CN111012809A - Preparation method of ricepaperplant pith extract and pharmaceutical composition containing ricepaperplant pith extract - Google Patents
Preparation method of ricepaperplant pith extract and pharmaceutical composition containing ricepaperplant pith extract Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
- A23K20/147—Polymeric derivatives, e.g. peptides or proteins
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/612—Crustaceans, e.g. crabs, lobsters, shrimps, krill or crayfish; Barnacles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/19—Acanthaceae (Acanthus family)
- A61K36/195—Strobilanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
- A61K36/315—Isatis, e.g. Dyer's woad
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
- A61K36/533—Leonurus (motherwort)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/14—Drugs for genital or sexual disorders; Contraceptives for lactation disorders, e.g. galactorrhoea
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
Abstract
The invention discloses a method for preparing a medulla Tetrapanacis extract and a pharmaceutical composition containing the medulla Tetrapanacis extract, which comprises the following steps: grinding the ricepaperplant pith, performing low-temperature ball milling, and collecting ball-milled materials; adding the ball-milled material into ethanol for ball-milling extraction, and performing ultrasonic extraction on filter residues; obtaining a supernatant; carrying out high static pressure treatment on the supernatant; and (3) carrying out flash evaporation concentration and spray drying on the mixed supernatant after the high static pressure treatment to obtain the ricepaper pith extract. The method adopts a mode of combining ball milling extraction with ultrasonic extraction, has lower extraction temperature, and cannot damage the effective components of the ricepaperplant pith; the obtained medulla Tetrapanacis extract can be used in combination with other medicinal components to effectively improve lactation ability of sow and reduce breast diseases of sow.
Description
Technical Field
The invention relates to a preparation method of a medulla Tetrapanacis extract and a pharmaceutical composition containing the medulla Tetrapanacis extract, in particular to a pharmaceutical composition for sows prepared from the medulla Tetrapanacis extract.
Background
The feeding of the sows is one of the important links of pig raising production, and the healthy lactation of the sows is very important for the feeding of the sows. The key problem which puzzles the production of breeding pigs at present is that the breast diseases of the sows are frequent and the lactation capacity is low, so that the nutrition of piglets is lacked, the diarrhea rate is high, the growth is slow, the survival rate of the piglets is low, the weight of breast-breaking litter and the individual weight are small, the breast health and the lactation capacity of the sows are improved, the growth and development of suckling piglets and the improvement of the breeding efficiency of the sows are promoted, the cost can be further reduced, and the economic benefit is improved. However, due to the use of a large amount of antibiotics, the problems of drug resistance and drug residue become more serious, and at the same time, with the increasing call for pursuing high-quality agricultural products, green foods and the like by human beings, the development of a new nontoxic, harmless and residue-free feed additive becomes a new direction for promoting the healthy development of animal husbandry.
The ricepaperplant pith has sweet and light flavor, is slightly cool, tonifies without dryness, enters kidney and stomach channels, has the effects of clearing heat and promoting urination, and ventilating and promoting lactation, and is commonly used for treating damp-heat stranguria, painful urination, edema oliguria, agalactia and other symptoms in traditional Chinese medicine. Therefore, the method can be used for promoting the breast health and the lactation capacity of sows by preparing the ricepaperplant pith extract and assisting other traditional Chinese medicine compositions, solves the problem of antibiotic residue which troubles the development of the pig industry for a long time, and reduces the pollution to the environment.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for preparing a medulla Tetrapanacis extract, comprising the steps of:
crushing the medulla Tetrapanacis, mixing the crushed medulla Tetrapanacis particles with sodium hydroxide, adding the obtained mixture into a ball milling tank, and ball-milling balls in the ball milling tank; introducing liquid nitrogen into the ball milling tank to immerse the ricepaperplant pith particles and the sodium hydroxide in the liquid nitrogen at the temperature of-160 to-180 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; keeping the temperature for 20-25 min, and then starting ball milling for 45-60 min; after ball milling is finished, transferring the ball milling tank into a vacuum glove box, standing for 12-24 hours, and collecting ball milling materials;
adding the ball-milled material into the ethanol A, ultrasonically soaking for 10-15 min, then simultaneously adding the ball-milled material and the ethanol A into a ball-milling tank, adding ball-milling balls into the ball-milling tank, ball-milling for 30-60 min, and centrifuging the ball-milled material to obtain supernatant A and filter residue A; adding the filter residue A and the ethanol B into a ball milling tank at the same time, adding ball milling balls into the ball milling tank, and carrying out ball milling for 20-30 min; centrifuging the ball-milled materials to obtain a supernatant B and a filter residue B; adding the filter residue B into an ultrasonic extractor, adding magnetized water at the same time, performing ultrasonic extraction for 10-15 min, and centrifuging the extracted material to obtain a supernatant C;
mixing the supernatant A, the supernatant B and the supernatant C to obtain mixed supernatant, adding the mixed supernatant into a vacuum packaging bag for vacuum packaging, and controlling the vacuum degree to be 0.1 MPa; putting the vacuum packaging bag into high static pressure treatment equipment, sealing a pressurizing cavity, and pressurizing to perform high static pressure treatment;
and step four, carrying out flash evaporation concentration on the mixed supernatant subjected to high static pressure treatment by using a vacuum film concentration device under the conditions of 50-80 ℃ and 0.08-0.09 Mpa of vacuum degree, adjusting the liquid inlet speed of the mixed supernatant, repeatedly concentrating the mixed supernatant to 1/6-1/10 of the original volume at the flow rate of 120-200 mL/min, and carrying out spray drying on the mixed supernatant to obtain the ricepaperplant pith extract.
Preferably, in the first step, the weight ratio of the ricepaperplant pith particles to the sodium hydroxide is 1: 0.01-0.03; the dosage of the ball grinding balls is 20-50 ball grinding balls added in each 1g of ricepaper pith granules; the ball milling tank and the ball milling balls are made of agate or zirconia; the rotating speed of the ball milling is 300-500 r/min.
Preferably, in the second step, the ethanol A is 75-85% ethanol solution; the ethanol B is an 85-95% ethanol solution; the magnetized water is 12000-15000 GS magnetized water.
Preferably, in the second step, the mass-to-volume ratio of the ball-milling material to the ethanol A is 1g: 30-60 mL; the mass-volume ratio of the filter residue A to the ethanol B is 1g: 50-80 mL; the amount of the ball milling balls is 20-50 ball milling balls added into each 1g of ball milling material or each 1g of filter residue A; the ball milling tank and the ball milling balls are made of agate or zirconia; the rotation speed of the ball milling is 300-500 r/min.
Preferably, in the second step, the ultrasonic power of the ultrasonic soaking is 300-400W, and the ultrasonic frequency is 30-35 kHz; the extraction power of ultrasonic extraction is 500-600W, the extraction frequency is 45-60 kHz, and the extraction temperature is 50-60 ℃; the dosage of the magnetized water is 50-80 mL of magnetized water added into each 1g of filter residue B.
Preferably, in the third step, the parameters of the high static pressure treatment are as follows: raising the pressure to 450-600 MPa at a pressure raising speed of 2-4 MPa/s, and carrying out pressure maintaining treatment for 15-30 min at normal temperature.
The invention also provides a pharmaceutical composition containing the medulla Tetrapanacis extract, which comprises the following raw materials in parts by weight: 30-50 parts of a medulla Tetrapanacis extract, 15-25 parts of radix isatidis, 10-20 parts of motherwort herb, 5-10 parts of melastoma dodecandrum lour, 1-5 parts of antimicrobial peptide and 5-10 parts of shrimp shell powder.
The invention also provides a preparation method of the pharmaceutical composition containing the ricepaperplant pith extract, which comprises the following steps: according to the weight parts, 30-50 parts of a medulla Tetrapanacis extract, 15-25 parts of radix isatidis, 10-20 parts of motherwort herb, 5-10 parts of melastoma dodecandrum lour, 1-5 parts of antimicrobial peptide and 5-10 parts of shrimp shell powder are stirred and mixed uniformly to obtain the pharmaceutical composition.
The invention provides a using method of the pig pharmaceutical composition containing the ricepaperplant pith extract, which comprises the following steps: adding the pharmaceutical composition into animal feed, and uniformly stirring, wherein the dosage of the pharmaceutical composition is 3-5 g per 100g of the animal feed.
The invention at least comprises the following beneficial effects: the method adopts a mode of combining ball milling extraction with ultrasonic extraction, has lower extraction temperature, and cannot damage the effective components of the ricepaperplant pith; the obtained medulla Tetrapanacis extract can be used in combination with other medicinal components to effectively improve lactation ability of sow and reduce breast diseases of sow.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
The specific implementation mode is as follows:
the present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1:
a preparation method of a medulla Tetrapanacis extract comprises the following steps:
crushing medulla Tetrapanacis, mixing 100g of crushed medulla Tetrapanacis particles with 2g of sodium hydroxide, adding the mixture into a ball milling tank, and ball-milling balls in the ball milling tank; introducing liquid nitrogen into the ball milling tank to immerse the ricepaperplant pith particles and the sodium hydroxide in the liquid nitrogen at-170 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; ball milling is started after the constant temperature is kept for 25min, and the ball milling is carried out for 60 min; after ball milling is finished, transferring the ball milling tank into a vacuum glove box, standing for 24 hours, and collecting ball milling materials; the dosage of the ball grinding balls is 30 ball grinding balls added in each 1g of ricepaper pith granules; the ball milling tank and the ball milling balls are made of agate; the rotating speed of the ball milling is 500 r/min; the mode of liquid nitrogen low-temperature ball milling is adopted, the powder adhesion phenomenon can be eliminated, the grinding efficiency is improved, the low-temperature ball milling has a refining effect on the size of powder particles, and the uniform mixing of the ricepaperplant pith particles and the sodium hydroxide can be further improved.
Step two, adding 50g of ball-milled material into 2500mL of ethanol A, ultrasonically soaking for 15min, then simultaneously adding the ball-milled material and the ethanol A into a ball-milling tank, adding ball-milling balls into the ball-milling tank, carrying out ball milling for 60min, and centrifuging the ball-milled material to obtain supernatant A and filter residue A; simultaneously adding 30g of filter residue A and 1500mL of ethanol B into a ball milling tank, adding ball milling balls into the ball milling tank, and carrying out ball milling for 30 min; centrifuging the ball-milled materials to obtain a supernatant B and a filter residue B; adding 20g of filter residue B into an ultrasonic extractor, simultaneously adding 2000mL of magnetized water, performing ultrasonic extraction for 15min, and centrifuging the extracted material to obtain supernatant C; the ethanol A is 80% ethanol solution; the ethanol B is 90% ethanol solution; the magnetized water is 15000GS magnetized water; the dosage of the ball milling balls is 30 ball milling balls added into each 1g of ball milling material or each 1g of filter residue A; the ball milling tank and the ball milling balls are made of agate; the rotating speed of ball milling is 500 r/min; the ultrasonic power of the ultrasonic soaking is 300W, and the ultrasonic frequency is 35 kHz; the extraction power of the ultrasonic extraction is 600W, the extraction frequency is 60kHz, and the extraction temperature is 50 ℃; the filter residue B is extracted by using the magnetized water, the pH value of the magnetized water is high, the association degree of the magnetized water is reduced, larger water molecule groups are converted into smaller water molecule groups, even single water molecules, the solubility is increased, the osmotic pressure is improved, and the effective extraction of the component of the filter residue B can be improved;
mixing the supernatant A, the supernatant B and the supernatant C to obtain mixed supernatant, adding the mixed supernatant into a vacuum packaging bag for vacuum packaging, and controlling the vacuum degree to be 0.1 MPa; putting the vacuum packaging bag into high static pressure treatment equipment, sealing a pressurizing cavity, and pressurizing to perform high static pressure treatment; the parameters of the high hydrostatic pressure treatment were: raising the pressure to 600MPa at a pressure raising speed of 2MPa/s, and maintaining the pressure for 15min at normal temperature;
and step four, carrying out flash evaporation concentration on the mixed supernatant subjected to high static pressure treatment by using a vacuum film concentration device under the conditions of 60 ℃ and 0.08Mpa of vacuum degree, adjusting the liquid inlet speed of the mixed supernatant, repeatedly concentrating the mixed supernatant to 1/6 of the original volume at the flow rate of 200mL/min, and carrying out spray drying on the mixed supernatant to obtain the ricepaperplant pith extract.
Example 2:
a preparation method of a medulla Tetrapanacis extract comprises the following steps:
crushing medulla Tetrapanacis, mixing 100g of crushed medulla Tetrapanacis particles with 1g of sodium hydroxide, adding the obtained mixture into a ball milling tank, and ball-milling balls in the ball milling tank; introducing liquid nitrogen into the ball milling tank to immerse the ricepaperplant pith particles and the sodium hydroxide in the liquid nitrogen at-165 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; ball milling is started after the constant temperature is kept for 20min, and the ball milling is carried out for 45 min; after ball milling is finished, transferring the ball milling tank into a vacuum glove box, standing for 12 hours, and collecting ball milling materials; the dosage of the ball grinding balls is 40 ball grinding balls added in each 1g of ricepaper pith granules; the ball milling tank and the ball milling balls are made of agate; the rotating speed of the ball mill is 400 r/min;
step two, adding 60g of ball-milled material into 1800mL of ethanol A, ultrasonically soaking for 10min, then simultaneously adding the ball-milled material and the ethanol A into a ball-milling tank, adding ball-milling balls into the ball-milling tank, carrying out ball milling for 45min, and centrifuging the ball-milled material to obtain supernatant A and filter residue A; simultaneously adding 40g of filter residue A and 2000mL of ethanol B into a ball milling tank, adding ball milling balls into the ball milling tank, and carrying out ball milling for 30 min; centrifuging the ball-milled materials to obtain a supernatant B and a filter residue B; adding 30g of filter residue B into an ultrasonic extractor, simultaneously adding 1500mL of magnetized water, performing ultrasonic extraction for 15min, and centrifuging the extracted material to obtain supernatant C; ethanol A is 85% ethanol solution; the ethanol B is a 95% ethanol solution; the magnetized water is 15000GS magnetized water; the dosage of the ball milling balls is 30 ball milling balls added into each 1g of ball milling material or each 1g of filter residue A; the ball milling tank and the ball milling balls are made of agate; the rotating speed of ball milling is 500 r/min; the ultrasonic power of the ultrasonic soaking is 400W, and the ultrasonic frequency is 35 kHz; the extraction power of the ultrasonic extraction is 500W, the extraction frequency is 45kHz, and the extraction temperature is 50 ℃;
mixing the supernatant A, the supernatant B and the supernatant C to obtain mixed supernatant, adding the mixed supernatant into a vacuum packaging bag for vacuum packaging, and controlling the vacuum degree to be 0.1 MPa; putting the vacuum packaging bag into high static pressure treatment equipment, sealing a pressurizing cavity, and pressurizing to perform high static pressure treatment; the parameters of the high hydrostatic pressure treatment were: raising the pressure to 500MPa at a pressure raising speed of 4MPa/s, and maintaining the pressure for 15min at normal temperature;
and step four, carrying out flash evaporation concentration on the mixed supernatant subjected to high static pressure treatment by using a vacuum film concentration device under the conditions of 60 ℃ and 0.08Mpa of vacuum degree, adjusting the liquid inlet speed of the mixed supernatant, repeatedly concentrating the mixed supernatant to 1/7 of the original volume at the flow rate of 200mL/min, and carrying out spray drying on the mixed supernatant to obtain the ricepaperplant pith extract.
Example 3:
a preparation method of a medulla Tetrapanacis extract comprises the following steps:
crushing medulla Tetrapanacis, mixing 100g of crushed medulla Tetrapanacis particles with 3g of sodium hydroxide, adding the obtained mixture into a ball milling tank, and ball-milling balls in the ball milling tank; introducing liquid nitrogen into the ball milling tank to immerse the ricepaperplant pith particles and the sodium hydroxide in the liquid nitrogen at-180 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; ball milling is started after the constant temperature is kept for 20min, and the ball milling is carried out for 40 min; after ball milling is finished, transferring the ball milling tank into a vacuum glove box, standing for 12 hours, and collecting ball milling materials; the dosage of the ball grinding balls is 50 ball grinding balls added in each 1g of ricepaper pith granules; the ball milling tank and the ball milling balls are made of agate; the rotation speed of the ball milling is 450 r/min;
step two, adding 50g of ball-milled material into 3000mL of ethanol A, ultrasonically soaking for 10min, then simultaneously adding the ball-milled material and the ethanol A into a ball-milling tank, adding ball-milling balls into the ball-milling tank, carrying out ball milling for 45min, and centrifuging the ball-milled material to obtain supernatant A and filter residue A; simultaneously adding 40g of filter residue A and 3200mL of ethanol B into a ball milling tank, adding ball milling balls into the ball milling tank, and carrying out ball milling for 30 min; centrifuging the ball-milled materials to obtain a supernatant B and a filter residue B; adding 20g of filter residue B into an ultrasonic extractor, simultaneously adding 1000mL of magnetized water, performing ultrasonic extraction for 15min, and centrifuging the extracted material to obtain supernatant C; the ethanol A is 80% ethanol solution; the ethanol B is 90% ethanol solution; the magnetized water is 15000GS magnetized water; the dosage of the ball milling balls is 30 ball milling balls added into each 1g of ball milling material or each 1g of filter residue A; the ball milling tank and the ball milling balls are made of agate; the rotating speed of ball milling is 500 r/min; the ultrasonic power of the ultrasonic soaking is 400W, and the ultrasonic frequency is 35 kHz; the extraction power of the ultrasonic extraction is 500W, the extraction frequency is 45kHz, and the extraction temperature is 50 ℃;
mixing the supernatant A, the supernatant B and the supernatant C to obtain mixed supernatant, adding the mixed supernatant into a vacuum packaging bag for vacuum packaging, and controlling the vacuum degree to be 0.1 MPa; putting the vacuum packaging bag into high static pressure treatment equipment, sealing a pressurizing cavity, and pressurizing to perform high static pressure treatment; the parameters of the high hydrostatic pressure treatment were: raising the pressure to 500MPa at a pressure raising speed of 3MPa/s, and maintaining the pressure for 15min at normal temperature;
and step four, carrying out flash evaporation concentration on the mixed supernatant subjected to high static pressure treatment by using a vacuum film concentration device under the conditions of 60 ℃ and 0.08Mpa of vacuum degree, adjusting the liquid inlet speed of the mixed supernatant, repeatedly concentrating the mixed supernatant to 1/8 of the original volume at the flow rate of 200mL/min, and carrying out spray drying on the mixed supernatant to obtain the ricepaperplant pith extract.
Example 4:
preparing a pharmaceutical composition using the extract of ricepaperplant pith prepared in example 1; the method comprises the following steps: according to the weight, 30g of a medulla Tetrapanacis extract, 15g of isatis root, 20g of motherwort, 10g of melastoma dodecandrum, 2g of antimicrobial peptide and 8g of shrimp shell powder are stirred and mixed uniformly to obtain the pharmaceutical composition.
Example 5:
preparing a pharmaceutical composition using the extract of ricepaperplant pith prepared in example 2; the method comprises the following steps: according to the weight, 50g of a medulla Tetrapanacis extract, 25g of radix isatidis, 15g of motherwort herb, 8g of melastoma dodecandrum lour, 3g of antimicrobial peptide and 10g of shrimp shell powder are stirred and mixed uniformly to obtain the pharmaceutical composition.
Example 6:
preparing a pharmaceutical composition using the extract of ricepaperplant pith prepared in example 3; the method comprises the following steps: according to the weight, 40g of a medulla Tetrapanacis extract, 20g of radix isatidis, 18g of motherwort herb, 6g of melastoma dodecandrum lour, 1g of antimicrobial peptide and 8g of shrimp shell powder are stirred and mixed uniformly to obtain the pharmaceutical composition.
Example 7:
the pharmaceutical composition prepared in example 4 is mixed with pig feed and fed to sows in the lactation period, and the dosage of the pharmaceutical composition is 3g of pharmaceutical composition added into 100g of animal feed;
the pig feed comprises the following components in parts by weight: 50kg of corn flour, 20kg of wheat bran, 10kg of fish meal, 2kg of calcium hydrophosphate, 10kg of peanut cake and 10kg of soybean.
Example 8:
the pharmaceutical composition prepared in example 5 is mixed with pig feed and fed to sows in the lactation period, and the dosage of the pharmaceutical composition is 3g of pharmaceutical composition added into 100g of animal feed;
the pig feed comprises the following components in parts by weight: 50kg of corn flour, 20kg of wheat bran, 10kg of fish meal, 2kg of calcium hydrophosphate, 10kg of peanut cake and 10kg of soybean.
Example 9:
the pharmaceutical composition prepared in example 6 is mixed with pig feed and fed to sows in the lactation period, and the dosage of the pharmaceutical composition is 3g of pharmaceutical composition added into 100g of animal feed;
the pig feed comprises the following components in parts by weight: 50kg of corn flour, 20kg of wheat bran, 10kg of fish meal, 2kg of calcium hydrophosphate, 10kg of peanut cake and 10kg of soybean.
Comparative example 1:
the pig feed comprises the following components by weight: 50kg of corn flour, 20kg of wheat bran, 10kg of fish meal, 2kg of calcium hydrophosphate, 10kg of peanut cake and 10kg of soybean.
Comparative example 2:
the pig feed is fed after the pharmaceutical composition and the pig feed are mixed according to the following weight parts;
the pharmaceutical composition comprises: stirring and uniformly mixing 15g of isatis root, 20g of motherwort herb, 10g of melastoma dodecandrum lour, 2g of antimicrobial peptide and 8g of shrimp shell powder to obtain a medicinal composition;
pig feed: 50kg of corn flour, 20kg of wheat bran, 10kg of fish meal, 2kg of calcium hydrophosphate, 10kg of peanut cake and 10kg of soybean.
The dosage of the pharmaceutical composition is 3g of the pharmaceutical composition added into every 100g of the pig feed.
Feeding lactating sows with mixed feed of the pharmaceutical composition of examples 7-9 and pig feed and the pig feed of comparative example 1; the experiment was carried out in a pig farm in Pingwu county, Mianyang, and 200 sows to be produced were selected and evenly divided into 4 groups of 50 pigs each; feeding the mixed feed of the examples 7-9 and the feed of the comparative example 1 starting 1 week before production until the piglets are weaned for 21 days; counting the weight and physical condition of the piglets, wherein the results are shown in table 1;
TABLE 1
As can be seen from Table 1, the mixed feeding of the pharmaceutical composition of the invention and the pig feed can significantly reduce the number of breast diseases of the sow during lactation, significantly improve the survival rate and the weight of piglets, and indirectly reflect the improvement of the lactation capability of the sow.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the examples shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (9)
1. A preparation method of a ricepaperplant pith extract is characterized by comprising the following steps:
crushing the medulla Tetrapanacis, mixing the crushed medulla Tetrapanacis particles with sodium hydroxide, adding the obtained mixture into a ball milling tank, and ball-milling balls in the ball milling tank; introducing liquid nitrogen into the ball milling tank to immerse the ricepaperplant pith particles and the sodium hydroxide in the liquid nitrogen at the temperature of-160 to-180 ℃, and keeping the volatilization amount and the introduction amount of the liquid nitrogen balanced to stabilize the liquid level; keeping the temperature for 20-25 min, and then starting ball milling for 45-60 min; after ball milling is finished, transferring the ball milling tank into a vacuum glove box, standing for 12-24 hours, and collecting ball milling materials;
adding the ball-milled material into the ethanol A, ultrasonically soaking for 10-15 min, then simultaneously adding the ball-milled material and the ethanol A into a ball-milling tank, adding ball-milling balls into the ball-milling tank, ball-milling for 30-60 min, and centrifuging the ball-milled material to obtain supernatant A and filter residue A; adding the filter residue A and the ethanol B into a ball milling tank at the same time, adding ball milling balls into the ball milling tank, and carrying out ball milling for 20-30 min; centrifuging the ball-milled materials to obtain a supernatant B and a filter residue B; adding the filter residue B into an ultrasonic extractor, adding magnetized water at the same time, performing ultrasonic extraction for 10-15 min, and centrifuging the extracted material to obtain a supernatant C;
mixing the supernatant A, the supernatant B and the supernatant C to obtain mixed supernatant, adding the mixed supernatant into a vacuum packaging bag for vacuum packaging, and controlling the vacuum degree to be 0.1 MPa; putting the vacuum packaging bag into high static pressure treatment equipment, sealing a pressurizing cavity, and pressurizing to perform high static pressure treatment;
and step four, carrying out flash evaporation concentration on the mixed supernatant subjected to high static pressure treatment by using a vacuum film concentration device under the conditions of 50-80 ℃ and 0.08-0.09 Mpa of vacuum degree, adjusting the liquid inlet speed of the mixed supernatant, repeatedly concentrating the mixed supernatant to 1/6-1/10 of the original volume at the flow rate of 120-200 mL/min, and carrying out spray drying on the mixed supernatant to obtain the ricepaperplant pith extract.
2. The method for preparing the medulla Tetrapanacis extract as claimed in claim 1, wherein in the first step, the weight ratio of the medulla Tetrapanacis particles to the sodium hydroxide is 1: 0.01-0.03; the dosage of the ball grinding balls is 20-50 ball grinding balls added in each 1g of ricepaper pith granules; the ball milling tank and the ball milling balls are made of agate or zirconia; the rotating speed of the ball milling is 300-500 r/min.
3. The method for preparing a medulla Tetrapanacis extract as claimed in claim 1, wherein in the second step, the ethanol A is 75-85% ethanol solution; the ethanol B is an 85-95% ethanol solution; the magnetized water is 12000-15000 GS magnetized water.
4. The preparation method of the medulla Tetrapanacis extract as claimed in claim 1, wherein in the second step, the mass-to-volume ratio of the ball-milled material to the ethanol A is 1g: 30-60 mL; the mass-volume ratio of the filter residue A to the ethanol B is 1g: 50-80 mL; the amount of the ball milling balls is 20-50 ball milling balls added into each 1g of ball milling material or each 1g of filter residue A; the ball milling tank and the ball milling balls are made of agate or zirconia; the rotation speed of the ball milling is 300-500 r/min.
5. The preparation method of the medulla Tetrapanacis extract as claimed in claim 1, wherein in the second step, the ultrasonic power of the ultrasonic soaking is 300-400W, and the ultrasonic frequency is 30-35 kHz; the extraction power of ultrasonic extraction is 500-600W, the extraction frequency is 45-60 kHz, and the extraction temperature is 50-60 ℃; the dosage of the magnetized water is 50-80 mL of magnetized water added into each 1g of filter residue B.
6. The method for preparing the medulla Tetrapanacis extract as claimed in claim 1, wherein the parameters of the high static pressure treatment in the third step are as follows: raising the pressure to 450-600 MPa at a pressure raising speed of 2-4 MPa/s, and carrying out pressure maintaining treatment for 15-30 min at normal temperature.
7. A pharmaceutical composition containing the ricepaperplant pith extract as claimed in any one of claims 1-6, which is characterized by comprising the following raw materials in parts by weight: 30-50 parts of a medulla Tetrapanacis extract, 15-25 parts of radix isatidis, 10-20 parts of motherwort herb, 5-10 parts of melastoma dodecandrum lour, 1-5 parts of antimicrobial peptide and 5-10 parts of shrimp shell powder.
8. A method for preparing the pharmaceutical composition containing the extract of ricepaperplant pith as claimed in claim 7, which comprises: according to the weight parts, 30-50 parts of a medulla Tetrapanacis extract, 15-25 parts of radix isatidis, 10-20 parts of motherwort herb, 5-10 parts of melastoma dodecandrum lour, 1-5 parts of antimicrobial peptide and 5-10 parts of shrimp shell powder are stirred and mixed uniformly to obtain the pharmaceutical composition.
9. A method of using the medulla Tetrapanacis extract-containing pharmaceutical composition for swine of claim 8, comprising: adding the pharmaceutical composition into animal feed, and uniformly stirring, wherein the dosage of the pharmaceutical composition is 3-5 g per 100g of the animal feed.
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