CN111233544A - Microbial fermentation treatment method for excrement in high and cold areas - Google Patents
Microbial fermentation treatment method for excrement in high and cold areas Download PDFInfo
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Abstract
The invention provides a microbial fermentation treatment method for excrement in alpine regions, which comprises the following steps: (1) carrying out solid-liquid separation on the collected excrement by adopting solid-liquid dry-wet separation equipment; (2) transferring the excrement from which the liquid is separated into an excrement fermentation device; (3) piling up the excrement in the excrement fermentation device into a fermentation pile, (4) adding a cold-resistant composite strain agent, wherein 0.8-1.2kg of cold-resistant composite strain is added to each ton of excrement; (5) adding a nitrogen supplement into a fermentation pile according to the following proportion, wherein 2-3kg of the nitrogen supplement is added into each ton of excrement; (6) the fermentation can be completed after stacking for 25-45 days at an average temperature of 5-8 ℃, and the water content of the fermentation stack is kept at 55-65% in the stacking process; (7) adding active functional bacteria, wherein 0.7-1.2kg of functional bacteria agent is added to each ton of excrement; continuously stacking for 11-22 days; (8) crushing by adopting crushing; (9) and (5) performing subpackage. The invention is suitable for carrying out harmless treatment on the excrement in a region with lower temperature, and the obtained product can be used as an organic fertilizer.
Description
Technical Field
The invention belongs to the technical field of feces innocent treatment, and particularly relates to a microbial fermentation treatment method for feces in a high and cold area.
Background
The harmless excrement stacking method is mature, mainly performs fermentation and methane utilization, however, due to the climatic characteristics of low annual average temperature, large day-night temperature difference and the like in alpine regions, microorganisms in the regions are in a low-activity state for a long time, the fermented microorganisms grow and reproduce slowly, and the bacterial strain is not easy to survive in excrement according to the existing compost fermentation treatment method, so that the excrement cannot be fully fermented. A geographically severe cold is a climate characteristic, particularly cold climate zones due to high altitude or due to high altitude. The reason why people live at high altitude and in cold climate is that people mainly live in the troposphere atmosphere of the earth surface, and in the atmosphere, the change of air temperature and the altitude of the atmosphere are in a certain relationship, namely, the air temperature is about 0.6 ℃ when the altitude is increased by 100 meters on average. According to this reason, the higher the altitude, the colder the climate. For example, one of the most prominent natural geographical features in the Qinghai-Tibet plateau of China is severe chills.
The low annual average temperature in Qinghai-Tibet plateau constitutes the main characteristic of the climate. The average temperature of cocoa in the northwest of Tibet plateau is below 0 deg.C, and the isotherm coincides with the contour line to form a closed low-temperature center, which is the region of the Tibet plateau with the lowest temperature and is also the region of the northern hemisphere with the lowest temperature at the same latitude. The precipitation is little, and the regional difference is big. The plateau year precipitation is gradually reduced from 4000 mm above in the southeast of the Tibetan province to the cold lake in the Chauda basin, and the precipitation of the cold lake is only 17.5 mm. Taking baxika of the valley of the Bryonia delujin in Yalu as an example, the precipitation is extremely abundant, and the average annual precipitation reaches 4500 mm, which is 200 times of the minimum precipitation. The alpine pastoral area of the Qinghai-Tibet plateau has rich organic fertilizer resources, and an efficient utilization mode does not exist at present. The untreated cattle and sheep manure not only has low fertilizer efficiency, but also pollutes grasslands, and further causes serious non-point source pollution along with precipitation entering water bodies. Due to special natural geographical conditions and the current situation of social development, the livestock manure composting treatment in alpine pasturing areas has certain technical problems: the Qinghai-Tibet plateau has high altitude, low temperature, large temperature difference between day and night and difficult composting; the raw material collection is limited due to the complex terrain and scattered herdsmen; the shortage of labor resources in minority regions is not suitable for adopting a large-scale manual operation mode. Therefore, the composting mode which is suitable for small pastoral to rapidly compost and save labor cost has positive significance for grazing in alpine pastoral areas and effectively utilizing livestock manure.
Disclosure of Invention
In order to solve the problems of the prior art, the invention aims to provide a microbial fermentation treatment method for excrement in alpine regions, which can sufficiently ferment the excrement in a low-temperature environment for harmless treatment.
In order to achieve the purpose, the technical scheme of the invention is as follows:
the microbial fermentation treatment method of excrement in alpine regions comprises the following steps:
(1) carrying out solid-liquid separation on the collected excrement by adopting solid-liquid dry-wet separation equipment to remove partial moisture in the excrement;
(2) transferring the excrement from which the liquid is separated into an excrement fermentation device;
(3) the excrement in the excrement fermentation device is piled into fermentation piles, the weight of each fermentation pile is 20-30 tons, and the height of each fermentation pile is 2-3 meters;
(4) adding a cold-resistant composite strain agent into the fermentation pile according to the following proportion, and adding 0.8-1.2kg of cold-resistant composite strain into each ton of excrement;
(5) adding a nitrogen supplement into a fermentation pile according to the following proportion while adding zymophyte, wherein 2-3kg of nitrogen supplement is added into each ton of excrement;
(6) the fermentation pile added with the nitrogen supplement is stacked for 25 to 45 days at the average temperature of 5 to 8 ℃ to complete the fermentation, and the water content of the fermentation pile is kept between 55 and 65 percent in the stacking process;
(7) adding active functional bacteria in the fermented pile according to the following ratio, and adding 0.7-1.2kg of functional microbial inoculum to each ton of excrement; continuously stacking the fermentation pile added with the active functional bacteria for 11-22 days to obtain the feces harmless organic fertilizer;
(8) after fermentation in the excrement fermentation device is finished, crushing the harmless excrement organic fertilizer by adopting crushing;
(9) subpackaging the crushed harmless organic fertilizer of the excrement.
Wherein the cold-resistant composite strain agent consists of dry powder of yeast, brevibacterium, lactobacillus and bacillus.
Further, the yeast, the brevibacterium, the lactobacillus and the bacillus are mixed according to the following mass ratio: 10-15 parts of yeast, 35-40 parts of brevibacterium, 30-55 parts of lactobacillus and 40-50 parts of bacillus.
Mixing the cold-resistant composite strain with water according to the following mass ratio to obtain a cold-resistant composite strain base liquid, wherein the weight ratio of the cold-resistant composite strain dry powder to the water is 1: 20-25;
mixing the cold-resistant composite strain base liquid, the starch matrix and the urea according to the following mass ratio to obtain a mixed material, wherein the weight ratio of the cold-resistant composite strain base liquid to the starch matrix to the urea is 15-25: 45: 0.8 to 1.2, wherein the water content of the mixed material is 55 to 65 percent;
and then placing the mixed material in an environment with the temperature of 5-12 ℃ for natural propagation for 15-22 days, and stirring the mixed material once every two days to obtain the cold-resistant composite strain agent.
Wherein the starch substrate is corn flour.
Preferably, in the step (4), the hardiness complex strain agent is added into the fermentation piles, a vertical hole is punched downwards in the center of the top of each fermentation pile, and then the hardiness complex strain agent is added into the hole. The operation mode is very necessary and is a key step for improving the fermentation efficiency of the compost at the later stage; preferably, the punching depth is respectively 20-30cm from the top to the bottom, 3-5 times of adding are sequentially added at the depth of 20-30cm, and the added compound strain agent is preferably evenly divided according to the times of adding points; drilling and adding are carried out at positions 15-20 of the average distribution to form matrix type adding points, which are empirical values obtained by the inventor through years of experiments, and the effect is very good.
Further, in the step (5), the nitrogen supplement is urea.
In the step (6), in the stacking process of the fermentation pile, when the temperature of the center of the fermentation pile exceeds 65 ℃, the fermentation pile is turned over and then stacked again.
The active functional bacteria in the step (7) are bacillus subtilis.
In the actual production process, the method of the invention is more suitable for being matched with a ventilation pipe embedding and inserting mode rather than the traditional turning treatment. In addition, the use of the double-layer heat-insulating film is also a very important characteristic, and the following requirements are required: the manure pile and the double-layer heat preservation film are directly spaced by 0.3-0.5 m, and in addition, the distance between the two layers of films is also 0.5-1 m.
The method of the invention is different from the method in the prior art, the common method in the prior art is that the first layer of membrane is directly paved on the manure pile, and the second layer of membrane and the first layer of membrane have a space directly, the advantage is that the cost is lower, only a bracket is added between the two layers of membranes, but the inventor finds that the two layers of brackets and the two layers of air space can bring great benefit, only one investment is needed, the cost is saved in the subsequent replacement, and the method is very convenient when bacteria are added, and the method of the invention is to drill holes on the manure pile, so that the structure of the double-layer membrane is very convenient to use. In addition, the stack turning in the step 6 with the overtemperature is more convenient. However, as the manure piles belong to raw materials with stronger fluidity, the inventor builds a fence of about 1 meter by using hard plastic around, thereby being beneficial to the moulding of the manure piles and saving space.
Wherein, the manure heap 1 is put in the space that has the jube 4, and the region that the jube 4 can enclose is circular, square, rectangle, though according to the place, but preferred circular, and such structure is more stable, also is convenient for settle the heat preservation membrane, and wherein, inlayer heat preservation membrane 2 and outer heat preservation membrane 3 are placed respectively on the support. The heat preservation effect is very good, and mode efficiency than current double-deck heat preservation membrane increases a lot, is convenient for heat up, and the mode of the circulation of air of also being convenient for is cooled down, so the accuse temperature is easier, has very big effect to the improvement of compost effect.
The microbial fermentation treatment method for the excrement in the alpine region is suitable for performing harmless treatment on the excrement in a region with lower temperature, and the obtained product can be used as an organic fertilizer. Compared with the prior art, the invention has the beneficial effects that: the cold-resistant composite strain agent which is prepared by mixing saccharomycetes, brevibacterium, lactobacillus and bacillus according to a certain proportion can rapidly grow and reproduce under the high and cold condition, and can rapidly carry out biological fermentation on the excrement, thereby achieving the purpose of carrying out harmless treatment on the excrement in the area; the processing cost is low, and the period is short; after the excrement is subjected to harmless treatment, macromolecular substances in the excrement can be quickly decomposed into organic fertilizers which can be absorbed and utilized by plants.
Drawings
FIG. 1 shows the production of a manure pile under a double-layered insulating film under a pen; the manure pile 1 is placed in a space with the partition 4, the area which can be enclosed by the partition 4 is circular, square or rectangular, although the circular area is preferred according to the field, the structure is stable, and the heat-insulating film is convenient to place, wherein the inner heat-insulating film 2 and the outer heat-insulating film 3 are respectively placed on the bracket.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples, but the scope of the present invention is not limited thereto.
Example 1
The composting site was selected from the lower temple of Kangduoxiang, Zhunni, Calif. The southeast Tibetan autonomous state is one of ten Tibetan autonomous states in China, is located in the southwest part of Gansu province of China, is located in the northeast edge of the Qinghai-Tibet plateau and in the transition region of the west part of the loess plateau, and has wide interior grassland. The altitude is 2960 meters, the average temperature is 1.7 ℃, the frost-free period is short, the sunshine time is long, and the climate is a typical continental climate. Belonging to typical alpine pasturing areas. The experimental time is 6-10 months in 2019, the field is firstly cleaned of sundries before the experiment, the field is leveled and tamped, and double layers of 1.2 mm of waterproof cloth are laid for water-proof treatment, so that the infiltration of compost leachate is prevented. The whole field is isolated by nylon ropes and wooden piles, so that human and animal interference is prevented.
The organic fertilizer is prepared from Tibetan sheep manure, and is collected from a self-healing multi-country shepherd colony house, wherein the water content of the organic fertilizer is 14.2%, the organic matter content of the organic fertilizer is 62.2%, and the total nitrogen content of the organic fertilizer is 1.61%. The covering plastic film is 0.08 mm polyethylene shed film, and the vent pipe is made of high-quality PVC pipe with the diameter of 5 cm.
The temperature measurement was carried out using a commercially available 20-way temperature sensor. The sensor has the range of-40 to 150 ℃ and the resolution of 0.1 ℃. The temperature measurement adopts a mode of automatic recording by a sensor, different time intervals are adopted according to different stages, the temperature rise stage adopts recording for 1 time every 20 min, and the recording for 1 time after the high temperature stage is reached is carried out for 1 hour. 2 sensor probes are embedded in each stack body, and the central temperature of the stack body and the temperature 15 cm away from the surface layer are respectively measured. And the other two paths of temperature probes are arranged at the position 0.35 m away from the ground in the center of the composting site to measure the ambient temperature.
The microbial fermentation treatment method of excrement in alpine regions comprises the following steps:
(1) carrying out solid-liquid separation on the collected excrement by adopting solid-liquid dry-wet separation equipment to remove partial moisture in the excrement;
(2) transferring the excrement from which the liquid is separated into an excrement fermentation device;
(3) the excrement in the excrement fermentation device is piled up into fermentation piles, the weight of each fermentation pile is 25 tons, and the height of each fermentation pile is 2.5 meters;
(4) adding a cold-resistant composite strain agent into the fermentation pile according to the following proportion, wherein 1kg of cold-resistant composite strain is added into each ton of excrement;
the cold-resistant composite strain agent consists of dry powder of yeast, brevibacterium, lactobacillus and bacillus. The yeast, the brevibacterium, the lactobacillus and the bacillus are mixed according to the following mass ratio: 12 parts of yeast, 38 parts of brevibacterium, 52 parts of lactobacillus and 44 parts of bacillus.
Mixing the cold-resistant composite strain with water according to the following mass ratio to obtain a cold-resistant composite strain base liquid, wherein the weight ratio of the cold-resistant composite strain dry powder to the water is 1: 22; mixing the cold-resistant composite strain base liquid, the starch matrix and the urea according to the following mass ratio to obtain a mixed material, wherein the weight ratio of the cold-resistant composite strain base liquid to the starch matrix to the urea is 21: 45: 1.2, the water content of the mixed material is 55-65%; wherein the starch substrate is corn flour; and then placing the mixed material in an environment with the temperature of 5-12 ℃ for natural propagation for 20 days, and stirring the mixed material once every two days to obtain the cold-resistant composite strain agent.
Adding the cold-resistant composite strain agent into the fermentation piles, punching vertical holes downwards in the center of the top of each fermentation pile, and then adding the cold-resistant composite strain agent into the holes.
(5) Adding a nitrogen supplement into a fermentation pile according to the following proportion while adding zymophyte, wherein 2.6kg of nitrogen supplement is added into each ton of excrement; the nitrogen element supplement is urea.
(6) The fermentation pile added with the nitrogen supplement can be fermented after being stacked for 35 days at the average temperature of 5-8 ℃, and the water content of the fermentation pile is kept at 55-65% in the stacking process; in the stacking process of the fermentation pile, when the temperature of the center of the fermentation pile exceeds 65 ℃, the fermentation pile is turned over and then stacked again.
(7) Adding active functional bacteria into a fermentation pile after fermentation is finished according to the following proportion, and adding 1.2kg of functional bacteria agent into each ton of excrement; continuously stacking the fermentation pile added with the active functional bacteria for 18 days to obtain the harmless organic fertilizer for the excrement; the active functional bacteria are bacillus subtilis.
(8) After fermentation in the excrement fermentation device is finished, crushing the harmless excrement organic fertilizer by adopting crushing;
(9) subpackaging the crushed harmless organic fertilizer of the excrement.
Example 2
The microbial fermentation treatment method of excrement in alpine regions comprises the following steps:
(1) carrying out solid-liquid separation on the collected excrement by adopting solid-liquid dry-wet separation equipment to remove partial moisture in the excrement;
(2) transferring the excrement from which the liquid is separated into an excrement fermentation device;
(3) the excrement in the excrement fermentation device is piled up into fermentation piles, the weight of each fermentation pile is 20 tons, and the height of each fermentation pile is 2 meters;
(4) adding a cold-resistant composite strain agent into the fermentation pile according to the following proportion, and adding 0.8kg of cold-resistant composite strain into each ton of excrement;
the cold-resistant composite strain agent consists of dry powder of yeast, brevibacterium, lactobacillus and bacillus. The yeast, the brevibacterium, the lactobacillus and the bacillus are mixed according to the following mass ratio: 14 parts of yeast, 38 parts of brevibacterium, 52 parts of lactobacillus and 41 parts of bacillus.
Mixing the cold-resistant composite strain with water according to the following mass ratio to obtain a cold-resistant composite strain base liquid, wherein the weight ratio of the cold-resistant composite strain dry powder to the water is 1: 24; mixing the cold-resistant composite strain base liquid, the starch matrix and the urea according to the following mass ratio to obtain a mixed material, wherein the weight ratio of the cold-resistant composite strain base liquid to the starch matrix to the urea is 17: 45: 1, the water content of the mixed material is 55-65%; wherein the starch substrate is corn flour; and then placing the mixed material in an environment with the temperature of 5-12 ℃ for natural propagation for 18 days, and stirring the mixed material once every two days to obtain the cold-resistant composite strain agent.
Adding the cold-resistant composite strain agent into the fermentation piles, punching vertical holes downwards in the center of the top of each fermentation pile, and then adding the cold-resistant composite strain agent into the holes.
(5) Adding a nitrogen supplement into a fermentation pile according to the following proportion while adding zymophyte, wherein 2-3kg of nitrogen supplement is added into each ton of excrement; the nitrogen element supplement is urea.
(6) The fermentation pile added with the nitrogen supplement can be fermented after being stacked for 34 days at the average temperature of 5-8 ℃, and the water content of the fermentation pile is kept at 55-65% in the stacking process; in the stacking process of the fermentation pile, when the temperature of the center of the fermentation pile exceeds 65 ℃, the fermentation pile is turned over and then stacked again.
(7) Adding active functional bacteria into a fermentation pile after fermentation is finished according to the following proportion, and adding 1.2kg of functional bacteria agent into each ton of excrement; continuously stacking the fermentation pile added with the active functional bacteria for 15 days to obtain the harmless organic fertilizer for the excrement; the active functional bacteria are bacillus subtilis.
(8) After fermentation in the excrement fermentation device is finished, crushing the harmless excrement organic fertilizer by adopting crushing;
(9) subpackaging the crushed harmless organic fertilizer of the excrement.
Example 3
The microbial fermentation treatment method of excrement in alpine regions comprises the following steps:
(1) carrying out solid-liquid separation on the collected excrement by adopting solid-liquid dry-wet separation equipment to remove partial moisture in the excrement;
(2) transferring the excrement from which the liquid is separated into an excrement fermentation device;
(3) the excrement in the excrement fermentation device is piled up into fermentation piles, the weight of each fermentation pile is 30 tons, and the height of each fermentation pile is 3 meters;
(4) adding a cold-resistant composite strain agent into the fermentation pile according to the following proportion, wherein 1.2kg of cold-resistant composite strain is added into each ton of excrement;
the cold-resistant composite strain agent consists of dry powder of yeast, brevibacterium, lactobacillus and bacillus. The yeast, the brevibacterium, the lactobacillus and the bacillus are mixed according to the following mass ratio: 13 parts of yeast, 37 parts of brevibacterium, 53 parts of lactobacillus and 43 parts of bacillus.
Mixing the cold-resistant composite strain with water according to the following mass ratio to obtain a cold-resistant composite strain base liquid, wherein the weight ratio of the cold-resistant composite strain dry powder to the water is 1: 23; the cold-resistant composite strain base liquid, the starch matrix and the urea are mixed according to the following mass ratio to obtain a mixed material, wherein the weight ratio of the cold-resistant composite strain base liquid to the starch matrix to the urea is 23: 45: 1.1, the water content of the mixed material is 55-65%; wherein the starch substrate is corn flour; and then placing the mixed material in an environment with the temperature of 5-12 ℃ for natural propagation for 20 days, and stirring the mixed material once every two days to obtain the cold-resistant composite strain agent.
Adding the cold-resistant composite strain agent into the fermentation piles, punching vertical holes downwards in the center of the top of each fermentation pile, and then adding the cold-resistant composite strain agent into the holes.
(5) Adding a nitrogen supplement into a fermentation pile according to the following proportion while adding zymophyte, wherein 3kg of the nitrogen supplement is added into each ton of excrement; the nitrogen element supplement is urea.
(6) The fermentation pile added with the nitrogen supplement can be fermented after being piled for 33 days at the average temperature of 5-8 ℃, and the water content of the fermentation pile is kept between 55 and 65 percent in the piling process; in the stacking process of the fermentation pile, when the temperature of the center of the fermentation pile exceeds 65 ℃, the fermentation pile is turned over and then stacked again.
(7) Adding active functional bacteria in a fermented pile according to the following ratio, and adding 1kg of functional microbial inoculum to each ton of excrement; continuously stacking the fermentation pile added with the active functional bacteria for 13 days to obtain the harmless organic fertilizer for the excrement; the active functional bacteria are bacillus subtilis.
(8) After fermentation in the excrement fermentation device is finished, crushing the harmless excrement organic fertilizer by adopting crushing;
(9) subpackaging the crushed harmless organic fertilizer of the excrement.
The method has good effect, and the compost product quality is characterized in that the change of the organic matter content of the compost is 42.0-52.2%, the total nitrogen content is 1.31-1.42%, and the organic matter content reaches the national organic fertilizer industry standard (NY 525-2012). The total nitrogen loss rate of the compost is 17.2% -41.7%, and the organic matter loss rate is changed to be 28.2% -47.2%.
The final evaluation criteria of good and bad composting effect are preferably short composting time, high nutrient content and high temperature equalization of a compost body. In combination with the above analysis, it can be seen that the aeration pattern has a dominant effect on the overall composting process and the final fertilizer efficiency.
In addition, according to the discovery of the inventor in the actual operation process, the treatment of burying and inserting the vent pipe is superior to the pile turning treatment in the aspects of increasing the pile corrosion temperature and shortening the pile corrosion period, and the organic matter content and the total nitrogen content are also obviously higher than the pile turning treatment, which shows that the whole pipe inserting treatment is superior to the pile turning treatment. Although individual indicators show significant beneficial effects, the covering cannot determine the most critical stack temperature, and therefore can only be an effective aid to increase the degree of stack corrosion. The addition of phosphorus accelerates the composting process, but the lifting effect is not as good as that of burying and inserting the vent pipe, and the adverse effect of aggravation of nutrient loss exists. So the optimal combination selected last according to the selection of the optimal level of the orthogonal test is: burying and inserting a breather pipe, covering a small-spacing double-layer film, and adding zymophyte for the second time.
Claims (9)
1. The microbial fermentation treatment method for the excrement in the alpine region is characterized by comprising the following steps of:
(1) carrying out solid-liquid separation on the collected excrement by adopting solid-liquid dry-wet separation equipment to remove partial moisture in the excrement;
(2) transferring the excrement from which the liquid is separated into an excrement fermentation device;
(3) the excrement in the excrement fermentation device is piled into fermentation piles, the weight of each fermentation pile is 20-30 tons, and the height of each fermentation pile is 2-3 meters;
(4) adding a cold-resistant composite strain agent into the fermentation pile according to the following proportion, and adding 0.8-1.2kg of cold-resistant composite strain into each ton of excrement;
(5) adding a nitrogen supplement into a fermentation pile according to the following proportion while adding zymophyte, wherein 2-3kg of nitrogen supplement is added into each ton of excrement;
(6) the fermentation pile added with the nitrogen supplement is stacked for 25 to 45 days at the average temperature of 5 to 8 ℃ to complete the fermentation, and the water content of the fermentation pile is kept between 55 and 65 percent in the stacking process;
(7) adding active functional bacteria in the fermented pile according to the following ratio, and adding 0.7-1.2kg of functional microbial inoculum to each ton of excrement; continuously stacking the fermentation pile added with the active functional bacteria for 11-22 days to obtain the feces harmless organic fertilizer;
(8) after fermentation in the excrement fermentation device is finished, crushing the harmless excrement organic fertilizer by adopting crushing;
(9) subpackaging the crushed harmless organic fertilizer of the excrement.
2. The microbial fermentation treatment method of feces in alpine regions according to claim 1, wherein the cold-resistant complex strain agent is composed of dry powder of yeast, brevibacterium, lactobacillus and bacillus.
3. The microbial fermentation treatment method for the excrement in the alpine region according to claim 2, wherein the yeast, the brevibacterium, the lactobacillus and the bacillus are mixed according to the following mass ratio: 10-15 parts of yeast, 35-40 parts of brevibacterium, 30-55 parts of lactobacillus and 40-50 parts of bacillus.
4. The microbial fermentation method of feces in alpine regions according to claim 2 or 3,
mixing the cold-resistant composite strain with water according to the following mass ratio to obtain a cold-resistant composite strain base liquid, wherein the weight ratio of the cold-resistant composite strain dry powder to the water is 1: 20-25;
mixing the cold-resistant composite strain base liquid, the starch matrix and the urea according to the following mass ratio to obtain a mixed material, wherein the weight ratio of the cold-resistant composite strain base liquid to the starch matrix to the urea is 15-25: 45: 0.8 to 1.2, wherein the water content of the mixed material is 55 to 65 percent;
and then placing the mixed material in an environment with the temperature of 5-12 ℃ for natural propagation for 15-22 days, and stirring the mixed material once every two days to obtain the cold-resistant composite strain agent.
5. The microbial fermentation method for treating excrement in alpine regions according to claim 4, wherein the starch substrate is corn flour.
6. The microbial fermentation treatment method for excrement in alpine regions according to claim 1, wherein in the step (4), the cold-resistant compound strain agent is added into the fermentation piles, a vertical hole is punched downwards in the center of the top of each fermentation pile, and then the cold-resistant compound strain agent is added into the holes.
7. The microbial fermentation method for treating feces in alpine regions according to claim 1, wherein in the step (5), the nitrogen supplement is urea.
8. The microbial fermentation treatment method for excrement in alpine regions according to claim 1, wherein in the step (6), the fermentation pile is turned over and stacked again after the temperature of the center of the fermentation pile exceeds 65 ℃ in the stacking process.
9. The microbial fermentation treatment method for excrement of alpine regions according to claim 1, wherein the active functional bacteria in the step (7) is bacillus subtilis.
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