CN111011311A - 亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用 - Google Patents
亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用 Download PDFInfo
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Abstract
本发明属于生物科技领域,具体公开了亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用。申请人通过利用水虻转化含有藻毒素的蓝藻藻泥,发现水虻对蓝藻藻泥中藻毒素有一定降解作用,降解率为40.98%,水虻转化蓝藻藻泥后,只有0.81%的藻毒素转移到水虻体内,转移量小。通过麸皮、玉米皮与蓝藻藻泥进行一定比例的配比来饲喂亮斑扁角水虻幼虫,对蓝藻藻泥进行转化,能取得较佳的转化效果,本发明的转化方法,不仅能够有效转化蓝藻藻泥,获得水虻虫体蛋白,转化周期仅需12‑13天,而且还可以降解蓝藻藻泥中的藻毒素,解决蓝藻藻泥对环境的污染以及对动物和人类健康的影响。
Description
技术领域
本发明属于生物科技领域,具体涉及亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用,本发明不仅能解决湖泊中蓝藻藻泥泛滥的污染问题,而且成本低廉,并且能够得到高附加值昆虫蛋白,减少蓝藻藻泥和藻毒素对环境的污染。
背景技术
夏秋季,是富营养化水体蓝藻水华高发期,一些湖库不得不收获水华蓝藻形成大量藻泥,其中一些藻泥含有藻毒素,其含量可达干重的千分之一。藻毒素具有促癌作用、肝毒性、肾毒性、肠毒性以及其它毒性。现有的蓝藻藻泥处理技术,如厌氧发酵等,极易造成二次污染。藻毒素的去除是困扰蓝藻藻泥处置的难点之一,目前还没有可靠环保的去除蓝藻藻泥中藻毒素的处理技术被报道,所以急需找到一种高效、低成本、无害化的处理办法。
有文献报道,利用裂殖壶藻藻渣饲养黑水虻能提升黑水虻油脂营养价值。当黑水虻幼虫日粮中裂殖壶藻藻渣的添加水平不超过10%时,不会对幼虫的生长造成负面影响,还能显著提高幼虫体内n-3多不饱和脂肪酸的含量。藻渣添加量达20%、30%、40%时,其幼虫粗蛋白质及粗脂肪含量显著低于不添加藻渣的对照组,但文献中并没有分析其原因,只得出了黑水虻幼虫日粮中藻渣的适宜添加水平在10%以内为宜的结论。
由于蓝藻藻泥含水率高达95%,且其干物质中蛋白质含量高达50%以上,较粘稠,还含有藻毒素是极为难去除的原料,用水虻处理纯蓝藻藻泥转化效率低。主要原因是蓝藻藻泥营养不平衡,粘稠不透气,含有藻毒素等因素。
从已知的文献中还没有发现水虻降解藻毒素的报道,本发明中是首次发现水虻可降解藻毒素。
发明内容
本发明提供了亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用,本发明提供的方法不仅能解决蓝藻藻泥泛滥的污染问题,而且成本低廉,能够得到高附加值值得昆虫蛋白,而且还可以降解蓝藻藻泥中的藻毒素,解决蓝藻藻泥对环境的污染以及对动物和人类健康的影响。
为了达到上述目的,本发明采取以下技术措施:
亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用,包括将亮斑扁角水虻添加至含有蓝藻藻泥的基质中进行转化,在水虻转化的同时还可降解藻泥中的藻毒素。
以上所述的应用中,优选的,所述的基质含有除蓝藻藻泥的其他辅料;所述的辅料为:
1)麸皮;或2)玉米皮和菌渣;
以上所述的应用中,优选的,蓝藻藻泥:麸皮干重比=1:5-10;或蓝藻藻泥:玉米皮:菌渣干重比=1:7-20:1-3;
以上所述的应用中,优选的,所述的基质的含水率为65-85%;
以上所述的应用中,优选的,所述的亮斑扁角水虻为亮斑扁角水虻武汉品系的8日龄水虻幼虫。
与现有技术相比,本发明具有以下优点:
本发明中,搭配氮含量较高且蓬松的麸皮、玉米皮和碳含量较高金针菇菌渣与蓝藻藻泥进行配方,调整其含水率,达到合适的碳氮比,提供通气性,以期达到水虻幼虫的高存活率和物料的高转化率。
亮斑扁角水虻的武汉品系,具有食谱广、食量大、转化率高、转化周期短、无害化水平高的特点。利用武汉亮斑扁角水虻处理蓝藻藻泥,不仅可以实现蓝藻藻泥的无害化处理,还能将蓝藻藻泥转化为高水平的动物蛋白,一举两得。但是仅仅利用蓝藻藻泥来饲养水虻,存在转化周期长、转化率偏低的问题。本发明采用蓝藻藻泥与麸皮、玉米皮等下脚料配比,找到一个合适的配方来饲喂水虻,来达到较高的转化率,并缩短转化周期,并且得到高质量的水虻虫体蛋白和优质有机肥料,同时考察水虻转化蓝藻藻泥对蓝藻藻泥中藻毒素是否有降解作用。
本发明利用亮斑扁角水虻幼虫转化蓝藻藻泥。通过麸皮、玉米皮与蓝藻藻泥进行一定比例的配比来饲喂亮斑扁角水虻幼虫,对蓝藻藻泥泥进行转化。物料减少率高达50%以上,水虻转化率高达6%-11.5%,转化周期短,仅需12-13天。
亮斑扁角水虻幼虫转化蓝藻藻泥的同时,还可以降解蓝藻藻泥中的藻毒素。
亮斑扁角水虻幼虫转化蓝藻藻泥的同时,还得到了高附加值值的昆虫蛋白,具有实际的生产价值。
转化后的物料和虫粪可用于生产有机肥,实现零排放,不会造成二次污染。
具体实施方式
以下结合具体的实施例和实验数据对本发明做进一步的说明。本发明实施例中所述技术方案,如未特别说明,均为常规技术方案,所用试剂或配方若未特别说明,均购自生化商店。
本发明实施例中:
所述8日龄幼虫为亮斑扁角水虻武汉品系,(Fen Zhou,Jeffery K.Tomberlin,Longyu Zheng,Ziniu Yu,and Jibin Zhang,Developmental and Waste ReductionPlasticity of Three Black Soldier Fly Strains(Diptera:Stratiomyidae)Raised onDifferent Livestock Manures.Journal of Medical Entomology,2013,50(6):1224-1230.),约28mg/头;
本发明实施例所用蓝藻藻泥来自中国科学院水生生物研究所,是从太湖梅梁湾收获的水华蓝藻(Microcystis)。该水华蓝藻经浓缩脱水制成藻粉。有毒藻粉优势种为铜绿微囊藻(Microcystisaeuginosa),无毒藻粉优势种为水华微囊藻(Microcystisflosaquae)。
藻泥含水率96.3%和95.0%(两批不同藻泥,具体在实施例中标出);蓝藻藻粉含水率10%;麸皮购自市场,含水率10%。玉米皮购自市场,含水率11%。金针菇菌渣购自市场,含水率为52%。
实验在水虻转化容器(直径10.5cm×高13cm)内进行,控制室温28±2℃,环境湿度40%-60%,遮光。
实施例1:
亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用
①用有毒藻粉(优势种为铜绿微囊藻Microcystisaeuginosa)按干重10%比例与麸皮进行配比,再加水,将含水率调至70%,具体配方见下表:
表1水虻转化蓝藻藻泥的配方
②每个桶加入混合饲料后,再加入400头水虻幼虫(8日龄),任其自由取食,每组4个重复。控制室温28±2℃,环境湿度40%-60%。
③取样。取原始物料、原始幼虫,再取第10天的转化后物料和水虻幼虫。
④分别统计每组物料减少率、水虻转化率、幼虫死亡率。
⑤测量每组样品物料和幼虫的藻毒素含量。
⑥结果的统计与分析。
表2转化前后物料和虫体中藻毒素总含量
藻毒素降解率公式如下:
藻毒素降解率=(转化前物料藻毒素总含量-转化后第10天物料藻毒素总含量-转化后第10天虫体藻毒素总含量)/转化前原始物料藻毒素总含量×100%
经测量,蓝藻藻泥经过亮斑扁角水虻10天的转化,藻毒素降解率为40.98%。仅有0.81%的藻毒素转移到水虻体内,转移量小。
表3水虻转化效率评价
依据以下公式计算相关转化指标:
水虻转化率=(转化后水虻干重-转化前水虻干重)/原始物料干重x 100%
物料减少率=(转化后物料干重-转化前物料干重)/转化前物料干重x 100%
幼虫死亡率=(转化前水虻数量-转化后水虻数量)/转化前水虻数量x 100%
经测量,经亮斑扁角水虻10天的转化,物料减少率为46.12%,水虻转化率为13.44%,幼虫死亡率仅2.50%,较符合实际生产的需要。
实施例2:
亮斑扁角水虻幼虫在转化蓝藻藻泥的应用:
步骤如下:
①蓝藻藻泥(无毒蓝藻,主要为水华微囊藻,Microcystisflos-aquae)含水率96.3%,定量麸皮量为100g,加入不同量的蓝藻藻泥,同时调节含水率在65-80%之间,其比例设置如下:
表3水虻幼虫转化蓝藻藻泥和麸皮复配的配方组成
注:表中麸皮量、蓝藻藻泥量均为鲜重
②每个桶加入混合饲料后,再加入200头水虻幼虫(8日龄),任其自由取食,每组4个重复。控制室温28±2℃,环境湿度40%-60%。
③待出现50%的预蛹后,虫料分离。
④分别统计每组的50%预蛹时间、物料减少率、水虻转化率、幼虫死亡率。
⑤结果的统计与分析。
表4蓝藻藻泥和麸皮不同配比下水虻转化效率评价
注:表中数据为上次重复平均值±标准误,数据之间进行单因素方差分析,同行数据的不同小写字母表示在0.05水平上差异性显著。
①随着蓝藻藻泥量的增加,水虻转化率呈下降趋势,说明蓝藻藻泥及其其中的藻毒素对水虻的转化效率具一定的负面影响;但是从物料减少情况来看,蓝藻藻泥的增加对物料减少的影响不大,其中,C组的物料减少率显著高于其他各组,即蓝藻藻泥:麸皮干重比=1:7.5时,其物料消耗最好。
②显然,D和E组的水虻幼虫死亡率很高,分别为21.83%和38.00%,说明,当藻液重量:麸皮重量为4:1和5:1时,水虻出现了大量死亡现象,这可能与蓝藻藻泥中的藻毒素量有关;并且D和E组的转化时间分别为23.8d和44.6d,转化时间大大延长,影响处理效率,不利于生产。A、B、C组的幼虫死亡率与对照组无显著性差异,其转化时间分别为:10.9d,12.3d和15.5d,均比较适中,可以作为优选比例。
③综上所述,由于C组(鲜蓝藻藻泥:麸皮=3:1(蓝藻藻泥:麸皮干重比=1:7.5))具有较好的物料减少率,同时其转化率、转化时间和幼虫死亡率基本符合生产要求,可以选做物料转化的较佳比例。
实施例3:
亮斑扁角水虻幼虫在转化蓝藻藻泥的应用:
步骤如下:
①蓝藻藻泥(主要为水华微囊藻,Microcystisflos-aquae)含水率95.0%,定量玉米皮(含水率11%)量为100g,加入不同量的蓝藻藻泥,同时加入50g金针菇菌渣(含水率为52%),最后调节含水率在70%左右,其比例设置如下:
表5水虻幼虫转化蓝藻藻泥和玉米皮复配的配方组成
注:表中麸皮量、蓝藻藻泥量均为鲜重
②每个桶加入混合饲料后,再加入400头水虻幼虫(8日龄),任其自由取食,每组4个重复。控制室温28±2℃,环境湿度40%-60%。
③待出现50%的预蛹后,虫料分离。
④分别统计每组的50%预蛹时间、物料减少率、水虻转化率、幼虫死亡率。
⑤结果的统计与分析。
表6蓝藻藻泥和玉米皮不同配比下水虻转化效率评价
注:表中数据为上次重复平均值±标准误,数据之间进行单因素方差分析,同行数据的不同小写字母表示在0.05水平上差异性显著。
①随着蓝藻藻泥量的增加,幼虫死亡率和水虻转化率均无显著性差异,但物料减少率整随着蓝藻藻泥量的增加先上升,后减少,其中B1组具有最高的物料减少率,但水虻转化率最低;C1水虻转化率最高,但死亡率偏高,转化时间偏长(15.5天)A1虽然物料减少率比B1稍低,但水虻转化率比B1高,幼虫死亡率比B1低,预蛹时间预B1接近。
②随着蓝藻藻泥量的增加,50%预蛹时间呈先缩短后延长的现象,这可能是由于蓝藻藻泥中含有丰富的蛋白质,能提供水虻幼虫生长必须的营养,但由于蓝藻藻泥中含藻毒素,蓝藻藻泥含量过高反而会对水虻幼虫的生长有害,其中B1组转化周期最短,A1其次。
③从以上水虻转化蓝藻藻泥的参数综合考虑,B1组和A1组都可以作为蓝藻藻泥水虻转化的配方。
Claims (6)
1.亮斑扁角水虻在转化蓝藻藻泥同时降解藻毒素中的应用。
2.根据权利要求1所述的应用,其特征在于:将亮斑扁角水虻添加至含有蓝藻藻泥的基质中进行转化。
3.根据权利要求2所述的应用,其特征在于:所述的基质含有除蓝藻藻泥的其他辅料;所述的辅料为:
1)麸皮;或2)玉米皮和菌渣。
4.根据权利要求2所述的应用,其特征在于:蓝藻藻泥:麸皮=1:5-10;或蓝藻藻泥:玉米皮:菌渣=1:7-20:1-3;以上所述的比例均为干重比。
5.根据权利要求2所述的应用,其特征在于:所述的基质的含水率为65-85%。
6.根据权利要求1所述的应用,其特征在于:所述的亮斑扁角水虻为亮斑扁角水虻武汉品系的8日龄水虻幼虫。
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CN115777834A (zh) * | 2022-11-30 | 2023-03-14 | 鄂州市普罗欧博汇创业投资合伙企业(有限合伙) | 一种黑水虻粗蛋白饲料添加剂及其制备方法和应用 |
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