CN110972897A - Preparation method and application of special microorganism seedling culture substrate for rice - Google Patents
Preparation method and application of special microorganism seedling culture substrate for rice Download PDFInfo
- Publication number
- CN110972897A CN110972897A CN201911391465.3A CN201911391465A CN110972897A CN 110972897 A CN110972897 A CN 110972897A CN 201911391465 A CN201911391465 A CN 201911391465A CN 110972897 A CN110972897 A CN 110972897A
- Authority
- CN
- China
- Prior art keywords
- rice
- seedling
- substrate
- culture substrate
- microbial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
Abstract
The invention discloses a preparation method and application of a special microbial seedling culture substrate for rice. The main components of the composition are as follows: peat soil, vermiculite, biochar, decomposed chicken manure, river sand, biocontrol bacteria and the like. The seedling culture substrate has the particle size of 1-10mm, the pH value of 6.8-7.3, the conductivity of 3.0-3.4ms/cm and the spore content of 107The prevention effect on damping off and bakanae disease of rice at the seedling stage reaches more than 65 percent per gram. The microbial seedling culture substrate can provide a rice seedling stage growing stationAll needed nutrients are not needed to be irrigated with nutrient solution, and simultaneously, the occurrence of seedling blight and bakanae disease of rice in the seedling stage can be effectively prevented and treated. The seedling raising substrate is applied to raising seedlings of rice.
Description
Technical Field
The invention belongs to the technical field of agricultural production, and particularly relates to a microbial seedling raising matrix which has the advantages of nutrition, air permeability, water retention and disease prevention and is suitable for raising rice seedlings.
Background
High-quality rice seedlings are the precondition of high yield of rice, a substrate is the material basis for guaranteeing the high-quality rice seedlings, the problems that the rice seedlings grow slowly, grow too little, die in pieces, are complicated in formula, or are complex to manage and the like often occur in production, and formula screening is performed for the problems, and the key points are as follows: 1 firstly, the substrate must have air permeability, namely the requirement on the porosity of the substrate, the air permeability of the substrate is poor, the activity of aerobic microorganisms is reduced, the water absorption is strong, hardening is caused, the harm to seeds is that root systems are difficult to prick, even if the seeds can be pricked, root retting phenomenon occurs because the oxygen content of the substrate is too low, and finally the substrate grows slowly or dies. 2. The method is characterized in that nutrient elements are balanced, the growth of seedlings needs macroelements and microelements, the macroelements comprise nitrogen, phosphorus and potassium, the microelements comprise boron, iron and zinc, and the like, a certain proportion is required, the imbalance of the nutrient elements is avoided, excessive nitrogen fertilizers are caused, excessive growth is caused, delicate seedlings are formed, in addition, the application of the fertilizer can increase salt ions in a matrix, the normal water absorption of the root system is prevented, and the growth of plants is influenced 3. microbial flora, part of microorganisms in the matrix are probiotics, the probiotics play a good role in soil, the growth environment of the root system is improved, the other part of microorganisms belong to harmful bacteria, the bacteria can cause diseases such as damping off, bakanae disease and the like, the dead seedlings and dead seedlings are caused, the quantity of the harmful bacteria in the matrix is increased along with the extension of planting time, and the imbalance of the soil flora is caused by the fact that the probiotics cannot be supplemented. 4. The pH value and the pH value of the matrix are important for the germination and the growth of the seeds, and the optimal pH value is a neutral environment of about 7.0, so that the germination, the germination and the rooting of the seeds are facilitated.
The planting area of the rice in Tianjin city is about 40 ten thousand mu, the rice is mainly concentrated in Diwu and Ninghe areas, at present, seedling raising substrates are basically purchased from Jiangsu or Hebei and other peripheral areas, the quality of the substrates is uniform, the substrates are common substrates, the effect is single, the price is higher (20 yuan per bag and 50L), the freight cost is higher, chemical agents such as carbendazim, hymexazol and metalaxyl are sprayed in addition to the seed soaking by using medicaments in the seedling raising process, the use cost is increased, the local resources are utilized to develop the seedling raising substrates, the nutrition and disease prevention effects are integrated, and the price is lower than that of products in Jiangsu and other areas.
Disclosure of Invention
The invention aims to provide a microbial seedling culture substrate suitable for rice seedling culture, provides the best substrate condition for the growth of rice in the seedling stage, and simultaneously has the control effect on seedling blight and bakanae disease of the seedling stage of more than 65 percent. .
In order to achieve the purpose, the invention discloses the following technical contents:
a special microbial seedling raising matrix for rice is characterized by comprising the following raw materials:
150 liters of peat soil
50-100L of vermiculite
5-10kg of biochar
1-5L of decomposed chicken manure
River sand 30-40kg
Bacillus subtilis B579 bacterial liquid 1.05X 1010Spore/ml
The seedling substrate particles have the particle size of 1-10mm, the pH value of 6.8-7.3, the conductivity of 51.5-60.4 ms/cm and the spore content of 107/g。
The invention further discloses a preparation method of the substrate for microbial seedling culture, which comprises the following steps:
(1) uniformly mixing and stirring peat soil, vermiculite, biochar, decomposed chicken manure and river sand according to the above proportion;
(2) fermenting and culturing bacillus subtilis B579 in an inclined plane-triangular flask-1 ton fermentation tank-10 ton fermentation tank;
(3) uniformly spraying 50L of bacillus subtilis B579 fermentation culture bacteria liquid 100ml in the step (1), uniformly stirring, bagging, and mixing at present when in use.
The invention further discloses the application of the microbial seedling raising matrix in the aspect of preventing and treating the rice damping off and bakanae disease,
the invention is described in more detail below:
a preparation method of a special microbial seedling substrate for rice comprises the following steps:
(1) uniformly mixing peat soil, vermiculite, biochar, decomposed chicken manure and river sand according to the proportion;
(2) fermenting and culturing bacillus subtilis B579 in a slant-triangular flask (500 ml) -1-ton fermentation tank-10-ton fermentation tank;
1) slant tube culture: potato 200g, glucose (or sucrose) 20g, agar 18g, water 1L, divided into 1.8 x 20cm tubes, 10ml each. Sterilizing at 121 deg.C for 30 min. B579 was inoculated on a slant and cultured at 30 ℃ for 18 h.
2) Culturing in 500ml triangular flask: 200g of potato, 20g of glucose (or sucrose) and 1L of water are subpackaged in 500ml triangular bottles, each bottle is 100ml, and the sterilization is carried out for 30 minutes at the temperature of 121 ℃. Transferring the bacillus subtilis growing on the inclined plane to a triangular flask for continuous culture at 29 ℃ and the rotating speed of 180r/min for 48 h.
3) Culturing in 1 ton fermentation tank with beef extract 3g, peptone 10g, NaCl 5g, and water 1L; pH 7.2, liquid loading of 1 ton fermenter 400L, 33 deg.C, and air flow of 30m3And h, culturing for 6 h.
4) 10 ton fermentation tank: 27% of corn flour, 3.1% of soybean meal, 0.17% of cane sugar, 0.06% of fish meal, 0.08% of magnesium sulfate, 0.003% of potassium dihydrogen phosphate, 0.001% of manganese sulfate, 0.001% of calcium chloride, 0.21% of yeast powder, 0.002% of NaCl0, 4 tons of liquid loading capacity of a 10 ton fermentation tank, 33 ℃ and ventilation capacity of 400m3H, incubation time 19 hours.
The spore content of the bacillus subtilis liquid per milliliter is 1.05 multiplied by 1010cfu, conditions for stepwise amplification culture of bacillus subtilis are as follows:
TABLE 1 conditions for the fermentative production of Bacillus subtilis
(3) Uniformly spraying 50L of bacillus subtilis B579 fermentation culture bacteria liquid 100ml in the step (1), uniformly stirring, bagging, and mixing at present when in use.
The invention mainly solves the nutritional conditions required by rice seedling raising, does not need to add soil or spray nutrient solution, mainly inspects the formula and effect of the seedling raising matrix, has the main difficulty that bacillus subtilis B579 is added, and finally determines the scheme that peat soil, vermiculite, biochar, decomposed chicken manure, river sand and B579 are mixed uniformly according to the proportion.
The invention discloses a preparation method and application of a special seedling raising substrate for rice, which has the following positive effects compared with the prior art:
(1) the microbial seedling raising substrate can prevent and treat damping off and bakanae disease of rice in the seedling stage.
(2) The microbial seedling raising substrate has the advantages of nutrition, air permeability, water retention, disease prevention and the like.
Detailed Description
The invention is described below by means of specific embodiments. Unless otherwise specified, the technical means used in the present invention are well known to those skilled in the art. In addition, the embodiments should be considered illustrative, and not restrictive, of the scope of the invention, which is defined solely by the claims. It will be apparent to those skilled in the art that various changes or modifications in the components and amounts of the materials used in these embodiments can be made without departing from the spirit and scope of the invention. The raw materials and reagents used in the invention, such as peat soil, vermiculite, biochar, decomposed chicken manure, river sand and the like, are commercially available. The separation, screening and identification of the biocontrol bacterium B579 used in the invention are detailed in Shandong agricultural science 2008, 3: 91-94. Various bacterial sources were used:
TABLE 2 sources of the strains
Example 1
A special microbial seedling raising matrix for rice is composed of the following raw materials:
150 liters of peat soil
50 liters of vermiculite
5-kg of biochar
Decomposed chicken manure 1 liter
River sand 30kg
Bacillus subtilis B579 bacterial liquid 1.05X 1010Spore/ml
The seedling substrate particles have the particle size of 1-10mm, the pH value of 6.8-7.3, the conductivity of 51.5-60.4 ms/cm and the spore content of 107/g。
Example 2
Influence of microbial seedling culture substrate on growth of rice plants
TABLE 3 influence of the microbial seedling substrate on Rice seedlings
From the data, the microbial seedling culture medium and the commercial seedling culture medium have no obvious difference on the influence of the rice seedling emergence rate, the plant height, the root length, the stem thickness, the overground fresh weight and the underground fresh weight, and rice seedlings can grow well.
Example 3
Prevention and treatment effect of microbial seedling culture substrate on rice damping-off
TABLE 4 prevention and control effects of the microorganism seedling substrate on rice damping-off
Prevention and treatment effect of microbial seedling culture substrate on rice bakanae disease
TABLE 5 prevention and treatment effects of microorganism seedling raising substrate on bakanae disease of rice
The microbial seedling raising matrix is used for raising rice seedlings, and has a rice damping-off preventing effect of 65.4 percent and a rice bakanae disease preventing effect of 69.3 percent.
Claims (3)
1. A special microbial seedling raising matrix for rice is characterized by comprising the following raw materials:
150 liters of peat soil
50-100L of vermiculite
5-10kg of biochar
1-5L of decomposed chicken manure
River sand 30-40kg
Bacillus subtilis B579 bacterial liquid 1.05X 1010Spore/ml
The seedling substrate particles have the particle size of 1-10mm, the pH value of 6.8-7.3, the conductivity of 51.5-60.4 ms/cm and the spore content of 107/g。
2. A method for preparing a substrate for culturing seedlings of microorganisms as claimed in claim 1, which comprises the following steps:
(1) uniformly mixing and stirring peat soil, vermiculite, biochar, decomposed chicken manure and river sand according to the above proportion;
(2) fermenting and culturing bacillus subtilis B579 in an inclined plane-triangular flask-1 ton fermentation tank-10 ton fermentation tank;
(3) uniformly spraying 50L of bacillus subtilis B579 fermentation culture bacteria liquid 100ml in the step (1), uniformly stirring, bagging, and mixing at present when in use.
3. The application of the microbial seedling raising substrate of claim 1 in the aspect of preventing and treating rice damping-off and bakanae disease.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911391465.3A CN110972897A (en) | 2019-12-30 | 2019-12-30 | Preparation method and application of special microorganism seedling culture substrate for rice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911391465.3A CN110972897A (en) | 2019-12-30 | 2019-12-30 | Preparation method and application of special microorganism seedling culture substrate for rice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110972897A true CN110972897A (en) | 2020-04-10 |
Family
ID=70078718
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911391465.3A Pending CN110972897A (en) | 2019-12-30 | 2019-12-30 | Preparation method and application of special microorganism seedling culture substrate for rice |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110972897A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016455A (en) * | 2021-03-05 | 2021-06-25 | 四川省农业科学院植物保护研究所 | Method for preventing and treating rice damping-off |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1028492A (en) * | 1987-02-19 | 1992-03-19 | Effem Gmbh | Plant substrate and use thereof |
| CN106518401A (en) * | 2016-11-15 | 2017-03-22 | 环境保护部南京环境科学研究所 | Preparation method of mechanical transplanting rice seedling raising substrate |
| CN106995332A (en) * | 2016-01-25 | 2017-08-01 | 北京中农赛世农业科技有限公司 | Rice nursery substrate and rice nursery substrate plate and preparation method thereof |
| CN108887145A (en) * | 2018-09-03 | 2018-11-27 | 天津市植物保护研究所 | A kind of microorganism seedling medium and the preparation method and application thereof |
-
2019
- 2019-12-30 CN CN201911391465.3A patent/CN110972897A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU1028492A (en) * | 1987-02-19 | 1992-03-19 | Effem Gmbh | Plant substrate and use thereof |
| CN106995332A (en) * | 2016-01-25 | 2017-08-01 | 北京中农赛世农业科技有限公司 | Rice nursery substrate and rice nursery substrate plate and preparation method thereof |
| CN106518401A (en) * | 2016-11-15 | 2017-03-22 | 环境保护部南京环境科学研究所 | Preparation method of mechanical transplanting rice seedling raising substrate |
| CN108887145A (en) * | 2018-09-03 | 2018-11-27 | 天津市植物保护研究所 | A kind of microorganism seedling medium and the preparation method and application thereof |
Non-Patent Citations (4)
| Title |
|---|
| 《常用农业科技词浅释》编写组: "《常用农业科技词浅释》", 31 July 1982, 科学普及出版社 * |
| 中国土壤学会: "《土壤科学与生态文明 上》", 30 September 2016 * |
| 中国科学技术学会等: "《园艺学学科发展报告 2014-2015版》", 30 April 2016, 中国科学技术出版社 * |
| 王江柱等: "《常用能用名农药使指南》", 30 September 2008, 金盾出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016455A (en) * | 2021-03-05 | 2021-06-25 | 四川省农业科学院植物保护研究所 | Method for preventing and treating rice damping-off |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101659934B (en) | Antagonistic bacteria preventing and removing continuous cropping banana Panama wilt disease and microbial organic fertilizer thereof | |
| CN111362751B (en) | Special compound microbial disease-resistant growth-promoting bacterial fertilizer for ginseng and preparation method thereof | |
| CN110305671B (en) | Soil biological improver for continuous cropping of greenhouse vegetables and preparation and use methods thereof | |
| CN101671205B (en) | Composite microbial preparation for promoting growth of rape and preparation method thereof | |
| CN105039210A (en) | Complex lively bacterial preparation as well as water soluble fertilizer and application thereof | |
| CN109320345A (en) | It is a kind of for improving the complex micro organism fungicide and purposes of crop yield and quality | |
| CN112501072B (en) | Crop rhizosphere soil conditioner and preparation method and application thereof | |
| CN111440034A (en) | Soil conditioner and preparation method and application thereof | |
| CN106148229B (en) | Streptomyces fradiae solid culture medium, culture method and preparation method of agricultural microbial agent | |
| CN110437000A (en) | A kind of microbial manure and its preparation method and application | |
| CN112679258A (en) | Composite microbial fertilizer and preparation method thereof | |
| CN112225595A (en) | Water-soluble compound microbial fertilizer and preparation method thereof | |
| CN104860732A (en) | Microbial strain organic fertilizer for improving soil alkalinity | |
| CN101659931B (en) | Antagonistic bacteria preventing and removing continuous cropping cucumber rhizoctonia rot and microbial organic fertilizer thereof | |
| CN1029476C (en) | Method for producing azotobacterin | |
| CN103409351A (en) | Growth promoting strain used for promoting banana growth and microbial organic fertilizer produced with same | |
| CN112919982A (en) | Compound microbial agent and fertilizer special for eucalyptus | |
| CN110972897A (en) | Preparation method and application of special microorganism seedling culture substrate for rice | |
| CN111109026A (en) | Biological control method for diseases in rice growth process and application | |
| CN104087539A (en) | Streptomyces microflavus solid fermentation culture medium as well as preparation method and fermentation method thereof | |
| CN108949635B (en) | Bacillus mucilaginosus, microbial fertilizer, preparation method and application thereof | |
| CN111484368A (en) | Solid fermentation production method of microbial fertilizer and solid composite microbial fertilizer | |
| CN102503722A (en) | Composite microbial granules for promoting growth of Pinus massoniana as well as preparation and application methods thereof | |
| CN102701831A (en) | Microbial fertilizer and preparation method thereof | |
| CN111302857B (en) | Water-soluble organic biological bacterial fertilizer for drip irrigation tomatoes and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210220 Address after: 300192, 26 Nankai District Aerospace Road, Tianjin Applicant after: Tianjin Academy of Agricultural Sciences Address before: 300381 in the Academy of Agricultural Sciences, 17 km away from Jinjing Road, Xiqing District, Tianjin Applicant before: TIANJIN INSTITUTE OF PLANT PROTECTION |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200410 |
|
| RJ01 | Rejection of invention patent application after publication |