea tree sprouts, soaking in the test liquid medicine for 30 seconds, taking out, naturally drying, wrapping the bases of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a can bottle, wherein the concentration is repeated for 4 times. Then inoculating 2-year-old nymphs of tea geometrid with consistent size and liveness and health into a brush pen, wherein 15 +/-2 heads of each pot are sealed by white gauze. After the treatment, the sample is placed in an observation chamber.

TABLE 2-7 toxicity test results of CE and indoxacarb mixed for tea leaf geometrid

Examples 2 to 8

Co-toxicity coefficient (CTC) screening: the results of the combined virulence determination of CE and indoxacarb paired green plant bug are shown in tables 2-8.

Referring to the method in NY/T1154.11-2008 standard, a leaf spray method was used. The method comprises the steps of disinfecting, cleaning and airing fresh kidney beans by adopting an immersion method, cutting the kidney beans into 2-3 cm segments, immersing the kidney beans in liquid medicines with different concentrations for 30 seconds, airing the kidney beans under natural conditions, and then putting the kidney beans into transparent plastic boxes (6 cm in diameter and 7cm in height) with smooth air, wherein each box has two sections. Healthy and consistent 3-year-old nymphs of lygus lucorum were inoculated, 15 or so per box, repeated 4 times, and a blank control was set. And putting the plastic box into a light incubator for culturing.

And after 48h, the death condition of the test insects in each box is checked, the bodies of the lygus lucorum are lightly touched by the brush tips, and the death judgment standard of the test insects is that no response is calculated when the tweezers lightly touch the test insects.

TABLE 2-8 toxicity test results of CE and indoxacarb mixed with green plant bug

Examples 2 to 9

Co-toxicity coefficient (CTC) screening: the results of the combined toxicity test of CE and indoxacarb on grape aphid are shown in tables 2-9.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Cutting leaves with a certain amount of grape aphids, soaking in the liquid medicine to be tested for 10 seconds, taking out, absorbing the liquid medicine around the insect body with absorbent filter paper, placing in a culture dish padded with moisturizing filter paper, moisturizing with a wet cotton ball, and covering the culture dish with wet gauze. Each 30 aphids were treated, 4 replicates and a blank treatment without drug (including solvent and emulsifier used) was used. After the treatment, the cells were transferred to an observation room for cultivation.

And (3) detecting the death condition of the test insects after 48h, and recording, wherein the judgment standard of the death of the test insects is as follows: the aphids were lightly touched with a writing brush, and the body was judged dead.

TABLE 2-9 toxicity test results of CE and indoxacarb mixed on grape aphid

Example 3-1

An insecticidal and acaricidal composition with high efficiency comprises raw materials of diatomite with a saw-tooth honeycomb structure and a raw pesticide, wherein the raw pesticide is clofentezine; the weight ratio of the diatomite and the clofentezine with the sawtooth-shaped honeycomb structure is 50: 1.

the wettable dispersant containing the insecticidal and acaricidal composition comprises the following raw materials in percentage by weight: 50% of the insecticidal and acaricidal composition, 7% of an auxiliary agent and a first carrier to make up the balance; the adjuvant comprises a combination of a first wetting agent and a first dispersant. The weight ratio of the first wetting agent to the first dispersing agent is 1: 1.5.

the first wetting agent is selected from sodium dibutylnaphthalenesulfonate.

The first dispersant is a combination of ammonium lignosulfonate and a polycarboxylic acid dispersant; the weight ratio of the ammonium lignosulfonate to the polycarboxylic acid dispersant is 0.3: 1. the polycarboxylic acid dispersant is polycarboxylate dispersant GY-D06.

The first carrier is kaolin. The mesh number of the kaolin is 1500 meshes.

Example 4-1

An efficient insecticidal and acaricidal composition comprises raw materials of diatomite with a saw-tooth honeycomb structure and a raw pesticide, wherein the raw pesticide is indoxacarb; the weight ratio of the diatomite and the indoxacarb with the sawtooth-shaped honeycomb structure is 200: 1.

the first wetting agent is selected from sodium dibutylnaphthalenesulfonate.

The first carrier is kaolin. The mesh number of the kaolin is 1500 meshes.

Example 4 to 2

An efficient insecticidal and acaricidal composition comprises raw materials of diatomite with a saw-tooth honeycomb structure and a raw pesticide, wherein the raw pesticide is indoxacarb; the weight ratio of the diatomite and the indoxacarb with the sawtooth-shaped honeycomb structure is 1: 1.

the water dispersible granule containing the insecticidal and acaricidal composition at least comprises the following raw materials in percentage by weight: 45% of the insecticidal and acaricidal composition, 12% of a disintegrating agent, 10% of a binder, 10% of other auxiliary agents and a second carrier, wherein the balance is made up; the other auxiliary agents comprise a combination of a second wetting agent and a second dispersing agent. The weight ratio of the second wetting agent to the second dispersing agent is 1: 1.5.

the disintegrating agent is starch.

The binder is polyacrylamide. The polyacrylamide is cationic polyacrylamide. Purchased from texas, henna, water treatment materials, inc, CP 50.

The second wetting agent is selected from sodium dibutylnaphthalenesulfonate.

The second dispersant is the combination of ammonium lignosulfonate and polycarboxylic acid dispersant; the weight ratio of the ammonium lignosulfonate to the polycarboxylic acid dispersant is 0.3: 1.

the polycarboxylic acid dispersant is polycarboxylate dispersant GY-D06.

The second carrier is kaolin, and the mesh number of the kaolin is 1500 meshes.

Comparative example 1

The difference from the example 3-1 is that the first dispersant is ammonium lignosulfonate, aralkyl phenol polyoxyethylene ether phosphate; the weight ratio of the ammonium lignosulphonate to the aralkyl phenol polyoxyethylene ether phosphate is 0.3: 1.

comparative example 2

The difference from example 3-1 is that the first wetting agent is selected from sodium alpha-alkenyl sulfonate.

Comparative example 3

The difference from the embodiment 3-1 is that the weight ratio of the first wetting agent to the first dispersing agent is 5: 1.

performance testing

1. Measurement of suspension percentage: the suspension percentage of the wettable dispersant containing the insecticidal and acaricidal composition is determined according to the method GB/T14825-2006, and the result is shown in Table 3.

The method comprises the following steps: the sample to be tested is prepared into a suspension of appropriate concentration with standard hard water. And (3) standing in a measuring cylinder for a certain time under a specified condition, measuring the mass fraction of the effective components in the suspension at one tenth of the bottom, and calculating the suspension rate.

TABLE 3 results of suspension percentage measurement

Examples	Suspension Rate (%)
		Example 3-1	93.4
Example 4-1	91.6
		Comparative example 1	82.5
Comparative example 2	86.2
		Comparative example 3	88.3

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art may modify or change the technical content disclosed above into an equivalent embodiment with equivalent changes, but all those simple modifications, equivalent changes and modifications made on the above embodiment according to the technical spirit of the present invention still belong to the protection scope of the present invention.

Claims

1. An efficient insecticidal and acaricidal composition is characterized in that raw materials comprise diatomite with a saw-tooth honeycomb structure and raw pesticide in a weight ratio of (1: 40) - (400: 1); the technical material is at least one of clofentezine and indoxacarb.

2. An insecticidal and acaricidal composition according to claim 1 wherein said bulk drug is clofentezine; the weight ratio of the diatomite and the clofentezine in the sawtooth-shaped honeycomb structure is (1: 40) - (100: 1).

3. An insecticidal and acaricidal composition according to claim 1 or 2, wherein the application properties are selected from one of emulsifiable concentrate, suspending agent, wettable powder, water dispersible granule and aqueous agent.

4. A wettable dispersant comprising a pesticidal and acaricidal composition according to claim 1 or 2, characterized in that the raw materials comprise, in weight percent, at least: 10-80% of the insecticidal and acaricidal composition, 5-10% of an auxiliary agent and a first carrier to make up the balance; the auxiliary agent comprises at least one of a first wetting agent and a first dispersing agent.

5. A wettable dispersant for an insecticidal and acaricidal composition according to claim 4 wherein said first wetting agent and said first dispersant are present in a weight ratio of 1: (0.5-2.5); the first wetting agent is selected from at least one of sodium dibutylnaphthalene sulfonate, sodium diisopropyl naphthalene sulfonate, alkyl naphthalene sulfonate, sodium dodecyl benzene sulfonate and sodium dodecyl sulfate; the first dispersant is at least one selected from ammonium lignosulfonate, polycarboxylic acid dispersant, NNO, MF, CNF, EO/PO block polyether and aralkyl phenol polyoxyethylene ether phosphate.

6. A wettable dispersant for an insecticidal and acaricidal composition according to claim 5 wherein said first dispersant is a combination of ammonium lignosulfonate, a polycarboxylic acid dispersant; the weight ratio of the ammonium lignosulphonate to the polycarboxylic acid dispersant is (0.1-0.5): 1.

7. a water dispersible granule comprising the insecticidal and acaricidal composition according to claim 1 or 2, characterized in that the raw materials comprise, in weight percent, at least: 5-60% of the insecticidal and acaricidal composition, 0.5-20% of a disintegrating agent, 5-15% of a binder, 5-15% of other additives and a second carrier for balancing; the other auxiliary agent comprises at least one of a second wetting agent and a second dispersing agent.

8. The insecticidal and acaricidal composition in accordance with claim 7 wherein said binder comprises at least one of polyacrylamide, starch, dextrin, gum arabic, soy protein, zein, bone glue, gelatin, sodium silicate, shellac, polyvinyl acetate, polyvinyl alcohol, sodium polyacrylate.

9. A water dispersible granule of an insecticidal and acaricidal composition according to claim 8, wherein said polyacrylamide is a cationic polyacrylamide.

10. An insecticidal and acaricidal composition according to claim 1 or 2, which is used for controlling at least one of red spider, frankliniella occidentalis, trialeurodes vaporariorum, liriomyza sativae, prodenia litura, lesser leafhopper, thrips theacea, ectropis obliqua, lygus lucorum and grape aphid.