CN201670815U - Device for purifying albumen peptides - Google Patents
Device for purifying albumen peptides Download PDFInfo
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- CN201670815U CN201670815U CN2010201537783U CN201020153778U CN201670815U CN 201670815 U CN201670815 U CN 201670815U CN 2010201537783 U CN2010201537783 U CN 2010201537783U CN 201020153778 U CN201020153778 U CN 201020153778U CN 201670815 U CN201670815 U CN 201670815U
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Abstract
The utility model discloses a device for purifying albumen peptides. The device consists of a plate-frame ultrafilter, a power supply, a charging basket and a crystallizing tank which are connected with one other, wherein the plate-frame ultrafilter is connected with the power supply and is in circulating reflux communication with the charging basket; and the charging basket is communicated with the crystallizing tank through a pipeline. The device is characterized that: in the plate-frame ultrafilter, sealing plates on both sides of a flat-plate ultra-filtration membrane are replaced by passivated plate-like metal electrodes, an electrode lead passes through a membrane separator and is connected with two connector lugs fixed on both sides of a membrane component respectively and a distance between two electrodes can be adjusted to be between 0.07 and 1.0 centimeter by changing the form of a seal spacer. The device is widely applied in the field of purification of various albumen peptides.
Description
Technical field
The utility model relates to the separating and purifying technology field of biomolecules such as polysaccharide, protein, specifically is meant a kind of equipment technical field that protein peptide is purified that is used for.
Background technology
Biological big son generally is to separate to obtain from fermented liquid or enzyme reaction solution or animal and plant cells, compares with general chemical, and the separation and purification of biomolecules has following characteristics: the content of biomacromolecule is generally lower in the stock liquid; Stock liquid is multi-component mixture, and molecular weight distribution is extremely wide, and macromolecule materials such as nucleic acid, protein, polysaccharide, lipoid, phosphatide and lipopolysaccharides are arranged, and low molecular weight substances such as amino acid, organic bronsted lowry acids and bases bronsted lowry are also arranged; The less stable of biomacromolecule, chemical property own are stable inadequately, meet thermal lability, meet easily degraded of acid, make the separation and purification condition comparatively harsh; Specification of quality to the finished product is very high, and the biomacromolecule product generally is fine product such as medicine, biological reagent or food, must reach the requirement of pharmacopeia, reagent standard and codex Alimentarius.
At present, the unit that can select for use of the separation and purification of biomolecular material is quite limited.The biomolecules coarse-grain generally can pass through water extraction or rare salt, diluted alkaline extracts, and carries out elementary purifying through unit operations such as absorption, extraction, precipitation and centrifugations again, and wherein typical separation purification method is " water extract-alcohol precipitation " method.The device of separation and purification mainly contains centrifugal separating device, settler, ion exchange unit, affine adsorption unit, gel-filtration and ultra-filtration equipment etc.These devices differ from one another: centrifugal separating device can adapt to very wide scale scope, limits but its usefulness can be subjected to the effect of concentration of raw material and precipitation operation; Settler can be handled a large amount of materials equally, and it is subjected to the interfering substance effect littler than absorption or stratography, but will consume a large amount of reagent and long treatment time; Ion exchange unit is used for removing the compound that later separation is exerted an influence, and product purity is higher, but wants little several magnitude compared with centrifugal separating device on throughput; Affine adsorption unit is the most typical with affinity chromatography, its selectivity and specificity are extremely strong, are that other device is incomparable, have run into some because of restricted in development but fill the affinity chromatography column technology, compression and diffusion low as flow velocity, filler particles are transmitted slowly, thereby are difficult to large-scale application; Gel-filtration is usually used in the separation and the desalination of protein, polysaccharide aggregate, because the volume ratio of the glue filtration medium that rises is less, so the treatment capacity of process is little, the last of the purge process of generally being everlasting is used in the processing together; Ultrafiltration can normal-temperature operation, device is simple, energy consumption is low and selectivity is higher, has become the important means of separating mixture, but filtering rate descends and the film pollution is the subject matter that ultrafiltration runs in actual applications.At present, dialyse with continuous dialysis apparatus in the laboratory that abroad has, convenient and quick, but processing power is still less than normal, and the height of its efficient often depends on the concentration and the purity of crude product again.
Because biomolecular material such as polysaccharide and protein have multiple diversified function, have participated in the comings and goings of cell in biological phenomena, and are therefore, very active to the research of this class substance characteristics and function thereof.Along with going deep into that biomacromolecule is studied, begin to be paid attention to by industries such as medicine, biological reagent or food as functional polysaccharide and functional protein.As the key of the biomolecular material downstream course of processing, separation purifying technique faces the high requirement of super Song Yue: should satisfy the specification of product, reach bigger industrial scale again.
Summary of the invention
The utility model is exactly in order to solve above-mentioned the deficiencies in the prior art part, and a kind of equipment that protein peptide is purified that is used for is provided.This device reduces the number of steps in the biomacromolecule separating substances purifying flow process by the coupling of different separation and purification processes, and the optimization step combination, improves the rate of recovery, the reduction of product and produces investment and running cost; When improving quality product, improve the processing power of unit operation, realize scale effect; Improve the classification organic solvent precipitation method of traditional batch with the classification ultra-filtration membrane separating technology of serialization, reduce treatment time and reagent and consume, and make the molecular weight distribution of product more concentrated, more even.
A kind of equipment that protein peptide is purified that is used for described in the utility model, it is interconnected to constitute by plate and frame ultra-fine filter, power supply, charging basket and crystallizer, and wherein, the plate and frame ultra-fine filter is connected with power supply, and be communicated with the charging basket pump around circuit, charging basket is communicated with by pipeline with crystallizer.
In order to realize the utility model better, in described plate and frame ultra-fine filter, the tabular metal electrode replacement seal plate through Passivation Treatment can be used in the both sides of ultra-filtration membrane, and electrode cable passes the film device and is connected with two connector lugs that are fixed on the membrane module both sides respectively; Described two distance between electrodes can be regulated between 0.07～1.0cm by the form that changes gasket seal thickness.
The utility model compared with prior art has the following advantages and beneficial effect:
Electro ultrafiltration one dissolved is on the basis that electric field-enhanced ultrafiltration and concentration is handled, can dissolve each other with water but itself does not dissolve the organic solvent of biomacromolecule again by in good time adding is a certain amount of, play the water molecules " contention " around the solute molecule is come out, no longer solute molecule is played the constraint effect.Because the water molecules of being captured is still stayed in the solution, with the organic solvent that adds solution is played diluting effect, thereby reduced the viscosity of solution effectively again, help the operation of ultra-filtration and separation.This is just in time opposite with the situation that common degree of supersaturation raising must cause viscosity to rise, and therefore can increase the nucleation rate of solute greatly.Meanwhile, the electro ultrafiltration process leaches aqueous solvent with a comparatively stable speed, thereby the viscosity of solution is remained unchanged substantially when improving degree of supersaturation.In addition, because the membrane filtration process can make feed liquid be concentrated to a higher concentration before organic solvent adds, thus the input amount of saving solvent greatly.Bigger productive rate and output be can not only obtain, and the cost of separation and purification, the quality of raising final product reduced effectively.In addition, electro ultrafiltration one dilution crystallization method can obtain the crystal of target product at normal temperatures, need not to carry out traditional evaporation operation, is specially adapted to separation and the purifying of thermo-sensitivity biomacromolecule.Experimental result shows, adopts the processing dextran or the soybean peptides crude product of this device, product on purity near or reach the effect of gel filtration chromatography, and on the processing power than the big several magnitude of gel-filtration, meet or exceed traditional settler processing efficiency.This The Application of Technology can promote the development of biomaterial and living matter separating and purifying technology undoubtedly.
Description of drawings
Fig. 1 is that the utility model is used for the device structure synoptic diagram that protein peptide is purified.In Fig. 1:
1-charging basket, 2-crystallizer, 3-pump, 4-tensimeter, 5-plate and frame ultra-fine filter, 6-D.C. regulated power supply.
Embodiment
Below in conjunction with drawings and Examples, the utility model is done detailed description further.
Shown in accompanying drawing 1, plate and frame ultra-fine filter (5) is connected with D.C. regulated power supply (6), and is communicated with charging basket (1) pump around circuit by pump (3), and tensimeter (4) is installed on the connecting pipe, and charging basket (1) is communicated with by pipeline with crystallizer (2).Wherein, plate and frame ultra-fine filter (5) but be the plate and frame ultra-fine filter that an extra electric field is strengthened, on the basis that has the plate and frame ultra-fine filter of selling the market (useful area of film should be selected in the scope at 0.2～0.8M2) now, carry out structural modification, add plate-shape metal electrode through Passivation Treatment in the both sides of flat plate ultrafiltration membrane to replace the sealing plate on the conventional panels frame ultra-fine filter, the area of plate-shape metal electrode and form and sealing plate unanimity, two distance between electrodes can be regulated between 0.07～1.0cm by the form that changes gasket seal., after encapsulation process, pass the film device and be connected with two connector lugs that are fixed on the membrane module both sides respectively in order to the lead of giving two metal electrodes energisings.D.C. regulated power supply (6) directly adopts market D.C. regulated power supply on sale, require output voltage can be between 0V～50V step-less adjustment.Charging basket (1) is that a band stirs can thermoregulated charging basket, can be that volume is the metal buckets of 15L, strap clamp cover, and to regulate the temperature of charging basket, agitator is selected the electric mixer (rotating speed is 500-3000r/min, adjustable speed) of commercially available adjustable speed for use by recirculated water.Charging basket (1) is provided with the access port of organic solvent and stock liquid, and the relief outlet of concentrated solution.Crystallizer (2) is that a band stirs can thermoregulated crystallizer, can be that volume is the metal buckets of 2L, strap clamp cover, and to regulate the temperature of charging basket, agitator is selected the electric mixer (rotating speed is 500-3000r/min, adjustable speed) of commercially available adjustable speed for use by recirculated water.Crystallizer is provided with the access port of organic solvent and concentrated solution, and the relief outlet (crystalliferous dope can be delivered to centrifuge separator and carry out solid-liquid separation) of dope after the crystallization.
Electro ultrafiltration one dissolved can be regulated material temperature in charging basket and the crystallizer by the form of injecting recirculated water, and temperature can be controlled in 5 ℃-95 ℃ the scope, can be on the basis of the thermo-sensitivity of considering solute suitable rising feed temperature, to improve membrane flux; Pump can be selected constant flow pump or stage pump for use, and ultrafiltration pressure can be controlled at 0.1-0.5Mpa, adopts the mode of standard-sized sheet imported valve, adjusting outlet valve can obtain comparatively stable working pressure.For the uf processing of biomacromolecule, working pressure should be selected in the scope of 0.13～0.18Mpa, and membrane separating effect can be better.This device can be according to the process object corresponding ultra-filtration membrane that changes the outfit.The voltage range that the external dc voltage stabilized source can provide is 0～30V.
For the concentration that guarantees trapped fluid can increase quickly, adopt the total reflux form; The interpolation time of solvent and addition should be target to keep a membrane flux stable, less expensive according to ultra-filtration membrane variations of flux situation; For fear of at charging basket intercrystalline or growing the grain and cause pause in the production, can regularly the concentrated solution in the charging basket be entered crystallizer.
Realize that concrete workflow of the present utility model is:
1. close the imported valve of solvent and the outlet valve of concentrated solution, stock liquid is sent into charging basket, start constant flow pump and electro ultrafiltration device.
2. work as stock liquid and be concentrated to a certain degree (is foundation with " leaching liquid measure/stock liquid total amount "), open the imported valve of solvent and start the interior whipping appts of charging basket, disposable interpolation appropriate amount of organic is closed this valve then.
3. after the concentration of concentrated solution reaches supersaturation concentration, close the whipping appts in the charging basket and open the concentrated solution outlet valve, concentrated solution is sent into crystallizer, carry out crystallization by cooling or interpolation organic solvent, crystal separates with whizzer.
4. after the concentrated solution emptying in the charging basket, just can send into the separation and purification treatment that new stock liquid carries out a new round.So repeatedly.
5. the available 60 ℃ hot water injection 10min of the cleaning of ultra-filtration membrane gets final product.
6. in the separation and purification process of classification electro ultrafiltration one dilution crystallization, filtrate at the corresponding levels can be used as the stock liquid that next stage is handled.
Claims (2)
1. one kind is used for the equipment that protein peptide is purified, it is interconnected to constitute by plate and frame ultra-fine filter, power supply, charging basket and crystallizer, the plate and frame ultra-fine filter is connected with power supply, and be communicated with the charging basket pump around circuit, charging basket is communicated with by pipeline with crystallizer, it is characterized in that, in the plate and frame ultra-fine filter, the plate-shape metal electrode replacement seal plate of the both sides of flat plate ultrafiltration membrane through Passivation Treatment, electrode cable pass the film device and are connected with two connector lugs that are fixed on the membrane module both sides respectively.
2. a kind of equipment that protein peptide is purified that is used for according to claim 1 is characterized in that described two distance between electrodes can be regulated by the form that changes gasket seal between 0.07-1.0cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201537783U CN201670815U (en) | 2010-04-09 | 2010-04-09 | Device for purifying albumen peptides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010201537783U CN201670815U (en) | 2010-04-09 | 2010-04-09 | Device for purifying albumen peptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201670815U true CN201670815U (en) | 2010-12-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2010201537783U Expired - Fee Related CN201670815U (en) | 2010-04-09 | 2010-04-09 | Device for purifying albumen peptides |
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| Country | Link |
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| CN (1) | CN201670815U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110981950A (en) * | 2019-12-16 | 2020-04-10 | 温州科技职业学院 | Paddy-potato collagen extraction equipment |
-
2010
- 2010-04-09 CN CN2010201537783U patent/CN201670815U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110981950A (en) * | 2019-12-16 | 2020-04-10 | 温州科技职业学院 | Paddy-potato collagen extraction equipment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20101215 Termination date: 20130409 |