CN111213655A - Application of linalool oxide in attracting spodoptera frugiperda and composition containing linalool oxide - Google Patents
Application of linalool oxide in attracting spodoptera frugiperda and composition containing linalool oxide Download PDFInfo
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- CN111213655A CN111213655A CN202010051273.4A CN202010051273A CN111213655A CN 111213655 A CN111213655 A CN 111213655A CN 202010051273 A CN202010051273 A CN 202010051273A CN 111213655 A CN111213655 A CN 111213655A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/20—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom three- or four-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
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Abstract
The invention relates to application of linalool oxide in attracting spodoptera frugiperda and a composition containing linalool oxide. The composition comprises linalool oxide, a solvent, a light stabilizer and an antioxidant.
Description
Technical Field
The invention relates to application of linalool oxide in attracting spodoptera frugiperda.
Background
Spodoptera frugiperda originates in Central and south America and later spreads to North America. In 2016, month 1, the gene is found in Nigeria of West Africa for the first time, and because of no natural enemy and strong self-reproduction and migration capacity, 44 African countries in the south of Saharan are rolled up within two years. In 2018, Spodoptera frugiperda diffused into Asia and rapidly diffused in India, Srilanca, Bengal, Thailand, Myanmar, Vietnam, Laos in south and southeast Asia.
The Spodoptera frugiperda has strong reproductive capacity, one female can lay eggs by about 1000 eggs on average, and 2000 eggs can be produced at most; the host is wide, and the 353 plants of 75 families can be eaten; the migration and flying are fast, and adults can migrate more than 100 kilometers at one night by means of atmospheric circulation; the fast-eating property and the large food consumption of 5-6 instar larvae cause that Spodoptera frugiperda is easy to burst into disasters.
In 1 month of 2019, Spodoptera frugiperda was found in Yunnan of China for the first time, and then the Spodoptera frugiperda spread to 26 provinces, cities and autonomous regions in southwest, south China, the middle and lower reaches of Yangtze river, Huang-Huai-river, northwest China and North China by 10 months of 2019.
At present, chemical control is still the main control means of spodoptera frugiperda, which causes that spodoptera frugiperda has different degrees of drug resistance to various types of insecticides, and meanwhile, chemical control also has obvious influence on ecological environment, so that the search for green and pollution-free control methods is urgent. The components of the sex attractant mainly comprise Z11-16: Ac, Z9-14: Ac, 14: Ac and Z11-14: Ac. Although the use of sex attractant is one of the means for measuring and controlling at present, the sex attractant can only trap a part of male insects, the male insects which are not trapped can make female insects lay eggs through multiple mating, and the eggs laying are harmful to the female insects, so the sex attractant can play the role of measuring and reducing the insect quantity, but is not very effective as a control means.
If a chemical substance that attracts both males and females, or only females, is developed, it can be one of the more effective means of controlling spodoptera frugiperda than a sex attractant.
Disclosure of Invention
The invention provides application of linalool oxide in luring spodoptera frugiperda.
The second invention provides an attractant composition, which comprises linalool oxide, a light stabilizer, an antioxidant and a solvent.
In one embodiment, the solvent is selected from at least one of n-hexane, natural vegetable oil, dioctyl terephthalate, and methylene chloride.
In one embodiment, the light stabilizer is selected from at least one of the group consisting of salicylates, benzophenones, benzotriazoles, substituted acrylonitriles, triazines, and hindered amines.
In one embodiment, the light stabilizer is methyl salicylate.
In one embodiment, the antioxidant is selected from at least one of vitamin E, 2, 6-di-tert-butyl-4-methylphenol, 2, 5-di-tert-butylhydroquinone, and antioxidant 168.
In one embodiment, the linalool oxide is present in an amount ranging from 1% to 20% by volume of the total composition taken as 100%; the content of the light stabilizer is 1 to 10 percent; the content of the antioxidant is 1 to 10 percent; the content of the solvent is 64% to 97%. Wherein, when the antioxidant is in the form of a solid, its content refers to the mass (antioxidant)/volume (composition) content.
In one embodiment, the linalool oxide is present in an amount ranging from 1% to 10% by volume of the total composition taken as 100%; the content of the light stabilizer is 1 to 10 percent; the content of the antioxidant is 1 to 10 percent; the content of the solvent is 70% to 97%. Wherein, when the antioxidant is in the form of a solid, its content refers to the mass (antioxidant)/volume (composition) content.
In one embodiment, the linalool oxide is present in an amount ranging from 4% to 10% by volume of the total composition taken as 100%; the content of the light stabilizer is 3-6%; the content of the antioxidant is 3-10%; the content of the solvent is 74 to 90 percent. Wherein, when the antioxidant is in the form of a solid, its content refers to the mass (antioxidant)/volume (composition) content.
The invention also provides a lure comprising a carrier and the composition of any one of the two aspects of the invention.
In one embodiment, the carrier is selected from at least one of natural rubber, synthetic rubber, polyethylene delay tube, polyvinyl chloride delay tube, resin and viscose material.
In a specific embodiment, the amount of the linalool oxide in the lure is 0.1 to 10mg per lure, and preferably, the amount of the linalool oxide in the lure is 1 to 10mg per lure.
In a specific embodiment, the amount of linalool oxide in the lure is from 4 to 5mg per lure.
In one embodiment, the prepared lure is sealed in a plastic bag and stored at a temperature below 4 ℃.
The invention has the beneficial effects that:
spodoptera frugiperda is a new invasive species in China, and chemical control is mainly used at the present stage, but Spodoptera frugiperda has strong drug resistance to organophosphorus, organochlorine and pyrethroid medicaments in the origin, so that the development of an effective and environment-friendly control technology is the key for controlling the pest. Although the use of sex attractant is one of the means for measuring and controlling at present, the sex attractant can only trap a part of male insects, the male insects which are not trapped can make female insects lay eggs through multiple mating, and the eggs laying are harmful to the female insects, so the sex attractant can play the role of measuring and reducing the insect quantity, but is not very effective as a control means. The linalool oxide developed by the invention can attract male insects and female insects, so that the linalool oxide can be used as one of measures for more effectively preventing and controlling spodoptera frugiperda compared with a sex attractant.
Drawings
FIG. 1 shows the Y-type tube behavior response of E.spodoptera females to varying concentrations of linalool oxide.
FIG. 2 shows the Y-type tube behavior response of Male Spodoptera frugiperda to varying concentrations of linalool oxide.
FIG. 3 shows the Y-type tube behavior of female Spodoptera frugiperda for the dissolution of linalool oxide in different solvents
FIG. 4 shows the relative EAG response values of E.spodoptera to linalool oxide.
FIG. 5 shows the relative EAG response of Male Spodoptera frugiperda to linalool oxide.
Figure 6 shows the trapping amount of linalool oxide at different concentrations in the lure per day.
Figure 7 shows the percentage of the female insects trapped by linalool oxide at different concentrations in the trap core.
Detailed Description
The above-described aspects of the invention are explained in more detail below by means of preferred embodiments, but they are not intended to limit the invention.
The reagents in the examples of the present invention were all commercially available unless otherwise specified.
Example 1
Y-tube behavioral response
The experiment is carried out under the natural light environment with the room temperature of 24 +/-1 ℃ and the relative humidity of 40 +/-5 percent. Before the experiment, the Y-type olfactometer (Y-type tube) is washed by acetone and n-hexane in sequence. Preparing linalool oxide into samples to be detected with concentration gradients of 0.1 mu g/mu l, 1 mu g/mu l and 10 mu g/mu l by using n-hexane, taking 10 mu l of the samples to be detected and the n-hexane as a reference, respectively dripping the samples to be detected and the n-hexane on two different pieces of filter paper, respectively putting the two pieces of filter paper into odor source device bottles on two side walls of a Y-shaped pipe, opening an atmosphere sampler, enabling the flow rate of air flow in the Y-shaped pipe to be 100ml/min, then putting the Spodoptera frugiperda adults within 1 day of eclosion into a release pipe, observing for 5min, and considering that the odor sources are selected when one arm of the Spodoptera frugiperda is selected for the first time to exceed 5cm and the duration to exceed 30 s. Each test insect was used only 1 time, each treated adult insect was of the same sex and at least 30, and 5 of each test were washed and arm-changed (odor source and control arm were exchanged) to eliminate errors caused by asymmetry or orientation of the two arms of the Y-tube.
The behavioral response results of the Y-shaped tube are shown in figures 1 and 2, and chi-square test results show that 1 mug/mul of linalool oxide has a remarkable attraction effect on female insects, the attraction effects of the other two concentrations are not remarkable, and 10 mug/mul of linalool oxide has a remarkable avoidance effect on male insects.
Example 2
Y-tube behavioral response
Linalool oxide was formulated with n-hexane, natural vegetable oil, dioctyl terephthalate and dichloromethane to give 1. mu.g/. mu.l of sample to be tested, with the respective solvents as controls.
The other operations were the same as in example 1.
The behavioral reaction results of the Y-shaped tube are shown in figure 3, and the chi-square test results show that the linalool oxide dissolved by a plurality of solvents has a remarkable attraction effect on the female insects.
Example 3
Relative reaction value of EAG
The electrophysiological reaction of male and female spodoptera frugiperda adults with linalool oxide at different concentrations was determined using EAG (Syntech, Germany). The specific operation steps are as follows: the linalool oxide is prepared into samples to be detected with 3 concentration gradients of 0.1 mu g/mu l, 1 mu g/mu l and 10 mu g/mu l by using n-hexane, 10 mu l of the samples to be detected or the control is dripped on filter paper each time by using the n-hexane as a control, and then the filter paper is put into a sample tube. The tentacles of spodoptera frugiperda adults were cut from the base and a small portion (about 1mm) of the end of the tentacles was cut off and the two ends of the tentacles were glued to the fork electrodes using conductive glue, respectively. The air flow at the contact angle was adjusted to 5 ml/s. During testing, the continuous air flow is switched to the stimulation air flow of the sample to be tested, namely the continuous air flow is suspended, the stimulation air flow of the switched sample to be tested is 1ml/s, and the duration time of the stimulation air flow of the sample to be tested is 0.5 s. The resulting electrophysiological signals were recorded with Syntech software. The same dose of linalool oxide was stimulated 1 time on 1 antennal, 10 antennals per treatment, repeated three times.
The EAG results are shown in figures 4 and 5, and the results show that the electrophysiological response value of the spodoptera frugiperda female to 1 mu g/mu l of linalool oxide is the highest, the electrophysiological response values of the female under the stimulation of the linalool oxide with three concentrations are higher than those of the male, and the electrophysiological response value of the male to 10 mu g/mu l of linalool oxide with the other two concentrations is higher.
Example 4
Trapping in field
Test site: in the Yangkcun corn land along Yangtze river from east to the Yangli town of the Yanghui province, the autumn corn is planted in the area in a connected mode, the area of the corn planted in the test land is about 500 mu, the spodoptera frugiperda on the grassland is the serious pest, and the pest rate is more than 90%.
The test method comprises the following steps: preparing 4 components of linalool oxide, n-hexane, methyl salicylate and vitamin E according to the volume percentage of table 1 (orthogonal experimental table is designed according to 3 factors and 4 levels, and the n-hexane is used for supplementing 100%) to obtain an attractant solution, penetrating 100 mu l of the attractant solution into a latex plug (a lure core), and then placing the latex plug into a covered funnel type trap. The traps are spaced by 20 meters, the height from the ground is 1.5 meters, a random block design is adopted, the treatment is repeated three times, and the pest trapping quantity of each trap is counted after 3 days.
TABLE 1
Wherein, K1、K2、K3And K4Representing 4 levels of 3 factors, respectively.
According to the results in table 1, the optimal percentage is 4% of linalool oxide, 3% of methyl salicylate, 3% of vitamin E and the balance of n-hexane to 100%; secondly, linalool oxide 10%, methyl salicylate 6%, vitamin E10%, and n-hexane to make up to 100%; and linalool oxide 1%, methyl salicylate 1% or 10% (equivalent), vitamin E1% or 6% (equivalent), and n-hexane to make up 100%. Thus, the range of formulations is: linalool oxide 1-10%, methyl salicylate 1-10%, vitamin E1-10%, and n-hexane to make up 100%; the preferred formulation ranges are: linalool oxide 4-10%, methyl salicylate 3-6%, vitamin E3-10%, and n-hexane to make up 100%.
Example 5
Trapping in field
Test site: in the Yangkcun corn land along Yangtze river from east to the Yangli town of the Yanghui province, the autumn corn is planted in the area in a connected mode, the area of the corn planted in the test land is about 500 mu, the spodoptera frugiperda on the grassland is the serious pest, and the pest rate is more than 90%.
The test method comprises the following steps: the linalool oxide, n-hexane, methyl salicylate and vitamin E4 are mixed according to the volume percentage of 4%, 90%, 3% and 3% to obtain the attractant solution. The attractant solution is permeated into a latex plug (attracting core), and the latex plug is placed in a funnel-type trapper with a cover, so that the amount of linalool oxide on each attracting core in each trapper is 0.1, 0.5, 1.0, 5.0 and 10.0mg, and n-hexane is used as a control. The traps are spaced by 20 meters and are 1.5 meters away from the ground, a random block design is adopted, the number of the attracted insects and the number of the female insects of each trap are counted in the morning every day, Spodoptera frugiperda lurea trapped by the traps is eliminated, and the investigation is continued for 10 days. Each treatment was repeated three times. The results of total number of insects trapped per day are shown in FIG. 6. The percentage of female insects trapped in the trapping core for 10 days under different linalool oxide dosages is calculated, and the result is shown in figure 7.
Fig. 6 and 7 verify the effect of linalool oxide in different doses in the field under the optimal proportioning of orthogonal experiments, and simultaneously show that 5mg of linalool oxide in five dosages in the lure is optimal, and 1mg and 10mg are next, but all the tested concentrations are obviously superior to the n-hexane control.
Claims (10)
1. The application of linalool oxide in luring spodoptera frugiperda.
2. An attractant composition comprising linalool oxide, a light stabilizer, an antioxidant and a solvent.
3. The composition of claim 2, wherein the solvent is selected from at least one of n-hexane, natural vegetable oil, dioctyl terephthalate, and methylene chloride.
4. A composition according to claim 2 or 3, characterized in that the light stabilizer is selected from at least one of the group consisting of salicylates, benzophenones, benzotriazoles, substituted acrylonitriles, triazines and hindered amines.
5. A composition according to claim 2 or 3, characterized in that the light stabilizer is methyl salicylate.
6. The composition according to any one of claims 2 to 5, wherein the antioxidant is at least one selected from the group consisting of vitamin E, 2, 6-di-tert-butyl-4-methylphenol, 2, 5-di-tert-butylhydroquinone and antioxidant 168.
7. The composition according to any one of claims 2 to 6, wherein the linalool oxide is contained in an amount ranging from 1% to 20% by volume, taken as 100% by volume of the total composition; the content of the light stabilizer is 1 to 10 percent; the content of the antioxidant is 1 to 10 percent; the content of the solvent is 64% to 97%;
preferably, the linalool oxide is contained in an amount ranging from 1% to 10% by taking the total volume of the composition as 100%; the content of the light stabilizer is 1 to 10 percent; the content of the antioxidant is 1 to 10 percent; the content of the solvent is 70% to 97%;
preferably, the linalool oxide is contained in an amount ranging from 4% to 10% by taking the total volume of the composition as 100%; the content of the light stabilizer is 3-6%; the content of the antioxidant is 3-10%; the content of the solvent is 74 to 90 percent.
8. A lure comprising a carrier and a composition according to any one of claims 2 to 7.
9. The lure according to claim 8, wherein the carrier is selected from at least one of natural rubber, synthetic rubber, polyethylene slow release tube, polyvinyl chloride slow release tube, resin, and viscose material.
10. The lure according to claim 8 or 9, wherein the amount of linalool oxide in the lure is 0.1 to 10mg per lure;
preferably, the amount of the linalool oxide in the lure is 1 to 10 mg/lure;
preferably, the amount of linalool oxide in the lure is 4 to 5mg per lure.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331201A (en) * | 2021-06-03 | 2021-09-03 | 中国农业科学院植物保护研究所 | Attractant composition for preventing and treating spodoptera frugiperda and application thereof |
| CN116806575A (en) * | 2023-06-30 | 2023-09-29 | 浙江大学 | Method for improving defensive hormone in second-generation crops and application thereof |
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| CN109965088A (en) * | 2019-03-01 | 2019-07-05 | 河南省农业科学院植物保护研究所 | Spodopterafrugiperda larva artificial feed, preparation method and application |
| CN110074107A (en) * | 2019-05-14 | 2019-08-02 | 中国农业科学院植物保护研究所 | A kind of Spodopterafrugiperda attractant composition and its application |
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| CN109965088A (en) * | 2019-03-01 | 2019-07-05 | 河南省农业科学院植物保护研究所 | Spodopterafrugiperda larva artificial feed, preparation method and application |
| CN110074107A (en) * | 2019-05-14 | 2019-08-02 | 中国农业科学院植物保护研究所 | A kind of Spodopterafrugiperda attractant composition and its application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113331201A (en) * | 2021-06-03 | 2021-09-03 | 中国农业科学院植物保护研究所 | Attractant composition for preventing and treating spodoptera frugiperda and application thereof |
| CN116806575A (en) * | 2023-06-30 | 2023-09-29 | 浙江大学 | Method for improving defensive hormone in second-generation crops and application thereof |
| CN116806575B (en) * | 2023-06-30 | 2024-04-26 | 浙江大学 | Method for improving defensive hormone in second-generation crops and application thereof |
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