CN101928067A - Culture method of aerobic denitrification particle sludge - Google Patents

Culture method of aerobic denitrification particle sludge Download PDF

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Publication number
CN101928067A
CN101928067A CN2009100403641A CN200910040364A CN101928067A CN 101928067 A CN101928067 A CN 101928067A CN 2009100403641 A CN2009100403641 A CN 2009100403641A CN 200910040364 A CN200910040364 A CN 200910040364A CN 101928067 A CN101928067 A CN 101928067A
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sludge
reactor
aerobic denitrification
aerobic
waste water
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CN101928067B (en
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徐勇军
谢磊
杨晓西
刘治猛
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Dongguan University of Technology
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Dongguan University of Technology
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Abstract

The invention relates to the technical field of a culture method of particle sludge in a biological treatment technology of waste water, in particular to a culture method of aerobic denitrification particle sludge. The method comprises the following steps: putting inoculated activated sludge into an upflow-type aerobic sludge bed reactor; pumping in waste water containing nitrite and nitrate and organic matters from the bottom of the reactor; ensuring that the waste water flows out of the upper part of the reactor after passing through the reactor; and meanwhile, adopting air aeration, obtaining favourable aerobic denitrification particle sludge after the materials are cultured for 35-120 days. The aerobic denitrification particle sludge obtained by the method has good performance, and the volume loading, the removal efficiency and the operation stability of the reactor can be improved.

Description

A kind of cultural method of aerobic denitrification particle sludge
Technical field:
The present invention relates to the cultural method technical field of the granule sludge in the biological wastewater treatment technology, particularly a kind of cultural method of aerobic denitrification particle sludge.
Background technology:
In recent years, water pollutes serious day by day, and too much nitrogen-containing pollutant enters natural water body, not only makes quality of water environment worsen, and influences the development of fishery, tourism, aquaculture, but also the existence of HUMAN HEALTH and animal and plant is brought serious harm.Each state has all launched the research to wastewater treatment, wherein, utilize biotechnology to handle waste water and more and more be subject to people's attention, and the wastewater biological denitrificaion technology has caused the common concern of countries in the world.
The traditional biological denitride technology comprises aerobic nitrification and two processes of anaerobic denitrifying, has following drawback: aeration tank, investment and working cost height that technical process is long, needs are bigger; If will keep higher biological concentration and good denitrification effect, must carry out mud and nitrification liquid simultaneously and reflux; System's impact resistance a little less than, high density contains NH 4+And NO 2-Waste water can suppress nitrobacteria growth; The acidity that produces in the nitrifying process need drop into alkali and neutralize, and has increased processing costs, also may cause secondary pollution etc.
In recent years, the aerobic denitrification technology has obtained investigator's attention.This technology can realize synchronous nitration, denitrification, and promptly aerobic denitrification can take place in same reactor with nitration reaction, has reduced system space and construction costs, has also reduced operation easier and running cost.
However, the aerobic denitrification biological denitrificaion is owing to adopting the suspension activated sludge process, so still there are lower, the problems such as processing efficiency is lower, fluctuation of service of load at present.
Summary of the invention:
The objective of the invention is to provide a kind of cultural method of aerobic denitrification particle sludge at the deficiencies in the prior art, it can solve low, the problems such as reactor efficiency is low, investment working cost height of sludge concentration that exist in traditional nitrated, denitrification biological denitrogenation technology, effectively reduces simultaneously to contain the pollution that nitrate and nitrite waste water cause water body in the industries such as chemical fertilizer, breed.
For achieving the above object, the cultural method of a kind of aerobic denitrification particle sludge of the present invention adopts following technical scheme:
1, at first chooses one or more upflowing aerobic sludge bed bioreactors of packing into and being furnished with triphase separator in aerobic floc sludge, anaerobism floc sludge, the anaerobic grain sludge;
2, then waste water is pumped into from reactor bottom, water intake mode is intermittent type or continous way; Reactor bottom is equipped with aeration head, adopts air aeration when waste water is flowed through reactor, and air speed is 25~50L/h, utilizes air-flow to shear generation and the growth that promotes granule sludge;
3, keep the interior temperature of reactor between 25~35 ℃, the pH value is between 6~8, and dissolved oxygen is between 3~6mg/L, and hydraulic detention time is 6-10 hour, cultivates after 35~120 days, obtains aerobic denitrification particle sludge in reactor.
Wherein, the substrate in the granule sludge culturing process is for containing nitrate, nitrite and organic waste water, and total nitrate concentration comprises nitrate and nitrite, is 10~500mg/L, and the molar ratio of nitrite and nitrate is 1: 5-5: 1; Organism includes methyl alcohol, ethanol, glucose etc.
In addition, should adopt the method that progressively improves water inlet nitrate and nitrite concentration in the granule sludge culturing process, help obtaining the aerobic denitrification particle sludge of a large amount of enrichments of denitrifying bacteria.
The aerobic denitrification particle sludge outward appearance that obtains according to the method for the invention is the tawny irregular particle, and particle diameter is 0.6~4mm, and density is 1.02~1.40g/cm 3Functional, can improve reactor volumetric loading, remove efficient and operation stability.
Granule sludge has advantages such as microbial biomass height, settleability are good, anti impulsion load, can be used for removing various organism, ammonia nitrogen, phosphorus and absorption heavy metal etc.The present invention turns out the aerobic denitrification particle sludge with high-activity biological denitrogenation ability, improved the denitrification denitrogenation efficient of ammonia nitrogen waste water, simultaneously also can be used for containing the efficient removal of nitrate and nitrite waste water, thereby can effectively reduce the nitrogenous effluent pollution on the environment.
Embodiment:
Embodiment 1
The anaerobic grain sludge in UASB (the Up-flow Anaerobic Sludge Bed up-flow anaerobic sludge blanket) reactor of handling starch wastewater and the aerobic floc sludge of Treating Municipal Sewage are pressed 4: 1 mixed of mass ratio, then mixing sludge is inoculated in the upflowing aerobic sludge bed bioreactor.The total nitrate concentration of human configuration simulated wastewater is 300mg/L, wherein the mol ratio of nitrite and nitrate is 2: 1, reactor operating condition is: 25~35 ℃ of temperature, the pH value is between 6.4~7.8, dissolved oxygen is at 3~6mg/L, and hydraulic detention time is 6~10 hours, adopts air aeration, air speed is 20~30L/h, and the aerobic denitrification particle sludge of brown is observed in steady running in reactor after 72 days.Its particle diameter 0.7~1.1mm, density is 1.01~1.28g/cm 3, total nitric nitrogen clearance is 77%.
Embodiment 2
Adopt the seed sludge identical with embodiment 1, the total nitrate concentration of water inlet is progressively brought up to 300mg/L by 30mg/L in the culturing process, improved an influent load in per 3 days, each 30mg/L that improves, other conditions are identical with embodiment 1, the aerobic denitrification particle sludge of brown is observed in steady running in reactor after 65 days, its particle diameter 0.8~1.2mm, the about 1.15g/cm of density 3, total nitric nitrogen clearance is 83%.
Embodiment 3
Adopt the active sludge in certain slaughterhouse Sewage treatment systems to be inoculated in the upflowing aerobic reactor, the total nitrate concentration of human configuration simulated wastewater is 400mg/L, wherein the mol ratio of nitrite and nitrate is 1: 2, the reactor air speed is 30~45L/h, other conditions are identical with embodiment 1, in the continuous 89 days post-reactors aerobic denitrification particle sludge appears, its particle diameter 2.8~3.5mm, the about 1.25g/cm of density 3, total nitric nitrogen clearance is 85%.

Claims (6)

1. the cultural method of an aerobic denitrification particle sludge, it is characterized in that: it may further comprise the steps:
(1), chooses one or more upflowing aerobic sludge bed bioreactors of packing into and being furnished with triphase separator in aerobic floc sludge, anaerobism floc sludge, the anaerobic grain sludge;
(2), waste water is pumped into from reactor bottom;
(3), keep temperature in the reactor between 25~35 ℃, the pH value is between 6~8, dissolved oxygen is between 3~6mg/L, hydraulic detention time is 6-10 hour, cultivates after 35~120 days, obtains aerobic denitrification particle sludge in reactor.
2. the cultural method of a kind of aerobic denitrification particle sludge according to claim 1, it is characterized in that: wherein, waste water is for containing nitrate, nitrite and organic waste water, total nitrate concentration, comprise nitrate and nitrite, be 10~500mg/L, and the molar ratio of nitrite and nitrate is 1: 5-5: 1.
3. the cultural method of a kind of aerobic denitrification particle sludge according to claim 1 is characterized in that: adopt the method that progressively improves water inlet nitrate and nitrite concentration in the granule sludge culturing process.
4. the cultural method of a kind of aerobic denitrification particle sludge according to claim 1, it is characterized in that: the aerobic denitrification particle sludge outward appearance that obtains is the tawny irregular particle, and particle diameter is 0.6~4mm, and density is 1.02~1.40g/cm 3
5. the cultural method of a kind of aerobic denitrification particle sludge according to claim 1, it is characterized in that: the water intake mode that pumps into waste water is intermittent type or continous way.
6. the cultural method of a kind of aerobic denitrification particle sludge according to claim 1, it is characterized in that: reactor bottom is equipped with aeration head, adopt air aeration when waste water is flowed through reactor, air speed is 25~50L/h, utilizes air-flow to shear generation and the growth that promotes granule sludge.
CN2009100403641A 2009-06-19 2009-06-19 Culture method of aerobic denitrification particle sludge Expired - Fee Related CN101928067B (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103102007A (en) * 2013-02-06 2013-05-15 东北林业大学 Long-term storage method of aerobiotic nitrifying granule sludge
CN104129845A (en) * 2013-07-15 2014-11-05 成都信息工程学院 Novel method for culturing aerobic denitrification granule sludge in continuous flow system
CN113354080A (en) * 2021-06-10 2021-09-07 河北工程大学 Culture method and application of denitrification granular sludge

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100404436C (en) * 2005-06-03 2008-07-23 清华大学 Culture method of aerobic nitrosated granular sludge
CN100582027C (en) * 2006-07-07 2010-01-20 哈尔滨工业大学 Two-stage synchronous nitrification denitrification process for treating ammonia nitrogen wastewater

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103102007A (en) * 2013-02-06 2013-05-15 东北林业大学 Long-term storage method of aerobiotic nitrifying granule sludge
CN103102007B (en) * 2013-02-06 2014-09-10 东北林业大学 Long-term storage method of aerobiotic nitrifying granule sludge
CN104129845A (en) * 2013-07-15 2014-11-05 成都信息工程学院 Novel method for culturing aerobic denitrification granule sludge in continuous flow system
CN104129845B (en) * 2013-07-15 2017-02-08 成都信息工程大学 Novel method for culturing aerobic denitrification granule sludge in continuous flow system
CN113354080A (en) * 2021-06-10 2021-09-07 河北工程大学 Culture method and application of denitrification granular sludge

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