CN109331893A - Micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus - Google Patents
Micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus Download PDFInfo
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- CN109331893A CN109331893A CN201811397904.7A CN201811397904A CN109331893A CN 109331893 A CN109331893 A CN 109331893A CN 201811397904 A CN201811397904 A CN 201811397904A CN 109331893 A CN109331893 A CN 109331893A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5023—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures with a sample being transported to, and subsequently stored in an absorbent for analysis
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
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Abstract
The present invention relates to a kind of micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus.The device includes: micro-fluidic control device, microfluidic separation device, array aerosolizing part and the mass spectrometry components for controlling loading and mobile phase or buffer solution flowing.Microfluidic separation device is by the fixed phase composition of micro-fluidic support plate, micro-fluidic cover board and paper, it is coated on support plate for providing the metal electrode of separation electric field, there are the channels transported for loading and mobile phase for front end on cover board, there are the disengagement chambers for separation and paper fillers stationary phase at middle part, disengagement chamber end is row's fraction outlets, paper stationary phase is filled in disengagement chamber, for separating object；Fraction outlets connect array spraying device, after array spraying device is spraying, are analyzed by mass spectrometry.Present invention incorporates free stream cataphoresis, chromatography and mass spectrographic advantages, highly effective can improve the separation detection efficiency of traditional free stream cataphoresis, not only horizontally be separated using electric field action, and further function as centrifugation according to chromatographic mechanism in the longitudinal direction.
Description
Technical field
The present invention relates to combined gas chromatography mass spectrometry fields, and in particular to a kind of micro-fluidic free flow paper chromatography array electrospray mass spectrometry
Combined apparatus.
Background technique
Between Past 30 Years, the development of two dimensional separation analytical technology is very rapid, is widely used in complex biological, chemistry, doctor
It learns and the separation of the samples such as environment is analyzed.This separation analysis means sharpest edges are the peak capacity for having high, so far for
Only there are many two dimensional separation means, including Capillary Electrophoresis-high performance liquid chromatography combination, Capillary Electrophoresis-Capillary Electrophoresis
Combination etc., all achieves preferable effect.Microfluidic chip technology is based on the advantages such as Highgrade integration, high throughput, low consumption,
So become one of the important means of life science.It is using chip as basic platform, and by analytical chemistry means, process is micro electronmechanical
The technologies such as processing, establish microchannel network, and integrating includes the processes such as sample pretreatment, separation, purifying, detection, and can be more
Secondary reuse.Microfluidic chip technology has convenient for control, easy to operate, needs sample few, and it is excellent that separation analysis speed is fast etc.
Gesture, while it being based on its Highgrade integration feature, separation analysis can be carried out to multiple samples simultaneously.Based on features above, miniflow
Control chip is also referred to as chip lab, it is seen that it is huge in the potential value of the fields such as biology, chemistry, medicine, environment application.
Free flow electrochromatography combines chromatography and free stream cataphoresis mechanism, while fixing according to electromobility and with mobile phase
Interact between phase it is different separated, peak capacity and separative efficiency can be effectively improved.In recent years, this technology is extensive
In preparation for the high kurtosis component in complex biological sample, however since it requires applied sample amount larger, and it is not easy to pair
Small lot sample preparation and separation analysis.The porous characteristic of paper itself can be used for volume exclusion separation, while long-chain fiber itself
The characteristic of element can interact with sugared a kind of property similar substance, to be a kind of pair of highly effective chromatography of specific objective object
Stationary phase.
Protein glycosylation is to be currently known one of most important posttranslational modification mode, is played in many cell processes
Highly important effect, and it is related to a large amount of diseases, have a large amount of glycoprotein at present and is used to face as biomarker
Bed detection.However current glycoprotein separate mode is based on the means such as liquid chromatogram, gel chromatography, Solid Phase Extraction, these sides
It is some there are low separation efficiency in method, the problems such as some presence are time-consuming more.
Therefore, in view of problem above, the present invention designs by research and innovation and has manufactured a kind of micro-fluidic free flow paper color
Spectrum array electrospray mass spectrometry combined apparatus realizes the online two dimensional separation analysis of Highgrade integration, can effectively improve point of sample
From efficiency, directly it is connected with array spraying device, target sample can be ionized directly to and be entered mass spectral analysis, operation letter
Just, cheap, integrated level is high, makes it with more the utility value in industry.
Summary of the invention
To solve at least one above-mentioned existing defect (deficiency), it is an object of that present invention to provide a kind of micro-fluidic free flow paper
Chromatography array electrospray mass spectrometry combined apparatus carries out including sample pretreatment, online two dimensional separation, array for Highgrade integration
It is spraying, Mass Spectrometer Method, to reach the peak capacity for improving complex sample separation analysis, separative efficiency and detection efficiency.
A kind of micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus proposed by the present invention, including micro-fluidic control
Device and microfluidic separation device, array aerosolizing part and mass spectrometry components, in which: micro-fluidic control device includes micro-fluidic load
Plate, micro-fluidic cover board and paper stationary phase are evenly distributed with several nozzle needles on array aerosolizing part.The front end of micro-fluidic cover board is equipped with
It is transported for mobile phase and the microfluidic channel and sample channel of loading, the separation for paper fillers stationary phase is equipped in middle part
Chamber, end are equipped with row's fraction outlets；Micro-fluidic cover board is placed on micro-fluidic support plate, and disengagement chamber is made to be in the environment of sealing；It is micro-
Flow control one end of the channel connects first pipe one end, and the first pipe other end connects micro-fluidic control device, buffer or mobile phase
Entered in disengagement chamber by first pipe through microfluidic channel, sample channel one end connects second pipe one end, and second pipe is another
End connects micro-fluidic control device, and sample is entered in disengagement chamber by second pipe through sample channel, and fraction outlets are defeated by fraction
Channel connects capillary one end out, and the capillary other end connects array aerosolizing part nozzle needle, and metal electricity is coated on micro-fluidic support plate
Pole, for providing separation electric field, paper stationary phase is filled in the chromatographic isolation that sample is used in disengagement chamber；Array aerosolizing part nozzle needle
Outlet connection mass spectrometry components, the mass spectrometry components detect the sample after array electro-spray ionization.
In the present invention, one or more of peristaltic pump, mechanical pump or syringe pump is can be selected in micro fluidic device, is preferably infused
Penetrate pump.
In the present invention, the sample feeding flow velocity is the mL/min of 3 nL/min ~ 10, and buffer or flow rate of mobile phase are 3
nL/min ~ 10 mL/min。
In the present invention, micro-fluidic support plate and micro-fluidic cover board material can be any in plastics, colloid or glass.
In the present invention, it is coated with metal electrode on micro-fluidic support plate, which can be in the conductive metals such as gold, silver, copper or aluminium
It is any.
Applying voltage range in the present invention, on metal electrode is the V of 10 V ~ 5000.
In the present invention, micro-fluidic support plate size outline about the size of the cover plate, in order to be powered on metal electrode.
In the present invention, the injection port quantity of the sample channel is 1 ~ 50, and each injection port bore is 1 nm ~ 5
mm。
In the present invention, the buffer or mobile phase entrance number of the microfluidic channel are 1 ~ 50, buffer or stream
Dynamic phase inlet calibre is the mm of 1nm ~ 5.
In the present invention, disengagement chamber grows the cm of 1 mm ~ 50, the width cm of 1 mm ~ 50, with a thickness of 10 in micro-fluidic cover board
nm ~ 10 mm。
In the present invention, micro-fluidic cover panel end fraction output channel number is 1 ~ 200, and output channel exit caliber is
1 μm ~ 1 mm。
In the present invention, micro-fluidic cover panel end fraction outlets number is 1 ~ 200.
In the present invention, paper stationary phase can select qualitative filter paper, quantitative filter paper, printing paper, function according to separation object difference
Paper can be changed.
In the present invention, the size of paper stationary phase and the interior disengagement chamber of micro-fluidic cover board match.
In the present invention, array spraying device is one group of link nozzle needle or itself end is the capillary of nozzle needle.
In the present invention, the capillary inner diameter of array spraying device is 1 μm ~ 1 mm.
In the present invention, array spraying device nozzle needle bore is 1 μm ~ 1 mm.
In the present invention, mass spectrometry components select any in flight time mass spectrum, quadrupole rod mass spectrum or ion trap mass spectrometry.
Under the micro-fluidic control device driving, mobile phase is full of disengagement chamber first, is applied after stablizing through metal electrode
Making alive, and by micro fluidic device loading, difference of the sample on the one hand under electric field action according to electromobility is separated,
On the other hand under paper stationary phase and flowing phase separation, chromatographic isolation, sample after separation are carried out according to volume exclusion and interaction
Product are directly entered in array spraying system, the Mass Spectrometer Method after spraying, reach the separation analysis of object high-speed and high-efficiency high sensitivity.
Compared with prior art, the device have the advantages that are as follows:
1, free stream cataphoresis, size exclusion chromatograph, paper chromatography technology height is integrated in micro-fluidic chip；
2, two dimensional separation effectively improves separative efficiency；
3, it is directly connected to that array is spraying and mass spectrum, improves detection efficiency and sensitivity；
4, design is simple, integration technology, saves space, and can freely adjust according to target sample complexity and sample size.
Detailed description of the invention
Fig. 1 is micro-fluidic free paper chromatography array electrospray mass spectrometry combined apparatus schematic diagram.
Fig. 2 is the micro-fluidic support plate partial schematic diagram in micro-fluidic free flow Paper chromatography device.
Fig. 3 is the micro-fluidic deck portion schematic diagram in micro-fluidic free flow Paper chromatography device.
Fig. 4 is the more array carrying board schematic diagrames of micro-fluidic free flow Paper chromatography device.
Fig. 5 is the more array installation diagrams of micro-fluidic free flow Paper chromatography device.
Figure label: 1 is first pipe, and 2 be disengagement chamber, and 3 be second pipe, and 4 be micro-fluidic support plate, and 5 be metal electrode,
6 be micro-fluidic cover board, and 7 be microfluidic channel, and 8 be sample channel, and 9 be fraction output channel, and 10 be paper stationary phase, and 11 be array
Aerosolizing part, 12 be capillary, and 13 be array aerosolizing part nozzle needle, and 14 be micro-fluidic control device.
Specific embodiment
Explanation is described in detail embodiment of the invention and instructs how those skilled in the art realize this below
The reproduction of invention.In order to instruct technical solution of the present invention, some conventional aspects for having simplified or having omitted.Those skilled in the art
It should be understood that from these embodiments deformation or will within the scope of the invention.Those skilled in the art should understand that following
Feature can be combined in various ways to form multiple deformations of the invention.The invention is not limited to the optional realities of subordinate as a result,
Mode is applied, and only claim and their equivalent limits.
Embodiment 1:
Such as Fig. 1, shown in 2,3, micro-fluidic free paper chromatography array electrospray mass spectrometry combined apparatus includes microfluidic control device 14, miniflow
Control free flow Paper chromatography device, that is, micro-fluidic support plate 4, micro-fluidic cover board 6, paper stationary phase 10 and array aerosolizing part
11, mass spectrometry components.In the present embodiment, paper stationary phase 10 is placed in the separation being made of micro-fluidic support plate 4 and micro-fluidic cover board 6
In chamber 2, guarantee device sealing.Buffer or mobile phase are entered in disengagement chamber 2 by first pipe 1 through microfluidic channel 7, sample by
Second pipe 3 enters to be entered in disengagement chamber 2 through sample channel 8；In the present embodiment, it is controlled using micro-fluidic control device 14
The flowing of sample preparation product loading, buffer or mobile phase controls.Peristaltic pump, mechanical pump, injection can be selected in micro-fluidic control device 14
One or more of pump, preferably syringe pump；Sample introduction flow velocity is the mL/min of 3 nL/min ~ 10, flow rate of mobile phase 3
nL/min ~ 10 mL/min.It is powered on to metal electrode 5, voltage range is the V of 10V ~ 5000；Electrode can be gold, silver, copper, aluminium
Equal conductive metals；Metal electrode be powered generate horizontal direction on electric field, perpendicular to direction of an electric field movement sample by level
The electric field force in direction, and the movement that deflects, to realize separation of the sample in horizontal dimensions.On longitudinal direction, paper chromatography is utilized
Separation principle, it is different using reservation of the different material in paper stationary phase, the separation in vertical dimension occurs in the present apparatus.
The fraction separated is connected from fraction output channel 9 through capillary 12, into array aerosolizing part 11.The spray of array aerosolizing part
The quantity of needle 13 can be 1 ~ 500, can also can be annular arrangement, to improve with parallel arranged when there is more nozzle needles
Ionization Efficiency obtains better Mass Spectrometer Method result.
In the present embodiment, it is preferable that microfluidic channel 7 is chosen as 1 ~ 50.
In the present embodiment, it is preferable that loading channel 8 is chosen as 1 ~ 50.
In the present embodiment, it is preferable that micro-fluidic cover panel end fraction output channel number is 1 ~ 200.
In the present embodiment, it is preferable that flight time mass spectrum, quadrupole rod mass spectrum or ion trap matter can be selected in mass spectrometry components
It is a kind of in spectrum.
Such as Fig. 4, shown in 5, the present apparatus can also be done micro-fluidic support plate greatly, realize that micro-fluidic chip array is integrated, support plate
13 are process by the duplication of single support plate 4 completely, in use, covering on each individual support plate region on support plate 13 micro-fluidic
Cover board, concrete operation step are consistent with one chip device.Micro-fluidic chip array is integrated to be easy to implement Multi-example, multicomponent
Analysis.
So the present invention effectively overcomes various shortcoming in the prior art and has high industrial utilization value.
The above-described embodiments merely illustrate the principles and effects of the present invention, and is not intended to limit the present invention.It is any ripe
The personage for knowing this technology all without departing from the spirit and scope of the present invention, carries out modifications and changes to above-described embodiment.Cause
This, institute is complete without departing from the spirit and technical ideas disclosed in the present invention by those of ordinary skill in the art such as
At all equivalent modifications or change, should be covered by the claims of the present invention.
Claims (11)
1. a kind of micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus, it is characterised in that: filled including micro-fluidic control
It sets, microfluidic separation device, array aerosolizing part and mass spectrometry components, in which: microfluidic separation device includes micro-fluidic support plate, micro-
Flow control cover board and paper stationary phase are evenly distributed with several nozzle needles on array aerosolizing part, and the front end of micro-fluidic cover board, which is equipped with, to be used for
Mobile phase transports and the microfluidic channel and sample channel of loading, and the disengagement chamber for paper fillers stationary phase, end are equipped in middle part
End is equipped with row's fraction outlets；Micro-fluidic cover board is placed on micro-fluidic support plate, and disengagement chamber is made to be in the environment of sealing；It is micro-fluidic logical
Road one end connects first pipe one end, and the first pipe other end connects micro-fluidic control device, and buffer or mobile phase are by first
Pipeline enters in disengagement chamber through microfluidic channel, and sample channel one end connects second pipe one end, the connection of the second pipe other end
Micro-fluidic control device, sample are entered in disengagement chamber by second pipe through sample channel, and fraction outlets pass through fraction output channel
Capillary one end is connected, the capillary other end connects array aerosolizing part nozzle needle, is coated with metal electrode on micro-fluidic support plate, is used for
Separation electric field is provided, paper stationary phase is filled in the chromatographic isolation that sample is used in disengagement chamber；Array aerosolizing part needle outlets connect
Mass spectrometry components are connect, the mass spectrometry components detect the sample after array electro-spray ionization.
2. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
It states micro-fluidic control device and selects one or more of peristaltic pump, mechanical pump or syringe pump.
3. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
Stating sample feeding flow velocity is the mL/min of 3 nL/min ~ 10, and buffer or flow rate of mobile phase are the mL/ of 3 nL/min ~ 10
min。
4. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
It states micro-fluidic support plate and micro-fluidic cover board material is any in plastics, colloid or glass.
5. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
It states and is coated with metal electrode on micro-fluidic support plate, which is any in gold, silver, copper or aluminium, application electricity on the metal electrode
Pressure is the V of 10 V ~ 5000.
6. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
The injection port quantity for stating sample channel is 1 ~ 50, and each injection port bore is the mm of 1 nm ~ 5；The microfluidic channel
Buffer or mobile phase entrance number are 1 ~ 50, and buffer or mobile phase inlet calibre are the mm of 1 nm ~ 5.
7. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
It states disengagement chamber in micro-fluidic cover board and grows the cm of 1 mm ~ 50, the width cm of 1 mm ~ 50, with a thickness of the mm of 10 nm ~ 10.
8. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
Stating micro-fluidic cover panel end fraction output channel number is 1 ~ 200, and output channel exit caliber is 1 μm ~ 1 mm.
9. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that: institute
Stating paper stationary phase can be according to separation object difference selection qualitative filter paper, quantitative filter paper, printing paper, functionalization paper.
10. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that:
The array spraying device is one group of link nozzle needle or itself end is the capillary of nozzle needle, the capillary of the array spraying device
Bore is 1 μm ~ 1 mm；Each array spraying device nozzle needle bore is 1 μm ~ 1 mm.
11. micro-fluidic free flow paper chromatography array electrospray mass spectrometry combined apparatus according to claim 1, it is characterised in that:
It is any in the mass spectrometry components selection flight time mass spectrum, quadrupole rod mass spectrum or ion trap mass spectrometry.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112916065A (en) * | 2021-05-11 | 2021-06-08 | 湖南师范大学 | Microfluidic paper chip, preparation method thereof, microfluidic paper chip detection system and application |
| WO2021120451A1 (en) * | 2019-12-17 | 2021-06-24 | 北京大学 | Flow cytometric analysis technique for organic mass spectrometry |
| CN113327836A (en) * | 2021-05-28 | 2021-08-31 | 深圳先进技术研究院 | Sample loading device for mass spectrometer |
| CN115069320A (en) * | 2022-07-01 | 2022-09-20 | 宁波工程学院 | Integrated chip of monolithic column nano-flow electroosmosis pump, manufacturing method, analysis system and application |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520330A (en) * | 2001-06-27 | 2004-08-11 | 比奥特罗韦有限公司 | System and method for high throughput sample propn. and analysis using column chromatography |
| WO2004100175A1 (en) * | 2003-05-08 | 2004-11-18 | The University Court Of The University Of St Andrews | Fractionation of particles |
| CN101206207A (en) * | 2006-12-22 | 2008-06-25 | 中国科学院大连化学物理研究所 | Multicenter electric spraying ion source for micro liquid phase separation system and mass spectrum |
| CN101885754A (en) * | 2010-06-23 | 2010-11-17 | 东华大学 | Method for continuously enriching and separating phosphoeptide at high throughput |
| CN102253159A (en) * | 2011-04-15 | 2011-11-23 | 中国烟草总公司郑州烟草研究院 | Method for analyzing tobacco flavor components by adopting multi-dimensional hyphenated chromatographic technique |
| CN102637574A (en) * | 2012-04-12 | 2012-08-15 | 清华大学 | Paper-base electrospray ion source device for mass spectrum analysis on continuous droplets |
| CN103033556A (en) * | 2012-12-28 | 2013-04-10 | 复旦大学 | Device and mass spectrometry for direct electrospray ionization of sample on microfluidic chip |
| CN103454439A (en) * | 2013-08-27 | 2013-12-18 | 中国科学院苏州生物医学工程技术研究所 | Integrated type sample feeding system for micro-fluidic chip |
-
2018
- 2018-11-22 CN CN201811397904.7A patent/CN109331893B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1520330A (en) * | 2001-06-27 | 2004-08-11 | 比奥特罗韦有限公司 | System and method for high throughput sample propn. and analysis using column chromatography |
| WO2004100175A1 (en) * | 2003-05-08 | 2004-11-18 | The University Court Of The University Of St Andrews | Fractionation of particles |
| CN101206207A (en) * | 2006-12-22 | 2008-06-25 | 中国科学院大连化学物理研究所 | Multicenter electric spraying ion source for micro liquid phase separation system and mass spectrum |
| CN101885754A (en) * | 2010-06-23 | 2010-11-17 | 东华大学 | Method for continuously enriching and separating phosphoeptide at high throughput |
| CN102253159A (en) * | 2011-04-15 | 2011-11-23 | 中国烟草总公司郑州烟草研究院 | Method for analyzing tobacco flavor components by adopting multi-dimensional hyphenated chromatographic technique |
| CN102637574A (en) * | 2012-04-12 | 2012-08-15 | 清华大学 | Paper-base electrospray ion source device for mass spectrum analysis on continuous droplets |
| CN103033556A (en) * | 2012-12-28 | 2013-04-10 | 复旦大学 | Device and mass spectrometry for direct electrospray ionization of sample on microfluidic chip |
| CN103454439A (en) * | 2013-08-27 | 2013-12-18 | 中国科学院苏州生物医学工程技术研究所 | Integrated type sample feeding system for micro-fluidic chip |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021120451A1 (en) * | 2019-12-17 | 2021-06-24 | 北京大学 | Flow cytometric analysis technique for organic mass spectrometry |
| CN113075114A (en) * | 2019-12-17 | 2021-07-06 | 北京大学 | Organic mass spectrometry flow cytometry analysis technology |
| CN112916065A (en) * | 2021-05-11 | 2021-06-08 | 湖南师范大学 | Microfluidic paper chip, preparation method thereof, microfluidic paper chip detection system and application |
| CN113327836A (en) * | 2021-05-28 | 2021-08-31 | 深圳先进技术研究院 | Sample loading device for mass spectrometer |
| CN115069320A (en) * | 2022-07-01 | 2022-09-20 | 宁波工程学院 | Integrated chip of monolithic column nano-flow electroosmosis pump, manufacturing method, analysis system and application |
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| CN109331893B (en) | 2021-07-23 |
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