CN111249323A

CN111249323A - Folium Artemisiae Argyi extract and its application in skin external preparation

Info

Publication number: CN111249323A
Application number: CN202010197032.0A
Authority: CN
Inventors: 陈默; 祝乐; 赵亚; 孙懿; 李玲玉; 陈媛祺
Original assignee: Shanghai Jahwa United Co Ltd
Current assignee: Shanghai Jahwa United Co Ltd
Priority date: 2020-03-19
Filing date: 2020-03-19
Publication date: 2020-06-09

Abstract

The invention provides an application of folium artemisiae argyi extract in skin moisturizing and/or skin barrier, wherein the extraction process of the folium artemisiae argyi extract comprises the following steps: decocting, extracting, or a combination thereof. The moisturizing and/or barrier effect of the folium artemisiae argyi extract is realized by improving the expression of genes related to moisturizing and/or barrier efficacy. The invention also discloses application of the skin external preparation in preparing the skin external preparation with the effects of moisturizing the skin and/or shielding the skin.

Description

Folium Artemisiae Argyi extract and its application in skin external preparation

Technical Field

The invention belongs to the field of phytochemistry, and particularly relates to a folium artemisiae argyi extract and application thereof in a skin external preparation or a cosmetic.

Background

Skin refers to the tissue of the body surface outside the muscles, is the largest organ of the human body, and mainly plays a role in protecting the body, removing sweat, feeling cold and heat, pressure and the like. The skin covers the whole body, protects tissues and organs in the human body from being invaded by external substances such as pathogenic microorganisms and the like and mechanical injuries such as physics and the like, and effectively resists external stimulation; the skin also keeps the loss of water, electrolytes, nutrient substances and the like of the human body, effectively maintains the stability of the internal environment of the human body and is a natural barrier for protecting the human body.

Healthy skin is soft, smooth, and elastic, largely due to the abundant moisture in the skin, which is high and accounts for 18-20% of the total body mass. When the moisture content of the skin is lower than 10%, the skin becomes dry, rough and even chapped, and the skin loses water due to dry environment, unbalanced water and oil of the skin, excessive cleaning and the like. When the skin loses water excessively, the skin barrier is broken, so that the skin barrier cannot play a role in sufficiently retaining water. The skin does not have sufficient moisture for a long time, so that the skin is dry, the skin aging is accelerated, and various skin problems are caused; in addition, the sufficient water content of the skin is a prerequisite for improving the physiological environment of the skin and promoting the metabolism of the skin. Therefore, maintaining skin moisturization is particularly important in daily skin care.

Moisture retention is a basic appeal of skin care, and the moisture retention efficacy is the most basic function of cosmetics. The skin barrier and the skin moisturizing function are closely related. Barrier function generally refers to the physical barrier structure of the epidermis, especially the stratum corneum, and is of great physiological significance in maintaining the stability of the internal environment of the body and resisting the harmful invasion of the external environment. The daily functions of isolating and protecting, regulating and controlling the absorption of foreign substances through skin, preventing the evaporation of skin moisture and the like are achieved, and further the functions of moisturizing, regulating and resisting inflammation are achieved. Therefore, if the skin is frequently exposed to various exogenous stimuli, such as allergens, irritant solvents, ultraviolet rays, microorganisms, particles and a series of stimuli, the integrity of the stratum corneum is damaged by the low resistance, and finally the barrier function of the skin is damaged, so that skin diseases such as dry skin, aging, pigmentation atopic dermatitis, eczema, psoriasis, ichthyosis, solar dermatitis and the like, skin sensitivity, irritant dermatitis, hormone-dependent dermatitis and the like are caused.

Studies have shown that there are genes whose expression in the epidermis is closely related to moisturization and barrier. For example, aquaporin (AQP-3) is a protein located on the cell membrane and can significantly increase the permeability of cell membrane water. Aquaporins between epidermal cells constitute the major pathway for intercellular water transport. The filaggrin FLG is an important factor involved in the skin barrier, and has the main functions of participating in the differentiation of epidermal cells and the formation of the skin barrier. At the same time, FLG is able to break down to produce large amounts of natural moisturizing factors, which are also critical for maintaining skin hydration and skin barrier. The lorin gene, LOR, is a major component of the keratin membrane envelope (i.e., the "brick" in the brick wall structure) and plays an important role in the barrier function of the epidermis.

With the growing concern of consumers on their own health and safety in recent years, plant-based moisturizers have become hot to the hands against many biochemically synthesized cosmetic efficacy raw materials. Chinese plants are abundant in resources, and particularly represent Chinese herbal medicines. In ancient China, people used Chinese herbal medicines to beautify and care skin.

Folium Artemisiae Argyi is derived from dried leaf of Artemisia argyi Levl. et Vant of Compositae, and has effects of warming channels, stopping bleeding, dispelling cold, relieving pain, and treating skin pruritus. The folium artemisiae argyi is a traditional Chinese medicinal material and is a plant resource used as both medicine and food. Folium Artemisiae Argyi contains volatile oil, triterpenes, and other chemical components, and also contains abundant flavonoids. Modern pharmacological research shows that the folium artemisiae argyi flavonoid compound has various pharmacological activities of oxidation resistance, tumor resistance, inflammation resistance, bacteriostasis and the like. In the past, researches usually focus on the antibacterial, antiviral and anti-inflammatory effects of folium artemisiae argyi and folium artemisiae argyi flavone, and the antibacterial effect is mainly used in cosmetics.

CN105535507A discloses a skin-care traditional Chinese medicine composition containing folium artemisiae argyi extract and a skin-care product containing the traditional Chinese medicine composition, wherein the skin-care product has the effects of repairing regeneration, whitening, moisturizing and delaying aging.

CN110051592A discloses an essence moisturizing spray containing artemisia argyi extract, which has antioxidant and bactericidal effects.

CN109125191A discloses a cosmetic additive of folium artemisiae argyi with moisturizing effect; the folium artemisiae argyi-containing additive can improve the skin barrier function, and does not represent that the folium artemisiae argyi has the function of improving the skin barrier.

CN109953909A discloses an antibacterial moisturizing hand sanitizer containing folium artemisiae argyi extract.

CN109425191A discloses a cosmetic additive with good moisturizing effect.

The document 'development of novel folium artemisiae argyi bacteriostatic hand sanitizer' discloses that folium artemisiae argyi extract has certain bacteriostatic effect and has bactericidal performance on escherichia coli, staphylococcus aureus and the like.

In all of these patent applications and documents, although it is mentioned that the folium artemisiae argyi is added to prepare a hand sanitizer or a spray, and the product has the functions of moisturizing, improving the skin barrier and bacteriostasis, the folium artemisiae argyi is not only added as a raw material, but is added in a compound way, and other components are humectants such as shea butter, xylitol glucoside, betaine, aloe and the like, and the moisturizing and skin barrier improving effects of the folium artemisiae argyi cannot be inferred.

Therefore, no research on moisturizing and/or barrier aspects of folium artemisiae argyi is currently available, and no report on the influence of expression of related genes such as aquaporin 3(AQP3), Loricrin (LOR) and Filaggrin (FLG) is available.

The application takes the folium artemisiae argyi as a research object for the first time, and the effect of the folium artemisiae argyi extract on the aspects of skin moisturizing and barrier is investigated.

Disclosure of Invention

The invention discovers for the first time that the folium artemisiae argyi extract has excellent moisturizing and barrier effects and is suitable to be added into a skin external preparation as an effect additive to achieve the moisturizing and barrier maintenance effects.

In one aspect, the present invention provides a use of an artemisia argyi extract in skin moisturizing and/or skin barrier, wherein the extraction process of the artemisia argyi extract comprises: decocting, extracting, or a combination thereof.

In a preferred embodiment, the extraction solvent is selected from: water, ethanol, and combinations thereof.

In a preferred embodiment, the extraction process of the artemisia argyi extract further comprises alcohol precipitation, membrane filtration or a combination thereof.

In a preferred embodiment, the weight ratio between the mugwort leaves and the extraction solvent in the extraction process is equal to or greater than 1: 10.

In a preferred embodiment, the skin moisturization and/or skin barrier effect is achieved by improving the expression of a moisturization and/or barrier efficacy-related gene. In a more preferred embodiment, the moisturizing and/or barrier efficacy-related genes include aquaporin 3, loricrin, filaggrin, and combinations thereof.

In another aspect, the present invention provides a use of an artemisia argyi extract for preparing a skin external preparation having skin moisturizing and/or skin barrier effects, wherein the extraction process of the artemisia argyi extract comprises: decocting, extracting, or a combination thereof.

In a preferred embodiment, the external preparation for skin is selected from: face cleaning lotion, cosmetic water, lotion, cream and facial mask.

In a preferred embodiment, the external preparation for skin comprises 0.001 to 20% by weight of the mugwort leaf extract.

Drawings

FIG. 1 shows the effect of the Artemisia princeps Pampanini extract of example 2 on the promotion of aquaporin AQP 3. In the figure, "extract of artemisia argyi of example 2" is the extract of artemisia argyi of example 2, and "BC" represents the blank control.

FIG. 2 shows the promoting effects of the mugwort extract of example 2 on loricrin LOR and filaggrin FLG. In the figure, "extract of artemisia argyi of example 2" is the extract of artemisia argyi of example 2, and "BC" represents the blank control.

Detailed Description

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are described herein. For the purposes of the present invention, the following terms are defined below.

The term "about" as used herein refers to an amount, level, value, dimension, size, or amount that differs by up to 30%, 20%, or 10% as compared to the amount, level, value, dimension, size, or amount of a reference. The percentages used herein are by weight unless otherwise indicated.

Throughout the specification and claims, unless the context requires otherwise, the word "comprise", and variations such as "comprises" and "comprising", will be understood to imply the inclusion of a stated integer or step or group of integers or steps but not the exclusion of any other integer or step or group of integers or steps.

The invention discovers the effects of the folium artemisiae argyi extract on the aspects of moisture preservation and barrier maintenance for the first time, and can promote the expression of moisture preservation and barrier related genes such as aquaporin 3(AQP3), Loricrin (LOR) and Filaggrin (FLG). Therefore, the folium artemisiae argyi extract can be used as an effect additive to be added into a skin care product for skin care, and is helpful for moisturizing the skin and keeping the skin bottom tough, caring and repairing the skin barrier, and improving the problems of dry skin, water shortage, skin barrier damage and the like.

Therefore, the present invention aims to study the application of the artemisia leaf extract in the aspects of skin moisturizing and barrier maintenance. In the research, the folium artemisiae argyi extract is found to have the effect of promoting the expression of moisturizing and barrier related genes aquaporin 3(AQP3), Loricrin (LOR) and Filaggrin (FLG). Therefore, the folium artemisiae argyi extract can be used as an effect additive to be added into a skin external preparation, so that the effects of moisturizing the skin and keeping the skin bottom tenaciously, caring and repairing the skin barrier are achieved, and the problems of dry skin, water shortage, skin barrier damage and the like are solved.

Preparation method of folium Artemisiae Argyi extract

In a specific embodiment, the mugwort extract is prepared by solvent extraction. In a preferred embodiment, the solvent used in the extraction process is water, such as deionized water.

In a specific embodiment, the mugwort extract is prepared by a solvent decoction method. In a preferred embodiment, the solvent used in the extraction process is water, such as deionized water.

In a specific embodiment, the extraction process further comprises an alcohol precipitation step with ethanol.

In a particular embodiment, the extraction process further comprises a membrane filtration step. In a preferred embodiment, the membrane filtration step employs a ceramic membrane having a pore size of 50 nm.

In a particular embodiment, the weight ratio between the mugwort leaves and the extraction solvent during the extraction process is equal to or greater than 1: 10.

Genes associated with moisturizing and/or barrier efficacy

Studies have shown that there are genes whose expression in the epidermis is closely related to moisturization and barrier. For example, aquaporin (AQP-3) is a protein located on the cell membrane that significantly increases the permeability of cell membrane water. Aquaporins between epidermal cells constitute the major pathway for intercellular water transport. For another example, filaggrin FLG is an important factor involved in the skin barrier, and its main functions are involved in the differentiation of epidermal cells and the formation of the skin barrier. At the same time, FLG is able to break down to produce large amounts of natural moisturizing factors, which are also critical for maintaining skin hydration and skin barrier. Loricrin LOR is the main constituent of the keratin membrane envelope (i.e., "brick" in brick wall construction) and plays an important role in the barrier function of the epidermis. In a specific embodiment, the moisturizing effect of the artemisia leaf extract is achieved by improving the expression of a moisturizing efficacy-related gene.

Aquaporins

Aquaporins (Aquaporin, AQP3), also known as aquaporins, are proteins located on cell membranes (integral membrane proteins) that form "tunnels" in the cell membrane that control the passage of water into and out of the cell. The aquaporin as a protein located on a cell membrane can obviously increase the permeability of cell membrane water, and the aquaporin between epidermal cells forms a main pathway for intercellular water transfer.

Loricrine protein

Loricrin (LOR) is a major component of the cornified envelope and plays an important role in the barrier function of the epidermis. The expression regulation of the gene is co-regulated by complex interactions between multiple transcription factors. Mutations in the loricrin gene can lead to loricrinosis, and nuclear translocation of the mutant loricrin interferes with terminal differentiation of keratinocytes, thereby causing the corresponding clinical phenotype. Reduced expression of loricrin also occurs in atopic dermatitis and psoriatic lesions, and its abnormal expression is influenced by associated cytokines.

Silk fibroin

Filaggrin (FLG) is an important component of the stratum corneum, is synthesized by keratinocytes, is distributed in different parts of the stratum corneum, is gradually degraded by enzymes along with the migration process of the keratinocytes, is degraded into small molecular substances required by the stratum corneum of natural moisturizing factors, and plays an important role in the aspects of moisturizing and barrier integrity.

External preparation for skin

The folium artemisiae argyi extract can be used as an additive with moisturizing and/or barrier effects to be applied to a skin external agent. In a specific embodiment, the artemisia argyi extract of the present invention can be applied to cosmetics as an additive having moisturizing efficacy. In a particular embodiment, the cosmetic is selected from: face cleaning lotion, cosmetic water, lotion, cream, jelly and facial mask. Different amounts are added according to different types of preparations.

In some preferred embodiments, the amount of the mugwort extract in the skin external preparation may be 0.001% to 20% (w/w). Preferably 0.01-20% (w/w). More preferably 0.01% to 10% (w/w). More preferably 0.1% to 5% (w/w). In some preferred embodiments of the present invention, the amount of the mugwort leaf extract in the skin external preparation may be 0.001% -10% (w/w), 0.002% -10% (w/w), 0.003% -10% (w/w), 0.01% -10% (w/w), 0.02% -10% (w/w), 0.03% -10% (w/w), 0.1% -10% (w/w), 0.2% -10% (w/w), 0.3% -10% (w/w). In some preferred embodiments of the present invention, the amount of the mugwort leaf extract in the skin external preparation may be 0.001% to 1% (w/w), for example, 0.002% to 1% (w/w), 0.003% to 1% (w/w), 0.01% to 1% (w/w), 0.02% to 1% (w/w), 0.03% to 1% (w/w), 0.1% to 1% (w/w), 0.2% to 1% (w/w), 0.3% to 1% (w/w).

In another aspect of the present invention, there is provided a skin external preparation having moisturizing effect, comprising the moisturizing composition of the present invention and a cosmetically acceptable excipient.

The external preparation for skin is a general concept of all ingredients generally used for the external part of skin, and may be, for example, a cosmetic composition or a pharmaceutical composition. The cosmetic composition can be basic cosmetics, face makeup cosmetics, body makeup cosmetics, hair care cosmetics and the like, and the dosage form of the cosmetic composition is not particularly limited and can be reasonably selected according to different purposes.

The cosmetic composition also contains different cosmetically acceptable media or matrix excipients according to different dosage forms and purposes.

The cosmetically, dermatologically or pharmaceutically acceptable vehicle that can be used in the composition for external application to skin of the present invention is in the form of a water phase, an oil phase, a gel, a wax-in-water emulsion, an oil-in-water emulsion or a water-in-oil emulsion. The aqueous phase is a mixture of one or more water-soluble or dispersible components, which may be liquid, semi-solid, or solid at room temperature (25 ℃). The vehicle includes or may be in the form of a suspension, dispersion or solution in an aqueous or hydro-alcoholic vehicle, which may contain a thickening or gelling agent. The person skilled in the art can select suitable product forms, the components contained therein, based on the knowledge of the person skilled in the art.

The composition may comprise an aqueous phase which may contain water or a mixture of water and at least one hydrophilic organic solvent such as an alcohol, in particular a linear or branched lower monohydric alcohol containing from 2 to 5 carbon atoms, such as ethanol or propanol; polyols, such as propylene glycol, sorbitol, glycerol, panthenol or polyethylene glycols and mixtures thereof.

When the composition of the invention is in the form of an emulsion, the composition may also optionally comprise a surfactant.

The composition may also comprise film-forming polymers such as polyurethanes, polyacrylic acid homo-or copolymers, polyesters, hydrocarbon-based resins and/or silicone resins. The polymer may be dissolved or dispersed in a cosmetically acceptable vehicle and optionally combined with a plasticizer.

The compositions of the present invention may also comprise an oil phase containing oil-soluble or oil-dispersible components that are liquid at room temperature (25 ℃) and/or substances that are oily or waxy at room temperature, such as waxes, semisolids, gums, and mixtures thereof. The oil phase may also contain an organic solvent.

Typically liquid at room temperature, suitable oily substances include: hydrocarbon-based oils of animal origin, such as perhydrosqualene; hydrocarbon-based vegetable oils, such as liquid triglycerides of C4-10 fatty acids, e.g. heptanoic acid or octanoic acid triglycerides, or oils, e.g. sunflower oil, corn oil, soybean oil, grapeseed oil, castor oil, avocado oil, octanoic/decanoic acid triglycerides, jojoba oil; linear or branched hydrocarbons of mineral or synthetic origin, such as liquid paraffin and its derivatives, vaseline; synthetic esters and ethers, in particular esters of fatty alcohols, such as isopropyl myristate, 2-ethylhexyl palmitate, 2-octyldodecyl stearate, isostearyl isostearate; hydroxylated esters, such as isostearyl lactate, octyl hydroxystearate, octyl dodecyl hydroxystearate, heptanoates, octanoates and decanoates of fatty alcohols; polyol esters such as propylene glycol dicaprylate, neopentyl glycol diheptanoate, diethylene glycol diisononanoate, and pentaerythritol esters; c12-26-containing fatty alcohols, such as octyldodecanol, 2-butyloctanol, 2-hexyldecanol, 2-undecylpentadecanol, oleyl alcohol; fluoro and/or fluorosilicone oils based in part on hydrocarbons, silicone oils, volatile or non-volatile linear or cyclic polymethylsiloxanes which are liquid or semi-solid at room temperature, such as cyclic polydimethylsiloxanes and polydimethylsiloxanes, optionally containing phenyl groups, such as phenyltrimethicones, silicones and mixtures thereof.

The composition of the present invention may further comprise any component commonly used in the cosmetic field. These components include preservatives, aqueous phase thickeners (extract biopolymers, synthetic polymers) and fatty phase thickeners, fragrances, hydrophilic and lipophilic active agents and mixtures thereof.

The compositions of the invention may also comprise an additional particulate phase, which may be a pigment and/or a pearlescent agent and/or a filler used in cosmetic compositions.

Pigments may be present in the composition, suitable inorganic pigments include titanium oxide, zirconium oxide and cerium oxide as well as zinc oxide, iron oxide and ferric blue; suitable organic pigments include barium, strontium, calcium and aluminum lakes and carbon black.

Pearling agents may be present in the composition, suitable pearling agents include mica coated with titanium oxide, iron oxide or natural pigments.

Fillers may be present in the composition, suitable fillers include talc, silica, zinc stearate, mica, kaolin, nylon powder, polyethylene powder, teflon, starch, boron nitride, copolymer microspheres, such as silicone resin microbeads.

The oil phase of the compositions of the present invention may comprise one or more waxes, gums or mixtures thereof. Waxes include hydrocarbon-based waxes, fluoro waxes, and/or silicone waxes, and may be derived from vegetable, mineral, animal, and/or synthetic sources. Suitable waxes include beeswax, carnauba wax, candelilla wax, paraffin wax, microcrystalline wax, ozokerite; synthetic waxes include polyethylene waxes, silicone waxes containing C16-45. Gums are generally polydimethylsiloxanes or sodium carboxymethylcellulose or extracts, and semisolid substances are generally hydrocarbon-based compounds, such as lanolin and its derivatives.

The compositions of the present invention may be formulated into any suitable product form. Such product forms include, but are not limited to, aerosol sprays, creams, lotions, solids, liquids, dispersions, foams, gels, lotions, mousses, ointments, powders, patches, pomades, solutions, hand pump sprays, sticks, masks and towelettes. The compositions of the present invention may be conveniently used to prepare or as cosmetic, dermatological or pharmaceutical topical products by various methods well known in the art.

The composition for external skin preparations of the present invention may include one or more of the following ingredients: anti-allergic agents, antimicrobial agents, antioxidants, chelating agents, colorant depigmenting agents, emollients, emulsifiers, exfoliants, film formers, fragrances, humectants, insect repellents, lubricants, pharmaceutically active agents, moisturizers, light stabilizers, preservatives, skin protectants, skin penetration enhancers, sunscreens, stabilizers, surfactants, thickeners, viscosity modifiers, vitamins, or any combination thereof.

Detailed Description

The invention is further illustrated below with reference to specific examples. It is to be understood, however, that these examples are illustrative only and are not to be construed as limiting the scope of the present invention. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the manufacturer. All percentages and parts are by weight unless otherwise indicated.

The sources of the raw materials used in the examples are as follows:

folium artemisiae argyi: purchased from Huayu pharmaceutical industry;

ethanol: purchased from national drug agents.

Example 1: preparation method of folium Artemisiae Argyi extract

100g of folium artemisiae argyi (commercially available) is decocted and extracted by 10 times of water and deionized water for 3 times, dregs of a decoction are removed to obtain a crude extract, the crude extract is concentrated to 200g, 4 times of ethanol is precipitated, the crude extract is filtered, and the filtrate is concentrated to the crude drug concentration of 0.5g/ml to obtain the folium artemisiae argyi extract.

Example 2: preparation method of folium Artemisiae Argyi extract

Extracting folium Artemisiae Argyi (commercially available) 100g with 10 times of water and deionized water for 2 times, removing residue to obtain crude extract, concentrating to 150g, precipitating with 3 times of ethanol, filtering, and concentrating the filtrate to crude drug concentration of 1 g/ml.

Example 3: preparation method of folium Artemisiae Argyi extract

100g of folium artemisiae argyi (commercially available) is obtained, 10 times of water deionized water is taken, microwave-assisted extraction is carried out for 2 times, dregs are removed to obtain crude extract, the crude extract is concentrated to 100g, 3 times of ethanol precipitation is carried out, filtration is carried out, and the filtrate is concentrated to the crude drug concentration of 1g/ml to obtain the traditional Chinese medicine preparation.

Example 4: preparation method of folium Artemisiae Argyi extract

Decocting folium Artemisiae Argyi (commercially available) 100g with 10 times of deionized water for 2 times, removing residue to obtain crude extractive solution, filtering with ceramic membrane with pore diameter of 50nm, collecting dialysate, and concentrating to crude drug concentration of 0.5 g/ml.

Example 5: epidermal cell moisturizing and/or barrier-associated gene expression based analysis

1. Test materials

1.1 cells: epidermal cell Hacat (Boxi Biotechnology Co., Ltd.)

1.2 reagent:

epidermal cell culture fluid KcGrowth (Boxi Biotechnology Co., Ltd.), PBS, MTT, DMSO, RNA extraction kit (Takara), reverse transcription kit (Takara), fluorescent dye (Takara)

1.3 Main Equipment

CO2 incubator, super clean bench, inverted microscope, micro-oscillator, enzyme-labeling instrument (BioTek), incubator, fluorescence quantitative PCR instrument (BioRad)

2. Sample preparation:

according to the experimental design, the folium artemisiae argyi extract of example 2 is prepared in different concentrations by diluting with culture solution, and the tested concentration range is 10% -0.078% (V/V).

3. The experimental method comprises the following steps:

MTT colorimetric method is adopted for detection, and concentration which has no influence on cell activity is selected for carrying out gene expression experiment.

Cells were seeded into 6-well plates at 37 ℃ CO₂Incubate overnight. The administration was carried out when the plating rate of the cells in the 6-well plate reached 40%. Experiment setup dosing group and control group, 0.313% (V/V) of the mugwort leaf extract of example 2 was added to the experimental group, and fresh culture medium was added to the control group at 37 deg.C and 5% CO₂The incubator continues to culture for 24 h. After the incubation culture is finished, the culture solution is discarded, 1mL of Trizol is added into each hole, and the lysed cells are collected after being blown. The RNA is extracted and the RNA is extracted,and carrying out reverse transcription for fluorescence quantitative PCR sample collection.

4. Data statistics and analysis:

the experimental results are expressed as "mean ± standard deviation" and analyzed with Microsoft Excel statistical software. Pairwise comparison by t test, P<0.05 indicated a significant difference. The real-time fluorescence quantitative result adopts 2-^△△CThe method carries out calculation.

5. The experimental results are as follows:

the results are shown in Table 1.

TABLE 1

By adopting 2-^△△CThe results were calculated and the significance was expressed as x when statistically analyzed by the T-Test method, with P-values <0.05 and P-values < 0.01.

As can be seen from fig. 1-2, the artemisia leaf extract in example 2 has a significant promoting effect on aquaporin 3 (p <0.05), and has a very significant promoting effect on lorin and filaggrin FLG (p < 0.01).

The mugwort leaf extract prepared in examples 1 to 4 was used for the preparation of skin external preparations. The skin external preparation is preferably a cosmetic composition such as a lotion, essence, cream, etc. The folium artemisiae argyi extract accounts for 0.0001-20% (w/w) of the external preparation for skin, preferably 0.001-10% (w/w) of the external preparation for skin, and more preferably 0.01-5% (w/w) of the external preparation for skin.

The following are examples of specific applications of the mugwort extract in skin external preparations, and formulations and preparation methods of these formulations. In the tables, "-" indicates no addition.

The mugwort extracts used in the following examples 6 to 16 were all the mugwort extracts prepared in examples 1 to 4, and the crude drug concentration thereof was 1 g/ml; the units of the components in examples 6-16 are weight percentages.

Example 6: preparation of face cream

Example 7: preparation of the emulsion

Example 8: preparation of jelly

Example 9: preparation of astringent

Example 10: preparation of essence

Example 11: preparation of facial mask

Example 12: preparation of eye cream

Example 13: preparation of an aerosol (cleaning foam)

Example 14: preparation of the spray

Example 15: preparation of shower gel

Example 16: preparation of facial cleanser

Claims

1. The application of the folium artemisiae argyi extract in skin moisturizing and/or skin barrier comprises the following extraction processes of: decocting, extracting, or a combination thereof.

2. Use according to claim 1, wherein the extraction solvent is selected from: water, ethanol, and combinations thereof.

3. The use of claim 1, wherein the extraction of the artemisia argyi extract further comprises alcohol precipitation, membrane filtration, or a combination thereof.

4. The use according to claim 1, wherein the weight ratio of the mugwort leaves to the extraction solvent in the extraction process is 1:10 or more.

5. The use according to claim 1, wherein the skin moisturization and/or skin barrier effect is achieved by improving the expression of a gene associated with moisturization and/or barrier efficacy.

6. The use of claim 5, wherein the genes associated with moisturization and/or barrier efficacy comprise aquaporin 3, loricrin, filaggrin, and combinations thereof.

7. The application of the folium artemisiae argyi extract in preparing the skin external preparation with the effects of moisturizing the skin and/or protecting the skin barrier comprises the following extraction processes of: decocting, extracting, or a combination thereof.

8. The use of claim 7, wherein the external skin agent is selected from the group consisting of: face cleaning lotion, cosmetic water, lotion, cream and facial mask.

9. The use of claim 7, wherein the external preparation for skin comprises 0.001 to 20% by weight of the extract of mugwort leaf.