CN111000913A

CN111000913A - Traditional Chinese medicine composition for treating psoriasis

Info

Publication number: CN111000913A
Application number: CN201910588043.9A
Authority: CN
Inventors: 胡海峰; 李彻; 刘�英; 朱薇; 姜锦锦
Original assignee: Sinopharm Health Industry Institute Co ltd
Current assignee: Sinopharm Health Industry Institute Co ltd
Priority date: 2018-10-08
Filing date: 2019-07-02
Publication date: 2020-04-14
Also published as: CN109125443A

Abstract

The invention relates to a traditional Chinese medicine composition for treating psoriasis and a preparation thereof, wherein the traditional Chinese medicine composition comprises the following traditional Chinese medicine components: six Chinese herbal medicines such as garden burnet, lithospermum, madder, tree peony bark, angelica dahurica, liquorice and the like; the composition is extracted by alcohol to obtain alcohol extract, and the extract is added with corresponding pharmaceutical or cosmetic auxiliary materials to prepare preparation products such as hydrogel, emulsion, ointment and the like, and has the effect of remarkably treating psoriasis of mice. The Chinese medicinal compound composition for treating psoriasis has good activity for treating psoriasis, and can be applied to the treatment of psoriasis.

Description

Traditional Chinese medicine composition for treating psoriasis

Technical Field

The invention relates to the field of cosmetics and medicines, in particular to the field of prevention and treatment of psoriasis.

Background

Psoriasis is commonly called as psoriasis, is a chronic inflammatory skin disease, has long course of disease and easy recurrence tendency, and some cases are not cured almost for the whole life. The disease is mainly developed in young and old years, and has great influence on the physical health and mental conditions of patients. The clinical manifestations mainly include erythema and scales, all diseases occur in the whole body, the scalp and limbs are more common, and the symptoms are aggravated in winter. Psoriasis vulgaris is the most common type I, and is frequently attacked acutely; the typical manifestations are erythema with clear border and different shapes and sizes, and inflammatory halos around; slightly infiltrating and thickening; covering a plurality of layers of silvery scales on the surface; the scales are easy to scrape off, and after scraping, the scales are reddish and shiny, and a small bleeding point (Auspitz character) can be seen when the scales are scraped off. Psoriasis is also known as psoriatic arthritis. Patients with psoriasis suffer rheumatoid arthritis-like joint damage, which can affect the large and small joints of the whole body, but the disease is most characterized by the joint lesion between the distal finger (toe) joints; the affected joint is red, swollen and painful, and the skin around the joint is also red and swollen; the joint symptoms are usually aggravated or alleviated simultaneously with the skin symptoms, and the blood rheumatoid factor is negative. The externally applied medicine for treating psoriasis comprises:

(1) vitamin D3 analogs. The medicine comprises calcipotriol, tacalcitol and the like, and has good curative effect on plaque psoriasis. The composition can be used in combination with glucocorticoid or UVB to improve therapeutic effect. It should be used with cautions for patients with bone diseases, calcium metabolism disorder and renal insufficiency to avoid hypercalcemia. (2) A glucocorticoid. Topical glucocorticoids remain the current therapy commonly used to treat psoriasis. The strong hormone is suitable for the head and palms and soles, while the weak hormone is suitable for the face and the wiping area. Ointments and creams are commonly used for the general part. The head is applied with solution (propylene glycol) and gel. (3) Anthralin. It is commonly used for chronic plaque psoriasis. Can be made into ointment, paste and paraffin. The common concentration is 0.05-1.0%, and the concentration is gradually increased from low concentration according to the tolerance condition of patients. (4) Tretinoin. Gels and creams (0.05-0.1%) are effective against psoriasis when applied externally 1 or 2 times a day. Because of its slow onset of action, it is generally not used alone as a first-line drug. Can be used in combination with glucocorticoid such as clobetasol propionate, and the like, and the tazarotene is continuously applied after the skin damage is controlled, and the glucocorticoid is gradually stopped. Pregnant women, women in lactation and recent childbearing period are forbidden. (5) Tar oils. The common tar comprises coal tar, pine tar oil, bran distillate oil, black soybean distillate oil, etc., and is formulated into ointment with concentration of 5% for external use. The coal tar has good curative effect on chronic stable psoriasis, scalp psoriasis and palmoplantar psoriasis. Is not suitable for pregnant women, psoriasis of pustule type and erythrodermic type. The soluble coal tar can be used for bathing, and the coal tar shampoo is used for washing hair. The spirit of coal tar is used for applying and is effective in treating psoriasis on head. (6) Immunosuppressant and other external drugs. Such as tacrolimus, pimecrolimus external treatment and package treatment for intractable localized psoriasis. Camptothecin ointment 0.03%, salicylic acid ointment 5%, etc. Can also be used for treating psoriasis with Chinese medicinal materials, compound indigo naturalis pill, radix Tripterygii Wilfordii, compound Saviae Miltiorrhizae radix tablet, etc.

Some Chinese medicinal materials have various anti-inflammatory active ingredients, show strong anti-inflammatory activity in vivo and in vitro, and particularly show synergistic anti-inflammatory activity of Chinese medicinal compound materials such as sanguisorba, lithospermum, moutan bark, madder, angelica dahurica, liquorice and the like. These anti-inflammatory herbs are briefly introduced below:

sanguisorba officinalis (Latin scientific name: Sanguisorba officinalis L.) is a perennial herb of Sanguisorba of Rosaceae, and has spindle-shaped thick and strong heels, petiole brachypodium, mauve petals and fruit occluded in a calyx tube. Also named as Huangzhuyang, Yuzhang, Yufermented soya beans or Haematitum. Sanguisorba root, a little cold in nature and bitter in taste, is used as a medicine. The modern medical research proves that the garden burnet has the functions of stopping bleeding, cooling blood, clearing away heat and toxic materials, astringing to arrest diarrhea, resisting inflammation and inhibiting various pathogenic microorganisms, and can treat diseases such as hematemesis, bloody flux, burn, eczema, upper gastrointestinal hemorrhage, ulcer hemorrhage, hematochezia, metrorrhagia, tuberculous abscess, chronic osteomyelitis and the like. The sanguisorba contains about 17% of tannin and 2.5-4.0% of triterpenoid saponin. The saponins separated out are as follows: sanguisorba glucoside I, which is hydrolyzed to generate pomolic acid, arabinose and glucose; sanguisorba glucoside II is hydrolyzed to generate pomolic acid and arabinose; diospyros kaki saponin B is the triterpenoid saponin of glucuronic acid through preliminary identification. The effective component of the anti-inflammatory effect of the theine is 3, 3', 4-tri-O-methyl gallic acid, 4mg of tannin is applied to the ear of the mouse by ground transfusion, thus obviously inhibiting the croton oil induced auricle swelling, and the compound is orally taken for 1 g/(kg. d) and continuously 4d, thus effectively inhibiting the croton oil induced auricle swelling.

The other names of the lithospermum are as follows: radix Arnebiae, and radix Arnebiae. Is a Chinese medicinal herb recorded in Chinese pharmacopoeia, and the medicinal source is dry root of arnebia euchroma (Royle) Johnst, Lithospermum erythrorhizon or Lithospermum nakai of Boraginaceae. Shi Zi Cao is sweet, salty and cold in nature. It enters heart and liver meridians. Has the functions of cooling blood, promoting blood circulation, detoxifying and promoting eruption. The ethyl ether, ethanol or water extract of radix Arnebiae, alkannin, acetylalkannin, etc. can be administered orally or topically to inhibit hyperfunction of capillary permeability (including those caused by histamine, bromelain, bradykinin, and anti-rat serum and rabbit serum), and also can inhibit local edema of animal or peritonitis of mice caused by formaldehyde caused by carrageenan, anti-rat serum, rabbit serum, formaldehyde and heat stimulation. And inhibiting subcutaneous cotton ball granuloma hyperplasia, which shows that the composition has antagonism on vascular hyperpermeability, exudation and edema in acute exudation stage of inflammation and inflammation in proliferation stage. The removal of adrenal gland still has anti-inflammatory effect.

Moutan bark, cortex moutan, Chinese medicine name. Is dried root bark of Paeonia suffruticosa Andr. It is mostly distributed in Anhui, Sichuan, Gansu, Shaanxi, Hubei, Hunan, Shandong, Guizhou, etc. It is hard and brittle, and is easy to break, and its section is relatively flat, light pink and powdery. Fragrant smell, slightly bitter and astringent taste. Digging root in autumn, removing fine root, peeling root bark, and sun drying. Unprocessed or stir-baked. Compendium of materia Medica: nourishing yin to reduce pathogenic fire, removing speckle and toxic materials, relieving sore throat, and promoting urination. Later people only use Huang Ji to treat inflammation, but not know that the action of Dan Pi is better. Those with red flower are good, those with white flower are tonified, and those with white flower are rarely comprehended. Modern researches show that paeonol and other glycoside components have anti-inflammatory effect; the methanol extract of cortex moutan has platelet inhibiting effect; paeonol has central inhibitory effect on tranquilizing mind, lowering temperature, relieving fever, relieving pain, relieving spasm, etc., and has effects of resisting atherosclerosis, diuresis, and ulcer.

Madder (scientific name: Rubia cordifolia L.): rubiaceae, Rubia perennial grass climbing vine. Rubia cordifolia, being cold in nature and entering blood system, can cool blood and stop bleeding, and resolve blood stasis. It is indicated for bleeding due to recklessly blood flow due to blood heat. Cool blood and activate blood, dispel stasis, dredge meridians. Can be used for treating hematemesis, epistaxis, metrorrhagia, traumatic hemorrhage, amenorrhea, blood stasis, arthralgia, and traumatic injury with swelling and pain. Cooling blood to stop bleeding, promoting blood circulation to remove blood stasis: it can stop bleeding without retaining stasis, and is indicated for bleeding due to heat syndrome, amenorrhea and abdominal pain, and traumatic injury. The cuttlefish bone has stronger haemostasis.

The root of the dahurian angelica root is conical, the length of the dahurian angelica root is 10-20 cm, and the diameter of the dahurian angelica root is 2-2.5 cm. The surface is gray brown, and has transverse protruded skin holes and concave stem marks at the top. Hard, white cross section, enough powder, and densely covered brown oil spots on the skin. The nature and taste are warm, pungent, fragrant and slightly bitter; the functions mainly comprise expelling wind-damp, promoting blood circulation and expelling pus, promoting granulation and relieving pain. Can be used for treating headache, toothache, nasosinusitis, hemorrhoid, leucorrhea with red and white discharge, carbuncle, cellulitis, pyocutaneous disease, and skin pruritus.

Licorice (scientific name: Glycyrrhiza uralensis Fisch) alternative name: radix Angelicae Dahuricae, radix Glycyrrhizae, and radix Glycyrrhizae. Leguminous and glycyrrhiza herbs belong to perennial herbs, and the roots and the rhizomes are strong, so the Chinese herbal medicine is a tonifying Chinese herbal medicine. Has antiinflammatory, antiviral, liver protecting, toxic materials clearing away, and immunity enhancing effects. Because glycyrrhizic acid has glucocorticoid-like pharmacological action and no serious adverse reaction, the glycyrrhizic acid is widely used for clinically treating various acute and chronic hepatitis, bronchitis and AIDS, and also has the functions of resisting and preventing cancers, an interferon inducer, a cellular immunomodulator and the like. Glycyrrhizic acid has effects of reducing blood lipid and resisting atherosclerosis, and can prevent atherosclerosis. Licorice is the most commonly used medicine in clinic. The raw licorice can clear away heat and toxic materials, moisten lung and relieve cough, and harmonize the property of the medicines; prepared licorice root, radix Glycyrrhizae Praeparata has the effect of tonifying spleen and qi.

Chinese patent CN102727826A discloses a Chinese medicinal granule for treating psoriasis and a preparation process thereof, wherein a Chinese medicinal extract is prepared from a compound composition of Chinese medicinal materials such as honeysuckle flower, abalone shell, tree peony bark, dyers woad leaf, long pepper, spreading hedyotis herb, rehmannia root and turmeric by a secondary boiling water extraction method, and the Chinese medicinal granule is further prepared into the Chinese medicinal granule for treating psoriasis and has better effect of treating psoriasis. Chinese patent CN103655814B discloses a Chinese medicinal composition for treating psoriasis and application thereof, and also adopts oil extracts of three herbal compositions of lithospermum, madder, liquorice and the like to have the effect of treating psoriasis. Chinese patent CN106924378 discloses a lipstick for preventing or/and treating chronic cheilitis, which is prepared from a composition of three herbs including alkanna tinctoria, madder root, licorice root and the like by a secondary boiling water extraction method to prepare traditional Chinese medicine extracts, and further preparing a medicinal lipstick preparation with anti-inflammatory activity. Has better effect of treating chronic cheilitis.

The existing animal models are started with the three pathological links, and simulate partial pathological characteristics of psoriasis at different angles and levels, wherein a mouse psoriasis model induced by imiquimod is common, the imiquimod is a TLR7 agonist and an 8 agonist, can be combined with BALB/c mouse epidermal plasmacytes pDC and TLR7 in macrophages, secretes factors such as IFN- α, IL-23, IL-17 and the like, induces the epidermis to have histopathological changes such as hyperkeratosis and parakeratosis, thick spinous layer, inflammatory cell infiltration and the like, and is suitable for evaluating the effect of psoriasis treatment medicines.

The optimal formula is obtained by optimizing and screening multiple Chinese herbal medicine compounds, the optimal formula has the optimal effect of treating psoriasis, the composite anti-inflammatory activity of six Chinese herbal medicines of the garden burnet, the lithospermum, the moutan bark, the madder, the angelica dahurica, the liquorice and the like in the optimal Chinese herbal medicine composition screened according to the psoriasis model is stronger than the anti-inflammatory activity of a single medicinal material and stronger than the anti-inflammatory activity of three herbal medicine compositions of the lithospermum, the madder, the liquorice and the like, the remarkable advantage of multi-component synergistic anti-inflammatory of the Chinese herbal medicine is reflected, and the compound Chinese herbal medicine has.

Disclosure of Invention

In order to improve the effect of Chinese herbal medicines for treating psoriasis in the prior art and further optimize the traditional Chinese medicine compound for treating psoriasis, which is reported in the prior literature and takes the lithospermum, the madder and the liquorice as main components, the inventor discovers a new composition through a large number of animal experiments, has better synergistic effect, can improve the anti-inflammatory activity of the traditional Chinese medicine composition, and can also improve the effect of the traditional Chinese medicine composition for treating psoriasis. The traditional Chinese medicine raw materials used in the invention are all traditional Chinese medicine materials with small toxic and side effects, which are widely used clinically, and the product has good safety and can be used for a long time.

The invention provides a traditional Chinese medicine composition for treating psoriasis, which comprises the following components in percentage by mass: 10-45% of garden burnet root, 10-45% of lithospermum, 5-25% of madder root, 0-25% of tree peony bark, 0-20% of angelica dahurica and 5-25% of liquorice.

Preferably, the composition comprises the following components in percentage by mass: 20-40% of garden burnet, 20-40% of lithospermum, 10-25% of madder, 0-10% of moutan bark, 0-10% of angelica dahurica and 10-20% of liquorice.

Most preferably, the composition comprises the following components in percentage by mass: 25-30% of garden burnet, 25-30% of lithospermum, 15-20% of madder, 5-10% of moutan bark, 0-5% of angelica dahurica and 15-20% of liquorice.

The invention also provides a traditional Chinese medicine extract for treating psoriasis, which is prepared by the following method: weighing 1 part of any one of the traditional Chinese medicine compositions, adding 10 times of alcohol solvent, soaking for 12 hours, heating to 80 ℃, and performing reflux extraction for 2 hours; filtering, adding 10 times of alcohol solvent into the residue, heating and reflux extracting for 2 hr; filtering, mixing the two ethanol solvent extracts, and concentrating under reduced pressure in 50 deg.C water bath to obtain pulp.

The alcohol solvent comprises small molecular aliphatic alcohols such as methanol, ethanol, isopropanol and the like, and ethanol is preferred.

The invention also provides an external plaster preparation for treating psoriasis, which is characterized in that the plaster is prepared by the following method: 1) weighing 1 part of synthetic wax, 3 parts of ozokerite and 3 parts of synthetic beeswax, respectively heating in a water bath at 80 ℃, stirring, dissolving, and then mixing to obtain solution A;

2) weighing BHT0.5 parts, lanolin 2.5 parts, and white vaseline 2.5 parts, heating in 80 deg.C water bath, stirring for dissolving, adding solution A, stirring, and mixing to obtain solution B

3) Weighing 0.5 part of caprylic triglyceride, 14 parts of the traditional Chinese medicine extract for treating psoriasis, 2 parts of glycerol and 1 part of essence, heating in a water bath at 80 ℃, stirring and mixing uniformly, adding the solution B, mixing and stirring uniformly to obtain a solution C;

4) and (4) introducing the solution C into a glass bottle or other metal tubes while the solution C is hot, and cooling to obtain a plaster preparation product.

The invention also provides an external hydrogel preparation for treating psoriasis, which is characterized by being prepared by the following method:

1) weighing 0.3 part of carbomer 940, adding 4.0 parts of deionized water, stirring and soaking for 6-12 hours, and uniformly mixing to obtain a carbomer solution A;

2) weighing 2 parts of the traditional Chinese medicine extract for treating psoriasis, 1.5 parts of glycerol and 1.5 parts of absolute ethyl alcohol, heating in a water bath at 60 ℃, stirring, dissolving, adding the solution A, stirring and mixing uniformly to obtain solution B;

3) weighing 0.5 part of azone, 0.2 part of allantoin and 0.5 part of essence, stirring and mixing uniformly at normal temperature, adding the solution B, mixing and stirring uniformly, and homogenizing uniformly to obtain a hydrogel C solution;

4) weighing 0.1 part of triethylamine solution, slowly dropwise adding the triethylamine solution into the solution C while stirring, and adjusting the viscosity of the hydrogel to 20 pas to obtain the external hydrogel preparation.

The traditional Chinese medicine composition for treating psoriasis and the preparation thereof can be prepared into common cosmetics, medicinal cosmetics and traditional Chinese medicine compound external preparations.

The reagents and starting materials used in the present invention are commercially available.

The sanguisorba comprises a sanguisorba root medicinal material, a traditional Chinese medicine decoction piece, sanguisorba root powder or an extract thereof; the radix Arnebiae is selected from radix Arnebiae medicinal material, Chinese medicinal decoction pieces, and herba Arnebiae powder or its extract; the cortex moutan comprises cortex moutan traditional Chinese medicinal materials, Chinese medicinal decoction pieces, cortex moutan powder and extracts thereof; the madder is a whole madder comprising traditional Chinese medicinal materials, traditional Chinese medicine decoction pieces and an extract; the radix Angelicae Dahuricae is radix Angelicae Dahuricae including radix Angelicae Dahuricae Chinese medicinal material, Chinese medicinal decoction pieces, radix Angelicae Dahuricae root powder and its extract; the Glycyrrhrizae radix is Glycyrrhrizae radix including Glycyrrhrizae radix Chinese medicinal material, Chinese medicinal decoction pieces, Glycyrrhrizae radix powder and its extract.

The positive progress effects of the invention are as follows: the traditional Chinese medicine composition for preventing or treating psoriasis provided by the invention is rationally designed, and has a large amount of data screened by animal experiments, so that the synergistic effect of the six medicinal materials is good; the medicinal materials are all nontoxic medicinal materials, so that the safety is good, the formula is simpler, the quality control is easy, and the key point is that the more excellent anti-inflammatory effect can be achieved.

Detailed Description

The present invention is further illustrated by the following examples, which are not intended to limit the scope of the invention, and the experimental procedures not described in the following examples are all conventional experimental procedures, and are performed in accordance with the commercial specifications.

The formula of the traditional Chinese medicine compound composition for treating psoriasis comprises the following steps:

TABLE 1 formulations of examples 3-8

Example numbering	Radix Sanguisorbae	Lithospermum erythrorhizon	Cortex moutan	Root of India madder	Root of Dahurian Angelica	Licorice root, radix Glycyrrhizae	Kind of alcohol
								3	10	45	0	25	0	20	Methanol
4	20	30	10	15	0	25	Ethanol
								5	30	20	20	10	10	10	Ethanol
6	20	30	20	10	10	10	Ethanol
								7	45	10	10	5	20	10	Propanol(s)
8	45	10	25	5	10	5	Methanol

Example 1:

according to the Chinese patent CN106924378, 40 g of lithospermum, 20g of madder and 20g of liquorice are weighed, and are added with 2400ml of water (30 times) to be soaked in boiling water for extraction for 2 hours; vacuum filtering, extracting the residue with 1600ml boiling water for 2 hr, filtering, mixing the two supernatants, and concentrating under reduced pressure at 60 deg.C to density of 1.10g/cm³Thus obtaining the extract of the Chinese medicinal composition.

Example 2:

according to the Chinese patent CN103655814B, weighing 32 g of lithospermum, 32 g of madder and 28 g of liquorice, adding 600g of olive oil, soaking for 48 hours, then frying for 8 hours with slow fire, filtering dregs of a decoction, and cooling the oil formula to obtain the extract of the traditional Chinese medicine compound.

Example 3:

weighing 10g of garden burnet, 45 g of lithospermum, 0g of moutan bark, 25 g of madder, 0g of angelica dahurica and 20g of liquorice according to the table 1 respectively, adding 1000ml (10 times) of methanol, soaking for 12 hours, and heating to 80 ℃ for extraction for 2 hours; filtering, adding 1000ml (10 times) of methanol into the residue, and heating and extracting for 2 hours; filtering, and mixing the two methanol extracts to obtain methanol extract of Chinese medicinal composition; concentrating under reduced pressure in 50 deg.C water bath to obtain pulp to obtain Chinese medicinal extract.

Example 4:

weighing 20g of garden burnet, 30 g of lithospermum, 10g of moutan bark, 15 g of madder, 0g of angelica dahurica and 25 g of liquorice respectively according to the table 1, adding 1000ml (10 times) of ethanol, soaking for 12 hours, and heating to 80 ℃ for extraction for 2 hours; filtering, adding 1000ml (10 times) of ethanol into the residue, and heating and extracting for 2 hours; filtering, and mixing the secondary ethanol extracts to obtain ethanol extract of Chinese medicinal composition; concentrating under reduced pressure in 50 deg.C water bath to obtain pulp to obtain Chinese medicinal extract.

Example 5:

weighing 30 g of garden burnet, 20g of lithospermum, 20g of moutan bark, 10g of madder, 10g of angelica dahurica and 10g of liquorice respectively according to the table 1, adding 1000ml (10 times) of ethanol, soaking for 12 hours, and heating to 80 ℃ for extraction for 2 hours; filtering, adding 1000ml (10 times) of ethanol into the residue, and heating and extracting for 2 hours; filtering, and mixing the secondary ethanol extracts to obtain ethanol extract of Chinese medicinal composition; concentrating under reduced pressure in 50 deg.C water bath to obtain pulp to obtain Chinese medicinal extract.

Example 6:

weighing 20g of garden burnet, 30 g of lithospermum, 20g of moutan bark, 10g of madder, 10g of angelica dahurica and 10g of liquorice respectively according to the table 1, adding 1000ml (10 times) of ethanol, soaking for 12 hours, and heating to 80 ℃ for extraction for 2 hours; filtering, adding 1000ml (10 times) of ethanol into the residue, and heating and extracting for 2 hours; filtering, and mixing the secondary ethanol extracts to obtain ethanol extract of Chinese medicinal composition; concentrating under reduced pressure in 50 deg.C water bath to obtain pulp to obtain Chinese medicinal extract.

Example 7

Weighing 45 g of garden burnet, 10g of lithospermum, 10g of moutan bark, 5 g of madder, 20g of angelica dahurica and 10g of liquorice respectively according to the table 1, adding 1000ml (10 times) of ethanol, soaking for 12 hours, heating to 80 ℃ and extracting for 2 hours; filtering, adding 1000ml (10 times) of ethanol into the residue, and heating and extracting for 2 hours; filtering, and mixing the secondary ethanol extracts to obtain ethanol extract of Chinese medicinal composition; concentrating under reduced pressure in 50 deg.C water bath to obtain pulp to obtain Chinese medicinal extract.

Example 8

Weighing 45 g of garden burnet, 10g of lithospermum, 25 g of moutan bark, 5 g of madder, 10g of angelica dahurica and 5 g of liquorice respectively according to the table 1, adding 1000ml (10 times) of ethanol, soaking for 12 hours, heating to 80 ℃ and extracting for 2 hours; filtering, adding 1000ml (10 times) of ethanol into the residue, and heating and extracting for 2 hours; filtering, and mixing the secondary ethanol extracts to obtain ethanol extract of Chinese medicinal composition; concentrating under reduced pressure in 50 deg.C water bath to obtain pulp to obtain Chinese medicinal extract.

Example 9: preparation of plaster

Weighing 1 part of synthetic wax, 3 parts of ozokerite and 3 parts of synthetic beeswax, respectively heating in a water bath at 80 ℃, stirring, dissolving, and mixing to obtain solution A; weighing 0.5 part of BHT (2, 6-di-tert-butyl-p-cresol), 2.5 parts of lanolin and 2.5 parts of white vaseline, heating in a water bath at 80 ℃, stirring, dissolving, adding the solution A, and uniformly stirring and mixing to obtain solution B; weighing 0.5 part of caprylic triglyceride, 14 parts of Chinese medicinal extract, 2 parts of glycerol and 1 part of essence, heating in a water bath at 80 ℃, stirring and mixing uniformly, adding the solution B, mixing and stirring uniformly to obtain a solution C; and (4) introducing the solution C into a tube while the solution C is hot to prepare the plaster preparation.

Example 10: preparation of hydrogel formulations

2) weighing 2 parts of traditional Chinese medicine extract, 1.5 parts of glycerol and 1.5 parts of absolute ethyl alcohol, heating in a water bath at 60 ℃, stirring and dissolving, adding the solution A, stirring and mixing uniformly to obtain solution B;

Example 11: psoriasis treatment effect experiment

The following mouse psoriasis model efficacy evaluation was performed on the Chinese medicinal compositions prepared in examples 1 to 8 by mouse test.

Medicaments and agents

Imiquimod cream, halomethasone (positive control), are all commercially available.

The test composition, using the extracts (extractum) of the chinese traditional medicine prepared in examples 1 to 8, was prepared into a plaster or hydrogel formulation according to the method of example 9 or 10, specifically:

product 1: a hydrogel preparation prepared from the extract of the Chinese medicinal material of example 1;

2-3 of products: plaster preparations prepared from the extracts of the Chinese herbs of examples 2 and 3, respectively;

4-8 parts of product: hydrogel formulations were prepared using the herbal extracts of examples 4-8, respectively.

Laboratory animal

BALB/c mice, SPF grade, males, weighing around 22 g. The experimental animal is provided by Shanghai Sphere-BiKai experimental animals Co., Ltd, and the animal production license number: SCXK (Shanghai) 2013-0016. 10 animals are raised in each cage, the room temperature of the animals is set to be 20-22 ℃, the humidity is set to be 40% -70%, and the animals are alternately illuminated in light and shade for 12 hours. The padding is replaced at least 2 times per week, the feeding box is replaced at the same time, and the feeding box is replaced at any time when abnormal conditions occur.

Preparing an animal model: BALB/c mice were anesthetized by intraperitoneal injection with 1% chloral hydrate (0.2ml/20g), their back hairs were shaved off to form an exposed area of 2cm by 3cm in size, and the mice were housed in individual cages for 1d and then treated with drugs in groups.

Experimental methods

1) Animal grouping: the mice were randomly divided into 10 groups, which were 10 groups, i.e., a model group, a halometasone group (positive drug), a product 1 group, a product 2 group, a product 3 group, a product 4 group, a product 5 group, a product 6 group, a product 7 group, and a product 8 group, respectively.

2) Dosage and method of administration:

normal group: not processing;

model group: mice were regularly coated with imiquimod on their bare backs in the morning 1 time a day. Smearing blank matrix in the afternoon, twice a day for 8 consecutive days, and the administration dosage is shown in Table 3;

halometasone group: mice were regularly coated with imiquimod on their bare backs in the morning 1 time a day. The positive drug halometasone is applied and administered at regular time in the afternoon, 2 times a day, and the administration is continuously performed for 8 days, and the administration dosage is shown in table 3;

products 1-8 dosing groups: mice were regularly coated with imiquimod on their bare backs in the morning 1 time a day. The corresponding product is applied by smearing at fixed time in the afternoon for 2 times a day for 8 days, and the administration dosage is shown in table 3;

TABLE 3 dosage of administration

Imiquimod	Halometasone	1-8 products
			20mg/cm²	10mg/cm²	0.238g/cm²

3) The detection indexes and the method are as follows:

① according to the evaluation standard of psoriasis skin lesion area and disease severity (PASI) of each group of mice, the integration of corresponding erythema, scale and infiltration thickening degree is given to the mice, the integration of the three is added to obtain the total integration, the change condition of the skin lesion of the mice is observed, and the evaluation standard is shown in the following table 4.

TABLE 4 psoriasis lesion severity score

② cutting back skin of each group of mice, slicing, HE staining, taking pictures under 20 Xobjective lens, and measuring and counting thickness of three parts of epidermis by ImageJ (1.49V, NIH) picture processing software;

results of the experiment

TABLE 4 psoriasis lesion severity score

Group of	Thickening of soil	Erythema	Scale and chip	Total score	Cortex thickness (μm)
						Normal group	0	0	0	0	18.03±2.32
Model set	2.25±0.75	1.50±0.5	2.50±0.5	6.25±0.75	99.33±10.37
						Halometasone group	0	0.6±0.4	1.8±0.6	2.4±0.8	59.89±5.71
Product 1 group	0.25±0.75	1.25±0.75	1.5±0.4	3.0±0.8	47.05±3.12
						Product 2 group	0.2±0.7	1.8±0.7	1.4±0.7	3.4±0.9	60.99±6.77
Product 3 groups	0.5±0.2	0	0	0.5±0.2	52.28±4.10
						Product 4 groups	0.2±0.1	0.5±0.3	0	0.7±0.3	43.57±3.44
Product 5 groups	0.1±0.1	0.3±0.2	1.0±0.4	1.4±0.6	60.10±5.31
						Product 6 groups	0	0.5±0.3	0	0.5±0.2	55.76±4.71
Product 7 groups	0.1±0.1	0.3±0.1	0.5±0.2	0.8±0.2	43.57±2.18
						Product 8 groups	0	0.3±0.2	0.5±0.1	0.8±0.4	46.84±3.40

According to the experimental data, the product 1-2 groups are control groups, and the product 3-8 groups show stronger psoriasis treatment efficacy than the control groups, so that the six-ingredient traditional Chinese medicine compound composition has better effect than the three-herb composition, and has stronger psoriasis treatment efficacy.

Claims

1. A traditional Chinese medicine composition for treating psoriasis comprises the following components in percentage by mass: 10-45% of garden burnet root, 10-45% of lithospermum, 5-25% of madder root, 0-25% of tree peony bark, 0-20% of angelica dahurica and 5-25% of liquorice.

2. The traditional Chinese medicine composition for treating psoriasis according to claim 1 comprises the following components in percentage by mass: 20-40% of garden burnet, 20-40% of lithospermum, 10-25% of madder, 0-10% of moutan bark, 0-10% of angelica dahurica and 10-20% of liquorice.

3. The traditional Chinese medicine composition for treating psoriasis according to claim 1, which comprises the following components in percentage by mass: 25-30% of garden burnet, 25-30% of lithospermum, 15-20% of madder, 5-10% of moutan bark, 0-5% of angelica dahurica and 15-20% of liquorice.

4. The traditional Chinese medicine extract for treating psoriasis is characterized by being prepared by the following method: soaking the Chinese medicinal composition of any one of claims 1-3 in 10 times of alcohol solvent for 12 hr, heating to 80 deg.C, and reflux-extracting for 2 hr; filtering, adding 10 times of alcohol solvent into the residue, heating and reflux extracting for 2 hr; filtering, mixing the two ethanol solvent extracts, and concentrating under reduced pressure in 50 deg.C water bath to obtain pulp.

5. The extract of Chinese herbs according to claim 4, wherein the alcohol solvent comprises small molecular aliphatic alcohols such as methanol, ethanol, isopropanol, etc., preferably ethanol.

6. Use of a Chinese medicinal composition or extract according to any one of claims 1 to 5 in the manufacture of a cosmetic or pharmaceutical preparation for the treatment of psoriasis.

7. The use of claim 6, wherein the formulation comprises a hydrogel, an emulsion, an ointment, or a plaster.

8. The use according to claim 6, wherein the pharmaceutical or cosmetic composition further comprises pharmaceutically or cosmetically acceptable adjuvants.

9. A plaster preparation for external use for treating psoriasis, which is characterized in that the plaster is prepared by the following method:

1) weighing 1 part of synthetic wax, 3 parts of ozokerite and 3 parts of synthetic beeswax, respectively heating in a water bath at 80 ℃, stirring, dissolving, and then mixing to obtain solution A;

2) weighing 0.5 part of BHT, 2.5 parts of lanolin and 2.5 parts of white vaseline, heating in a water bath at 80 ℃, stirring for dissolving, adding the solution A, and stirring and mixing uniformly to obtain solution B;

3) weighing 0.5 part of caprylic triglyceride, 14 parts of the traditional Chinese medicine extract as claimed in claim 4, 2 parts of glycerol and 1 part of essence, heating in a water bath at 80 ℃, stirring and mixing uniformly, adding the solution B, mixing and stirring uniformly to obtain a solution C;

4) and (4) introducing the solution C into a glass bottle or other metal tubes while the solution C is hot, and cooling to obtain a plaster preparation product. An external hydrogel formulation for treating psoriasis, wherein the hydrogel formulation is prepared by the following method:

2) weighing 2 parts of the traditional Chinese medicine extract of claim 4, 1.5 parts of glycerol and 1.5 parts of absolute ethyl alcohol, heating in a water bath at 60 ℃, stirring and dissolving, adding the solution A, stirring and mixing uniformly to obtain solution B;