CN111213641A

CN111213641A - Insecticidal composition containing diatomite and alkaloid

Info

Publication number: CN111213641A
Application number: CN202010100821.8A
Authority: CN
Inventors: 陈瑞明
Original assignee: Benson Huizhou Biotechnology Co Ltd
Current assignee: Benson Huizhou Biotechnology Co Ltd
Priority date: 2020-02-19
Filing date: 2020-02-19
Publication date: 2020-06-02

Abstract

The application belongs to the technical field of pesticides, and particularly relates to an insecticidal composition containing diatomite and alkaloid. The invention discloses an insecticidal composition containing diatomite and alkaloid, which contains diatomite and alkaloid with specific structures, wherein the mass ratio of the diatomite and the alkaloid with the specific structures is (1:60) - (400:1), the diatomite with the specific structures is diatomite with a saw-tooth honeycomb structure, and the alkaloid is matrine or veratrine.

Description

Insecticidal composition containing diatomite and alkaloid

Technical Field

The application belongs to the technical field of pesticides, and particularly relates to an insecticidal composition containing diatomite and alkaloid.

Background

Matrine is prepared from dried root, plant and fruit of Sophora flavescens ait of Leguminosae by extracting with organic solvent such as ethanol, and is an alkaloid. Matrine pesticide used in agriculture means practically all substances extracted from sophora flavescens, such as a begonia extract or total matrines, and is mainly used for controlling various pests such as pine moth, tea moth, cabbage caterpillar and the like. Has multiple functions of insecticidal activity, bactericidal activity, plant growth regulating function and the like. The matrine as the biological pesticide has the characteristics that: the matrine is a botanical pesticide, has the characteristics of specificity and naturalness, only has an effect on specific organisms, can be quickly decomposed in nature, and the final products are carbon dioxide and water. And secondly, the matrine is a plant endogenous chemical substance with activity on pests, and the components are not single, but are combined by a plurality of groups with similar chemical structures and a plurality of groups with dissimilar chemical structures, supplement each other and play a role together. Thirdly, the matrine is not easy to cause the harmful substances to generate drug resistance due to the combined action of a plurality of chemical substances, and can be used for a long time. Fourth, the corresponding pest is not directly and completely poisoned, but the production and multiplication of the plant population are not seriously influenced by controlling the number of pest biological populations.

The main chemical components of veratrine are thatadine and veratridine. Flat needle-like crystals. The veratrum alkaloid is present in plants of genus Veratrum and genus sneeze of family Liliaceae, and the plant material used as pesticide mainly comprises seed of the plant of genus Veratrum and rhizome of resveratrol. Extracting the plant material with ethanol. The botanical pesticide has contact poisoning and stomach poisoning effects on insects. Can be used for preventing and treating sanitary pests such as housefly, cockroach, louse and the like, and can also be used for preventing and treating agricultural pests such as cabbage caterpillar, aphid, leafhopper, thrips, stinkbug and the like. The main insecticidal action mechanism of the pesticide is that after entering a digestive system through the epidermis or the suction of a polypide, the pesticide causes local stimulation to cause reflex polypide excitation, firstly inhibits sensory nerve endings of the polypide, and then inhibits central nerves to cause death of pests. Veratrine has low toxicity to human and livestock, low residue, no environmental pollution, and long-lasting drug effect (more than 10 days), and is effective in preventing and treating vegetable pests.

However, matrine and veratrine are specific in biological range, so that the field of prevention and treatment is limited while the matrine and veratrine have specific effects, and the matrine and the veratrine are usually required to be matched with other pesticide raw materials for use so as to improve the antibacterial spectrum of the pesticide.

Disclosure of Invention

In order to solve the technical problems, the first aspect of the invention provides an insecticidal composition containing diatomite and alkaloid, the composition contains diatomite and alkaloid with specific structures, wherein the mass ratio of the diatomite and the alkaloid with specific structures is (1:60) - (400:1), the diatomite with specific structures is diatomite with a saw-tooth honeycomb structure, and the alkaloid is matrine or veratrine.

As a preferred technical scheme, the mass ratio of the diatomite and the alkaloid with the specific structure is one of (1:60) - (350:1), (1:60) - (300:1), (1:60) - (250:1), (1:60) - (200:1), (1:60) - (150:1), (1:60) - (100:1), (1:60) - (50:1), (1:60) - (1:1), (1:50) - (400:1), (1:40) - (400:1), (1:30) - (400:1), (1:20) - (400:1), (1:10) - (400:1) and (1:1) - (400: 1).

As a preferable technical scheme, the mass ratio of the diatomite with the specific structure to the alkaloid is one of 400:1, 350:1, 300:1, 250:1, 200:1, 150:1, 100:1, 50:1, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50 and 1: 60.

As a preferable technical scheme, the composition is prepared into wettable powder, water dispersible granules, aqueous emulsion, soluble powder, suspending agent or granules.

As a preferable technical scheme, the insecticidal composition is a wettable powder containing the following components in percentage by mass: 50-90% of diatomite and alkaloid with specific structures and 10-50% of auxiliary agent.

As a preferred technical scheme, the auxiliary agent comprises a copolymer of methacrylic acid and polyoxyethylene.

As a preferred embodiment, the methacrylic includes methacrylic acid and/or methyl methacrylate.

As a preferable technical scheme, the auxiliary agent also comprises alkyl naphthalene sulfonate.

The second aspect of the invention provides a preparation method of the insecticidal composition, which comprises the following steps: according to the design formula, the auxiliary agent, the alkaloid and the diatomite with the specific structure are stirred and mixed uniformly to obtain the product.

The third aspect of the invention provides application of the insecticidal composition in prevention and control of citrus red spider, scale insects, frankliniella occidentalis, whitefly, liriomyza sativae, prodenia litura, tea leafhopper, tea yellow thrips, tea looper, lygus lucorum and grape aphid.

Has the advantages that: the insecticidal composition has control effects on citrus red spiders, citrus scale insects, frankliniella occidentalis, trialeurodes vaporariorum, liriomyza sativae, prodenia litura, tea leafhopper, tea yellow thrips, tea geometrid, green plant bugs and grape aphids, and the insecticidal range is expanded; the insecticidal effect is improved; the environmental pollution is reduced.

Drawings

FIG. 1 is an optical microscope of diatomaceous earth having a particular structure of a saw-tooth honeycomb structure according to the present invention.

Detailed Description

In order to solve the problems, the invention provides an insecticidal composition containing diatomite and alkaloid, which contains diatomite and alkaloid with specific structures, wherein the mass ratio of the diatomite and the alkaloid with the specific structures is (1:60) - (400:1), the diatomite with the specific structures is diatomite with a saw-tooth honeycomb structure, and the alkaloid is matrine or veratrine.

In the present invention, the term "composition" means that the components of the composition are packaged in combination or separately and used together when used. That is, in the present invention, the phrase "containing diatomaceous earth and alkaloid" means that diatomaceous earth and alkaloid can be mixed and packaged together and used together when used; also comprises the steps of separately packaging the diatomite and the alkaloid and mixing the diatomite and the alkaloid together according to a certain proportion when in use.

The proportion of the diatomite and the alkaloid with specific structures in the composition can be adjusted within a reasonable range according to specific application conditions. For example, the mass ratio of the diatomite and the matrine with the specific structure is one of (1:60) - (350:1), (1:60) - (300:1), (1:60) - (250:1), (1:60) - (200:1), (1:60) - (150:1), (1:60) - (100:1), (1:60) - (50:1), (1:60) - (1:1), (1:50) - (400:1), (1:40) - (400:1), (1:30) - (400:1), (1:20) - (400:1), (1:10) - (400:1) and (1:1) - (400: 1); in some preferred embodiments, the ratio of diatomaceous earth to matrine of a particular structure is one of 400:1, 350:1, 300:1, 250:1, 200:1, 150:1, 100:1, 50:1, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, 1:60 by mass.

For example, the mass ratio of the diatomite with a specific structure to the veratrine is one of (1:60) - (350:1), (1:60) - (300:1), (1:60) - (250:1), (1:60) - (200:1), (1:60) - (150:1), (1:60) - (100:1), (1:60) - (50:1), (1:60) - (1:1), (1:50) - (400:1), (1:40) - (400:1), (1:30) - (400:1), (1:20) - (400:1), (1:10) - (400:1) and (1:1) - (400: 1); in some preferred embodiments, the ratio of diatomaceous earth to veratrine of a particular structure is one of 400:1, 350:1, 300:1, 250:1, 200:1, 150:1, 100:1, 50:1, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, 1:60, by mass.

In a preferred embodiment, the diatomaceous earth of the specific structure is Celite610 diatomaceous earth of Yirui Stone, France. As shown in figure 1, the diatomite has a special sawtooth honeycomb structure, is a physical insecticide, and can be adsorbed on a wax layer on the surface of an insect body on one hand, so that the insect is dehydrated and killed; on the other hand, the diatomite with the sawtooth structure can damage a wax layer on the body surface of the insect and tear the body surface of the insect, and can be used for preventing and controlling beetles, tobacco hornworms, diaphorina citri and the like.

Matrine is a plant endogenous chemical substance with activity on pests, the components are not single, but the combination of a plurality of groups with similar chemical structures and a plurality of groups with dissimilar chemical structures, the insecticidal mechanism of matrine is probably to act on the nervous system, firstly paralyze the central nerve, then excite the central nerve, further act on the diaphragm and the respiratory muscle nerve, and suffocate and die insects.

The main insecticidal action mechanism of veratrine is that after entering the digestive system through the epidermis or the suction of the polypide, local stimulation is caused to arouse the reflex polypide, the sensory nerve endings of the polypide are inhibited firstly, and then the central nerve is inhibited to cause the death of pests.

According to the application, the diatomite with the sawtooth honeycomb structure and the alkaloid are compounded in a specific proportion, so that matrine or veratrine is promoted to enter a pest body, and acts on a pest nervous system or causes local stimulation to cause reflex pest body excitation or central nerve excitation to cause pest death, the pest killing range is expanded, respective defects are effectively overcome, respective drug effects are promoted to be exerted, and good control effects are achieved on citrus red spiders, citrus scale insects, frankliniella occidentalis, aleyrodids leucadendra, lirioides pulvinata, prodenia litura, tea leafhopper, tea leaf tea thrips, tea leaf tea geometrid moth, green plant bug and grape aphid.

The composition can be prepared into wettable powder, water dispersible granules, aqueous emulsion, soluble powder, suspending agent or granules.

As a preferred embodiment, the composition of the present invention is a wettable powder containing the following components in mass content: 50-90% of diatomite and alkaloid with specific structures and 10-50% of auxiliary agent.

Preferably, the composition of the invention is a wettable powder containing the following components in percentage by mass: 60-80% of diatomite and alkaloid with specific structures and 10-30% of auxiliary agent.

More preferably, the composition of the invention is a wettable powder containing the following components in percentage by mass: diatomite with a specific structure, 70% of alkaloid and 30% of auxiliary agent.

The wettable powder of the present invention is used by spraying with water, and the suspension rate at the time of use is an important factor affecting the efficacy of the wettable powder.

In some embodiments, the adjuvant comprises a copolymer of methacrylic acid and polyoxyethylene; preferably, the methacrylic comprises methacrylic acid and/or methyl methacrylate; preferably, the average molecular weight of the dispersant is 20000-30000; the dispersant is a commercial product, and may be exemplified by Atlox 4913, british grass.

In some embodiments, the adjuvant further comprises an alkyl naphthalene sulfonate; examples of the alkylnaphthalene sulfonate include butylnaphthalene sulfonate, methylnaphthalene sulfonate, and propylnaphthalene sulfonate; preferably, the alkyl naphthalene sulfonate is nekal BX.

The copolymer of methacrylic acid and polyoxyethylene takes a methacrylic acid-methyl methacrylate copolymer chain segment as an oleophylic group, is firmly adsorbed on diatomite with a special sawtooth honeycomb structure, cannot be desorbed due to shaking and the like, and meanwhile, the polyoxyethylene chain segment with proper length is arranged around particles, so that strong acting force can be generated with a water system to further prevent aggregation among the particles; the addition of alkylnaphthalene sulfonate, especially butylnaphthalene sulfonate nekal BX, can promote the interaction between the copolymer of methacrylic acid and polyoxyethylene and the particles, and the alkyl group on the butylnaphthalene sulfonate can be entangled with the methacrylic acid-methyl methacrylate copolymer chain segment, thereby further improving the dispersion stability.

Preferably, the auxiliary agent further comprises white carbon black; the type of the white carbon black is not particularly limited, and the white carbon black is mainly used as an adsorbent.

Preferably, the methacrylic acid-polyoxyethylene copolymer: alkyl naphthalene sulfonate: the mass ratio of the white carbon black is (4-6): 7: 3.

more preferably, the methacrylic acid-polyoxyethylene copolymer: alkyl naphthalene sulfonate: the mass ratio of the white carbon black is 5: 7: 3.

in a more preferred embodiment, the specific structure of diatomaceous earth and alkaloid has a mass ratio of 200:1, the mass ratio of the methacrylic acid-polyoxyethylene copolymer to the alkyl naphthalene sulfonate is (4-6): 7.

In a more preferred embodiment, the specific structure of diatomaceous earth and alkaloid has a mass ratio of 200:1, the mass ratio of the copolymer of methacrylic acid and polyoxyethylene to the alkyl naphthalene sulfonate is 5: 7.

The applicant finds that under the premise of determining the mass content of the alkaloid and the diatomite with a specific structure in the wettable powder, the alkyl naphthalene sulfonate: when the proportion of the copolymer of methacrylic acid and polyoxyethylene is reduced, the wetting time of the wettable powder is reduced no matter how the proportion of the alkaloid and the diatomite with a specific structure is changed; with the increase of the content of alkaloid, the wetting time is increased, then reduced and then increased; the applicant finds that wetting is easier due to the special structure of the diatomite, the wetting time can be reduced by increasing the content of the diatomite, but when the content of the diatomite is too much, agglomeration can occur between the diatomite, and the wetting performance is influenced, when the diatomite is in an intermediate concentration, the wetting condition becomes very complicated due to the action of alkyl naphthalene sulfonate, methacrylic acid and polyoxyethylene copolymer and alkyl naphthalene sulfonate, and the applicant finds that when the mass ratio of the diatomite with a specific structure to the alkaloid is 200: when the mass ratio of the methacrylic acid-polyoxyethylene copolymer to the alkylnaphthalene sulfonate is (4-6):7, the wetting time is unexpectedly reduced.

The preparation method of the wettable powder comprises the following steps: according to the design formula, the auxiliary agent, the alkaloid and the diatomite with the specific structure are stirred and mixed uniformly to obtain the product.

When the used auxiliary agent contains liquid, the white carbon black is put into a beaker, the liquid auxiliary agent is added while stirring, and after the white carbon black is completely adsorbed, high-speed universal grinding is carried out, so that the white carbon black and the liquid auxiliary agent can be uniformly mixed with each other, and the fineness is reduced.

Preferably, the preparation method of the wettable powder comprises the following steps: according to the design formula, the white carbon black is put into a beaker, the liquid auxiliary agent is added while stirring, and after the white carbon black is completely adsorbed, the white carbon black is subjected to high-speed universal grinding with the alkaloid and the other auxiliary agents, so that the white carbon black and the alkaloid can be uniformly mixed. Then adding diatomite with a specific structure, shaking by using a self-sealing bag, transferring into a flask after 5-15min, stirring and mixing for 30min by using a stirrer, and fully and uniformly mixing.

In this application, mechanical crushing is not used in order to avoid destroying the diatomaceous earth structure of a particular structure.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

In addition, the starting materials used are all commercially available, unless otherwise specified.

Examples

Examples 1-11 are combined virulence assays for Celite610 and matrine combinations.

Reagent to be tested: matrine is 0.3% wettable powder (purchased from Yale Biotech GmbH, Henan province, Ltd.), Celite610 diatomaceous earth;

the co-toxicity coefficient (CTC) of each mixed combination is determined by a Sun & Johnson (1960) co-toxicity coefficient method (CTC) according to a raw test standard method NY/T1154.7-2006. Generally, a co-toxicity coefficient greater than 120 indicates a synergistic effect, less than 80 indicates an antagonistic effect, and between 80 and 120 indicates an additive effect. The co-toxicity coefficient is calculated as follows

The Theoretical Toxicity Index (TTI) of the mixture is the toxicity index of the agent A x the percent (%) of the agent A + the toxicity index of the agent B x the percent (%) of the agent B

Example 1, co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and matrine on citrus red spiders.

Referring to the method in NY/T1154.13-2008 standard, a leaf spray method was used. Cleaning and wiping the smooth citrus leaves with the same size, sticking the citrus leaves onto wet sponge with the leaf surfaces facing upwards, enclosing the leaves with wet small cotton slivers to prevent test mites from escaping, putting the wet sponge into a culture dish with the diameter of 9cm, adding water into the culture dish, and slightly lowering the water level to be lower than the height of the sponge. Inoculating indoor cultured nymphs to leaves, each leaf having no less than 30 heads. Placing the culture dish on a bottom plate of a Potter spraying tower for spraying, wherein the spraying amount is 1mL, taking out the culture dish after the liquid medicine is settled for 1min, and transferring the culture dish to a breeding condition for breeding. Each treatment was repeated 4 times and a blank control was run.

After 48h, the death of the test insects was examined under a dissecting mirror and recorded. The judgment standard of the death of the test insects is as follows: death was counted as no response when the test insects were gently touched by tweezers.

TABLE 1 toxicity test results of blending Celite610 and matrine to citrus red spider

Example 2 co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and matrine on the scale insects of citrus.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Dipping the trimmed citrus leaves with citrus arrowhead scales in a test solution for 10 seconds, taking out the citrus leaves, absorbing redundant liquid medicine around the leaves and the insects by using filter paper, putting the citrus leaves and the insects into a culture dish (with the diameter of 90mm) filled with moisturizing filter paper, wrapping petioles by using wet cotton balls for moisturizing, repeating the steps for 4 times per concentration, covering the dish cover after the treatment is finished, and placing the dish cover in an observation room for culturing.

After 72d, checking the death condition of the test insects by using a dissecting mirror, and recording, wherein the death judgment standard of the test insects is fresh and alive, and the test insects are regarded as live insects in yellow or light yellow; the dead insects are considered to be dead insects if the body of the insects is shriveled and the color is dark.

TABLE 2 toxicity test results of Celite610 and matrine mixed to Seicerus citricola (Lecanicillium)

Example 3 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and matrine on Frankliniella occidentalis.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf-soaking dish method is adopted. Beating the blades into leaf disks by a puncher with the diameter of 18mm, soaking the leaf disks in a test reagent solution for 10 seconds, putting the leaf disks into a culture dish (with the diameter of 90mm) padded with moisturizing filter paper, putting 5 leaf disks into each dish, naturally drying, then selecting the western flower thrips into the culture dish, sealing the mouth of the dish by a preservative film, pricking some small holes on the film by a needle for ventilation, repeating the treatment for 4 times, finishing the treatment, and placing the dish in an observation room for culture.

And (4) after 72h, checking and recording the death condition of the test insects, wherein the death judgment standard of the test insects is that the test insects are dead as the result of no response when the pointed writing brush is lightly contacted with the test insects.

TABLE 3 toxicity test results of mixing Celite610 and matrine against Frankliniella occidentalis

Example 4 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and matrine on the trialeurodes vaporariorum.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. After dissolving agar (1%) by heating, 20mL to 200mL of the solution was taken out of a beaker and condensed at room temperature for use. Soaking the leaves in a test solution for 10 seconds, taking out, naturally drying, sticking the leaves on the surface of the solidified agar solution, wrapping petioles with wet cotton balls, putting 3-4 leaves in each beaker, inoculating adult trialeurodes vaporariorum, covering 15 heads of each beaker with a preservative film, pricking small holes on the film with needles for ventilation, repeating the treatment for 4 times, and placing the beaker in an observation room for cultivation after the treatment.

And (4) after 72h, detecting the death condition of the test insects, and recording, wherein the death judgment standard of the test insects is that no response is calculated when the tweezers lightly contact the test insects.

TABLE 4 toxicity test results of mixing Celite610 and matrine against Bemisia alba

Example 5 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the compounding of the Celite610 and the matrine to the liriomyza sativae.

Referring to the method in NY/T1154.13-2008 standard, spraying method is adopted. Transplanting potted cowpea pest-free seedlings into pest-raising cages, inoculating a certain amount of mated adult liriomyza huidonii, taking out bean seedlings with liriomyza huidonii eggs after 6h, placing the bean seedlings in another pest-raising cage, continuously raising the bean seedlings to 2-year larvae (ensuring that each disc contains no less than 80 larvae of 2-year larvae), then carrying out spraying treatment, naturally air drying, and transferring the bean seedlings to an artificial climate box for continuous raising. Each treatment was repeated 4 times and a blank control was run.

And (3) cutting bean seedlings from roots at the later stage of 3-year-old larvae, moisturizing the tail ends by using soaked cotton balls, putting the bean seedlings into plastic basins in an artificial climate box, pupating the bean seedlings, and counting the quantity of pupae in each dish.

TABLE 5 toxicity test results of blending Celite610 and matrine against liriomyza sativae

Example 6 co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and matrine on prodenia litura.

Referring to the method in NY/T1154.14-2008 standard, the leaf-dipping dish method is adopted. Beat the cabbage leaf into the leaf dish with the hole puncher of diameter 18mm, dip the leaf dish in the liquid of testing medicine for 10 seconds after, put into the culture dish (diameter 90mm) that has filled with the filter paper that moisturizes, 6 leaf dishes are put into to every dish, wait to dry the back naturally, select again and starve 4h twill noctuid 3 age larva to the culture dish in, every dish is not less than 10, 4 times are repeated, the processing finishes, covers the ware lid, arranges the indoor culture of observation in.

TABLE 6 toxicity test results of mixing Celite610 and matrine against spodoptera litura

Example 7 co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and matrine on the tea leafhopper.

Referring to the method in NY/T1154.11-2008 standard, the dipping method is adopted. Selecting 3 fresh tea tree sprouts, soaking in a test liquid for 30 seconds, taking out, naturally drying, wrapping the base parts of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) with 4 times of repetition per concentration. Then, 3-year-old nymphs of tea lesser leafhoppers subjected to ether mild anesthesia treatment are inoculated into a brush pen, 15 +/-2 heads of each tube are used, and the mouth of each tube is sealed by white gauze. After the treatment, the sample is placed in an observation chamber.

TABLE 7 toxicity test results of mixing Celite610 and matrine on tea leafhoppers

Example eight co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and matrine on the tea leaf thrips tabaci.

According to the standard method NY/T1154.14-2008 of biological measurement, a leaf dipping method is adopted. Selecting fresh 3 tea tree sprout shoots with a certain amount of tea thrips (no less than 20) and soaking in the test liquid medicine for 10 seconds, taking out and naturally drying, wrapping the base parts of the sprout shoots with wet absorbent cotton to preserve moisture, sealing the absorbent cotton balls with a preservative film, and placing in a glass test tube (phi 200 x 30mm) for 4 times of repetition at each concentration. The mouth of the test tube is sealed by a preservative film, and the small holes are punched for ventilation. After the treatment, the sample is placed in an observation chamber.

And after 72h, examining the death condition of the test insects under a dissecting mirror, and recording, wherein the death judgment standard of the test insects is that the test insects die because the test insects are not reacted when the pointed writing brush is lightly contacted with the test insects.

TABLE 8 toxicity test results of mixture of Celite610 and matrine on tea leaf thrips

Example 9 co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and matrine compounded on tea leaf geometrid.

Referring to the method in NY/T1154.14-2008 standard, the leaf soaking method is adopted. Selecting 3 fresh tea tree sprouts, soaking in the test liquid medicine for 30 seconds, taking out, naturally drying, wrapping the bases of the sprouts with wet absorbent cotton to preserve moisture, sealing absorbent cotton balls with a preservative film, and placing in a can bottle, wherein the concentration is repeated for 4 times. Then inoculating 2-year-old nymphs of tea geometrid with consistent size and liveness and health into a brush pen, wherein 15 +/-2 heads of each pot are sealed by white gauze. After the treatment, the sample is placed in an observation chamber.

TABLE 9 toxicity test results of mixing Celite610 and matrine to tea leaf geometrid

Example 10 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the compounding of the Celite610 and the matrine to the green plant bug.

Referring to the method in NY/T1154.11-2008 standard, a leaf spray method was used. The method comprises the steps of disinfecting, cleaning and airing fresh kidney beans by adopting an immersion method, cutting the kidney beans into 2-3 cm segments, immersing the kidney beans in liquid medicines with different concentrations for 30 seconds, airing the kidney beans under natural conditions, and then putting the kidney beans into transparent plastic boxes (6 cm in diameter and 7cm in height) with smooth air, wherein each box has two sections. Healthy and consistent 3-year-old nymphs of lygus lucorum were inoculated, 15 or so per box, repeated 4 times, and a blank control was set. And putting the plastic box into a light incubator for culturing.

And after 48h, the death condition of the test insects in each box is checked, the bodies of the lygus lucorum are lightly touched by the brush tips, and the death judgment standard of the test insects is that no response is calculated when the tweezers lightly touch the test insects.

TABLE 10 toxicity test results of mixing Celite610 and matrine to green plant bug

Example 11, co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and matrine on grape aphids.

The method in NY/T1154.6-2006 is referred to, and the insect-soaking method is adopted. Cutting leaves with a certain amount of grape aphids, soaking in the liquid medicine to be tested for 10 seconds, taking out, absorbing the liquid medicine around the insect body with absorbent filter paper, placing in a culture dish padded with moisturizing filter paper, moisturizing with a wet cotton ball, and covering the culture dish with wet gauze. Each 30 aphids were treated, 4 replicates and a blank treatment without drug (including solvent and emulsifier used) was used. After the treatment, the cells were transferred to an observation room for cultivation.

And (3) detecting the death condition of the test insects after 48h, and recording, wherein the judgment standard of the death of the test insects is as follows: the aphids were lightly touched with a writing brush, and the body was judged dead.

TABLE 11 toxicity test results of mixing Celite610 with matrine against aphid of grape

Examples 12-22 are combined virulence assays for Celite610 formulated with veratrine.

Reagent to be tested: veratrine is 0.5% wettable powder purchased from Daphne foeniculi grass Biotech ltd, Celite610 diatomaceous earth;

Example 12 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and veratrine on citrus red spiders.

After 72h, the death of the test insects was examined under a dissecting mirror and recorded. The judgment standard of the death of the test insects is as follows: death was counted as no response when the test insects were gently touched by tweezers.

TABLE 12 toxicity assay results for citrus red spiders by Celite610 blended with veratrine

Example 13 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and veratrine on the scale insects of citrus.

After 72h, examining the death condition of the test insects by using a dissecting mirror, and recording, wherein the death judgment standard of the test insects is fresh and alive, and the test insects are regarded as live insects in yellow or light yellow; the dead insects are considered to be dead insects if the body of the insects is shriveled and the color is dark.

TABLE 13 toxicity assay results for Citrus scale insect (arrowhead scale) with Celite610 blended with veratrine

Example 14, co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and veratrine on Frankliniella occidentalis.

TABLE 14 toxicity test results of Celite610 and veratrine blending on Frankliniella occidentalis

Example 15 co-toxicity coefficient (CTC) screening: and (3) carrying out combined toxicity determination on the trialeurodes vaporariorum by compounding Celite610 and veratrine.

TABLE 15 toxicity test results of blending Celite610 and veratrine against Bemisia alba

Example 16 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and veratrine on the liriomyza sativae.

TABLE 16 toxicity test results of blending Celite610 and veratrine against Liriomyza sativae

Example 17 co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and veratrine on prodenia litura.

TABLE 17 toxicity test results of blending Celite610 and veratrine against spodoptera litura

Example 18 co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and veratrine compounded on the tea leafhopper.

TABLE 18 toxicity test results of Celite610 and veratrine mixed on tea leafhopper

Example 19 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and veratrine on the tea leaf thrips.

TABLE 19 toxicity test results of mixture of Celite610 and veratrine on tea leaf thrips

Example 20 co-toxicity coefficient (CTC) screening: and (3) determining the combined toxicity of the Celite610 and veratrine compounded on tea leaf geometrid.

TABLE 20 toxicity test results of Celite610 and veratrine mixed for tea leaf geometrid

Example 21 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and veratrine on the green plant bug.

TABLE 21 toxicity assay results of blending of Celite610 and veratrine to apolygus lucorum

Example 22 co-toxicity coefficient (CTC) screening: and (3) testing the combined toxicity of the Celite610 and veratrine on grape aphids.

TABLE 22 toxicity assay results of Celite610 and veratrine blending on grape aphid

Example 23

A wettable powder contains the following components by mass: 70% of Celite610 diatomite and matrine of Yirui stone and 30% of auxiliary agent.

The mass ratio of the Celite610 diatomite to the matrine of the Yirui stone is 200: 1.

the auxiliary agent comprises a copolymer of methacrylic acid and polyoxyethylene, alkyl naphthalene sulfonate and white carbon black; the copolymer of methacrylic acid and polyoxyethylene: alkyl naphthalene sulfonate: the mass ratio of the white carbon black is 5: 7: 3.

the copolymer of methacrylic acid and polyoxyethylene is Atlox 4913, british grass. The alkyl naphthalene sulfonate is nekal BX.

The preparation method of the wettable powder comprises the following steps: according to the design formula, white carbon black is put into a beaker, great British grass Atlox 4913 is added while stirring, and after complete adsorption, the white carbon black is subjected to high-speed universal pulverization with alkaloid and nekal BX to be uniformly mixed with each other. Then adding diatomite with a specific structure, shaking by using a self-sealing bag, transferring into a flask after 10min, stirring and mixing by using a stirrer for 30min, and fully and uniformly mixing.

Comparative example 1

the auxiliary agent comprises TERSPERSE 2700, alkyl naphthalene sulfonate and white carbon black; the TERSPERSE 2700 is characterized in that: alkyl naphthalene sulfonate: the mass ratio of the white carbon black is 5: 7: 3.

the alkyl naphthalene sulfonate is nekal BX.

The preparation method of the wettable powder has the same specific steps as example 23.

Comparative example No. two

the auxiliary agent comprises a copolymer of methacrylic acid and polyoxyethylene, sodium dodecyl benzene sulfonate and white carbon black; the copolymer of methacrylic acid and polyoxyethylene: sodium dodecylbenzenesulfonate: the mass ratio of the white carbon black is 5: 7: 3.

the copolymer of methacrylic acid and polyoxyethylene is Atlox 4913, british grass.

Comparative example No. three

the auxiliary agent comprises a copolymer of methacrylic acid and polyoxyethylene, alkyl naphthalene sulfonate and white carbon black; the copolymer of methacrylic acid and polyoxyethylene: alkyl naphthalene sulfonate: the mass ratio of the white carbon black is 7: 5: 3.

Performance testing

The suspension percentage and fineness of the wettable powders of example 23 and comparative examples 1 to 3 were measured, and the results are shown in the following table.

Measurement of suspension percentage: measured according to the method of GB/T14825-2006.

Determination of the wetting time: measured according to the method of GB/T5451-2001

Examples	Suspension Rate (%)	Wetting time(s)
			Example 23	80.35	38
Comparative example 1	68.18	51
			Comparative example 2	75.45	67
Comparative example 3	82.65	73

The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in other forms, and any person skilled in the art may modify or change the technical content disclosed above into an equivalent embodiment with equivalent changes, but all those simple modifications, equivalent changes and modifications made on the above embodiment according to the technical spirit of the present invention still belong to the protection scope of the present invention.

Claims

1. The insecticidal composition is characterized by comprising diatomite and alkaloid with specific structures, wherein the mass ratio of the diatomite and the alkaloid with the specific structures is (1:60) - (400:1), the diatomite with the specific structures is diatomite with a saw-tooth honeycomb structure, and the alkaloid is matrine or veratrine.

2. The insecticidal composition according to claim 1, wherein the diatomaceous earth and the alkaloid of the specific structure have a mass ratio of (1:60) - (350:1), (1:60) - (300:1), (1:60) - (250:1), (1:60) - (200:1), (1:60) - (150:1), (1:60) - (100:1), (1:60) - (50:1), (1:60) - (1:1), (1:50) - (400:1), (1:40) - (400:1), (1:30) - (400:1), (1:20) - (400:1), (1:10) - (400:1), or (1:1) - (400: 1).

3. The insecticidal composition of claim 2, wherein the diatomaceous earth and the alkaloid of a particular structure are in a mass ratio of one of 400:1, 350:1, 300:1, 250:1, 200:1, 150:1, 100:1, 50:1, 1:5, 1:10, 1:15, 1:20, 1:25, 1:30, 1:35, 1:40, 1:45, 1:50, and 1: 60.

4. An insecticidal composition according to any one of claims 1 to 3, wherein said composition is formulated as a wettable powder, water dispersible granules, aqueous emulsion, soluble powder, suspension or granules.

5. The insecticidal composition according to claim 4, wherein the insecticidal composition is a wettable powder containing the following components in percentage by mass: 50-90% of diatomite and alkaloid with specific structures and 10-50% of auxiliary agent.

6. An insecticidal composition according to claim 5 wherein said adjuvant comprises a copolymer of methacrylic acid and polyoxyethylene.

7. An insecticidal composition according to claim 6 wherein said methacrylic acid comprises methacrylic acid and/or methyl methacrylate.

8. The insecticidal composition of claim 7 wherein said adjuvant further comprises an alkyl naphthalene sulfonate.

9. A process for the preparation of an insecticidal composition according to any one of claims 5 to 8, comprising the steps of: according to the design formula, the auxiliary agent, the alkaloid and the diatomite with the specific structure are stirred and mixed uniformly to obtain the product.

10. Use of an insecticidal composition according to any one of claims 1 to 8 for the control of citrus red spiders, citrus scale insects, frankliniella occidentalis, whitefly, liriomyza sativae, prodenia litura, tea leafhopper, tea yellow thrips, tea looper, lygus lucorum, grape aphid.