CN111110393B - Bovine semen extraction method - Google Patents

Bovine semen extraction method Download PDF

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Publication number
CN111110393B
CN111110393B CN201911247274.XA CN201911247274A CN111110393B CN 111110393 B CN111110393 B CN 111110393B CN 201911247274 A CN201911247274 A CN 201911247274A CN 111110393 B CN111110393 B CN 111110393B
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China
Prior art keywords
liquid nitrogen
fixedly arranged
semen
shell
bull
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CN201911247274.XA
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CN111110393A (en
Inventor
崔世豪
袁超
王争光
吴成杨
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Qingdao Longming Embryo Biotechnology Co.,Ltd.
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Zhongnan Bai Grassland Group Co ltd
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Publication of CN111110393A publication Critical patent/CN111110393A/en
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/021Apparatus for collecting seminal fluids; Artificial vaginas
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02Preservation of living parts
    • A01N1/0236Mechanical aspects
    • A01N1/0242Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/022Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The invention discloses a bull semen extraction method, which comprises the following steps of: selecting a robust bull to climb across a cow so as to form a conditioned reflex; preparing before semen collection: preparing about 75% of disinfecting alcohol; preparing a false vagina: a disinfected glass rod is stuck with Vaseline and evenly smeared on one half of the inner tube of the false vagina to increase the lubrication degree; loosening the bull: irradiating the bull by using a fluorescent lamp to enable the bull to relax muscles, and collecting sperms: the bull is allowed to climb up to a Taihe, and the testis of the bull is plugged into a false vagina to finish semen collection, and the semen transportation: and (3) putting the semen into liquid nitrogen transportation equipment and then transporting the semen by using an automobile.

Description

Bovine semen extraction method
Technical Field
The invention belongs to the field of bull semen extraction, and particularly relates to a bull semen extraction method.
Background
When bull semen is taken out and put into liquid nitrogen transport tank in the smart method of bull getting at present and carry out long distance transport, need the artifical liquid nitrogen content of often looking over in the liquid nitrogen transport tank, need in time supply the liquid nitrogen when the liquid nitrogen content in the liquid nitrogen transport tank is less than two-thirds, but artifical periodic detection liquid nitrogen content is more loaded down with trivial details, and the cost of labor is high, and the rate of accuracy that detects is lower moreover.
Disclosure of Invention
The invention provides a method for extracting the semen of a cow, which can automatically detect the shortage of liquid nitrogen in a liquid nitrogen transportation tank and supplement the liquid nitrogen during long-distance transportation in order to overcome the defects of the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme: a method for extracting bovine semen comprises the following steps,
a. and (3) regulating the teaching of bulls: selecting a robust bull to climb across a cow so as to form a conditioned reflex;
b. preparing before semen collection: preparing about 75% of disinfecting alcohol;
c. preparing a false vagina: a disinfected glass rod is stuck with Vaseline and evenly smeared on one half of the inner tube of the false vagina to increase the lubrication degree;
d. loosening the bull: utilize fluorescent lamp to shine the bull and make the bull relax muscle
e. And (3) semen collection: allowing the bull to climb onto a Taihu, and plugging the testis of the bull into a false vagina to finish semen collection;
f. and (3) transporting semen: putting the semen into liquid nitrogen transportation equipment and then transporting the semen by using an automobile;
the liquid nitrogen transportation equipment in the step f comprises liquid nitrogen outer shell, an inner container arranged in the liquid nitrogen outer shell, a plurality of lifting cylinders arranged in the inner container, a damping device arranged on the lower side of the liquid nitrogen outer shell and a liquid nitrogen adding device arranged on the inner container;
the method for transporting the liquid nitrogen by using the liquid nitrogen transportation equipment comprises the following steps: put into liquid nitrogen transportation equipment with the seminal fluid in, carry out the shock attenuation to liquid nitrogen and seminal fluid through damping device in the transportation, add the device through liquid nitrogen and can add the liquid nitrogen automatically when liquid nitrogen is not enough in liquid nitrogen transportation equipment.
The disinfecting alcohol with concentration of about 75% comprises anhydrous alcohol, medical vaseline oil, medical cotton and soap.
When in semen collection, the artificial vagina is held by the right hand to form an angle of 40 degrees with the Taihu, the entrance is inclined downwards, and the penis of the bull is naturally introduced into the artificial vagina by the cooperation of the left hand and the right hand.
The artificial vagina for semen collection is composed of a shell, an inner tube, a funnel and a semen collection tube.
The shock absorption device comprises a telescopic pipe fixedly arranged on the lower end face of a liquid nitrogen shell, a plurality of holes arranged on the outer surface of the telescopic pipe in the circumferential direction, a plurality of telescopic rods movably arranged on the holes respectively, a first telescopic spring fixedly arranged on the inner wall of a liquid nitrogen shell and between the telescopic rods, a telescopic cylindrical block arranged in the telescopic pipe in a vertically movable manner, a second telescopic spring fixedly arranged between the lower end face of the liquid nitrogen shell and the telescopic cylindrical block, a base fixedly arranged at the lower end of the telescopic cylindrical block, a fixed pipe fixedly arranged on the upper surface of the base, and a third telescopic spring fixedly arranged between the bottom of the fixed pipe and the telescopic cylindrical block, wherein one opposite ends of the telescopic rods are all arranged into inclined planes;
the step of utilizing damping device to carry out the absorbing is as follows, with this equipment on the car, when the car goes on the road of jolting, the base will make progress impact force transmit the third expanding spring on, the third expanding spring drives flexible cylinder piece rebound, flexible cylinder piece drives second expanding spring rebound, drives the telescopic link and is close to the inner wall removal of liquid nitrogen shell, reaches through first extension spring and absorbs vibrations.
The liquid nitrogen adding device comprises an air outlet hole arranged on the inner container, a first round pipe fixedly arranged in the air outlet hole, a supporting plate fixedly arranged on the right end face of the first round pipe, a first rotating shaft rotatably arranged on the right end face of the supporting plate, a first fan fixedly arranged on the first rotating shaft, a first chain wheel fixedly arranged on the first rotating shaft, a moving block movably arranged in the first round pipe, a plurality of air vents which are arranged on the inner wall of the first round pipe in the circumferential direction and communicated with the outside, a support rod fixedly arranged on the left end face of the moving block, a push rod fixedly arranged on the left end face of the support rod, a liquid inlet hole arranged on the lower side of the air outlet hole, a second round pipe fixedly arranged in the liquid inlet hole, a rotating hole arranged on the upper side of the inner wall of the second round pipe, a rotating rod rotatably arranged on the rotating hole, a first rotating plate fixedly arranged on the upper end of the rotating rod, and a rotating disc fixedly arranged on the rotating rod and positioned in the second round pipe, A second rotating plate fixedly arranged at the lower end of the rotating rod, a matching hole arranged at the lower side of the liquid inlet hole, a straight rod movably arranged in the matching hole, a supporting plate fixedly arranged at the right end surface of a second round pipe, a second rotating shaft rotatably arranged at the right end surface of the supporting plate, a second fan fixedly arranged at the right end of the second rotating shaft, a second chain wheel fixedly arranged at the outer surface of the second rotating shaft, a transmission chain in transmission connection between the first chain wheel and the second chain wheel, a feeding shell fixedly arranged at the left end surface of the second round pipe, a feeding space arranged in the feeding shell and provided with a left opening, two sliding trapezoidal grooves respectively arranged at the left end surface of the feeding shell, a moving plate vertically movably arranged at the left end surface of the feeding shell, two sliding trapezoidal blocks fixedly arranged at the upper side and the lower side of the right end surface of the moving plate and capable of vertically sliding in the two sliding trapezoidal grooves, and a connecting pipeline fixedly arranged at the left end surface of the moving plate and penetrating through the moving plate, a position sensor is fixedly arranged in the inner part;
the liquid nitrogen adding step by using the liquid nitrogen adding device is as follows; the external liquid nitrogen storage tank is connected and connected through a pipeline thread, the pressure intensity in the inner container can be increased after the liquid nitrogen in the inner container is vaporized, when the pressure intensity reaches a certain degree, the moving block can be pushed to move leftwards, the left side can be driven to move, the ninety degrees of rotation can be driven to reach the left side, the ninety degrees of rotation can be driven at the same time, when the position sensor senses that the position sensor is started after the ninety degrees of rotation is reached, the position sensor drives the water pump to start, the liquid nitrogen in the liquid nitrogen storage tank is input into the inner container by the water pump, the liquid nitrogen can be driven to rotate, the first chain wheel is driven to rotate, the nitrogen can be rapidly discharged through the rotation, the lifting plate can be driven to move downwards through the entering of the liquid nitrogen, when the lifting plate moves downwards, the lifting plate can be driven to move outwards when colliding with the lifting plate, the left side can be driven to rotate ninety degrees from the front side, the rotation can be driven to rotate, the rear side can be driven to rotate from the ninety degrees from the left side, therefore, the sperm box can be pushed to move rightwards in the rotating process, and can be pushed to move rightwards to reset so as to reseal the first circular tube, thereby keeping low temperature in the liquid nitrogen tank and preventing the sperm from being influenced in the transportation process.
In summary, the invention has the following advantages: the method is simple to use, and can automatically add the liquid nitrogen when the content of the liquid nitrogen in the liquid nitrogen transportation equipment is insufficient, so that the labor cost is saved, the accuracy of checking the content of the liquid nitrogen is improved, and the death of sperms in the semen caused by that the liquid nitrogen is not timely added due to manual errors when the content of the liquid nitrogen is insufficient is avoided.
Drawings
FIG. 1 is a top view of the present invention.
Fig. 2 is a perspective view of the present invention.
Fig. 3 is a sectional view taken at a-a in fig. 1.
Fig. 4 is a sectional perspective view at B-B in fig. 1.
Fig. 5 is a perspective view in section at C-C in fig. 1.
Fig. 6 is a cross-sectional view taken at D-D in fig. 1.
Fig. 7 is an enlarged view of fig. 3 at G.
Detailed Description
In order to achieve the purpose, the invention adopts the following technical scheme: a method for extracting bovine semen comprises the following steps,
a. and (3) bull training: selecting a robust bull to climb across a cow so as to form a conditioned reflex;
b. preparing before semen collection: preparing about 75% of disinfecting alcohol;
c. preparation of a false vagina: a disinfected glass rod is stuck with Vaseline and evenly smeared on one half of the inner tube of the false vagina to increase the lubrication degree;
d. loosening the bull: utilize fluorescent lamp to shine the bull and make the bull relax muscle
e. And (3) semen collection: allowing the bull to climb onto a Taihu, and plugging the testis of the bull into a false vagina to finish semen collection;
f. and (3) transporting semen: putting the semen into liquid nitrogen transportation equipment and then transporting the semen by using an automobile;
the disinfecting alcohol with concentration of about 75% comprises anhydrous alcohol, medical vaseline oil, medical cotton and soap.
When in semen collection, the artificial vagina is held by the right hand to form an angle of 40 degrees with the Taihu, the entrance is inclined downwards, and the penis of the bull is naturally introduced into the artificial vagina by the cooperation of the left hand and the right hand.
The artificial vagina for semen collection is composed of a shell, an inner tube, a funnel and a semen collection tube.
As shown in fig. 1-7, the liquid nitrogen transportation device in step f includes a liquid nitrogen housing 1, a rectangular groove disposed on the inner wall of the liquid nitrogen housing 1, an inner container 2 disposed in the liquid nitrogen housing 1, a lifting plate disposed in the inner container 2 in a manner of moving up and down, an adjusting spring fixedly disposed between the lifting plate and the bottom of the inner container 2, a plurality of lifting cylinders 100 disposed on the upper end surface of the lifting plate, a shock absorbing device disposed on the lower side of the liquid nitrogen housing, a liquid nitrogen adding device disposed on the inner container 2, and an anti-tipping device disposed on the lifting cylinders 100.
The shock absorption device comprises a telescopic pipe 3 fixedly arranged on the lower end face of a liquid nitrogen shell, a plurality of holes arranged on the outer surface of the telescopic pipe 3 in the circumferential direction, a plurality of telescopic rods 4 movably arranged on the holes respectively, a first telescopic spring fixedly arranged between the inner wall of the liquid nitrogen shell and the telescopic rods 4, a telescopic cylindrical block 75 arranged in the telescopic pipe 3 in a vertically movable manner, a second telescopic spring fixedly arranged between the lower end face of the liquid nitrogen shell 1 and the telescopic cylindrical block 75, a base fixedly arranged at the lower end of the telescopic cylindrical block 75, a fixed pipe 76 fixedly arranged on the upper surface of the base, and a third telescopic spring fixedly arranged between the bottom of the fixed pipe 76 and the telescopic cylindrical block 75, wherein the opposite ends of the telescopic rods are arranged into inclined planes; the shock absorption step by using the shock absorption device comprises the following steps that the device is placed on an automobile, when the automobile runs on a bumpy road, the base transmits upward impact force to the third telescopic spring, the third telescopic spring drives the telescopic cylindrical block 75 to move upwards, the telescopic cylindrical block 75 drives the second telescopic spring to move upwards, the telescopic rod 4 is driven to move close to the inner wall of the liquid nitrogen shell, and the first stretching spring is used for absorbing shock, so that the shock caused by bumping of the automobile can be reduced, the shock to which liquid nitrogen and semen are subjected is reduced as much as possible, and the liquid nitrogen explosion or sperm death caused by over-great shock is prevented; thereby can consume the impact force that the vehicle jolted and brought through utilizing flexible cylinder piece 75 rebound promotion telescopic link to remove all around to can strengthen the shock attenuation effect.
The liquid nitrogen adding device comprises an air outlet hole arranged on the inner container 2, a first round pipe 7 fixedly arranged in the air outlet hole, a supporting plate 8 fixedly arranged on the right end surface of the first round pipe 7, a first rotating shaft rotatably arranged on the right end surface of the supporting plate 8, a first fan 10 fixedly arranged on the first rotating shaft, a first chain wheel fixedly arranged on the first rotating shaft, a moving block 12 movably arranged in the first round pipe 7, a plurality of air vents arranged on the inner wall of the first round pipe 7 in the circumferential direction and communicated with the outside, a support rod 13 fixedly arranged on the left end surface of the moving block 12, a push rod 14 fixedly arranged on the left end surface of the support rod 13, a liquid inlet hole arranged on the lower side of the air outlet hole, a second round pipe 15 fixedly arranged in the liquid inlet hole, a rotating hole arranged on the upper side of the inner wall of the second round pipe 15, a rotating rod 16 rotatably arranged on the rotating hole, a first rotating plate 17 fixedly arranged on the upper end of the rotating rod 16, A rotary disc 318 fixedly arranged on the rotary rod 16 and positioned in the second round tube 15, a second rotary plate 18 fixedly arranged at the lower end of the rotary rod 16, a matching hole arranged at the lower side of the liquid inlet hole, a straight rod 19 movably arranged in the matching hole, a supporting plate fixedly arranged at the right end surface of the second round tube 15, a second rotary shaft rotatably arranged at the right end surface of the supporting plate, a second fan 21 fixedly arranged at the right end of the second rotary shaft, a second chain wheel 22 fixedly arranged at the outer surface of the second rotary shaft 20, a transmission chain 23 in transmission connection between the first chain wheel and the second chain wheel 22, a feeding shell 24 fixedly arranged at the left end surface of the second round tube 15, a feeding space arranged in the feeding shell 24 and opened towards the left, two sliding trapezoidal grooves respectively arranged at the left end surface of the feeding shell 24, a movable plate 26 vertically movably arranged at the left end surface of the feeding shell 24, two sliding trapezoidal blocks fixedly arranged at the upper lower side and the right end surface of the movable plate 26 and capable of vertically sliding in the two sliding trapezoidal grooves, The connecting pipeline 27 is fixedly arranged on the left end face of the moving plate 26 and penetrates through the moving plate 26, and a position sensor is fixedly arranged in the connecting pipeline 318; the liquid nitrogen adding method comprises the following steps of adding liquid nitrogen by using a liquid nitrogen adding device; an external liquid nitrogen storage tank is connected with the external liquid nitrogen storage tank 27 through pipeline threaded connection, when liquid nitrogen in the inner container is vaporized, the pressure in the inner container is increased, when the pressure reaches a certain degree, the moving block 12 is pushed to move leftwards, the moving block 12 drives 14 to move leftwards, so that the moving block 17 is driven to rotate ninety degrees to reach the left side, meanwhile, the moving block 318 is driven to rotate ninety degrees, when the position sensor senses that the position sensor is started after the moving block 318 rotates ninety degrees, the position sensor drives the water pump to start, the water pump inputs the liquid nitrogen in the liquid nitrogen storage tank into the inner container, the liquid nitrogen drives 21 to rotate, the liquid nitrogen drives 22 to rotate, the first chain wheel 22 drives the first chain wheel to rotate, the first chain wheel drives 10 to rotate, so that the nitrogen can be rapidly discharged through the rotation of 10, the lifting plate can be driven to move downwards through the entering of the liquid nitrogen, when the lifting plate moves downwards and touches 19, the lifting plate can drive 19 to move rightwards, and the lifting plate can drive 18 to reach the left side through the rotation from the front side, 18 can drive 16 to rotate, 16 can drive 17 to rotate from the left side by ninety degrees to the rear side, so that 17 can drive 14 to move rightwards in the rotating process, 14 can drive 12 to move rightwards to reset and seal the first round pipe again, and the liquid nitrogen tank can keep low temperature to prevent sperms from being influenced in transportation; nitrogen can be discharged as soon as possible through the rotation of the valve 10, and explosion caused by overlarge internal air pressure due to the fact that the nitrogen is accumulated in the liner excessively is avoided; the arrangement of the 26 can prevent the inner container from vibrating when the inner container vibrates up and down slightly and can keep the liquid nitrogen in a sealing state all the time, so that the liquid nitrogen leakage caused by cracking of the 27 when the 27 is bumped by a vehicle can be avoided.
The anti-toppling device comprises a matching cylindrical shell 28 fixedly arranged on the upper end surface of the lifting plate, a cylindrical space arranged on the upper end surface of the matching cylindrical shell 28, a first matching shell 29 fixedly arranged on the lower end wall of the cylindrical space, a connecting circular plate 30 fixedly arranged on the upper end surface of the first matching shell 29, and a plurality of extrusion springs 70 fixedly arranged on the inner wall of the connecting circular plate 30 in the circumferential direction respectively, the second matching shell 31 is fixedly arranged at the upper end of the connecting circular ring plate 30, the plurality of first vent holes are respectively arranged in the first matching shell 29 in the circumferential direction, the first abutting rods 32 are movably arranged in the plurality of first vent holes, the plurality of second vent holes are respectively arranged in the second matching shell 31 in the circumferential direction, and the second abutting rods 33 are movably arranged in the plurality of second vent holes, wherein each first vent hole is communicated with the second vent hole on the opposite side; when the lifting barrel is inclined to the periphery under the action of vibration, the first abutting rod 32 in contact with the lifting barrel moves towards the first air vent, the second abutting rod 33 opposite to the first abutting rod moves leftwards to push the lower side of the lifting barrel to move leftwards, so that the upper side and the lower side of the lifting barrel can be prevented from inclining, the semen in the lifting barrel can be poured out, meanwhile, the lifting barrel can be damped through gas, and the practicability is higher; when the lifting barrel is pushed to be close to the inner wall by the first abutting rod, the spring positioned on the inner wall can push the lifting barrel to be far away from the inner wall, so that the phenomenon that the lifting barrel abuts against the inner wall of the cylindrical space when the lifting barrel vibrates in one direction all the time and cannot play a role in damping is avoided.
The external fixed surface of 318 is provided with the ultra-low temperature sealing washer, the ultra-low temperature sealing washer is prior art, and the event is no longer described any more.
The working principle is as follows: the method for transporting the liquid nitrogen by using the liquid nitrogen transportation equipment comprises the following steps: put into liquid nitrogen transportation equipment with the seminal fluid in, carry out the shock attenuation to liquid nitrogen and seminal fluid through damping device in the transportation, add the device through liquid nitrogen and can add the liquid nitrogen automatically when liquid nitrogen is not enough in liquid nitrogen transportation equipment.

Claims (5)

1. A method for extracting bovine semen is characterized in that: the method comprises the following steps of,
a. and (3) bull training: selecting a robust bull to climb across a cow so as to form a conditioned reflex;
b. preparing before semen collection: preparing 75% of disinfecting alcohol;
c. preparing a false vagina: a disinfected glass rod is stuck with Vaseline and evenly smeared on one half of the inner tube of the false vagina to increase the lubrication degree;
d. loosening the bull: utilize fluorescent lamp to shine the bull and make the bull relax muscle
e. And (3) semen collection: allowing the bull to climb onto a Taihe, and plugging testis of the bull into the artificial vagina to finish semen collection;
f. and (3) transporting semen: putting the semen into liquid nitrogen transportation equipment and then transporting the semen by using an automobile;
the liquid nitrogen transportation equipment in the step f comprises liquid nitrogen outer shells (1), inner containers (2) arranged in the liquid nitrogen outer shells (1), a plurality of lifting cylinders (100) arranged in the inner containers (2), a damping device arranged on the lower sides of the liquid nitrogen outer shells, and liquid nitrogen adding devices arranged on the inner containers (2); an anti-toppling device arranged on the lifting cylinder (100);
the anti-toppling device comprises a matching cylindrical shell (28) fixedly arranged on the upper end surface of the lifting plate, a cylindrical space arranged on the upper end surface of the matching cylindrical shell (28), a first matching shell (29) fixedly arranged on the lower end wall of the cylindrical space, a connecting circular plate (30) fixedly arranged on the upper end surface of the first matching shell (29), and a plurality of extrusion springs (70) fixedly arranged on the inner wall of the connecting circular plate (30) in the circumferential direction respectively, the second matching shell (31) is fixedly arranged at the upper end of the connecting circular ring plate (30), the plurality of first vent holes are respectively arranged in the first matching shell (29) in the circumferential direction, the first abutting rods (32) are movably arranged in the plurality of first vent holes, the plurality of second vent holes are respectively arranged in the second matching shell (31) in the circumferential direction, and the second abutting rods (33) are movably arranged in the plurality of second vent holes; each first vent hole is communicated with a second vent hole positioned on the opposite side of the first vent hole;
the method for transporting the liquid nitrogen by using the liquid nitrogen transportation equipment comprises the following steps: the semen is placed in liquid nitrogen transportation equipment, liquid nitrogen and the semen are subjected to shock absorption in transportation through a shock absorption device, and liquid nitrogen can be automatically added through a liquid nitrogen adding device when the liquid nitrogen in the liquid nitrogen transportation equipment is insufficient;
the liquid nitrogen adding device comprises an air outlet hole arranged on the inner container (2), a first circular tube (7) fixedly arranged in the air outlet hole, a support plate (8) fixedly arranged on the right end face of the first circular tube (7), a first rotating shaft rotatably arranged on the right end face of the support plate (8), a first fan (10) fixedly arranged on the first rotating shaft, a first chain wheel fixedly arranged on the first rotating shaft, a moving block (12) movably arranged in the first circular tube (7), a plurality of air holes which are circumferentially arranged on the inner wall of the first circular tube (7) and communicated with the outside, a support rod (13) fixedly arranged on the left end face of the moving block (12), a push rod (14) fixedly arranged on the left end face of the support rod (13), a liquid inlet hole arranged on the lower side of the air outlet hole, a second circular tube (15) fixedly arranged in the liquid inlet hole, and a rotating hole arranged on the upper side of the inner wall of the second circular tube (15), A rotating rod (16) which is rotatably arranged on the rotating hole, a first rotating plate (17) which is fixedly arranged at the upper end of the rotating rod (16), a rotating disc (318) which is fixedly arranged on the rotating rod (16) and is positioned in a second round pipe (15), a second rotating plate (18) which is fixedly arranged at the lower end of the rotating rod (16), a matching hole which is arranged at the lower side of the liquid inlet hole, a straight rod (19) which is movably arranged in the matching hole, a supporting plate which is fixedly arranged at the right end surface of the second round pipe (15), a second rotating shaft which is rotatably arranged at the right end surface of the supporting plate, a second fan (21) which is fixedly arranged at the right end of the second rotating shaft, a second chain wheel (22) which is fixedly arranged at the outer surface of the second rotating shaft (20), a transmission chain (23) which is in transmission connection between the first chain wheel and the second chain wheel (22), a feeding shell (24) which is fixedly arranged at the left end surface of the second round pipe (15), a feeding space which is arranged in the feeding shell (24) and has an opening facing left, The feeding device comprises two sliding trapezoidal grooves respectively arranged on the left end face of a feeding shell (24), a moving plate (26) arranged on the left end face of the feeding shell (24) in a vertically movable mode, two sliding trapezoidal blocks fixedly arranged on the upper side and the lower side of the right end face of the moving plate (26) and capable of sliding vertically in the two sliding trapezoidal grooves, and a connecting pipeline (27) fixedly arranged on the left end face of the moving plate (26) and penetrating through the moving plate (26), wherein a position sensor is fixedly arranged in a rotating disc (318);
the liquid nitrogen adding method comprises the following steps of adding liquid nitrogen by using a liquid nitrogen adding device; an external liquid nitrogen storage tank is connected with a connecting pipeline (27) through pipeline threaded connection, when liquid nitrogen in the inner container is vaporized, the pressure in the inner container can be increased, when the pressure reaches a certain degree, a moving block (12) can be pushed to move leftwards, the moving block (12) can drive a push rod (14) to move leftwards, so that a first rotating plate (17) is driven to rotate ninety degrees to reach the left side, a rotating disc (318) is driven to rotate ninety degrees at the same time, when a position sensor senses that the rotating disc (318) rotates ninety degrees, a position sensor is started, the position sensor drives a water pump to be started, the water pump inputs liquid nitrogen in the liquid nitrogen storage tank into the inner container, the liquid nitrogen can drive a second fan (21) to rotate, the second fan (21) drives a second chain wheel (22) to rotate, the second chain wheel (22) drives a first chain wheel to rotate, and the first chain wheel drives a first fan (10) to rotate, thereby can discharge nitrogen gas fast through the rotation of first fan (10), the entering through the liquid nitrogen can drive the lifter plate and move down, can drive straight-bar (19) and move to the outside when straight-bar (19) is met in the lifter plate downstream, straight-bar (19) can drive second rotor plate (18) and arrive the left side from the rotatory ninety degrees in front of the side, second rotor plate (18) can drive rotor plate (16) rotatory, rotor plate (16) can drive first rotor plate (17) and arrive the rear side from the rotatory ninety degrees in left side, thereby first rotor plate (17) can promote push rod (14) and move right at the rotatory in-process, push rod (14) can promote movable block (12) and move right and reset and again inclosed with first pipe, thereby can make the interior low temperature of liquid nitrogen jar prevent that the sperm from receiving the influence in the transportation.
2. The method for extracting bovine semen according to claim 1, wherein: the 75% disinfecting alcohol comprises anhydrous alcohol, medical vaseline oil, medical cotton and soap.
3. The method for extracting bovine semen according to claim 1, wherein: when in semen collection, the artificial vagina is held by the right hand to form an angle of 40 degrees with the Taihu, the entrance is inclined downwards, and the penis of the bull is naturally introduced into the artificial vagina by the cooperation of the left hand and the right hand.
4. The method for extracting bovine semen according to claim 1, wherein: the artificial vagina for semen collection is composed of a shell, an inner tube, a funnel and a semen collection tube.
5. The method for extracting bovine semen according to claim 1, wherein: the shock absorption device comprises a telescopic pipe (3) fixedly arranged on the lower end face of a liquid nitrogen shell, a plurality of holes formed in the outer surface of the telescopic pipe (3) in the circumferential direction, a plurality of telescopic rods (4) movably arranged on the holes respectively, a first telescopic spring fixedly arranged between the inner wall of the liquid nitrogen shell and the telescopic rods (4), a telescopic cylindrical block (75) arranged in the telescopic pipe (3) in a vertically movable mode, a second telescopic spring fixedly arranged between the lower end face of the liquid nitrogen shell (1) and the telescopic cylindrical block (75), a base fixedly arranged at the lower end of the telescopic cylindrical block (75), a fixed pipe (76) fixedly arranged on the upper surface of the base, and a third telescopic spring fixedly arranged between the bottom of the fixed pipe (76) and the telescopic cylindrical block (75), wherein one ends, opposite to the telescopic rods, are arranged to form an inclined plane;
the step of utilizing damping device to carry out the absorbing is as follows, put this equipment on the car, when the car goes on the road of jolting, the base will make progress impact force transmit the third expanding spring on, third expanding spring drives flexible cylinder piece (75) rebound, flexible cylinder piece (75) drive second expanding spring rebound, drive telescopic link (4) and be close to the inner wall removal of liquid nitrogen shell, reach through first extension spring and absorb vibrations.
CN201911247274.XA 2019-12-09 2019-12-09 Bovine semen extraction method Active CN111110393B (en)

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