CN111149874A - A weight reducing oil containing tea leaf extract - Google Patents
A weight reducing oil containing tea leaf extract Download PDFInfo
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- CN111149874A CN111149874A CN202010032585.0A CN202010032585A CN111149874A CN 111149874 A CN111149874 A CN 111149874A CN 202010032585 A CN202010032585 A CN 202010032585A CN 111149874 A CN111149874 A CN 111149874A
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- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
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Abstract
The invention relates to the field of fat-reducing oil, in particular to fat-reducing oil containing theanine, which comprises the following substances in parts by weight: 0.1-0.8 wt% of theanine, 0.5-1.5 wt% of polypeptide, 2-10 wt% of camellia oil, 1-3 wt% of perilla seed oil, 0.02-0.05 wt% of lotus leaf extract, 0.3-0.8 wt% of taurine, 0.02-0.06 wt% of dried orange peel volatile oil and the balance of olive oil. The weight-reducing oil provided by the invention can continuously decompose and burn fat and inhibit fat absorption, promotes digestion of food in vivo, has the effects of reducing blood sugar, blood fat and blood pressure and strengthening immunity, and has the advantages of proper taste and no side effect.
Description
Technical Field
The invention relates to the field of fat-reducing oil, and in particular relates to fat-reducing oil containing theanine.
Background
Obesity is a major health challenge in china. The obesity of China is frequent, according to the latest statistics, the overweight and obese population of China reaches 3.25 hundred million people, and 1 obese person exists in every 4 nations.
The World Health Organization (WHO) has defined obesity as a disease in 1997. Summarizing the recent research efforts in the physiological and medical community, obesity-related complications are as follows: hyperlipidemia, hypertension, cardiovascular diseases, respiratory disorders, diabetes, arthropathy, fatty liver, cholelithiasis, pancreatitis, endocrine disorder, infertility, cancer, and mental disorder.
At present, diet and exercise are adopted as fat-reducing methods which are widely applied, and low-calorie food is adopted to replace staple food and diet fat-reducing medicines are taken, but the fat-reducing methods are limited and even have danger; for example: 1) simple diet and exercise are difficult to insist, and diet and exercise are the most healthy and effective weight losing method which is generally accepted all over the world at present, but the biggest problem of the method is difficult to insist. 2) The low calorie food is hard to be adhered to, and the low calorie food refers to various weight-reducing staple food with cellulose or alginate polysaccharide and konjac polysaccharide as main ingredients, including purified cellulose, and natural cellulose food such as wheat bran, whole wheat flour, oatmeal, bean dregs, etc. After a person eats the low-calorie food, because cellulose is in intestines and stomach, the feeling of satiety is strong, but the feeling of satiety is not equal to the energy supply of the body, and the person can lose weight in this way and feel the body is not full, and even feel dizzy. 3) The medicine has limitation on weight reduction, and most of the existing health care products for weight reduction are 'Sanwu' products produced by irregular enterprises. The components contained in the composition can be roughly divided into four types which have serious harm: contains psychotropic drugs, purgative drugs, hormones, and stimulants.
In summary, the obesity problem is serious, and the national health is seriously affected. However, at present, no healthy and easy weight-losing method exists, and even after the weight-losing is successful, the weight-losing is easy to rebound. Therefore, weight loss is a worldwide problem.
Disclosure of Invention
The invention aims to overcome the defects of the prior art, and provides the weight-losing oil containing theanine, which has the functions of losing weight and losing weight, is green and safe, and has no toxic or side effect.
In order to achieve the purpose, the invention provides a tea-green-containing fat-reducing oil which comprises the following substances in parts by weight: 0.1-0.8 wt% of theanine, 0.5-1.5 wt% of polypeptide, 2-10 wt% of camellia oil, 1-3 wt% of perilla seed oil, 0.02-0.05 wt% of lotus leaf extract, 0.3-0.8 wt% of taurine, 0.02-0.06 wt% of dried orange peel volatile oil and the balance of olive oil.
Theaflavin is green tea extract, and can regulate blood lipid, especially reduce content of serum TG (triglyceride), TC (total cholesterol) and low density lipoprotein cholesterol (LDL-C), and increase high density lipoprotein; the folium Nelumbinis extract contains various alkaloids (such as nuciferine, dehydronuciferine, nelumbine, dehydronuciferine, mexicanin, Veronicine, O-demethylnuciferine, N-demethylnuciferine), flavonoids (quercetin, luteolin, 3-methoxyquercetin) and polyphenols (catechin, epicatechin, Nympholide A2 and Nympholide B3). The pericarpium Citri Tangerinae volatile oil contains d-limonene, citral, nobiletin, hesperidin, inositol, vitamin Bx, etc. According to the invention, the theanine, the lotus leaf extract, the dried orange peel volatile oil and the camellia oil are compounded and then added into the olive oil, so that the weight-losing and lipid-lowering effects of the olive oil can be obviously improved, and the health-care effect of the olive oil is improved.
Under the preferable conditions, the tea-green-containing fat-reducing oil comprises the following substances in parts by weight: 0.2-0.6 wt% of theanine, 0.8-1.2 wt% of polypeptide, 3-6 wt% of camellia oil, 1.5-2.6 wt% of perilla seed oil, 0.03-0.04 wt% of lotus leaf extract, 0.4-0.6 wt% of taurine, 0.03-0.05 wt% of dried orange peel volatile oil and the balance of olive oil.
Under the preferable conditions, the tea-green-containing fat-reducing oil consists of the following substances in parts by weight: 0.38 wt% of theanine, 0.92 wt% of polypeptide, 3.28 wt% of camellia oil, 1.85 wt% of perilla seed oil, 0.03 wt% of lotus leaf extract, 0.52 wt% of taurine, 0.04 wt% of dried orange peel volatile oil and the balance of olive oil.
Preferably, the polypeptide is at least one selected from the group consisting of a deep sea fish oligopeptide, a soybean peptide and a neuroactive peptide.
The neuroactive peptide has lasting effect and no toxic or side effect, can enable an organism to be in an excited state, continuously consume energy and achieve the effect of losing weight and reducing fat, and is selected from at least one of opioid active peptide, endorphin and enkephalin under the optimal condition.
In order to make the most of the beneficial components in the lotus leaf utilized, the preparation method of the lotus leaf extract comprises the following steps under the preferable conditions:
1) crushing the dried lotus leaves, sieving the crushed lotus leaves with a 80-mesh sieve, and extracting the lotus leaves for 2-3 times by using ethanol as a solvent and adopting an ultrasonic-assisted heating method to obtain an extracting solution 1;
2) sequentially extracting the extracting solution 1 with petroleum ether, chloroform, ethyl acetate and n-butanol, and concentrating and purifying the obtained chloroform part mixed solution, ethyl acetate part mixed solution and n-butanol part mixed solution.
Preferably, in the step 1), the ratio of the lotus leaves to the solvent is 1 Kg: 10-40L; the ultrasonic power is 300-400W.
Under the preferable conditions, the extraction temperature of the ultrasonic auxiliary heating method is 75-90 ℃, and the extraction time is 1-1.5 h.
Preferably, in the step (2), the method for concentrating and purifying the chloroform fraction mixture comprises: filtering part of the mixed solution of chloroform by using an ultrafiltration membrane, and then carrying out spray drying.
Preferably, in the step (2), the method for concentrating and purifying the ethyl acetate partial mixed solution comprises the following steps: distilling the ethyl acetate part of the mixed solution under reduced pressure until the ethyl acetate is completely evaporated, dissolving the fraction with water, passing the obtained water solution through macroporous adsorption resin, sequentially eluting with water and 50-75% ethanol until the water solution is colorless, collecting ethanol eluate, concentrating and drying.
Preferably, in the step (2), the method for concentrating and purifying the n-butanol fraction mixed solution comprises the following steps: distilling the n-butanol part of the mixed solution under reduced pressure until the n-butanol is completely evaporated, dissolving the fraction with water, subjecting the obtained water solution to polyamide column chromatography, performing gradient elution with ethanol-water, each gradient elution being 3-5 times the column volume, and collecting 30-50% ethanol eluate.
In a preferred embodiment of the present invention, the method for extracting the lotus leaf extract comprises:
1) crushing dried lotus leaves, sieving the crushed lotus leaves with a 80-mesh sieve, extracting the dried lotus leaves for 2-3 times by using an ultrasonic-assisted heating method at the extraction temperature of 60-85 ℃ for 0.5-2 hours each time by using ethanol as a solvent, combining the extracting solutions, and removing filter residues from the extracting solutions through vacuum filtration to obtain an extracting solution 1;
2) sequentially extracting the extracting solution 1 with petroleum ether, chloroform, ethyl acetate and n-butanol, and further separating the obtained chloroform part, ethyl acetate part and n-butanol part;
filtering part of the mixed liquor of the chloroform by adopting an ultrafiltration membrane, and then carrying out spray drying;
4) concentrating the ethyl acetate part obtained in the step 2) under reduced pressure to dryness, dissolving with distilled water, loading the obtained water solution on macroporous adsorption resin, eluting with deionized water with 10 times of column volume, sequentially eluting with water and 50-75% ethanol, respectively washing to colorless, collecting ethanol eluate, concentrating, and drying to obtain folium Nelumbinis total flavone, wherein the macroporous adsorption resin is AB-8 macroporous resin, the sample loading concentration is 20mg/mL, the sample loading volume is 20mL, the diameter-height ratio is 1:30, and the ethanol concentration is 70%;
5) concentrating the n-butanol part obtained in the step 2) under reduced pressure to dryness, dissolving with distilled water, subjecting the obtained water solution to polyamide column chromatography, performing gradient elution with ethanol-water, each gradient elution being 3-5 times the column volume, collecting 30-50% ethanol eluate, concentrating and drying to obtain the total polyphenol of lotus leaves.
Under the preferable conditions, the extraction method of the theanine comprises the following steps:
1) crushing tea leaves to 5-20 meshes, mixing the crushed tea leaves with purified water, wherein the using amount of the purified water is 10-40% of the mass of the tea leaves, and placing the mixture containing the tea leaves and the purified water in an environment of 110-140 ℃ for ultrasonic wave auxiliary heating treatment for 3-8 min to obtain a mixture A;
2) adding 10-40% of ethanol into the mixture A, and carrying out smouldering treatment on the mixture A after the ethanol is added for 5-10 min to fully mix the tea powder and the ethanol to obtain a mixture B;
3) and (3) performing supercritical extraction, namely putting the mixture B into a supercritical carbon dioxide extraction kettle, and treating for 30-60 min at 50-60 ℃ and under the pressure of 30-50 MPa to obtain the theanine.
Through the technical scheme, the invention has the following technical effects:
the weight-reducing oil provided by the invention can continuously decompose and burn fat and inhibit fat absorption, promotes digestion of food in vivo, has the effects of reducing blood sugar, blood fat and blood pressure and strengthening immunity, and has the advantages of proper taste and no side effect.
Detailed Description
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
In the following examples, the extraction method of the lotus leaf extract is as follows:
1) crushing dried lotus leaves, sieving the crushed lotus leaves with a 80-mesh sieve, extracting for 3 times by using an ultrasonic-assisted heating method at the extraction temperature of 75 ℃ for 1.2 hours each time by using ethanol as a solvent, combining extracting solutions, and removing filter residues from the extracting solutions through vacuum filtration to obtain an extracting solution 1;
2) sequentially extracting the extracting solution 1 with petroleum ether, chloroform, ethyl acetate and n-butanol, and further separating the obtained chloroform part, ethyl acetate part and n-butanol part;
filtering part of the mixed liquor of the chloroform by adopting an ultrafiltration membrane, and then carrying out spray drying to obtain a product A;
3) concentrating the ethyl acetate part obtained in the step 2) under reduced pressure until the ethyl acetate part is dried, dissolving the ethyl acetate part with distilled water, loading the obtained water solution onto macroporous adsorption resin, eluting the macroporous adsorption resin with deionized water of which the volume is 10 times of that of a column, sequentially eluting the water and 50-75% ethanol until the ethanol is colorless, collecting ethanol eluent, concentrating and drying the ethanol eluent to obtain a product B, wherein the macroporous adsorption resin is AB-8 macroporous resin, the loading concentration is 20mg/mL, the loading volume is 20mL, the diameter-height ratio is 1:30, and the ethanol concentration is 70%;
5) concentrating the n-butanol fraction obtained in step 2) under reduced pressure to dryness, dissolving with distilled water, subjecting the obtained water solution to polyamide column chromatography, performing gradient elution with ethanol-water, each gradient elution being 3-5 times the column volume, collecting 30-50% ethanol eluate, concentrating, and drying to obtain product C
6) And mixing the product A, the product B and the product C to obtain the lotus leaf extract.
In the following examples, the extraction method of theanine is as follows:
1) crushing tea leaves to 20 meshes, mixing the crushed tea leaves with purified water, wherein the using amount of the purified water is 30% of the mass of the tea leaves, and placing the mixture containing the tea leaves and the purified water in an environment of 120 ℃ for 5min under ultrasonic wave auxiliary heating treatment to obtain a mixture A;
2) adding 25% ethanol into the mixture A, and moistening the mixture A after adding ethanol for 8min to fully mix the tea powder and ethanol to obtain a mixture B;
3) performing supercritical extraction, placing the mixture B into a supercritical carbon dioxide extraction kettle, and treating at 60 deg.C and 50MPa for 45min to obtain theanine.
Example 1
A weight-losing oil containing theanine is composed of the following substances in parts by weight: 0.38 wt% of theanine, 0.2 wt% of endorphin, 0.4 wt% of enkephalin, 0.32 wt% of soybean peptide, 3.28 wt% of camellia oil, 1.85 wt% of perilla seed oil, 0.03 wt% of lotus leaf extract, 0.52 wt% of taurine, 0.04 wt% of dried orange peel volatile oil and the balance of olive oil.
Example 2
A weight-losing oil containing theanine is composed of the following substances in parts by weight: 0.6 wt% of theanine, 0.3 wt% of endorphin, 0.5 wt% of deep sea fish oligopeptide, 6 wt% of camellia oil, 1.5 wt% of perilla seed oil, 0.04 wt% of lotus leaf extract, 0.4 wt% of taurine, 0.05 wt% of dried orange peel volatile oil and the balance of olive oil.
Example 3
A weight-losing oil containing theanine is composed of the following substances in parts by weight: 0.2 wt% of theanine, 1 wt% of enkephalin, 0.2 wt% of opioid active peptide, 3 wt% of camellia oil, 2.6 wt% of perilla seed oil, 0.03 wt% of lotus leaf extract, 0.6 wt% of taurine, 0.03 wt% of dried orange peel volatile oil and the balance of olive oil.
Example 4
A weight-losing oil containing theanine is composed of the following substances in parts by weight: 0.8 wt% of theanine, 1.5 wt% of deep sea fish oligopeptide, 2 wt% of camellia oil, 3 wt% of perilla seed oil, 0.02 wt% of lotus leaf extract, 0.8 wt% of taurine, 0.02 wt% of dried orange peel volatile oil and the balance of olive oil.
Example 5
A weight-losing oil containing theanine is composed of the following substances in parts by weight: 0.1 wt% of theanine, 0.5 wt% of soybean peptide, 10 wt% of camellia oil, 1 wt% of perilla seed oil, 0.05 wt% of lotus leaf extract, 0.3 wt% of taurine, 0.06 wt% of dried orange peel volatile oil and the balance of olive oil.
Comparative example 1
The procedure of example 1 was followed except that theaflavin was not included.
Comparative example 2
The procedure of example 1 was followed except that tea leaf extract was not contained.
Comparative example 3
The method of example 1 was followed except that the dried orange peel volatile oil was not included.
Examples of the experiments
(1) Experimental animals and environment: 120 SPF SD male white rats come from the experimental animal center of Guangxi medical university and weigh 220-250 g. Animal room temperature: 22-25 ℃, relative humidity: 55-75%, and respectively administering high calorie feedstuff as high fat feedstuff group. The food intake, food amount scattered, and food remaining amount were recorded every week, and the body weight was weighed 1 time. After feeding for 2 weeks, rats in the high-fat diet group were ranked according to weight gain, and obesity-resistant rats with lower weight gain were eliminated. The screened 80 obesity-sensitive rats were randomly divided into 8 groups by weight, weighed m1, and then fed with general feeds containing the fat-reducing oils of examples 1 to 5 and controls 1 to 3 (the addition amount of the fat-reducing oil was 10%), continuously fed for one month, after the test was completed, weighed m2, and 1% sodium pentobarbital (0.5mL/100gBW) for anesthesia, the fat around the kidney was dissected and taken, and the fat/body weight ratio was calculated, and the test results are shown in Table 1.
Table 1:
| m1(g) | m2(g) | fat/body weight ratio (%) | |
| Example 1 | 428.8 | 205.5 | 4.52 |
| Example 2 | 421.3 | 228.3 | 4.75 |
| Example 3 | 427.8 | 236.4 | 4.82 |
| Example 4 | 433.8 | 215.3 | 4.59 |
| Example 5 | 441.5 | 229.6 | 4.60 |
| Comparative example 1 | 436.7 | 333.8 | 5.23 |
| Comparative example 2 | 425.1 | 305.2 | 5.08 |
| Comparative example 3 | 427.5 | 287.5 | 4.87 |
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.
Claims (10)
1. The weight-losing oil containing theanine is characterized by comprising the following substances in parts by weight: 0.1-0.8 wt% of theanine, 0.5-1.5 wt% of polypeptide, 2-10 wt% of camellia oil, 1-3 wt% of perilla seed oil, 0.02-0.05 wt% of lotus leaf extract, 0.3-0.8 wt% of taurine, 0.02-0.06 wt% of dried orange peel volatile oil and the balance of olive oil.
2. The tea-green-containing fat-reducing oil as claimed in claim 1, which comprises the following substances in weight percentage: 0.2-0.6 wt% of theanine, 0.8-1.2 wt% of polypeptide, 3-6 wt% of camellia oil, 1.5-2.6 wt% of perilla seed oil, 0.03-0.04 wt% of lotus leaf extract, 0.4-0.6 wt% of taurine, 0.03-0.05 wt% of dried orange peel volatile oil and the balance of olive oil.
3. The tea-green-containing fat-reducing oil as claimed in claim 2, which is characterized by comprising the following components in percentage by weight: 0.38 wt% of theanine, 0.92 wt% of polypeptide, 3.28 wt% of camellia oil, 1.85 wt% of perilla seed oil, 0.03 wt% of lotus leaf extract, 0.52 wt% of taurine, 0.04 wt% of dried orange peel volatile oil and the balance of olive oil.
4. The tea-leaf-extract-containing fat-reducing oil as claimed in claim 1, wherein the polypeptide is at least one selected from the group consisting of deep sea fish oligopeptide, soybean peptide and neuroactive peptide.
5. The tea-cyanine-containing fat-reducing oil of claim 4, wherein the neuroactive peptides are selected from at least one of opioid active peptides, endorphins, and enkephalins.
6. The tea-leaf-extract-containing fat-reducing oil as claimed in claim 1, wherein the preparation method of the lotus leaf extract comprises the steps of:
1) crushing the dried lotus leaves, sieving the crushed lotus leaves with a 80-mesh sieve, and extracting the lotus leaves for 2-3 times by using ethanol as a solvent and adopting an ultrasonic-assisted heating method to obtain an extracting solution 1;
2) sequentially extracting the extracting solution 1 with petroleum ether, chloroform, ethyl acetate and n-butanol, and concentrating and purifying the obtained chloroform part mixed solution, ethyl acetate part mixed solution and n-butanol part mixed solution.
7. The tea-leaf-containing fat-reducing oil as claimed in claim 6, wherein in the step 1), the ratio of the lotus leaves to the solvent is 1 Kg: 10-40L; the ultrasonic power is 300-400W;
the extraction temperature of the ultrasonic auxiliary heating method is 75-90 ℃, and the extraction time is 1-1.5 h.
8. The tea-leaf-extract-containing fat-reducing oil as claimed in claim 6, wherein in the step (2), the method for concentrating and purifying the chloroform fraction mixture comprises: filtering part of the mixed solution of chloroform by using an ultrafiltration membrane, and then carrying out spray drying.
9. The tea-leaf-extract-containing fat-reducing oil as claimed in claim 6, wherein in the step (2), the method for concentrating and purifying the ethyl acetate partial mixed solution comprises the following steps: distilling the ethyl acetate part of the mixed solution under reduced pressure until the ethyl acetate is completely evaporated, dissolving the fraction with water, passing the obtained water solution through macroporous adsorption resin, sequentially eluting with water and 50-75% ethanol until the water solution is colorless, collecting ethanol eluate, concentrating and drying.
10. The tea-leaf-extract-containing fat-reducing oil as claimed in claim 6, wherein in the step (2), the method for concentrating and purifying the n-butanol fraction mixed solution comprises: distilling the n-butanol part of the mixed solution under reduced pressure until the n-butanol is completely evaporated, dissolving the fraction with water, subjecting the obtained water solution to polyamide column chromatography, performing gradient elution with ethanol-water, each gradient elution being 3-5 times the column volume, and collecting 30-50% ethanol eluate.
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| CN108991141A (en) * | 2018-08-04 | 2018-12-14 | 望江县振兴植物油厂（普通合伙） | A kind of ready-mixed oil with lipid-reducing function |
| CN110638870A (en) * | 2019-07-17 | 2020-01-03 | 湖北爱莲生物科技有限公司 | Method for co-producing multiple active substances from lotus leaves |
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- 2020-01-11 CN CN202010032585.0A patent/CN111149874A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101366522A (en) * | 2008-10-17 | 2009-02-18 | 罗红宇 | Edible nourishing oil with balanced fatty acid rate |
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Application publication date: 20200515 |