CN111228405B

CN111228405B - A Chinese medicinal composition for treating gastrointestinal diseases, and its preparation method

Info

Publication number: CN111228405B
Application number: CN202010243628.XA
Authority: CN
Inventors: 张京平; 黄万义
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-03-31
Filing date: 2020-03-31
Publication date: 2022-08-23
Anticipated expiration: 2040-03-31
Also published as: CN111228405A

Abstract

The invention discloses a traditional Chinese medicine composition for treating gastrointestinal diseases and a preparation method thereof, and the traditional Chinese medicine composition comprises the following raw materials: cuttlebone, astragalus root, white atractylodes rhizome, bletilla, thunberg fritillary bulb, catechu and bitter orange; by utilizing the treatment principle of the traditional Chinese medicine, the diarrhea due to spleen deficiency is thoroughly cured, the recurrence rate after stopping taking the medicine is obviously reduced, the moderate and chronic enteritis, the intestinal absorption dysfunction, the irritable bowel syndrome, the ulcerative colitis and the like can be effectively cured, and the diarrhea due to spleen deficiency of children can be effectively cured.

Description

A Chinese medicinal composition for treating gastrointestinal diseases, and its preparation method

Technical Field

The invention relates to the technical field of preparation of gastrointestinal disease medicines, in particular to a medicine for treating gastrointestinal diseases and a preparation method thereof.

Background

In modern medicine, chronic enteritis, intestinal absorption dysfunction, irritable bowel syndrome, ulcerative colitis and the like are mainly manifested by diarrhea, and have long course of disease and difficult treatment. Clinically, diarrhea due to spleen deficiency is characterized by loose stool, diarrhea, anorexia, abdominal distension, mental fatigue, hypodynamia, etc., and its basic pathogenesis is spleen deficiency with excessive dampness, spleen deficiency with failure to ascend clear, water retention as dampness, and food retention.

Western medicines are usually adopted for treatment in clinic, and although the medicine has a certain treatment effect in a short period, the recurrence rate is high after the medicine is stopped. In addition, the western medicines need to pay attention to the safety of the medicines after long-term administration, for example, animal experiments show that gastric carcinoid can be generated after long-term administration of omeprazole; the colloidal bismuth salt has been determined to have high cytotoxicity and neurotoxicity, is easy to cause encephalopathy, nephropathy, hepatopathy, arthralgia and the like, and is not suitable for long-term administration. Therefore, from the holistic concept of the traditional Chinese medicine, the characteristics of the traditional Chinese medicine are exerted by adopting a multi-target point and all-round formula principle, and the development of the traditional Chinese medicine preparation with high curative effect and low recurrence rate is necessary.

Therefore, the problem to be solved by the technical personnel in the field is to provide a traditional Chinese medicine composition for treating ulcerative colitis and a preparation method thereof.

Disclosure of Invention

In view of the above, the invention provides a traditional Chinese medicine composition for treating ulcerative colitis and a preparation method thereof, which adopts the traditional Chinese medicine treatment principle to thoroughly cure diarrhea due to spleen deficiency, obviously reduce the recurrence rate after stopping taking medicine, and effectively cure moderate chronic enteritis, intestinal absorption dysfunction, irritable bowel syndrome, ulcerative colitis and the like.

In order to achieve the purpose, the invention adopts the following technical scheme:

the invention provides a traditional Chinese medicine composition for treating gastrointestinal diseases, which adopts the following traditional Chinese medicine raw materials: cuttlebone, astragalus root, white atractylodes rhizome, bletilla tuber, thunberg fritillary bulb, catechu and bitter orange.

The traditional Chinese medicine composition for treating gastrointestinal diseases provided by the invention has the main functions: has effects of invigorating qi, invigorating spleen, relieving diarrhea, relieving hyperacidity, healing sore, and promoting granulation, and can be used for treating diarrhea, chronic diarrhea, mucus and blood stool due to weakness of spleen and stomach, incoordination between liver and stomach, abdominal pain, mucus and blood stool, gastrointestinal mucosa congestion, edema, ulcer hemorrhage, anorexia, listlessness, speaking laziness, and gastric acid. The traditional Chinese medicine composition provided by the invention has the effects of relieving hyperacidity and pain, invigorating spleen and relieving diarrhea, and astringing to stop bleeding, can promote ulcer healing, and can be clinically used for treating peptic ulcer bleeding and ulcerative colitis; and has effects of invigorating spleen and relieving oppression, and can be used for treating dyspepsia, chronic gastritis, chronic enteritis, etc., and can be used for treating gastrointestinal diseases such as acute and chronic gastritis, gastric and duodenal ulcer, gastrokinetic neurosis, etc., and can effectively treat infantile diarrhea due to spleen deficiency.

Preferably, the composition specifically comprises the following components in parts by weight: 70-100 parts of cuttlebone, 70-100 parts of astragalus, 60-100 parts of bighead atractylodes rhizome, 40-90 parts of bletilla, 50-100 parts of thunberg fritillary bulb, 40-100 parts of bitter orange and 40-100 parts of catechu.

Wherein, the rhizoma Atractylodis Macrocephalae is parched rhizoma Atractylodis Macrocephalae, and the fructus Aurantii is parched fructus Aurantii.

The invention also provides a preparation method of the traditional Chinese medicine composition for treating gastrointestinal diseases, which comprises the following steps:

(1) weighing the raw materials according to the traditional Chinese medicine composition for treating the gastrointestinal diseases for later use;

(2) cleaning the raw materials, drying, pulverizing, sieving, and mixing to obtain mixed medicinal powder;

(3) and preparing the mixed medicinal powder into powder or encapsulating, and sterilizing to obtain the traditional Chinese medicine composition for treating the gastrointestinal diseases.

Preferably, the sieving in the step (2) adopts a 80-200 mesh sieve.

Preferably, each capsule in the step (3) is filled with 0.5g of mixed medicinal powder.

According to the technical scheme, compared with the prior art, the traditional Chinese medicine composition for treating ulcerative colitis and the preparation method thereof have the following beneficial effects:

(1) the invention adopts the traditional Chinese medicine treatment principle, thoroughly cures the diarrhea due to spleen deficiency, and obviously reduces the recurrence rate after stopping taking medicine;

(2) the traditional Chinese medicine composition provided by the invention has the effects of relieving hyperacidity and pain, invigorating spleen and relieving diarrhea, and astringing and stopping bleeding, and can promote ulcer healing; it also has effects in invigorating spleen and relieving oppression, and can be used for treating dyspepsia, chronic gastritis, chronic enteritis, etc.; can effectively cure chronic enteritis, intestinal absorption dysfunction, irritable bowel syndrome, ulcerative colitis and the like, and can effectively cure infantile diarrhea due to spleen deficiency.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.

The embodiment of the invention discloses a traditional Chinese medicine composition for treating gastrointestinal diseases, which specifically comprises the following traditional Chinese medicine raw materials in parts by weight: 60-100 parts of cuttlebone, 60-100 parts of astragalus mongholicus, 40-100 parts of bighead atractylodes rhizome (fried), 40-100 parts of bletilla, 50-100 parts of thunberg fritillary bulb, 40-100 parts of fructus aurantii (fried) and 40-100 parts of catechu.

The invention also provides a preparation method of the traditional Chinese medicine composition for treating gastrointestinal diseases, which is characterized by comprising the following steps:

(2) cleaning, drying and crushing the raw materials, sieving the raw materials by a sieve of 80 to 200 meshes, and uniformly mixing the raw materials to obtain mixed medicinal powder;

(3) and (3) preparing the mixed medicinal powder into powder or encapsulating, and sterilizing to obtain the traditional Chinese medicine composition for treating the gastrointestinal diseases. Wherein each capsule is filled with 0.5g of mixed medicinal powder.

Example 1

The embodiment 1 of the invention discloses a traditional Chinese medicine composition for treating gastrointestinal diseases, which is prepared from the following traditional Chinese medicine raw materials in parts by weight: 100g of cuttlebone, 100g of astragalus, 100g of white atractylodes rhizome (fried), 100g of common bletilla pseudobulb, 100g of thunberg fritillary bulb, 100g of bitter orange (fried) and 100g of catechu.

The embodiment 1 of the invention also provides a preparation method of the traditional Chinese medicine composition for treating gastrointestinal diseases, which is characterized by comprising the following steps:

(3) encapsulating the mixed medicinal powder, and sterilizing to obtain the Chinese medicinal composition for treating gastrointestinal diseases. Wherein each capsule is filled with 0.5g of mixed medicinal powder.

Example 2

The embodiment 2 of the invention discloses a traditional Chinese medicine composition for treating gastrointestinal diseases, which is prepared from the following traditional Chinese medicine raw materials in parts by weight: 60g of cuttlebone, 60g of astragalus, 40g of white atractylodes rhizome (fried), 40g of bletilla, 50g of thunberg fritillary bulb, 40g of bitter orange (fried) and 40g of catechu.

The embodiment 2 of the invention also provides a preparation method of the traditional Chinese medicine composition for treating gastrointestinal diseases, which is characterized by comprising the following steps:

(2) cleaning, drying and crushing the raw materials, sieving the raw materials by a sieve of 80 to 200 meshes, crushing and uniformly mixing the raw materials to obtain mixed medicinal powder;

Example 3

The embodiment 3 of the invention discloses a traditional Chinese medicine composition for treating gastrointestinal diseases, which is prepared from the following traditional Chinese medicine raw materials in parts by weight: 100g of cuttlebone, 100g of astragalus, 100g of white atractylodes rhizome (fried), 40g of bletilla, 50g of thunberg fritillary bulb, 40g of bitter orange (fried) and 40g of catechu.

The embodiment 3 of the invention also provides a preparation method of the traditional Chinese medicine composition for treating gastrointestinal diseases, which is characterized by comprising the following steps:

(2) cleaning, drying and crushing the raw materials, sieving the raw materials by a sieve with 80 to 200 meshes, and uniformly mixing to obtain mixed medicinal powder;

Example 4

The embodiment 4 of the invention discloses a traditional Chinese medicine composition for treating gastrointestinal diseases, which is prepared from the following traditional Chinese medicine raw materials in parts by weight: 60g of cuttlebone, 60g of astragalus, 100g of white atractylodes rhizome (fried), 100g of bletilla, 100g of thunberg fritillary bulb, 90g of bitter orange (fried) and 90g of catechu.

The embodiment 4 of the invention also provides a preparation method of the traditional Chinese medicine composition for treating gastrointestinal diseases, which is characterized by comprising the following steps:

(3) and (4) preparing the mixed medicinal powder into powder, and sterilizing to obtain the traditional Chinese medicine composition for treating the gastrointestinal diseases.

Effect verification

Detection of antidiarrheal effect

1. Apparatus and materials

(1) An instrument MP120-1 type electronic balance (Wuhan automated instrument factory), an AE200 type electronic analytical balance (Mettler-Toledo instruments, Switzerland), and an HH-S constant temperature water bath (Jiangsu medical instrument factory).

(2) The white mice of Kunming species of animals, which are half male and female, have the body weight of 23 +/-2 g and 130 mice, are provided by the experimental animal center of Shanxi medical university and are fed for 3 days without adverse reaction and then are brought into the experiment.

1.3 reagent

Preparing a rhubarb water decoction: weighing 200g of radix et rhizoma Rhei (purchased from Shanxi province medicinal materials company, identified as genuine by the national institute of Chinese medicine identification and research laboratory cattle Yanzhen teacher), adding 6 times of water, soaking for 20min, decocting for 2 times, each time for 15min, mixing decoctions, filtering, and concentrating to 200ml (each 1ml is equivalent to 1g of crude drug).

The traditional Chinese medicine composition for treating gastrointestinal diseases prepared by the methods in the embodiments 1 to 4 are respectively prepared into suspensions with the medicine powder concentrations of 0.156g/mL (small dose) and 0.312g/mL (large dose) by using distilled water, wherein the clinical dosage is 2-4 granules/time and 3 times/day.

The ginseng, poria cocos and bighead atractylodes rhizome powder is prepared by Shanxi Huakang pharmaceutical industry Co., Ltd, with the batch number of 20070202, the clinical dosage of 6-9 g/time and 2-3 times/day, and is prepared into suspension containing 0.156g of medicinal powder per ml by using distilled water.

Yimengting is produced by Xian Yang Sen pharmaceutical Co., Ltd, batch No. 070227605, the adult initial dosage is 2 granules, the maximum dosage per day is not more than 8 granules, and distilled water is used for preparing suspension containing 0.042g of medicinal powder per ml, thus obtaining the Yimengting.

Preparation of activated carbon suspension: activated carbon powder was purchased from bodi chemical ltd, Tianjin, lot No. 2006123034. Weighing Arabic gum 10g, adding water to 75ml, boiling until the solution is transparent, weighing activated carbon powder 6g, adding into the above solution, boiling for 3 times, cooling, and adding water to 100 ml.

2. Experimental methods and results

(1) Molding machine

Mice were randomly divided into 10 normal control groups and 120 model groups. The mice of the model building group are fed with 0.5ml of rhubarb water decoction, and the normal control group is fed with the same volume of physiological saline 1 time a day and continuously infused with stomach for 7 days. The symptoms comprise weight loss, loose stool, dehydration, anorexia, emaciation, no limb contraction, dry hair, intolerance of cold, low body temperature and the like. After the molding is successful, the molding groups are randomly divided into 12 groups, namely a model group, a ginseng, poria, rhizoma atractylodis macrocephalae powder group, an easy-to-Mongolian group, an example 1 small-dose group, an example 1 large-dose group, an example 2 small-dose group, an example 2 large-dose group, an example 3 small-dose group, an example 3 large-dose group, an example 4 small-dose group and an example 4 large-dose group, wherein each group comprises 10 animals.

(2) Administration of drugs

Starting on day 8, in the morning: normal control group continuously gives 0.5ml of physiological saline for each, and molding group mice give 0.5ml of rhubarb water decoction for each; in the afternoon: the normal control group and the model group are respectively administered with corresponding suspension of 0.5ml each for 7 days by intragastric administration of normal saline, Shenling Baizhu san group, Yimengting group, small dose group and large dose group.

(3) Detection

Determination of wet manure number: after the administration of the drug by intragastric administration on day 6, each mouse was placed in a bell jar covered with filter paper, the filter paper was changed every hour, the continuous observation was carried out for 7 hours, the number of mouse stools on the filter paper was recorded, and the degree of diarrhea was represented by the number of wet stools. The results are shown in Table 1.

Determination of small intestine carbon powder propulsion rate: on day 7, fasting was performed for 12 hours, and then the mice were each administered with a liquid medicine containing activated carbon. After 20 minutes of administration, the cervical vertebrae were removed and sacrificed, the abdominal cavity was opened to separate mesentery, the intestinal canal from the pylorus to the ileocecal portion was cut and laid on a glass plate without traction, the total length of the small intestine and the length from the pylorus to the front edge of the charcoal powder (the advancing distance of the charcoal powder in the intestine) were measured, and the advancing percentage of the charcoal powder for each group of animals was calculated by the following formula. The results are shown in Table 2.

(4) The statistical analysis data calculation and statistical analysis are processed by using SPSS13.0 software. Data to

Indicated, the group comparisons were performed using the t-test.

TABLE 1 stool count for mice with diarrhea caused by rhubarb

Note: comparing with normal control group ¹⁾ P is less than 0.05, compared with the model group ²⁾ P is less than 0.05, and is compared with the powder of Ginseng, Poria and Atractylodis rhizoma ³⁾ P is less than 0.05, compared with the easy-to-Monte group ⁴⁾ P＜0.05

TABLE 2 carbon powder propulsion rate in the intestine of mice

(5) Analysis of results

From the results in table 1, it can be known that the number of wet feces of the mice in the small dose group is significantly different from that in the normal control group, the model group, the ginseng, poria, bighead atractylodes rhizome and easy Mongolian medicine group, which indicates that the traditional Chinese medicine composition prepared by the invention has a certain antidiarrheal effect on diarrhea caused by rhubarb when used in a small dose; the significant difference between the large-dose group and the normal control group, the model group and the ginseng, poria, and bighead atractylodes rhizome powder group indicates that the large-dose use has the effect of relieving diarrhea caused by rhubarb, and the action effect is close to that of easy-to-Mongolian medicine (P is more than 0.05).

As is evident from the results in Table 2, the intestinal transit rates of the treated groups were significantly different from those of the normal control group and the model group (P < 0.05). The ginseng, poria cocos and bighead atractylodes rhizome powder group and the Yimanting group have obvious difference among treatment groups, and the effect of the ginseng, poria cocos and bighead atractylodes rhizome powder is superior to that of the Yimanting group; compared with the Shenling Baizhu powder group and the Yimanting group, the small-dose group has obvious difference (P is less than 0.05, which indicates that the effect of the small-dose intestinal Wubei Changwei Ping capsule on the propulsion rate of the small intestinal charcoal powder of the diarrhea mice is not as good as that of the Shenling Baizhu powder and the Yimanting group, and compared with the Shenling Baizhu powder group and the Yimanting group, the large-dose group has no obvious difference (P is more than 0.05), which indicates that the effect of the large-dose Wubei Changwei Ping capsule on the propulsion rate of the small intestinal charcoal powder is close to that of the Shenling Baizhu powder and the Yimanting.

The spleen governs transportation, transformation and absorption, and the healthy function provides nutrients for transforming and generating essence, qi, blood and body fluids, so as to fully nourish the viscera and organs of the whole body, thereby ensuring the normal operation of various physiological functions of the body. The rhubarb has bitter and cold properties and strong purgation effect, can injure primordial qi and depress spleen and stomach after long-term use, and affects the absorption of food essence, and the rhubarb causes a spleen deficiency diarrhea model of mice with large dose of rhubarb, and the diarrhea model after stomach filling for 2-3 days causes the increase of excrement, soft stool, loose stool and anus pollution, and the symptoms become more severe along with the continuation of molding, and are manifested as loose stool, increased times and even mucus and blood stool. The modeling animal performance is similar to that of the spleen deficiency diarrhea of human bodies, the characteristics of the spleen deficiency diarrhea in traditional Chinese medicine are met, statistics shows that the wet manure number and the small intestine carbon powder propulsion rate of mice in the spleen deficiency diarrhea model group are obviously higher than those of a normal control group, and the modeling is successful.

The test result of the wet feces number of mice shows that the large dose of the medicine has the effect similar to that of the easy Mongolian medicine, but is not as good as that of the Shenling Baizhu powder. Small intestine charcoal powder propulsion rate test, the large dose effect of the product is similar to that of Shenling Baizhu powder and Yimenting powder.

Second, acute toxicity test detection

1. Material

(1) The weight of the Kunming breed white mouse is 23 +/-2 g, and 80 mice are respectively half male and female, provided by the experimental animal center of Shanxi medical university, and can be included in the experiment after being fed for 3 days without adverse reaction.

(2) Instrument MP120-1 type electronic balance (Wuhan automated instrument factory), AE200 type electronic analytical balance (Mettler-Toledo instruments, Switzerland)

(3) The traditional Chinese medicine composition capsules for treating gastrointestinal diseases, prepared in the medicine embodiments 1-4, are prepared into suspension with the maximum concentration by using distilled water, wherein the clinical dosage is 2-4 granules per time and 3 times per day.

2. The method comprises gavaging the animal with maximum concentration (0.429g/ml), maximum volume (0.4 ml per 10g body weight) for 2 times every 6 hr, and observing the appearance, behavior, activity, appetite, stool and urine, color, fur, etc. of the animal after administration. After continuous observation for 7 days, the mice were sacrificed by dislocation on day 8, dissected, and their major organs such as heart, liver, spleen, lung, and kidney were visually observed.

3. Observing the appearance, behavior, food intake and excretion of the animals in the experimental process of results; the patient was sacrificed on day 8 and examined by dissection, and the results of the examination of the major organs such as heart, liver, spleen, lung and kidney are shown in Table 3 below.

TABLE 3

The daily dosage of the mouse is 400 times of that of an adult, and no obvious acute toxicity reaction is seen, which indicates that the traditional Chinese medicine composition prepared by the invention has very low toxicity.

The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A traditional Chinese medicine composition for treating gastrointestinal diseases is characterized in that the adopted traditional Chinese medicine raw materials comprise the following components in parts by weight: 60-100 parts of cuttlebone, 60-100 parts of astragalus, 40-100 parts of bighead atractylodes rhizome, 40-100 parts of bletilla, 50-100 parts of thunberg fritillary bulb, 40-100 parts of bitter orange and 40-100 parts of catechu;

the preparation method of the traditional Chinese medicine composition for treating the gastrointestinal diseases specifically comprises the following steps:

(1) the traditional Chinese medicine composition for treating the gastrointestinal diseases is prepared by weighing the raw materials for later use;

(2) cleaning the raw materials, drying, pulverizing, sieving, and mixing to obtain mixed medicinal powder; the sieving adopts a 80-200 mesh sieve;

(3) making the mixed medicinal powder into powder or encapsulating, and sterilizing to obtain Chinese medicinal composition for treating gastrointestinal diseases; each capsule is filled with 0.5g of mixed medicinal powder.