CN111148572B - 跨界面磁性分离 - Google Patents

跨界面磁性分离 Download PDF

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Publication number
CN111148572B
CN111148572B CN201880048221.2A CN201880048221A CN111148572B CN 111148572 B CN111148572 B CN 111148572B CN 201880048221 A CN201880048221 A CN 201880048221A CN 111148572 B CN111148572 B CN 111148572B
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chamber
liquid
pmp
transfer
analyte
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Chinese (zh)
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CN111148572A (zh
Inventor
D·M·凯尔索
A·K·阿加瓦尔
S·M·麦克法尔
T·韦斯特伯格
M·A·布泽尔
J·L·里德
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NORTHWEST UNIVERSITY
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0684—Venting, avoiding backpressure, avoid gas bubbles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B01L2300/041—Connecting closures to device or container
    • B01L2300/042—Caps; Plugs
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0829—Multi-well plates; Microtitration plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B01L2300/166—Suprahydrophobic; Ultraphobic; Lotus-effect
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
  • Compounds Of Iron (AREA)
CN201880048221.2A 2017-06-06 2018-06-06 跨界面磁性分离 Active CN111148572B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202111135982.1A CN114011479B (zh) 2017-06-06 2018-06-06 跨界面磁性分离

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762515876P 2017-06-06 2017-06-06
US62/515,876 2017-06-06
PCT/US2018/036348 WO2018226891A1 (en) 2017-06-06 2018-06-06 Trans-interfacial magnetic separation

Related Child Applications (1)

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CN202111135982.1A Division CN114011479B (zh) 2017-06-06 2018-06-06 跨界面磁性分离

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CN111148572A CN111148572A (zh) 2020-05-12
CN111148572B true CN111148572B (zh) 2022-05-06

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CN202111135982.1A Active CN114011479B (zh) 2017-06-06 2018-06-06 跨界面磁性分离

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US (2) US11673134B2 (enExample)
EP (1) EP3634634A4 (enExample)
JP (1) JP7168230B2 (enExample)
CN (2) CN111148572B (enExample)
AU (2) AU2018281310B2 (enExample)
CA (1) CA3065928C (enExample)
WO (1) WO2018226891A1 (enExample)

Families Citing this family (5)

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Publication number Priority date Publication date Assignee Title
EP3993908B1 (en) * 2019-07-03 2023-11-15 Siemens Healthcare Diagnostics Inc. Rotary platform for cell lysing and purification and method of use
EP4275056A4 (en) 2021-01-29 2024-12-11 Abbott Diagnostics Scarborough, Inc. MAGNETIC PARTICLE AIR TRANSMISSION
CN114107035B (zh) * 2021-11-30 2022-06-03 珠海黑马生物科技有限公司 一种封闭式全自动核酸提取试剂盒
WO2023097922A1 (zh) * 2021-11-30 2023-06-08 珠海黑马生物科技有限公司 一种全封闭全自动核酸提取试剂盒
CN114657049B (zh) * 2022-05-12 2023-06-13 广州国家实验室 一种核酸扩增用卡盒

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2307277A (en) * 1976-03-12 1978-09-14 Technicon Instruments Corportion Magnetic separation in automatic chemical analyser
JPH08511721A (ja) * 1993-06-21 1996-12-10 ラブシュステムス オユ 分離方法
CN1275445A (zh) * 1999-05-31 2000-12-06 中国科学院化工冶金研究所 从流体中分离磁性颗粒的连续式高梯度磁分离方法及装置
WO2007110779A2 (en) * 2006-03-29 2007-10-04 Inverness Medical Switzerland Gmbh Assay device and method
CN101198707A (zh) * 2004-05-12 2008-06-11 内诺斯佩尔公司 基于生物条形码检测靶分析物
CN101529250A (zh) * 2006-08-18 2009-09-09 3M创新有限公司 使用声-机械检测系统检测的方法
CN102027367A (zh) * 2008-02-29 2011-04-20 西北大学 促进生物反应的屏障
CN102127596A (zh) * 2009-12-10 2011-07-20 霍夫曼-拉罗奇有限公司 多孔板和盖
CN102803147A (zh) * 2009-06-05 2012-11-28 尹特根埃克斯有限公司 通用样品准备系统以及在一体化分析系统中的用途
CN105793708A (zh) * 2013-12-04 2016-07-20 瑞士斯保德精密诊断有限公司 检验装置
CN105938135A (zh) * 2008-04-12 2016-09-14 Spd瑞士精确诊断有限公司 包含气泡形成机构的分析装置

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3793445A (en) * 1970-04-29 1974-02-19 Wisconsin Alumni Res Found Reagent for radioimmunoassay
US5110727A (en) * 1987-04-03 1992-05-05 Cardiovascular Diagnostics, Inc. Method for performing coagulation assays accurately, rapidly and simply, using dry chemical reagents and paramagnetic particles
IL100866A (en) * 1991-02-06 1995-10-31 Igen Inc Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets
US5395752A (en) * 1993-03-19 1995-03-07 Ciba Corning Diagnostics Corp. Long emission wavelength chemiluminescent compounds and their use in test assays
US6586193B2 (en) * 1996-04-25 2003-07-01 Genicon Sciences Corporation Analyte assay using particulate labels
EP1021723B1 (en) * 1997-09-22 2008-12-10 Novartis Vaccines and Diagnostics, Inc. Buffers for stabilizing hcv antigens
JP2002502856A (ja) 1998-02-04 2002-01-29 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング 核酸の単離および精製方法
JP5219057B2 (ja) * 1998-07-31 2013-06-26 旭化成株式会社 微生物検出用抗体
CN1185492C (zh) * 1999-03-15 2005-01-19 清华大学 可单点选通式微电磁单元阵列芯片、电磁生物芯片及应用
CA2403278A1 (en) * 2000-03-16 2001-09-20 Subramanian Venkat Shastri Microlaboratory devices and methods
DE10013990C2 (de) * 2000-03-22 2003-12-04 Invitek Biotechnik & Biodesign Polyfunktionelles Trägermaterial für die komplexe Nukleinsäureanalytik
US20030027193A1 (en) * 2001-07-23 2003-02-06 Zhibo Gan Non-isotopic rapid method and kit for measurement of levels of therapeutic compounds
CN100442052C (zh) * 2002-08-15 2008-12-10 陕西西大北美基因股份有限公司 磁性荧光微球及其制备方法和采用该磁性荧光微球进行生物分子检测的方法
AU2003302820A1 (en) * 2002-12-05 2004-06-30 Diadexus, Inc. Compositions, splice variants and methods relating to breast specific genes and proteins
DE10356752A1 (de) * 2003-12-04 2005-06-30 Roche Diagnostics Gmbh Beschichtete Testelemente
EP1621890A1 (en) 2004-07-26 2006-02-01 bioMerieux B.V. Device and method for separating, mixing and concentrating magnetic particles with a fluid and use thereof in purification methods
DE102004040785B4 (de) * 2004-08-23 2006-09-21 Kist-Europe Forschungsgesellschaft Mbh Mikrofluidisches System zur Isolierung biologischer Partikel unter Verwendung der immunomagnetischen Separation
US8883487B2 (en) 2004-12-23 2014-11-11 Abbott Point Of Care Inc. Molecular diagnostics system and methods
DE102005039175A1 (de) * 2005-08-18 2007-02-22 Qiagen Gmbh Vorrichtung und Verfahren zum Abtrennen von magnetischen Partikeln aus einer Flüssigkeit
CN1987464B (zh) * 2006-09-29 2012-03-14 浙江大学 磁捕获技术在NF-κB蛋白分离与检测中的应用方法
CN101251541A (zh) * 2008-04-11 2008-08-27 清华大学 无标记噬菌体展示蛋白质芯片及其制备和检测方法
US8093064B2 (en) * 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
EP2334433B1 (en) 2008-10-06 2012-08-15 Koninklijke Philips Electronics N.V. Microfluidic device
CN102256690B (zh) 2008-12-16 2015-11-25 皇家飞利浦电子股份有限公司 疏水阀
WO2010123594A2 (en) * 2009-01-15 2010-10-28 Children's Medical Center Corporation Device for filtration of fluids there through and accompanying method
CN102470373B (zh) 2009-07-17 2014-11-26 皇家飞利浦电子股份有限公司 用于富集磁性粒子的设备
DE102009035941B8 (de) * 2009-08-03 2017-04-27 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Diagnostiksystem
WO2011026030A1 (en) * 2009-08-31 2011-03-03 Mbio Diagnostics Corporation Integrated sample preparation and analyte detection
EP2338595A1 (en) 2009-12-23 2011-06-29 Atonomics A/S Device, method, and system for the quantitative detection of the presence of multiple target analytes.
US20130157283A1 (en) * 2010-01-19 2013-06-20 President And Fellows Of Harvard College Rapid pathogen diagnostic device and method
US8603416B2 (en) 2010-02-26 2013-12-10 Wisconsin Alumni Research Foundation Device for and method of extracting a fraction from a biological sample
US8728410B2 (en) 2010-02-26 2014-05-20 Wisconsin Alumni Research Foundation Device for and method of extracting a fraction from a biological sample
US9677979B2 (en) * 2010-07-16 2017-06-13 Vanderbilt University Low resource processor using surface tension valves for extracting, concentrating and detecting molecular species
CN103540517B (zh) * 2010-07-23 2017-05-24 贝克曼考尔特公司 处理样本的系统及方法
DE102010041621B4 (de) * 2010-09-29 2016-11-03 Hahn-Schickard-Gesellschaft für angewandte Forschung e.V. Verfahren zum Transport magnetischer Partikel
US9766166B2 (en) * 2013-01-09 2017-09-19 Wisconsin Alumni Research Foundation Device and method incorporating a slideable lid for extracting a targeted fraction from a sample
US8728411B2 (en) 2012-09-05 2014-05-20 Wisconsin Alumni Research Foundation Device for and method of isolating a fraction in a biological sample
US10564077B2 (en) 2012-09-05 2020-02-18 Wisconsin Alumni Research Foundation Device for and method of isolating and analyzing a fraction in a biological sample
US9869675B2 (en) * 2013-03-13 2018-01-16 Vanderbilt University Low resource processor using surface tension valves for extracting, concentrating, and detecting whole cells
US11029310B2 (en) 2013-03-14 2021-06-08 Wisconsin Alumni Research Foundation Device and method for extracting a targeted fraction from a sample
US9952209B2 (en) * 2013-07-01 2018-04-24 The University Of Memphis Research Foundation Iron oxide-gold core-shell nanoparticles and uses thereof
US10040062B2 (en) 2014-01-14 2018-08-07 Wisconsin Alumni Research Foundation Device and method for transferring a target between locations
CA2937975A1 (en) * 2014-01-27 2015-07-30 Brevitest Technologies, Llc Discontinuous fluidic systems for point-of-care analyte measurement
US10357780B2 (en) * 2014-10-27 2019-07-23 President And Fellows Of Harvard College Magnetic capture of a target from a fluid
US9518903B2 (en) 2015-01-13 2016-12-13 Gilson, Inc. Adapter for sliding magnetic particle separation
US10302634B2 (en) * 2015-07-01 2019-05-28 Ancera, Llc Tunable affinity system and method for ferrofluid-based capture assays

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2307277A (en) * 1976-03-12 1978-09-14 Technicon Instruments Corportion Magnetic separation in automatic chemical analyser
JPH08511721A (ja) * 1993-06-21 1996-12-10 ラブシュステムス オユ 分離方法
CN1275445A (zh) * 1999-05-31 2000-12-06 中国科学院化工冶金研究所 从流体中分离磁性颗粒的连续式高梯度磁分离方法及装置
CN101198707A (zh) * 2004-05-12 2008-06-11 内诺斯佩尔公司 基于生物条形码检测靶分析物
WO2007110779A2 (en) * 2006-03-29 2007-10-04 Inverness Medical Switzerland Gmbh Assay device and method
CN101529250A (zh) * 2006-08-18 2009-09-09 3M创新有限公司 使用声-机械检测系统检测的方法
CN102027367A (zh) * 2008-02-29 2011-04-20 西北大学 促进生物反应的屏障
CN105938135A (zh) * 2008-04-12 2016-09-14 Spd瑞士精确诊断有限公司 包含气泡形成机构的分析装置
CN102803147A (zh) * 2009-06-05 2012-11-28 尹特根埃克斯有限公司 通用样品准备系统以及在一体化分析系统中的用途
CN102127596A (zh) * 2009-12-10 2011-07-20 霍夫曼-拉罗奇有限公司 多孔板和盖
CN105793708A (zh) * 2013-12-04 2016-07-20 瑞士斯保德精密诊断有限公司 检验装置

Also Published As

Publication number Publication date
AU2018281310A1 (en) 2020-01-02
WO2018226891A1 (en) 2018-12-13
CA3065928A1 (en) 2018-12-13
US11673134B2 (en) 2023-06-13
JP7168230B2 (ja) 2022-11-09
AU2023237099B2 (en) 2025-10-02
JP2020523570A (ja) 2020-08-06
CN111148572A (zh) 2020-05-12
EP3634634A4 (en) 2021-03-10
EP3634634A1 (en) 2020-04-15
CN114011479A (zh) 2022-02-08
CA3065928C (en) 2025-05-20
AU2023237099A1 (en) 2023-10-12
AU2018281310B2 (en) 2023-06-29
CN114011479B (zh) 2023-05-02
US20210146351A1 (en) 2021-05-20
US20240024865A1 (en) 2024-01-25

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