CN111084382B - Instant plant-based emulsion special for inflammatory bowel diseases and preparation method thereof - Google Patents
Instant plant-based emulsion special for inflammatory bowel diseases and preparation method thereof Download PDFInfo
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- CN111084382B CN111084382B CN202010040183.5A CN202010040183A CN111084382B CN 111084382 B CN111084382 B CN 111084382B CN 202010040183 A CN202010040183 A CN 202010040183A CN 111084382 B CN111084382 B CN 111084382B
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
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- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A23L7/00—Cereal-derived products; Malt products; Preparation or treatment thereof
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- A23L7/25—Fermentation of cereal malt or of cereal by malting
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
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Abstract
The invention discloses a special instant plant-based emulsion for inflammatory bowel diseases and a preparation method thereof, which comprises the steps of germinating and fermenting whole grains, concentrating, drying, grinding, crushing to prepare germinated and fermented grain superfine powder, mixing with spirulina polysaccharide, aureobasidium polysaccharide and soybean protein peptide, adding water, and carrying out colloid milling to obtain grain mixed slurry; adding emulsifier into the compound vegetable oil to obtain emulsion oil phase component; mixing the grain mixed pulp with the emulsion oil phase components, adding compound mineral, compound vitamin, and pericarpium Granati extract, stirring, grinding with colloid mill, homogenizing under high pressure, sterilizing, and packaging. The invention combines N-3 and N-6 unsaturated fatty acid, strengthens the immunity of organisms and anti-inflammatory action, carries out germination and fermentation treatment on whole grain food, has easy absorption of nutrition, increases the quantity of beneficial bacteria in intestinal flora, reduces the proportion of pathogenic bacteria, reduces the level of intestinal tract and systemic inflammatory factors, is an ideal substitute for animal-derived food, and meets the special nutritional requirements of patients with inflammatory bowel diseases.
Description
Technical Field
The invention belongs to the technical field of food processing, and particularly relates to a special instant plant-based emulsion for inflammatory bowel diseases and a preparation method thereof.
Background
Inflammatory Bowel Disease (IBD) is enteritis and colitis caused by bacteria, viruses, fungi, and parasites. Is a serious gastrointestinal disease, is difficult to cure and easy to relapse, and has the conditions of continuously rising morbidity, gradually aging onset age and the like. The main pathological mechanism of inflammatory bowel disease is not well defined, and the pathogenesis of inflammatory bowel disease is multifaceted, such as genetic factors and environmental factors, which can cause intestinal innate and adaptive mucosal immune response.
In recent years, plant-based products have become increasingly popular with consumers. Especially whole grain food, its probiotic effect is more and more appreciated. Although the pathogenesis of IBD is still unclear, in recent years, as related studies are continuously carried out, intestinal flora imbalance is prevalent among IBD patients and is considered to be a main cause of IBD. Researches show that intestinal bacteria disorder can cause abnormal immune response to cause inflammatory mucosal injury of colon tissues; and improving intestinal flora can effectively regulate the intestinal mucosa immune system, relieve inflammatory injury of the intestinal tract and enhance the barrier function of the intestinal tract. Therefore, by ingesting prebiotics, the symptoms of IBD patients are improved, and this can be an important means for the adjuvant dietary therapy of inflammatory bowel diseases.
Although the whole grain contains a large amount of prebiotics, which is beneficial to adjusting and optimizing the intestinal flora structure of animals and human bodies, the bran of the whole grain contains anti-nutrient substances such as phytic acid, which can increase the intestinal burden of IBD patients and aggravate diarrhea. Although our country has achieved some achievements in the research of whole grains, on the whole, there are relatively few varieties, and there are not many products that can really reach the production scale. Therefore, the development of medical products related to inflammatory bowel disease by using whole grains needs to reasonably optimize the formula and the preparation process.
Compared with the traditional powder product-shaped instant food, the emulsion product has the characteristic of instant uncovering and is more convenient to use. Based on the whole grain rich in prebiotics, the emulsion product with stable quality and meeting the special nutritional requirements of inflammatory bowel diseases is developed, can fill the blank of related product forms in China at present, and meets the nutritional support requirements of a large number of patients with inflammatory bowel diseases.
Disclosure of Invention
Aiming at the problem of the prior art that special food and related emulsifier products specially for patients with inflammatory bowel diseases are deficient, the invention provides the special instant plant-based emulsion for the inflammatory bowel diseases and the preparation method thereof.
The invention is realized by the following technical scheme:
the special instant plant-based emulsion for inflammatory bowel diseases is characterized by comprising the following raw materials in parts by weight: 20-50 parts of whole grain germinated fermentation ultramicro powder, 10-30 parts of compound vegetable oil and 0.5-5 parts of emulsifier.
Furthermore, the raw materials also comprise 10-30 parts of soybean protein peptide, 10-30 parts of spirulina polysaccharide, 5-20 parts of pomegranate peel polyphenol extract, 1-10 parts of aureobasidium polysaccharide and 0.5-1 part of stabilizer.
Furthermore, the raw materials also comprise more than one of compound mineral substances, compound vitamins and flavoring agents.
Further, the whole grain is more than one of quinoa, millet and rice.
Furthermore, the grain diameter of the whole grain submicron powder is 18-20 μm.
Further, the compound vegetable oil is a mixture of A and B according to a mass ratio of 1; the group A vegetable oil is more than one of soybean oil, peanut oil and rapeseed oil; the group B vegetable oil is more than one of chia seed oil, linseed oil, olive oil and walnut oil.
Among unsaturated fatty acids (PUFA), N-3 unsaturated fatty acids and N-6 unsaturated fatty acids play an important role in human bodies, wherein the N-6 unsaturated fatty acids are rich in sources (soybean oil, peanut oil and rapeseed oil) and low in price, but have a certain promotion effect on body immunity and enhance inflammatory reaction, the N-3 unsaturated fatty acids (chia seed oil, linseed oil, olive oil and walnut oil) have an immunosuppressive effect and reduce inflammatory reaction, and the two unsaturated fatty acids can act together to regulate the viscosity of blood and prevent bleeding or blood coagulation at inflammatory parts.
Further, the emulsifier is at least two of lecithin, sucrose fatty acid ester, monoglyceride and capric triglyceride; the plant-based emulsion is a natural grain raw material, belongs to a complex food system, is compounded by adopting a plurality of emulsifiers, can adjust the HLB of the composite emulsifier, and can better match various emulsified objects in the system.
Further, the stabilizer is at least one of xanthan gum, konjac gum and guar gum.
In the invention, the preparation method of the instant plant-based emulsion special for inflammatory bowel disease comprises the following steps:
(1) Soaking and cleaning whole grains, spreading on a soaked cotton cloth, germinating at 20-28 ℃ for 12-24 hours, drying at 40-55 ℃ for 12-24 hours, grinding to obtain germinated whole grain powder, adding the mixture into a container, wherein the weight ratio of the germinated whole grain powder to the grain raw materials is 1: steaming for 5-15 minutes by using water of 1-3, cooling to room temperature, adding a leavening agent, uniformly stirring, fermenting for 24-48 hours at 37 ℃ to obtain germinated fermented grain slurry, concentrating, drying, grinding, and performing superfine grinding to prepare germinated fermented grain superfine powder with the grain size of 18-20 microns;
(2) Mixing the germinated and fermented grain superfine powder with spirulina polysaccharide, aureobasidium polysaccharide and soybean protein peptide, adding water according to the material-liquid ratio of 1;
(3) Keeping the temperature of the compound vegetable oil at 55-65 ℃ for half an hour, adding the water-soluble emulsifier, continuously stirring for 10-20min to obtain an emulsion oil phase component, and keeping the temperature at 55-65 ℃ for later use;
(4) Heating the grain mixed slurry prepared in the step (2) to 55-65 ℃, adding water and a stabilizer with the volume of 3-6 times, stirring for 15-20min, adding the emulsion oil phase component prepared in the step (3), keeping the temperature at 55-65 ℃, stirring for 3-5 min, adding the compound mineral, the compound vitamin and the pomegranate peel extract, stirring and uniformly mixing for 5-10min at the temperature of 55-65 ℃, grinding for 2-3 times by using a colloid mill, homogenizing for 2-3 times at the high pressure of 25-35MPa by using a high-pressure homogenizer, sterilizing and packaging to obtain the instant plant-based emulsion special for the inflammatory bowel disease.
Further, the fermentation inoculum in step (1) is lactobacillus plantarum powder, and the inoculation concentration is 10 per 1L of grain pulp 5 -10 7 cfu。
Whole grain refers to whole, crushed, broken or tabletted grain, which basically consists of starchy endosperm, germ and cortex, and contains not only abundant B-group vitamins, magnesium, iron and dietary fibers, but also common antioxidant ingredients such as polyphenol, vitamin E, tannin, carotenoid, phytic acid, lignin and lignan, and also antioxidant ingredients which are rare in some fruit and vegetable foods but have high nutritional values, such as gamma-oryzanol, alkylresorcinol, avenanthramide, and the like. The sprouted grain is a novel functional food, which not only contains various bioactive components such as gamma-aminobutyric acid and glutathione, but also is easy to digest and absorb by human bodies after the sprouted grain, and not only generates a plurality of physiological metabolic changes such as activation and generation of enzyme and recovery of cell physiological activity in the process of the sprouted grain, but also can improve the nutritional quality of the grain, such as improving the composition of amino acid, improving the utilization rate of protein, increasing the content of B vitamins, reducing anti-nutritional factors and the like. In the later stage, a large amount of beneficial secondary metabolites are generated in the lactobacillus plantarum fermentation process, so that the flora regulating function of the whole grain food is further enhanced. Compared with non-whole grain or non-germinated and fermented whole grain food, the germinated and fermented specific grain food can effectively increase the number of beneficial bacteria in intestinal flora and reduce the proportion of conditional pathogenic bacteria in the flora, so that the levels of intestinal tract and systemic inflammatory factors are reduced, and the effect of preventing or assisting in treating inflammatory bowel diseases is achieved. Although China has achieved some achievements in the research of the sprouted grains, in general, the sprouted grains have relatively few varieties, and products which can really reach the production scale are not many.
Advantageous effects
(1) According to the plant-based raw materials adopted in the invention, the N-3 unsaturated fatty acid and the N-6 unsaturated fatty acid are used in a matching manner, so that the immunity of the organism is enhanced, the anti-inflammatory effect is enhanced, the viscosity of blood in an intestinal tract is adjusted, and bleeding or blood coagulation at an inflammation part is prevented;
(2) The invention carries out germination and fermentation treatment on the full-grain food with rich nutrition, the nutrient components are easy to be digested and absorbed by human bodies, the specific grain food after germination and fermentation can effectively increase the number of beneficial bacteria in intestinal flora and reduce the proportion of conditional pathogenic bacteria in the flora, thereby reducing the level of intestinal tract and systemic inflammatory factors, playing a role in preventing or assisting in treating inflammatory bowel diseases, maintaining the epithelial structure of the small intestine, being an ideal substitute for animal-derived food, and completely meeting the special nutritional requirements of patients with inflammatory bowel diseases.
Drawings
FIG. 1 shows the body weight change of mice during induction;
FIG. 2 is the change in Disease Activity Index (DAI) in mice during induction;
FIG. 3 is a graph showing the results of pathological staining of colon tissues of the mouse in example 1;
FIG. 4 is a graph showing the results of pathological staining of colon tissues of a mouse in comparative example 1;
FIG. 5 is a graph showing the results of pathological staining of colon tissues of mice in comparative example 2;
FIG. 6 is a graph showing the result of pathological staining of colon tissue in a mouse of comparative example 3;
FIG. 7 is a graph showing the result of pathological staining of colon tissue in a mouse of comparative example 4;
FIG. 8 is a graph showing the results of pathological staining of colon tissues of mice in comparative example 5;
FIG. 9 is a change in the phylum Proteobacteria (Proteobacteria) of the intestinal tract of mice during induction;
FIG. 10 shows the variation of the pathogenic bacterium Shigella coli (Escherichia-Shigella) in the mouse intestinal tract during induction;
FIG. 11 shows the variation of the beneficial bacterium Lactobacillus (Lactobacillus) in the mouse intestinal tract during induction;
FIG. 12 shows the change of Bifidobacterium (beneficial bacterium) in the mouse intestinal tract during induction.
Detailed Description
For better understanding of the present invention, the technical solution of the present invention will be described in detail with specific examples, but the present invention is not limited thereto.
The compound vitamin used in the embodiment of the invention is compound vitamin powder, comprising vitamin C, vitamin A, vitamin D2, vitamin B11, riboflavin, vitamin B6, nicotinamide, dexpanthenol, vitamin E, folic acid, biotin and vitamin B12; the compound mineral comprises calcium, potassium, magnesium, ferrum, copper, zinc, manganese, iodine, selenium, nickel, etc.
The parts in the examples of the present invention are parts by weight.
Example 1
The raw materials of the instant plant-based emulsion special for inflammatory bowel diseases comprise: 30 parts of whole-grain superfine powder, 10 parts of compound vegetable oil (soybean oil, 10 parts of linseed oil), 20 parts of soybean protein peptide, 20 parts of spirulina polysaccharide, 10 parts of pomegranate bark polyphenol extract, 8 parts of aureobasidium polysaccharide, 2 parts of emulsifier (lecithin and 1 part of sucrose fatty acid ester), 0.4 part of stabilizer (xanthan gum and 0.4 part of konjac glucomannan), 3 parts of compound vitamin, 10 parts of compound mineral substance and 0.5 part of essence.
The preparation method of the instant plant-based emulsion special for inflammatory bowel diseases comprises the following steps:
(1) Soaking and cleaning millet, spreading on soaked cotton cloth, germinating for 20 hours at 25 ℃, drying for 20 hours at 50 ℃, grinding to obtain germinated whole millet powder, and adding the components in an amount which is 1:3, steaming for 10 minutes, cooling to room temperature, and adding 5 × 10 6 Uniformly stirring cfu lactobacillus plantarum powder, fermenting for 36 hours at 37 ℃ to obtain germinated fermented millet pulp, concentrating, drying, grinding, and performing superfine grinding treatment on the germinated fermented millet powder by using a superfine grinder to prepare germinated fermented millet superfine powder with the particle size of 18-20 microns;
(2) Mixing the germinated and fermented millet superfine powder with spirulina polysaccharide, aureobasidium polysaccharide and soybean protein peptide, adding water according to a material-liquid ratio of 1;
(3) Keeping the temperature of the compound vegetable oil at 60 ℃ for half an hour, adding a water-soluble emulsifier (the emulsifier can be dissolved just by adding water), continuously stirring for 10-20min to obtain an emulsion oil phase component, and keeping the temperature at 60 ℃ for later use;
(4) Adding 3 times of water into the millet mixed slurry prepared in the step (2), heating to 60 ℃, adding a stabilizer, stirring for 15min, adding the oil phase component of the emulsion prepared in the step (3), keeping the temperature at 60 ℃, stirring for 5min, adding a mineral mixture, a vitamin mixture and a pomegranate peel extract, continuously stirring and uniformly mixing for 8min at 60 ℃, passing through a colloid mill for 2 times, homogenizing for 2 times under high pressure of a high-pressure homogenizer at 35MPa, sterilizing and packaging to obtain the instant plant-based emulsion special for inflammatory bowel diseases.
Example 2
The raw materials of the instant plant-based emulsion special for inflammatory bowel diseases comprise: 40 parts of white chenopodium quinoa willd, 12.5 parts of compound vegetable oil (peanut oil and 12.5 parts of olive oil), 10 parts of soybean protein peptide, 20 parts of spirulina polysaccharide, 10 parts of pomegranate peel polyphenol extract, 5 parts of aureobasidium polysaccharide, 3 parts of emulsifier (lecithin and 2 parts of capric triglyceride), 3 parts of compound vitamin, 10 parts of compound mineral substance and 0.5 part of essence.
The preparation method of the instant plant-based emulsion special for inflammatory bowel diseases comprises the following steps:
(1) Soaking and cleaning quinoa wheat, spreading on soaked cotton cloth, germinating for 20 hours at 25 ℃, drying for 20 hours at 50 ℃, grinding to obtain germinated quinoa wheat powder, and adding the raw materials in a weight ratio of 1:2, steaming for 10 minutes, cooling to room temperature, and adding 10 7 Uniformly stirring cfu lactobacillus plantarum powder, fermenting for 36 hours at 37 ℃ to obtain germinated fermented quinoa wheat pulp, concentrating, drying, grinding, and carrying out superfine grinding treatment on the germinated fermented quinoa wheat powder by using a superfine grinder to prepare germinated fermented quinoa superfine powder with the particle size of 18-20 microns;
(2) Mixing the germinated and fermented quinoa micropowder with spirulina polysaccharide, aureobasidium pullulans polysaccharide and soybean protein peptide, adding water according to the material-liquid ratio of 1: 6, stirring for 10-15min, and colloid milling to obtain quinoa mixed slurry;
(3) Keeping the temperature of the compound vegetable oil at 60 ℃ for half an hour, adding a water-soluble emulsifier (the emulsifier can be dissolved just by adding water), continuously stirring for 15min to obtain an emulsion oil phase component, and keeping the temperature at 60 ℃ for later use;
(4) And (3) adding 3 times of water into the quinoa mixed slurry prepared in the step (2), heating to 60 ℃, adding a stabilizer, stirring for 18min, adding the oil phase component of the emulsion prepared in the step (3), keeping the temperature at 60 ℃, stirring for 5min, adding a mineral mixture, a vitamin mixture and a pomegranate peel extract, continuously stirring and uniformly mixing for 8min at 60 ℃, passing through a colloid mill for 2 times, homogenizing for 3 times under high pressure of 30MPa through a high-pressure homogenizer, sterilizing and packaging to obtain the instant plant-based emulsion special for the inflammatory bowel disease.
Example 3
The raw materials of the instant plant-based emulsion special for inflammatory bowel diseases comprise: 20 parts of rice, 5 parts of compound vegetable oil (soybean oil and 5 parts of walnut oil), 20 parts of soybean protein peptide, 15 parts of spirulina polysaccharide, 10 parts of pomegranate peel polyphenol extract, 5 parts of aureobasidium polysaccharide, 1 part of emulsifier (sucrose fatty acid ester and 1 part of capric triglyceride), 0.6 part of stabilizer (xanthan gum), 3 parts of vitamin complex, 10 parts of compound mineral substance and 0.5 part of essence.
The preparation method of the instant plant-based emulsion special for inflammatory bowel diseases comprises the following steps:
(1) Soaking and cleaning rice, spreading on soaked cotton cloth, germinating for 18 hours at 25 ℃, drying for 15 hours at 50 ℃, grinding to obtain germinated rice flour, and adding the germinated rice flour which is prepared from the following raw materials in a weight ratio of 1:3, steaming for 8 minutes, cooling to room temperature, and adding 10 6 Uniformly stirring cfu lactobacillus plantarum powder, fermenting for 30 hours at 37 ℃ to obtain germinated fermented rice slurry, concentrating, drying, grinding, and carrying out superfine grinding treatment on the germinated fermented rice by using a superfine grinder to prepare germinated fermented rice superfine powder with the particle size of 18-20 microns;
(2) Mixing the germinated and fermented rice superfine powder with spirulina polysaccharide, aureobasidium polysaccharide and soybean protein peptide, adding water according to the material-liquid ratio of 1;
(3) Keeping the temperature of the compound vegetable oil at 60 ℃ for half an hour, adding a water-soluble emulsifier (the emulsifier can be dissolved just by adding water), continuously stirring for 12min to obtain an emulsion oil phase component, and keeping the temperature at 60 ℃ for later use;
(4) Adding 4 times of volume of water into the rice mixed pulp prepared in the step (2), heating to 60 ℃, adding a stabilizer, stirring for 15min, adding the emulsion oil phase component prepared in the step (3), keeping the temperature at 60 ℃ and stirring for 3 min, adding a mineral mixture, a vitamin mixture and a pomegranate peel extract, continuously stirring and uniformly mixing for 5min at 60 ℃, passing through a colloid mill for 2 times, homogenizing for 2 times at 30MPa by a high-pressure homogenizer, sterilizing and packaging to obtain the instant plant-based emulsion special for the inflammatory bowel disease.
Comparative example 1:
the procedure of example 1 was repeated except that peeled foxtail millet was used instead of the foxtail millet in example 1, and the germination process was not performed.
Comparative example 2
The procedure of example 1 was otherwise the same as that of example 1, except that the millet in example 1 was not subjected to the germination treatment.
Comparative example 3
The same procedure as in example 1 was repeated except that the millet in example 1 was not subjected to saccharification and fermentation treatment.
Comparative example 4
The compound oil in example 1 was replaced with one of soybean oil, peanut oil and rapeseed oil, and the other operations were the same as in example 1.
Comparative example 5
The composite oil of example 1 was replaced with one of chia seed oil, linseed oil, olive oil and walnut oil, and the other operations were the same as example 1.
Animal experiments
60C 57BL/6 male mice of 7 weeks of age were randomly divided into 6 groups and adjusted twice according to body weight to ensure that the average body weight of each group was close. After one week of adaptive standard feed feeding, the emulsion products described in example 1 and comparative examples 1 to 5 were changed to the standard feed, and the standard feed was fed for one week while the inflammatory bowel disease induction was performed for five days by adding 3% dextran sulfate to drinking water, and the body weight change tendency and disease activity index were recorded during the induction period. Dissecting the mouse at the end of the induction period, fixing colon tissues, carrying out H & E staining after paraffin embedding, and observing the damage degree of the colonic epithelial structure by a microscope; five mice are randomly selected in each group, the whole cecum is taken, total DNA is extracted, a library is built after two rounds of PCR amplification, and the composition of the intestinal flora of the mice is identified by 16s rRNA sequencing.
1. Body weight changes during disease induction in mice
Body weight change during the onset of enteritis is an important indicator of disease severity. The weight loss rate calculation method is as follows:
FIG. 1 shows the rate of weight loss of mice during induction, with higher rates of weight loss indicating more severe disease progression in the mice. FIG. 1 shows that the body weight of the mice in the group of example 1 is almost maintained unchanged by the fifth day of induction, which indicates that the emulsion product of the present invention has a significant effect of preventing enteritis; the mice in the group of comparative example 1 had the highest weight loss rate, which indicates that the whole grain status is crucial to the prevention of enteritis in the mice; the weight loss rates of the mice of comparative examples 2 and 3 were higher than example 1, but lower than comparative example 1, indicating that the pretreatment of whole grain had a great effect on the function of the emulsion of the present invention, wherein the weight loss rate of the comparative example 2 group was higher than that of the comparative example 3 group, indicating that the fermentative saccharification treatment was more critical for reducing the rapid weight loss during the onset of enteritis than the germination treatment; comparative example 4 the group of comparative example 5 had a lower rate of weight loss compared to comparative example 5, indicating that the intake of soybean oil, peanut oil and rapeseed oil was more beneficial for weight maintenance.
2. Disease Activity index Change during disease Induction in mice
The disease activity index of the mouse during the induction period of the disease is obtained by respectively scoring and adding three indexes of the mouse fecal state, the mouse rectal bleeding state and the mouse weight loss state, and the scoring standards are as shown in the following table 1:
TABLE 1 mouse Disease Activity Index Scoring Standard Table (DAI)
According to the standard, the results of disease activity index in mice during induction of enteritis are shown in figure 1: the disease activity of the group of example 1 was lower than that of all the comparative groups, indicating that the emulsion of the present invention is effective in alleviating inflammatory bowel disease symptoms; the group of comparative example 1 exhibited the most severe symptoms, indicating that the effect of whole grain status on symptom relief was not negligible; the remaining four groups of comparative examples, namely comparative example 2> comparative example 5> comparative example 4> comparative example 3, evaluate the function of relieving enteritis symptoms, and illustrate that grain pretreatment and grease balance proportion bacteria in the examples have certain influence on the symptom relieving effect of the emulsion.
3. H & E staining results of mouse colonic epithelium
Pathological staining results of colon tissues of mice are shown in fig. 3 to 8, and the colon epithelial tissue structure of the mice fed with the diets in the group of the example 1 is complete, goblet cells are kept intact, and no obvious colon injury is seen; in the comparative example 1, complete colon epithelial tissue structure is difficult to observe under a microscope, goblet cells are completely disappeared, inflammatory cells are infiltrated in a large area, and the fact that compared with whole grains, peeled refined grains cannot play a role in preventing colitis; compared example 2 and comparative example 3, the damage of colon epithelium of the mouse is serious, most goblet cells disappear, and inflammatory cell infiltration is obvious, which shows that the process of germination or fermentation saccharification is crucial to the efficacy of the emulsion; comparative example 4 and comparative example 5, the colonic status of the mice was significantly better than comparative examples 2 and 3, with comparative example 4 being slightly worse, indicating that chia seed oil, linseed oil, olive oil and walnut oil are better than soybean oil, peanut oil and rapeseed oil for colonic injury.
4. Changes in intestinal flora in mice
The results of the evaluation of the composition of the mouse intestinal flora are shown in fig. 9 to 12, and as can be seen from fig. 9 to 12, compared with the comparative example, the composition of the mouse intestinal flora in example 1 can effectively inhibit the growth of Proteobacteria (Proteobacteria) and Shigella Escherichia coli (Shigella) which are pathogenic bacteria related to enteritis, and simultaneously promote the growth of lactic acid bacteria (Lactobacillus) and bifidobacteria (Bifidobacterium).
Claims (7)
1. The instant plant-based emulsion special for inflammatory bowel diseases is characterized by comprising the following raw materials in parts by weight: 20-50 parts of whole grain germinated fermentation superfine powder, 10-30 parts of compound vegetable oil and 0.5-5 parts of emulsifier;
the compound vegetable oil is a mixture of two groups A and B according to the mass ratio of 1;
the group A vegetable oil is more than one of soybean oil, peanut oil and rapeseed oil;
the B group vegetable oil is more than one of chia seed oil, linseed oil, olive oil and walnut oil;
the emulsifier is at least two of lecithin, sucrose fatty acid ester, monoglyceride and capric triglyceride; the stabilizer is at least one of xanthan gum, konjac gum and guar gum.
2. The instant plant-based emulsion special for inflammatory bowel diseases according to claim 1, wherein the raw materials further comprise 10-30 parts of soybean protein peptide, 10-30 parts of spirulina polysaccharide, 5-20 parts of pomegranate peel polyphenol extract, 1-10 parts of aureobasidium polysaccharide and 0.5-1 part of stabilizer.
3. The instant plant-based emulsion for inflammatory bowel disease according to claim 1 or 2, wherein the raw material further comprises one or more of complex minerals, complex vitamins and flavoring agents.
4. The instant plant-based emulsion for inflammatory bowel disease according to claim 1, wherein the whole grain is one or more of quinoa, millet and rice.
5. The special instant plant-based emulsion for inflammatory bowel diseases according to claim 1, wherein the particle size of the whole grain submicron powder is 18-20 μm.
6. A preparation method of the instant plant-based emulsion special for inflammatory bowel diseases, which is characterized by comprising the following steps:
(1) Soaking and cleaning the whole grain, spreading the whole grain on a soaked cotton cloth, germinating for 12 to 24 hours at 20 to 28 ℃, drying for 12 to 24 hours at 40 to 55 ℃, grinding the whole grain into powder to obtain germinated whole grain powder, and adding the germinated whole grain powder, wherein the weight ratio of the germinated whole grain powder to the grain raw material is 1: steaming for 5-15 minutes by using water of 1-3, cooling to room temperature, adding a leavening agent, uniformly stirring, fermenting at 37 ℃ for 24-48 hours to obtain germinated fermented grain slurry, concentrating, drying, grinding, and performing superfine grinding to prepare germinated fermented grain superfine powder with the grain size of 18-20 microns;
(2) Mixing the germinated and fermented grain superfine powder with spirulina polysaccharide, aureobasidium polysaccharide and soybean protein peptide, adding water according to the material-liquid ratio of 1;
(3) The compound vegetable oil is insulated for 20 to 40 minutes at the temperature of 55 to 65 ℃, then water is added into the oil and the emulsifier is dissolved, the mixture is continuously stirred for 10 to 20 minutes to obtain the oil phase component of the emulsion, and the temperature is kept at 55 to 65 ℃ for standby;
(4) Heating the grain mixed slurry prepared in the step (2) to 55-65 ℃, adding water and a stabilizer with the volume of 3-6 times, stirring for 15-20 minutes, adding the oil phase component of the emulsion prepared in the step (3), keeping the temperature at 55-65 ℃, stirring for 3-5 minutes, adding the compound mineral, the compound vitamin and the pomegranate peel extract, continuously stirring and uniformly mixing for 5-10 minutes at 55-65 ℃, grinding for 2-3 times by using a colloid mill, homogenizing for 2-3 times at high pressure under 25-35MPa by using a high-pressure homogenizer, uniformly sterilizing and packaging to obtain the instant plant-based emulsion special for the inflammatory bowel disease.
7. The method according to claim 6, wherein the fermentation inoculum in step (1) is Lactobacillus plantarum powder having an inoculation concentration of 10 per 1L of grain slurry 5 -10 7 cfu。
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