CN1112021A

CN1112021A - Aluminium hydroxide vaccine for prevention of aerohydrophilic monadosis and its preparation method

Info

Publication number: CN1112021A
Application number: CN 94105068
Authority: CN
Inventors: 虞蕴如; 赵保平
Original assignee: SPECIAL BREEDING DEV CO SHIJIAZHUANG CITY
Current assignee: SPECIAL BREEDING DEV CO SHIJIAZHUANG CITY
Priority date: 1994-05-16
Filing date: 1994-05-16
Publication date: 1995-11-22

Abstract

The method for preparing vaccine to prevent aero-hydrophilic monadosis of soft-shelled turtle that is, to prevent hemorrhagic septemia includes extraction of bacterial strain, culture, preparing nutritive broth, culture of seed broth, culture of productive broth, deactivating and preparing vaccine, and features simple process and obtained vaccine having obvious preventive effect.

Description

Aluminium hydroxide vaccine for prevention of aerohydrophilic monadosis and its preparation method

The invention belongs to a kind of vaccine that prevents the Trionyx sinensis Wiegmann disease, particularly prevent the vaccine and the preparation method of Trionyx sinensis Wiegmann hueppe's disease.

Trionyx sinensis Wiegmann, the formal name used at school Trionyx sinensis (Wiegmann).Its meat is nutritious, delicious flavour, and high protein, low fat, for taking a tonic or nourishing food to build up one's health the high tonic of body-building, artificial cultivation can bring high economic benefit.The artificial feeding Trionyx sinensis Wiegmann, what harm was maximum is the interference of disease.Trionyx sinensis Wiegmann has infectious disease (mainly being aeromonas hydrophila disease), parasitic disease, mycosis, metabolic disease etc., and what current harm was the most serious is aeromonas hydrophila disease.Fester in sick Trionyx sinensis Wiegmann back, blush appears in base plate, the swelling of head cervical region, hyperemia, and eye is blind, mashed pawl even come off, and hemorrhage, congested, the edema of each internal organs of internal organs presents the deteriorated blood symptom, and penicillin, streptomycin are treated and are not all proved effective.This disease sickness rate height infects soon, the mortality rate height, and the disease of trick is stretched, and can make whole pond Trionyx sinensis Wiegmann death.

The purpose of this invention is to provide a kind of vaccine that prevents the Trionyx sinensis Wiegmann aeromonas hydrophila disease and preparation method thereof, be used to prevent Trionyx sinensis Wiegmann to suffer from aeromonas hydrophila disease, guarantee the sound development of artificial cultivation Trionyx sinensis Wiegmann.

Technical scheme of the present invention is achieved in that the preparation of the vaccine of prevention Trionyx sinensis Wiegmann aeromonas hydrophila disease comprises the steps:

The extraction of a, strain.Selection has the sick Trionyx sinensis Wiegmann of aeromonas hydrophila disease symptom, dissects the disease Trionyx sinensis Wiegmann, breaks behind the taking-up liver, gets bacterium on pathological tissues, cultivates, and after 24 hours, identifies by bacteriology's classification method and Key program, is decided to be Aeromonas hydrophila;

B, spawn culture.It is strong to filter out virulence from Aeromonas hydrophila, and the typical bacterial strain of form, cultural character and biochemical reaction is a strain, is inoculated on the plating medium, cultivates in incubator;

C, preparation nutrient broth.In the 1000mL distilled water, add 5 gram sodium chloride, 5 gram beef extracts, 1 gram dipotassium hydrogen phosphate, 10 gram peptones, be heated to boiling, transparent, after cooling, add the 50mL new-born calf serum;

The cultivation of d, seed meat soup.Adding 5 colonies typicals in every 100mL nutrient broth makes seed meat soup and cultivates;

E, the cultivation of producing meat soup.Adding 1-2mL seed meat soup in every 100mL nutrient broth makes and produces meat soup and cultivate;

F, the deactivation of producing meat soup.With the formalin of the production meat soup adding 1mL36% after every 100mL cultivation, put into incubator and carry out deactivation;

The preparation of g, Aeromonas hydrophila aluminium hydroxide vaccine.Add gel aluminum hydroxide behind the 20ml autoclaving with the production meat soup of every 100ml deactivation, the limit edged shakes up, and allows its natural sedimentation, the clear liquid 20ml above treating to extract after the solution layering, and rest parts is Aeromonas hydrophila aluminium hydroxide vaccine.

The experiment proved that, inject in the Trionyx sinensis Wiegmann body behind this vaccine and all produced antibody that do not have the appearance of aeromonas hydrophila disease again, preventive effect is remarkable.

The temperature of spawn culture is 25～35 ℃, and the time is 24 hours.

The cultivation temperature of seed meat soup is 25～35 ℃, and the excellent time in age is 12～18 hours.

The cultivation temperature of producing meat soup is 25～35 ℃, and the time is 36～48 hours.

The deactivation incubator temperature of producing meat soup is 25～35 ℃, and the time is 24 hours.

Trionyx sinensis Wiegmann aeromonas hydrophila disease (hueppe's disease), be general antibiotics, the disease that is difficult to tackle, the development of this product is to utilize biotechnology to reach the purpose of prevention and treatment Trionyx sinensis Wiegmann disease, filling the domestic gaps, is the best antimicrobial preparation of breeding soft-shell turtle industry a few days ago, has tangible curative effect, can impel the breeding soft-shell turtle industry more to attain a new height, develop in a healthy way.

Claims

1, a kind of aluminium hydroxide vaccine for prevention of aerohydrophilic monadosis and preparation method comprise the steps:

A. the extraction of strain.Selection has the sick Trionyx sinensis Wiegmann of aeromonas hydrophila disease symptom, dissects the disease Trionyx sinensis Wiegmann, breaks behind the taking-up liver, gets bacterium on pathological tissues, cultivates;

B. spawn culture.It is strong to filter out virulence from Aeromonas hydrophila, and the typical bacterial strain of form, cultural character and biochemical reaction is a strain, is inoculated on the plating medium, cultivates in incubator;

C. prepare nutrient broth.In the 1000ml distilled water, add 5 gram sodium chloride, 5 gram beef extracts, 1 gram dipotassium hydrogen phosphate, 10 gram peptones, be heated to boiling, transparent, after cooling, add the 50ml new-born calf serum;

D. the cultivation of seed meat soup.Adding 5 colonies typicals in every 100ml nutrient broth makes seed meat soup and cultivates;

E. produce the cultivation of meat soup.Adding 1～2ml seed meat soup in every 100ml nutrient broth makes and produces meat soup and cultivate;

F. produce the deactivation of meat soup.With the formalin of the production meat soup adding 1ml36% after every 100ml cultivation, put into incubator and carry out deactivation;

G. the preparation of Aeromonas hydrophila aluminium hydroxide vaccine.Add the autoclaved gel aluminum hydroxide of 20ml with the production meat soup of every 100ml deactivation, shake up, allow its natural sedimentation, the clear liquid 20ml above treating to extract after the solution layering, the remaining Aeromonas hydrophila aluminium hydroxide vaccine that is.

2, preparation method according to claim 1, the temperature that it is characterized in that spawn culture is 25～35 ℃, the time is 24 hours.

3, preparation method according to claim 1, the cultivation temperature that it is characterized in that seed meat soup is 25～35 ℃, the excellent time in age is 12～18 hours.

4, preparation method according to claim 1, the cultivation temperature that it is characterized in that producing meat soup is 25～35 ℃, the time is 36～48 hours.

5, preparation method according to claim 1, the deactivation temperature that it is characterized in that producing meat soup is 25～35 ℃, the time is 24 hours.