CN111154820A - 一种降低核酸扩增复制滑移率的方法 - Google Patents
一种降低核酸扩增复制滑移率的方法 Download PDFInfo
- Publication number
- CN111154820A CN111154820A CN202010030832.3A CN202010030832A CN111154820A CN 111154820 A CN111154820 A CN 111154820A CN 202010030832 A CN202010030832 A CN 202010030832A CN 111154820 A CN111154820 A CN 111154820A
- Authority
- CN
- China
- Prior art keywords
- amplification
- nucleic acid
- replication
- slip
- pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 138
- 230000003321 amplification Effects 0.000 title claims abstract description 137
- 230000010076 replication Effects 0.000 title claims abstract description 78
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 75
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 71
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 71
- 238000000034 method Methods 0.000 title claims abstract description 58
- 238000012408 PCR amplification Methods 0.000 claims abstract description 16
- 239000012634 fragment Substances 0.000 claims description 35
- 230000003252 repetitive effect Effects 0.000 claims description 27
- 238000011144 upstream manufacturing Methods 0.000 claims description 17
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims description 11
- 229910001425 magnesium ion Inorganic materials 0.000 claims description 11
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 9
- 239000012736 aqueous medium Substances 0.000 claims description 6
- 239000007853 buffer solution Substances 0.000 claims description 6
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 5
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims description 5
- 229960003237 betaine Drugs 0.000 claims description 5
- YCUVUDODLRLVIC-UHFFFAOYSA-N Sudan black B Chemical compound C1=CC(=C23)NC(C)(C)NC2=CC=CC3=C1N=NC(C1=CC=CC=C11)=CC=C1N=NC1=CC=CC=C1 YCUVUDODLRLVIC-UHFFFAOYSA-N 0.000 claims description 3
- 239000003623 enhancer Substances 0.000 claims description 2
- 239000011324 bead Substances 0.000 description 75
- 239000000047 product Substances 0.000 description 61
- 108020004414 DNA Proteins 0.000 description 45
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 40
- 239000000203 mixture Substances 0.000 description 33
- 239000006228 supernatant Substances 0.000 description 28
- 239000000243 solution Substances 0.000 description 25
- 102000004190 Enzymes Human genes 0.000 description 20
- 108090000790 Enzymes Proteins 0.000 description 20
- 230000000052 comparative effect Effects 0.000 description 15
- 238000001514 detection method Methods 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 11
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- 239000012149 elution buffer Substances 0.000 description 7
- 238000010009 beating Methods 0.000 description 6
- 238000007664 blowing Methods 0.000 description 6
- 239000006210 lotion Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000011027 product recovery Methods 0.000 description 6
- 230000000717 retained effect Effects 0.000 description 6
- 238000005406 washing Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 102000053602 DNA Human genes 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000012163 sequencing technique Methods 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 4
- 230000037431 insertion Effects 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 238000003149 assay kit Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L magnesium chloride Substances [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 3
- 229910001629 magnesium chloride Inorganic materials 0.000 description 3
- 238000011002 quantification Methods 0.000 description 3
- XMTQQYYKAHVGBJ-UHFFFAOYSA-N 3-(3,4-DICHLOROPHENYL)-1,1-DIMETHYLUREA Chemical compound CN(C)C(=O)NC1=CC=C(Cl)C(Cl)=C1 XMTQQYYKAHVGBJ-UHFFFAOYSA-N 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 239000013024 dilution buffer Substances 0.000 description 2
- 239000012154 double-distilled water Substances 0.000 description 2
- 159000000003 magnesium salts Chemical class 0.000 description 2
- 239000002096 quantum dot Substances 0.000 description 2
- 239000011535 reaction buffer Substances 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- GUAHPAJOXVYFON-ZETCQYMHSA-N (8S)-8-amino-7-oxononanoic acid zwitterion Chemical compound C[C@H](N)C(=O)CCCCCC(O)=O GUAHPAJOXVYFON-ZETCQYMHSA-N 0.000 description 1
- 230000004543 DNA replication Effects 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 208000031448 Genomic Instability Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000001818 capillary gel electrophoresis Methods 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
- SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
- RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
- HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 1
- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
CK组 | 30μL体系 |
Panel mix | 4μL |
DNA(200ng) | xμL |
KAPA2G Fast Multiplex Mix | 15μL |
Nuclease-free water | To30μL |
组分 | 30μL体系 |
PCR barcodexx mix* | 2μL |
KAPA2G Fast Multiplex Mix | 15μL |
Nuclease-free water | To 30μL |
组分 | 30μL体系 |
PCR barcodexx mix* | 2μL |
KAPA2G Fast Multiplex Mix | 15μL |
Nuclease-free water | To 30μL |
组分 | 25μL体系 |
ddH2O | 8.5 |
2×Taq Master Mix(Dye Plus) | 12.5 |
Primer1(10μM) | 1 |
Primer2(10μM) | 1 |
Template DNA(20ng) | 2 |
Total | 25 |
组分 | 25μL体系 |
2×Qhusion PCR buffer | 25μL |
10mM dNTPs | 2μL |
10μΜprimerF | 1μL |
10μΜprimerR | 1μL |
Template DNA(20ng) | 2μL |
Qhusion(1.0U/μL) | 1μL |
Nuclease-free water | 25μL |
组分 | 25μL体系 |
Nuclease-free water | 8.5 |
10μΜ primerF | 1μL |
10μΜ primerR | 1μL |
Template DNA(20ng) | 2μL |
2×Gloria Plus1 standard PCR mix | 12.5μL |
Total | 25μL |
组分 | 25μL体系 |
Nuclease-free water | 8.5 |
10μΜ primerF | 1μL |
10μΜ primerR | 1μL |
Template DNA(20ng) | 2μL |
2×Gloria Plus1GC PCR mix | 12.5μL |
Total | 25μL |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010030832.3A CN111154820B (zh) | 2020-01-13 | 2020-01-13 | 一种降低核酸扩增复制滑移率的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010030832.3A CN111154820B (zh) | 2020-01-13 | 2020-01-13 | 一种降低核酸扩增复制滑移率的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN111154820A true CN111154820A (zh) | 2020-05-15 |
CN111154820B CN111154820B (zh) | 2021-12-21 |
Family
ID=70562532
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010030832.3A Active CN111154820B (zh) | 2020-01-13 | 2020-01-13 | 一种降低核酸扩增复制滑移率的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111154820B (zh) |
Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1104814A2 (en) * | 1999-11-25 | 2001-06-06 | President of Tohoku University | Method for predicting risk of developing chronic pulmonary emphysema |
US20050048530A1 (en) * | 2003-03-25 | 2005-03-03 | Stratagene | DNA polymerase fusions and uses thereof |
EP2403944A2 (en) * | 2009-03-06 | 2012-01-11 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
US20140163900A1 (en) * | 2012-06-02 | 2014-06-12 | Whitehead Institute For Biomedical Research | Analyzing short tandem repeats from high throughput sequencing data for genetic applications |
CN104164478A (zh) * | 2014-03-21 | 2014-11-26 | 中国人民解放军第三军医大学第一附属医院 | 一种基因单碱基变异的cras-pcr检测方法 |
WO2016011280A1 (en) * | 2014-07-16 | 2016-01-21 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
CN105671169A (zh) * | 2016-03-08 | 2016-06-15 | 广西特色作物研究院 | 柑橘黄龙病菌亚洲种检测用引物、试剂盒及检测方法 |
CN106661631A (zh) * | 2014-06-06 | 2017-05-10 | 康奈尔大学 | 使用组合的核酸酶、连接酶、聚合酶和测序反应识别和枚举核酸序列、表达、拷贝或dna甲基化变化的方法 |
CN106701988A (zh) * | 2017-02-10 | 2017-05-24 | 上海荻硕贝肯医学检验所有限公司 | 用于检测短串联重复序列的引物、试剂盒及方法 |
AU2016376478A1 (en) * | 2015-12-22 | 2018-07-05 | Diasorin Italia S.P.A. | A method of fluorescent detection of isothermal loop-mediated amplification (LAMP) of a target nucleic acid, oligonucleotides and kits thereof |
CN108531563A (zh) * | 2018-02-05 | 2018-09-14 | 深圳市尚维高科有限公司 | 多孔微球的用途及裂解液以及裂解液的使用方法 |
CN109706233A (zh) * | 2018-12-28 | 2019-05-03 | 广州精科医学检验所有限公司 | 一种复杂长片段核酸序列的扩增技术 |
-
2020
- 2020-01-13 CN CN202010030832.3A patent/CN111154820B/zh active Active
Patent Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1104814A2 (en) * | 1999-11-25 | 2001-06-06 | President of Tohoku University | Method for predicting risk of developing chronic pulmonary emphysema |
US20050048530A1 (en) * | 2003-03-25 | 2005-03-03 | Stratagene | DNA polymerase fusions and uses thereof |
EP2403944A2 (en) * | 2009-03-06 | 2012-01-11 | Synthetic Genomics, Inc. | Methods for cloning and manipulating genomes |
US20140163900A1 (en) * | 2012-06-02 | 2014-06-12 | Whitehead Institute For Biomedical Research | Analyzing short tandem repeats from high throughput sequencing data for genetic applications |
CN104164478A (zh) * | 2014-03-21 | 2014-11-26 | 中国人民解放军第三军医大学第一附属医院 | 一种基因单碱基变异的cras-pcr检测方法 |
CN106661631A (zh) * | 2014-06-06 | 2017-05-10 | 康奈尔大学 | 使用组合的核酸酶、连接酶、聚合酶和测序反应识别和枚举核酸序列、表达、拷贝或dna甲基化变化的方法 |
WO2016011280A1 (en) * | 2014-07-16 | 2016-01-21 | Tangen Biosciences, Inc. | Isothermal methods for amplifying nucleic acid samples |
AU2016376478A1 (en) * | 2015-12-22 | 2018-07-05 | Diasorin Italia S.P.A. | A method of fluorescent detection of isothermal loop-mediated amplification (LAMP) of a target nucleic acid, oligonucleotides and kits thereof |
CN108431235A (zh) * | 2015-12-22 | 2018-08-21 | 索灵股份公司 | 靶核酸的等温环介导扩增(lamp)的荧光检测的方法,寡核苷酸及其试剂盒 |
CN105671169A (zh) * | 2016-03-08 | 2016-06-15 | 广西特色作物研究院 | 柑橘黄龙病菌亚洲种检测用引物、试剂盒及检测方法 |
CN106701988A (zh) * | 2017-02-10 | 2017-05-24 | 上海荻硕贝肯医学检验所有限公司 | 用于检测短串联重复序列的引物、试剂盒及方法 |
CN108531563A (zh) * | 2018-02-05 | 2018-09-14 | 深圳市尚维高科有限公司 | 多孔微球的用途及裂解液以及裂解液的使用方法 |
CN109706233A (zh) * | 2018-12-28 | 2019-05-03 | 广州精科医学检验所有限公司 | 一种复杂长片段核酸序列的扩增技术 |
Non-Patent Citations (6)
Title |
---|
ENRIQUE VIGUERA等: "Replication slippage involves DNA polymerase pausing and dissociation", 《THE EMBO JOURNAL》 * |
MELISSA CASTILLO-LIZARDO等: "Replication slippage of the thermophilic DNA polymerases B and D from the Euryarchaeota Pyrococcus abyssi", 《FRONTIERS IN MICROBIOLOGY》 * |
娄兵海: "柑橘黄龙病菌亚洲种全基因组测序及遗传多样性研究", 《中国博士学位论文全文数据库农业科技辑》 * |
王阳等: "三核苷酸双链重复序列扩展合成特性及其机理", 《生物化学与生物物理进展》 * |
续薇等: "《医学检验与质量管理》", 31 August 2015, 人民军医出版社 * |
陈绘丽等: "错配核酸识别修复的研究进展", 《化学进展》 * |
Also Published As
Publication number | Publication date |
---|---|
CN111154820B (zh) | 2021-12-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11098357B2 (en) | Compositions and methods for identification of a duplicate sequencing read | |
US11274335B2 (en) | Methods for the epigenetic analysis of DNA, particularly cell-free DNA | |
CN110699426B (zh) | 基因目标区域富集方法及试剂盒 | |
US20080241831A1 (en) | Methods for detecting small RNA species | |
JP6785839B2 (ja) | Dna試料を分析するためのプローブ組及びその使用方法 | |
WO2012040387A1 (en) | Direct capture, amplification and sequencing of target dna using immobilized primers | |
JP2020536525A (ja) | プローブ及びこれをハイスループットシーケンシングに適用するターゲット領域の濃縮方法 | |
AU2016403554A1 (en) | Method for enriching target nucleic acid sequence from nucleic acid sample | |
US20200299764A1 (en) | System and method for transposase-mediated amplicon sequencing | |
CN111154820B (zh) | 一种降低核酸扩增复制滑移率的方法 | |
CN114807300A (zh) | 单引物多重扩增技术在检测片段化稀有特征核酸分子中的应用及试剂盒 | |
CN112301430B (zh) | 一种建库方法及应用 | |
EP3810805A1 (en) | Method for detection and quantification of genetic alterations | |
WO2012083845A1 (zh) | 用于除去测序文库中载体片段的方法及其用途 | |
WO2011101744A2 (en) | Region of interest extraction and normalization methods | |
CN106701949B (zh) | 一种减少扩增偏倚的基因突变检测方法和试剂 | |
US20230105642A1 (en) | Method and compositions for preparing nucleic acid libraries | |
CN111315895A (zh) | 用于产生环状单链dna文库的新型方法 | |
JP2023519979A (ja) | ゲノム内の構造再編成の検出方法 | |
CN114085895B (zh) | 快速检测msi的检测引物及其试剂盒 | |
US20210180125A1 (en) | Method for the detection and quantification of genetic alterations | |
WO2024117970A1 (en) | Method for efficient multiplex detection and quantification of genetic alterations | |
CN109355288B (zh) | 一种目标dna富集的方法和应用 | |
CN116064750A (zh) | 一种用于多重pcr扩增的反应体系及其扩增试剂盒 | |
CN116083550A (zh) | 一种短串联重复序列的检测方法、引物组、试剂盒和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Zhao Wei Inventor after: Tang Jiajie Inventor after: Gao Pengfei Inventor after: Yang Jingmin Inventor after: Li Wentao Inventor before: Zhao Wei Inventor before: Li Wentao Inventor before: Tang Jiajie Inventor before: Yang Jingmin |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method to reduce the slip ratio of nucleic acid amplification Effective date of registration: 20221009 Granted publication date: 20211221 Pledgee: Industrial Bank Co.,Ltd. Shanghai Branch Pledgor: Shanghai Wickham Biomedical Technology Co.,Ltd. Registration number: Y2022310000280 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20231017 Granted publication date: 20211221 Pledgee: Industrial Bank Co.,Ltd. Shanghai Branch Pledgor: Shanghai Wickham Biomedical Technology Co.,Ltd.|SHANDONG MAITIAN BIOLOGICAL TECHNOLOGY CO.,LTD. Registration number: Y2022310000280 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A method for reducing the slip rate of nucleic acid amplification replication Effective date of registration: 20231030 Granted publication date: 20211221 Pledgee: Industrial Bank Co.,Ltd. Shanghai Jinshan Branch Pledgor: Shanghai Wickham Biomedical Technology Co.,Ltd.|SHANDONG MAITIAN BIOLOGICAL TECHNOLOGY CO.,LTD. Registration number: Y2023980062901 |