CN110981950A - Paddy-potato collagen extraction equipment - Google Patents

Paddy-potato collagen extraction equipment Download PDF

Info

Publication number
CN110981950A
CN110981950A CN201911294437.XA CN201911294437A CN110981950A CN 110981950 A CN110981950 A CN 110981950A CN 201911294437 A CN201911294437 A CN 201911294437A CN 110981950 A CN110981950 A CN 110981950A
Authority
CN
China
Prior art keywords
collagen
liquid storage
liquid
storage bottle
filtering component
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201911294437.XA
Other languages
Chinese (zh)
Inventor
苏来金
徐仰丽
林胜利
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wenzhou Polytechnic
Original Assignee
Wenzhou Polytechnic
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wenzhou Polytechnic filed Critical Wenzhou Polytechnic
Priority to CN201911294437.XA priority Critical patent/CN110981950A/en
Publication of CN110981950A publication Critical patent/CN110981950A/en
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/78Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D36/00Filter circuits or combinations of filters with other separating devices

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention discloses a sea cucumber collagen extraction device, relates to the technical field of collagen extraction, and solves the problems that the method for extracting collagen from sea cucumbers in the prior art is complex in operation and is easy to infect mixed bacteria, and the technical key points are as follows: the device comprises a support frame, wherein a first liquid storage bottle and a second liquid storage bottle for temporarily storing extracting solution are arranged on the support frame, a first pressure pump for pressurizing during first filtration is arranged between the first liquid storage bottle and the second liquid storage bottle, a first filtering component and a second filtering component for filtering are connected below the second liquid storage bottle, and a second pressure pump for pressurizing during second filtration is arranged between the first filtering component and the second filtering component; the collagen extracting process is in a sealed state, and infection of infectious microbes by collagen is avoided.

Description

Paddy-potato collagen extraction equipment
Technical Field
The invention relates to the technical field of collagen extraction, in particular to a acaudina molpadioides collagen extraction device.
Background
The research shows that the acaudina molpadioides are rich in nutrient components, contain rich protein, polysaccharide, lower fat and amino acid, have extremely high contents of calcium, zinc and iron elements, and also contain selenium, vitamin A, vitamin B6, vitamin E and the like, wherein the content of crude protein on the body wall of the acaudina molpadioides accounts for 89.16 percent of the dry weight of the acaudina molpadioides and is higher than that of the crude protein of stichopus japonicus, the main components of the protein are active collagen, and if the low-value acaudina molpadioides is extracted and purified, the resources of the acaudina molpadioides can be fully utilized and the economic value of the acaudina molpadioides is improved.
Chinese patent publication No. CN103539855B discloses a method for extracting acaudina molpadioides collagen, which adopts fresh and frozen acaudina molpadioides or dry acaudina molpadioides as raw materials, and comprises the steps of cleaning and decontaminating the raw materials, combined peeling, decoloring, cutting into blocks, pulping, adjusting the liquid-material ratio, adjusting the pH, heating and hydrolyzing, crudely extracting, purifying, drying and the like, thereby extracting acaudina molpadioides collagen. The method removes heavy metal pollution before hydrolysis, does not use strong acid and strong alkali in the hydrolysis process, and is simple and convenient to operate. However, in the method, the collagen is extracted by multiple times of filtration, in the prior art, the filters need to be replaced for multiple times of filtration, the operation process is complex, and foreign bacteria are easily infected in the filter replacing process, so that the collagen is not extracted properly, and therefore, the acaudina molpadioides collagen extracting equipment is provided.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a acaudina molpadioides collagen extraction device, which comprises a support frame, wherein a first liquid storage bottle and a second liquid storage bottle for temporarily storing an extracting solution are arranged on the support frame, a first pressure pump for pressurizing during first filtration is arranged between the first liquid storage bottle and the second liquid storage bottle, a first filtering component and a second filtering component for filtering are connected below the second liquid storage bottle, and a second pressure pump for pressurizing during second filtration is arranged between the first filtering component and the second filtering component, so that the problems that the method for extracting collagen from acaudina molpadioides in the prior art is complex in operation and is easy to infect infectious microbes are solved.
In order to achieve the purpose, the invention provides the following technical scheme:
the utility model provides a sea cucumber collagen draws equipment, includes the support frame, is equipped with first stock solution bottle and the second stock solution bottle that is used for interim storage extract on the support frame, is equipped with the first force (forcing) pump that pressurizes when being used for filtering for the first time between first stock solution bottle and the second stock solution bottle, is connected with first filtering component and the second filtering component that is used for filtering under the second stock solution bottle, is equipped with the second force (forcing) pump that pressurizes when being used for filtering for the second time between first filtering component and the second filtering component.
As a further scheme of the invention, a pressure stabilizing pump is also arranged on the second liquid storage bottle and is communicated with the inside of the second liquid storage bottle through an air pipe, and the pressure stabilizing pump is connected with a nitrogen or inert gas source.
As a further scheme of the invention, the second liquid storage bottle is also provided with a pressure release valve which is communicated with the interior of the second liquid storage bottle through an air pipe.
As a further scheme of the invention, the first filtering component is formed by hermetically connecting an upper sealing shell and a lower sealing shell, a microfiltration membrane is arranged between the upper sealing shell and the lower sealing shell, liquid inlets are respectively arranged on the upper sealing shell and the lower sealing shell, the liquid inlets are hermetically connected with the upper sealing shell and the lower sealing shell, a step is arranged at the interface of the liquid inlets, a sealing cover is arranged on the liquid inlets, and the liquid inlets are connected with the liquid outlets of the second liquid storage bottles through threads.
As a further scheme of the invention, the second filtering component comprises a shell and an ultrafiltration membrane, the shell is provided with a cylindrical metal pipe in a sealing mode, the upper end of the ultrafiltration membrane is connected to a liquid outlet of the second pressure pump through the sealed metal pipe, and the lower end of the ultrafiltration membrane is provided with a protein outlet.
As a further proposal of the invention, the shell is provided with a waste liquid outlet.
As a further proposal of the invention, a filter screen is arranged at the liquid inlet of the first liquid storage bottle.
As a further scheme of the invention, the ultrafiltration membrane is communicated with an ethanol pump, and the ethanol pump is communicated with an ethanol storage tank.
As a further scheme of the invention, a sealing valve is also arranged between the ethanol pump and the ultrafiltration membrane.
In conclusion, compared with the prior art, the invention has the following beneficial effects:
compared with the prior art, the collagen extracting process is in a sealed state, the infection of foreign bacteria by collagen is avoided, the extracting steps are simplified, and the extracting time is saved.
Drawings
FIG. 1 is a schematic structural diagram of an embodiment 1 of a acaudina molpadioides collagen extraction apparatus.
Fig. 2 is a schematic structural diagram of a first filtering component in the acaudina molpadioides collagen extraction device.
Fig. 3 is a schematic structural diagram of the acaudina molpadioides collagen extraction device in embodiment 3.
Reference numerals: 1-support frame, 2-first liquid storage bottle, 3-second liquid storage bottle, 31-pressure stabilizing pump, 32-pressure release valve, 4-first pressure pump, 5-first filtering component, 51-upper sealing shell, 52-lower sealing shell, 53-liquid inlet, 54-sealing cover, 6-second filtering component, 61-shell, 62-ultrafiltration membrane, 63-protein outlet, 64-waste liquid outlet, 65-ethanol pump, 66-sealing valve and 7-second pressure pump.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
Example 1
As shown in fig. 1, a acaudina molpadioides collagen extraction device comprises a support frame 1, wherein a first liquid storage bottle 2 and a second liquid storage bottle 3 for temporarily storing an extracting solution are arranged on the support frame 1, a first pressure pump 4 for pressurizing during first filtration is arranged between the first liquid storage bottle 2 and the second liquid storage bottle 3, a first filtering component 5 and a second filtering component 6 for filtering are connected below the second liquid storage bottle 3, and a second pressure pump 7 for pressurizing during second filtration is arranged between the first filtering component 5 and the second filtering component 6;
the first liquid storage bottle 2 and the second liquid storage bottle 3 are both round glass bottles, a liquid inlet is arranged on the first liquid storage bottle 2, a liquid outlet is arranged below the first liquid storage bottle 2, the liquid outlet is communicated with the first pressure pump 4 through a pipe joint, the first pressure pump 4 is communicated with the second liquid storage bottle 3 through a glass pipe, and the first pressure pump 4 achieves the purpose of pressurizing and inputting liquid in the first liquid storage bottle 2 into the second liquid storage bottle 3;
preferably, in order to prevent the pressure in the second liquid storage bottle 3 from reducing, a pressure stabilizing pump 31 is further arranged on the second liquid storage bottle 3, the pressure stabilizing pump 31 is communicated with the inside of the second liquid storage bottle 3 through a gas pipe, and the pressure stabilizing pump 31 is connected with a nitrogen or inert gas source;
further, in order to prevent the pressure in the second liquid storage bottle 3 from being too high, a pressure release valve 32 is further arranged on the second liquid storage bottle 3, and the pressure release valve 32 is communicated with the interior of the second liquid storage bottle 3 through an air pipe;
the first filtering component 5 is a filter screen box, as shown in fig. 2, the first filtering component 5 is formed by hermetically connecting an upper sealing shell 51 and a lower sealing shell 52, a microfiltration membrane is arranged between the upper sealing shell 51 and the lower sealing shell 52, liquid inlets 53 are respectively arranged on the upper sealing shell 51 and the lower sealing shell 52, the liquid inlets 53 are hermetically connected with the upper sealing shell 51 and the lower sealing shell 52, a step is arranged at the interface of the liquid inlets 53, a sealing cover 54 is arranged on the liquid inlets 53, and the liquid inlets 53 are connected with the liquid outlets of the second liquid storage bottles 3 through threads for sealing the liquid inlets 53 and the liquid outlets of the second liquid storage bottles 3;
the second filtering component 6 is a modification of an ultrafilter in the prior art, the second filtering component 6 comprises a shell 61 and an ultrafiltration membrane 62, the shell 61 is a cylindrical metal tube, the shell 61 is sealed, the ultrafiltration membrane 62 is an 80kDa ultrafiltration membrane, the upper end of the ultrafiltration membrane 62 is connected to a liquid outlet of the second booster pump 7 through a sealed metal tube, and the lower end of the ultrafiltration membrane 62 is provided with a protein outlet 63 for collecting collagen solution, so that raw materials are provided for the next drying;
preferably, in order to facilitate the outflow of the filtered waste liquid, a waste liquid outlet 64 is provided on the housing 61, and a waste liquid collecting container is provided below the waste liquid outlet 64.
Example 2
As shown in fig. 1, a acaudina molpadioides collagen extraction device comprises a support frame 1, wherein a first liquid storage bottle 2 and a second liquid storage bottle 3 for temporarily storing an extracting solution are arranged on the support frame 1, a first pressure pump 4 for pressurizing during first filtration is arranged between the first liquid storage bottle 2 and the second liquid storage bottle 3, a first filtering component 5 and a second filtering component 6 for filtering are connected below the second liquid storage bottle 3, and a second pressure pump 7 for pressurizing during second filtration is arranged between the first filtering component 5 and the second filtering component 6;
further, the extraction step is simplified, and a filter screen with the aperture of 0.01mm is arranged on the liquid inlet of the first liquid storage bottle 2;
the rest is the same as the case support 1.
Example 3
As shown in fig. 2, a acaudina molpadioides collagen extraction device comprises a support frame 1, wherein a first liquid storage bottle 2 and a second liquid storage bottle 3 for temporarily storing an extracting solution are arranged on the support frame 1, a first pressure pump 4 for pressurizing during first filtration is arranged between the first liquid storage bottle 2 and the second liquid storage bottle 3, a first filtering component 5 and a second filtering component 6 for filtering are connected below the second liquid storage bottle 3, and a second pressure pump 7 for pressurizing during second filtration is arranged between the first filtering component 5 and the second filtering component 6;
further, in order to fully collect the collagen in the ultrafiltration membrane 62, the ultrafiltration membrane 62 is communicated with an ethanol pump 65, the ethanol pump 65 is communicated with an ethanol storage tank, and the ethanol pump 65 plays a role in washing the inside of the ultrafiltration membrane 62;
further, in order to prevent the liquid in the ultrafiltration membrane 62 from flowing out through the ethanol pump 65 after the second filtration module 6 is pressurized, a sealing valve 66 is further arranged between the ethanol pump 65 and the ultrafiltration membrane 62;
otherwise, the same as in example 2.
In summary, the working principle of the invention is as follows:
the centrifugal acaudina molpadioides crude extracting solution enters the first liquid storage bottle 2 from the first liquid storage bottle 2, is subjected to coarse filtration by a filter screen on the first liquid storage bottle 2, impurities in the crude extracting solution are removed, then the crude extracting solution is subjected to pressurization by the first pressure pump 4, pressure regulation by the pressure stabilizing pump 31 and the pressure relief valve 32, so that the pressure value of the first filtration in the second liquid storage bottle 3 is kept, the crude extracting solution is further subjected to impurity removal in the crude extracting solution through filtration of the first filtering component 5 to obtain an extracting solution, then ultrafiltration is performed by the second filtering component 6, moisture and particle impurities in the extracting solution are removed, a collagen solution is obtained, and collagen is obtained after drying.
It should be particularly noted that the collagen extraction principle in the present application is an application of the prior art, and the simplification of the collagen extraction step by a special device is an innovative point of the present application, which effectively solves the problems of complicated operation and easy infectious microbes of the method for extracting collagen from acaudina molpadioides in the prior art.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are only for the convenience of description of the invention and to simplify the description, and do not indicate or imply that the referred device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (9)

1. The utility model provides a sea cucumber collagen draws equipment, a serial communication port, including support frame (1), be equipped with first stock solution bottle (2) and second stock solution bottle (3) that are used for temporary storage extract on support frame (1), be equipped with between first stock solution bottle (2) and second stock solution bottle (3) and be used for first time pressurized first force (forcing) pump (4) when filtering, be connected with under second stock solution bottle (3) and be used for filterable first filtering component (5) and second filtering component (6), be equipped with between first filtering component (5) and second filtering component (6) and be used for the second time pressurized second force (forcing) pump (7) when filtering.
2. The acaudina molpadioides collagen extraction equipment according to claim 1, wherein the second liquid storage bottle (3) is further provided with a pressure stabilizing pump (31), the pressure stabilizing pump (31) is communicated with the inside of the second liquid storage bottle (3) through an air pipe, and the pressure stabilizing pump (31) is connected with a nitrogen or inert gas source.
3. The acaudina molpadioidea collagen extraction device according to claim 2, wherein the second liquid storage bottle (3) is further provided with a pressure release valve (32), and the pressure release valve (32) is communicated with the inside of the second liquid storage bottle (3) through an air pipe.
4. The extraction equipment of the acaudina molpadioides collagen protein as claimed in claim 1, wherein the first filtering component (5) is formed by hermetically connecting an upper sealed shell (51) and a lower sealed shell (52), a microfiltration membrane is arranged between the upper sealed shell (51) and the lower sealed shell (52), liquid inlets (53) are respectively arranged on the upper sealed shell (51) and the lower sealed shell (52), the liquid inlets (53) are hermetically connected with the upper sealed shell (51) and the lower sealed shell (52), a step is arranged at the interface of the liquid inlets (53), a sealing cover (54) is arranged on the liquid inlets (53), and the liquid inlets (53) are in threaded connection with the liquid outlets of the second liquid storage bottles (3).
5. The extraction equipment of the acaudina molpadioides collagen according to claim 1, wherein the second filtering component (6) comprises a casing (61) and an ultrafiltration membrane (62), the casing (61) is a cylindrical metal pipe, the casing (61) is hermetically arranged, the upper end of the ultrafiltration membrane (62) is connected to the liquid outlet of the second pressure pump (7) through the sealed metal pipe, and the lower end of the ultrafiltration membrane (62) is provided with a protein outlet (63).
6. The acaudina collagen extraction apparatus according to claim 5, wherein the housing (61) is provided with a waste liquid outlet (64).
7. The acaudina molpadioides collagen extracting apparatus as claimed in any one of claims 1 to 6, wherein the liquid inlet of the first liquid storage bottle (2) is provided with a filter screen.
8. The acaudina collagen extraction apparatus according to any one of claims 1 to 6, wherein the ultrafiltration membrane (62) is in communication with an ethanol pump (65), and the ethanol pump (65) is in communication with an ethanol storage tank.
9. The acaudina collagen extraction apparatus according to claim 8, wherein a sealing valve (66) is further provided between the ethanol pump (65) and the ultrafiltration membrane (62).
CN201911294437.XA 2019-12-16 2019-12-16 Paddy-potato collagen extraction equipment Pending CN110981950A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911294437.XA CN110981950A (en) 2019-12-16 2019-12-16 Paddy-potato collagen extraction equipment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911294437.XA CN110981950A (en) 2019-12-16 2019-12-16 Paddy-potato collagen extraction equipment

Publications (1)

Publication Number Publication Date
CN110981950A true CN110981950A (en) 2020-04-10

Family

ID=70094274

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911294437.XA Pending CN110981950A (en) 2019-12-16 2019-12-16 Paddy-potato collagen extraction equipment

Country Status (1)

Country Link
CN (1) CN110981950A (en)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6375855B1 (en) * 1996-12-02 2002-04-23 Vivascience, Ag Method, device and apparatus for concentrating and/or purifying macromolecules in a solution
CN201670815U (en) * 2010-04-09 2010-12-15 新疆农业大学 Device for purifying albumen peptides
CN103539855A (en) * 2013-09-18 2014-01-29 温州科技职业学院 Extraction method of acaudina molpadioidea collagen
WO2016095685A1 (en) * 2014-12-20 2016-06-23 应跃斌 Pneumatic constant pressure continuous liquid feeding device and method
CN106497781A (en) * 2016-10-24 2017-03-15 世茂(苏州)生物科技有限公司 A kind of collagen protein extracting method of Fish collagen protein without fishy smell extraction equipment and based on which

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6375855B1 (en) * 1996-12-02 2002-04-23 Vivascience, Ag Method, device and apparatus for concentrating and/or purifying macromolecules in a solution
CN201670815U (en) * 2010-04-09 2010-12-15 新疆农业大学 Device for purifying albumen peptides
CN103539855A (en) * 2013-09-18 2014-01-29 温州科技职业学院 Extraction method of acaudina molpadioidea collagen
WO2016095685A1 (en) * 2014-12-20 2016-06-23 应跃斌 Pneumatic constant pressure continuous liquid feeding device and method
CN106497781A (en) * 2016-10-24 2017-03-15 世茂(苏州)生物科技有限公司 A kind of collagen protein extracting method of Fish collagen protein without fishy smell extraction equipment and based on which

Similar Documents

Publication Publication Date Title
CN204637760U (en) A kind of slipstream filter column combination purification devices of plural serial stage
CN102500005A (en) Blood transfusion system for autologous blood recovery, filtration and purification
CN216023494U (en) Vacuum filtering device for acid leaching liquid
CN110981950A (en) Paddy-potato collagen extraction equipment
CN208130597U (en) Cover the pressure filter of fructus lycii tablet raw material extracting solution
CN201453726U (en) Autogenous bone marrow blood refluxing device
CN212166749U (en) Filter machine
CN212403713U (en) Release agent recycling equipment
CN211672278U (en) Purification device is used in production of collagen peptide powder
CN214299447U (en) Environment-friendly coal industry public system water treatment facilities
CN201791429U (en) Fermented liquid alkali reclaiming device
CN206103401U (en) Vegetable protein draws concentrator
CN114177681A (en) Vacuum filtration type tool adsorption system
CN211394465U (en) Mesenchymal stem cell filtering separator
CN215756777U (en) Environment-friendly methallyl alcohol is waste liquid cauldron for preparation
CN214764578U (en) Reverse osmosis membrane medicine belt cleaning device
CN210287190U (en) Active collagen peptide liquid concentration and separation device
CN111705047A (en) Barley beta-amylase extraction method
CN217340451U (en) Traditional Chinese medicine pretreatment device for extracting traditional Chinese medicine polysaccharide
CN207567147U (en) A kind of separating-purifying device of animal collagen peptide
CN214385967U (en) Propolis extraction device
CN213295278U (en) Ultra-high pressure extraction equipment for processing acer truncatum oil
CN211310954U (en) Ultrafiltration type purified water purification device
CN218923938U (en) Separation and purification device for waste sulfuric acid containing organic matters
CN210085133U (en) Papermaking sewage treatment device

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20200410

RJ01 Rejection of invention patent application after publication