CN111264380B - Breeding method of colored rice two-line hybrid rice - Google Patents

Breeding method of colored rice two-line hybrid rice Download PDF

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CN111264380B
CN111264380B CN202010206105.8A CN202010206105A CN111264380B CN 111264380 B CN111264380 B CN 111264380B CN 202010206105 A CN202010206105 A CN 202010206105A CN 111264380 B CN111264380 B CN 111264380B
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CN111264380A (en
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吴殿星
章思妍
陈霄雨
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Zhejiang University ZJU
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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Abstract

The invention discloses a breeding method of colored rice two-line hybrid rice, which comprises the following steps: colored rice+CAs male parent, and two-line sterile line S as female parent, carrying out hybridizationCrossing and backcrossing with female parent to obtain BCnF2Seed generation; selecting male sterile plants, testing and crossing with two-line restorer line R in pairs, and selecting homozygous sterile line plants with colored seed coats and corresponding hybrid seeds; detecting the heterosis of the seeds, selecting and reserving the hybrid plants and the sterile line plants with the heterosis to obtain the seeds of the sterile line of the colored rice; identifying the fertility, yield, quality and resistance of the sterile line to obtain excellent sterile line plants of the colored rice; breeding seeds to obtain the colored rice two-line hybrid system. The present invention makes the colored rice and two-line sterile line of the examined hybrid rice undergo the processes of hybridization and backcross, and utilizes the restoring line to screen two-line sterile line with colored seed coat and heterosis so as to obtain the invented colored rice hybrid rice and its sterile line not only possess colored seed coat, but also possess the advantages of high yield, high quality and disease resistance.

Description

Breeding method of colored rice two-line hybrid rice
Technical Field
The invention relates to the technical field of rice breeding, in particular to a breeding method of colored rice two-line hybrid rice.
Background
The colored rice refers to brown rice with color and luster, and mainly comprises black, purple, red, yellow, green and the like. The colored rice is brown rice, contains seed coats, rice embryos and aleurone layers, so that the content of protein, fat, amino acid, vitamin and mineral trace elements of the colored rice is obviously higher than that of the white rice (polished rice) which is eaten daily, and the colored rice is rich in natural pigment and various physiological active substances and very accords with the traditional food culture of 'food and medicine' in China. For example: in folk, the black rice is called as the longevity rice, and has the theory of tonifying when meeting black; red rice is commonly used for enriching blood, can nourish yin, tonify kidney, invigorate spleen and stimulate appetite, and is a health food for delaying senility.
Under the background of worsening of the current ecological environment, obviously different life styles and obviously faster rhythm of people, the dietary structure is changed greatly, various chronic diseases such as three highs, atherosclerosis, coronary heart disease, cancer and the like occur frequently year by year, so that the people feel painful to the society and families, and the fashionable health pursuit becomes good, full and healthy. The colored rice is more complete in nutrition and contains physiological active substances capable of preventing various chronic diseases, so that the colored rice is a health choice for a plurality of consumers.
The colored rice in China has rich quality resources, and particularly has more local varieties in Yunnan, Guizhou, Sichuan, Hubei, Guangxi, Jiangsu and other places. However, the colored rice varieties mainly comprise conventional rice varieties, including the germplasm approved by individual varieties, and have the problems of high plant type, poor tillering capacity, low fruiting rate, incomplete seeds, poor resistance and the like. As the hybrid rice becomes a widely applied variety in rice production in China, the yield of the colored rice is obviously relatively low compared with the common conventional rice and particularly the hybrid rice which are produced and popularized.
Therefore, breeding research of hybrid varieties of colored rice is carried out by existing breeding workers, for example, Shibang et al (initial detection of restoration of 23 red rice varieties in different ecological rice growing areas of Guizhou, southwest agriculture, 04 year 2013) are used for clearing the restoration capacity of red rice in different ecological rice growing areas of Guizhou on different cytoplasmic three-line sterile lines, screening red rice resources with restoration genes for heterosis utilization, and carrying out hybridization testing and matching research on 23 red rice serving as materials and 3 different cytoplasmic three-line sterile lines so as to screen red rice restoring lines. The research and utilization of the breeding of the black hybrid japonica rice, Jiangsu agricultural science, 2009, and the like, determine the recovery and preservation relationship of partial colored rice resources with slightly good properties through backcrossing, hybridization and the like, and simultaneously transform the resources, so that the breeding of the new variety of the sterile line of the colored rice is performed, and tests show that the black rice three-line hybrid rice prepared by the black rice A has good plant type, good resistance and high yield, and can improve the relevant poor properties of the black rice. Zjuncai and Xiaoyeqing (black rice three-line matched breeding first report, Jiangxi agriculture report, 2000 year 01) adopt an artificial method for making and restoring to breed 2 male sterile lines (big black A and blood black A) and 3 restoring lines (black gray No. 1, black restoring No. 2 and black restoring No. 3) of black rice, and carry out matching tests. Huangping and the like (round-grained purple rice nuclear-cytoplasmic interaction sterile line brocade 315A, seeds, the 1 st stage in 2017) further hybridize with the self-bred new line (72B-2/purple rice// XR 11) by utilizing the self-bred sterile line (Chuanxiang 29A/round-grained soft// IRRI 2B/koji 3) and polymerize different excellent genes to enrich the genetic background, thus obtaining the round-grained purple rice nuclear-cytoplasmic interaction new sterile line 315A. At present, only a few colored rice hybrid rice passes provincial approval or test reports, red rice mainly comprises Yunnan hybrid 701 (sterile line D5A, red rice restorer line Yunnan Kunxiang No. 4), Jinnong No. 3 (sterile line Jinnong 3A, red rice restorer line Jinhui No. 3), Jinyouhong (sterile line 23A, cold-resistant red rice restorer line Red zero-4), Gaoyouhong 88 (sterile line Gaofeng A, red rice restorer line R hong 88), Yuyou hong 9 (sterile line Yuqing 802A, red rice restorer line Yuqing 9341), II you 132 (sterile line II-32A as female parent, red rice restorer line), Taiyou hong 1256 (sterile line Longtepu A, red rice restorer 1256), black you No. 1 (sterile line Zhenshan 97A, black rice restorer line 3350) and black rice 906 (sterile line 4D983, sterile line 906 and black rice restorer line 703D 3) are combined with the reported test reports, black rice restorer line 4D 703). The above mentioned varieties, no matter red rice or black rice hybrid varieties, the sterile line is common non-colored rice, but only the restorer line is red rice or black rice, limited by the hereditary property, the seed coat of these varieties is not obvious enough, for example, the brown rice of red rice hybrid rice is reddish brown, atypical red; the brown rice of the black rice hybrid rice is black or black brown, although the yield, the resistance and the like are improved compared with the conventional variety of the colored rice, the functional nutrition is reduced, and compared with the super variety of the hybrid rice, the yield and the quality are not superior, so that the production and the application of consumers are restricted.
Therefore, the invention provides a breeding method of colored rice two-line hybrid rice, and aims to provide the colored rice hybrid rice with higher yield, better quality and dual purposes.
Disclosure of Invention
The invention provides a breeding method of colored rice two-line hybrid rice, and the colored rice hybrid rice obtained by the breeding method not only has colored seed coats, but also has the advantages of high yield, high quality and disease resistance.
The specific technical scheme is as follows:
a breeding method of colored rice two-line hybrid rice comprises the following steps:
(1) hybridizing the colored rice C as male parent and the two-line sterile line S as female parent, and backcrossing the obtained filial generation and female parent for n times to obtain BCnF1Generation, BCnF1After generation selfing, BC is harvestednF2Seed generation;
(2) observation of BCnF2Selecting homozygous seed with colored seed coat, planting according to individual plant, selecting male sterile plant during flowering to obtain male sterile plant S'1(ii) a Preparing male sterile plant S'1Testing and crossing with two-line restoring line R in pairs to obtain hybrid F1Generation;
then male sterile plant S'1Performing cutting regeneration, and selecting sterile line plant S 'with fertility converted into fertility'2Observing sterile line plant S'2Selecting seed coat color of seed to obtain homozygous sterile line plant S 'with colored seed coat'3And from said hybrid F1Selecting sterile line plant S 'from the generation'3Hybrid F obtained after paired test crossing with restorer R1a；
(3) Mixing the hybrid F obtained in the step (2)1aAnd sterile line plant S'3The seeds of (1) are planted in pairs and observed for hybrid F1aPlant and sterile line plant S'3Selecting hybrid plants with hybrid vigor and corresponding sterile plants to obtain hybrid F1bPlant and sterile line plant S'4(ii) a Then observing sterile line plant S'4The fertility of (2) is selected and reserved to obtain a sterile line plant S 'with complete sterility and stable homozygous'5；
Then, the sterile line plant S'5Retesting with restorer R to obtain hybrid F when plant is mature1cObserve hybrid F1cThe seed coat color, the quality of the seed grains are detected, and homozygous high-quality hybrid F with colored seed coats is obtained by selecting and reserving the seed grains1dAnd selecting hybrid F1dObtaining sterile line plants S 'from the corresponding sterile line plants'6Preparing sterile plant S'6Performing pile cutting regeneration, and selecting and reserving sterile line plant S 'with fertility converted into fertility'7Harvesting sterile line plant S'7The seed of (1);
(4) planting sterile line plant S'7The seeds of (1) are subjected to indoor and outdoor fertility identification, and sterile line plants with sterility conforming to the characteristics of the sterile line, homozygous seeds, high quality and colored seed coats are selected to obtain sterile line plants S'8(ii) a Detecting sterile line plant S'8The seed coat color, yield, rice quality and resistance of the hybrid seeds obtained after the re-testing with the restorer line R are selected to obtain excellent colored rice two-line sterile line plants with colored seed coats, high yield, good rice quality and strong resistanceS'9；
(5) Utilizing colored rice two-line sterile line plant S'9Breeding the colored rice two-line sterile line with the restorer line R to obtain the colored rice two-line sterile line S+C(ii) a Establishment of two-line sterile line S of colored rice+CRestorer line R and sterile line S+CHybrid rice variety S hybridized with restorer line R+CX R to form a colored rice two-line hybrid system.
S 'related in the breeding method'1～S'9"sterile line plant population is obtained in the breeding process, the number of the plants is more than 1; and "S+C"is 1 sterile line plant obtained after the completion of the breeding.
Further, the colored rice C is any one of black rice, red rice and purple rice.
Further, the colored rice C is Zhongze black treasure.
The Zhongze Heizhen is a giant embryo japonica rice variety of black rice cultivated by Zhejiang university and Zhongze biotechnology limited company; the variety has already applied for accepting new variety protection to new variety protection offices of plants in Ministry of agriculture, and the application number is as follows: 20150962.5, available from Zhongze Biotechnology, Inc.
Further, the two-line sterile line S and the restorer line R are conventional high-quality and high-yield two-line hybrid rice combinations.
Further, the two-line sterile line S is Guangzhan 63-4S; and the restorer line R is Yang rice No. 6.
The rice can be widely used for 63-4S, is a northern hybrid japonica rice research center and is cooperated with Hefeifeng-Fengle breeding for breeding, can be given for obtaining or purchasing, and can also be claimed from a national germplasm resource bank.
The restorer line R Yang rice No. 6 is a public material, is bred by Riseudo and Riseudo Seisakusho agricultural academy, can be obtained or purchased in a way of benefiting gifts, and can also ask for the key of a national germplasm resource bank.
The invention breeds the colored rice two-line sterile line S according to the rice breeding technology and flow and the overall evaluation of the sterile property, the heterosis, the quality, the resistance and other properties+CRestorer R and new hybrid rice variety S+CX R, specifically including：l)S+CThe sterile characteristics of the line are similar to those of S, the line has high outcrossing seed setting rate and high seed reproduction yield, and meets the identification standard of two-line sterile lines; 2) s+CThe yield, quality, resistance and other levels of the multiplied by R are similar to the hybrid rice varieties of the sterile line S and the restorer line R.
Further, in the step (1), n is 3-5 times.
Further, in step (3), the evaluation index with heterosis includes: yield, rice quality and pest resistance.
Furthermore, the yield is more than or equal to 500 kg/mu, the rice quality reaches the national third-level standard and above, and the bacterial leaf blight resistance is taken as the judgment standard for heterosis.
Further, in the step (4), the sterile line is characterized in that the outcrossing seed setting rate is more than or equal to 40%; the seed reproduction yield is more than or equal to 200 kg.
Compared with the prior art, the invention has the following beneficial effects:
(1) the present invention makes the colored rice and two-line sterile line of the examined hybrid rice undergo the processes of hybridization and backcross, and utilizes the restoring line to screen two-line sterile line with colored seed coat and heterosis so as to obtain the invented colored rice hybrid rice and its sterile line not only possess colored seed coat, but also possess the advantages of high yield, high quality and disease resistance.
(2) Compared with the colored rice hybrid varieties prepared by the conventional breeding approved colored rice restorer line, the colored rice hybrid varieties prepared by the two-line sterile line of the colored rice obtained by the invention have more beautiful and more obvious seed coat color, are not reddish brown like brown rice of red rice hybrid rice, and are atypical red; unlike brown rice of black rice hybrid rice, which is black or blackish brown, functional nutrition is rather reduced although yield, resistance, and the like are improved.
(3) The invention selects a new combination of high-yield, high-quality and disease-resistant target two-line hybrid rice (hybrid rice is Yangliangyou No. 6, sterile line is 63-4S, and restoring line is Yangliangyou No. 6), breeds the colored rice hybrid rice with similar yield, quality and resistance with the target variety Yangliangyou No. 6, the quality of the colored rice hybrid rice is high, and the colored rice hybrid rice has dual purposes, and obviously reduces the production and popularization risks.
(4) The method adopts a colored rice breeding mode with a two-line sterile line as a core, and needs to select a maintainer line first and then transfer the sterile line, thereby obviously shortening the breeding time and shortening the transfer time of continuous 7-8 generations of the three-line sterile line compared with the breeding of the three-line sterile line.
Detailed Description
The present invention will be further described with reference to the following specific examples, which are only illustrative of the present invention, but the scope of the present invention is not limited thereto.
The breeding method provided in the following examples is a breeding method of colored rice hybrid rice based on high-yield, high-quality and disease-resistant two-line hybrid rice Yangliangyou No. 6 (parent of Yangliangyou No. 6 is: female parent Guangzhan 63-4S, male parent Yangliang No. 6).
Wherein Yangliangyou No. 6 is an excellent two-line hybrid rice bred by agricultural scientific research institute in Rixia of Jiangsu. The crops varieties approval committees of Jiangsu province and Guizhou province in 2003 are approved, the crops varieties approval committees of Henan province in 2004 are approved, the crops varieties approval committees of Hubei province in 2005 are approved by the national varieties approval committees, and the approval number is national approval rice 2005024. The rice quality is measured by food quality supervision inspection test center of Ministry of agriculture, and the rice quality has the roughness of 80.4%, the whole rice rate of 58.8%, the chalkiness grain rate of 14%, the chalkiness degree of 2.8%, the amylose content of 15.4%, the glue thickness of 83 mm, the length-width ratio of 3.1, and reaches the national standard of three-grade high-quality rice quality. The average yield per mu of the two-year regional test is 555.80 kilograms, and the yield is increased by 4.87 percent compared with the II best 725. Resisting bacterial leaf blight. Available from Beijing gold agriculture and China, Inc.
The rice can be widely used for 63-4S, is a northern hybrid japonica rice research center and is cooperated with Hefeifeng-Fengle breeding for breeding, can be given for obtaining or purchasing, and can also be claimed from a national germplasm resource bank.
The restorer line R Yang rice No. 6 is a public material, is bred by Riseudo and Riseudo Seisakusho agricultural academy, can be obtained or purchased in a way of benefiting gifts, and can also ask for the key of a national germplasm resource bank.
The Zhongze Heizhen is a giant embryo japonica rice variety of black rice cultivated by Zhejiang university and Zhongze biotechnology limited company; the variety has already applied for accepting new variety protection to new variety protection offices of plants in Ministry of agriculture, and the application number is as follows: 20150962.5, available from Zhongze Biotechnology, Inc.
Example 1
A breeding method of colored rice two-line hybrid rice comprises the following specific steps:
(1) group construction: uses the rice black rice line Zhongze black rice (i.e. coloured rice)+C) As a male parent, it accounts for 63-4S (i.e.: two-line sterile line S) as female parent, hybridizing to obtain filial generation, backcrossing with Guangzhan 63-4S for 4 times to obtain BC4F1Generation plant 2, BC4F1Selfing for 1 time, and harvesting BC4F2Seed generation;
(2) selecting test cross: observation of BC4F21202 seeds are substituted for the seeds, homozygous seeds with colored seed coats are selected and reserved, and the seeds are planted according to a single plant, and 322 plants are planted in total; at the time of planting to flowering, 53 plants of male sterile among 322 plants were selected to obtain male sterile plants S'1(ii) a Preparing male sterile plant S'1Test cross with Yangdao No. 6 (restoring line R) to obtain hybrid F1Generation;
then 53 male sterile plants S'1Performing pile cutting regeneration, and selecting sterile line individual plant S 'with fertility converted into fertility'2Observing individual plant S 'of sterile line'2Seed coat color of the seeds, 26 sterile line single plants S 'which are homozygous and have colored seed coats are selected and remained'3And from hybrid F1Selecting individual plant S 'of the 26 sterile lines in the generations'3Hybrid F obtained after paired test crossing with Yangdao No. 61a；
(3) And (3) detection and verification: mixing the hybrid F obtained in the step (2)1aAnd 26 sterile line individual plants S'3The seeds of (1) are planted in pairs and observed for hybrid F1aPlants and sterile plants' S3The hybrid vigor of the rice is selected from 16 plants which have the hybrid vigor similar to that of No. 6 Yangliaoyou (the yield is more than or equal to 500 kg/mu, the rice quality reaches the national third-level standard and above, and the bacterial blight resistance is taken as the judgment standard for the hybrid vigor)Hybrid plants and corresponding sterile plants to obtain hybrid F1bPlant and sterile line plant S'4(ii) a Then observing sterile line plant S'4The fertility of (2) is selected to leave a sterile line plant S 'with complete sterility and stable homozygous'513 strains;
then 13 sterile line plants S'5Retesting with restorer line Yanggao No. 6, obtaining hybrid F when plant is mature1cObserve hybrid F1cThe seed coat color, the quality of the seed grains is detected, and 6 homozygous and high-quality hybrids F with colored seed coats are selected and remained1dAnd selecting hybrid F1dObtaining sterile line plants S 'from the corresponding sterile line plants'6Preparing sterile plant S'6Performing pile cutting regeneration, and selecting and reserving sterile line plant S with fertility converted into fertility7Harvesting sterile line plant S'76 seeds of (2), namely 6 seeds of the sterile line of the colored rice;
(4) and (3) identifying fertility, yield, quality and resistance: 6 sterile line plants S 'are planted'7The seeds are subjected to indoor and outdoor fertility identification, sterile plants which have sterility conforming to the characteristics of the sterile line (the outcrossing seed setting rate is more than or equal to 40 percent and the seed reproduction yield is more than or equal to 200kg) and are homozygous, high-quality and colored in seed coats are selected and obtained to obtain 4 sterile plants S'8(ii) a Detecting sterile line plant S'8The seed coat color, yield, rice quality and resistance of the hybrid seeds obtained after the re-testing with the restorer line R are selected to obtain 3 excellent colored rice two-line sterile line plants S 'with colored seed coats, high yield, good rice quality and strong resistance'9；
(5) Breeding and seed production: for 3 colored rice two-line sterile line plants S'9Breeding seeds, and breeding seeds with the restorer line Yanggao No. 6, and finally selecting the colored rice two-line sterile line S with high breeding seed yield and low cost+C1 (ZD-br-1S).
(6) Two-line hybrid system of colored rice two-line hybrid rice: establishing a colored rice two-line hybrid system consisting of a colored rice two-line sterile line ZD-br-1S, a restorer Yanggao No. 6 and a new hybrid rice variety ZD-br-1S multiplied by Yanggao No. 6 for the hybrid of the sterile line ZD-br-1S and the restorer Yanggao No. 6.
The joint product ratio test shows that the bred black rice hybrid rice variety accounts for 63-4S+CYanggao No. 6 has the same group yield, edible quality and disease resistance level as Yangliang No. 6, but has the black degree as high as 93 percent, the black rice pigment E content of 2.62 and obviously better nutritional quality, and can be used as special rice and high-quality and high-yield rice in the final application, and the characteristic of taking multiple utilization into consideration is very favorable for large-area popularization, increasing the benefit and obviously avoiding potential risks; compared with the Zhongze black pearl, the yield is obviously higher, the resistance is obviously better, and the nutrition and the edible quality are similar; compared with hybrid varieties, the hybrid varieties account for 63-4S/Zhongze Heizheng, and the quality, the resistance and the yield are all remarkably superior, which shows that the effect of the method is obvious.
Compared with the original parent and the prepared hybrid rice variety, the hybrid rice variety of the black rice accounts for 63-4S+CThe cadmium content of Yangdao No. 6 is higher than that of parent and combination, i.e. the cadmium content is low. Cadmium is the most typical harmful heavy metal and is currently the most feared nutrient for the public. The cadmium content is low, which shows that the bred variety has higher edible safety.
Specific comparisons of seed coat color, black rice pigment, yield, quality and disease resistance of the individual varieties are shown in Table 1.
TABLE 1 comparison of index traits of Yangliangyou No. 6 improved black hybrid rice varieties
Figure BDA0002421191630000061
Figure BDA0002421191630000071
。

Claims (5)

1. A breeding method of colored rice two-line hybrid rice is characterized by comprising the following steps:
(1) hybridizing the colored rice C as male parent and the two-line sterile line S as female parent, and backcrossing the obtained filial generation and female parent for n times to obtain BCnF1Generation, BCnF1After generation selfing, BC is harvestednF2Seed generation;
(2) observation of BCnF2Selecting homozygous seed with colored seed coat, planting according to individual plant, selecting male sterile plant during flowering to obtain male sterile plant S'1(ii) a Preparing male sterile plant S'1Testing and crossing with two-line restoring line R in pairs to obtain hybrid F1Generation;
then male sterile plant S'1Performing cutting regeneration, and selecting sterile line plant S 'with fertility converted into fertility'2Observing sterile line plant S'2Selecting seed coat color of seed to obtain homozygous sterile line plant S 'with colored seed coat'3And from said hybrid F1Selecting sterile line plant S 'from the generation'3Hybrid F obtained after paired test crossing with restorer R1a；
(3) Mixing the hybrid F obtained in the step (2)1aAnd sterile line plant S'3The seeds of (1) are planted in pairs and observed for hybrid F1aPlant and sterile line plant S'3Selecting hybrid plants with hybrid vigor and corresponding sterile plants to obtain hybrid F1bPlant and sterile line plant S'4(ii) a Then observing sterile line plant S'4The fertility of (2) is selected and reserved to obtain a sterile line plant S 'with complete sterility and stable homozygous'5；
Then, the sterile line plant S'5Retesting with restorer R to obtain hybrid F when plant is mature1cObserve hybrid F1cThe seed coat color, the quality of the seed grains are detected, and homozygous high-quality hybrid F with colored seed coats is obtained by selecting and reserving the seed grains1dAnd selecting hybrid F1dObtaining sterile line plants S 'from the corresponding sterile line plants'6Preparing sterile plant S'6Performing pile cutting regeneration, and selecting and reserving sterile line plant S 'with fertility converted into fertility'7Harvesting sterile line plant S'7The seed of (1);
(4) planting sterile line plant S'7The seeds of (1) are subjected to indoor and outdoor fertility identification, and the sterility is selected and kept to accord with the sterilityObtaining sterile line plants S 'from sterile line plants with line characteristics, homozygous seeds, high quality and colored seed coats'8(ii) a Detecting sterile line plant S'8The seed coat color, the yield, the rice quality and the resistance of the hybrid seeds obtained after the re-testing with the restorer line R are selected to obtain excellent colored rice two-line sterile line plants S 'with colored seed coats, high yield, good rice quality and strong resistance'9；
(5) Utilizing colored rice two-line sterile line plant S'9Breeding the colored rice two-line sterile line with the restorer line R to obtain the colored rice two-line sterile line S+C(ii) a Establishment of two-line sterile line S of colored rice+CRestorer line R and sterile line S+CHybrid rice variety S hybridized with restorer line R+CA colored rice two-line hybrid system consisting of xR;
the colored rice C is Zhongze black pearl; the two-line sterile line S is 63-4S; and the restorer line R is Yang rice No. 6.
2. The method for breeding two-line hybrid rice of colored rice as claimed in claim 1, wherein in step (1), n is 3 to 5 times.
3. The method for breeding two-line hybrid rice of colored rice as claimed in claim 1, wherein the index for judging heterosis in step (3) comprises: yield, rice quality and disease resistance.
4. The method for breeding two-line hybrid rice of colored rice as claimed in claim 3, wherein the rice quality is up to the national third and above standard with the yield of 500 kg/mu or more and the bacterial leaf blight resistance as the criterion for the heterosis.
5. The method for breeding two-line hybrid rice of colored rice as claimed in claim 1, wherein in step (4), said sterile line is characterized by having a cross-maturing rate of not less than 40%; the seed reproduction yield is more than or equal to 200 kg.
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CN114402993B (en) * 2021-12-27 2023-04-07 浙江大学 Method for breeding high-quality color hybrid rice
CN114451291B (en) * 2021-12-29 2023-09-08 海南浙江大学研究院 Breeding method of multi-nutrition polymerized rice
CN114424745B (en) * 2021-12-29 2023-04-07 浙江大学 Breeding method of micro-nutrition reinforced rice
CN114431134B (en) * 2022-01-17 2023-06-23 浙江大学 Breeding method of hybrid color rice
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