CN111088300A - 一种冠突散囊菌黑色素发酵制备方法 - Google Patents
一种冠突散囊菌黑色素发酵制备方法 Download PDFInfo
- Publication number
- CN111088300A CN111088300A CN202010120095.6A CN202010120095A CN111088300A CN 111088300 A CN111088300 A CN 111088300A CN 202010120095 A CN202010120095 A CN 202010120095A CN 111088300 A CN111088300 A CN 111088300A
- Authority
- CN
- China
- Prior art keywords
- melanin
- eurotium cristatum
- molecules
- mol
- precipitate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 title claims abstract description 176
- 241001205401 Aspergillus cristatus Species 0.000 title claims abstract description 30
- 238000000855 fermentation Methods 0.000 title claims abstract description 27
- 230000004151 fermentation Effects 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 24
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000002244 precipitate Substances 0.000 claims abstract description 14
- 241001122767 Theaceae Species 0.000 claims abstract description 7
- 238000001556 precipitation Methods 0.000 claims abstract description 6
- 238000004090 dissolution Methods 0.000 claims abstract description 5
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 5
- 239000012498 ultrapure water Substances 0.000 claims abstract description 5
- 238000004108 freeze drying Methods 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims description 14
- 230000001376 precipitating effect Effects 0.000 claims description 8
- 238000009630 liquid culture Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 239000000287 crude extract Substances 0.000 claims 1
- 239000006228 supernatant Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 238000005119 centrifugation Methods 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 3
- 239000008280 blood Substances 0.000 abstract description 3
- 210000004369 blood Anatomy 0.000 abstract description 3
- 238000012512 characterization method Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 241000233866 Fungi Species 0.000 description 10
- 238000010521 absorption reaction Methods 0.000 description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 4
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 230000003647 oxidation Effects 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 239000011790 ferrous sulphate Substances 0.000 description 2
- 235000003891 ferrous sulphate Nutrition 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000001103 potassium chloride Substances 0.000 description 2
- 235000011164 potassium chloride Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 235000010344 sodium nitrate Nutrition 0.000 description 2
- 239000004317 sodium nitrate Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229960004793 sucrose Drugs 0.000 description 2
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 206010016766 flatulence Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 238000012844 infrared spectroscopy analysis Methods 0.000 description 1
- 238000002329 infrared spectrum Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000008099 melanin synthesis Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- -1 polyphenol compounds Chemical class 0.000 description 1
- 150000008442 polyphenolic compounds Polymers 0.000 description 1
- 235000013824 polyphenols Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000041 toxicology testing Toxicity 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/10—Nitrogen as only ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B67/00—Influencing the physical, e.g. the dyeing or printing properties of dyestuffs without chemical reactions, e.g. by treating with solvents grinding or grinding assistants, coating of pigments or dyes; Process features in the making of dyestuff preparations; Dyestuff preparations of a special physical nature, e.g. tablets, films
- C09B67/0096—Purification; Precipitation; Filtration
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
本发明提供一种冠突散囊菌黑色素发酵制备方法，从茯砖茶中分离的高产黑色素的冠突散囊菌株发酵液除去菌丝体后，加入3 mol/L盐酸使pH=3，低温下放置4h，黑色素分子自然沉淀出来，离心后收集存留在沉淀中的黑色素，用0.01 mol/L的NaOH溶液溶解黑色素，再次加入3 mol/L盐酸使pH=3，沉淀黑色素分子，通过3‑5次的沉淀和复溶解过程，黑色素分子得到纯化，最后一次的沉淀用超纯水洗涤3~5次后，冷冻干燥使得黑色素分子彻底干燥。黑色素分子通过结构表征证实为真黑色素，进一步的动物实验表明冠突散囊菌的黑色素具有一定的降血脂的功效。
Description
技术领域
本发明涉及微生物培养与发酵，具体是一种冠突散囊菌黑色素发酵制备方法。
背景技术
黑色素是目前应用最多最广的生物天然色素之一，黑色素主要是由多羟基酚、多羟基吲哚与吡咯氧化而成的构造不规则的化合物，在细胞中常与蛋白质等物质结合存在，微溶或难溶于水，不溶于酸溶液和常见有机溶剂，可溶于碱溶液。天然黑色素包括两大类，一类是酪氨酸、多酚化合物经生物代谢所得最终产物黑色素(melanin)；另外一大类为花苷类颜色较深的黑色素，主要分布于植物中。根据黑色素中氮、硫元素的含量，将黑色素分为真黑色素、异黑色素和棕黑色素三类。
从不同生物分离到的黑色素均被报道具有较强的抗氧化、 清除自由基等功效，且具有延缓衰老、舒气止胀、清热解毒、壮阳补肾、软化血管、抗肥胖、提高免疫力等功效，还可防治各种疾病，如高血压、心脏病、糖尿病、血管硬化、癌症及肝脏疾病等。传统的黑色素合成主要釆用酶法和化学法，但是由于原料以及酪氨酸酶价格昂贵，合成流程复杂，成本较高，不利于大规模工厂化生产。微生物来源的黑色素因为微生物的易培养、得率高等特点，逐渐被学者们挖掘，有许多学者利用细菌、霉菌或者真菌生产黑色素，考虑到菌种安全性，大多数菌株 （如： 大肠杆菌、苏云金芽孢杆菌等）生产的黑色素在食品中应用还有一些障碍。
冠突散囊菌（Eurotium cristatum）又名金花菌，是茯砖茶制作过程中，在特殊的温度、湿度等环境调节下，通过“发花”过程而生长起来的自然的有益的一种真菌。冠突散囊菌自从茯砖茶中分离出后，很多人对其毒性、生理生化活性和茶叶制作进行了研究。多种动物中开展的毒理学研究表明，金花菌属于无毒微生物，而且其发酵产物还具有很好的抗氧化、抑菌、调节肠道菌群平衡、提高免疫力、抗衰老和抗肿瘤等活性。在培养中发现冠突散囊菌在液体发酵中经过适当处理能产生较高产量的黑色素，该黑色素分子结构与一些大型真菌如黑木耳中黑色素的结构基本一致，且具有很强的抗氧化和降血脂的功能。
发明内容
本发明的目的在于提供一种冠突散囊菌黑色素发酵制备方法。
通过从茯砖茶中分离出的优质高产黑色素的冠突散囊菌株，通过摇瓶发酵可产黑色素。提取发酵液中的黑色素并测定其含量。
本发明采用的技术方案是：
一种冠突散囊菌黑色素发酵制备方法，包括以下步骤：将从茯砖茶中分离的高产黑色素的冠突散囊菌株发酵液除去菌丝体后，加入3 mol/L盐酸使pH=3，低温下放置4h，黑色素分子自然沉淀出来，离心后收集存留在沉淀中的黑色素，用0.01 mol/L的NaOH溶液溶解黑色素，再次加入3 mol/L盐酸使pH=3，沉淀黑色素分子，通过3-5次的沉淀和复溶解过程，黑色素分子得到纯化，最后一次的沉淀用超纯水洗涤3~5次后，冷冻干燥使得黑色素分子彻底干燥。黑色素分子通过结构表征证实为真黑色素，进一步的动物实验表明冠突散囊菌的黑色素具有一定的降血脂的功效。
具体为：将获得的冠突散囊菌株接入灭菌的液体培养基中，液体培养基培养为：硝酸钠3g、磷酸氢二钾1g、硫酸镁0.5g、氯化钾0.5g、硫酸亚铁0.01g、蔗糖30g、蒸馏水1 L、pH为 5.5。培养条件28℃，180r/min，当培养到5天的时候培养瓶中会产生大量的冠突散囊菌的菌丝球，此时将液体培养基和菌丝球放置到4℃冰箱内静置8-10h，然后继续28℃，180r/min培养第二天就会观察到有大量黑色素被分泌到培养基中来，培养基开始变为黑褐色。当培养到第10天时候终止培养，收获黑色素。
通过离心或过滤除去发酵液中菌丝体后，加入3 mol/L盐酸使除去菌丝体的发酵液的pH=3，低温下放置4h，黑色素分子自然沉淀出来，8000r/min离心10min后收集呈现黑色的沉淀，用0.01 mol/L的NaOH溶液溶解黑色素，再次加入3 mol/L盐酸使pH=3，沉淀黑色素分子，再次用0.01 mol/L的NaOH溶液溶解黑色素沉淀，反复5次沉淀和溶解过程，使得黑色素分子得到纯化，最后一次的沉淀用超纯水洗涤3~5次后，冷冻干燥黑色素分子。
经红外质谱测定发现，该黑色素结构与黑木耳的黑色素结构相似。
本发明的优点在于：通过从冠突散囊菌菌株发酵液中提取黑色素方法，提高了黑色素产量。冠突散囊菌比一般真菌生长速度快，易培养，采用冠突散囊菌菌株发酵法制备黑色素，缩短黑色素提取时间、成本低、产量高，得到黑色素的率为6%左右，适用于大规模工厂化生产，并可促进其后续生物产品的开发。
附图说明
图1冠突散囊菌的黑色素红外光谱图。
具体实施方式
实施例1
将冠突散囊菌株接入灭过菌的400 ml的液体培养基中（1L培养瓶），液体培养基的配方为：硝酸钠3g、磷酸氢二钾1g、硫酸镁0.5g、氯化钾0.5g、硫酸亚铁0.01g、蔗糖30g、蒸馏水1L、pH为 5.5，接种量为1%。放入全温摇床中培养，培养条件28℃，180r/min，当培养到5天的时候培养瓶中会产生大量的冠突散囊菌的菌丝球，此时将液体培养基和菌丝球放置到4℃冰箱内静置8-10h，然后继续放入全温摇床28℃，180r/min培养，第二天就会观察到有大量黑色素分子被分泌到培养基中来，培养基开始变为黑褐色，当培养到第10天时终止培养，准备收获黑色素。
通过离心或过滤除去发酵液中菌丝体后，加入3 mol/L盐酸使除去菌丝体的发酵液的pH=3，低温下放置4h，黑色素分子自然沉淀出来，8000r/min离心10min后收集呈现黑色的沉淀，用0.01 mol/L的NaOH溶液溶解黑色素，再次加入3 mol/L盐酸使pH=3，沉淀黑色素分子，再次用0.01 mol/L的NaOH溶液溶解黑色素沉淀，反复5次沉淀和溶解过程，使得黑色素分子得到纯化，最后一次的沉淀用超纯水洗涤3~5次后，冷冻干燥黑色素分子。经红外质谱测定发现，该黑色素结构与部分大型真菌的黑色素相似，且产率高。
对提取到的黑色素进行红外光谱分析发现其结构有以下特征：
1) 在波数3407.8 cm-1 处都有较宽而强的吸收峰，说明存在－NH 结构；1626.2 cm-1处吸收峰是C=O 的非对称振动及N－H变角振动形成，说明可能存在－COOH；1447 .2 cm-1处的吸收峰是自由羧基基团－COOH引起的振动；1383.5 cm-1 处吸收峰是C－CH3 的弯曲和骨架振动吸收参与形成；600～700 cm-1范围的吸收带没有吸收峰，表明苯环被取代，形成了共轭体系。
2) 冠突散囊菌的黑色素在波数2933.1 cm-1处有吸收峰可能是由于C－H伸缩振动形成，即为－CH和－CH2 的共振吸收峰。
以上所述仅为本发明的较佳实施例，凡依本发明申请专利范围所做的均等变化与修饰，皆应属本发明的涵盖范围。
Claims (2)
1.一种冠突散囊菌黑色素发酵制备方法，其特征在于：将从茯砖茶中中分离的高产黑色素冠突散囊菌菌株发酵液离心或过滤除去菌丝体，用盐酸调整pH至3.0，将黑色素从发酵液上清中沉淀出来，离心收集沉淀得到黑色素粗提物，再进一步纯化。
2.根据权利要求1所述的一种冠突散囊菌黑色素发酵制备方法，其特征在于：具体方法为将冠突散囊菌接入液体培养基中，全温摇床中28℃，160r/min摇瓶发酵5天，可以观察到冠突散囊菌大量菌丝球的生长，将培养的菌丝球和培养基放置入4℃静置8h，然后再放入全温摇床培养5天，可以看到培养基明显呈现黑色； 除去发酵液中菌丝体，将发酵液用3 mol/L盐酸调整pH=3，低温下放置4h，黑色素分子自然沉淀出来，8000r/min离心10min后收集呈现黑色的沉淀，用0.01 mol/L的NaOH溶液溶解黑色素，再次加入3 mol/L盐酸使pH=3，沉淀黑色素分子，再次用0.01 mol/L的NaOH溶液溶解黑色素沉淀，反复5次沉淀和溶解过程，使得黑色素分子得到纯化，最后一次的沉淀用超纯水洗涤3~5次后，冷冻干燥黑色素分子，得到黑色素。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010120095.6A CN111088300A (zh) | 2020-02-26 | 2020-02-26 | 一种冠突散囊菌黑色素发酵制备方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010120095.6A CN111088300A (zh) | 2020-02-26 | 2020-02-26 | 一种冠突散囊菌黑色素发酵制备方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111088300A true CN111088300A (zh) | 2020-05-01 |
Family
ID=70400234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010120095.6A Pending CN111088300A (zh) | 2020-02-26 | 2020-02-26 | 一种冠突散囊菌黑色素发酵制备方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111088300A (zh) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111635915A (zh) * | 2020-06-29 | 2020-09-08 | 中南林业科技大学 | 一种冠突散囊菌黑色素发酵产物的制备方法与应用 |
| CN111778291A (zh) * | 2020-06-23 | 2020-10-16 | 南京师范大学 | 一种利用微生物或酶发酵或转化葵粕和葵壳增产黑色素的方法 |
| CN112194910A (zh) * | 2020-09-27 | 2021-01-08 | 中北大学 | 一种天然黑色素的提取方法 |
| CN113549657A (zh) * | 2021-06-18 | 2021-10-26 | 南京师范大学 | 一种利用蓝草或蓝草加工剩余物制备黑色素的方法 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0363792A1 (en) * | 1988-10-03 | 1990-04-18 | Biosource Genetics Corporation | Melanin production |
| WO1992000373A1 (en) * | 1990-06-29 | 1992-01-09 | Biosource Genetics Corporation | Melanin production by transformed microorganisms |
| US5631151A (en) * | 1988-10-03 | 1997-05-20 | Biosource Technologies, Inc. | Melanin production by transformed organisms |
| CN103205464A (zh) * | 2013-04-16 | 2013-07-17 | 南京师范大学 | 散囊菌利用内醚糖及纤维素热解液等产黑色素的方法 |
| CN109288014A (zh) * | 2018-10-29 | 2019-02-01 | 南京林业大学 | 一种白果冠突散囊菌液态发酵的方法及其制备的产品和应用 |
-
2020
- 2020-02-26 CN CN202010120095.6A patent/CN111088300A/zh active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0363792A1 (en) * | 1988-10-03 | 1990-04-18 | Biosource Genetics Corporation | Melanin production |
| US5631151A (en) * | 1988-10-03 | 1997-05-20 | Biosource Technologies, Inc. | Melanin production by transformed organisms |
| WO1992000373A1 (en) * | 1990-06-29 | 1992-01-09 | Biosource Genetics Corporation | Melanin production by transformed microorganisms |
| CN103205464A (zh) * | 2013-04-16 | 2013-07-17 | 南京师范大学 | 散囊菌利用内醚糖及纤维素热解液等产黑色素的方法 |
| CN109288014A (zh) * | 2018-10-29 | 2019-02-01 | 南京林业大学 | 一种白果冠突散囊菌液态发酵的方法及其制备的产品和应用 |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111778291A (zh) * | 2020-06-23 | 2020-10-16 | 南京师范大学 | 一种利用微生物或酶发酵或转化葵粕和葵壳增产黑色素的方法 |
| CN111635915A (zh) * | 2020-06-29 | 2020-09-08 | 中南林业科技大学 | 一种冠突散囊菌黑色素发酵产物的制备方法与应用 |
| CN112194910A (zh) * | 2020-09-27 | 2021-01-08 | 中北大学 | 一种天然黑色素的提取方法 |
| CN112194910B (zh) * | 2020-09-27 | 2022-04-26 | 中北大学 | 一种从霉菌中提取天然黑色素的方法 |
| CN113549657A (zh) * | 2021-06-18 | 2021-10-26 | 南京师范大学 | 一种利用蓝草或蓝草加工剩余物制备黑色素的方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111088300A (zh) | 一种冠突散囊菌黑色素发酵制备方法 | |
| CN107197966B (zh) | 一种微生物发酵制作gaba茶的方法 | |
| CN111317694B (zh) | 一种杜仲发酵提取液及其制备方法和其在化妆品中的应用 | |
| CN109251954B (zh) | 一种海参多肽的生产方法 | |
| CN107418995B (zh) | 一种石榴皮单宁液态发酵制备的鞣花酸及其制备方法 | |
| CN101736055A (zh) | 一种黑木耳多糖的提取方法 | |
| CN110066834A (zh) | 一种白藜芦醇的提取方法 | |
| CN110438015B (zh) | 产橙皮苷酶的枳实内生真菌及其发酵产橙皮苷酶的方法 | |
| CN109810909B (zh) | 一株高产番茄红素的红发夫酵母菌株及番茄红素生产方法 | |
| WO2017016199A1 (zh) | 一种沙链霉菌的用途及香兰素的生产方法 | |
| CN111961698A (zh) | 一种利用短乳杆菌产酶降解西番莲果皮多糖的制备方法及其应用 | |
| KR100643735B1 (ko) | 두릅나무과 관목의 과실을 주재료로 하는 발효 과실주 및그 제조방법 | |
| CN107099489B (zh) | 一株提高竹红菌素发酵产率的伴生细菌菌株及其应用 | |
| CN109369240A (zh) | 一种促进植物光合作用的有机叶面肥及其制备方法 | |
| CN112159827B (zh) | 一种琼胶寡糖的生产工艺 | |
| CN115417935A (zh) | 一种海藻硒多糖的制备方法 | |
| CN111733104B (zh) | 一种红茶菌蛋白的制备新方法及其应用 | |
| CN114591847A (zh) | 一种提高虫草素含量的蛹虫草菌培养基组合物、液体发酵方法及虫草素制备方法 | |
| CN105624229B (zh) | 一种提高尼莫克汀产量的方法 | |
| CN103125822A (zh) | 糙米功能红曲醪糟的制备方法 | |
| CN113149819B (zh) | 一种从竹黄菌固态发酵料中提取竹红菌素的方法 | |
| CN111333742A (zh) | 一种紫菜多糖的制备方法 | |
| KR0149831B1 (ko) | 복합효소의 반응으로 생성되는 건강식품 및 그 제조방법 | |
| CN114606139B (zh) | 一种爪哇青霉菌及其发酵产物和应用 | |
| CN108048427B (zh) | 一种单宁酶固态发酵培养基制备方法及其应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20200501 |
|
| WD01 | Invention patent application deemed withdrawn after publication |