CN1112022A - High immune serum for prevention of aerohydrophilic monadosis and its preparation method - Google Patents
High immune serum for prevention of aerohydrophilic monadosis and its preparation method Download PDFInfo
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- CN1112022A CN1112022A CN 94105069 CN94105069A CN1112022A CN 1112022 A CN1112022 A CN 1112022A CN 94105069 CN94105069 CN 94105069 CN 94105069 A CN94105069 A CN 94105069A CN 1112022 A CN1112022 A CN 1112022A
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- aeromonas hydrophila
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Abstract
The high-immunity serum for curing aero-hydrophilic monadosis of soft-shelled turtle is prepared with the serum of animal having immunity from aero-hydrophilic monadosis (100 ml), thiomerosal (1ml%) and gentamycin sulfate (240 thousands units) through extraction of bacterial strain, preparing antigen, animal immunizing, collecting blood preparing serum and dispensing, and features high curative effect and simple preparing process.
Description
The invention belongs to a kind of hyper-immune serum for the treatment of the Trionyx sinensis Wiegmann disease, particularly treating the Trionyx sinensis Wiegmann hueppe's disease has good effect.
Trionyx sinensis Wiegmann claims Trionyx sinensis Wiegmann again.Its meat is nutritious, delicious flavour, high protein, low fat, for taking a tonic or nourishing food to build up one's health the high tonic of body-building, artificial cultivation can bring high economic benefit, the artificial feeding Trionyx sinensis Wiegmann, and what harm was maximum is the interference of disease, Trionyx sinensis Wiegmann has infectious disease (mainly being aeromonas hydrophila disease), parasitic disease, mycosis, metabolic disease etc., what current harm was the most serious is to fester in the sick Trionyx sinensis Wiegmann of aeromonas hydrophila disease back, blush appears in base plate, the swelling of head cervical region, hyperemia, eye is blind, mashed pawl even come off, each internal organs of internal organs are hemorrhage, congested, edema, present the deteriorated blood symptom, penicillin, the streptomycin treatment does not all prove effective this disease sickness rate height, infect fast, the mortality rate height, the disease of trick is stretched, so that whole pond Trionyx sinensis Wiegmann is all dead.
The purpose of this invention is to provide a kind of hyper-immune serum for the treatment of the Trionyx sinensis Wiegmann aeromonas hydrophila disease and preparation method thereof, be used for the treatment of the Trionyx sinensis Wiegmann aeromonas hydrophila disease, guarantee the sound development of artificial cultivation Trionyx sinensis Wiegmann.
Technical scheme of the present invention is achieved in that the hyper-immune serum that is used for the treatment of the Trionyx sinensis Wiegmann Aeromonas hydrophila, is made up of following material: the every 100mL of animal serum with Aeromonas hydrophila immunologic function adds the thimerosal of 1mL1% and the gentamycin sulfate of 240,000 units.
The preparation process that is used for the treatment of the hyper-immune serum of Trionyx sinensis Wiegmann Aeromonas hydrophila comprises the steps:
The extraction of a, strain.Selection has the sick Trionyx sinensis Wiegmann of aeromonas hydrophila disease symptom, dissects the disease Trionyx sinensis Wiegmann, breaks behind the taking-up liver, gets bacterium on pathological tissues, is seeded on the plating medium and cultivates;
B, antigenic preparation.The plating medium that covers with antibacterial is taken out from incubator, with normal saline, glass bead flushing plate face, heating washing liquid, the centrifugalize washing liquid, outwell top clear liquid, add an amount of normal saline and wash out precipitation, the thimerosal solution that every 100mL adds 1mL 1% is antigen;
C, give animal immune.Every rabbit injections of antigens 0.5mL to rabbit injection Aeromonas hydrophila aluminium hydroxide vaccine 1-1.5mL, injected Aeromonas hydrophila viable bacteria 1～1.5mL to rabbit after seven days after four days;
D, blood sampling, preparation serum.Kill rabbit after seven days, blood sampling, the centrifugal blood of adopting is made serum;
E, preparation.The thimerosal of adding 1mL 1% and the gentamycin sulfate of 240,000 units get final product in every 100mL serum.
The animal of immunity also can be a sheep, gives every sheep injections of antigens 2mL, gives every sheep injection Aeromonas hydrophila aluminium hydroxide vaccine 6mL after four days, gives every sheep injection Aeromonas hydrophila viable bacteria 6mL after seven days, just can take a blood sample preparation serum after seven days.
Medicine of the present invention detects through experiment, and effective percentage reaches more than 98%, is the best antimicrobial preparation of current treatment Trionyx sinensis Wiegmann disease, and the Trionyx sinensis Wiegmann aeromonas hydrophila disease is had special efficacy, can guarantee that manually supporting Trionyx sinensis Wiegmann already develops in a healthy way, and obtains huge social benefit and economic benefit.
Claims (4)
1, a kind of hyper-immune serum for the treatment of Aeromonas hydrophila, be made up of following material: the every 100mL of animal serum with Aeromonas hydrophila immunologic function adds the thimerosal of 1mL1% and the gentamycin sulfate of 240,000 units.
2, a kind of preparation method for the treatment of the hyper-immune serum of Aeromonas hydrophila comprises the steps:
The extraction of a, strain.Selection has the sick Trionyx sinensis Wiegmann of having a liking for water Zymomonas mobilis disease symptoms, dissects the disease Trionyx sinensis Wiegmann, breaks behind the taking-up liver, gets bacterium on pathological tissues, is seeded on the plating medium and cultivates;
B, antigenic preparation.The plating medium that covers with antibacterial is taken out from incubator, and with normal saline flushing plate face, heating washing liquid, the centrifugalize washing liquid is outwelled top clear liquid, adds an amount of normal saline and washes out precipitation, and the thimerosal solution that every 100mL solution adds 1mL 1% is antigen;
C, give animal immune.Give the animal injections of antigens, injection Aeromonas hydrophila aluminium hydroxide vaccine after four days, injection Aeromonas hydrophila viable bacteria after seven days;
D, blood sampling, preparation serum.Kill animal after seven days, blood sampling, the centrifugal blood of adopting is made serum;
E, preparation.The thimerosal of adding 1mL1% and the gentamycin sulfate of 240,000 units get final product in every 100mL serum.
3, preparation method according to claim 2, it is characterized in that immune employed animal is a rabbit, every rabbit injections of antigens 0.5mL, injection Aeromonas hydrophila aluminium hydroxide vaccine 1-1.5mL after four days, injection Aeromonas hydrophila Seedling 1～1.5mL alive after seven days.
4, preparation method according to claim 2 is characterized in that immune employed animal is a sheep, every sheep injections of antigens 2mL, injection Aeromonas hydrophila aluminium hydroxide vaccine 6mL after four days, injection Aeromonas hydrophila viable bacteria 6mL after seven days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94105069 CN1112022A (en) | 1994-05-16 | 1994-05-16 | High immune serum for prevention of aerohydrophilic monadosis and its preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94105069 CN1112022A (en) | 1994-05-16 | 1994-05-16 | High immune serum for prevention of aerohydrophilic monadosis and its preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1112022A true CN1112022A (en) | 1995-11-22 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94105069 Pending CN1112022A (en) | 1994-05-16 | 1994-05-16 | High immune serum for prevention of aerohydrophilic monadosis and its preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1112022A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292794C (en) * | 2003-08-22 | 2007-01-03 | 福建省农业科学院畜牧兽医研究所 | Preparation of immuno-stimulation composition for hemolycin in monad |
-
1994
- 1994-05-16 CN CN 94105069 patent/CN1112022A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292794C (en) * | 2003-08-22 | 2007-01-03 | 福建省农业科学院畜牧兽医研究所 | Preparation of immuno-stimulation composition for hemolycin in monad |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |