CN110653014B - Particle multilayer film structure generating device based on surface acoustic wave - Google Patents
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Abstract
A particle multilayer film structure generating device based on surface acoustic waves comprises a piezoelectric substrate, wherein a PDMS (polydimethylsiloxane) micro-channel system is bonded on the upper part of the piezoelectric substrate, more than two arc-shaped electrodes are arranged on the piezoelectric substrate, and the arc-shaped electrodes are matched with the PDMS micro-channel system; the invention fully utilizes the advantages of the surface acoustic wave, can realize the multilayer wrapping of chemical particles and liquid drops only by applying corresponding sinusoidal voltage to different electrodes, thereby forming a multilayer film structure, has smaller volume, higher biocompatible electrode number and flow channels for amplification, and ensures that the particles are wrapped by more layers of films.
Description
Technical Field
The invention relates to the technical field of particle-wrapped microfluidics, in particular to a particle multilayer film structure generating device based on surface acoustic waves.
Background
The technology for generating the particle multilayer film structure has wide application prospect in cell screening, biological medical treatment, drug manufacturing machine transportation and the like, and the traditional method for generating the particle multilayer film structure needs manual operation of experimenters to complete multi-step cleaning and deposition, is time-consuming and labor-consuming, and is easy to cause cross contamination. Currently, the technical researches on multi-film wrapping mainly include layer-by-layer assembly (LbL), Coating methods, and multi-layer packaging (mll).
With the development of microfluidic technology, microfluidic platforms have become important tools in the fields of biology, chemistry, medicine, etc., and are gradually being researched and applied in the generation of particle layer membrane structures. At present, the particle multilayer film generation method based on the microfluidic technology mainly focuses on the following aspects: 1) designing corresponding micro-column structures in the flow channel by means of the flow channel structure design, so that the particles can pass through different fluid interfaces according to a predetermined direction, thereby realizing the envelope of the particles (Kantak C, Beyer S, YobasL, et al. A 'microfluidic pincall' for on-Chip generation of layer-by-layer-macroporous polymeric microcapsules [ J ]. Lab on A Chip,2011,11(6):1030-1035.Matosevics, Paegel B M. Stepwise Synthesis of Giant Unilamellar particles a microfluidic Assembly Line [ J ]. Journal of the American Chemical Society,2011,133 (2809): 2798.); 2) the coating of particles is achieved by means of fluid inertia and interfaces (Gossett D R, Tse HT, Dudani J S, et al. inert management and transfer of particulate gases laminar flow [ J ]. Small,2012,8(16): 2757-; 3) the particles are enveloped across different fluidic interfaces by an external driving force (Moon B U, Hakimi N, Hwang D K, oral. microfluidic coherent coating of non-biological magnetic particles [ J ]. Biomicrofluidics,2013,8(5): 363). However, in the above methods, the first two methods generally only can wrap the particle monolayer film, and have a single structure and poor flexibility; the reported external force driving generation method at present depends on magnetic field force to carry out single-film wrapping on magnetic particles, and the method only needs to carry out magnetic modification on the particles, so that the application is limited.
Disclosure of Invention
In order to overcome the disadvantages of the prior art, the present invention provides a device for generating a multilayer film structure of particles based on surface acoustic waves, which fully utilizes the advantages of surface acoustic waves, can realize multilayer coating of chemical particles (PS microspheres) and droplets by applying corresponding sinusoidal voltages to different electrodes, thereby forming a multilayer film structure, and has a small volume, a high number of electrodes with biocompatibility, and an expandable flow channel, thereby allowing the particles to be coated with more layers of films.
In order to achieve the purpose, the invention adopts the following technical scheme:
a particle multilayer film structure generating device based on surface acoustic waves comprises a piezoelectric substrate 1, wherein more than two arc-shaped electrodes are manufactured on the surface of the piezoelectric substrate 1, a PDMS micro-channel system is bonded on the upper part of the piezoelectric substrate 1, and the arc-shaped electrodes are matched with the PDMS micro-channel system;
the PDMS micro flow channel system comprises a first inlet flow channel 5, a second inlet flow channel 7 and a third inlet flow channel 9, wherein the inlet end of the first inlet flow channel 5 is connected with a sheath liquid flow inlet joint 4, and the outlet end of the first inlet flow channel 5 is connected with the inlet end of a particle coating flow channel 10; the inlet end of the second inlet flow channel 7 is connected with the water phase inlet joint 6, and the outlet end of the second inlet flow channel 7 is connected with the inlet end of the particle coating flow channel 10; the inlet end of a third inlet flow channel 9 is connected with an oil phase inlet joint 8, and the outlet end of the third inlet flow channel 9 is connected with the inlet end of a particle coating flow channel 10;
the outlet end of the particle-coated flow channel 10 is connected with the inlet end of a first outlet flow channel 12, and the outlet end of the first outlet flow channel 12 is connected with an oil phase outlet joint 13; the outlet end of the particle-coated flow channel 10 is connected with the inlet end of a second outlet flow channel 15, and the outlet end of the second outlet flow channel 15 is connected with a water phase outlet joint 14; the outlet end of the particle-coated flow channel 10 is connected to the inlet end of the third outlet flow channel 2, and the outlet end of the third outlet flow channel 2 is connected to the sheath fluid outlet connector 16.
The arc electrode include first arc electrode 3 and second arc electrode 11, the relative position between first arc electrode 3, first arc electrode 11 and the PDMS microchannel system does: in the horizontal direction, the distance between the symmetry axes of the first arc-shaped electrode 3 and the second arc-shaped electrode 11 is 600 um; in the vertical direction, the first arc-shaped electrode 3 and the second arc-shaped electrode 11 are located on both sides of the particle-packed flow channel 10.
The first arc-shaped electrode 3 and the second arc-shaped electrode 11 both comprise a plurality of pairs of interdigital fingers, and the arc angle is 60 degrees.
The first arc-shaped electrode 3 and the second arc-shaped electrode 11 both comprise 14 pairs of interdigital fingers, the width of the interdigital fingers is 25 micrometers, and the arc angle is 60 degrees.
The height of the flow channel in the PDMS micro flow channel system is 80 micrometers, the width values of different parts of the flow channel are different, and the width of the first inlet flow channel 5, the width of the third inlet flow channel 9, the width of the first outlet flow channel 12 and the width of the third outlet flow channel 2 are 100 micrometers and are arc-shaped flow channels; the width of the second inlet flow channel 7 and the second outlet flow channel 15 is 100um, and is a direct flow channel.
The piezoelectric substrate 1 is made of double-sided polished 128-degree Y lithium niobate.
The first arc-shaped electrode 3 and the second arc-shaped electrode 11 adopt a double-layer structure of 50nm bottom chromium and 200nm upper gold.
A method for using a surface acoustic wave-based particle multilayer film structure generation device comprises the following steps:
1) fixing the particle multilayer film structure generating device on an objective table of a microscope, and ensuring that the boundary of a particle-wrapped flow channel 10 in the PDMS micro-flow channel system is positioned in the field of view of the microscope and has no inclination through objective observation;
2) the sheath liquid flow inlet joint 4, the water phase inlet joint 6 and the oil phase inlet joint 8 are respectively connected with a sheath liquid flow solution storage bottle, a water phase solution storage bottle and an oil phase solution storage bottle on the nitrogen pressure injection pump through Teflon catheters, and the oil phase outlet joint 13, the water phase outlet joint 14 and the sheath liquid flow outlet joint 16 are connected with an oil phase waste liquid collecting container, a water phase waste liquid collecting container and a multi-membrane coated particle collecting container through Teflon catheters;
3) respectively connecting the positive and negative poles of output signals of the two signal generators with the two poles of a first arc electrode 3 and a second arc electrode 11 of the particle multilayer film structure generating device, and adjusting the output signals of the signal generators to be sine continuous output, wherein the frequency is 39.96MHz, and the voltage amplitude is 25-40 Vpp;
4) and starting the injection pump, forming stable laminar flows of the sheath liquid flow, the water phase and the oil phase of the suspended particles by adjusting the input pressure of the sheath liquid flow inlet joint 4, the water phase inlet joint 6 and the oil phase inlet joint 8, and pressing an output button of a signal generator after the three-phase fluid forms a stable water-oil interface A in the particle wrapping flow channel 10 to generate the particle multilayer film structure.
Compared with the prior art, the invention has the following beneficial effects:
(1) the invention can overcome the defects that the traditional particle multi-membrane wrapping depends on manual work, needs multi-step cleaning and is easy to cause cross contamination, only sine voltage is applied through different arc electrodes, and the generation of particle single-layer to multi-membrane structures can be realized.
(2) The method can be suitable for the generation of multilayer films on the surfaces of any solid particles or liquid drops without modifying the particles.
(3) The invention is distributed with a plurality of groups of arc electrode bodies, and can realize the wrapping generation of more films.
(4) The method has the characteristic of strong flexibility, and can open and close corresponding electrodes according to actual needs to realize the generation of the required film.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
Fig. 2 is a schematic diagram illustrating a principle of generating a particle multilayer film according to an embodiment of the present invention.
Detailed Description
The present invention will be described in detail with reference to the accompanying drawings and examples.
Referring to fig. 1, a device for generating a particle multilayer film structure based on surface acoustic waves comprises a piezoelectric substrate 1, wherein two arc-shaped electrodes are manufactured on the surface of the piezoelectric substrate 1, a PDMS micro-channel system is bonded on the upper part of the piezoelectric substrate 1, and the arc-shaped electrodes are matched with the PDMS micro-channel system;
the PDMS micro flow channel system comprises a first inlet flow channel 5, a second inlet flow channel 7 and a third inlet flow channel 9, wherein the inlet end of the first inlet flow channel 5 is connected with a sheath liquid flow inlet joint 4, and the outlet end of the first inlet flow channel 5 is connected with the inlet end of a particle coating flow channel 10; the inlet end of the second inlet flow channel 7 is connected with the water phase inlet joint 6, and the outlet end of the second inlet flow channel 7 is connected with the inlet end of the particle coating flow channel 10; the inlet end of a third inlet flow channel 9 is connected with an oil phase inlet joint 8, and the outlet end of the third inlet flow channel 9 is connected with the inlet end of a particle coating flow channel 10;
the outlet end of the particle-coated flow channel 10 is connected with the inlet end of a first outlet flow channel 12, and the outlet end of the first outlet flow channel 12 is connected with an oil phase outlet joint 13; the outlet end of the particle-coated flow channel 10 is connected with the inlet end of a second outlet flow channel 15, and the outlet end of the second outlet flow channel 15 is connected with a water phase outlet joint 14; the outlet end of the particle-coated flow channel 10 is connected to the inlet end of the third outlet flow channel 2, and the outlet end of the third outlet flow channel 2 is connected to the sheath fluid outlet connector 16.
The first inlet flow channel 5, the second inlet flow channel 7 and the third inlet flow channel 9 are respectively introduced with a sheath fluid, a water phase containing a particle sample and an oil phase containing phospholipid, and the three-phase fluid forms a stable water-oil interface A in the particle coating flow channel 10, as shown in FIG. 2; the oil phase outlet joint 13, the water phase outlet joint 14 and the sheath liquid outlet joint 16 are used for collecting oil phase waste liquid, water phase waste liquid and collecting multi-membrane coated particles.
The arc electrode include first arc electrode 3 and second arc electrode 11, the relative position between first arc electrode 3, first arc electrode 11 and the PDMS microchannel system does: in the horizontal direction, the distance between the symmetry axes of the first arc-shaped electrode 3 and the second arc-shaped electrode 11 is 600 um; in the vertical direction, the first arc-shaped electrode 3 and the second arc-shaped electrode 11 are positioned at two sides of the particle-packed flow channel 10; the convergence center of the first arc-shaped electrode 3 is 10um away from the upper boundary of the particle-coated flow channel 10, and the convergence center of the second arc-shaped electrode 11 is 10um away from the upper boundary of the particle-coated flow channel 10; the first arc-shaped electrode 3 and the second arc-shaped electrode 11 are distributed at two sides of the flow channel in a staggered manner to generate reverse surface acoustic waves, so that particles generate deflection motion deviating from the direction of the arc-shaped electrodes in the flow channel, and power is provided for realizing multi-film wrapping of the particles; in addition, under the condition of ensuring that the PDMS micro-channel system has no leakage, the width of the PDMS micro-channel close to one side of the first arc-shaped electrode 3 and one side of the second arc-shaped electrode 11 should be reduced as much as possible, so that the absorption of the PDMS on the sound wave energy can be reduced, and the utilization efficiency of the gathered sound wave energy can be improved.
The first arc-shaped electrode 3 and the second arc-shaped electrode 11 both comprise a plurality of pairs of interdigital fingers, the arc angle is 60 degrees, and the first arc-shaped electrode and the second arc-shaped electrode are used for generating convergent surface acoustic waves on the surface of the piezoelectric substrate 1, and the convergent surface acoustic waves mainly act on particles in a water phase.
The first arc-shaped electrode 3 and the second arc-shaped electrode 11 both comprise 14 pairs of interdigital fingers, the width of the interdigital fingers is 25 micrometers, the arc angle is 60 degrees, and based on the piezoelectric effect, surface acoustic waves with the frequency of 39.96MHz can be generated on the surface of the piezoelectric substrate 1 under the drive of sinusoidal alternating voltage.
The height of each micro-channel is 80 microns, the width values of different parts of each micro-channel are different, and the width of each of the first inlet channel 5, the third inlet channel 9, the first outlet channel 12 and the third outlet channel 2 is 100 microns and is an arc-shaped channel; the width of the second inlet flow channel 7 and the second outlet flow channel 15 is 100um, and is a direct flow channel.
The piezoelectric substrate 1 is made of double-sided polished 128-degree Y-cut lithium niobate (128-degree Y-cut LiNbO 3).
The first arc-shaped electrode 3 and the second arc-shaped electrode 11 adopt a double-layer structure of 50nm bottom-layer chromium and 200nm top-layer gold, wherein the chromium is used as an adhesion layer for enhancing the adhesion strength of the gold and the piezoelectric substrate 1, and the gold is used as a conductive layer.
The PDMS micro-channel system is made of Polydimethylsiloxane (PDMS) with good light transmittance and biocompatibility, and optical monitoring and recording are conveniently carried out in the process of forming the multi-membrane structure.
A method for using a surface acoustic wave-based particle multilayer film structure generation device comprises the following steps:
1) fixing the particle multilayer film structure generating device on an objective table of a microscope, and ensuring that the boundary of a particle-wrapped flow channel 10 in the PDMS micro-flow channel system is positioned in the field of view of the microscope and has no inclination through objective observation;
2) the sheath liquid flow inlet joint 4, the water phase inlet joint 6 and the oil phase inlet joint 8 are respectively connected with a sheath liquid flow solution storage bottle, a water phase solution storage bottle and an oil phase solution storage bottle on the nitrogen pressure injection pump through Teflon catheters, and the oil phase outlet joint 13, the water phase outlet joint 14 and the sheath liquid flow outlet joint 16 are connected with an oil phase waste liquid collecting container, a water phase waste liquid collecting container and a multi-membrane coated particle collecting container through Teflon catheters;
3) respectively connecting the positive and negative poles of output signals of the two signal generators with the two poles of a first arc electrode 3 and a second arc electrode 11 of the particle multilayer film structure generating device, and adjusting the output signals of the signal generators to be sine continuous output, wherein the frequency is 39.96MHz, and the voltage amplitude is 25-40 Vpp; the first arc-shaped electrode 3 generates convergent surface acoustic waves with convergent energy beams, under the action of acoustic radiation force, particles in the particle coating channel are deflected and pass through an oil-water interface to enter an oil phase, and a layer of water film is coated on the surface; then when the particles coated with the water film flow to the second arc-shaped electrode 11 along with the oil phase, the second arc-shaped electrode 11 generates convergent surface acoustic waves to enable the particles coated with the water film to pass through an oil-water interface from the oil phase and enter a sheath liquid phase, and the coating of the second layer of oil film is realized;
4) and starting the injection pump, forming stable laminar flows of the sheath liquid flow, the water phase and the oil phase of the suspended particles by adjusting the input pressure of the sheath liquid flow inlet joint 4, the water phase inlet joint 6 and the oil phase inlet joint 8, and pressing an output button of a signal generator after the three-phase fluid forms a stable water-oil interface A in the particle wrapping flow channel 10 to generate the particle multilayer film structure.
Referring to fig. 2, the generation process of the particle multi-membrane structure in the PDMS micro-channel system is as follows: the sheath fluid solution, the water phase solution and the oil phase solution simultaneously enter a first inlet flow channel 5, a second inlet flow channel 7 and a third inlet flow channel 9 respectively, the input pressure of the sheath fluid solution, the water phase solution and the oil phase solution is adjusted by a nitrogen pressure injection pump, so that the sheath fluid solution, the water phase solution and the oil phase solution are filled in a particle wrapping flow channel 10 in a laminar flow mode, and a stable water-oil interface A shown by a dotted line in figure 2 is formed; when sine alternating voltage is input to the first arc-shaped electrode 3, convergent surface acoustic waves with convergent energy beams are generated, under the action of acoustic radiation force, particles in the particle coating channel 10 are deflected and pass through an oil-water interface to enter an oil phase, and a layer of water film is coated on the surface; then when the particles coating the water film flow to the second arc electrode 11 along with the oil phase, the second arc electrode 11 generates convergent surface acoustic waves, and the particles coating the water film pass through a water-oil interface A from the oil phase to enter a sheath liquid phase under the action of acoustic radiation force, so that the coating of the second layer of oil film is realized; the amplitude and frequency parameters of the input voltage of the sinusoidal alternating voltage of the second arc electrode 11 are adjusted, and when the generated acoustic radiation force is large enough, the particles with two layers of membranes can be deflected to the sheath liquid phase for collection.
Claims (6)
1. A method for using a particle multilayer film structure generating device based on surface acoustic waves is characterized in that: a particle multilayer film structure generating device based on surface acoustic waves comprises a piezoelectric substrate (1), wherein more than two arc-shaped electrodes are manufactured on the surface of the piezoelectric substrate (1), a PDMS micro-channel system is bonded on the upper part of the piezoelectric substrate (1), and the arc-shaped electrodes are matched with the PDMS micro-channel system;
the PDMS micro flow channel system comprises a first inlet flow channel (5), a second inlet flow channel (7), a third inlet flow channel (9), a first outlet flow channel (12), a second outlet flow channel (15), a third outlet flow channel (2) and a particle coating flow channel (10); the inlet end of the first inlet flow channel (5) is connected with the sheath liquid flow inlet joint (4), the outlet end of the first inlet flow channel (5) is connected with the particle-coated flow channel (10), the inlet end of the second inlet flow channel (7) is connected with the water phase inlet joint (6), the outlet end of the second inlet flow channel (7) is connected with the particle-coated flow channel (10), the inlet end of the third inlet flow channel (9) is connected with the oil phase inlet joint (8), and the outlet end of the third inlet flow channel (9) is connected with the inlet end of the particle-coated flow channel (10);
the outlet end of the particle-coated flow passage (10) is connected with the inlet end of a first outlet flow passage (12), and the outlet end of the first outlet flow passage (12) is connected with an oil phase outlet joint (13); the outlet end of the particle-coated flow channel (10) is connected with the inlet end of a second outlet flow channel (15), and the outlet end of the second outlet flow channel (15) is connected with a water phase outlet joint (14); the outlet end of the particle coating flow channel (10) is connected with the inlet end of a third outlet flow channel (2), and the outlet end of the third outlet flow channel (2) is connected with a sheath liquid outlet joint (16);
the arc electrode include first arc electrode (3) and second arc electrode (11), relative position between first arc electrode (3), second arc electrode (11) and the PDMS miniflow channel system does: in the horizontal direction, the symmetry axes of the first arc-shaped electrode (3) and the second arc-shaped electrode (11) are 600 microns away; in the vertical direction, the first arc-shaped electrode (3) and the second arc-shaped electrode (11) are positioned at two sides of the particle-coated flow channel (10);
the use method of the surface acoustic wave-based particle multilayer film structure generation device comprises the following steps:
1) fixing the particle multilayer film structure generating device on an objective table of a microscope, and ensuring that the boundary of a particle-wrapped flow channel (10) in the PDMS micro-flow channel system is positioned in the field of view of the microscope and has no inclination through objective observation;
2) the sheath liquid flow inlet joint (4), the water phase inlet joint (6) and the oil phase inlet joint (8) are respectively connected with a sheath liquid flow solution storage bottle, a water phase solution storage bottle and an oil phase solution storage bottle on the nitrogen pressure injection pump through Teflon catheters, and the oil phase outlet joint (13), the water phase outlet joint (14) and the sheath liquid flow outlet joint (16) are connected with an oil phase waste liquid collecting container, a water phase waste liquid collecting container and a multi-membrane coated particle collecting container through Teflon catheters;
3) respectively connecting the positive and negative poles of output signals of the two signal generators with the two poles of a first arc electrode (3) and a second arc electrode (11) of the particle multilayer film structure generating device, and adjusting the output signals of the signal generators to be sine continuous output, wherein the frequency is 39.96MHz, and the voltage amplitude is 25-40 Vpp;
4) and starting a syringe pump, forming stable laminar flows of the sheath liquid flow, the water phase and the oil phase of the suspended particles by adjusting the input pressure of the sheath liquid flow inlet joint (4), the water phase inlet joint (6) and the oil phase inlet joint (8), and pressing an output button of a signal generator to generate a particle multilayer film structure after the three-phase fluid forms a stable water-oil interface (A) in the particle wrapping flow channel (10).
2. The method of using a surface acoustic wave-based particle multilayer film structure generating apparatus according to claim 1, wherein: the first arc-shaped electrode (3) and the second arc-shaped electrode (11) respectively comprise a plurality of pairs of interdigital fingers, and the arc angle is 60 degrees.
3. The method of using a surface acoustic wave-based particle multilayer film structure generating apparatus according to claim 1, wherein: the first arc-shaped electrode (3) and the second arc-shaped electrode (11) respectively comprise 14 pairs of interdigital fingers, the width of each finger is 25 micrometers, and the arc angle is 60 degrees.
4. The method of using a surface acoustic wave-based particle multilayer film structure generating apparatus according to claim 1, wherein: the height of each micro-channel is 80 microns, the width values of different parts of each micro-channel are different, and the width of each of the first inlet channel (5), the third inlet channel (9), the first outlet channel (12) and the third outlet channel (2) is 100 microns and is an arc-shaped channel; the width of the second inlet flow channel (7) and the second outlet flow channel (15) is 100 microns, and the second inlet flow channel and the second outlet flow channel are straight flow channels.
5. The method of using a surface acoustic wave-based particle multilayer film structure generating apparatus according to claim 1, wherein: the piezoelectric substrate (1) is made of double-sided polished 128-degree Y lithium niobate.
6. The method of using a surface acoustic wave-based particle multilayer film structure generating apparatus according to claim 1, wherein: the first arc-shaped electrode (3) and the second arc-shaped electrode (11) adopt a double-layer structure of 50nm bottom chromium and 200nm upper gold.
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