CN111220683A - Method for real-time online monitoring of exhaled propofol - Google Patents
Method for real-time online monitoring of exhaled propofol Download PDFInfo
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- CN111220683A CN111220683A CN201811411820.4A CN201811411820A CN111220683A CN 111220683 A CN111220683 A CN 111220683A CN 201811411820 A CN201811411820 A CN 201811411820A CN 111220683 A CN111220683 A CN 111220683A
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- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/622—Ion mobility spectrometry
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Abstract
The invention discloses an ion mobility spectrometry detection method for real-time online monitoring of exhaled propofol, which adopts a sampling pump controlled by a flow meter to perform negative pressure sampling to realize real-time online sampling of exhaled propofol, samples a vacuum ultraviolet lamp photoionization source which can be respectively used for detecting exhaled propofol in a positive or negative ion mode, places three vacuum ultraviolet lamps at the upper side, the lower side and the left end of a reaction zone, and detects exhaled propofol under high humidity, so that the real-time ionization efficiency of propofol is improved, and the sensitivity of exhaled propofol real-time detection is improved.
Description
Technical Field
The invention relates to a real-time online monitoring method of exhaled propofol developed based on an ion mobility spectrometry photoionization technology.
Background
Propofol is a commonly used intravenous anesthetic, and studies have shown that the concentration of propofol in exhaled breath of a subject is well correlated with the concentration of propofol in blood of the subject, so that on-line analysis of exhaled breath can provide important anesthetic information for medical staff. In addition, propofol molecules in blood can appear in exhaled breath through metabolic processes such as blood circulation, cardiopulmonary exchange and the like, so that when the blood circulation and the cardiopulmonary function of a subject change, the change rule of propofol in exhaled breath is likely to change, and a real-time online monitoring means is essential for accurately and quickly capturing clinical information.
Currently, various mass spectrometry techniques are the main means for monitoring propofol in exhaled breath in real time. Takita et al used PTR-MS to monitor the propofol concentration during a single respiratory cycle of an intraoperative patient in real time and synchronously compared the temperature of the patient's respiratory gases. In addition, Elizarov et al and Grosshere et al also monitored the propofol trend in real-time within a single respiratory cycle of the intraoperative patient using EI-MS and IMR-MS, respectively. However, mass spectrometers are often expensive and bulky, which prevents large-scale clinical popularization to some extent.
Disclosure of Invention
IMS as a separation detection technology of atmospheric ions has the characteristics of low cost, high sensitivity, high response speed, easiness in carrying and the like, and a plurality of researches show that the IMS can realize the detection of propofol. Therefore, if the real-time online monitoring of propofol in exhaled breath by IMS can be realized, it is expected to become a noninvasive anesthesia monitoring or organ function monitoring means which can be popularized in a large area. For this reason we have invented a real-time online ion mobility spectrometry for real-time online monitoring of propofol in exhaled breath, in either positive or negative ion mode, respectively.
Drawings
FIG. 1: the ion mobility spectrometry comprises a reaction zone and a migration zone, wherein vacuum ultraviolet lamps 1, 2 and 3 are respectively arranged at two sides and the front ends 1, 2 and 3 of the reaction zone, 4 is a BN-grid ion gate, 5 is a conductive ring, 6 is a grid, 7 is a high-voltage power supply, 8 is an amplifier, 9 is drift gas of the migration tube, 10 is an expired gas sample, 11 is reagent molecule carrier gas, 12 is anisole reagent molecules, 13 is a flow meter for controlling sampling flow rate, and 14 is a sampling pump;
FIG. 2: an ion mobility spectrum of propofol in a positive ion mode;
FIG. 3: an ion mobility spectrum of propofol in a negative ion mode;
Detailed Description
A method for monitoring exhaled propofol in real time on line comprises the steps that an ion mobility spectrum comprises a left reaction area and a right migration area, a first vacuum ultraviolet lamp photoionization source is arranged at the left end of the reaction area far away from the migration area, and a second vacuum ultraviolet lamp photoionization source and a third vacuum ultraviolet lamp photoionization source are respectively arranged on the upper side and the lower side of the reaction area and are used for efficient ionization of a high-flux sample in the reaction area;
floating gas enters the ion migration tube from the tail part of the right end of the migration region far away from the reaction region of the ion migration tube, an ion gate is arranged between the migration region and the reaction region of the ion migration tube, exhaled sample gas enters the ion migration tube from the reaction region close to the ion gate in the middle of the ion migration tube, the sample gas is continuously pumped into the ion migration tube through a gas outlet arranged at the left end of the ion reaction region, and the gas outlet is connected with a sampling pump through a mass flow meter;
a reagent molecule feeding port carried by carrier gas is arranged on the side wall surface of the reaction area on the left side of the sample gas inlet.
Under the normal operation state of the migration tube, under the action of a sampling pump, sample gas is collected into a reaction area in the migration tube in real time, meanwhile, reagent molecules continuously enter the reaction area of the ion migration tube and are photoionized by three vacuum ultraviolet lamps at the two sides and the left end of the reaction area to generate reaction reagent ions, the sample and the reaction reagent ions react in the reaction area to generate product ions of the sample, the product ions periodically enter the migration area from an ion gate under the action of an electric field of the reaction area and are separated under the action of the electric field of the migration area, and then reach a Faraday disc to be detected, and finally, an ion migration spectrogram of propofol is obtained.
The migration tube can detect propofol in a positive ion mode or a negative ion mode in real time on-line.
In positive ion mode, a positive 5kV high voltage is applied to the migration tube, and anisole is used as a reagent molecule.
In the negative ion mode, a negative 5kV high voltage is applied to the transfer tube, and acetone is used as a reagent molecule.
The floating gas enters the ion migration tube from the tail part of the right end of the migration zone far away from the reaction zone of the ion migration tube, an ion gate is arranged between the migration zone and the reaction zone of the ion migration tube, the sample gas enters the ion migration tube from the reaction zone close to the ion gate in the middle of the ion migration tube, the sample gas is continuously pumped into the ion migration tube through a gas outlet arranged at the left end of the ion reaction zone, and the gas outlet is connected with a sampling pump through a mass flow meter.
The flow meter before the sampling pump is set at 200-600ml/min, the flow rate of the floating gas is set at 100-500ml/min, the flow rate of the reagent molecular carrier gas is 50ml/min, and the flow rate of the sample gas pumped into the migration pipe is 50-500 ml/min.
The air outlet is positioned on the side wall surface of the reaction zone at the left side of the optical window of the second or third vacuum ultraviolet lamp and at the right side of the optical window of the first vacuum ultraviolet lamp.
Example 1
Detecting in positive ion mode, applying positive 5kV high voltage on the migration tube, anisole reagent molecule, drift gas flow rate of 200ml/min, reagent molecule carrier gas flow rate of 50ml/min, flow rate set by the flowmeter of 300ml/min, and sampling exhalation gas flow rate of 50 ml/min. Under the normal operation state of the migration tube, under the action of a sampling pump, sample gas is collected into a reaction region in the migration tube in real time, anisole reagent molecules continuously enter the reaction region of the ion migration tube, and are photoionized by three vacuum ultraviolet lamps at the upper side, the lower side and the left end of the reaction region to generate reaction reagent ions, the sample and the reaction reagent ions react in the reaction region to generate product ions of the sample, the product ions periodically enter the migration region from an ion gate under the action of an electric field of the reaction region, are separated under the action of the electric field of the migration region, and sequentially reach a Faraday disc for detection, and finally, a spectrogram of the real-time exhaled propofol is obtained, as shown in figure 2. Wherein, the reagent ion is the molecular ion peak Dopant of anisole+The product ion of Propofol is Propofol+The detection sensitivity of propofol in the mode can reach 20 pptv.
Example 2
Detecting in negative ion mode, applying negative 5kV high voltage on the migration tube, acetone reagent molecule flow rate is 200ml/min, reagent molecule carrier gas flow rate is 50ml/min, flow rate set by the flowmeter is 300ml/min, and flow rate of exhaled air for sampling is 50 ml/min. Under the normal operation state of the transfer pipeUnder the action of a sampling pump, sample gas is collected into a reaction area in a migration tube in real time, anisole reagent molecules continuously enter the reaction area of the ion migration tube, are photoionized by three vacuum ultraviolet lamps at the upper side, the lower side and the left end of the reaction area to generate reaction reagent ions, the sample and the reaction reagent ions react in the reaction area to generate product ions of the sample, the product ions periodically enter the migration area from an ion gate under the action of an electric field of the reaction area, are separated under the action of the electric field of the migration area, and sequentially reach a Faraday disc for detection, and finally a spectrogram of the real-time exhaled propofol is obtained, as shown in figure 3. Wherein the reagent ion is O2 -The product ion of Propofol is Propofol.O2 -The detection sensitivity of propofol in the mode can reach 60 pptv.
Claims (8)
1. A method for real-time online monitoring of exhaled propofol is characterized by comprising the following steps:
the ion mobility spectrometry comprises a left reaction zone and a right migration zone, a first vacuum ultraviolet lamp photoionization source is arranged at the left end of the reaction zone far away from the migration zone, and a second vacuum ultraviolet lamp photoionization source and a third vacuum ultraviolet lamp photoionization source are respectively arranged at the upper side and the lower side of the reaction zone and are used for high-efficiency ionization of a high-flux sample in the reaction zone;
floating gas enters the ion migration tube from the tail part of the right end of the migration region far away from the reaction region of the ion migration tube, an ion gate is arranged between the migration region and the reaction region of the ion migration tube, exhaled sample gas enters the ion migration tube from the reaction region close to the ion gate in the middle of the ion migration tube, the sample gas is continuously pumped into the ion migration tube through a gas outlet arranged at the left end of the ion reaction region, and the gas outlet is connected with a sampling pump through a mass flow meter;
a reagent molecule feeding port carried by carrier gas is arranged on the side wall surface of the reaction area on the left side of the sample gas inlet.
2. The method of claim 1, wherein:
under the normal operation state of the migration tube, under the action of a sampling pump, sample gas is collected into a reaction area in the migration tube in real time, meanwhile, reagent molecules continuously enter the reaction area of the ion migration tube and are photoionized by three vacuum ultraviolet lamps at the two sides and the left end of the reaction area to generate reaction reagent ions, the sample and the reaction reagent ions react in the reaction area to generate product ions of the sample, the product ions periodically enter the migration area from an ion gate under the action of an electric field of the reaction area and are separated under the action of the electric field of the migration area, and then reach a Faraday disc to be detected, and finally, an ion migration spectrogram of propofol is obtained.
3. The method of claim 1, wherein: the migration tube can detect propofol in a positive ion mode or a negative ion mode in real time on-line.
4. The method of claim 3, wherein: in positive ion mode, a positive 5kV high voltage is applied to the migration tube, and anisole is used as a reagent molecule.
5. The method of claim 3, wherein: in the negative ion mode, a negative 5kV high voltage is applied to the transfer tube, and acetone is used as a reagent molecule.
6. The method of claim 1, wherein: the floating gas enters the ion migration tube from the tail part of the right end of the migration zone far away from the reaction zone of the ion migration tube, an ion gate is arranged between the migration zone and the reaction zone of the ion migration tube, the sample gas enters the ion migration tube from the reaction zone close to the ion gate in the middle of the ion migration tube, the sample gas is continuously pumped into the ion migration tube through a gas outlet arranged at the left end of the ion reaction zone, and the gas outlet is connected with a sampling pump through a mass flow meter.
7. The method of claim 1, wherein: the flow meter before the sampling pump is set at 200-600ml/min, the flow rate of the floating gas is set at 100-500ml/min, the flow rate of the reagent molecular carrier gas is 50ml/min, and the flow rate of the sample gas pumped into the migration pipe is 50-500 ml/min.
8. The method of claim 1, wherein: the air outlet is positioned on the side wall surface of the reaction zone at the left side of the optical window of the second or third vacuum ultraviolet lamp and at the right side of the optical window of the first vacuum ultraviolet lamp.
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| CN103871828A (en) * | 2012-12-17 | 2014-06-18 | 中国科学院大连化学物理研究所 | Array type photoelectric transmission ionizing source and application thereof |
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| CN106872553A (en) * | 2015-12-14 | 2017-06-20 | 中国科学院大连化学物理研究所 | A kind of Propofol detection method for eliminating sevoflurane interference |
| CN108088891A (en) * | 2016-11-21 | 2018-05-29 | 中国科学院大连化学物理研究所 | A kind of ion mobility spectrometry and operating method for being disposed vertically VUV radio-frequency lamps |
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2018
- 2018-11-25 CN CN201811411820.4A patent/CN111220683A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101713762A (en) * | 2008-10-07 | 2010-05-26 | 中国科学院大连化学物理研究所 | Method for identifying and detecting halogenated hydrocarbons |
| CN102221576A (en) * | 2010-04-15 | 2011-10-19 | 岛津分析技术研发（上海）有限公司 | Method and device for generating and analyzing ions |
| CN102455319A (en) * | 2010-10-29 | 2012-05-16 | 中国科学院大连化学物理研究所 | Method for monitoring propofol narcotic in on-line manner |
| CN102324376A (en) * | 2011-09-28 | 2012-01-18 | 上海大学 | Compensation irradiating type vacuum ultraviolet lamp ion source device |
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