CN111183221B - 用于o-糖蛋白的蛋白酶和结合多肽 - Google Patents

用于o-糖蛋白的蛋白酶和结合多肽 Download PDF

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Publication number
CN111183221B
CN111183221B CN201880050251.7A CN201880050251A CN111183221B CN 111183221 B CN111183221 B CN 111183221B CN 201880050251 A CN201880050251 A CN 201880050251A CN 111183221 B CN111183221 B CN 111183221B
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CN111183221A (zh
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弗雷德里克·里奥
罗尔夫·卢德
史蒂芬·比约克
马林·梅贾尔
弗雷德里克·奥尔森
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Genovis AB
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Genovis AB
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Priority claimed from GBGB1708471.6A external-priority patent/GB201708471D0/en
Priority claimed from GBGB1708476.5A external-priority patent/GB201708476D0/en
Priority claimed from GBGB1806655.5A external-priority patent/GB201806655D0/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24057—O-Sialoglycoprotein endopeptidase (3.4.24.57), i.e. glycoprotease
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/952—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from bacteria

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
CN201880050251.7A 2017-05-26 2018-05-25 用于o-糖蛋白的蛋白酶和结合多肽 Active CN111183221B (zh)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB1708476.5 2017-05-26
GB1708471.6 2017-05-26
GBGB1708471.6A GB201708471D0 (en) 2017-05-26 2017-05-26 Polypeptide
GBGB1708476.5A GB201708476D0 (en) 2017-05-26 2017-05-26 Tools for glycan analysis
GB1806655.5 2018-04-24
GBGB1806655.5A GB201806655D0 (en) 2018-04-24 2018-04-24 Polypeptide
PCT/EP2018/063832 WO2018215656A1 (en) 2017-05-26 2018-05-25 Protease and binding polypeptide for o-glycoproteins

Publications (2)

Publication Number Publication Date
CN111183221A CN111183221A (zh) 2020-05-19
CN111183221B true CN111183221B (zh) 2024-04-09

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CN201880050251.7A Active CN111183221B (zh) 2017-05-26 2018-05-25 用于o-糖蛋白的蛋白酶和结合多肽
CN201880050240.9A Active CN111183220B (zh) 2017-05-26 2018-05-25 用于聚糖分析的工具

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CN201880050240.9A Active CN111183220B (zh) 2017-05-26 2018-05-25 用于聚糖分析的工具

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US (3) US12448614B2 (enExample)
EP (2) EP3630967B8 (enExample)
JP (3) JP7195008B2 (enExample)
KR (2) KR102608876B1 (enExample)
CN (2) CN111183221B (enExample)
AU (2) AU2018272984B2 (enExample)
CA (2) CA3063815A1 (enExample)
DK (2) DK3630968T3 (enExample)
ES (2) ES2986557T3 (enExample)
FI (2) FI3630968T3 (enExample)
IL (2) IL270950B2 (enExample)
PL (2) PL3630967T3 (enExample)
PT (2) PT3630968T (enExample)
WO (2) WO2018215657A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111183221B (zh) * 2017-05-26 2024-04-09 杰诺维斯公司 用于o-糖蛋白的蛋白酶和结合多肽
KR20220032576A (ko) * 2019-07-03 2022-03-15 팔레온 파마슈티칼스 인크. 재조합 인간 시알리다제, 시알리다제 융합 단백질, 및 그를 사용하는 방법
CN112795583A (zh) * 2020-11-16 2021-05-14 上海大学 重组唾液酸外切酶的制备方法、表达基因、重组表达载体及构建方法
EP4448782A1 (en) * 2021-12-15 2024-10-23 Danisco US Inc. Compositions and methods involving proteases specific for mannose-modified proteins
WO2024118481A1 (en) * 2022-11-28 2024-06-06 Amgen Inc. Method of processing etanercept

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6720165B2 (en) 2001-06-14 2004-04-13 Zyomix, Inc. Methods for making antibody fragments and compositions resulting therefrom
US7807174B2 (en) 2002-11-22 2010-10-05 Nexbio, Inc. Class of therapeutic protein based molecules
GB0624874D0 (en) 2006-12-13 2007-01-24 Hansa Medical Ab Treatment
WO2009129086A2 (en) 2008-04-18 2009-10-22 New England Biolabs, Inc. Endoglycosidases that cleave o-linked glycans
EP2166085A1 (en) 2008-07-16 2010-03-24 Suomen Punainen Risti Veripalvelu Divalent modified cells
US8603792B2 (en) 2009-03-27 2013-12-10 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
GB201115841D0 (en) 2011-09-13 2011-10-26 Genovis Ab Protein and method
GB201316744D0 (en) 2013-09-20 2013-11-06 Genovis Ab Method
WO2016003795A1 (en) 2014-07-02 2016-01-07 New England Biolabs, Inc. Universal n-glycan binding reagent
WO2017052433A1 (en) 2015-09-24 2017-03-30 Saab Ab A modular fluid actuator system
JP7123801B2 (ja) 2016-02-04 2022-08-23 ジェノビス エービー 新しい連鎖球菌プロテアーゼ
CN111183221B (zh) * 2017-05-26 2024-04-09 杰诺维斯公司 用于o-糖蛋白的蛋白酶和结合多肽

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
"hypothetical protein Amuc_1119 [Akkermansia muciniphila ATCC BAA-835]，ACD04945.1;Lucas,S. et al.;《GenBank》;第1-2页 *
Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila;Kun Huang et al.;《Carbohydrate Research》;第415卷;第60-65页 *
Evolutionary Families of Metallopeptidases;NElL D. RAWLINGS et al.;《METHODS IN ENZYMOLOGY》;第248卷;第183页摘要、第184页第3段 *
Identification of Glutamate Residues Essential for Catalytic Activity and Zinc Coordination in Aminopeptidase A;Gilles Vazeux et al.;《THE JOURNAL OF BIOLOGICAL CHEMISTRY》;第271卷(第15期);第906页左栏第1段 *
Lucas,S. et al.."hypothetical protein Amuc_1119 [Akkermansia muciniphila ATCC BAA-835]，ACD04945.1.《GenBank》.2013,第1-2页. *
细菌来源金属蛋白酶的研究进展;杭锋等;《工业微生物》;第45卷(第45卷);第53-59页 *

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Publication number Publication date
EP3630968B1 (en) 2024-05-29
AU2018272984B2 (en) 2024-05-23
JP2023103233A (ja) 2023-07-26
JP7195008B2 (ja) 2022-12-23
KR20200011955A (ko) 2020-02-04
EP3630968B8 (en) 2024-07-03
WO2018215656A1 (en) 2018-11-29
IL270950B2 (en) 2025-06-01
PL3630968T3 (pl) 2024-10-07
WO2018215657A9 (en) 2019-03-14
EP3630967B1 (en) 2024-06-12
EP3630967B8 (en) 2024-07-17
US20200087643A1 (en) 2020-03-19
PL3630967T3 (pl) 2024-11-18
DK3630968T3 (da) 2024-07-29
CN111183221A (zh) 2020-05-19
JP2020521460A (ja) 2020-07-27
PT3630968T (pt) 2024-07-30
WO2018215656A9 (en) 2019-03-14
EP3630967A1 (en) 2020-04-08
CN111183220A (zh) 2020-05-19
WO2018215657A1 (en) 2018-11-29
KR102609790B1 (ko) 2023-12-04
PT3630967T (pt) 2024-08-21
JP2020522242A (ja) 2020-07-30
US20200172889A1 (en) 2020-06-04
KR20200010322A (ko) 2020-01-30
US12448614B2 (en) 2025-10-21
ES2986910T3 (es) 2024-11-13
KR102608876B1 (ko) 2023-11-30
IL270950B1 (en) 2025-02-01
IL270949A (en) 2020-01-30
AU2018272400B2 (en) 2024-06-13
US11584922B2 (en) 2023-02-21
CA3063815A1 (en) 2018-11-29
JP7557896B2 (ja) 2024-09-30
AU2018272400A1 (en) 2019-12-05
IL270950A (en) 2020-01-30
EP3630968A1 (en) 2020-04-08
FI3630967T3 (fi) 2024-08-12
CN111183220B (zh) 2024-03-22
CA3063828A1 (en) 2018-11-29
IL270949B1 (en) 2025-02-01
AU2018272984A1 (en) 2019-12-05
IL270949B2 (en) 2025-06-01
US20230212543A1 (en) 2023-07-06
ES2986557T3 (es) 2024-11-11
FI3630968T3 (fi) 2024-07-24
DK3630967T3 (da) 2024-08-19

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