CN111196632B - Rhodotorula mucilaginosa for removing total nitrogen in sewage and application thereof - Google Patents
Rhodotorula mucilaginosa for removing total nitrogen in sewage and application thereof Download PDFInfo
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- CN111196632B CN111196632B CN202010039266.2A CN202010039266A CN111196632B CN 111196632 B CN111196632 B CN 111196632B CN 202010039266 A CN202010039266 A CN 202010039266A CN 111196632 B CN111196632 B CN 111196632B
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 148
- 239000010865 sewage Substances 0.000 title claims abstract description 77
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 72
- 241000223254 Rhodotorula mucilaginosa Species 0.000 title claims abstract description 57
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims abstract description 44
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims abstract description 15
- 239000007789 gas Substances 0.000 claims abstract description 13
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 239000002440 industrial waste Substances 0.000 claims abstract description 8
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 4
- 230000015556 catabolic process Effects 0.000 claims description 9
- 238000006731 degradation reaction Methods 0.000 claims description 9
- 239000002351 wastewater Substances 0.000 claims description 7
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- 230000000593 degrading effect Effects 0.000 abstract description 7
- 230000012010 growth Effects 0.000 abstract description 3
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 abstract description 2
- 239000013535 sea water Substances 0.000 abstract description 2
- 239000010802 sludge Substances 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 241000222178 Candida tropicalis Species 0.000 description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 239000007788 liquid Substances 0.000 description 8
- 230000002035 prolonged effect Effects 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 6
- 238000012258 culturing Methods 0.000 description 5
- 239000001963 growth medium Substances 0.000 description 5
- 238000009630 liquid culture Methods 0.000 description 5
- 230000014759 maintenance of location Effects 0.000 description 5
- 125000001477 organic nitrogen group Chemical group 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 238000005273 aeration Methods 0.000 description 4
- 239000000945 filler Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 210000005253 yeast cell Anatomy 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000003307 slaughter Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 241000223252 Rhodotorula Species 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 239000000306 component Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000012851 eutrophication Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 230000001195 anabolic effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000003501 co-culture Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
- 239000010840 domestic wastewater Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
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- 229920002635 polyurethane Polymers 0.000 description 1
- 239000004814 polyurethane Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- 239000002912 waste gas Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2257/00—Components to be removed
- B01D2257/40—Nitrogen compounds
- B01D2257/406—Ammonia
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/08—Chemical Oxygen Demand [COD]; Biological Oxygen Demand [BOD]
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Abstract
The invention discloses a rhodotorula mucilaginosa for removing total nitrogen in sewage and application thereof, which is characterized in that the rhodotorula mucilaginosa (or the rhodotorula mucilaginosa) (A)Rhodotorula mucilaginosa) NBUY-9, deposited in the China center for type culture Collection in 2019, 12 months and 5 days, with the deposit number: CCTCC NO: m20191015, the rhodotorula mucilaginosa grows on a YPD plate without seawater salinity, the colony morphology is characterized by orange red, round, smooth, moist and opaque surface, the strain can grow in a wide range of pH 2.5-9.0, the optimal growth temperature is 20-30 ℃, and the rhodotorula mucilaginosa can be applied to preparation of a total nitrogen remover, an ammonia nitrogen remover and a COD remover in domestic sewage and preparation of an ammonia gas degrading agent in industrial waste gas.
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and particularly relates to rhodotorula mucilaginosa for simultaneously removing ammonia nitrogen and total nitrogen in sewage and application thereof.
Background
The rapid development of economy leads to large urbanization scale, increasingly aggravated human activities, larger and larger domestic sewage discharge, and the phenomenon of water eutrophication caused by over-high nitrogen concentration in water is a global water pollution problem. The comprehensive discharge standard of urban sewage is newly established in 2002 in China (GB 19818-2002), the discharge standard of urban domestic sewage treatment is increased, and the first-class A standard of the highest allowable discharge concentration (daily mean mg/L) is as follows: ammonia nitrogen (NH 3-N) is less than or equal to 5; total Nitrogen (TN) is less than or equal to 15; CODCrLess than or equal to 50. In recent years, more strict local discharge standards of town sewage plants are established in many provinces and cities in China, such as Zhejiang local standard DB33/2169 plus 2018, and the ammonia nitrogen (NH 3-N) of the existing town sewage plant is required to be less than or equal to 2; total Nitrogen (TN) is less than or equal to 12; newly building a town sewage plant, wherein the ammonia nitrogen (NH 3-N) is less than or equal to 1.5; total Nitrogen (TN) is less than or equal to 10. The enhanced biological denitrification is a difficult point and a key node for the design and operation management of the urban sewage treatment plant. The concentrations of ammonia nitrogen, total nitrogen and COD in the inlet water of a common town sewage plant are respectively 30 mg/L, 40 mg/L and 180 mg/L, and the pH value is 6.8-7.2. Activated sludge of a domestic sewage treatment plant is domesticated and then is used for treating sewage, ammonia nitrogen can reach the standard within relatively short treatment time, total nitrogen needs to flow nitrified liquid back to an anoxic tank for denitrification treatment, the total nitrogen treatment efficiency is low, the sewage treatment time needs to be prolonged, namely, the hydraulic retention time is prolonged, and the total nitrogen can be discharged after reaching the standard, so that the energy consumption and the price of the sewage treatment are increased. At present, the treatment capacity of domestic sewage is increasingly large, so that the problems of low treatment efficiency, increased standard capacity expansion pressure and the like of the existing sewage treatment plant are increasingly prominent, and the problems can not be solved by completely depending on a newly-built sewage treatment plant. Fully excavates the potential of the existing sewage treatment plant, carries out the upgrading and the volume expansion transformation, improves the biological denitrification efficiency, and is the solution for the future in ChinaImportant means of environmental issues.
At present, domestic research on denitrifying microorganisms mainly focuses on the degradation effect of mixed floras and the improvement of a denitrifying denitrification process, pure bacteria with a total nitrogen removal function are not separated, denitrification can be completed only by starting a reactor and acclimatizing and culturing the mixed floras, the number of processes is large, and the energy consumption and the time are long. Aiming at the phenomena, when ammonia nitrogen in sewage is required to be removed, the total nitrogen in the sewage is removed in a relatively short treatment time, the hydraulic retention time of sewage treatment is reduced, and the problem that the total nitrogen is difficult to reach the discharge standard is solved, so that the discharge amount of the total nitrogen is reduced, and the eutrophication phenomenon of a water body is reduced.
Disclosure of Invention
The invention aims to provide rhodotorula mucilaginosa for removing total nitrogen in sewage and application thereof, wherein the rhodotorula mucilaginosa can simultaneously and efficiently remove ammonia nitrogen, total nitrogen and COD in sewage and efficiently degrade ammonia components in industrial waste gas.
The technical scheme adopted by the invention for solving the technical problems is as follows: rhodotorula mucilaginosa for removing total nitrogen in sewage, Rhodotorula mucilaginosa (B)Rhodotorula mucilaginosa) NBUY-9 strain, deposited in the China center for type culture Collection in 2019, 12 months and 5 days, with the deposit number: CCTCC NO: m20191015.
The rhodotorula mucilaginosa grows on a YPD plate without seawater salinity, the morphological characteristics of a colony are orange red, round, smooth, moist and opaque on the surface, the strain can grow in a wider range of pH 2.5-9.0, and the optimal growth temperature is 20-30 ℃.
The rhodotorula mucilaginosa for removing the total nitrogen in the sewage is applied to the preparation of the total nitrogen remover in the sewage.
The rhodotorula mucilaginosa for removing the total nitrogen in the sewage is applied to the preparation of the ammonia nitrogen remover in the sewage.
The rhodotorula mucilaginosa for removing the total nitrogen in the sewage is applied to the preparation of a COD remover in the sewage.
The rhodotorula mucilaginosa for removing the total nitrogen in the sewage is applied to the preparation of ammonia degradation agent in industrial waste gas.
Compared with the prior art, the invention has the advantages that: the Rhodotorula mucilaginosa NBUY-9 strain is obtained by separating and purifying activated sludge in an aerobic pool of a sewage treatment station of a fish processing plant, can efficiently remove ammonia nitrogen, total nitrogen and COD in sewage containing inorganic nitrogen, efficiently remove the Rhodotorula mucilaginosa containing ammonia nitrogen, total nitrogen and COD in domestic sewage containing organic nitrogen, and efficiently degrade industrial waste gas containing ammonia gas, and has wide application range.
The above Rhodotorula mucilaginosa (B) ((B))Rhodotorula mucilaginosa) The strain is NBUY-9 strain, and the preservation number is CCTCC NO: m20191015, deposited in the China center for type culture Collection in 2019, 12 and 5 months, with the deposit address of Wuhan university, Wuhan, China.
Drawings
FIG. 1 is a schematic diagram showing the effect of Rhodotorula mucilaginosa NBUY-9 in degrading industrial waste gas containing ammonia gas.
Detailed Description
The invention is described in further detail below with reference to the accompanying examples.
Detailed description of the preferred embodiment
Isolation source of rhodotorula mucilaginosa NBUY-9 strain
1. Weighing 10 g of activated sludge in an aerobic pool of a sewage treatment station of a certain fish processing plant in Ningbo city, inoculating the activated sludge into a triangular flask filled with a 90 m1 YPD liquid culture medium, placing the triangular flask under the conditions of 30 ℃ and 150 r/min for shake culture, adding 5-15 ml of 10% ammonia water into the culture medium every 24 hours to enrich nitrogen-resistant yeast, after culturing for 4 days, taking the liquid culture medium, adding the liquid culture medium into another fresh YPD liquid culture solution according to the volume ratio of 10%, repeating for 3 times, and carrying out co-culture for 12 days to obtain a yeast culture solution; wherein YPD liquid medium components (g/L): 10.0 parts of yeast powder, 20 parts of peptone and 20 parts of glucose, wherein the pH value is 6.5, and 1L of distilled water is obtained.
2. Diluting the obtained yeast culture solution, spreading on YPD solid medium plate, culturing at 30 deg.C for 3 d, selecting single colony, streaking, separating and purifying, repeating for 2 times to obtain pure bacteria, and numbering as NBUY-9; YPD solid Medium composition (g/L): 10 parts of yeast powder, 20 parts of peptone and 20 parts of glucose, wherein the pH value is 6.5, the agar is 1.5 percent, and 1L of distilled water is obtained.
3. Identification of strains
(1) Morphological characteristics: the colony morphology is characterized by orange red, round, smooth surface, moist and opaque. The cell shape of the strain is circular and elliptical, and the strain is propagated by budding at multiple edges;
(2) molecular biological identification: PCR amplification and DNA sequencing of The yeast 26S rDNA using a 26S rDNA universal primer, alignment of The obtained sequence with The known sequence published in GenBank of The National Center for Biotechnology Information (NCBI) of The United states by Blastn software (http:// blast. NCBI. nlm. nih. gov), comparison of The target strain with The known Rhodotorula mucilaginosa (NCBI. Nih. gov)Rhodotorula mucilaginosa) The similarity of the strain with the accession number of EU285542.1 reaches 100 percent, so the strain is identified as the rhodotorula mucilaginosaRhodotorula mucilaginosa. Wherein the sample 26S rDNA sequence of the present application is:
GCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAGCGGCGAGCGAAGCGGGAAGAGCTCAAATTTATAATCTGGCACCTTCGGTGTCCGAGTTGTAATCTCTAGAAATGTTTTCCGCGTTGGACCGCACACAAGTCTGTTGGAATACAGCGGCATAGTGGTGAGACCCCCGTATATGGTGCGGACGCCCAGCGCTTTGTGATACATTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAATTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAACAGTACGTGAAATTGTTGGAAGGGAAACGCTTGAAGTCAGACTTGCTTGCCGAGCAATCGGTTTGCAGGCCAGCATCAGTTTTCCGGGATGGATAATGGTAGAGAGAAGGTAGCAGTTTCGGCTGTGTTATAGCTCTCTGCTGGATACATCTTGGGGGACTGAGGAACGCAGTGTGCCTTTGGCGGGGGTTTCGACCTCTTCACACTTAGGATGCTGGTGGAATGGCTTTAAACGACCCGTCTTGACACACGGACCA；
(3) the strain is named as rhodotorula mucilaginosa (by combining molecular biological identification and morphological characteristics)Rhodotorula mucilaginosa) NBUY-9 with the preservation number of CCTCC NO: m20191015, deposited in the China center for type culture Collection in 2019, 12/5, with the deposit address of Wuhan, Wuhan university, hereinafter referred to as Rhodotorula mucilaginosa NBUY-9.
Detailed description of the invention
Culturing of Rhodotorula mucilaginosa NBUY-9 cells: taking 2 environment-friendly collection numbers as CCTCC NO: m2019101Rhodotorula mucilaginosa of (5)Rhodotorula mucilaginosa) NBUY-9 was inoculated into 100 mL YPD liquid medium, cultured with shaking at 28 ℃ and 180 r/min for 24 hours to obtain yeast cell sap, and 12 g of wet cells were used as an experimental group for the following sewage treatment;
another strain of yeast preserved in the laboratory, candida tropicalis ((R))Candida tropicalis) ZJY-7 served as experimental control group 1. Culturing cells by the same method, and collecting 2-ring Candida tropicalis (C.tropicalis: (C.tropicalis)Candida tropicalis) ZJY-7 strain was inoculated into 100 mL YPD liquid medium, cultured with shaking at 28 ℃ and 180 r/min for 24 h to obtain yeast cell liquid, and 12 g of wet cells were used as control group 1 in the following sewage treatment.
A certain amount of activated sludge of a certain municipal domestic sewage treatment plant is taken, domesticated for a certain time, and then 12 g of sludge is weighed as a control group 2 and used in the following sewage treatment. The Total Nitrogen (TN) is divided into inorganic nitrogen and organic nitrogen, the inorganic nitrogen mainly comprises ammonia nitrogen, nitrate nitrogen and nitrite nitrogen, and the organic nitrogen mainly comprises protein, amino acid, polypeptide, urea and other nitrogen-containing organic matters.
Example 1
Application of Rhodotorula mucilaginosa NBUY-9 in degradation of ammonia nitrogen, total nitrogen and COD in artificial sewage containing inorganic nitrogen
Artificial sewage: preparing 3L sewage by using glucose and ammonium chloride according to the ratio of C: N =100:5, and adding a small amount of K2HPO4The pH value is adjusted to be 6.5-8.0, and the concentration of ammonia nitrogen, TN and COD in the prepared artificial sewage is respectively about 40 mg/L, 40 mg/L and 200 mg/L. The experimental group sample, the control group 1 sample and the control group 2 sample were added to the same circular reactor, and 1L of artificial sewage was added to 3 reactors, respectively. The reactor is provided with an aeration device, the dissolved oxygen is about 4mg/L, and the top of the reactor is sealed by a sterile sealing film to prevent the entry of mixed bacteria. Then aeration treatment is carried out. Sampling every 1 h, and determining that the pH value is about 6.5-8.0 to reach the range of the emission standard; and detecting the concentration changes of ammonia nitrogen, total nitrogen and COD in the sample, wherein the data are the mean values of parallel sample determination. The experiment was run for 8 h and the data is as follows:
TABLE 1 Effect of Rhodotorula mucilaginosa NBUY-9 on degrading inorganic nitrogen-containing wastewater in experimental group
TABLE 2 Effect of Candida tropicalis degrading inorganic Nitrogen-containing Sewage in control group 1
TABLE 3 Effect of the activated sludge of control group 2 on degrading inorganic nitrogen-containing wastewater
Remarking: the total nitrogen in tables 1-3 refers to inorganic nitrogen.
As can be seen from the results in tables 1-3, the ammonia nitrogen in Rhodotorula mucilaginosa NBUY-9 experimental group reaches the standard after 4 hours of treatment, and the removal rate is 90.0%; the total nitrogen reaches the standard within 6 h, and the removal rate is 65.0 percent; COD reached the standard in 7 h, and the removal rate is 76.1 percent. The ammonia nitrogen of the candida tropicalis control group 1 reaches the standard within 8 h, the removal rate is 89.5%, the total nitrogen is not degraded basically, and the removal rate within 8 h is only 3.7%, so that the COD is not measured. The ammonia nitrogen of the activated sludge control group 2 reaches the standard within 8 h, the removal rate is 87.7 percent, the total nitrogen degradation is small, the ammonia nitrogen does not reach the standard within 8 h, and the removal rate is 20 percent; COD reached the standard in 5 h, and the removal rate is 79.1 percent. Experimental results prove that the rhodotorula mucilaginosa NBUY-9 has the functions of removing ammonia nitrogen and total nitrogen in sewage, the total nitrogen removed after the ammonia nitrogen in the sewage is removed for 4 hours reaches the standard, the total nitrogen removed after the treatment is continued for 2 hours is prolonged, and the COD reaches the standard after 3 hours. While other yeasts used as a control can utilize a nitrogen source in water in the growth anabolic process, but only have the function of removing ammonia nitrogen in sewage, and do not have the function of removing total nitrogen. The activated sludge of a certain municipal domestic sewage treatment plant as a contrast only has the function of removing sewage ammonia nitrogen, has smaller function of removing total nitrogen, has the ammonia nitrogen removal function which is poorer than that of the rhodotorula mucilaginosa NBUY-9, the ammonia nitrogen can reach the standard after being prolonged for 4 hours, the total nitrogen is degraded by 20 percent, and the hydraulic retention time is prolonged until the standard value of the total nitrogen removal rate of 62.5 percent reaches 15 mg/L, and the total nitrogen can reach the standard and be discharged after being treated for about 24 hours.
Example 2
The Rhodotorula mucilaginosa NBUY-9 is used for degrading ammonia nitrogen, total nitrogen and COD in domestic sewage containing organic nitrogen.
The domestic sewage of a certain sewage treatment plant is taken as an experimental object, and the concentration of ammonia nitrogen, total nitrogen and COD is respectively measured to be 30 mg/L, 40 mg/L and 183.7 mg/L. The experimental group sample, the control group 1 sample and the control group 2 sample were added to the same circular reactor, and 1L of domestic sewage was added to 3 reactors, respectively. The reactor is provided with an aeration device, the dissolved oxygen is about 4mg/L, the top of the reactor is sealed by a sterile sealing film to prevent the entry of mixed bacteria, and then aeration treatment is carried out. Sampling every 1 hour, and determining that the pH value is about 6.5-8.0 to reach the range of the emission standard; and the concentration changes of ammonia nitrogen, total nitrogen and COD in the sample are detected, the experiment is carried out for 8 hours, and the data is as follows:
TABLE 4 Effect of the experimental group Rhodotorula mucilaginosa NBUY-9 on degradation of domestic wastewater
TABLE 5 Effect of Candida tropicalis degradation on domestic Sewage in control group 1
TABLE 6 Effect of degrading domestic Sewage by activated sludge of control group 2
Remarking: the total nitrogen in tables 4 to 6 means that organic nitrogen is contained.
The results in tables 4-6 show that the Rhodotorula mucilaginosa NBUY-9 experimental group reaches the standard after treating ammonia nitrogen for 4 hours, and the removal rate is 85.7%; the total nitrogen reaches the standard within 5 h, and the removal rate is 71.5 percent; COD reached the standard in 6 h, and the removal rate is 73.0 percent. The ammonia nitrogen of the candida tropicalis control group 1 reaches the standard within 6 h, the removal rate is 84.7%, the total nitrogen is not degraded basically, and the removal rate is only 3.5% within 8 h, so that the COD is not measured. The ammonia nitrogen of the activated sludge control group 2 reaches the standard approximately within 8 hours, namely 5.1 mg/L, the removal rate is 83 percent, the total nitrogen degradation is small, the ammonia nitrogen does not reach the standard within 8 hours, and the removal rate is 10 percent; COD reached the standard in 4 h, and the removal rate is 73.2 percent. Experimental results prove that the rhodotorula mucilaginosa NBUY-9 has the function of removing ammonia nitrogen and total nitrogen from domestic sewage, the total nitrogen removed after the ammonia nitrogen is removed from the sewage in 4 hours reaches the standard, the total nitrogen removed after the treatment is prolonged for 1 hour reaches the standard, and the COD reaches the standard after 2 hours. And other yeasts used as a reference only have the function of removing ammonia nitrogen in sewage, but do not have the function of removing total nitrogen. The activated sludge of a certain municipal domestic sewage treatment plant as a contrast only has the function of removing ammonia nitrogen from domestic sewage, and has a smaller function of removing total nitrogen, the function of removing ammonia nitrogen is poorer than that of the rhodotorula mucilaginosa NBUY-9, the ammonia nitrogen needs to be prolonged for 5 hours, namely the ammonia nitrogen can reach the standard after being treated for 9 hours, the total nitrogen is degraded by about 10 percent, the hydraulic retention time needs to be prolonged until the standard reaching value of 62.5 percent of the total nitrogen removal rate is 15 mg/L, and the total nitrogen can reach the standard after being treated for about more than 24 hours and then is discharged.
In summary, the Rhodotorula mucilaginosa (B) of the present inventionRhodotorula mucilaginosa) NBUY-9 is applied to the treatment of artificial sewage and domestic sewage, ammonia nitrogen reaches the standard after 4 hours of treatment, total nitrogen reaches the standard after 5-6 hours of treatment, and COD reaches the standard after 6-7 hours. The rhodotorula mucilaginosa NBUY-9 which is screened by the inventor and has the function of removing the total nitrogen in the sewage can simultaneously remove the ammonia nitrogen and the total nitrogen in the sewage in a relatively short treatment time, and the problem that the total nitrogen is difficult to reach the discharge standard is solved.
Detailed description of the preferred embodiment
Rhodotorula mucilaginosa NBUY-9 degradation of industrial waste gas containing ammonia gas component and application thereof
A biotrickling filter tower is built outside a slaughtering workshop of a certain poultry slaughtering factory in Ningbo city, the size of the tower is phi 3000H 7000, the filler is divided into two layers, the upper layer filler is a ceramic pall ring and is 0.6 m high, and the lower layer filler is polyurethane and is 0.6 m high. Collecting waste gas of slaughtering workshopAnd is discharged into a biological trickling filter. Inoculating rhodotorula mucilaginosa NBUY-9 into a YPD liquid culture medium in a laboratory, carrying out shaking culture at 28 ℃ and 180 r/min for 24 h to obtain 50L of expanded culture yeast cell liquid, pouring 50L of yeast cell liquid into a nutrient solution water storage tank matched with the outside of a biological trickling filter, adding 2 tons of effluent discharged from a factory sewage treatment station to a secondary pipe network of an industrial park in the water storage tank as nutrient solution (containing 80-200 mg/L of COD and 30-35 mg/L of ammonia nitrogen), lifting the nutrient solution containing the rhodotorula mucilaginosa NBUY-9 cells to the top of the biological trickling filter through a circulating water pump, and spraying downwards to enable the filler to be coated for 3 d under the circulating spray. After the membrane is formed, the circulating liquid is directly emptied from the tower and flows to a sewage treatment station. Replacing new sewage treatment station in the water storage tank to discharge water as nutrient solution, starting to operate the biological trickling filtration tower, and setting the maximum wind quantity of the fan at 40000 m3And h, the retention time EBRT of the discharged gas in the empty bed is 2 s, the ammonia gas concentration at the gas inlet and the gas outlet is detected 1 time per day, the operation is carried out for 1 month, and the result is shown in figure 1.
As can be seen from FIG. 1, the ammonia gas concentration at the inlet is 1.27-2.11 mg/m during the 1 month startup operation period3The concentration of ammonia gas at the gas outlet is 0.08-0.17 mg/m3The ammonia gas removal rate of the bio-trickling filter applying the rhodotorula mucilaginosa NBUY-9 is 86.7-96%. The tail gas is discharged through a 15 m high chimney, and reaches the emission standard (GB 14554-1993 national standards for the emission of malodorous pollutants).
In summary, the Rhodotorula mucilaginosa (B) of the present inventionRhodotorula mucilaginosa) NBUY-9 can be applied to removing total nitrogen in domestic sewage and can also be applied to a biological trickling filter to treat industrial waste gas containing ammonia gas.
The above description is not intended to limit the present invention, and the present invention is not limited to the above examples. Those skilled in the art should also realize that changes, modifications, additions and substitutions can be made without departing from the true spirit and scope of the invention.
Sequence listing
<110> Ningbo university
<120> Rhodotorula mucilaginosa for removing total nitrogen in sewage and application thereof
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 614
<212> DNA
<213> Artificial sequence
<220>
<223> Rhodotorula mucilaginosa (Rhodotorula mucolignosa)
<400> 1
GCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAGCGGCGAGCGAAGCGGGAAGAGCTCAAATTTATAATCTGGCACCTTCGGTGTCCGAGTTGTAATCTCTAGAAATGTTTTCCGCGTTGGACCGCACACAAGTCTGTTGGAATACAGCGGCATAGTGGTGAGACCCCCGTATATGGTGCGGACGCCCAGCGCTTTGTGATACATTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCAAATTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAACAGTACGTGAAATTGTTGGAAGGGAAACGCTTGAAGTCAGACTTGCTTGCCGAGCAATCGGTTTGCAGGCCAGCATCAGTTTTCCGGGATGGATAATGGTAGAGAGAAGGTAGCAGTTTCGGCTGTGTTATAGCTCTCTGCTGGATACATCTTGGGGGACTGAGGAACGCAGTGTGCCTTTGGCGGGGGTTTCGACCTCTTCACACTTAGGATGCTGGTGGAATGGCTTTAAACGACCCGTCTTGACACACGGACCA 614
Claims (5)
1. A Rhodotorula mucilaginosa for removing total nitrogen in sewage is characterized in that the Rhodotorula mucilaginosa (A) and (B) areRhodotorula mucilaginosa) NBUY-9 strain, deposited in the China center for type culture Collection in 2019, 12 months and 5 days, with the deposit number: CCTCC NO: m20191015.
2. Use of the Rhodotorula mucilaginosa for removing total nitrogen from wastewater according to claim 1 in preparing a total nitrogen removing agent from wastewater.
3. The application of the rhodotorula mucilaginosa for removing total nitrogen in sewage, which is disclosed by claim 1, in the preparation of an ammonia nitrogen remover in sewage.
4. The use of the Rhodotorula mucilaginosa for removing total nitrogen from wastewater according to claim 1 in the preparation of COD remover from wastewater.
5. Use of the Rhodotorula mucilaginosa for removing total nitrogen from wastewater according to claim 1 in the preparation of ammonia degradation agent in industrial waste gas.
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