CN102453675A - Culture system of function strengthened microbe and directional concentration method - Google Patents
Culture system of function strengthened microbe and directional concentration method Download PDFInfo
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- CN102453675A CN102453675A CN201010513960XA CN201010513960A CN102453675A CN 102453675 A CN102453675 A CN 102453675A CN 201010513960X A CN201010513960X A CN 201010513960XA CN 201010513960 A CN201010513960 A CN 201010513960A CN 102453675 A CN102453675 A CN 102453675A
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Abstract
The invention discloses a culture system of function strengthened microbe and a directional concentration method. The culture system comprises a nitrous acid bacterium culture bioreactor, a moving bed adsorption filtering apparatus coupled with the nitrous acid bacterium culture bioreactor, a denitrifying bacterium culture bioreactor and a moving bed adsorption filtering apparatus coupled with the denitrifying bacterium culture bioreactor; the moving bed adsorption filtering apparatuses recover microbial thalli brought out by corresponding bioreactors, and the recovered microbial thalli can be recycled to corresponding bioreactor or be discharged for sale or applied to a correlative sewage disposal system according to requirements. Compared with a prior art, the method of the invention can concentrate two thalli at one time and enhance microbial cultivation quality while reducing production energy consumption. The invention also has advantages of convenient operation, low energy consumption, saved cost and momentarily elution and collection of thalli, etc.
Description
Technical field
The invention belongs to the WWT biological technical field, relate in particular to a kind of continuous culture system of denitrification microorganism, particularly relate to a kind of method of utilizing continuous culture system enrichment culture denitrification microorganism.
Background technology
Traditional sewage work's two stage biological treatment process is not directed against the removal of high strength ammonia nitrogen pollutant usually and designs, and is therefore lower to ammonia nitrogen removal efficient.The new bio denitrification process that development in recent years is got up all is the good method that solves the ammonia and nitrogen pollution problem like technology such as Anammox, short-cut nitrification and denitrification, synchronous nitration and denitrifications.Because the microorganism growth of degradation of ammonia nitrogen is slow, be one of key link of in industry, realizing of bio-denitrification technology so keep certain living weight.
The microbial film water technology can make the higher living weight of maintenance in the reactor drum, but microbial film is prone to come off and stop up filler when the operation fluctuation, causes the denitrification effect variation.The characteristics of the eddy current bio-reactor of rising as high as the banks that CN03279644 proposes are in reactor drum, to be equipped with along the axial arranged spiral vortex plate of reactor drum; Polymer carrier is housed between vortex board; This reactor drum can reduce and avoid filler to stop up; But impacted or hang long, system suspended biomass of film time for a long time in operation, thalline still can run off in a large number.The membrane bioreactor (MBR) that membrane separation technique is combined with biological processing unit can solve thalline and lose problem with current.But the film that is used to hold back solid pollutant pollutes easily, and the handling capacity of the contaminated back of film water will decline to a great extent, and these problems are restricted membrane bioreactor in the industrial application of big yield.
The short-cut nitrification and denitrification bio-denitrification technology is claimed the nitrite type bio-denitrification technology again, exactly nitrifying process is controlled at NO
2-N stage and stopping, carry out the nitrite denitrification denitrogenation subsequently.Compare with the traditional biological denitride technology, short distance nitration-denitrification biological denitrogenation technology can shorten hydraulic detention time, can save 25% energy consumption and 40% carbon source, can reduce the excess sludge treatment capacity simultaneously.Short distance nitration adopts factors such as LDO (DO), high temperature, high pH, supressor to control or screen the nitrosification dominant microflora at present, because condition changes short distance nitration can't effectively be controlled to the phenomenon that complete nitrification transforms; Short-cut denitrification mainly adopts the suspension activated sludge process; Because it is low to be responsible for the sludge concentration of denitrification functions in the system; Cause the total nitrogen removal effect undesirable, particularly control bad Sewage treatment systems and receive when impacting, need add certain living weight and repair fast when condition.It is the suitable means that realize the short-cut nitrification and denitrification bio-denitrification technology that enrichment culture is responsible for denitrification microorganism.
The mikrobe that is present in occurring in nature has only a very little part can obtain pure culture, and the micropopulation gunn of particularly being responsible for denitrogenation is stronger, and the functional effect after the pure culture is poor during than mixed culture; Even can access the pure culture mikrobe; The substrate that is applied to utilize in the engineering system is limited, a little less than the impact resistance, so for the denitrogenation flora; Adopt non-pure culture system in particular environment, to carry out orienting enriching, can obtain the denitrification microorganism group that functional microorganism has comparative advantage.
At present existing multiple microbial cultivation device; CN200710099489.2 discloses a kind of communication type microbial cultivation device and cultural method thereof of built-in microporous membrane; And utilize substratum to the mikrobe in the various environment cultivate, separation and purification, and the technology of observing.CN200610057145.0 discloses a kind of microorganism culturing system and has used the method that this system carries out microorganism culturing; This system comprises heating, sterilization and the microorganism culturing culture tank that is used for substratum; Water treating equipment, aerating whipping device and automatic control equipment; In disclosed a kind of microbial culture method, microorganism culturing is carried out in this microorganism culturing device.CN1324924A discloses the yeast culture apparatus and method and has adopted the yeast culture system of this type of device, is the magnetic coupling type yeast culture device that uses when carrying out enlarged culturing by test tube strains.
Above-mentioned existing apparatus and culture systems are primarily aimed at the separation of pure culturing micro-organisms and design; Yeast culture can only be carried out and this process of solid-liquid separation can not be accomplished; And the same time of said system can only be accomplished a kind of cultivation of flora, is unwell to accomplish Nitrosomas and nitrite type denitrifying bacteria enrichment system and orienting enriching method simultaneously.
Summary of the invention
Be applied to exist in the engineering system in order to overcome mikrobe that pure culture obtains that function is poor, limited, the consuming time length of the substrate of utilization and can't obtain deficiency such as function strengthened microflora; Solve the enrichment culture problem of thalline in the short-cut nitrification and denitrification technological process, the present invention proposes a kind of continuous operational system of can high-efficient culture and collecting denitrification microorganism.
The culture systems of the function intensified mikrobe of the present invention comprises Nitrosomas culture bioreactors, Nitrosomas moving-bed adsorption filtration device, denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device; Nitrosomas culture bioreactors water outlet communicates with Nitrosomas moving-bed adsorption filtration device inlet; Nitrosomas moving-bed adsorption filtration device purified liquor outlet communicates with denitrifying bacteria culture bioreactors water-in, and the concentrated suspension liquid outlet of Nitrosomas moving-bed adsorption filtration device communicates with Nitrosomas culture bioreactors and Nitrosomas retrieving arrangement respectively; Denitrifying bacteria culture bioreactors water outlet communicates with denitrifying bacteria moving-bed adsorption filtration device inlet, and the concentrated suspension liquid outlet of denitrifying bacteria moving-bed adsorption filtration device communicates with denitrifying bacteria culture bioreactors and denitrifying bacteria retrieving arrangement respectively.
Among the present invention, the Nitrosomas culture bioreactors can adopt any bio-aeration reactor drum in this area, can use filler in the reactor drum, also can not use filler.The denitrifying bacteria culture bioreactors can adopt any stirred bioreactor in this area, can use filler in the reactor drum, also can not use filler.Two bio-reactors preferably adopt the operate continuously mode.
Among the present invention; Nitrosomas moving-bed adsorption filtration device and denitrifying bacteria moving-bed adsorption filtration device can adopt the moving-bed filtration unit of operate continuously; Suspended substance adsorption filtration during the moving-bed filtration unit will be intake obtains clear liquid, and the filler that cleans the absorption suspended substance simultaneously obtains spissated suspended substance.Concrete like described continuous moving-bed filtration units such as CN03134006.7, CN03134007.5, CN03213521.1, certainly, can use the thalline of can realizing arbitrarily suspending to filter the filtration unit of collecting.
Among the present invention, the control corresponding system can be set as required, power system, thalline packaging system etc.
The orienting enriching method of the function intensified mikrobe of the present invention; Use above-mentioned culture systems; In Nitrosomas culture bioreactors and Nitrosomas moving-bed adsorption filtration device, cultivate Nitrosomas, in denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device, cultivate denitrifying bacteria.The processing condition of Nitrosomas culture bioreactors are: 15 ℃～40 ℃ of temperature, preferred 20 ℃～35 ℃; PH6～9.5, preferred 7.5～8.5; Dissolved oxygen 0.2mgL
-1～7.0mgL
-1, preferred 0.5mgL
-1～2.5mgL
-1The initial ammonia nitrogen concentration of nutrient solution is 200～1500mg/L in the system, and COD concentration is lower than 200mg/L, can also contain a spot of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metals ion and phosphate anion.
The processing condition of denitrifying bacteria culture bioreactors are: 15 ℃～40 ℃, preferred 20～35 ℃ of temperature; PH is 6.0～10.0 preferred 7.8～9.0; Dissolved oxygen is less than 1.0mgL
-1The initial total nitrogen concentration of nutrient solution is 200～1500mg/L in the system, and wherein nitrite nitrogen accounts for 75%～85% of total nitrogen concentration, and COD concentration is 600～4500mg/L.Can also contain a spot of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metals ion and phosphate anion.
A kind of idiographic flow of the orienting enriching method of the function intensified mikrobe of the present invention is: ammonia-containing water is at first squeezed into the Nitrosomas culture bioreactors; Under aerobic condition, carry out the Nitrosomas enrichment culture; Nitrated water outlet after the cultivation gets into Nitrosomas moving-bed adsorption filtration device from top; Nitrococcus is trapped collection; Nitrated water outlet after the adsorption filtration gets into the enrichment culture that the denitrifying bacteria culture bioreactors is carried out denitrifying bacteria, and the denitrification water outlet after the processing gets into denitrifying bacteria moving-bed adsorption filtration device, and denitrifying bacteria is trapped collection.Nitrococcus or the denitrifying bacteria collected loop back the relevant biochemical reactor drum, and the thalline of continuous or periodic as required separate part enrichment culture effluxes packing simultaneously, obtains corresponding function enhancement microbiological thalline, are used for suitable wastewater biochemical treating processes.
In the inventive method, the initial source of cultivating nitrosification flora and denitrification flora can be by the matrix that contains nitrobacteria.The matrix that contains nitrobacteria and denitrifying bacteria derives from natural soil, mud or contains the active sludge of ammonia industrial effluent treatment plant, or other all be rich in the matrix of nitrobacteria or denitrifying bacteria.
The nitrosification dominant microflora that the inventive method obtains has higher ammonia nitrogen removal frank and nitrite generating rate; The denitrifying bacteria that obtains has higher nitrogen removal rate; Two kinds of floras have stronger tolerance and flexibility, have shock resistance and stability preferably; The microflora of enrichment can be used for directly that ammonia-containing water is handled or receive in system and to impact the back and replenish as mikrobe, plays the effect of quick reparation.
Culture systems of the function intensified mikrobe of the present invention and orienting enriching method with bio-reactor and one can cyclic regeneration the adsorption filtration device coupling; The adsorption filtration device volume is little; Can be in the absorption thalline with the denitrification microorganism that the absorbs foldback thing reactor drum of bringing back to life continuously; The high-density thalline has improved enriching rate, effectively the flora of required specific function is strengthened simultaneously.Use the purpose that this system can reach two kinds of thalline of enrichment simultaneously, when improving the microorganism culturing quality, reach the reduction production energy consumption.The present invention has easy to operate, and energy consumption is low, practices thrift cost, can carry out advantages such as wash-out collection at any time to thalline.
Description of drawings
Fig. 1 is the inventive method general flow chart.
Embodiment
Further specify scheme of the present invention and effect through embodiment below.
Among the embodiment, the Nitrosomas culture bioreactors is the full back-mixed reactors of continuously feeding formula, internal circulation biological reactor, helical flow bio-reactor.The denitrifying bacteria culture bioreactors is a continuous stirred tank reactor.Nitrosomas moving-bed adsorption filtration device and denitrifying bacteria moving-bed adsorption filtration device are the described continuous moving-bed filtration unit of CN03134007.5.The initial source of nitrosification flora and denitrification flora is Fushun No.3 Petroleum Factory's sludge sewage.
Embodiment 1
Nitrosomas is cultivated and is adopted the full back-mixed reactors of continuously feeding formula, and 25 ℃ of temperature, pH 7.8, dissolved oxygen 1.2mgL
-1Condition under carry out Nitrosomas and cultivate, the initial ammonia nitrogen concentration of nutrient solution is 400mg/L, COD concentration is lower than 80mg/L, also contains a spot of Fe in the nutrient solution
2+, Mg
2-, K
+, Ca
2+Deng metals ion and phosphate anion; Denitrifying bacteria is cultivated and is adopted continuous stirred tank reactor, and 25 ℃ of temperature, pH is 8.0～8.5, and dissolved oxygen is less than 0.2mgL
-1Condition under carry out anti-Nitromonas and cultivate, the initial nitrite nitrogen concentration of nutrient solution is 380mg/L, COD concentration is 1050mg/L, also contains a spot of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metals ion and phosphate anion.Adopt continuous moving-bed filtration unit to carry out the orienting enriching of function intensified mikrobe; The average growth velocity of two kinds of thalline is 0.113g/ (Ld) and 0.152g/ (Ld); Cultivate results liquid Nitrosomas 400L, liquid denitrifying bacteria 300L after 20 days, Nitrosomas and denitrifying bacteria have increased by 100 times and 135 times respectively.
Embodiment 2
Adopt internal circulation biological reactor 30 ℃ of temperature, pH 8.0, dissolved oxygen 0.5mgL
-1Carry out Nitrosomas under the condition and cultivate, the initial ammonia nitrogen concentration of nutrient solution is 800mg/L, and COD concentration is lower than 150mg/L, also contains a spot of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metals ion and phosphate anion; Adopt continuous stirred tank reactor 30 ℃ of temperature, pH is 8.0～9.0, and dissolved oxygen is less than 0.5mgL
-1Carry out denitrifying bacteria under the condition and cultivate, the initial nitrite nitrogen concentration of nutrient solution is 780mg/L, and COD concentration is 2050mg/L, also contains a spot of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metals ion and phosphate anion.Adopt continuous moving-bed filtration unit to carry out the orienting enriching of function intensified mikrobe; The growth velocity average out to 0.152g/ (Ld) of two kinds of thalline and 0.182g/ (Ld); Cultivate results liquid Nitrosomas 200L, liquid denitrifying bacteria 200L after 30 days, Nitrosomas and denitrifying bacteria have increased by 150 times and 180 times respectively.
Embodiment 3
Adopt spiral bio-reactor 35 ℃ of temperature, pH 7.5, dissolved oxygen 2.0mgL
-1Carry out Nitrosomas under the condition and cultivate, the initial ammonia nitrogen concentration of nutrient solution is 1200mg/L, and COD concentration is lower than 250mg/L, also contains a spot of Fe in the nutrient solution
2+, Mg
2+, K
+, Ca
2+Deng metals ion and phosphate anion; Adopt continuous stirred tank reactor 35 ℃ of temperature, pH is 8.0, and dissolved oxygen is less than 0.1mgL
-1Carrying out denitrifying bacteria under the condition cultivates; The initial nitrite nitrogen concentration of nutrient solution is 1100mg/L, and COD concentration is 3050mg/L, also contains a spot of Fe in the nutrient solution
2+, Mg
2-, K
+, Ca
2+Deng metals ion and phosphate anion.Adopt continuous moving-bed filtration unit to carry out the orienting enriching of function intensified mikrobe.Cultivate results liquid Nitrosomas 200L, liquid denitrifying bacteria 200L after 15 days, Nitrosomas and denitrifying bacteria have increased by 60 times and 80 times respectively.
Embodiment 4
Adopt A/O technology, use the thalline of embodiment 1～3 enrichment handling the ammonia-containing water that produces in the urea production process under the operational conditions continuously, the ammonia nitrogen concentration in the waste water is 400～600mg/L.Wherein aerobic reactor is to add Nitrosomas about 2000mg/L according to sludge concentration, and hydraulic detention time is less than 24h, and the aerobic processes control reaction temperature is 25 ℃～35 ℃, and pH is controlled between 7.8～8.5, and dissolved oxygen is controlled at 0.5mgL
- 1～2.5mgL
-1Anaerobic reactor is to add denitrifying bacteria about 1500mg/L according to sludge concentration, and hydraulic detention time is less than 24h, and temperature is 25 ℃～30 ℃ in the reaction process, and pH is controlled between 8.0～9.0, and dissolved oxygen is less than 0.2mgL
-1The nitrated stage is replenished basicity with sodium hydrogencarbonate in the treating processes, the denitrification stage with methyl alcohol as carbon source.Effect after the processing is seen table 1, can find out from table 1, and ammonia nitrogen removal frank reaches more than 90% during the Nitrosomas Ammonia-Containing Wastewater Treatment of embodiment 1～3 enrichment, and the nitrosification rate reaches more than 75%, and the denitrifying bacteria nitrogen removal rate of enrichment reaches more than 85%.
The treatment effect of table 1 enrichment thalline
Claims (10)
1. the culture systems of a function intensified mikrobe is characterized in that: comprise Nitrosomas culture bioreactors, Nitrosomas moving-bed adsorption filtration device, denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device; Nitrosomas culture bioreactors water outlet communicates with Nitrosomas moving-bed adsorption filtration device inlet; Nitrosomas moving-bed adsorption filtration device purified liquor outlet communicates with denitrifying bacteria culture bioreactors water-in, and the concentrated suspension liquid outlet of Nitrosomas moving-bed adsorption filtration device communicates with Nitrosomas culture bioreactors and Nitrosomas retrieving arrangement respectively; Denitrifying bacteria culture bioreactors water outlet communicates with denitrifying bacteria moving-bed adsorption filtration device inlet, and the concentrated suspension liquid outlet of denitrifying bacteria moving-bed adsorption filtration device communicates with denitrifying bacteria culture bioreactors and denitrifying bacteria retrieving arrangement respectively.
2. according to the described culture systems of claim 1, it is characterized in that: the Nitrosomas culture bioreactors adopts the bio-aeration reactor drum, and the denitrifying bacteria culture bioreactors adopts stirred bioreactor.
3. according to claim 1 or 2 described culture systems, it is characterized in that: Nitrosomas culture bioreactors and denitrifying bacteria culture bioreactors adopt the operate continuously mode.
4. according to the described culture systems of claim 1; It is characterized in that: Nitrosomas moving-bed adsorption filtration device and denitrifying bacteria moving-bed adsorption filtration device adopt the moving-bed filtration unit of operate continuously; Suspended substance adsorption filtration during the moving-bed filtration unit will be intake obtains clear liquid, and the filler that cleans the absorption suspended substance simultaneously obtains spissated suspended substance.
5. according to the described culture systems of claim 1, it is characterized in that: system is set, power system and thalline packaging system in the culture systems.
6. the orienting enriching method of a function intensified mikrobe; It is characterized in that using the described culture systems of the arbitrary claim of claim 1 to 5; In Nitrosomas culture bioreactors and Nitrosomas moving-bed adsorption filtration device, cultivate Nitrosomas, in denitrifying bacteria culture bioreactors and denitrifying bacteria moving-bed adsorption filtration device, cultivate denitrifying bacteria.
7. according to the described method of claim 6, it is characterized in that: the processing condition of Nitrosomas culture bioreactors are: 15 ℃～40 ℃ of temperature, preferred 20 ℃～35 ℃; PH6～9.5, preferred 7.5～8.5; Dissolved oxygen 0.2mgL
-1～7.0mgL
-1, preferred 0.5mgL
-1～2.5mgL
-1
8. according to claim 6 or 7 described methods, it is characterized in that: in the Nitrosomas culture bioreactors culture system, the initial ammonia nitrogen concentration of nutrient solution is 200～1500mg/L, and COD concentration is lower than 200mg/L.
9. according to the described method of claim 6, it is characterized in that: the processing condition of denitrifying bacteria culture bioreactors are: 15 ℃～40 ℃, preferred 20～35 ℃ of temperature; PH is 6.0～10.0 preferred 7.8～9.0; Dissolved oxygen is less than 1.0mgL
-1
10. according to claim 6 or 9 described methods; It is characterized in that: in the denitrifying bacteria culture bioreactors culture system; The initial total nitrogen concentration of nutrient solution is 200～1500mg/L, and wherein nitrite nitrogen accounts for 75%～85% of total nitrogen concentration, and COD concentration is 600～4500mg/L.
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| CN103194381A (en) * | 2013-04-03 | 2013-07-10 | 天津市兴源环境技术工程有限公司 | Screening and identification kit of denitrifying bacteria and screening and identification method |
| CN106754453A (en) * | 2015-11-19 | 2017-05-31 | 中国石油化工股份有限公司 | A kind of Enrichment culture method of function intensified denitrification microorganism |
| CN109182211A (en) * | 2018-10-16 | 2019-01-11 | 中国石油化工股份有限公司 | The composite bacteria agent and application method of SRB are prevented and treated under wide temperature environment |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109182211A (en) * | 2018-10-16 | 2019-01-11 | 中国石油化工股份有限公司 | The composite bacteria agent and application method of SRB are prevented and treated under wide temperature environment |
| CN109182211B (en) * | 2018-10-16 | 2021-09-07 | 中国石油化工股份有限公司 | Composite biological agent for preventing and treating SRB in wide-temperature environment and use method |
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