CN107957457B - Auxiliary device of gas chromatography and antenna potential combined instrument - Google Patents
Auxiliary device of gas chromatography and antenna potential combined instrument Download PDFInfo
- Publication number
- CN107957457B CN107957457B CN201711279338.5A CN201711279338A CN107957457B CN 107957457 B CN107957457 B CN 107957457B CN 201711279338 A CN201711279338 A CN 201711279338A CN 107957457 B CN107957457 B CN 107957457B
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- gas chromatograph
- antennal
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/64—Electrical detectors
Abstract
The invention relates to the technical field of antenna electrophysiology, and discloses an auxiliary device of a gas chromatograph and antenna potential combined instrument, which comprises a U-shaped three-way pipe, wherein a first channel is arranged at the bottom edge of the U-shaped of the three-way pipe, a second channel and a third channel are respectively arranged at two side edges of the U-shaped of the three-way pipe, the first channel is communicated with a branch capillary column of a splitter valve of a gas chromatograph, the second channel is communicated with a sample inlet of an L-shaped glass tube of the antenna potential instrument, and the third channel is a supplementary carrier gas inlet. The auxiliary device of the gas chromatograph-antenna potential combined instrument provided by the invention provides supplementary carrier gas for the sample which flows to the antenna and is shunted by the shunt valve of the gas chromatograph, thereby effectively improving the detection sensitivity of the gas chromatograph-antenna potential combined instrument.
Description
Technical Field
The invention relates to the technical field of antenna electrophysiology, in particular to an auxiliary device of a gas chromatograph and antenna potential combined instrument.
Background
Because the antenna potential technology has the characteristics of extremely high selectivity and sensitivity, the antenna potential technology is widely applied to the research of the interrelation of insects and plants. The invention and the use of capillary column chromatography at the end of the fifties of the twentieth century greatly improved the separation performance of Gas Chromatography (GC). However, gas chromatography can only qualitatively and quantitatively analyze compounds and cannot provide information on the biological activity of the test substance. Under this opportunity, a Gas Chromatography (GC) combined with an antenna potential (EAG) technique (GC-EAD) was developed. The basic principle is as follows: after being injected by a GC injector, the sample is separated by a capillary chromatographic column and then is shunted according to a certain proportion by a shunt valve. One part of the shunted sample passes through the capillary column to the flame ionization detector, the other part of shunted sample passes through the same capillary column and then is conveyed to the L-shaped glass tube through a capillary column conveying tube, and then is carried to the insect antenna between the glass electrodes by humid air to carry out antenna potential measurement of the sample, thereby detecting substances with the electrophysiological activity of the insect antenna in the sample. The technology fully combines the advantages of high separation efficiency of gas chromatography and high sensitivity and high selectivity of insect antennae to achieve the rapid separation and identification of the activity of volatile matters. This technique has since been further improved and used in many pheromone studies. At present, the GC-EAG technology is widely applied to the separation and identification of sex pheromone and the screening of plant-derived insect attractants and repellents.
In practice, we found that when a sample is detected by an antennal potentiostat alone, an antennal electrophysiological active substance can be detected in the sample, but when the sample is detected by a gas chromatography-antennal potentiostat combination, the antennal electrophysiological active substance is not detected in the sample. The reason for this is that on the one hand the concentration of the antennal electrophysiological active substance in the shunted sample is inherently low, and on the other hand the velocity of the gas stream after shunting is so slow that less antennal electrophysiological active substance reaches the antenna per unit time, at a concentration below the threshold value of the electrophysiological response of the insect antenna. Therefore, the electrophysiological active substances of the antennae cannot be detected, and the sensitivity of the instrument is insufficient from the instrument viewpoint. Resulting in partial or complete information loss during sample analysis, and limiting the use of the gas chromatography-antennal potential combination instrument.
Disclosure of Invention
Technical problem to be solved
The invention provides an auxiliary device of a gas chromatography-antenna potential combined instrument, which aims to solve the problem of insufficient sensitivity when the gas chromatography-antenna potential combined instrument detects a sample.
(II) technical scheme
In order to solve the technical problem, the invention provides an auxiliary device of a gas chromatograph and antenna potentiometer combined instrument, which comprises a generally U-shaped three-way pipe, wherein a first channel is arranged at the bottom edge of the U-shaped of the three-way pipe, two side edges of the U-shaped of the three-way pipe are a second channel and a third channel, the first channel is communicated with a branch capillary column of a gas chromatograph splitter valve, the second channel is communicated with a sample inlet of an L-shaped glass tube of the antenna potentiometer, the second channel comprises a wide-caliber section, a gradual change section and a small-caliber section which are of an integrated structure, the aperture of the gradual change section is gradually reduced, the caliber of the small-caliber section is matched with the aperture of a sample inlet of the L-shaped glass tube, the tail end of the small-caliber section is inserted into the sample inlet of the L-shaped glass tube, and the small-caliber section is 2cm long; the third passageway is a supplemental carrier gas inlet.
Further, the first channel is provided with a sealing plug, and the capillary column penetrates through the sealing plug to be communicated with the three-way pipe.
Furthermore, the end surface of the tail end of the capillary column after penetrating through the sealing plug and the inner end surface of the sealing plug are in the same plane, and the inner end surface of the sealing plug is flush with the inner surface of the pipe wall of the bottom edge of the U-shaped tee pipe.
Further, an air purifier is arranged on the antenna potentiometer, an air shunt valve is arranged on an air outlet pipeline of the air purifier, the air shunt valve is divided into two branches, one branch is connected with a first air pipeline, the first air pipeline is communicated with the head end of the L-shaped glass tube, and a humidifier is arranged on the first air pipeline; the other branch is communicated with a second air pipeline which is communicated with a third channel.
Further, the first air pipeline and the second air pipeline are silicone tubes.
Further, the three-way pipe is a glass pipe.
(III) advantageous effects
The technical scheme of the invention has the following advantages: the auxiliary device of the gas chromatograph-electroantennal potentiometer provided by the invention is characterized in that the three-way pipe is arranged between the capillary column of the gas chromatograph and the L-shaped glass pipe of the electroantennal potentiometer, so that a supplementary carrier gas can be provided for a sample which flows to the electroantennal and is divided by the splitter of the gas chromatograph, the speed of the sample flowing into the L-shaped glass pipe is increased, the concentration of the sample reaching the electroantennal in unit time is also increased, and the detection sensitivity of the gas chromatograph-electroantennal potentiometer is effectively improved.
In addition to the technical problems addressed by the present invention, the technical features constituting the technical solutions and the advantages brought by the technical features of the technical solutions described above, other technical features of the present invention and the advantages brought by the technical features of the technical solutions will be further explained with reference to the accompanying drawings.
Drawings
FIG. 1 is an exploded view of a gas chromatograph and antennal potentiometer combination auxiliary device and a gas chromatograph and antennal potentiometer combination device according to an embodiment of the invention;
FIG. 2 is a schematic diagram of the combination of the gas chromatograph and the auxiliary device of the combined antenna potentiometer and capillary column according to the embodiment of the invention;
FIG. 3 is a schematic view of the combination of the auxiliary device of the gas chromatograph/antennal potentiometer combined instrument and the L-shaped glass tube according to the embodiment of the invention.
In the figure: 1: gas chromatograph, 11: sample injector, 12: column, 13: sample diverter valve, 14: flame ion detector, 15: a capillary column; 21: l-shaped glass tube, 22: air purifier, 23: a humidifier; 3: three-way pipe, 31: first passage, 32: second passage, 33: third passage, 34: a sealing plug; 5, an air shunt valve.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
In addition, in the description of the present invention, unless otherwise specified, "plurality", "plural groups" means two or more, and "several", "several groups" means one or more.
As shown in fig. 1, an auxiliary device for a gas chromatograph and an antennal potentiometer according to an embodiment of the present invention is disposed between a gas chromatograph 1 and the antennal potentiometer for transporting a sample from the gas chromatograph.
As shown in fig. 1, the gas chromatograph 1 is provided with an injector 11, a capillary column 12, a sample split valve 13, and a flame ion detector 14. The electroantennogram instrument is provided with an air purifier 22, a humidifier 23, an L-shaped glass tube 21 and an antenna (not shown in the figure, the antenna is arranged at the tail end of the L-shaped glass tube); wherein, the end face of the head end of the L-shaped glass tube 21 is provided with an air inlet, the side face of the L-shaped glass tube 21 close to the head end is provided with a sample inlet, and the tail end of the L-shaped glass tube 21 is an outlet of a mixture of a sample and air; the air purifier 22 is communicated with an air inlet of the L-shaped glass tube 21 through a first air outlet tube, and the humidifier 23 is arranged on the first air pipeline for humidifying air.
The auxiliary device of the gas chromatograph and antenna potential combined instrument is arranged between the capillary column 15 of a sample outlet of the sample splitter valve 13 and the sample inlet of the L-shaped glass tube 21.
As shown in fig. 2 or 3, the auxiliary device for a gas chromatograph and antennal potential combined instrument in this embodiment includes a substantially U-shaped three-way pipe 3, a first channel 31 is communicated with a bottom edge of the U-shape of the three-way pipe 3, a second channel 32 and a third channel 33 are respectively disposed on two side edges of the U-shape of the three-way pipe 3, the first channel 31 is communicated with one branched capillary column 15 of the gas chromatograph sample splitter valve 13, the second channel 32 is communicated with a sample inlet of an L-shaped glass tube 21 of the antennal potential instrument, and the third channel 33 is a supplementary carrier gas inlet.
As shown in fig. 1, a sample to be detected is injected by an injector 11 of a gas chromatograph 1, then is separated by a capillary chromatographic column 12, and is split by a sample splitter valve 13 according to a certain proportion, a part of the split sample enters a flame ion detector 14 through the capillary column, and the other part of the split sample enters an L-shaped glass tube 21 through the capillary column 15 under the driving action of air introduced by an auxiliary device of the gas chromatograph and antenna potential combined instrument in the embodiment of the invention, and is carried to an insect antenna between glass electrodes by humid air, so as to measure the antenna potential of the sample.
The auxiliary device of the gas chromatograph-antennal potential combined instrument provided by the invention is arranged on a capillary column 15 of a sample shunt valve 13 flowing to an antennal potential instrument, and provides supplementary carrier gas for shunting the gas chromatograph flowing to an antennal, so that the movement speed of a sample is improved, and substances with the electrophysiological activity of the antennal reaching the antennal in unit time are increased, so that the threshold value of the electrophysiological reaction of the insect antennal is reached, the detection requirement of the antennal potential instrument is met, and the detection sensitivity of the gas chromatograph-antennal potential instrument is correspondingly and effectively improved.
The first channel 31 and the capillary column 15 are sealed by a sealing plug 34, specifically, as shown in fig. 2, the sealing plug 34 is disposed on the first channel 31, and the capillary column 15 passes through the sealing plug 34 to communicate with the three-way pipe 3. During specific setting, the end surface of the capillary column 15 after passing through the sealing plug 34 and the inner end surface of the sealing plug 34 are in the same plane, and the inner end surface of the sealing plug 34 is flush with the inner surface of the pipe wall of the U-shaped bottom end surface of the three-way pipe 3; i.e. the sealing plug 34 can fill the entire first passage and not extend under the passage space of the U-shaped bottom surface of the tee.
As shown in fig. 2 or 3, the second channel 32 is a wide-diameter section, a gradual change section and a small-diameter section which are connected in sequence, wherein the diameter of the gradual change section is gradually reduced, the diameter of the small-diameter section is matched with the diameter of the sample inlet of the L-shaped glass tube, and the end of the small-diameter section is inserted into the sample inlet of the L-shaped glass tube. It will be appreciated that the second channel 32 is configured to further accelerate the flow rate of the sample and concentrate the flow concentration of the sample per unit time, typically in a particular arrangement where the small pore diameter section is about 2cm in length.
The supplementary carrier gas of the third channel 33 can come from an air purifier 22 carried by the electroantennogram instrument, specifically, an air splitter valve 5 is arranged on an air outlet pipeline of the air purifier 22, the air splitter valve 5 is divided into two branches, and one branch is communicated with the first air pipeline to provide carrier gas for a sample in the L-shaped glass tube 21; the other branch is connected with a second air pipeline which is communicated with the third channel 33. It will be appreciated that the air splitter valve may adjust the respective gas flow rates of the two air lines; the additional carrier gas directly uses the second air line connected with the air purifier 22, and no additional air purifying equipment is needed, thereby saving the investment of the equipment and correspondingly simplifying the composition of the equipment.
Preferably, the three-way pipe 3 of the auxiliary device of the gas chromatograph and antenna potential combined instrument is a glass pipe, and the first air pipeline and the second air pipeline are silicone tubes.
In summary, the auxiliary device of the gas chromatograph and antenna potential combined instrument has the following advantages: an air purifier of the electroantennogram instrument is used for providing supplementary carrier gas for the shunting of the gas chromatography capillary column flowing to the electroantennogram, so that the detection sensitivity of the gas chromatography-electroantennogram is effectively improved; in addition, as the capillary column transmission pipe is not used any more, but the form of the second channel of the three-way pipe is adopted, the burning of high temperature to the in vitro antenna can be avoided, the actual service life of the in vitro insect antenna is prolonged, and the detection effect is improved; the system is simple to operate; the used materials are easy to obtain and the cost is low.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (6)
1. The utility model provides an auxiliary device of gas chromatography and antennal potential allies oneself with appearance which characterized in that: the sampling device comprises a generally U-shaped three-way pipe, wherein a first channel is arranged at the bottom edge of the U shape of the three-way pipe, a second channel and a third channel are arranged at two side edges of the U shape of the three-way pipe, the first channel is communicated with a branch capillary column of a gas chromatography flow divider, the second channel is communicated with a sample inlet of an L-shaped glass pipe of an electroantennogram, the second channel comprises a wide-caliber section, a gradual change section and a small-caliber section which are of an integrated structure, the aperture of the gradual change section is gradually reduced, the aperture of the small-caliber section is matched with the aperture of a sample inlet of the L-shaped glass pipe, the tail end of the small-caliber section is inserted into the sample inlet of the L-shaped glass pipe, and the small-caliber section is 2cm in length; the third passageway is a supplemental carrier gas inlet.
2. The apparatus for assisting a gas chromatograph and antennal potentiometry combined with one or more of claims 1, wherein: the first channel is provided with a sealing plug, and the capillary column penetrates through the sealing plug to be communicated with the three-way pipe.
3. The apparatus for assisting a gas chromatograph and antennal potentiometry combined with one or more of claims 2, wherein: the end face of the tail end of the capillary column after penetrating through the sealing plug and the inner end face of the sealing plug are in the same plane, and the inner end face of the sealing plug is flush with the inner surface of the pipe wall of the U-shaped bottom edge of the three-way pipe.
4. The apparatus for assisting a gas chromatograph and antennal potentiometry combined with one or more of claims 1, wherein: an air purifier is arranged on the antenna potentiometer, an air shunt valve is arranged on an air outlet pipeline of the air purifier, the air shunt valve is divided into two branches, one branch is connected with a first air pipeline, the first air pipeline is communicated with the head end of the L-shaped glass tube, and a humidifier is arranged on the first air pipeline; the other branch is communicated with a second air pipeline which is communicated with a third channel.
5. The apparatus for assisting a gas chromatograph and antennal potentiometry combined with one or more of claims 4, wherein: the first air pipeline and the second air pipeline are silicone tubes.
6. The combination gas chromatograph and antennal potentiometric aid of any one of claims 1 to 4, wherein: the three-way pipe is a glass pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711279338.5A CN107957457B (en) | 2017-12-06 | 2017-12-06 | Auxiliary device of gas chromatography and antenna potential combined instrument |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711279338.5A CN107957457B (en) | 2017-12-06 | 2017-12-06 | Auxiliary device of gas chromatography and antenna potential combined instrument |
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| Publication Number | Publication Date |
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| CN107957457A CN107957457A (en) | 2018-04-24 |
| CN107957457B true CN107957457B (en) | 2020-09-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201711279338.5A Expired - Fee Related CN107957457B (en) | 2017-12-06 | 2017-12-06 | Auxiliary device of gas chromatography and antenna potential combined instrument |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108562668A (en) * | 2018-05-02 | 2018-09-21 | 中国农业科学院烟草研究所 | A kind of method of GC-MS and tentaculum electric potential instrument combination analysis insect infochemical |
| CN115212701A (en) * | 2022-07-22 | 2022-10-21 | 重庆钢铁股份有限公司 | Coal gas dehydration purifier |
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| CN102818871B (en) * | 2012-05-29 | 2014-10-15 | 上海烟草集团有限责任公司 | Interface device and method for on-line coupling of liquid chromatography-gas chromatography/mass spectrum (LC-GC/MS) |
| CN105158380B (en) * | 2015-05-13 | 2020-03-31 | 上海烟草集团有限责任公司 | Interface device for high performance liquid chromatography and gas chromatography/mass spectrometry |
| CN106093266B (en) * | 2016-08-16 | 2018-02-06 | 中国科学院西双版纳热带植物园 | The GC EAD systems that chromatographic column constant temperature transmission line is formed |
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Granted publication date: 20200925 Termination date: 20211206 |