CN111166791A - 一种橄榄叶提取物制备方法 - Google Patents

一种橄榄叶提取物制备方法 Download PDF

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CN111166791A
CN111166791A CN201811346256.2A CN201811346256A CN111166791A CN 111166791 A CN111166791 A CN 111166791A CN 201811346256 A CN201811346256 A CN 201811346256A CN 111166791 A CN111166791 A CN 111166791A
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leaf extract
olive leaf
ethanol
preparation
extracting
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王俊
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Nanjing Mengyuan Kangde Biotechnology Co Ltd
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Nanjing Mengyuan Kangde Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
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Abstract

本发明公开了一种橄榄叶提取物制备方法及应用，所述橄榄叶提取物制备方法包括：a、将橄榄叶用乙醇水溶液提取，取滤液备用；b、将所得药渣加乙醇重复提取；c、合并滤液，于恒温水浴蒸干得橄榄叶提取物。本发明所述橄榄叶提取物中主要含有裂环烯醚萜及其苷、黄酮及其苷、双黄酮及其苷、低分子单宁等成分，裂环烯醚萜类为主要活性成分。具有广谱抗菌作用和消炎抗氧化作用。

Description

一种橄榄叶提取物制备方法
技术领域
本发明涉及生物提取技术领域，特别涉及一种橄榄叶提取物制备方法。
背景技术
油橄榄是木犀科木犀榄属常绿乔木，是世界著名的木本油料兼果用树种，栽培品种有较高食用价值，含丰富优质食用植物油——油橄榄油，为著名亚热带果树和重要经济林木，主要分布于地中海国家，希腊、意大利、突尼斯、西班牙为集中产地。橄榄叶提取物是内服的广谱抗菌成分。目前为止在橄榄叶中鉴定出最具活性的物质是洋橄榄苦甙，一类被归于裂环烯醚萜类的苦味单帖类皂甙。洋橄榄苦甙和其水解产物对橄榄叶的抗菌功能而言，具有独特的重要意义。在欧美等国家，橄榄叶提取物主要做为膳食补充剂，用于调节免疫力。橄榄叶提取物具有以下功能：（1）广谱抗菌作用（2）抗氧化作用：橄榄苦甙可以保护皮肤细胞不受紫外线的伤害（3）加强免疫系统（4）预防心血管疾病。
发明内容
基于以上目的，本发明提供一种橄榄叶提取物制备方法。
本发明涉及一种橄榄叶提取物，所其制备方法包括：
a、橄榄叶1.5 kg，加 3 倍体积 95% 乙醇超声波提 取 30 min，取滤液备用；
b、将所得药渣加 95% 乙醇重复提取 2 次；
c、合并3次滤液，于60℃恒温水浴蒸干，得橄榄叶乙醇提取物288g，提取率为19.2%。
具体实施方式
以下将结合实施例对本发明做进一步说明。
实施例1
橄榄叶1.5 kg，加 3 倍体积 95% 乙醇超声波提取30 min，取滤液，药渣加 95% 乙醇重复提取 2 次，合并3次滤液，于 60℃ 恒温水浴蒸干，得橄榄叶乙 醇提取物 288 g，提取率为 19.2% 。
实施例2
橄榄叶1.5 kg，加 3 倍体积 95% 乙醇超声波提取40min，取滤液，药渣加 95% 乙醇重复提取3次，合并3次滤液，于60℃恒温水浴蒸干，得橄榄叶乙醇提取物 294 g，提取率为19.6% 。

Claims (4)

1.一种橄榄叶提取物制备方法，其特征在于，所述橄榄叶提取物制备方法包括：a、将橄榄叶用乙醇水溶液提取，取滤液备用；b、将所得药渣加乙醇重复提取；c、合并滤液，于恒温水浴蒸干得橄榄叶提取物。
2.根据权利要求1所述的步骤a中乙醇水溶液浓度为95%，用量为橄榄叶3倍体积量,超声波提取30min。
3.根据权利要求1所述的步骤b中乙醇水溶液浓度为95%，重复提取2次。
4.根据权利要求1所述的步骤c中合并3次滤液，于60℃恒温水水浴蒸干。
CN201811346256.2A 2018-11-13 2018-11-13 一种橄榄叶提取物制备方法 Pending CN111166791A (zh)

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