CN111705047A

CN111705047A - Barley beta-amylase extraction method

Info

Publication number: CN111705047A
Application number: CN202010683157.4A
Authority: CN
Inventors: 殷玲; 孔瑞扬; 华雅晴
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-07-15
Filing date: 2020-07-15
Publication date: 2020-09-25

Abstract

The invention relates to the field of amylase extraction, in particular to a barley beta-amylase extraction method, which comprises a base, a filtering mechanism, a storage mechanism, a conveying mechanism, a sealing mechanism, a fixing mechanism, a collecting mechanism and a locking universal wheel, wherein the filtering mechanism is fixedly connected to the upper end of the base, the filtering mechanism comprises a filtering box, a box cover, a mounting plate, stop blocks, filtering holes, a blocking net, diatomite and a filtering plate, the filtering box is fixedly connected to the upper end of the base, the box cover is connected to the upper end of the filtering box, the mounting plate is connected to the filtering box in a sliding mode, the two stop blocks are symmetrically and fixedly connected to the inner wall of the filtering box, the mounting plate is arranged at the upper ends of the two stop blocks, a plurality of filtering holes are vertically arranged in the mounting plate, and the blocking net is fixedly connected to the filtering holes and is. The extraction device provided by the invention has the advantages of good filtering effect and convenience in cleaning and replacing the filtering device.

Description

Barley beta-amylase extraction method

Technical Field

The invention relates to the field of amylase extraction, in particular to a barley beta-amylase extraction method.

Background

Beta-amylase, also known as beta-amylase, is yellowish or white in color, in lyophilized powder form, is used in biochemical studies, clinically as a digestive enzyme, for anorexia, dyspepsia, gastric mucositis, and the like. Beta-starch exists in barley, due to the popularity of barley, beta-amylase is generally extracted from barley, and in the process of extracting the beta-amylase from barley, enzymolysis liquid needs to be filtered.

Most of existing filtering equipment filters enzymatic hydrolysate through diatomite, a filtering device is generally fixedly connected through bolts or other connecting parts, the filtering device is not convenient to clean, and meanwhile, the diatomite is easy to expand and scatter in the beta-starch extraction process. Therefore, there is a need to provide a new method for extracting barley beta-amylase to solve the above technical problems.

Disclosure of Invention

Aiming at the problems in the prior art, the invention provides a barley beta-amylase extraction method.

The technical scheme adopted by the invention for solving the technical problems is as follows: a barley beta-amylase extraction method comprises the following steps:

s1: screening and cleaning raw material barley, draining moisture attached to the barley during cleaning, then adding the barley and water into a refiner for grinding to prepare barley homogenate, wherein the weight of the water added into the refiner is 1.7-1.85 times of that of the barley, then adding the prepared barley homogenate into an enzymolysis tank, adding moderate temperature alpha-amylase, beta-glucanase and neutral protease into the tank, and carrying out enzymolysis and leaching for 2-3 hours to obtain an enzymolysis solution;

s2: adding the enzymolysis liquid obtained in the step S1 into an extraction device, starting the extraction device, filtering and extracting the enzymolysis liquid through the extraction device to obtain pure stock solution, then heating the stock solution to 30-40 ℃, keeping the temperature for 25-30min, removing thermosetting component impurities in the stock solution, and then removing foreign proteins in the stock solution through an isoelectric point precipitation method to obtain a barley beta-amylase solution with high purity;

s3: carrying out fine filtration treatment on the barley beta-amylase solution obtained in the step S2 to obtain a barley beta-amylase solution with high purity, then evaporating the solution at low temperature under reduced pressure to remove most of water in the enzyme solution to obtain an enzyme solution with high concentration and purity, and then sealing and storing the enzyme solution;

wherein the extraction device in the step S2 comprises a base, a filtering mechanism, a material storage mechanism, a conveying mechanism, a sealing mechanism, a fixing mechanism and a collecting mechanism, the filtering mechanism is fixedly connected with the upper end of the base and comprises a filtering box, a box cover, a mounting plate, a stop block, filtering holes, a stop net, diatomite and a filtering plate, the filter box is fixedly connected with the upper end of the base, the box cover is connected with the upper end of the filter box, the mounting plate is connected in the filter box in a sliding manner, the two stop blocks are symmetrically and fixedly connected on the inner wall of the filter box, the mounting plate is arranged at the upper ends of the two stop blocks, a plurality of filtering holes are vertically arranged in the mounting plate, the blocking net is fixedly connected to the bottom of the filtering hole, the diatomite is filled in the filtering hole, and the filtering plate is connected in the filtering box in a sliding mode and located above the mounting plate; the material storage mechanism is fixedly connected to the upper end of the base; the conveying mechanism is fixedly connected to the material storage mechanism; the sealing mechanism is fixedly connected to the side wall of the lower side of the box cover; the fixing mechanism is fixedly connected to the side walls of the filter box and the box cover; the collecting mechanism is communicated with the interior of the filter box.

Preferably, storage mechanism includes storage case, storage chamber and feed inlet, storage case fixed connection is in the upper end of base, just the storage chamber is seted up in the storage case, the feed inlet is seted up in the upper end of storage case and is communicated with the storage chamber.

Preferably, conveying mechanism includes the pump body, inhales material pipe and conveying pipeline, pump body fixed connection is in the upper end of storage case, the pump body is through inhaling material pipe and storage cavity intercommunication, the pump body still communicates through conveying pipeline and case lid inside.

Preferably, sealing mechanism includes annular slot and annular inserted block, annular inserted block fixed connection is on the downside lateral wall of case lid, the annular slot is seted up in the upside lateral wall of rose box, just annular inserted block inserts and locates in the annular slot.

Preferably, the fixing mechanism comprises a first connecting block, a second connecting block and a fastening bolt, the first connecting block and the second connecting block are respectively and fixedly connected to the side walls of the box cover and the filter box, and the first connecting block and the second connecting block are fixedly connected through the fastening bolt.

Preferably, the collecting mechanism comprises a collecting box and a communicating pipe, the collecting box is located at the upper end of the base and is communicated with the inside of the filter box through the communicating pipe, and the lower end of the base is fixedly connected with two groups of symmetrically arranged locking universal wheels.

Compared with the related art, the extraction device provided by the invention has the following beneficial effects:

(1) in the extraction device provided by the invention, the enzymatic hydrolysate firstly falls onto the filter plate for primary filtration to remove larger impurities in the raw materials, the feed liquid after primary filtration flows into the filter holes and is subjected to secondary filtration through diatomite, the filtered clear liquid flows into the collection mechanism for storage, the baffle net is arranged to limit the diatomite in the filter holes, the baffle block is arranged to limit the mounting plate, the mounting plate is placed on the baffle block, the filter plate is placed above the mounting plate and is convenient to take out for cleaning and replacing, and the diatomite in the filter holes on the mounting plate is also convenient to clean and replace.

(2) According to the extraction device provided by the invention, the annular insert block is inserted into the annular chute, so that the connection sealing performance between the box cover and the filter box is improved, and meanwhile, the enzymolysis liquid in the filter box is prevented from flowing out from a gap between the filter box and the box cover.

Drawings

FIG. 1 is a schematic structural diagram of a preferred embodiment of the barley beta-amylase extraction method provided by the present invention;

FIG. 2 is a schematic structural view of the mounting plate shown in FIG. 1;

FIG. 3 is a schematic structural view of the filter plate shown in FIG. 1;

FIG. 4 is an enlarged schematic view of FIG. 1 at A;

FIG. 5 is a schematic view of the internal structure of the filtration pores shown in FIG. 2;

FIG. 6 is a flow chart of a method of the present invention.

Reference numbers in the figures: 1. the base, 2, filter mechanism, 21, the rose box, 22, the case lid, 23, the mounting panel, 24, the dog, 25, the filtration pore, 26, keep off the net, 27, diatomaceous earth, 28, the filter, 3, storage mechanism, 31, the storage case, 32, the storage chamber, 33, the feed inlet, 4, conveying mechanism, 41, the pump body, 42, inhale the material pipe, 43, the conveying pipeline, 5, sealing mechanism, 51, annular slot, 52, annular inserted block, 6, fixed establishment, 61, first connecting block, 62, the second connecting block, 63, fastening bolt, 7, collection mechanism, 71, collecting box, 72, communicating pipe, 8, the locking universal wheel.

Detailed Description

The invention is further described with reference to the following figures and embodiments.

Referring to fig. 1-6, a method for extracting barley beta-amylase comprises the following steps:

wherein, in the step S2, the extraction device comprises a base 1, a filter mechanism 2, a storage mechanism 3, a conveying mechanism 4, a sealing mechanism 5, a fixing mechanism 6, a collecting mechanism 7 and a locking universal wheel 8, wherein the filter mechanism 2 is fixedly connected to the upper end of the base 1, the filter mechanism 2 comprises a filter box 21, a box cover 22, a mounting plate 23, a baffle 24, filter holes 25, a baffle net 26, diatomite 27 and a filter plate 28, the filter box 21 is fixedly connected to the upper end of the base 1, the box cover 22 is connected to the upper end of the filter box 21, the mounting plate 23 is slidably connected in the filter box 21, the two baffle blocks 24 are symmetrically and fixedly connected to the inner wall of the filter box 21, the mounting plate 23 is arranged at the upper ends of the two baffle blocks 24, the plurality of filter holes 25 are vertically arranged in the mounting plate 23, the baffle net 26 is fixedly connected to the bottoms of the filter holes 25, the diatomite 27 is filled in the filter, the device is beneficial to placing the diatomite 27 in the filter holes 25, the diatomite 27 is placed to be scattered in the process of extracting amylase, the filter plate 28 is connected in the filter box 21 in a sliding manner and is positioned above the mounting plate 23, in the using process of the device, the box cover 22 and the filter box 21 are connected and sealed through the sealing mechanism 5, the box cover 22 is fixed on the filter box 21 through the fixing mechanism 6, raw materials are stored in the storage mechanism 3 and are conveyed into the filter box 21 through the conveying mechanism 4, the enzymatic hydrolysate firstly falls onto the filter plate 28 for primary filtration, larger impurities in the raw materials are removed, the filtered material liquid flows into the filter holes 25 for secondary filtration through the diatomite 27, the filtered clear liquid flows into the collecting mechanism 7 for storage, the baffle net 26 is arranged to limit the diatomite 27 in the filter holes 25, the baffle block 24 is arranged to limit the mounting plate 23, mounting panel 23 is placed on dog 24, and filter 28 is placed in mounting panel 23 top, and filter 28 is convenient for take out and washs the change to it, also is convenient for clear up the change to the diatomaceous earth 27 in the filtration pore 25 on mounting panel 23, and this device easy operation, convenient to use through secondary filter, has improved the filter effect of enzymolysis liquid greatly, is convenient for wash the change to filter equipment (filter 28 and diatomaceous earth 27) simultaneously.

Referring to fig. 1 and 3, the storage mechanism 3 is fixedly connected to the upper end of the base 1, the storage mechanism 3 includes a storage box 31, a storage cavity 32 and a feeding hole 33, the storage box 31 is fixedly connected to the upper end of the base 1, the storage cavity 32 is opened in the storage box 31, the feeding hole 33 is opened in the upper end of the storage box 31 and is communicated with the storage cavity 32, the enzymatic hydrolysate can be poured into the storage cavity 32 from the feeding hole 33, and the enzymatic hydrolysate is stored in the storage cavity 32 in the storage box 31.

Referring to fig. 1, conveying mechanism 4 is fixedly connected to storage mechanism 3, conveying mechanism 4 includes the pump body 41, inhale material pipe 42 and conveying pipeline 43, pump body 41 is fixedly connected to the upper end of storage tank 31, pump body 41 communicates with storage cavity 32 through inhaling material pipe 42, pump body 41 still communicates with the inside of case lid 22 through conveying pipeline 43, in carrying out the transportation process to the enzymolysis liquid, pump body 41 absorbs the enzymolysis liquid in storage cavity 32 through inhaling material pipe 42, the rethread conveying pipeline 43 sends the enzymolysis liquid to filter box 21 and filters.

Referring to fig. 1, the sealing mechanism 5 is fixedly connected to the lower side wall of the box cover 22, the sealing mechanism 5 includes an annular insertion groove 51 and an annular insertion block 52, the annular insertion block 52 is fixedly connected to the lower side wall of the box cover 22, the annular insertion groove 51 is opened in the upper side wall of the filter box 21, the annular insertion block 52 is inserted in the annular insertion groove 51, and the annular insertion block 52 is inserted in the annular sliding groove 51, so that the connection sealing performance between the box cover 22 and the filter box 21 is improved, and the enzymatic hydrolysate in the filter box 21 is prevented from flowing out from the gap between the filter box 21 and the box cover 22.

Referring to fig. 1, the fixing mechanism 6 is fixedly connected to the side walls of the filter box 21 and the box cover 22, the fixing mechanism 6 includes a first connecting block 61, a second connecting block 62 and a fastening bolt 63, the first connecting block 61 and the second connecting block 62 are respectively fixedly connected to the side walls of the box cover 22 and the filter box 21, the first connecting block 61 and the second connecting block 62 are fixedly connected through the fastening bolt 63, and the first connecting block 61 and the second connecting block 62 are fixedly connected through the fastening bolt 63, so that the box cover 22 and the filter box 21 are fixedly connected.

Referring to fig. 1, the collecting mechanism 7 is communicated with the inside of the filter box 21, the collecting mechanism 7 comprises a collecting box 71 and a communicating pipe 72, the collecting box 71 is located at the upper end of the base 1, the collecting box 71 is communicated with the inside of the filter box 21 through the communicating pipe 72, the filtered clear liquid in the filter box 21 flows into the collecting box 71 through the communicating pipe 72 to be stored in a centralized manner, the next step of processing is facilitated, two sets of locking universal wheels 8 are symmetrically arranged and fixedly connected to the lower end of the base 1, and the locking universal wheels 8 are arranged at the lower end of the base 1, so that the device can be moved and braked.

The working principle of the device is as follows: in the using process of the extraction device, the feed inlet 33 can fill the storage cavity 32 with the enzymatic hydrolysate, the enzymatic hydrolysate is stored in the storage cavity 32 in the storage box 31, the pump body 41 sucks the enzymatic hydrolysate in the storage cavity 32 through the suction pipe 42 in the process of conveying the enzymatic hydrolysate, the enzymatic hydrolysate is conveyed into the filter box 21 through the conveying pipe 43 for filtering, the enzymatic hydrolysate falls onto the filter plate 28 for primary filtering to remove larger impurities in the raw materials, the feed liquid after the primary filtering flows into the filter hole 25 and is subjected to secondary filtering through the diatomite 27, the clear liquid after the filtering in the filter box 21 flows into the collection box 71 through the communicating pipe 72 for centralized storage, the processing of the next working procedure is convenient, the baffle net 26 is arranged to limit the diatomite 27 in the filter hole 25, the baffle block 24 is arranged to limit the mounting plate 23, the mounting panel 23 is placed on dog 24, the filter 28 is placed in mounting panel 23 top, when case lid 22 is opened to needs and filter 28 and diatomaceous earth 27 wash the change, it is fixed to remove the restriction of fastening bolt 63 to first connecting block 61 and second connecting block 62, can take off case lid 22 from filter box 21 top, take out filter 28 and mounting panel 23, the filter 28 is convenient for take out and washs it and change, also clean up the diatomaceous earth 27 in the filtration pore 25 on the mounting panel 23 and change.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the embodiments and descriptions given above are only illustrative of the principles of the present invention, and various changes and modifications may be made without departing from the spirit and scope of the invention, which fall within the scope of the claims. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The barley beta-amylase extraction method is characterized by comprising the following steps:

wherein, in the step S2, the extraction device comprises a base (1), a filtering mechanism (2), a storage mechanism (3), a conveying mechanism (4), a sealing mechanism (5), a fixing mechanism (6) and a collecting mechanism (7), the filtering mechanism (2) is fixedly connected with the upper end of the base (1), the filtering mechanism (2) comprises a filtering box (21), a box cover (22), a mounting plate (23), a stop block (24), a filtering hole (25), a baffle net (26), diatomite (27) and a filtering plate (28), the filtering box (21) is fixedly connected with the upper end of the base (1), the box cover (22) is connected with the upper end of the filtering box (21), the mounting plate (23) is slidably connected in the filtering box (21), the two stop blocks (24) are symmetrically and fixedly connected on the inner wall of the filtering box (21), and the mounting plate (23) is arranged at the upper ends of the two stop blocks (24), the filter plates (28) are connected in the filter box (21) in a sliding manner and positioned above the mounting plate (23); the material storage mechanism (3) is fixedly connected to the upper end of the base (1); the conveying mechanism (4) is fixedly connected to the material storage mechanism (3); the sealing mechanism (5) is fixedly connected to the side wall of the lower side of the box cover (22); the fixing mechanism (6) is fixedly connected to the side walls of the filter box (21) and the box cover (22); the collecting mechanism (7) is communicated with the interior of the filter box (21).

2. The barley beta-amylase extraction method according to claim 1, wherein the storage mechanism (3) comprises a storage box (31), a storage cavity (32) and a feed port (33), the storage box (31) is fixedly connected to the upper end of the base (1), the storage cavity (32) is arranged in the storage box (31), and the feed port (33) is arranged at the upper end of the storage box (31) and is communicated with the storage cavity (32).

3. The barley beta-amylase extraction method according to claim 2, wherein the conveying mechanism (4) comprises a pump body (41), a suction pipe (42) and a conveying pipe (43), the pump body (41) is fixedly connected to the upper end of the storage tank (31), the pump body (41) is communicated with the storage cavity (32) through the suction pipe (42), and the pump body (41) is further communicated with the interior of the tank cover (22) through the conveying pipe (43).

4. The barley beta-amylase extraction method according to claim 1, wherein the sealing mechanism (5) comprises an annular slot (51) and an annular insert block (52), the annular insert block (52) is fixedly connected to the lower side wall of the box cover (22), the annular slot (51) is opened in the upper side wall of the filter box (21), and the annular insert block (52) is inserted in the annular slot (51).

5. The barley beta-amylase extraction method according to claim 1, wherein the fixing mechanism (6) comprises a first connecting block (61), a second connecting block (62) and a fastening bolt (63), the first connecting block (61) and the second connecting block (62) are respectively fixedly connected to the side walls of the box cover (22) and the filter box (21), and the first connecting block (61) and the second connecting block (62) are fixedly connected through the fastening bolt (63).

6. The barley beta-amylase extraction method according to claim 1, wherein the collection mechanism (7) comprises a collection tank (71) and a communication pipe (72), the collection tank (71) is positioned at the upper end of the base (1), and the collection tank (71) is communicated with the inside of the filter tank (21) through the communication pipe (72); the lower end of the base (1) is fixedly connected with two groups of locking universal wheels (8) which are symmetrically arranged.