CN110974755A - Natural anti-aging mask and preparation method and application thereof - Google Patents
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- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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Abstract
The invention provides a natural anti-aging mask and a preparation method and application thereof, belonging to the technical field of cosmetic preparation. The natural anti-aging mask comprises mask paper and mask liquid, wherein the mask liquid comprises the following components in parts by weight: 15-25 parts of sargassum thunbergii polypeptide, 0.5-2 parts of trehalose, 0.5-2 parts of sodium hyaluronate, 1-3 parts of clove extract, 1-3 parts of grape extract, 1-3 parts of green tea extract, 0.5-2 parts of pawpaw extract, 0.2-0.5 part of rose essential oil and 200 parts of sterile deionized water. The Sargassum thunbergii polypeptide added in the facial mask liquid can obviously eliminate hydroxyl free radicals and DPPH free radicals, so that the effect of inhibiting skin aging is achieved.
Description
Technical Field
The invention belongs to the technical field of cosmetic preparation, and particularly relates to a natural anti-aging mask and a preparation method and application thereof.
Background
Skin aging is a result of oxidation, and a large number of free radicals are generated during aerobic respiration of skin cells, which are major factors causing skin aging. According to research, the skin aging is caused by 80% of free radicals, on one hand, oxygen radicals destroy skin cells, so that microcirculation of the skin cells is disturbed, basal cells of the skin cannot obtain nutrition, and the cells are shriveled; on the other hand, excessive oxygen radical deposition can lead to metabolic disorders in skin cells, melanin deposition, and skin darkness and color spots.
During the research process of protein, researchers find a substance which is composed of amino acid and is different from protein, and the substance has double effects of regulating physiological functions of organisms and providing nutrition for the organisms and is called polypeptide. Compared with protein, the polypeptide has good processing performance, higher bioavailability and easier absorption by human body than amino acid. The bioactive peptide has the important functions of resisting fatigue, lowering blood pressure, resisting oxidation, regulating immunity, resisting tumor, resisting bacteria, lowering blood sugar, etc. Meanwhile, the research finds that the polypeptide can relieve the oxidative damage of the organism by eliminating excessive free radicals. Therefore, it is of great significance to find new naturally extracted polypeptides for inhibiting skin aging.
Disclosure of Invention
The invention aims to provide a natural anti-aging mask with good anti-aging effect and no toxic or side effect.
In order to achieve the aim, the invention provides a natural anti-aging mask, which comprises mask paper and mask liquid, wherein the mask liquid comprises the following components: sargassum thunbergii polypeptide, trehalose, sodium hyaluronate, clove extract, grape extract, green tea extract, papaya extract, rose essential oil and sterile deionized water.
Preferably, the facial mask liquid comprises 15-25 parts of sargassum thunbergii polypeptide, 0.5-2 parts of trehalose, 0.5-2 parts of sodium hyaluronate, 1-3 parts of clove extract, 1-3 parts of grape extract, 1-3 parts of green tea extract, 0.5-2 parts of pawpaw extract, 0.2-0.5 part of rose essential oil and 200 parts of sterile deionized water.
Preferably, the extraction method of the sargassum thunbergii polypeptide comprises the following steps:
(1) grinding dried Sargassum thunbergii into powder with liquid nitrogen;
(2) adding 10-15 times volume of water, ultrasonic extracting for 1-2 hr, filtering, centrifuging at 3000r/min for 10-20min, and removing precipitate to obtain crude extract of Sargassum thunbergii;
(3) adding 0.05-0.1 times of cellulase into the crude extract of Sargassum thunbergii, performing enzymolysis at 50-60 deg.C and pH of 4.5-5.5 for 2-3 hr;
(4) maintaining the temperature, adjusting pH to 6-6.5, adding papain 0.05-0.1 times, and performing enzymolysis for 3-4 hr;
(5) reducing the temperature to 35-40 deg.C, adjusting pH to 7-8, adding 0.05-0.2 times of trypsin, and performing enzymolysis for 3-4 hr;
(6) heating to 90-95 deg.C for 30min, and inactivating enzyme;
(7) cooling to room temperature, centrifuging at 3000r/min for 30-40min to obtain crude extract of Sargassum thunbergii polypeptide;
(8) filtering the sargassum thunbergii polypeptide by using a 500-2000Da nanofiltration membrane to obtain a sargassum thunbergii polypeptide refined extraction solution;
(9) freeze-drying at low temperature to obtain Sargassum thunbergii polypeptide.
Preferably, the clove extract, the grape extract, the green tea extract and the papaya extract are extracted by 60% ethanol water solution.
In addition, the invention provides a preparation method of the anti-aging mask, which comprises the following steps:
(1) weighing the raw materials in parts by mass;
(2) heating sterile deionized water to 50-65 deg.C, adding trehalose, sodium hyaluronate, flos Caryophylli extract, fructus Vitis Viniferae extract, green tea extract, and fructus Chaenomelis extract, stirring to dissolve completely;
(3) cooling to 35-45 deg.C, adding Sargassum thunbergii polypeptide, stirring to dissolve completely;
(4) cooling to 25-30 deg.C, adding flos Rosae Rugosae essential oil, and making into facial mask liquid;
(5) sterile filtration is carried out by using a filter membrane liquid with the diameter of 0.22 mu m;
(6) and (3) placing the facial mask paper into a facial mask bag, and adding sterile facial mask liquid to obtain the natural anti-aging facial mask.
In addition, the invention provides application of the natural whitening mask, and the mask is directly attached to the skin to reduce the secretion of free radicals of skin cells, so that the effect of delaying aging is achieved.
The invention has the beneficial effects that
(1) The sargassum thunbergii polypeptide added in the facial mask liquid can obviously eliminate hydroxyl free radicals and DPPH free radicals, so that the effect of inhibiting skin aging is achieved.
(2) The natural anti-aging mask provided by the invention selects plant extracts, does not contain preservatives and has small irritation to skin.
Detailed Description
Example 1
1. Preparation of Sargassum thunbergii polypeptide
(1) Grinding dried Sargassum thunbergii into powder with liquid nitrogen;
(2) adding 10 times volume of water, ultrasonically extracting for 1 hr, filtering, centrifuging at 3000r/min for 10min, and removing precipitate to obtain crude extract of Sargassum thunbergii;
(3) adding 0.05 times of cellulase into the crude extract of Sargassum thunbergii, performing enzymolysis at 50 deg.C and pH of 4.5 for 2 hr;
(4) maintaining the temperature, adjusting the pH value to 6, adding 0.05 times of papain, and performing enzymolysis for 3 hours;
(5) reducing the temperature to 35 ℃, adjusting the pH value to 7, adding 0.05 times of trypsin, and performing enzymolysis for 3 hours;
(6) heating to 90 deg.C for 30min, and inactivating enzyme;
(7) cooling to room temperature, centrifuging at 3000r/min for 30min to obtain crude extract of Sargassum thunbergii polypeptide;
(8) filtering the sargassum thunbergii polypeptide by using a 500-2000Da nanofiltration membrane to obtain a sargassum thunbergii polypeptide refined extraction solution;
(9) freeze-drying at low temperature to obtain Sargassum thunbergii polypeptide.
2. Preparation of anti-aging mask
(1) Weighing 15 parts of sargassum thunbergii polypeptide, 2 parts of trehalose, 2 parts of sodium hyaluronate, 3 parts of clove extract, 3 parts of grape extract, 3 parts of green tea extract, 2 parts of papaya extract, 0.5 part of rose essential oil and 150 parts of sterile deionized water according to mass parts;
(2) heating sterile deionized water to 50 deg.C, adding trehalose, sodium hyaluronate, flos Caryophylli extract, fructus Vitis Viniferae extract, green tea extract, and fructus Chaenomelis extract, stirring to dissolve completely;
(3) cooling to 35 deg.C, adding Sargassum thunbergii polypeptide, stirring to dissolve completely;
(4) cooling to 25 deg.C, adding flos Rosae Rugosae essential oil to obtain facial mask liquid;
(5) sterile filtration is carried out by using a filter membrane liquid with the diameter of 0.22 mu m;
(6) and (3) placing the facial mask paper into a facial mask bag, and adding sterile facial mask liquid to obtain the natural anti-aging facial mask 1.
Example 2
1. Preparation of Sargassum thunbergii polypeptide
(1) Grinding dried Sargassum thunbergii into powder with liquid nitrogen;
(2) adding 15 times volume of water, ultrasonically extracting for 2 hr, filtering, centrifuging at 3000r/min for 20min, and removing precipitate to obtain crude extract of Sargassum thunbergii;
(3) adding 0.1 time of cellulase into the crude extract of the sargassum thunbergii, carrying out enzymolysis for 3 hours at the temperature of 60 ℃ and the pH value of 5.5;
(4) maintaining the temperature, adjusting the pH value to 6.5, adding 0.1 time of papain, and performing enzymolysis for 4 hours;
(5) reducing the temperature to 40 ℃, adjusting the pH value to 8, adding 0.2 times of trypsin, and performing enzymolysis for 3-4 hours;
(6) heating to 95 deg.C for 30min, and inactivating enzyme;
(7) cooling to room temperature, centrifuging at 3000r/min for 40min to obtain crude extract of Sargassum thunbergii polypeptide;
(8) filtering the sargassum thunbergii polypeptide by using a 500-2000Da nanofiltration membrane to obtain a sargassum thunbergii polypeptide refined extraction solution;
(9) freeze-drying at low temperature to obtain Sargassum thunbergii polypeptide.
2. Preparation of anti-aging mask
(1) Weighing 15 parts of sargassum thunbergii polypeptide, 2 parts of trehalose, 2 parts of sodium hyaluronate, 3 parts of clove extract, 3 parts of grape extract, 3 parts of green tea extract, 2 parts of papaya extract, 0.5 part of rose essential oil and 150 parts of sterile deionized water according to mass parts;
(2) heating sterile deionized water to 50 deg.C, adding trehalose, sodium hyaluronate, flos Caryophylli extract, fructus Vitis Viniferae extract, green tea extract, and fructus Chaenomelis extract, stirring to dissolve completely;
(3) cooling to 35 deg.C, adding Sargassum thunbergii polypeptide, stirring to dissolve completely;
(4) cooling to 25 deg.C, adding flos Rosae Rugosae essential oil to obtain facial mask liquid;
(5) sterile filtration is carried out by using a filter membrane liquid with the diameter of 0.22 mu m;
(6) and (3) placing the facial mask paper into a facial mask bag, and adding sterile facial mask liquid to obtain the natural anti-aging facial mask 2.
Example 3
1. Preparation of Sargassum thunbergii polypeptide
(1) Grinding dried Sargassum thunbergii into powder with liquid nitrogen;
(2) adding 10 times volume of water, ultrasonically extracting for 1 hr, filtering, centrifuging at 3000r/min for 10min, and removing precipitate to obtain crude extract of Sargassum thunbergii;
(3) adding 0.05 times of cellulase into the crude extract of Sargassum thunbergii, performing enzymolysis at 50 deg.C and pH of 4.5 for 2-3 hr;
(4) maintaining the temperature, adjusting the pH value to 6, adding 0.05 times of papain, and performing enzymolysis for 3 hours;
(5) reducing the temperature to 35 ℃, adjusting the pH value to 7, adding 0.05 times of trypsin, and performing enzymolysis for 3 hours;
(6) heating to 90 deg.C for 30min, and inactivating enzyme;
(7) cooling to room temperature, centrifuging at 3000r/min for 30min to obtain crude extract of Sargassum thunbergii polypeptide;
(8) filtering the sargassum thunbergii polypeptide by using a 500-2000Da nanofiltration membrane to obtain a sargassum thunbergii polypeptide refined extraction solution;
(9) freeze-drying at low temperature to obtain Sargassum thunbergii polypeptide.
2. Preparation of anti-aging mask
(1) Weighing 25 parts of sargassum thunbergii polypeptide, 0.5 part of trehalose, 0.5 part of sodium hyaluronate, 1 part of clove extract, 1 part of grape extract, 1 part of green tea extract, 0.5 part of pawpaw extract, 0.2 part of rose essential oil and 200 parts of sterile deionized water according to mass parts.
(2) Heating sterile deionized water to 65 deg.C, adding trehalose, sodium hyaluronate, flos Caryophylli extract, fructus Vitis Viniferae extract, green tea extract, and fructus Chaenomelis extract, stirring to dissolve completely;
(3) cooling to 45 deg.C, adding Sargassum thunbergii polypeptide, stirring to dissolve completely;
(4) cooling to 30 deg.C, adding flos Rosae Rugosae essential oil, and making into facial mask liquid;
(5) sterile filtration is carried out by using a filter membrane liquid with the diameter of 0.22 mu m;
(6) and (3) placing the facial mask paper into a facial mask bag, and adding sterile facial mask liquid to obtain the natural anti-aging facial mask 3.
Example 4
1. Preparation of Sargassum thunbergii polypeptide
(1) Grinding dried Sargassum thunbergii into powder with liquid nitrogen;
(2) adding 15 times volume of water, ultrasonically extracting for 2 hr, filtering, centrifuging at 3000r/min for 20min, and removing precipitate to obtain crude extract of Sargassum thunbergii;
(3) adding 0.1 time of cellulase into the crude extract of the sargassum thunbergii, carrying out enzymolysis for 3 hours at the temperature of 60 ℃ and the pH value of 5.5;
(4) maintaining the temperature, adjusting the pH value to 6.5, adding 0.1 time of papain, and performing enzymolysis for 4 hours;
(5) reducing the temperature to 40 ℃, adjusting the pH value to 8, adding 0.2 times of trypsin, and performing enzymolysis for 3-4 hours;
(6) heating to 95 deg.C for 30min, and inactivating enzyme;
(7) cooling to room temperature, centrifuging at 3000r/min for 40min to obtain crude extract of Sargassum thunbergii polypeptide;
(8) filtering the sargassum thunbergii polypeptide by using a 500-2000Da nanofiltration membrane to obtain a sargassum thunbergii polypeptide refined extraction solution;
(9) freeze-drying at low temperature to obtain Sargassum thunbergii polypeptide.
2. Preparation of anti-aging mask
(1) Weighing 25 parts of sargassum thunbergii polypeptide, 0.5 part of trehalose, 0.5 part of sodium hyaluronate, 1 part of clove extract, 1 part of grape extract, 1 part of green tea extract, 0.5 part of pawpaw extract, 0.2 part of rose essential oil and 200 parts of sterile deionized water according to mass parts.
(2) Heating sterile deionized water to 65 deg.C, adding trehalose, sodium hyaluronate, flos Caryophylli extract, fructus Vitis Viniferae extract, green tea extract, and fructus Chaenomelis extract, stirring to dissolve completely;
(3) cooling to 45 deg.C, adding Sargassum thunbergii polypeptide, stirring to dissolve completely;
(4) cooling to 30 deg.C, adding flos Rosae Rugosae essential oil, and making into facial mask liquid;
(5) sterile filtration is carried out by using a filter membrane liquid with the diameter of 0.22 mu m;
(6) and (3) placing the facial mask paper into a facial mask bag, and adding sterile facial mask liquid to obtain the natural anti-aging facial mask 4.
Example 5
Determination of hydroxyl radical scavenging ability of sargassum thunbergii polypeptide
Taking 1.0 mL of anhydrous ethanol solution of 1.865 mmol/L o-diazaphenanthrene in a test tube with a plug, respectively adding 2mL of 2M PH7.4 phosphate buffer solution and 1mL of 2.5%, 5%, 7.5% and 10% sargassum thunbergii polypeptide, fully and uniformly mixing, adding 1.0mL of 1.865mmol/L FeSO47H2O solution, mixed again and then 1.0mL of 0.03% (v/v) H2O2The absorbance of each mixed solution was measured at 536nm after 60min in a constant temperature water bath at 37 ℃.
The formula for the calculation of the hydroxyl radical is:
hydroxyl radical clearance (%) = [ (a)s-An)/(Ab-An)] × 100。
ASIs the absorbance of Sargassum thunbergii polypeptide, Ab is the absorbance value measured by using distilled water instead of Sargassum thunbergii polypeptide as blank group, and An is the absorbance value measured by using distilled water instead of H2O2The absorbance obtained for the lesion group was used.
As can be seen from fig. 1, the sargassum thunbergii polypeptides have better hydroxyl radical clearance, and 10% of sargassum thunbergii polypeptides have 74.71% hydroxyl radical clearance.
Example 6
Determination of DPPH radical scavenging Rate
Taking 2.0mL DPPH solution (solvent is absolute ethanol), adding 2.5%, 5%, 7.5%, 10% sargassum thunbergii polypeptide (each group is set to 3 times), mixing, reacting at 25 deg.C in the dark for 1h, measuring absorbance at 517nm as AS(ii) a The control group is 2.0mL of ethanol solution and 2.0mL of sample, and the absorbance is Ab(ii) a Blank set was 2.0mLl ethanol solution and 2.0mL DPPH solution with absorbance A0.
DPPH free radical clearance calculation formula:
DPPH clearance = (1- (a)S-Ab）/A0）× 100
As can be seen from fig. 2, the sargassum thunbergii polypeptides have better DPPH free radical clearance, and 10% sargassum thunbergii polypeptides have DPPH free radical clearance of 83.28%.
In conclusion, the sargassum thunbergii polypeptide has better definition rate on hydroxyl free radicals and DPPH free radicals, and the sargassum thunbergii polypeptide can play a role in delaying senescence.
Claims (6)
1. The natural anti-aging mask is characterized by comprising mask paper and mask liquid, wherein the mask liquid comprises the following components: sargassum thunbergii polypeptide, trehalose, sodium hyaluronate, clove extract, grape extract, green tea extract, papaya extract, rose essential oil and sterile deionized water.
2. The natural anti-aging mask as claimed in claim 1, wherein the mask solution comprises 15-25 parts of sargassum thunbergii polypeptide, 0.5-2 parts of trehalose, 0.5-2 parts of sodium hyaluronate, 1-3 parts of clove extract, 1-3 parts of grape extract, 1-3 parts of green tea extract, 0.5-2 parts of papaya extract, 0.2-0.5 part of rose essential oil and 150 parts of sterile deionized water.
3. The natural anti-aging mask as claimed in claim 1 or 2, wherein the extraction method of sargassum thunbergii polypeptides is as follows:
(1) grinding dried Sargassum thunbergii into powder with liquid nitrogen;
(2) adding 10-15 times volume of water, ultrasonic extracting for 1-2 hr, filtering, centrifuging at 3000r/min for 10-20min, and removing precipitate to obtain crude extract of Sargassum thunbergii;
(3) adding 0.05-0.1 times of cellulase into the crude extract of Sargassum thunbergii, performing enzymolysis at 50-60 deg.C and pH of 4.5-5.5 for 2-3 hr;
(4) maintaining the temperature, adjusting pH to 6-6.5, adding papain 0.05-0.1 times, and performing enzymolysis for 3-4 hr;
(5) reducing the temperature to 35-40 deg.C, adjusting pH to 7-8, adding 0.05-0.2 times of trypsin, and performing enzymolysis for 3-4 hr;
(6) heating to 90-95 deg.C for 30min, and inactivating enzyme;
(7) cooling to room temperature, centrifuging at 3000r/min for 30-40min to obtain crude extract of Sargassum thunbergii polypeptide;
(8) filtering the sargassum thunbergii polypeptide by using a 500-2000Da nanofiltration membrane to obtain a sargassum thunbergii polypeptide refined extraction solution;
(9) freeze-drying at low temperature to obtain Sargassum thunbergii polypeptide.
4. The natural facial mask for anti-aging according to claim 1 or 2, wherein the clove extract, the grape extract, the green tea extract, and the papaya extract are extracted from 60% ethanol aqueous solution.
5. The preparation method of the anti-aging mask is characterized by comprising the following steps:
(1) weighing the raw materials in parts by mass;
(2) heating sterile deionized water to 50-65 deg.C, adding trehalose, sodium hyaluronate, flos Caryophylli extract, fructus Vitis Viniferae extract, green tea extract, and fructus Chaenomelis extract, stirring to dissolve completely;
(3) cooling to 35-45 deg.C, adding Sargassum thunbergii polypeptide, stirring to dissolve completely;
(4) cooling to 25-30 deg.C, adding flos Rosae Rugosae essential oil, and making into facial mask liquid;
(5) sterile filtration is carried out by using a filter membrane liquid with the diameter of 0.22 mu m;
(6) and (3) placing the facial mask paper into a facial mask bag, and adding sterile facial mask liquid to obtain the natural anti-aging facial mask.
6. The application of the natural whitening mask is characterized in that the mask is directly attached to the skin to reduce the secretion of free radicals of skin cells, so that the effect of delaying aging is achieved.
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