CN111202066A - A nontoxic bait produced by coating technology and capable of affecting murine digestive system - Google Patents

A nontoxic bait produced by coating technology and capable of affecting murine digestive system Download PDF

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Publication number
CN111202066A
CN111202066A CN202010110776.4A CN202010110776A CN111202066A CN 111202066 A CN111202066 A CN 111202066A CN 202010110776 A CN202010110776 A CN 202010110776A CN 111202066 A CN111202066 A CN 111202066A
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bait
peroxide
acid
digestive system
affecting
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CN202010110776.4A
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CN111202066B (en
Inventor
张华伟
王佳建
卢洪斌
葛迎春
王春磊
王英爽
刘丽颖
韩冰
于海龙
林文华
樊殿佐
关丽萍
吴爽
宋显全
安允龙
刘金霞
李万龙
张少龙
宋久栋
朱遵山
胡明慧
孙黎明
栾媛
高俊峰
卫鑫
安永一
闫金
刘世一
魏金龙
陈保胜
朱遵福
孙志权
张鹏
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Heilongjiang Pingshan Pharmaceutical Factory Of Forestry
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Heilongjiang Pingshan Pharmaceutical Factory Of Forestry
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/002—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits
    • A01N25/004—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing a foodstuff as carrier or diluent, i.e. baits rodenticidal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/02—Sulfur; Selenium; Tellurium; Compounds thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/50—Feeding-stuffs specially adapted for particular animals for rodents

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
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  • Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
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  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
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Abstract

The invention discloses a nontoxic bait which is produced by using a coating technology and influences the digestive system of rats, and the preparation raw materials at least comprise organic acid, peroxide and bait; the organic acid and the peroxide are respectively coated by capsules. The bait adopts nontoxic raw materials, and the raw materials which easily cause the mice to refuse to eat and eat less are wrapped by capsules, after the mice eat the bait, the digestive function is destroyed, the blockage and obstruction appear in the intestinal tract after the mice eat the bait for many times, and then the death occurs, the acute poisoning phenomenon can not appear in the death process, the influence on other mice in the population can not be generated, the drug resistance can not be caused, the whole death rate is improved, meanwhile, the safety of animals except the mice is high, and the bait can be popularized and used in various fields of production, life and the like for a long time.

Description

A nontoxic bait produced by coating technology and capable of affecting murine digestive system
Technical Field
The invention relates to the field of rodent control, in particular to a non-toxic bait which is produced by using a coating technology and influences the digestive system of rodents.
Background
The mouse is one of the fastest breeding animals in mammals, has strong viability, is often exposed in bacteria-carrying places such as sewers, toilets and kitchens and moves back and forth with clean places, pathogenic bacteria are transmitted by mouse feet, body hair and stomach carriers, the mouse can exist in any place where people live, and sometimes the mouse bites an electric wire to cause fire; as omnivorous animals with a large population quantity, rats cause a great deal of grain loss every year, and therefore, rat control is particularly important.
In the prevention and treatment of the rats, chemical deratization accounts for a majority, the acute bait toxicity is too strong, the death time of the rats is short, the population alertness is easy to cause, and the overall deratization effect is poor; although the toxicity of chronic baits is reduced, rats have strong reproductive ability, and after many generations, the baits are resistant to toxicants, so that the drug property is poor, and the baits also have threats to animals except rats.
Disclosure of Invention
In order to solve the above problems, a first aspect of the present invention provides a nontoxic bait for affecting the digestive system of rodents produced using a coating technique, which is prepared from raw materials including at least an organic acid, a peroxide, and a bait; the organic acid and the peroxide are respectively coated by capsules.
As a preferable technical scheme, the preparation raw materials at least comprise 1-50 parts by weight of organic acid, 1-50 parts by weight of peroxide and 100-500 parts by weight of bait.
As a preferred technical scheme, the organic acid is selected from one or more of citric acid, malic acid, oxalic acid, succinic acid, tartaric acid, ascorbic acid, quinic acid, shikimic acid, benzoic acid, salicylic acid, caffeic acid and chlorogenic acid.
As a preferred technical scheme, the peroxide is selected from one or more of sodium peroxide, potassium peroxide, calcium peroxide, barium peroxide, magnesium peroxide and zinc peroxide.
As a preferred technical solution, the bait comprises sugar; the sugar is selected from one or more of glucose, mannose, fructose, galactose, arabinose, xylose, starch, gum arabic, xanthan gum, dextran, mannan, galactan and polyfructose.
As a preferred embodiment, the bait comprises oil; the oil is selected from one or more of adeps medulla bovis Seu Bubali, adeps Sus Domestica, adeps Caprae Seu Ovis, duck oil, goose oil, oleum Rapae, oleum Arachidis Hypogaeae, oleum Sesami, oleum Olivarum, oleum Cocois, oleum Maydis, soybean oil, tea seed oil, palm oil, oleum Helianthi, oleum Juglandis, and oleum ricini.
As a preferred embodiment, the bait comprises a high protein food; the high protein food is selected from one or more of livestock meat, fowl meat, egg, fish, shrimp, beans, and nuts.
As a preferable technical scheme, the preparation raw materials also comprise a medicinal component accounting for 0-10 wt% of the total weight of the bait; the pharmaceutical ingredient is a laxative and/or a cholinergic receptor blocker; the antidiarrheal is selected from one or more of loperamide hydrochloride, diphenoxylate and compound camphor tincture; the choline receptor blocker is selected from one or more of atropine, scopolamine, anisodamine, Prusisin, belladonna tincture, and mecamylamine.
As a preferable technical scheme, the preparation raw material also comprises a water-absorbing substance, and the using amount of the water-absorbing substance accounts for 0-80 wt% of the total amount of the bait.
The second aspect of the invention provides a method for preparing the nontoxic bait which is produced by using the coating technology and affects the digestive system of the rats, and the nontoxic bait is obtained by respectively carrying out capsule coating treatment on the organic acid and the peroxide and then mixing the raw materials.
Has the advantages that: the invention provides a nontoxic bait which is produced by using a coating technology and influences a murine digestive system, which is prepared by adopting a nontoxic raw material and wrapping the raw material which is easy to cause food refusal and eating shortage by a capsule, wherein after the bait is eaten by a mouse, the digestive function of the mouse is damaged, and the intestinal tract is blocked and blocked after being eaten for many times, so that the mouse dies.
Detailed Description
The invention will be further understood by reference to the following detailed description of preferred embodiments of the invention and the examples included therein. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. To the extent that a definition of a particular term disclosed in the prior art is inconsistent with any definitions provided herein, the definition of the term provided herein controls.
As used herein, a feature that does not define a singular or plural form is also intended to include a plural form of the feature unless the context clearly indicates otherwise. It will be further understood that the term "prepared from …," as used herein, is synonymous with "comprising," including, "comprising," "having," "including," and/or "containing," when used in this specification means that the recited composition, step, method, article, or device is present, but does not preclude the presence or addition of one or more other compositions, steps, methods, articles, or devices. Furthermore, the use of "preferred," "preferably," "more preferred," etc., when describing embodiments of the present application, is meant to refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. In addition, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.
In order to solve the above problems, a first aspect of the present invention provides a nontoxic bait for affecting the digestive system of rodents produced using a coating technique, which is prepared from raw materials including at least an organic acid, a peroxide, and a bait; the organic acid and the peroxide are respectively coated by capsules.
The capsule coating treatment in the present application refers to a treatment method for embedding and storing a solid, a liquid or a gas, and specifically may adopt a spray drying method, a spray congealing method, an air suspension method, a vacuum evaporation deposition method, a complex coacervation method, a multi-cavity centrifugation method, a water phase separation method, an oil phase separation method, a capsule core exchange method, an extrusion method, an interface polymerization method, an in situ polymerization method, and the like.
Since both the organic acid and the peroxide are water-soluble compounds, the encapsulation is preferably carried out by in-situ polymerization, oil phase separation, interfacial polymerization, spray drying, or spray congealing.
Taking an in-situ polymerization method for preparing the organic acid capsule as an example, the preparation steps comprise mixing the organic acid and an initiator to be used as a capsule core, dispersing the capsule core in an oily solvent, dripping a capsule shell resin monomer under the condition of heating and stirring, filtering, washing and drying after the reaction is finished, thus obtaining the organic acid capsule. In the above method, the oily solvent may be vegetable oil, and may be rapeseed oil, peanut oil, sesame oil, olive oil, coconut oil, corn oil, soybean oil, tea seed oil, palm oil, sunflower seed oil, walnut oil, castor oil, etc., and the capsule shell resin monomer is preferably a mixture of methacrylic acid and methyl methacrylate, and a surfactant may be appropriately added in order to uniformly disperse the reaction system.
Taking the spray congealing method for preparing peroxide capsules as an example, the preparation steps comprise dispersing peroxide capsule cores in liquefied capsule shell resin, atomizing, and solidifying the capsule shells at low temperature to form capsules. In the above method, the liquefied capsule shell resin may be a resin dissolved in an oily solvent or a resin melted by heating, and in order to reduce the use of the solvent, a resin melted by heating is preferable, wherein the resin may be a Eudragit series product manufactured by romu, germany, and further preferably, Eudragit S100; low temperature means not higher than 30 ℃.
The capsule in this application keeps apart organic acid and peroxide, has guaranteed bait stability and security at the storage transportation, and the smell of active ingredient can still be covered to the capsule during use, and the capsule shell is stable under acidic condition, can slowly release capsule core material under alkaline condition, can keep intact when the capsule passes through mouse stomach promptly, dissolves after getting into the intestinal gradually again, and active ingredient produces the effect in the intestinal, delays the mouse and dies, avoids arousing the mouse alert.
In some preferred embodiments, the preparation raw materials at least comprise 1-50 parts of organic acid, 1-50 parts of peroxide and 100-500 parts of bait by weight.
In some preferred embodiments, the preparation raw materials at least comprise 10-30 parts of organic acid, 5-15 parts of peroxide and 100-500 parts of bait by weight.
In some preferred embodiments, the organic acid is selected from a mixture of one or more of citric acid, malic acid, oxalic acid, succinic acid, tartaric acid, ascorbic acid, quinic acid, shikimic acid, benzoic acid, salicylic acid, caffeic acid, chlorogenic acid; further preferably, the organic acid is selected from one or more of citric acid, malic acid, succinic acid and oxalic acid; further, the organic acid is selected from one or more of citric acid, malic acid and oxalic acid.
In some preferred embodiments, the peroxide is selected from a mixture of one or more of sodium peroxide, potassium peroxide, calcium peroxide, barium peroxide, magnesium peroxide, and zinc peroxide; further preferably, the peroxide is selected from one or more of sodium peroxide, potassium peroxide and calcium peroxide.
In the application, the weight parts of the organic acid and the peroxide are calculated according to the using amount of the effective components before the capsule coating treatment.
In some preferred embodiments, the bait comprises sugar, oil, high protein food.
In some preferred embodiments, the sugar is selected from the group consisting of glucose, mannose, fructose, galactose, arabinose, xylose, starch, gum arabic, xanthan gum, dextran, mannan, galactan, polyfructose, and mixtures thereof.
Examples of the sugar include white sugar, soft sugar, brown sugar, crystal sugar, brown sugar, yellow sugar, and powdered sugar.
Oil in this application refers to edible oil.
In some preferred embodiments, the oil is selected from the group consisting of beef tallow, lard, mutton fat, duck oil, goose oil, rapeseed oil, peanut oil, sesame oil, olive oil, coconut oil, corn oil, soybean oil, tea seed oil, palm oil, sunflower seed oil, walnut oil, castor oil, and mixtures thereof; further preferably, the oil is selected from one or more of beef tallow, lard, mutton fat, sesame oil, soybean oil and corn oil; further, the oil is selected from lard and/or sesame oil.
In some preferred embodiments, the high protein food is selected from a mixture of one or more of livestock meat, poultry meat, eggs, fish, shrimp, legumes, nuts; further preferably, the high-protein food is selected from one or more of chicken, duck, beef, pork, mutton, egg, duck egg, goose egg, fish meal, shrimp meal, soybean, mung bean, red bean, black bean, soybean, peanut, hazelnut, walnut and almond; further, the high protein food is selected from one or more of chicken, egg, fish meal and peanut.
In some preferred embodiments, the weight ratio of sugar, oil, and high protein food is (4-12): (5-15): 1; further preferably, the weight ratio of sugar, oil and high-protein food is 6: 10: 1.
besides the function of attracting mice to eat, the bait has the characteristics of high sugar, high fat and high protein as ingredients, and can cause the constipation of the mice, the pH environment in the intestinal tract is destroyed after the organic acid in the bait is released, the peroxide reacts with the water in the intestinal tract to generate oxygen, the original anaerobic environment is changed, the anaerobic flora in the intestinal tract is disordered, the digestion function is disordered, the intestinal obstruction is caused, the intestinal necrosis, the peritonitis and the like are further developed, and the death is finally realized. The use amounts of the peroxide and the organic acid are controlled in the production process, so that the condition that the rats ingesting the bait are extremely uncomfortable due to excessive use amounts is avoided, other rats in the population are wary, and in addition, if the use amount of the peroxide is excessive relative to the use amount of the organic acid, a large amount of alkali is generated to neutralize the organic acid, so that the effect of the organic acid is weakened.
The inventor finds that the proportion of sugar, oil and protein in the bait can adjust the food intake and death rate of the mice, and the reason is that the sugar can directly adjust the blood sugar content in the animal body, when the sugar is too much, the mice are not easy to have hunger feeling after eating once, and the food can not be rapidly accumulated in the intestinal tract; when the oil is excessive, the oil is insufficiently absorbed by the intestinal tract and is discharged along with excrement, the oil has a lubricating effect, the intestinal obstruction is difficult to induce, and the death rate is reduced; when the protein is excessive, it is not as strong as the oil flavor, and the attraction to mice is slightly low, and the production cost is also increased.
In some preferred embodiments, the raw materials for preparation further comprise 0-10 wt% of a pharmaceutical ingredient based on the total weight of the bait; the pharmaceutical ingredient is a laxative and/or a cholinergic receptor blocker; the antidiarrheal is selected from one or more of loperamide hydrochloride, diphenoxylate and compound camphor tincture; the choline receptor blocker is selected from one or more of atropine, scopolamine, anisodamine, Prusisin, belladonna tincture, and mecamylamine.
The antidiarrheal or cholinergic receptor blocker can act on the smooth muscle of the intestine, the ganglion, the skeletal muscle nerve and other positions, inhibit nerve receptors in the intestinal tract, weaken the intestinal peristalsis, prolong the contact between the intestinal tract contents and the intestinal mucosa, promote the reabsorption of the water in the intestine, harden the excrement and further promote the intestinal blockage of the mice.
In some preferred embodiments, the raw material for preparation further comprises a water-absorbing material, and the water-absorbing material accounts for 0-80 wt% of the total weight of the bait.
In some preferred embodiments, the water absorbing material is selected from one or more of a mixture of acrylic resin, starch, magnesium aluminum silicate, xanthan gum, gum arabic, and powdered silica gel.
The water-absorbing substance can absorb water with the weight of hundreds to thousands of times than the weight of the water-absorbing substance due to the special structure, the water-retaining property is excellent, once the water-absorbing substance absorbs water and expands into hydrogel, the water is difficult to separate out, after the mouse eats the bait, the water-absorbing substance enters the mouse body along with the water-absorbing substance, the activity of hydrophilic groups is limited under an acid environment, the expansion times are limited, but the acting force between the acid groups after entering the intestinal tract is weakened, molecular chains are unfolded, the expansion times are increased, food residues lose water and become dry and hard gradually, and the mouse finally dies due to intestinal obstruction.
The second aspect of the invention provides a method for preparing the nontoxic bait which is produced by using the coating technology and affects the digestive system of the rats, and the nontoxic bait is obtained by respectively carrying out capsule coating treatment on the organic acid and the peroxide and then mixing the raw materials.
Examples
The technical solution of the present invention is described in detail by the following examples, but the scope of the present invention is not limited to the examples. Unless otherwise specified, the starting materials in the present invention are all commercially available.
Example 1
Example 1 provides a nontoxic bait for affecting the digestive system of rats produced by coating technology, which is prepared from 20 parts by weight of organic acid, 10 parts by weight of peroxide and 300 parts by weight of bait.
The organic acid is citric acid; the peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8.
the citric acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of citric acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the modified chitosan/.
The sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
The present invention also provides a method for preparing the nontoxic bait which is produced by the coating technology and affects the digestive system of the rats, wherein the nontoxic bait is obtained by respectively coating organic acid and peroxide in capsules and mixing the raw materials.
And (3) mouse killing test: the rats were fed regularly and quantitatively in this example, 30 rats per group, and the death time and mortality were recorded, and the results are shown in table 1.
TABLE 1
Figure BDA0002389911720000061
Figure BDA0002389911720000071
Example 2
Example 2 provides a nontoxic bait for affecting the digestive system of rats produced by coating technology, which is prepared from 20 parts by weight of organic acid, 10 parts by weight of peroxide, 0.5 part by weight of antidiarrheal agent, and 300 parts by weight of bait.
The organic acid is citric acid; the peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8; the antidiarrheal agent is diphenoxylate (CAS number: 915-30-0).
The citric acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of citric acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the modified chitosan/.
The sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
This example also provides a method of making a nontoxic bait for affecting the digestive system of rodents using coating techniques as described above and similar to example 1.
This example was used for the rat killing test, the test method was the same as example 1, the mortality rate was comparable to example 1, and the death time was slightly shortened.
Example 3
Example 3 provides a nontoxic bait for affecting the digestive system of rats produced by coating technology, which is prepared from 20 parts by weight of organic acid, 10 parts by weight of peroxide, 0.5 part by weight of antidiarrheal agent, 3 parts by weight of water-absorbent resin, and 300 parts by weight of bait.
The organic acid is citric acid; the peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8; the antidiarrheal is diphenoxylate (CAS number: 915-30-0); the water-absorbing resin is acrylic resin powder and is purchased from the hundreds of millions of Pont environmental protection technology Co.
The citric acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of citric acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the modified chitosan/.
The sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
This example also provides a method of making a nontoxic bait for affecting the digestive system of rodents using coating techniques as described above and similar to example 1.
This example was used for the rat killing test, the test method was the same as example 1, the mortality rate was comparable to example 1, and the death time was slightly shortened.
Example 4
Example 4 provides a nontoxic bait for affecting the digestive system of rats produced by coating technology, which is prepared from 20 parts by weight of organic acid, 10 parts by weight of peroxide, 3 parts by weight of water-absorbent resin and 300 parts by weight of bait.
The organic acid is citric acid; the peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8; the water-absorbing resin is acrylic resin powder and is purchased from the hundreds of millions of Pont environmental protection technology Co.
The citric acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of citric acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the modified chitosan/.
The sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
This example also provides a method of making a nontoxic bait for affecting the digestive system of rodents using coating techniques as described above and similar to example 1.
This example was used for the rat killing test, the test method was the same as example 1, the mortality rate was comparable to example 1, and the death time was slightly shortened.
Example 5
Example 5 provides a nontoxic bait for affecting the digestive system of rats produced by coating technology, which is prepared from 20 parts by weight of organic acid, 10 parts by weight of peroxide, 60 parts by weight of xanthan gum and 300 parts by weight of bait.
The organic acid is citric acid; the peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8.
the citric acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of citric acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the modified chitosan/.
The sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
This example also provides a method of making a nontoxic bait for affecting the digestive system of rodents using coating techniques as described above and similar to example 1.
This example was used for the rat killing test, the test method was the same as example 1, the mortality rate was comparable to example 1, and the death time was slightly shortened.
Example 6
Example 6 provides a nontoxic bait for affecting the digestive system of rats produced by coating technology, which is prepared from raw materials including, by weight, 10 parts of organic acid, 5 parts of peroxide, and 300 parts of bait.
The organic acid is oxalic acid; the peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8.
the oxalic acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of oxalic acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the microcapsule.
The sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
This example also provides a method of making a nontoxic bait for affecting the digestive system of rodents using coating techniques as described above and similar to example 1.
This example was used for the rat killing test, the test method was the same as example 1, the mortality rate was comparable to example 1, and the death time was slightly shortened.
Comparative example 1
Comparative example 1 provides a nontoxic bait for affecting the digestive system of rats produced using a coating technique, which is prepared from 20 parts by weight of an organic acid and 300 parts by weight of a bait.
The organic acid is citric acid; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8.
the citric acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of citric acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the modified chitosan/.
The embodiment also provides a preparation method of the nontoxic bait which is produced by using the coating technology and affects the digestive system of the rats, and the nontoxic bait is obtained by coating organic acid in capsules and mixing the organic acid with bait.
This example was used in a rat eradication test, which was the same as example 1, with 3 groups of test rats having a mortality time of greater than 7 days and a mortality rate of less than 60%.
Comparative example 2
Comparative example 2 provides a nontoxic bait for affecting the digestive system of rats produced using a coating technique, which was prepared by using 10 parts by weight of peroxide and 300 parts by weight of bait.
The peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8.
the sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
The embodiment also provides a preparation method of the nontoxic bait which is produced by using the coating technology and affects the digestive system of the rats, and the nontoxic bait is obtained by coating the peroxide in capsules and mixing the peroxide with bait.
This example was used in a rat eradication test, which was the same as example 1, with 3 groups of test rats having a mortality time of greater than 7 days and a mortality rate of less than 60%.
Comparative example 3
Comparative example 3 provides a nontoxic bait for affecting the digestive system of rats produced using a coating technique, which is prepared from 20 parts by weight of an organic acid, 30 parts by weight of a peroxide, and 300 parts by weight of a bait.
The organic acid is citric acid; the peroxide is sodium peroxide; the bait comprises white granulated sugar, lard, fish meal and peanuts, and the weight ratio of the white granulated sugar to the lard to the fish meal is 6: 10: 0.2: 0.8.
the citric acid is processed by capsule coating, and the method comprises the following steps: uniformly mixing 5g of citric acid, 0.2g of ammonium persulfate and 0.6g of sodium stearate, adding the mixture into 110g of rapeseed oil, heating the mixture to 65 ℃, adding 40g of capsule shell resin monomer (the mixture of methacrylic acid and methyl methacrylate with the molar ratio of 1: 2) under the stirring condition, continuously stirring the mixture for 8 hours at the constant temperature of 65 ℃, standing and cooling the mixture, filtering the mixture, washing the mixture with water and drying the mixture to obtain the modified chitosan/.
The sodium peroxide is processed by capsule coating, and the method comprises the following steps: 5g of sodium peroxide was dispersed in 40g of molten Eudragit S100, atomized by an atomizer and solidified at 30 ℃ to form capsules.
This example also provides a method of making a nontoxic bait for affecting the digestive system of rodents using coating techniques as described above and similar to example 1.
This example was used in a rat eradication test, which was the same as example 1, with 3 groups of test rats having a mortality time of greater than 7 days and a mortality rate of less than 60%.
Example 7
Embodiment 7 provides a bait, comprising white granulated sugar, lard, fish meal, peanuts, in a weight ratio of 6: 10: 0.2: 0.8.
comparative example 4
Comparative example 4 provides a bait comprising white granulated sugar, lard, fish meal, peanuts, in a weight ratio of 15: 10: 0.2: 0.8.
comparative example 5
Comparative example 5 provides a bait comprising white granulated sugar, lard, fish meal, peanuts, in a weight ratio of 6: 20: 0.2: 0.8.
example 1, example 6, comparative example 4, comparative example 5 were used for feeding from 140g to 160g of test rats, the observation time was 24h, 15g was dosed, 5 rats were tested per example, the food intake and the defecation were recorded, and the average values were recorded in table 2.
TABLE 2
Examples Food intake Defecation quantity
Example 1 8.0g/24h 5.1g/24h
Example 7 8.2g/24h 7.3g/24h
Comparative example 4 6.6g/24h 6.2g/24h
Comparative example 5 8.5g/24h 8.1g/24h
It can be understood from embodiments 1-6 that the nontoxic bait which is produced by using the coating technology and influences the rodent digestive system can effectively kill rats, and the proper bait can be selected according to different rat suffering degrees. According to the comparison between the embodiment 1 and the embodiment 7, after the organic acid and the peroxide which are coated by the capsule are added into the bait, the suspicion of the rats is not caused, the digestive function of the rats is destroyed after normal feeding, the defecation quantity is reduced, intestinal obstruction is caused by accumulation of hardened excrement in intestinal tracts after a plurality of days, the rats die, the rats do not have the phenomena of acute poisoning and the like in the death process, other rats in the population are not affected, and the overall death rate is improved. In addition, the bait in the application adopts low-toxicity or non-toxic raw materials, has higher safety to animals except mice, kills the mice by acting on the digestive system of the mice, does not cause the mice to generate drug resistance, and can be popularized and used for a long time.
The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims (10)

1. A nontoxic bait produced by coating technology and influencing the digestive system of rats is characterized in that the preparation raw materials at least comprise organic acid, peroxide and bait; the organic acid and the peroxide are respectively coated by capsules.
2. The nontoxic bait for affecting the digestive system of rodents produced by using the coating technology according to claim 1, wherein the raw materials at least comprise 1 to 50 parts by weight of organic acid, 1 to 50 parts by weight of peroxide and 100 to 500 parts by weight of bait.
3. The non-toxic bait for affecting the murine digestive system produced using coating technology according to claim 1 wherein the organic acid is selected from the group consisting of citric acid, malic acid, oxalic acid, succinic acid, tartaric acid, ascorbic acid, quinic acid, shikimic acid, benzoic acid, salicylic acid, caffeic acid, chlorogenic acid in a mixture of one or more thereof.
4. The non-toxic bait for affecting the digestive system of rodents produced using coating technology according to claim 1, wherein said peroxide is selected from the group consisting of sodium peroxide, potassium peroxide, calcium peroxide, barium peroxide, magnesium peroxide and zinc peroxide in combination.
5. A non-toxic bait for affecting the digestive system of rodents produced using coating techniques according to claim 1, wherein said bait comprises sugar; the sugar is selected from one or more of glucose, mannose, fructose, galactose, arabinose, xylose, starch, gum arabic, xanthan gum, dextran, mannan, galactan and polyfructose.
6. The non-toxic bait for affecting the digestive system of rodents produced using coating technology according to claim 1, wherein said bait comprises oil; the oil is selected from one or more of adeps medulla bovis Seu Bubali, adeps Sus Domestica, adeps Caprae Seu Ovis, duck oil, goose oil, oleum Rapae, oleum Arachidis Hypogaeae, oleum Sesami, oleum Olivarum, oleum Cocois, oleum Maydis, soybean oil, tea seed oil, palm oil, oleum Helianthi, oleum Juglandis, and oleum ricini.
7. A non-toxic bait for affecting the digestive system of rodents produced using coating techniques according to claim 1, wherein said bait comprises a high protein food; the high protein food is selected from one or more of livestock meat, fowl meat, egg, fish, shrimp, beans, and nuts.
8. The nontoxic bait for affecting the digestive system of rodents produced according to any one of claims 1 to 7, wherein said raw materials further comprise a pharmaceutical ingredient in an amount of 0 to 10% by weight based on the total weight of the bait; the pharmaceutical ingredient is a laxative and/or a cholinergic receptor blocker; the antidiarrheal is selected from one or more of loperamide hydrochloride, diphenoxylate and compound camphor tincture; the choline receptor blocker is selected from one or more of atropine, scopolamine, anisodamine, Prusisin, belladonna tincture, and mecamylamine.
9. The nontoxic bait for affecting the digestive system of rodents according to any one of claims 1 to 7, wherein said raw material further comprises a water-absorbing material in an amount of 0 to 80% by weight based on the total weight of the bait.
10. A method for preparing the nontoxic bait affecting the digestive system of rats produced by the coating technique according to any one of claims 1 to 10, which is characterized in that the nontoxic bait is prepared by respectively coating organic acid and peroxide in capsules and then mixing the raw materials.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1799365A (en) * 2004-12-31 2006-07-12 潘广英 Innoxious raticide and preparation method thereof
CN101326920A (en) * 2008-07-22 2008-12-24 惠州市南天生物科技有限公司 Raticide
CN102187883A (en) * 2010-12-08 2011-09-21 单承仪 Nontoxic type high expansion solidification lethal rodent bait and preparation method thereof
CN104430324A (en) * 2014-12-04 2015-03-25 兰州大学 Medicament capable of preventing and treating damages caused by rat and preparation method of medicament
EP3384769A1 (en) * 2017-04-05 2018-10-10 Stephen C. Perry Rodenticide

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GB2442202A (en) * 2006-09-30 2008-04-02 Morvus Technology Ltd Vermin poison
CN107921073B (en) * 2015-06-01 2022-10-18 塞诺生物科学股份有限公司 Methods and compositions for regulating gut microbiota and managing weight

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1799365A (en) * 2004-12-31 2006-07-12 潘广英 Innoxious raticide and preparation method thereof
CN101326920A (en) * 2008-07-22 2008-12-24 惠州市南天生物科技有限公司 Raticide
CN102187883A (en) * 2010-12-08 2011-09-21 单承仪 Nontoxic type high expansion solidification lethal rodent bait and preparation method thereof
CN104430324A (en) * 2014-12-04 2015-03-25 兰州大学 Medicament capable of preventing and treating damages caused by rat and preparation method of medicament
EP3384769A1 (en) * 2017-04-05 2018-10-10 Stephen C. Perry Rodenticide

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