CN111085281B - Surface acoustic wave regulated high-flux micro-droplet generation device and method - Google Patents
Surface acoustic wave regulated high-flux micro-droplet generation device and method Download PDFInfo
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Abstract
A surface acoustic wave regulated high-throughput micro-droplet generation device and a surface acoustic wave regulated high-throughput micro-droplet generation method are disclosed, the device comprises an interdigital transducer, wherein an arc interdigital electrode is manufactured on the interdigital transducer, a PDMS micro-channel system is bonded on the upper part of the interdigital transducer, and the PDMS micro-channel system is formed by bonding a top PDMS channel, a middle PDMS channel and a bottom PDMS channel; the method is that the device is fixed on the objective table of the microscope, the disperse phase and the continuous phase are adjusted to corresponding flow velocity through the injection pump, the disperse phase is cut into micro-droplets by the continuous phase at each T-shaped micro-channel in the device, then an output button of a signal generator is pressed, convergent surface acoustic waves are generated on an interdigital transducer, an acoustic pressure field acts on the continuous phase to increase corresponding flow resistance, and the acoustic pressure field is used as an acoustic valve to regulate and control the flow velocity of the continuous phase, thereby realizing the regulation and control of the size of the droplets; the invention ensures the high flux generation of the liquid drops, adjusts the amplitude and the frequency of the input sinusoidal voltage, and can flexibly regulate and control the generation size of the micro liquid drops in real time.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to a high-flux micro-droplet generation device and method regulated and controlled by surface acoustic waves.
Background
Microdroplets are used in biomedical research and chemical material manufacturing in a wide range of applications, where microdroplet generation methods are of paramount importance, with different methods varying widely in terms of drop generation rate and uniformity. With the development of microfluidic technology, passive micro-droplet generation methods and active micro-droplet generation methods have appeared. At present, the passive generation method of micro-droplets mainly comprises a co-flow method, a flow type convergence method, a T-shaped micro-flow channel method and a derivation method based on the methods, the dispersed phase can be rapidly cut into micro-droplets by the continuous phase by adjusting the input pressure of the dispersed phase and the continuous phase, and the liquid phase is usually oil and water solution. However, in most passive droplet generation devices, the pressure source is usually far away from the microfluidic chip and needs to be connected to the microfluidic chip through a long connecting tube, the compressibility of the fluid or channel material causes a time delay, and if it needs to be adjusted in real time as required when adjusting the droplet size, the above method has some limitations and needs a long system response time to stabilize the droplet generation.
In order to solve the problems, a micro-droplet generation method combining external forces such as magnetic control, electric control and sound control with the passive method is provided, and the generation size of micro-droplets can be flexibly regulated and controlled in real time through the external forces. There are studies on the generation of droplets related to surface acoustic waves that have been made by Jason c. brenker and David j. collins that generate surface acoustic waves by applying high frequency SAW as a pressure source to a two-phase flow interface in a microchannel by applying a timed pulse voltage (see Jason c. brenker, David j. collins, Hoang Van Phan, tuncaan and Adrian Neild. Lab Chip,2016,16,1675-1683.David j. collins, tuncaan, krian Helmerson and Adrian Neild, Lab Chip,2013,13, 3225-. Lothar Schmid uses surface acoustic waves as a power source, micro-droplets with controllable sizes are generated in a T-shaped flow channel (see Lothar Schmid and Thomas Franke. applied Physics Letters 104,133501 (2014)), and the size of the generated droplets is controlled by applying SAW to a flow type droplet converging and generating device (see Lothar Schmid and Thomas Franke. Lab Chip,2013,13, 1691-.
However, in the above method for generating acoustic control droplets, most of the used microfluidic devices are two-dimensional flow channels, the generation rate of the microfluidic devices is limited to a certain extent, and the method is not suitable for generating large-flux micro droplets, and particularly when large-scale droplets are required to be rapidly generated, the generation rate is difficult to meet the requirement, and a high-flux microfluidic generation device for actively regulating and controlling the droplets in real time is urgently needed.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide a surface acoustic wave regulated high-flux micro-droplet generation device and a surface acoustic wave regulated high-flux micro-droplet generation method, wherein the high-flux generation of micro-droplets is realized through the annular array arrangement of a T-shaped micro-channel structure, and the size of the micro-droplets can be flexibly regulated through the input voltage and frequency of the surface acoustic wave generated by an interdigital transducer integrated in the device; the method is not only suitable for high-pass generation of micro-droplets, but also suitable for high-pass generation of micro-bubbles.
In order to achieve the purpose, the invention adopts the following technical scheme:
a high-flux micro-droplet generating device regulated by surface acoustic waves comprises an interdigital transducer 1000, wherein an arc electrode is manufactured on the interdigital transducer 1000, a PDMS micro-channel system is bonded on the upper part of the interdigital transducer 1000, and the arc electrode is matched with the PDMS micro-channel system; the PDMS micro flow channel system is formed by bonding a top PDMS flow channel 700, a middle PDMS flow channel 800 and a bottom PDMS flow channel 900; the PDMS micro flow channel system is provided with a continuous phase inlet joint 100, a dispersed phase inlet joint 200, a first collection outlet joint 300, a second collection outlet joint 400, a third collection outlet joint 500 and a fourth collection outlet joint 600.
The top PDMS runner 700 includes a cross fluid transfer channel and four droplet transfer ports, wherein the cross fluid transfer channel includes a dispersed phase inlet 701, a first dispersed phase transfer port 703, a second dispersed phase transfer port 705, a third dispersed phase transfer port 707, and a fourth dispersed phase transfer port 709, and the dispersed phase inlet 701 is respectively communicated with the first dispersed phase transfer port 703, the second dispersed phase transfer port 705, the third dispersed phase transfer port 707, and the fourth dispersed phase transfer port 709 through the four branch fluid channels of the cross fluid transfer channel; the four drop delivery ports are a first drop delivery port 702, a second drop delivery port 704, a third drop delivery port 706, and a fourth drop delivery port 708, respectively, and are symmetrically disposed about the cross-shaped fluid delivery flow channel.
The middle PDMS flow channel 800 comprises four groups of T-shaped micro flow channels; the first group of T-type microchannels comprise a first continuous phase channel 804 and a first dispersed phase channel 803, the inlet end of the first continuous phase channel 804 is connected with the first continuous phase junction 801, the outlet end of the first continuous phase channel 804 is a fifth droplet delivery port 805, the middle part of the first continuous phase channel 804 is connected with the outlet end of the first dispersed phase channel 803 through the T-type microchannels, and the inlet end of the first dispersed phase channel 803 is a fifth dispersed phase delivery port 802; the second group of T-shaped micro flow channels comprises a second continuous phase flow channel 808 and a second dispersed phase flow channel 807, the inlet end of the second continuous phase flow channel 808 is connected with the first continuous phase adapter port 801, the outlet end of the second continuous phase flow channel 808 is a sixth droplet delivery port 809, the middle part of the second continuous phase flow channel 808 is connected with the outlet end of the second dispersed phase flow channel 807 through the T-shaped micro flow channel, and the inlet end of the second dispersed phase flow channel 807 is a sixth dispersed phase delivery port 806; the third group of T-shaped micro-channels comprises a third continuous phase channel 812 and a third dispersed phase channel 811, the inlet end of the third continuous phase channel 812 is connected with the first continuous phase adapter port 801, the outlet end of the third continuous phase channel 812 is a seventh droplet delivery port 813, the middle part of the third continuous phase channel 812 is connected with the outlet end of the third dispersed phase channel 811 through the T-shaped micro-channels, and the inlet end of the third dispersed phase channel 811 is a seventh dispersed phase delivery port 810; the fourth group of T-shaped microchannels comprises a fourth continuous phase flow channel 817 and a fourth dispersed phase flow channel 815, an inlet end of the fourth continuous phase flow channel 817 is connected with the first continuous phase transfer port 801, an outlet end of the fourth continuous phase flow channel 817 is an eighth droplet delivery port 816, the middle of the fourth continuous phase flow channel 817 is connected with an outlet end of the fourth dispersed phase flow channel 815 through the T-shaped microchannels, and an inlet end of the fourth dispersed phase flow channel 815 is an eighth dispersed phase delivery port 814.
The bottom PDMS flow channel 900 includes a main continuous phase flow channel 901, an inlet of the main continuous phase flow channel 901 is a main continuous phase conveying inlet 902, and a second continuous phase adapter 903 is arranged at the end of the main continuous phase flow channel 901.
The interdigital transducer 1000 comprises a piezoelectric substrate 1001, wherein an arc-shaped interdigital electrode 1002 is manufactured on the piezoelectric substrate 1001, the arc-shaped interdigital electrode 1002 comprises a plurality of pairs of interdigital, and the arc angle is 60 degrees.
The arc-shaped interdigital electrode 1002 comprises 15 pairs of interdigital fingers, and the width of each finger strip is 25 microns.
The piezoelectric substrate 1001 is made of double-sided polished 128-degree Y lithium niobate.
The arc interdigital electrode 1002 adopts a three-layer structure of chromium at the bottom layer of 50 nanometers, gold at the middle layer of 200 nanometers and silicon dioxide at the upper layer of 50 nanometers.
The height of a flow channel of the PDMS micro flow channel system is 60 micrometers, a dispersed phase inlet 701, a first droplet delivery port 702, a second droplet delivery port 704, a third droplet delivery port 706, a fourth droplet delivery port 708, a fifth droplet delivery port 805, a fifth dispersed phase delivery port 802, a sixth dispersed phase delivery port 806, a seventh dispersed phase delivery port 810, an eighth dispersed phase delivery port 814, a sixth droplet delivery port 809, a seventh droplet delivery port 813, an eighth droplet delivery port 816, a main continuous phase delivery inlet 902 and a second continuous phase transfer port 903 are all through holes, the outlets of other inlets are non-through holes, and the heights of the inlets and the outlets are as high as the height of the flow channel.
The relative position relationship among the top layer PDMS runner 700, the middle layer PDMS runner 800, the bottom layer PDMS runner 900, and the interdigital transducer 1000 is as follows: the lower surface of the top PDMS runner 700 with the runner is bonded to the upper surface of the middle PDMS runner 800 without the runner, the lower surface of the middle PDMS runner 800 with the runner is bonded to the upper surface of the bottom PDMS runner 900 without the runner, and the lower surface of the bottom PDMS runner 900 with the runner is bonded to the upper surface of the interdigital transducer 1000 with the interdigital electrode; the continuous phase inlet connector 100 is coaxially matched and communicated with the main continuous phase conveying inlet 902, and the dispersed phase inlet connector 200 is coaxially matched and communicated with the dispersed phase inlet 701; the central axes of the first dispersed phase conveying port 703 and the fifth dispersed phase conveying port 802, the second dispersed phase conveying port 705 and the sixth dispersed phase conveying port 806, the third dispersed phase conveying port 707 and the seventh dispersed phase conveying port 810, and the fourth dispersed phase conveying port 709 and the eighth dispersed phase conveying port 814 are coaxial and are communicated with each other; the central axes of the first droplet delivery port 702 and the fifth droplet delivery port 805, the central axes of the second droplet delivery port 704 and the sixth droplet delivery port 809, the central axes of the third droplet delivery port 706 and the seventh droplet delivery port 813, and the central axes of the fourth droplet delivery port 708 and the eighth droplet delivery port 816 are coaxial and are communicated with each other; the symmetry axis of the arc-shaped interdigital electrode 1002 is perpendicular to the main continuous phase flow channel 901, and the convergence center point of the arc-shaped interdigital electrode 1002 is located on one side of the main continuous phase flow channel 901 close to the arc-shaped interdigital electrode 1002.
A micro-droplet generation method of a high-flux micro-droplet generation device based on surface acoustic wave regulation comprises the following steps:
1) fixing a high-flux micro-droplet generation device regulated and controlled by surface acoustic waves on an objective table of a microscope, and ensuring that four groups of T-shaped micro-channel positions of the middle-layer PDMS channel 800 are positioned in a field of view of the microscope and are not inclined through objective observation;
2) connecting a continuous phase inlet connector 100 and a disperse phase inlet connector 200 with a continuous phase solution storage bottle and a disperse phase solution storage bottle on a nitrogen pressure injection pump respectively through Teflon guide tubes, and connecting a first collection outlet connector 300, a second collection outlet connector 400, a third collection outlet connector 500 and a fourth collection outlet connector 600 with a droplet collection container through Teflon guide tubes;
3) starting a nitrogen pressure injection pump, setting corresponding flow rates of the continuous phase inlet joint 100 and the disperse phase inlet joint 200 respectively, and stably generating micro-droplets at four groups of T-shaped micro-channels;
4) respectively connecting the positive and negative poles of the output signal of the signal generator with the two poles of the arc-shaped interdigital electrode 1002, and adjusting the output signal of the signal generator to be sine-shaped continuous output;
5) pressing the 'output' button of the signal generator, generating convergent surface acoustic waves on the interdigital transducer 1000, wherein the surface acoustic waves act on the main continuous phase flow channel 901, so that corresponding flow resistance is increased in the continuous phase and is used as an acoustic valve to regulate the flow velocity of the continuous phase, and the regulation and control of the size of liquid drops are realized.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention can overcome the defect of slow generation rate of micro-droplets in the current two-dimensional flow channel, and the device can cumulatively amplify the T-shaped micro-flow channel annular array generated by the micro-droplets, thereby realizing the generation of the micro-droplets with higher flux.
(2) The structure of each layer of flow channel is PDMS, the PDMS is very firm after being mutually bonded, the occurrence of cracking between the flow channels due to overlarge pressure of input fluid is avoided, the PDMS is transparent in whole body and easy to observe, and the bonding between the structures of the device is firm and is not easy to crack.
(3) The invention can overcome the defect that the traditional liquid drop generation method is excessively dependent on the structure and the flow velocity of the micro-channel, ensures the high-flux generation of the liquid drops, adjusts the amplitude and the frequency of the input sinusoidal voltage, and can flexibly regulate and control the generation size of the micro-liquid drops in real time.
(4) The device has smaller volume, generates liquid drops quickly and uniformly, is convenient to integrate with other devices, and realizes more complex functions.
Drawings
Fig. 1 is an isometric view of a surface acoustic wave modulated high throughput micro-droplet generation microfluidic device of the present invention.
Fig. 2 (a) is an isometric view of the top layer PDMS flow channel 700, and fig. (b) is a rear view of the top layer PDMS flow channel 700.
Fig. 3 (a) is an isometric view of the middle layer PDMS flow channel 800, and fig. (b) is a rear view of the middle layer PDMS flow channel 800.
Fig. 4 (a) is an isometric view of the bottom layer PDMS flow channel 900, and fig. (b) is a rear view of the bottom layer PDMS flow channel 900.
Fig. 5 is an isometric view of an interdigital transducer 1000.
FIG. 6 is a schematic diagram of droplet generation of a SAW regulated high throughput droplet generation apparatus.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings.
Referring to fig. 1, the acoustic surface wave regulated high-flux micro-droplet generating device comprises an interdigital transducer 1000, wherein an arc electrode is manufactured on the interdigital transducer 1000, a PDMS micro-channel system is bonded on the upper part of the interdigital transducer 1000, and the arc electrode is matched with the PDMS micro-channel system; the PDMS micro flow channel system is formed by bonding a top PDMS flow channel 700, a middle PDMS flow channel 800 and a bottom PDMS flow channel 900; the PDMS micro flow channel system is provided with a continuous phase inlet joint 100, a dispersed phase inlet joint 200, a first collection outlet joint 300, a second collection outlet joint 400, a third collection outlet joint 500 and a fourth collection outlet joint 600; the PDMS micro-channel system is used for containing dispersed phase solution and continuous phase solution samples, providing an environment for generation of micro-droplets and conveying the generated droplets to a collection outlet.
Referring to fig. 2 (a) and (b), the top PDMS flow channel 700 includes a cross-shaped fluid delivery channel and four droplet delivery ports, wherein the cross-shaped fluid delivery channel includes a dispersed phase inlet 701 and a first dispersed phase delivery port 703, a second dispersed phase delivery port 705, a third dispersed phase delivery port 707, and a fourth dispersed phase delivery port 709, and the dispersed phase inlet 701 is respectively communicated with the first dispersed phase delivery port 703, the second dispersed phase delivery port 705, the third dispersed phase delivery port 707, and the fourth dispersed phase delivery port 709 through the four branch flow channels of the cross-shaped fluid delivery channel; the four drop delivery ports are a first drop delivery port 702, a second drop delivery port 704, a third drop delivery port 706, and a fourth drop delivery port 708, respectively, and are symmetrically disposed about the cross-shaped fluid delivery flow channel.
Referring to fig. 3 (a) and (b), the middle PDMS flow channel 800 includes four sets of T-type micro flow channels; the first group of T-type microchannels comprise a first continuous phase channel 804 and a first dispersed phase channel 803, the inlet end of the first continuous phase channel 804 is connected with the first continuous phase junction 801, the outlet end of the first continuous phase channel 804 is a fifth droplet delivery port 805, the middle part of the first continuous phase channel 804 is connected with the outlet end of the first dispersed phase channel 803 through the T-type microchannels, and the inlet end of the first dispersed phase channel 803 is a fifth dispersed phase delivery port 802; the second group of T-shaped micro flow channels comprises a second continuous phase flow channel 808 and a second dispersed phase flow channel 807, the inlet end of the second continuous phase flow channel 808 is connected with the first continuous phase adapter port 801, the outlet end of the second continuous phase flow channel 808 is a sixth droplet delivery port 809, the middle part of the second continuous phase flow channel 808 is connected with the outlet end of the second dispersed phase flow channel 807 through the T-shaped micro flow channel, and the inlet end of the second dispersed phase flow channel 807 is a sixth dispersed phase delivery port 806; the third group of T-shaped micro-channels comprises a third continuous phase channel 812 and a third dispersed phase channel 811, the inlet end of the third continuous phase channel 812 is connected with the first continuous phase adapter port 801, the outlet end of the third continuous phase channel 812 is a seventh droplet delivery port 813, the middle part of the third continuous phase channel 812 is connected with the outlet end of the third dispersed phase channel 811 through the T-shaped micro-channels, and the inlet end of the third dispersed phase channel 811 is a seventh dispersed phase delivery port 810; the fourth group of T-shaped microchannels comprise a fourth continuous phase flow channel 817 and a fourth dispersed phase flow channel 815, an inlet end of the fourth continuous phase flow channel 817 is connected with the first continuous phase transfer port 801, an outlet end of the fourth continuous phase flow channel 817 is an eighth droplet delivery port 816, the middle part of the fourth continuous phase flow channel 817 is connected with an outlet end of the fourth dispersed phase flow channel 815 through the T-shaped microchannels, and an inlet end of the fourth dispersed phase flow channel 815 is an eighth dispersed phase delivery port 814; and at the four groups of T-shaped micro-channels, droplets are continuously generated by continuously shearing relative to the fluid of the dispersed phase.
Referring to fig. 4 (a) and (b), the bottom PDMS flow channel 900 includes a main continuous phase flow channel 901, an inlet of the main continuous phase flow channel 901 is a main continuous phase delivery inlet 902, and an end of the main continuous phase flow channel 901 is a second continuous phase transfer port 903.
Referring to fig. 5, the interdigital transducer 1000 includes a piezoelectric substrate 1001, an arc-shaped interdigital electrode 1002 is fabricated on the piezoelectric substrate 1001, and the arc-shaped interdigital electrode 1002 includes a plurality of pairs of interdigital, and the arc angle is 60 ° for generating a convergent surface acoustic wave on the surface of the piezoelectric substrate 1001.
The arc interdigital electrode 1002 comprises 15 pairs of interdigital fingers, the width of the finger is 25 micrometers, and surface acoustic waves with the frequency of 39.96MHz can be generated on the surface of the piezoelectric substrate 1001 under the drive of a sinusoidal alternating voltage.
The piezoelectric substrate 1001 is made of double-sided polished 128-degree Y lithium niobate.
The arc interdigital electrode 1002 is of a three-layer structure of chromium at the bottom layer of 50 nanometers, gold at the middle layer of 200 nanometers and silicon dioxide at the upper layer of 50 nanometers, wherein the chromium is used as an adhesion layer for enhancing the adhesion strength of the gold and the piezoelectric substrate 1001, the gold is used as a conductive layer, and the silicon dioxide is used as a reinforcing layer for enhancing the bonding strength of the PDMS micro flow channel system and the piezoelectric substrate 1002.
The height of a flow channel of the PDMS micro flow channel system is 60 micrometers, a dispersed phase inlet 701, a first droplet delivery port 702, a second droplet delivery port 704, a third droplet delivery port 706, a fourth droplet delivery port 708, a fifth droplet delivery port 805, a fifth dispersed phase delivery port 802, a sixth dispersed phase delivery port 806, a seventh dispersed phase delivery port 810, an eighth dispersed phase delivery port 814, a sixth droplet delivery port 809, a seventh droplet delivery port 813, an eighth droplet delivery port 816, a main continuous phase delivery inlet 902 and a second continuous phase transfer port 903 are all through holes, the outlets of other inlets are non-through holes, and the heights of the inlets and the outlets are as high as the height of the flow channel.
The relative position relationship among the top layer PDMS runner 700, the middle layer PDMS runner 800, the bottom layer PDMS runner 900, and the interdigital transducer 1000 is as follows: the lower surface of the top PDMS runner 700 with the runner is bonded to the upper surface of the middle PDMS runner 800 without the runner, the lower surface of the middle PDMS runner 800 with the runner is bonded to the upper surface of the bottom PDMS runner 900 without the runner, and the lower surface of the bottom PDMS runner 900 with the runner is bonded to the upper surface of the interdigital transducer 1000 with the interdigital electrode; the continuous phase inlet connector 100 is coaxially matched and communicated with the main continuous phase conveying inlet 902, and the dispersed phase inlet connector 200 is coaxially matched and communicated with the dispersed phase inlet 701; the central axes of the first dispersed phase conveying port 703 and the fifth dispersed phase conveying port 802, the second dispersed phase conveying port 705 and the sixth dispersed phase conveying port 806, the third dispersed phase conveying port 707 and the seventh dispersed phase conveying port 810, and the fourth dispersed phase conveying port 709 and the eighth dispersed phase conveying port 814 are coaxial and are communicated with each other; the central axes of the first droplet delivery port 702 and the fifth droplet delivery port 805, the central axes of the second droplet delivery port 704 and the sixth droplet delivery port 809, the central axes of the third droplet delivery port 706 and the seventh droplet delivery port 813, and the central axes of the fourth droplet delivery port 708 and the eighth droplet delivery port 816 are coaxial and are communicated with each other; the symmetry axis of the arc interdigital electrode 1002 is perpendicular to the main continuous phase flow channel 901, the convergence central point of the arc interdigital electrode 1002 is located on one side, close to the arc interdigital electrode 1002, of the main continuous phase flow channel 901, the convergence surface acoustic wave is generated on the interdigital transducer 1000, the surface acoustic wave acts on the main continuous phase flow channel 901, so that corresponding flow resistance is increased in the continuous phase and serves as an acoustic valve to regulate and control the flow velocity of the continuous phase, the size of the acoustic flow resistance is regulated through regulation of input voltage and frequency of the interdigital transducer 1000, regulation and control of the flow velocity of the regulated and controlled continuous phase are achieved, and the size of generated liquid drops is further regulated.
The PDMS micro-channel system is made of Polydimethylsiloxane (PDMS) with good light transmittance and biocompatibility, and optical monitoring and recording of the generation process of micro-droplets are facilitated.
A droplet generation method of a high-flux micro-droplet generation device based on surface acoustic wave regulation comprises the following steps:
1) fixing a three-dimensional microfluidic device generated by high-flux micro-droplets based on surface acoustic waves on an objective table of a microscope, and ensuring that four groups of T-shaped micro-channel positions of the middle-layer PDMS channel 800 are positioned in a field of view of the microscope and have no inclination through objective observation;
2) connecting a continuous phase inlet connector 100 and a disperse phase inlet connector 200 with a continuous phase solution storage bottle and a disperse phase solution storage bottle on a nitrogen pressure injection pump respectively through Teflon guide tubes, and connecting a first collection outlet connector 300, a second collection outlet connector 400, a third collection outlet connector 500 and a fourth collection outlet connector 600 with a droplet collection container through Teflon guide tubes;
3) starting a nitrogen pressure injection pump, setting corresponding flow rates of the continuous phase inlet joint 100 and the disperse phase inlet joint 200 respectively, and stably generating micro-droplets at four groups of T-shaped micro-channels;
4) respectively connecting the positive and negative poles of the output signal of the signal generator with the two poles of the arc-shaped interdigital electrode 1002, and adjusting the output signal of the signal generator to be sine-shaped continuous output;
5) pressing the 'output' button of the signal generator, generating convergent surface acoustic waves on the interdigital transducer 1000, wherein the surface acoustic waves act on the main continuous phase flow channel 901, so that corresponding flow resistance is increased in the continuous phase and is used as an acoustic valve to regulate the flow velocity of the continuous phase, and the regulation and control of the size of liquid drops are realized.
Referring to fig. 1, 2, 3, 4, 5 and 6, the generation process of the micro-droplets in the high-flux micro-droplet generation device based on the surface acoustic wave is as follows: the dispersed phase solution passes through the dispersed phase inlet joint 200, the dispersed phase inlet 701, the first dispersed phase delivery port 703, the second dispersed phase delivery port 705, the third dispersed phase delivery port 707, the fourth dispersed phase delivery port 709, the fifth dispersed phase delivery port 802, the sixth dispersed phase delivery port 806, the seventh dispersed phase delivery port 810 and the eighth dispersed phase delivery port 814, the dispersed phase solution simultaneously fills the first dispersed phase flow passage 803, the second dispersed phase flow passage 807, the third dispersed phase flow passage 811 and the fourth dispersed phase flow passage 815, the continuous phase solution passes through the continuous phase inlet joint 100, the main continuous phase delivery port 902, the main continuous phase flow passage 901, the second continuous phase switching port 903, the first continuous phase switching port 801 and the continuous phase solution respectively fill the first continuous phase flow passage 804, the second continuous phase flow passage 808, the third continuous phase flow passage 812 and the fourth continuous phase flow passage 816, the input pressures of the dispersed phase solution and the continuous phase solution are adjusted by a nitrogen pressure injection pump, filling the channels with the dispersed phase and the continuous phase, adjusting the dispersed phase and the continuous phase to corresponding flow velocities, continuously shearing the dispersed phase into micro-droplets at the positions of the T-shaped micro-channel structures through continuous shearing of fluid relative to the dispersed phase, realizing high-flux generation of the micro-droplets, then pressing an output button of a signal generator, generating convergent surface acoustic waves on the interdigital transducer 1000, and enabling the surface acoustic waves to act on the main continuous phase channel 901, so that corresponding flow resistance is increased in the continuous phase and the flow velocity of the continuous phase is regulated and controlled as an acoustic valve, and further the size of the droplets is regulated and controlled; then the liquid drops are conveyed to a liquid drop collecting container through the connecting ports, the conveying ports, the flow channels and the collecting outlet joint for collection.
Claims (4)
1. A surface acoustic wave regulated high-flux micro-droplet generation method is characterized in that: a high-flux micro-droplet generation device regulated by surface acoustic waves comprises an interdigital transducer (1000), wherein an arc-shaped electrode is manufactured on the interdigital transducer (1000), a PDMS micro-channel system is bonded on the upper part of the interdigital transducer (1000), and the arc-shaped electrode is matched with the PDMS micro-channel system; the PDMS micro-channel system is formed by bonding a top PDMS channel (700), a middle PDMS channel (800) and a bottom PDMS channel (900); the PDMS micro flow channel system is provided with a continuous phase inlet joint (100), a dispersed phase inlet joint (200), a first collection outlet joint (300), a second collection outlet joint (400), a third collection outlet joint (500) and a fourth collection outlet joint (600);
the top PDMS flow channel (700) comprises a cross-shaped fluid conveying flow channel and four droplet conveying ports, wherein the cross-shaped fluid conveying flow channel comprises a dispersed phase inlet (701), a first dispersed phase conveying port (703), a second dispersed phase conveying port (705), a third dispersed phase conveying port (707) and a fourth dispersed phase conveying port (709), and the dispersed phase inlet (701) is respectively communicated with the first dispersed phase conveying port (703), the second dispersed phase conveying port (705), the third dispersed phase conveying port (707) and the fourth dispersed phase conveying port (709) through the four branch flow channels of the cross-shaped fluid conveying flow channel; the four droplet delivery ports are respectively a first droplet delivery port (702), a second droplet delivery port (704), a third droplet delivery port (706) and a fourth droplet delivery port (708), and are symmetrically arranged relative to the cross-shaped fluid delivery flow channel;
the middle PDMS flow channel (800) comprises four groups of T-shaped micro flow channels; the first group of T-shaped micro-channels comprises a first continuous phase flow channel (804) and a first dispersed phase flow channel (803), the inlet end of the first continuous phase flow channel (804) is connected with a first continuous phase adapter port (801), the outlet end of the first continuous phase flow channel (804) is a fifth liquid drop conveying port (805), the middle part of the first continuous phase flow channel (804) is connected with the outlet end of the first dispersed phase flow channel (803) through the T-shaped micro-channels, and the inlet end of the first dispersed phase flow channel (803) is a fifth dispersed phase conveying port (802); the second group of T-shaped micro-channels comprises a second continuous phase channel (808) and a second dispersed phase channel (807), the inlet end of the second continuous phase channel (808) is connected with the first continuous phase adapter port (801), the outlet end of the second continuous phase channel (808) is a sixth liquid drop conveying port (809), the middle part of the second continuous phase channel (808) is connected with the outlet end of the second dispersed phase channel (807) through the T-shaped micro-channels, and the inlet end of the second dispersed phase channel (807) is a sixth dispersed phase conveying port (806); the third group of T-shaped micro-channels comprises a third continuous phase flow channel (812) and a third dispersed phase flow channel (811), the inlet end of the third continuous phase flow channel (812) is connected with the first continuous phase adapter port (801), the outlet end of the third continuous phase flow channel (812) is a seventh droplet delivery port (813), the middle part of the third continuous phase flow channel (812) is connected with the outlet end of the third dispersed phase flow channel (811) through the T-shaped micro-channels, and the inlet end of the third dispersed phase flow channel (811) is a seventh dispersed phase delivery port (810); the fourth group of T-shaped micro-channels comprises a fourth continuous phase flow channel (817) and a fourth dispersed phase flow channel (815), the inlet end of the fourth continuous phase flow channel (817) is connected with the first continuous phase adapter port (801), the outlet end of the fourth continuous phase flow channel (817) is an eighth liquid drop conveying port (816), the middle part of the fourth continuous phase flow channel (817) is connected with the outlet end of the fourth dispersed phase flow channel (815) through the T-shaped micro-channel, and the inlet end of the fourth dispersed phase flow channel (815) is an eighth dispersed phase conveying port (814);
the bottom PDMS flow channel (900) comprises a main continuous phase flow channel (901), the inlet end of the main continuous phase flow channel (901) is a main continuous phase conveying inlet (902), and the tail end of the main continuous phase flow channel (901) is a second continuous phase transfer port (903);
the interdigital transducer (1000) comprises a piezoelectric substrate (1001), wherein an arc-shaped interdigital electrode (1002) is manufactured on the piezoelectric substrate (1001), the arc-shaped interdigital electrode (1002) comprises a plurality of pairs of interdigital, and the arc angle is 60 degrees;
the relative position relation among the top PDMS runner (700), the middle PDMS runner (800), the bottom PDMS runner (900) and the interdigital transducer (1000) is as follows: the lower surface of the top PDMS runner (700) with the runner is bonded on the upper surface of the middle PDMS runner (800) without the runner, the lower surface of the middle PDMS runner (800) with the runner is bonded on the upper surface of the bottom PDMS runner (900) without the runner, and the lower surface of the bottom PDMS runner (900) with the runner is bonded on the upper surface of the interdigital transducer (1000) with the interdigital electrode; the continuous phase inlet joint (100) is coaxially matched with and communicated with the main continuous phase conveying inlet (902), and the dispersed phase inlet joint (200) is coaxially matched with and communicated with the dispersed phase inlet (701); the central axes of the first dispersed phase conveying port (703) and the fifth dispersed phase conveying port (802), the second dispersed phase conveying port (705) and the sixth dispersed phase conveying port (806), the third dispersed phase conveying port (707) and the seventh dispersed phase conveying port (810), and the fourth dispersed phase conveying port (709) and the eighth dispersed phase conveying port (814) are coaxial and are communicated with each other; the central axes of the first droplet delivery port (702) and the fifth droplet delivery port (805), the second droplet delivery port (704) and the sixth droplet delivery port (809), the third droplet delivery port (706) and the seventh droplet delivery port (813), and the fourth droplet delivery port (708) and the eighth droplet delivery port (816) are coaxial and are communicated with each other; the symmetry axis of the arc-shaped interdigital electrode (1002) is vertical to the main continuous phase flow channel (901), and the convergence central point of the arc-shaped interdigital electrode (1002) is positioned on one side of the main continuous phase flow channel (901) close to the arc-shaped interdigital electrode (1002);
the method for generating the high-flux micro-droplets regulated and controlled by the surface acoustic wave comprises the following steps:
1) fixing a three-dimensional microfluidic device generated by high-flux micro-droplets based on surface acoustic waves on an objective table of a microscope, and ensuring that four groups of T-shaped micro-channel positions of a middle-layer PDMS (polydimethylsiloxane) channel (800) are positioned in a field of view of the microscope and are not inclined through objective observation;
2) respectively connecting a continuous phase inlet connector (100) and a disperse phase inlet connector (200) with a continuous phase solution storage bottle and a disperse phase solution storage bottle on a nitrogen pressure injection pump through Teflon guide tubes, and connecting a first collection outlet connector (300), a second collection outlet connector (400), a third collection outlet connector (500) and a fourth collection outlet connector (600) with a droplet collection container through Teflon guide tubes;
3) starting a nitrogen pressure injection pump, respectively setting corresponding flow rates of a continuous phase inlet joint (100) and a dispersed phase inlet joint (200), and stably generating micro-droplets at four groups of T-shaped micro-channels;
4) respectively connecting the positive pole and the negative pole of an output signal of the signal generator with the two poles of the arc interdigital electrode (1002), and adjusting the output signal of the signal generator to be sine continuous output;
5) the output button of the signal generator is pressed, the interdigital transducer (1000) generates convergent surface acoustic waves, and the surface acoustic waves act on the main continuous phase flow channel (901), so that corresponding flow resistance is increased in the continuous phase and the flow velocity of the continuous phase is regulated and controlled by the surface acoustic waves as an acoustic valve, and the regulation and control of the size of liquid drops are realized.
2. The method for generating surface acoustic wave regulated high-flux micro-droplets as claimed in claim 1, wherein: the arc-shaped interdigital electrode (1002) comprises 15 pairs of interdigital strips, and the width of each finger strip is 25 microns.
3. The method for generating surface acoustic wave regulated high-flux micro-droplets as claimed in claim 1, wherein: the arc interdigital electrode (1002) adopts a three-layer structure of chromium at the bottom layer of 50 nanometers, gold at the middle layer of 200 nanometers and silicon dioxide at the upper layer of 50 nanometers.
4. The method for generating surface acoustic wave regulated high-flux micro-droplets as claimed in claim 1, wherein: the PDMS micro-channel system is characterized in that the heights of flow channels are all 60 micrometers, a dispersed phase inlet (701), a first droplet delivery port (702), a second droplet delivery port (704), a third droplet delivery port (706), a fourth droplet delivery port (708), a fifth droplet delivery port (805), a fifth dispersed phase delivery port (802), a sixth dispersed phase delivery port (806), a seventh dispersed phase delivery port (810), an eighth dispersed phase delivery port (814), a sixth droplet delivery port (809), a seventh droplet delivery port (813), an eighth droplet delivery port (816), a main continuous phase delivery inlet (902) and a second continuous phase transfer port (903) are all through holes, other inlets and outlets are non-through holes, and the heights of the inlets and outlets are equal to the heights of the flow channels.
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