CN102453675B - Culture system of function strengthened microbe and directional concentration method - Google Patents
Culture system of function strengthened microbe and directional concentration method Download PDFInfo
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- CN102453675B CN102453675B CN 201010513960 CN201010513960A CN102453675B CN 102453675 B CN102453675 B CN 102453675B CN 201010513960 CN201010513960 CN 201010513960 CN 201010513960 A CN201010513960 A CN 201010513960A CN 102453675 B CN102453675 B CN 102453675B
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Abstract
The invention discloses a culture system of function strengthened microbe and a directional concentration method. The culture system comprises a nitrous acid bacterium culture bioreactor, a moving bed adsorption filtering apparatus coupled with the nitrous acid bacterium culture bioreactor, a denitrifying bacterium culture bioreactor and a moving bed adsorption filtering apparatus coupled with the denitrifying bacterium culture bioreactor; the moving bed adsorption filtering apparatuses recover microbial thalli brought out by corresponding bioreactors, and the recovered microbial thalli can be recycled to corresponding bioreactor or be discharged for sale or applied to a correlative sewage disposal system according to requirements. Compared with a prior art, the method of the invention can concentrate two thalli at one time and enhance microbial cultivation quality while reducing production energy consumption. The invention also has advantages of convenient operation, low energy consumption, saved cost and momentarily elution and collection of thalli, etc.
Description
Technical field
The invention belongs to the Biotechnology To Wastewater Treatment field, relate in particular to a kind of continuous culture system of denitrification microorganism, particularly relate to a kind of method of utilizing continuous culture system enrichment culture denitrification microorganism.
Background technology
Traditional sewage work's two stage biological treatment process does not design for the ammonia nitrogen in high density removal of pollutants usually, and is therefore lower to ammonia nitrogen removal efficient.The Process of Biological Nitrogen Removal that development in recent years is got up all is the good method that solves the ammonia and nitrogen pollution problem such as technology such as Anammox, short-cut nitrification and denitrification, synchronous nitration and denitrifications.Because the microorganism growth of degradation of ammonia nitrogen is slow, so to keep certain biomass be bio-denitrification technology in one of key link of industrial realization.
The microbial film water technology can make the higher biomass of maintenance in the reactor, but microbial film easily comes off and stops up filler when the operation fluctuation, causes the denitrification effect variation.The characteristics of the flush eddy bio-reactor that CN03279644 proposes are to be equipped with in reactor along the axial arranged spiral vortex plate of reactor, polymer carrier is housed between vortex board, this reactor can reduce and avoid filler to stop up, but operation impacted or the biofilm time is long, when the system suspended biomass is many, thalline still can run off in a large number.The membrane bioreactor (MBR) that membrane separation technique is combined with biological processing unit can solve thalline and outflow with water problem.But easily pollute for the film of holding back solid pollutant, the handling capacity of the contaminated rear water of film will decline to a great extent, and these problems are restricted membrane bioreactor in the industrial application of big yield.
The short-cut nitrification and denitrification bio-denitrification technology claims again the nitrite type bio-denitrification technology, exactly nitrifying process is controlled at NO
2-N stage and stopping, carry out subsequently nitrogen Removal by Denitrification from Nitrite.Compare with the traditional biological denitride technology, short distance nitration-denitrification biological denitrogenation technology can shorten hydraulic detention time, can save 25% energy consumption and 40% carbon source, can reduce the excess sludge treatment capacity simultaneously.Short distance nitration adopts the factors such as low dissolved axygen (DO), high temperature, high pH, supressor to control or screen the nitrosification dominant microflora at present, because condition changes short distance nitration can't effectively be controlled to the phenomenon that complete nitrification transforms; Short-cut denitrification mainly adopts the suspension activated sludge process, because it is low to be responsible for the sludge concentration of denitrification functions in the system, cause the total nitrogen removal effect undesirable, particularly when condition is controlled bad Sewage treatment systems and is subject to impacting, need to add certain biomass and repair fast.It is the suitable means that realize the short-cut nitrification and denitrification bio-denitrification technology that enrichment culture is responsible for denitrification microorganism.
The microorganism that is present in occurring in nature only has a very little part can obtain pure culture, and the micropopulation Body Effect of particularly being responsible for denitrogenation is stronger, and the functional effect after the pure culture is poor during than mixed culture; Namely allow to obtain the pure culture microorganism, the substrate that is applied to utilize in the engineering system is limited, a little less than the impact resistance, so for the denitrogenation flora, adopt non-pure culture system in specific environment, to carry out orienting enriching, can obtain the denitrification microorganism group that functional microorganism has comparative advantage.
At present existing multiple-microorganism culture apparatus, CN200710099489.2 discloses a kind of communication type microbial cultivation device and cultural method thereof of built-in microporous membrane, and utilize substratum to the microorganism in the various environment cultivate, separation and purification, and the technology of observing.CN200610057145.0 discloses a kind of microbial culture system and has used the method that this system carries out microorganism culturing, this system comprises heating, sterilization and the microorganism culturing culture tank for substratum, water treating equipment, aerating whipping device and automatic control equipment; In disclosed a kind of microbial culture method, microorganism culturing is carried out in this microbial culture garden.CN1324924A discloses the yeast culture apparatus and method and has adopted the yeast culture system of such device, is the magnetic coupling type yeast culture device that uses when carrying out enlarged culturing by test tube strains.
Above-mentioned existing apparatus and culture systems design mainly for separating the pure culture microorganism, yeast culture can only be carried out and this process of solid-liquid separation can not be finished, and the same time of said system can only be finished a kind of cultivation of flora, is unwell to finish simultaneously Nitrosomas and nitrite type denitrifying bacteria enrichment system and orienting enriching method.
Summary of the invention
Have in order to overcome microbe application that pure culture obtains that function is poor in engineering system, limited, the consuming time length of substrate utilized and can't obtain the deficiency such as function strengthened microflora, solve the enrichment culture problem of thalline in the short-cut nitrification and denitrification technological process, the present invention proposes a kind of can high-efficient culture and collect the continuous operational system of denitrification microorganism.
The culture systems of the function intensified microorganism of the present invention comprises Nitrosomas culture bioreactors, Nitrosomas moving-bed adsorption filtration device, denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device; Nitrosomas culture bioreactors water outlet communicates with Nitrosomas moving-bed adsorption filtration device entrance, Nitrosomas moving-bed adsorption filtration device purified liquor outlet communicates with denitrifying bacteria culture bioreactors water-in, and the concentrated suspension liquid outlet of Nitrosomas moving-bed adsorption filtration device communicates with Nitrosomas culture bioreactors and Nitrosomas retrieving arrangement respectively; Denitrifying bacteria culture bioreactors water outlet communicates with denitrifying bacteria moving-bed adsorption filtration device entrance, and the concentrated suspension liquid outlet of denitrifying bacteria moving-bed adsorption filtration device communicates with denitrifying bacteria culture bioreactors and denitrifying bacteria retrieving arrangement respectively.
Among the present invention, the Nitrosomas culture bioreactors can adopt any bio-aeration reactor in this area, can use filler in the reactor, also can not use filler.The denitrifying bacteria culture bioreactors can adopt any stirred bioreactor in this area, can use filler in the reactor, also can not use filler.Two bio-reactors preferably adopt the operate continuously mode.
Among the present invention, Nitrosomas moving-bed adsorption filtration device and denitrifying bacteria moving-bed adsorption filtration device can adopt the moving-bed filtration unit of operate continuously, suspended substance adsorption filtration during the moving-bed filtration unit will be intake obtains clear liquid, the suspended substance that the filler of simultaneously cleaning absorption suspended substance obtains concentrating.Concrete such as described Continuous Moving Bed filtration units such as CN03134006.7, CN03134007.5, CN03213521.1, certainly, can use the thalline of can realizing arbitrarily suspending to filter the filtration unit of collecting.
Among the present invention, corresponding Controlling System can be set as required, power system, thalline packaging system etc.
The orienting enriching method of the function intensified microorganism of the present invention, use above-mentioned culture systems, in Nitrosomas culture bioreactors and Nitrosomas moving-bed adsorption filtration device, cultivate Nitrosomas, in denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device, cultivate denitrifying bacteria.The processing condition of Nitrosomas culture bioreactors are: 15 ℃～40 ℃ of temperature, preferred 20 ℃～35 ℃; PH6～9.5, preferred 7.5～8.5; Dissolved oxygen 0.2mgL
-1～7.0mgL
-1, preferred 0.5mgL
-1～2.5mgL
-1The initial ammonia nitrogen concentration of nutrient solution is 200～1500mg/L in the system, and COD concentration is lower than 200mg/L, can also contain a small amount of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metal ion and phosphate anion.
The processing condition of denitrifying bacteria culture bioreactors are: 15 ℃～40 ℃, preferred 20～35 ℃ of temperature; PH is 6.0～10.0 preferred 7.8～9.0; Dissolved oxygen is less than 1.0mgL
-1The initial total nitrogen concentration of nutrient solution is 200～1500mg/L in the system, and wherein nitrite nitrogen accounts for 75%～85% of total nitrogen concentration, and COD concentration is 600～4500mg/L.Can also contain a small amount of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metal ion and phosphate anion.
A kind of idiographic flow of the orienting enriching method of the function intensified microorganism of the present invention is: ammonia-containing water is at first squeezed into the Nitrosomas culture bioreactors, under aerobic condition, carry out the Nitrosomas enrichment culture, nitrated water outlet after the cultivation enters Nitrosomas moving-bed adsorption filtration device from top, nitrococcus is trapped collection, nitrated water outlet after the adsorption filtration enters the enrichment culture that the denitrifying bacteria culture bioreactors is carried out denitrifying bacteria, denitrification water outlet after the processing enters denitrifying bacteria moving-bed adsorption filtration device, and denitrifying bacteria is trapped collection.Nitrococcus or the denitrifying bacteria collected loop back corresponding biochemical reactor, and the thalline of continuous or periodic separate part enrichment culture effluxes packing as required simultaneously, obtains corresponding function enhancement microbiological thalline, are used for suitable wastewater biochemical treating processes.
In the inventive method, the initial source of cultivating nitrosification flora and denitrification flora can be by the matrix that contains nitrobacteria.The matrix that contains nitrobacteria and denitrifying bacteria derives from natural soil, mud or contains the active sludge of ammonia industrial effluent treatment plant, or other all be rich in the matrix of nitrobacteria or denitrifying bacteria.
The nitrosification dominant microflora that the inventive method obtains has higher ammonia nitrogen removal frank and nitrite generating rate, the denitrifying bacteria that obtains has higher nitrogen removal rate, two kinds of floras have stronger tolerance and adaptability, have preferably shock resistance and stability; The microflora of enrichment can be directly used in ammonia-containing water and process or replenish as microorganism after system is impacted, and plays the effect of quick reparation.
The culture systems of the function intensified microorganism of the present invention and orienting enriching method with bio-reactor and one can cyclic regeneration the adsorption filtration device coupling, the adsorption filtration device volume is little, can be in the absorption thalline with the denitrification microorganism that the absorbs foldback thing reactor of bringing back to life continuously, the high-density thalline has improved enriching rate, effectively the flora of required specific function is strengthened simultaneously.Use the purpose that this system can reach two kinds of thalline of Sync enrichment, when improving the microorganism culturing quality, reach the reduction production energy consumption.The present invention has easy to operate, and energy consumption is low, saves cost, can carry out at any time the advantages such as wash-out collection to thalline.
Description of drawings
Fig. 1 is the inventive method general flow chart.
Embodiment
Further specify the solution of the present invention and effect below by embodiment.
Among the embodiment, the Nitrosomas culture bioreactors is the full back-mixed reactors of continuously feeding formula, internal circulation biological reactor, helical flow bio-reactor.The denitrifying bacteria culture bioreactors is continuous stirred tank reactor.Nitrosomas moving-bed adsorption filtration device and denitrifying bacteria moving-bed adsorption filtration device are the described Continuous Moving Bed filtration unit of CN03134007.5.The initial source of nitrosification flora and denitrification flora is Fushun No.3 Petroleum Factory's sludge sewage.
Embodiment 1
Nitrosomas is cultivated and is adopted the full back-mixed reactors of continuously feeding formula, and 25 ℃ of temperature, pH 7.8, dissolved oxygen 1.2mgL
-1Condition under carry out Nitrosomas and cultivate, the initial ammonia nitrogen concentration of nutrient solution is 400mg/L, COD concentration is lower than 80mg/L, also contains a small amount of Fe in the nutrient solution
2+, Mg
2-, K
+, Ca
2+Deng metal ion and phosphate anion; Denitrifying bacteria is cultivated and is adopted continuous stirred tank reactor, and 25 ℃ of temperature, pH is 8.0～8.5, and dissolved oxygen is less than 0.2mgL
-1Condition under carry out anti-Nitromonas and cultivate, the initial nitrite nitrogen concentration of nutrient solution is 380mg/L, COD concentration is 1050mg/L, also contains a small amount of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metal ion and phosphate anion.Adopt the Continuous Moving Bed filtration unit to carry out the orienting enriching of function intensified microorganism, the average growth rate of two kinds of thalline is 0.113g/ (Ld) and 0.152g/ (Ld), cultivate and gather in the crops liquid Nitrosomas 400L, liquid denitrifying bacteria 300L after 20 days, Nitrosomas and denitrifying bacteria have increased respectively 100 times and 135 times.
Embodiment 2
Adopt internal circulation biological reactor 30 ℃ of temperature, pH 8.0, dissolved oxygen 0.5mgL
-1Carry out Nitrosomas under the condition and cultivate, the initial ammonia nitrogen concentration of nutrient solution is 800mg/L, and COD concentration is lower than 150mg/L, also contains a small amount of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metal ion and phosphate anion; Adopt continuous stirred tank reactor 30 ℃ of temperature, pH is 8.0～9.0, and dissolved oxygen is less than 0.5mgL
-1Carry out denitrifying bacteria under the condition and cultivate, the initial nitrite nitrogen concentration of nutrient solution is 780mg/L, and COD concentration is 2050mg/L, also contains a small amount of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metal ion and phosphate anion.Adopt the Continuous Moving Bed filtration unit to carry out the orienting enriching of function intensified microorganism, the growth velocity average out to 0.152g/ (Ld) of two kinds of thalline and 0.182g/ (Ld), cultivate and gather in the crops liquid Nitrosomas 200L, liquid denitrifying bacteria 200L after 30 days, Nitrosomas and denitrifying bacteria have increased respectively 150 times and 180 times.
Embodiment 3
Adopt spiral bio-reactor 35 ℃ of temperature, pH 7.5, dissolved oxygen 2.0mgL
-1Carry out Nitrosomas under the condition and cultivate, the initial ammonia nitrogen concentration of nutrient solution is 1200mg/L, and COD concentration is lower than 250mg/L, also contains a small amount of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metal ion and phosphate anion; Adopt continuous stirred tank reactor 35 ℃ of temperature, pH is 8.0, and dissolved oxygen is less than 0.1mgL
-1Carrying out denitrifying bacteria under the condition cultivates; The initial nitrite nitrogen concentration of nutrient solution is 1100mg/L, and COD concentration is 3050mg/L, also contains a small amount of Fe in the nutrient solution
2+, Mg
2-, K
+, Ca
2+Deng metal ion and phosphate anion.Adopt the Continuous Moving Bed filtration unit to carry out the orienting enriching of function intensified microorganism.Cultivate and gather in the crops liquid Nitrosomas 200L, liquid denitrifying bacteria 200L after 15 days, Nitrosomas and denitrifying bacteria have increased respectively 60 times and 80 times.
Embodiment 4
Adopt A/O technique, use the thalline of embodiment 1～3 enrichment processing the ammonia-containing water that produces in the urea production process under the operational conditions continuously, the ammonia nitrogen concentration in the waste water is 400～600mg/L.Wherein aerobic reactor is to add Nitrosomas about 2000mg/L according to sludge concentration, and hydraulic detention time is less than 24h, and aerobic processes control temperature of reaction is 25 ℃～35 ℃, and pH is controlled between 7.8～8.5, and dissolved oxygen is controlled at 0.5mgL
- 1～2.5mgL
-1Anaerobic reactor is to add denitrifying bacteria about 1500mg/L according to sludge concentration, and hydraulic detention time is less than 24h, and temperature is 25 ℃～30 ℃ in the reaction process, and pH is controlled between 8.0～9.0, and dissolved oxygen is less than 0.2mgL
-1The nitrated stage is replenished basicity with sodium bicarbonate in the treating processes, the denitrification stage with methyl alcohol as carbon source.Effect after the processing sees Table 1, and as can be seen from Table 1, ammonia nitrogen removal frank reaches more than 90% during the Nitrosomas Ammonia-Containing Wastewater Treatment of embodiment 1～3 enrichment, and the nitrous rate reaches more than 75%, and the denitrifying bacteria nitrogen removal rate of enrichment reaches more than 85%.
The treatment effect of table 1 enrichment thalline
Claims (15)
1. the culture systems of a function intensified microorganism is characterized in that: comprise Nitrosomas culture bioreactors, Nitrosomas moving-bed adsorption filtration device, denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device; Nitrosomas culture bioreactors water outlet communicates with Nitrosomas moving-bed adsorption filtration device entrance, Nitrosomas moving-bed adsorption filtration device purified liquor outlet communicates with denitrifying bacteria culture bioreactors water-in, and the concentrated suspension liquid outlet of Nitrosomas moving-bed adsorption filtration device communicates with Nitrosomas culture bioreactors and Nitrosomas retrieving arrangement respectively; Denitrifying bacteria culture bioreactors water outlet communicates with denitrifying bacteria moving-bed adsorption filtration device entrance, and the concentrated suspension liquid outlet of denitrifying bacteria moving-bed adsorption filtration device communicates with denitrifying bacteria culture bioreactors and denitrifying bacteria retrieving arrangement respectively.
2. according to culture systems claimed in claim 1, it is characterized in that: the Nitrosomas culture bioreactors adopts the bio-aeration reactor, and the denitrifying bacteria culture bioreactors adopts stirred bioreactor.
3. according to claim 1 or 2 described culture systems, it is characterized in that: Nitrosomas culture bioreactors and denitrifying bacteria culture bioreactors adopt the operate continuously mode.
4. according to culture systems claimed in claim 1, it is characterized in that: Nitrosomas moving-bed adsorption filtration device and denitrifying bacteria moving-bed adsorption filtration device adopt the moving-bed filtration unit of operate continuously, suspended substance adsorption filtration during the moving-bed filtration unit will be intake obtains clear liquid, the suspended substance that the filler of simultaneously cleaning absorption suspended substance obtains concentrating.
5. according to culture systems claimed in claim 1, it is characterized in that: Controlling System is set, power system and thalline packaging system in the culture systems.
6. the orienting enriching method of a function intensified microorganism, it is characterized in that right to use requires the described culture systems of 1 to 5 arbitrary claim, in Nitrosomas culture bioreactors and Nitrosomas moving-bed adsorption filtration device, cultivate Nitrosomas, in denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device, cultivate denitrifying bacteria.
7. it is characterized in that in accordance with the method for claim 6: the processing condition of Nitrosomas culture bioreactors are: temperature is 15 ℃～40 ℃; The pH value is 6～9.5; Dissolved oxygen is 0.2mgL
-1～7.0mgL
- 1
8. according to claim 6 or 7 described methods, it is characterized in that: in the Nitrosomas culture bioreactors culture system, the initial ammonia nitrogen concentration of nutrient solution is 200～1500mg/L, and COD concentration is lower than 200mg/L.
9. it is characterized in that in accordance with the method for claim 6: the processing condition of denitrifying bacteria culture bioreactors are: temperature is 15 ℃～40 ℃; The pH value is 6.0～10.0; Dissolved oxygen is less than 1.0mgL
-1
10. according to claim 6 or 9 described methods, it is characterized in that: in the denitrifying bacteria culture bioreactors culture system, the initial total nitrogen concentration of nutrient solution is 200～1500mg/L, and wherein nitrite nitrogen accounts for 75%～85% of total nitrogen concentration, and COD concentration is 600～4500mg/L.
11. it is characterized in that in accordance with the method for claim 7: the temperature of Nitrosomas culture bioreactors is 20 ℃～35 ℃.
12. it is characterized in that in accordance with the method for claim 7: the pH value of Nitrosomas culture bioreactors is 7.5～8.5.
13. it is characterized in that in accordance with the method for claim 7: the dissolved oxygen of Nitrosomas culture bioreactors is 0.5mgL
-1～2.5mgL
-1
14. it is characterized in that in accordance with the method for claim 9: the temperature of denitrifying bacteria culture bioreactors is 20～35 ℃.
15. it is characterized in that in accordance with the method for claim 9: the pH value of denitrifying bacteria culture bioreactors is 7.8～9.0.
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| CN103194381B (en) * | 2013-04-03 | 2014-08-06 | 天津市兴源环境技术工程有限公司 | Screening and identification kit of denitrifying bacteria and screening and identification method |
| CN106754453B (en) * | 2015-11-19 | 2020-08-07 | 中国石油化工股份有限公司 | Enrichment culture method of function-enhanced denitrification microorganisms |
| CN109182211B (en) * | 2018-10-16 | 2021-09-07 | 中国石油化工股份有限公司 | Composite biological agent for preventing and treating SRB in wide-temperature environment and use method |
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