CN209822593U - High-throughput single-cell electrospray mass spectrometry device - Google Patents
High-throughput single-cell electrospray mass spectrometry device Download PDFInfo
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- CN209822593U CN209822593U CN201920340599.1U CN201920340599U CN209822593U CN 209822593 U CN209822593 U CN 209822593U CN 201920340599 U CN201920340599 U CN 201920340599U CN 209822593 U CN209822593 U CN 209822593U
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Abstract
The utility model relates to a high flux unicellular electrospray mass spectrum device, including the support frame, horizontal fastening connection has the first three-way pipe and the second three-way pipe of series connection in the support frame, forms sample passageway on the horizontal direction, be provided with first, second, the long straight capillary spray needle of third on the sample passageway, the left end of the straight capillary spray needle of first length stretches out outside sample passageway, and the right-hand member setting is in the sample passageway, be close to the right-hand member of first three-way pipe, with set up the clamping device who connects two three-way pipes between the left end of second three-way pipe, first three-way pipe passes through the pipeline and links to each other with the injection pump the long straight capillary spray needle right side of third is provided with the mass spectrograph. The utility model discloses high flux unicellular electrospray mass spectrum device can realize that the sequencing of cell gets into the mass spectrograph and carries out the analysis, with the help of the quick analysis ability of mass spectrum and the ability of tens of thousands of materials of single detectable, can realize quick unicellular analysis, does not need the mark of metal or dyestuff simultaneously.
Description
Technical Field
The utility model belongs to the mass spectrometry field, in particular to high flux unicellular electrospray mass spectrum device.
Background
Mass spectrometry has been widely used as a highly sensitive and rapid analyzer in the fields of medicine, biology, food, chemistry, etc. Single cell analysis is a direction of great concern in modern biology and medicine, cells have heterogeneity, and cells in the same part of the same life body have differences, which is also a cause of cancer. Mass spectrometry methods for single intracellular substances and metabolic processes thereof have been reported, but the analysis speed is slow, and it is difficult to realize analysis and statistics of a large number of cells, which makes cell research and medical analysis difficult, and it is also difficult to perform personalized to regular research. The latest ICP-MS method for analyzing single cells can reach 1000 cells per minute, but is limited by instruments, only dozens of substances can be detected at a time, and the condition of the substances in the cells is indirectly analyzed by detecting the content of metal elements of the substances marked by metals.
In the life activity, the types of substances participating in the life activity are thousands of thousands, and the change is also an instant, so that the rapid and simultaneous analysis of more than one hundred substances in the content of a single cell can be realized by the capacity of simultaneous mass detection of multiple substances of the organic mass spectrum and the extremely high sensitivity of the capacity and the cooperation with a microfluidic cell processing method, and an effective tool is provided for the single cell life analysis.
CN107833821A discloses an open type double-tee capillary spray needle spraying device, which is formed by connecting two tee components in series, and a penetrating spray capillary is further arranged in the device, wherein two tee branches can respectively introduce gas and liquid, and the introduced gas is used for blowing and dispersing the liquid introduced by atomization. The utility model discloses a shortcoming is that it is fit for a large amount of liquid sample analysis, perhaps to the quick analysis of solid surface large tracts of land sample, only about 10 mu m to the diameter, and the volume can't carry out the analysis for pL's single cell.
CN104392886A discloses a capillary needle, which comprises a tube body, wherein two ends of the tube body are respectively an injection port and an outlet for performing electrospray, a part of the tube body is bent to form a sampling end, and the sampling end is provided with a sampling port communicated with the inner cavity of the tube body. Also discloses an electrospray mass spectrometry device adopting the capillary spray needle and a method for analyzing and detecting the electrospray mass spectrometry device. The utility model discloses a shortcoming can only be to liquid quick sampling analysis, hardly realize accurate quick sample and analysis to the single cell of extremely small volume in the liquid, more difficult single cell analysis that realizes high flux.
SUMMERY OF THE UTILITY MODEL
The utility model provides a high flux unicellular electrospray mass spectrum device has compensatied above-mentioned prior art not enough, can realize that the sequencing of cell gets into the mass spectrograph and carries out the analysis, with the help of the quick analysis ability of mass spectrograph and the ability of ten thousand kinds of materials in the single detectable, can realize quick unicellular analysis, does not need the mark of metal or dyestuff simultaneously.
The utility model provides a technical scheme that its technical problem adopted is:
a first long straight capillary spray needle, a second long straight capillary spray needle and a third long straight capillary spray needle are horizontally and fixedly connected in the support frame, a sample channel is formed in the horizontal direction, the left end of the first long straight capillary spray needle extends out of the sample channel, the right end of the first long straight capillary spray needle is arranged in the sample channel and close to the right end of the first three-way pipe, a clamping device for connecting the two three-way pipes is arranged between the left end of the second three-way pipe and the left end of the first three-way pipe, the first three-way pipe is connected with an injection pump through a pipeline, and a mass spectrometer is arranged on the right side of the third long straight capillary spray needle.
As a preferred technical scheme, the inner diameter of the first long straight capillary spray needle is 50-100 mu m, the outer diameter of the first long straight capillary spray needle is not more than 150 mu m, an inlet is arranged in the second three-way pipe, and the distance between the inlet and the right end of the horizontal pipe of the second three-way pipe is 1-3 cm;
as a preferred technical scheme, the second long straight capillary spray needle is sleeved outside the first long straight capillary spray needle, the inner diameter is 250 mu m, the outer diameter is 450 mu m, the left end of the second long straight capillary spray needle is arranged in the middle of the first three-way pipe, and the right end of the second long straight capillary spray needle penetrates out of the right end of the second three-way pipe by 1-2cm and is 2mm away from the spray opening end of the third long straight capillary spray needle.
According to a preferable technical scheme, the third long straight capillary spray needle is sleeved outside the second long straight capillary spray needle, the inner diameter is 580 mu m, and the caliber of the tip is 10 mu m.
As a preferable technical scheme, a high-voltage power supply is arranged close to the outer wall of the third long straight capillary needle.
According to the preferable technical scheme, the right end of the horizontal pipe of the first three-way pipe is sealed by a second graphite pad, a through hole is formed in the second graphite pad, and the right end of the second long straight capillary spray needle penetrates out of the through hole.
According to the preferable technical scheme, the first three-way pipe and the second three-way pipe respectively comprise a horizontal pipe and a vertical pipe, the left end of the horizontal pipe of the first three-way pipe is sealed by a first graphite pad, the right end of the horizontal pipe of the first three-way pipe is connected with the left end of the horizontal pipe of the second three-way pipe, a through hole is formed in the first graphite pad, and the right end of the first long straight capillary spray needle penetrates out of the through hole.
As a preferable technical solution, the method is characterized in that: the vertical pipe of the first three-way pipe is connected with the injection pump through a pipeline, and the vertical pipe of the second three-way pipe is connected with the waste liquid bottle through a pipeline.
As a preferable technical solution, the method is characterized in that: the right end of the horizontal pipe of the second three-way pipe is sealed by a third graphite pad, a through hole is formed in the third graphite pad, and the right end of the third long straight capillary spray needle penetrates out of the through hole.
Another technical scheme of the utility model is to utilize the utility model discloses a method that high flux unicellular electrospray mass spectrum device detected, include:
the method comprises the following steps: and (3) injecting the suspension cells washed twice by using the PBS solution into a first long straight capillary nozzle needle through a micro-injection pump, starting the injection pump, injecting the sheath fluid extracting agent into a first three-way pipe through the micro-injection pump, and applying a pulse high voltage of about 4KV to the first three-way pipe. The sheath liquid extractant flows to the right end of the first long straight capillary spray needle through the crack of the first long straight capillary spray needle and the second long straight capillary spray needle, and then is mixed with the cell suspension flowing out of the first long straight capillary spray needle.
Step two: the mixed liquid is sprayed out from the right end of the third long straight capillary spray needle 12, is ionized under the action of a high-voltage power supply electric field, enters a mass spectrometer for analysis, and the liquid which is not sprayed out flows out from the second three-way pipe.
As a preferable technical scheme, the sheath liquid extracting agent is selected from acetonitrile/water with a volume ratio of 2:1 and acetonitrile/water with 0.5 percent of acetic acid with a volume ratio of 2: 1.
Through above technical scheme, for prior art, the utility model discloses following beneficial effect has:
1. the utility model discloses a contain the long straight capillary nozzle needle of three-layer in two three-way pipes, the one deck capillary nozzle needle of the innermost is used for injecting into the cell suspension, the second floor capillary nozzle needle is used for injecting into sheath liquid extraction agent/sheath liquid, this liquid forms the double-layered stream on the one hand and makes the cell arrange along fluid center list, on the other hand provides ionization reagent for the ionization, outermost capillary nozzle needle provides the size condition of the required electric field of nanoliter electric spray and spray outlet requirement, and get rid of unnecessary liquid through the second tee bend, guarantee the spraying effect.
2. The utility model discloses with the help of the quick analysis ability of mass spectrum and the ability of the tens of thousands of materials of single detectable, can realize quick unicellular analysis, do not need the mark of metal or dyestuff simultaneously.
Drawings
The present invention will be further explained with reference to the drawings and examples.
FIG. 1 is a schematic structural diagram of a preferred embodiment 1 of the high throughput single-cell electrospray mass spectrometry apparatus of the present invention;
FIG. 2 is a schematic diagram of the PCA differentiation obtained after the single cell analysis of A549, HEPG2, HL60, K562 and PC12 cells by using the embodiment 1 of the present invention
In the figure:
1-a first long straight capillary spray needle, 2-a first graphite pad, 3-a support frame, 4-a first three-way pipe, 5-an injection pump, 6-a clamping device, 7-a second graphite pad, 8-a second long straight capillary spray needle, 9-a second three-way pipe, 10-a third graphite pad, 11-a waste liquid bottle, 12-a second long straight capillary spray needle, 13-a high-voltage power supply and 14-a mass spectrometer.
Detailed Description
The present invention will now be described in further detail with reference to the accompanying drawings. These drawings are simplified schematic drawings and illustrate the basic structure of the present invention only in a schematic manner, and thus show only the components related to the present invention.
Example 1
As shown in fig. 1, the utility model discloses a high flux unicellular electrospray mass spectrometry device includes support frame 3, horizontal fastening connection has first three-way pipe 4 and the second three-way pipe 9 of series connection in the support frame, form the sample passageway on the horizontal direction, be provided with first long straight capillary spray needle 1, the long straight capillary spray needle of second 8, the long straight capillary spray needle of third 12 on the sample passageway, the left end of first long straight capillary spray needle 1 stretches out outside the sample passageway, the right-hand member setting is in the sample passageway, be close to the right-hand member of first three-way pipe 4, and set up the clamping device 6 of connecting two three-way pipes between the left end of second three-way pipe 9, first three-way pipe 4 passes through the pipeline and links to each other with injection pump 5 third long straight capillary spray needle 12 right sides is provided with mass spectrograph 14.
The following are preferred embodiments:
the first long straight capillary spray needle 1, the second long straight capillary spray needle 8 and the third long straight capillary spray needle 12 are all made of fused quartz glass, the cross sections of the first long straight capillary spray needle, the second long straight capillary spray needle and the third long straight capillary spray needle are circular, and the first long straight capillary spray needle, the second long straight capillary spray needle and the third long straight capillary spray needle all comprise a tube body, an inlet. The inner diameter of the first long straight capillary spray needle 1 is 50-100 mu m, the outer diameter of the first long straight capillary spray needle is not more than 150 mu m, the inlet is arranged in the second three-way pipe 9, and the distance between the inlet and the right end of the horizontal pipe of the second three-way pipe 9 is 1-3 cm; the second long straight capillary spray needle 8 is sleeved outside the first long straight capillary spray needle 1, the inner diameter is 250 mu m, the outer diameter is 450 mu m, the left end of the second long straight capillary spray needle is arranged in the middle of the first three-way pipe 4, and the right end of the second long straight capillary spray needle penetrates out of the right end of the second three-way pipe 9 by 1-2cm and is 2mm away from the spray opening end of the third long straight capillary spray needle 12. The third long straight capillary spray needle 12 is sleeved outside the second long straight capillary spray needle 8, the inner diameter is 580 mu m, and the caliber of the tip end is 10 mu m. And a high-voltage power supply 13 is arranged close to the outer wall of the third long straight capillary needle 12. The right end of the horizontal pipe of the first three-way pipe 4 is sealed by a second graphite pad 7, a through hole is formed in the second graphite pad 7, and an outlet of the second long straight capillary spray needle 8 penetrates out of the through hole.
The first three-way pipe 4 and the second three-way pipe 9 both comprise a horizontal pipe and a vertical pipe, the left end of the horizontal pipe of the first three-way pipe 4 is sealed by a first graphite pad 2, the right end of the horizontal pipe of the first three-way pipe 4 is connected with the left end of the horizontal pipe of the second three-way pipe 2, a through hole is formed in the first graphite pad 2, and the right end of the first long straight capillary spray needle 1 penetrates out of the through hole. The vertical pipe of the first three-way pipe 4 is connected with the injection pump 5 through a pipeline, and the vertical pipe of the second three-way pipe 9 is connected with the waste liquid bottle 11 through a pipeline. The right end of the horizontal pipe of the second three-way pipe 9 is sealed by a third graphite pad 10, a through hole is formed in the third graphite pad 10, and the right end of the third long straight capillary spray needle 12 penetrates out of the through hole.
The utility model discloses an embodiment 1 high flux unicellular electrospray mass spectrum device detects's theory of operation is: and (3) injecting the suspension cells washed twice by using the PBS solution into the first long straight capillary spray needle 1 through a micro injection pump at a flow rate of about 2 muL/min, injecting a sheath fluid extraction agent through an injection pump 5 through the micro injection pump at a flow rate of 82 muL/min, and applying an electrostatic field high voltage of about 4KV to the sheath fluid extraction agent. The sheath liquid extractant flows to the right end of the first long straight capillary spray needle 1 through the crack of the first long straight capillary spray needle 1 and the second long straight capillary spray needle 8, and then is mixed with the cell suspension flowing out of the first long straight capillary spray needle. Because the flow rate of the sheath liquid extracting agent is high, the cell suspension flowing out of the first long straight capillary spray needle 1 can be compressed and stretched to enable cells in the liquid to be arranged in a row, then electrospray is realized through the right end of the third long straight capillary spray needle 12, suspended cells are broken and ionized under the action of high voltage 13, and effective molecular information is obtained through mass spectrometry.
The sheath liquid extractant may be mixed at different ratios using solvents such as methanol/water/acetonitrile, and acetonitrile/water (V: V =2: 1) containing 0.5% acetic acid may be used in the positive ion mode and acetonitrile/water (V: V =2: 1) may be used in the negative ion mode, depending on the test requirements.
The voltage of the high-voltage power supply 13 is 3500V, the flow rate of the cell suspension cleaned by the PBS solution is 2 microliter per minute, the flow rate of the sheath solution is 8 microliter per minute, and the frequency of the additional voltage is 370Hz, so that the flow rate of the cell suspension can be ensured to obtain good signals by mass spectrometry from 2-85 microliter per minute. The excessive solution caused by the over-fast flow rate is reversely led out by the second three-way pipe and collected into the waste liquid bottle, the good electrospray can be realized by the outlet with the diameter of 10 microns of the third long straight capillary spray needle 12, the cells are pulled to the spray outlet by the pulling force generated by the electrospray in the central shaft of the whole liquid flow, the cells are broken by the voltage at the spray outlet, and the molecules in the cells are ionized.
Adopt the utility model discloses a high throughput unicellular electrospray mass spectrometry device carries out unicellular analysis to A549, HEPG2, HL60, K562, PC12 cell, and MATLAB software processing experimental data carries out categorised differentiation through the PCA method and obtains PCA and distinguishes the picture, as shown in FIG. 2. The utility model discloses a high throughput unicellular electrospray mass spectrum device can realize the differentiation of different kinds of unicells, is applicable to the analysis of metabolite and lipid in the living cell to realize quick unicellular analysis, do not need the mark of metal or dyestuff simultaneously.
In light of the foregoing, it is to be understood that various changes and modifications may be made by those skilled in the art without departing from the spirit and scope of the invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (9)
1. A high-throughput single-cell electrospray mass spectrometry device, comprising: the sample injection device comprises a support frame (3), wherein a first three-way pipe (4) and a second three-way pipe (9) which are connected in series are horizontally and tightly connected in the support frame, a sample channel is formed in the horizontal direction, a first long straight capillary needle (1), a second long straight capillary needle (8) and a third long straight capillary needle (12) are arranged on the sample channel, the left end of the first long straight capillary needle (1) extends out of the sample channel, the right end of the first long straight capillary needle is arranged in the sample channel and close to the right end of the first three-way pipe (4), a clamping device (6) for connecting the two three-way pipes is arranged between the left end of the second three-way pipe (9), the first three-way pipe (4) is connected with an injection pump (5) through a pipeline, and a mass spectrometer (14) is arranged on the right side of the third long straight capillary needle (12).
2. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 1, wherein: the inner diameter of the first long straight capillary spray needle (1) is 50-100 micrometers, the outer diameter of the first long straight capillary spray needle is not more than 150 micrometers, the inlet is arranged in the second three-way pipe (9), and the distance between the inlet and the right end of the horizontal pipe of the second three-way pipe (9) is 1-3 cm.
3. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 1, wherein: the second long straight capillary spray needle (8) is sleeved outside the first long straight capillary spray needle (1), the inner diameter is 250 micrometers, the outer diameter is 450 micrometers, the left end of the second long straight capillary spray needle is arranged in the middle of the first three-way pipe (4), the right end of the second long straight capillary spray needle penetrates through the right end of the second three-way pipe (9) by 1-2cm, and the distance from the spray opening end of the third long straight capillary spray needle (12) is 2 mm.
4. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 1, wherein: the third long straight capillary spray needle (12) is sleeved outside the second long straight capillary spray needle (8), the inner diameter is 580 mu m, and the caliber of the tip end is 10 mu m.
5. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 1, wherein: and a high-voltage power supply (13) is arranged close to the outer wall of the third long straight capillary spray needle (12).
6. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 1, wherein: the right end of the horizontal pipe of the first three-way pipe (4) is sealed by a second graphite pad (7), a through hole is formed in the second graphite pad (7), and the right end of the second long straight capillary spray needle (8) penetrates out of the through hole.
7. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 1, wherein: the first three-way pipe (4) and the second three-way pipe (9) both comprise a horizontal pipe and a vertical pipe, the left end of the horizontal pipe of the first three-way pipe (4) is sealed by a first graphite pad (2), the right end of the horizontal pipe of the first three-way pipe (4) is connected with the left end of the horizontal pipe of the second three-way pipe (9), a through hole is formed in the first graphite pad (2), and the right end of the first long straight capillary spray needle (1) penetrates out of the through hole.
8. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 7, wherein: the vertical pipe of the first three-way pipe (4) is connected with the injection pump (5) through a pipeline, and the vertical pipe of the second three-way pipe (9) is connected with the waste liquid bottle (11) through a pipeline.
9. The high throughput single-cell electrospray mass spectrometry apparatus according to claim 6, wherein: the right end of the horizontal pipe of the second three-way pipe (9) is sealed by a third graphite pad (10), a through hole is formed in the third graphite pad (10), and the right end of the third long straight capillary spray needle (12) penetrates out of the through hole.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201920340599.1U CN209822593U (en) | 2019-03-18 | 2019-03-18 | High-throughput single-cell electrospray mass spectrometry device |
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| CN201920340599.1U CN209822593U (en) | 2019-03-18 | 2019-03-18 | High-throughput single-cell electrospray mass spectrometry device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109950126A (en) * | 2019-03-18 | 2019-06-28 | 上海慧多信息科技发展有限公司 | A kind of unicellular electrospray ionization mass spectrum device of high throughput and its detection method |
| US11651945B2 (en) | 2020-09-25 | 2023-05-16 | Ut-Battelle, Llc | Rapid droplet introduction interface (RDII) for mass spectrometry |
-
2019
- 2019-03-18 CN CN201920340599.1U patent/CN209822593U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109950126A (en) * | 2019-03-18 | 2019-06-28 | 上海慧多信息科技发展有限公司 | A kind of unicellular electrospray ionization mass spectrum device of high throughput and its detection method |
| US11651945B2 (en) | 2020-09-25 | 2023-05-16 | Ut-Battelle, Llc | Rapid droplet introduction interface (RDII) for mass spectrometry |
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